agarose gel extraction kit  (Jena Bioscience)


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    Name:
    Agarose Gel Extraction Kit
    Description:
    Agarose Gel Extraction Kit is designed for high yield recovery of DNA from agarose gel with simultaneous removal of primer dimers primers nucleotides proteins salt agarose ethidium bromide and other impurities The preparation is based on a silica membrane technology for binding DNA in high salt and elution in low salt buffer The kit provides a simple and efficient way to purify DNA in a size range between 100 bp and 10 kb It requires no organic extractions or precipitation and guarantees high and stable recovery rates
    Catalog Number:
    pp-202l
    Price:
    180.0
    Category:
    Molecular Biology
    Buy from Supplier


    Structured Review

    Jena Bioscience agarose gel extraction kit
    Agarose Gel Extraction Kit is designed for high yield recovery of DNA from agarose gel with simultaneous removal of primer dimers primers nucleotides proteins salt agarose ethidium bromide and other impurities The preparation is based on a silica membrane technology for binding DNA in high salt and elution in low salt buffer The kit provides a simple and efficient way to purify DNA in a size range between 100 bp and 10 kb It requires no organic extractions or precipitation and guarantees high and stable recovery rates
    https://www.bioz.com/result/agarose gel extraction kit/product/Jena Bioscience
    Average 94 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    agarose gel extraction kit - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Human Transbodies to HCV NS3/4A Protease Inhibit Viral Replication and Restore Host Innate Immunity
    Article Snippet: .. The PCR product was stained with ethidium bromide, verified, and purified from agarose gel using DNA extraction kit (Jena Bioscience, Jena, Germany). .. The 5′- and 3′- overhangs of the R9-huscfv amplicons were generated by setting up the following reaction mixtures: 9 μl of sterile distilled water, 2 μl of 5× LIC buffer, 0.1 pmol of the purified PCR product, and 1 μl T4 DNA polymerase and dGTP.

    Electroporation:

    Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylase (GPI12) Gene in Leishmania major (MRHO/IR/75/ER)
    Article Snippet: .. The Linear replacement construct were purified from the gel before electroporation (agarose gel extraction kit, Jena Bioscience, Germany). .. Genotypic analysis of mutant parasites for antibiotic gene integration In the first round of transfection, we expected that one allele of GPI12 was disrupted by NEO construct replacement using the 5′FNEO-3′F cassette.

    Amplification:

    Article Title: Microbial Links between Sulfate Reduction and Metal Retention in Uranium- and Heavy Metal-Contaminated Soil ▿
    Article Snippet: .. The ∼1.9-kb dsrAB amplicon was purified with gel extraction using the agarose gel extraction kit (Jena Bioscience, Jena, Germany) according to the manufacturer's protocol. .. Purified dsrAB amplicons were cloned into the TOPO TA cloning vector pCR4 according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).

    Agarose Gel Electrophoresis:

    Article Title: Production of a kinesin-related recombinant protein (Lbk39) from Leishmania braziliensis by Leishmania tarentolae promastigotes and its application in the serodiagnosis of leishmaniasis
    Article Snippet: .. To confirm the correct procedure for linearization and to isolate the fragment corresponding to the plasmid, 1% agarose gel-isolation of the expression cassette with an Agarose Gel Extraction Kit (Jena Bioscience) was performed according to the manufacturer's instruction. .. The LEXSY strain was previously prepared for transfection according to the LEXSYcon2 Expression Kit manual (for detail see https://www.jenabioscience.com/images/ae3a4f50f1/EGE-1310.pdf ).

    Article Title: Human Transbodies to HCV NS3/4A Protease Inhibit Viral Replication and Restore Host Innate Immunity
    Article Snippet: .. The PCR product was stained with ethidium bromide, verified, and purified from agarose gel using DNA extraction kit (Jena Bioscience, Jena, Germany). .. The 5′- and 3′- overhangs of the R9-huscfv amplicons were generated by setting up the following reaction mixtures: 9 μl of sterile distilled water, 2 μl of 5× LIC buffer, 0.1 pmol of the purified PCR product, and 1 μl T4 DNA polymerase and dGTP.

    Article Title: Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide
    Article Snippet: .. The PCR products were then purified using an agarose gel extraction kit (Jena Bioscience, GmbH, Germany). .. DNA sequencing was carried out by an automated DNA sequencer ABI Prism 3100 - Avant Genetic Analyser (Applied Biosystems, Foster City, CA, USA) with the Big-Dye terminator cycle sequencing kit recommended by the manufacturer (Amersham Pharmacia Biotech, Buckinghamshire, UK).

    Article Title: The crystal structure of the chitinase ChiA74 of Bacillus thuringiensis has a multidomain assembly
    Article Snippet: .. Samples were purified with the agarose gel extraction kit (Jena Biosciences, Jena, Germany) and dialyzed. .. E. coli Top10 was transformed with the purified amplicons by electroporation at 2.5 kV and transformants were selected on LB agar with ampicillin at 37 °C.

    Article Title: Biosafety level-2 laboratory diagnosis of Zaire Ebola virus disease imported from Liberia to Nigeria
    Article Snippet: .. EBOV L-Gene sequencing and phylogenetic analysis PCR amplicons on the agarose gel were purified using an agarose gel extraction kit (Jena Bioscience, Jena, Germany). .. Purified non-infectious PCR products, packaged and transported using triple-level packaging, were sequenced using the Filo A2.3 primer on the Sanger dideoxy sequencing technology platform with an Applied Biosystems 3130xl Genetic Analyser at Jena Bioscience in Jena, Germany.

    Article Title: Microbial Links between Sulfate Reduction and Metal Retention in Uranium- and Heavy Metal-Contaminated Soil ▿
    Article Snippet: .. The ∼1.9-kb dsrAB amplicon was purified with gel extraction using the agarose gel extraction kit (Jena Bioscience, Jena, Germany) according to the manufacturer's protocol. .. Purified dsrAB amplicons were cloned into the TOPO TA cloning vector pCR4 according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).

    Article Title: Biochemical and Molecular Characterization of a Serine Keratinase from Brevibacillus brevis US575 with Promising Keratin-Biodegradation and Hide-Dehairing Activities
    Article Snippet: .. The PCR products were then purified using an agarose gel extraction kit (Jena Bioscience, GmbH, Germany). .. The purified 1.3 kb PCR fragment was cloned in pCR-Blunt cloning vector into E. coli BL21 [F– ompT gal dcm lon hsdSB (rB − mB − ) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5 ] (Invitrogen, Carlsbad, CA, USA) host strain.

    Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylase (GPI12) Gene in Leishmania major (MRHO/IR/75/ER)
    Article Snippet: .. The Linear replacement construct were purified from the gel before electroporation (agarose gel extraction kit, Jena Bioscience, Germany). .. Genotypic analysis of mutant parasites for antibiotic gene integration In the first round of transfection, we expected that one allele of GPI12 was disrupted by NEO construct replacement using the 5′FNEO-3′F cassette.

    Construct:

    Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylase (GPI12) Gene in Leishmania major (MRHO/IR/75/ER)
    Article Snippet: .. The Linear replacement construct were purified from the gel before electroporation (agarose gel extraction kit, Jena Bioscience, Germany). .. Genotypic analysis of mutant parasites for antibiotic gene integration In the first round of transfection, we expected that one allele of GPI12 was disrupted by NEO construct replacement using the 5′FNEO-3′F cassette.

    Purification:

    Article Title: Human Transbodies to HCV NS3/4A Protease Inhibit Viral Replication and Restore Host Innate Immunity
    Article Snippet: .. The PCR product was stained with ethidium bromide, verified, and purified from agarose gel using DNA extraction kit (Jena Bioscience, Jena, Germany). .. The 5′- and 3′- overhangs of the R9-huscfv amplicons were generated by setting up the following reaction mixtures: 9 μl of sterile distilled water, 2 μl of 5× LIC buffer, 0.1 pmol of the purified PCR product, and 1 μl T4 DNA polymerase and dGTP.

    Article Title: Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide
    Article Snippet: .. The PCR products were then purified using an agarose gel extraction kit (Jena Bioscience, GmbH, Germany). .. DNA sequencing was carried out by an automated DNA sequencer ABI Prism 3100 - Avant Genetic Analyser (Applied Biosystems, Foster City, CA, USA) with the Big-Dye terminator cycle sequencing kit recommended by the manufacturer (Amersham Pharmacia Biotech, Buckinghamshire, UK).

    Article Title: The crystal structure of the chitinase ChiA74 of Bacillus thuringiensis has a multidomain assembly
    Article Snippet: .. Samples were purified with the agarose gel extraction kit (Jena Biosciences, Jena, Germany) and dialyzed. .. E. coli Top10 was transformed with the purified amplicons by electroporation at 2.5 kV and transformants were selected on LB agar with ampicillin at 37 °C.

    Article Title: Biosafety level-2 laboratory diagnosis of Zaire Ebola virus disease imported from Liberia to Nigeria
    Article Snippet: .. EBOV L-Gene sequencing and phylogenetic analysis PCR amplicons on the agarose gel were purified using an agarose gel extraction kit (Jena Bioscience, Jena, Germany). .. Purified non-infectious PCR products, packaged and transported using triple-level packaging, were sequenced using the Filo A2.3 primer on the Sanger dideoxy sequencing technology platform with an Applied Biosystems 3130xl Genetic Analyser at Jena Bioscience in Jena, Germany.

    Article Title: Microbial Links between Sulfate Reduction and Metal Retention in Uranium- and Heavy Metal-Contaminated Soil ▿
    Article Snippet: .. The ∼1.9-kb dsrAB amplicon was purified with gel extraction using the agarose gel extraction kit (Jena Bioscience, Jena, Germany) according to the manufacturer's protocol. .. Purified dsrAB amplicons were cloned into the TOPO TA cloning vector pCR4 according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).

    Article Title: Biochemical and Molecular Characterization of a Serine Keratinase from Brevibacillus brevis US575 with Promising Keratin-Biodegradation and Hide-Dehairing Activities
    Article Snippet: .. The PCR products were then purified using an agarose gel extraction kit (Jena Bioscience, GmbH, Germany). .. The purified 1.3 kb PCR fragment was cloned in pCR-Blunt cloning vector into E. coli BL21 [F– ompT gal dcm lon hsdSB (rB − mB − ) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5 ] (Invitrogen, Carlsbad, CA, USA) host strain.

    Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylase (GPI12) Gene in Leishmania major (MRHO/IR/75/ER)
    Article Snippet: .. The Linear replacement construct were purified from the gel before electroporation (agarose gel extraction kit, Jena Bioscience, Germany). .. Genotypic analysis of mutant parasites for antibiotic gene integration In the first round of transfection, we expected that one allele of GPI12 was disrupted by NEO construct replacement using the 5′FNEO-3′F cassette.

    Polymerase Chain Reaction:

    Article Title: Human Transbodies to HCV NS3/4A Protease Inhibit Viral Replication and Restore Host Innate Immunity
    Article Snippet: .. The PCR product was stained with ethidium bromide, verified, and purified from agarose gel using DNA extraction kit (Jena Bioscience, Jena, Germany). .. The 5′- and 3′- overhangs of the R9-huscfv amplicons were generated by setting up the following reaction mixtures: 9 μl of sterile distilled water, 2 μl of 5× LIC buffer, 0.1 pmol of the purified PCR product, and 1 μl T4 DNA polymerase and dGTP.

    Article Title: Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide
    Article Snippet: .. The PCR products were then purified using an agarose gel extraction kit (Jena Bioscience, GmbH, Germany). .. DNA sequencing was carried out by an automated DNA sequencer ABI Prism 3100 - Avant Genetic Analyser (Applied Biosystems, Foster City, CA, USA) with the Big-Dye terminator cycle sequencing kit recommended by the manufacturer (Amersham Pharmacia Biotech, Buckinghamshire, UK).

    Article Title: Biosafety level-2 laboratory diagnosis of Zaire Ebola virus disease imported from Liberia to Nigeria
    Article Snippet: .. EBOV L-Gene sequencing and phylogenetic analysis PCR amplicons on the agarose gel were purified using an agarose gel extraction kit (Jena Bioscience, Jena, Germany). .. Purified non-infectious PCR products, packaged and transported using triple-level packaging, were sequenced using the Filo A2.3 primer on the Sanger dideoxy sequencing technology platform with an Applied Biosystems 3130xl Genetic Analyser at Jena Bioscience in Jena, Germany.

    Article Title: Biochemical and Molecular Characterization of a Serine Keratinase from Brevibacillus brevis US575 with Promising Keratin-Biodegradation and Hide-Dehairing Activities
    Article Snippet: .. The PCR products were then purified using an agarose gel extraction kit (Jena Bioscience, GmbH, Germany). .. The purified 1.3 kb PCR fragment was cloned in pCR-Blunt cloning vector into E. coli BL21 [F– ompT gal dcm lon hsdSB (rB − mB − ) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5 ] (Invitrogen, Carlsbad, CA, USA) host strain.

    Expressing:

    Article Title: Production of a kinesin-related recombinant protein (Lbk39) from Leishmania braziliensis by Leishmania tarentolae promastigotes and its application in the serodiagnosis of leishmaniasis
    Article Snippet: .. To confirm the correct procedure for linearization and to isolate the fragment corresponding to the plasmid, 1% agarose gel-isolation of the expression cassette with an Agarose Gel Extraction Kit (Jena Bioscience) was performed according to the manufacturer's instruction. .. The LEXSY strain was previously prepared for transfection according to the LEXSYcon2 Expression Kit manual (for detail see https://www.jenabioscience.com/images/ae3a4f50f1/EGE-1310.pdf ).

    Staining:

    Article Title: Human Transbodies to HCV NS3/4A Protease Inhibit Viral Replication and Restore Host Innate Immunity
    Article Snippet: .. The PCR product was stained with ethidium bromide, verified, and purified from agarose gel using DNA extraction kit (Jena Bioscience, Jena, Germany). .. The 5′- and 3′- overhangs of the R9-huscfv amplicons were generated by setting up the following reaction mixtures: 9 μl of sterile distilled water, 2 μl of 5× LIC buffer, 0.1 pmol of the purified PCR product, and 1 μl T4 DNA polymerase and dGTP.

    Sequencing:

    Article Title: Biosafety level-2 laboratory diagnosis of Zaire Ebola virus disease imported from Liberia to Nigeria
    Article Snippet: .. EBOV L-Gene sequencing and phylogenetic analysis PCR amplicons on the agarose gel were purified using an agarose gel extraction kit (Jena Bioscience, Jena, Germany). .. Purified non-infectious PCR products, packaged and transported using triple-level packaging, were sequenced using the Filo A2.3 primer on the Sanger dideoxy sequencing technology platform with an Applied Biosystems 3130xl Genetic Analyser at Jena Bioscience in Jena, Germany.

    Gel Extraction:

    Article Title: Microbial Links between Sulfate Reduction and Metal Retention in Uranium- and Heavy Metal-Contaminated Soil ▿
    Article Snippet: .. The ∼1.9-kb dsrAB amplicon was purified with gel extraction using the agarose gel extraction kit (Jena Bioscience, Jena, Germany) according to the manufacturer's protocol. .. Purified dsrAB amplicons were cloned into the TOPO TA cloning vector pCR4 according to the manufacturer's instructions (Invitrogen, Carlsbad, CA).

    Plasmid Preparation:

    Article Title: Production of a kinesin-related recombinant protein (Lbk39) from Leishmania braziliensis by Leishmania tarentolae promastigotes and its application in the serodiagnosis of leishmaniasis
    Article Snippet: .. To confirm the correct procedure for linearization and to isolate the fragment corresponding to the plasmid, 1% agarose gel-isolation of the expression cassette with an Agarose Gel Extraction Kit (Jena Bioscience) was performed according to the manufacturer's instruction. .. The LEXSY strain was previously prepared for transfection according to the LEXSYcon2 Expression Kit manual (for detail see https://www.jenabioscience.com/images/ae3a4f50f1/EGE-1310.pdf ).

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  • 94
    Jena Bioscience agarose gel extraction kit
    Agarose Gel Extraction Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agarose gel extraction kit/product/Jena Bioscience
    Average 94 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    agarose gel extraction kit - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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