poweruptm sybr green master mix  (Thermo Fisher)


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    Thermo Fisher poweruptm sybr green master mix
    Poweruptm Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poweruptm sybr green master mix/product/Thermo Fisher
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    poweruptm sybr green master mix - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: The integrity of the purified RNA was determined using 1.3% agarose gel (containing 1.8 ml formaldehyde and 5 μl SYBR safe) and subjected to electrophoresis at 70 kV for 40 min. RNA samples were stored at −80°C until needed. .. RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system.

    RNA Extraction:

    Article Title: FOXP3 pathogenic variants cause male infertility through affecting the proliferation and apoptosis of human spermatogonial stem cells
    Article Snippet: Paragraph title: RNA extraction, RT-PCR and quantitative real-time PCR ... Real-time PCR reactions were conducted using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, Woolston Warrington, UK) and StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA).

    Amplification:

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system. .. The PCR was programed starting with an initial activation step at 95°C for 2 min, followed by amplification for 40 cycles at 95°C for 15 s, 30 s at various annealing temperatures, depending on the melting temperature of the set of primers , and 72°C for 11 s. The specificity of amplification for each product was determined by a melting curve analysis at 95°C for 5 s and 60°C for 15 s, followed by a progressive increase of the temperature to 95°C with a ramp rate of 0.11°C s–1 , with continued measurement of fluorescence, and finally cooling of the plate at 40°C for 30 s. The housekeeping gene 16S rRNA served as an internal standard.

    Agarose Gel Electrophoresis:

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: The integrity of the purified RNA was determined using 1.3% agarose gel (containing 1.8 ml formaldehyde and 5 μl SYBR safe) and subjected to electrophoresis at 70 kV for 40 min. RNA samples were stored at −80°C until needed. .. RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system.

    Fluorescence:

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system. .. The PCR was programed starting with an initial activation step at 95°C for 2 min, followed by amplification for 40 cycles at 95°C for 15 s, 30 s at various annealing temperatures, depending on the melting temperature of the set of primers , and 72°C for 11 s. The specificity of amplification for each product was determined by a melting curve analysis at 95°C for 5 s and 60°C for 15 s, followed by a progressive increase of the temperature to 95°C with a ramp rate of 0.11°C s–1 , with continued measurement of fluorescence, and finally cooling of the plate at 40°C for 30 s. The housekeeping gene 16S rRNA served as an internal standard.

    Isolation:

    Article Title: FOXP3 pathogenic variants cause male infertility through affecting the proliferation and apoptosis of human spermatogonial stem cells
    Article Snippet: RNA extraction, RT-PCR and quantitative real-time PCR Total RNA was extracted from human SSC line using RNAiso Plus reagent (Takara, Kusatsu, Japan), and the concentrations and quality of isolated RNA were determined by Nanodrop (Thermo Scientific, Massachusetts, USA). .. Real-time PCR reactions were conducted using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, Woolston Warrington, UK) and StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: Paragraph title: RNA Isolation and RT-PCR ... RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system.

    Negative Control:

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: As a negative control similar amount of total RNA was subjected to cDNA synthesis reaction without the reverse transcriptase enzyme. .. RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system.

    Purification:

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: The integrity of the purified RNA was determined using 1.3% agarose gel (containing 1.8 ml formaldehyde and 5 μl SYBR safe) and subjected to electrophoresis at 70 kV for 40 min. RNA samples were stored at −80°C until needed. .. RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system.

    SYBR Green Assay:

    Article Title: FOXP3 pathogenic variants cause male infertility through affecting the proliferation and apoptosis of human spermatogonial stem cells
    Article Snippet: .. Real-time PCR reactions were conducted using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, Woolston Warrington, UK) and StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. The relative expression levels of indicated genes were analyzed via the CT (threshold cycle) value by normalizing with the housekeeping gene ACTB [ΔCT =CT (GENE)−CT (ACTB )], and the changes of gene expression after treatment compared to the control was calculated by formula 2−ΔΔCT [ΔΔCT =ΔCT (treated)−ΔCT (control)].

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: .. RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system. .. The PCR was programed starting with an initial activation step at 95°C for 2 min, followed by amplification for 40 cycles at 95°C for 15 s, 30 s at various annealing temperatures, depending on the melting temperature of the set of primers , and 72°C for 11 s. The specificity of amplification for each product was determined by a melting curve analysis at 95°C for 5 s and 60°C for 15 s, followed by a progressive increase of the temperature to 95°C with a ramp rate of 0.11°C s–1 , with continued measurement of fluorescence, and finally cooling of the plate at 40°C for 30 s. The housekeeping gene 16S rRNA served as an internal standard.

    Activation Assay:

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system. .. The PCR was programed starting with an initial activation step at 95°C for 2 min, followed by amplification for 40 cycles at 95°C for 15 s, 30 s at various annealing temperatures, depending on the melting temperature of the set of primers , and 72°C for 11 s. The specificity of amplification for each product was determined by a melting curve analysis at 95°C for 5 s and 60°C for 15 s, followed by a progressive increase of the temperature to 95°C with a ramp rate of 0.11°C s–1 , with continued measurement of fluorescence, and finally cooling of the plate at 40°C for 30 s. The housekeeping gene 16S rRNA served as an internal standard.

    Expressing:

    Article Title: FOXP3 pathogenic variants cause male infertility through affecting the proliferation and apoptosis of human spermatogonial stem cells
    Article Snippet: Real-time PCR reactions were conducted using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, Woolston Warrington, UK) and StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. The relative expression levels of indicated genes were analyzed via the CT (threshold cycle) value by normalizing with the housekeeping gene ACTB [ΔCT =CT (GENE)−CT (ACTB )], and the changes of gene expression after treatment compared to the control was calculated by formula 2−ΔΔCT [ΔΔCT =ΔCT (treated)−ΔCT (control)].

    Polymerase Chain Reaction:

    Article Title: FOXP3 pathogenic variants cause male infertility through affecting the proliferation and apoptosis of human spermatogonial stem cells
    Article Snippet: The PCR reactions were carried out using Premix Taq™ (TaKaRa Taq™ Version 2.0 plus dye), and the products were analyzed by agarose gels stained with GenGreen nucleic acid gel stain (Genview) and Image Analyzer ChemiDoc XRS+ (Bio-Rad). .. Real-time PCR reactions were conducted using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, Woolston Warrington, UK) and StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system. .. The PCR was programed starting with an initial activation step at 95°C for 2 min, followed by amplification for 40 cycles at 95°C for 15 s, 30 s at various annealing temperatures, depending on the melting temperature of the set of primers , and 72°C for 11 s. The specificity of amplification for each product was determined by a melting curve analysis at 95°C for 5 s and 60°C for 15 s, followed by a progressive increase of the temperature to 95°C with a ramp rate of 0.11°C s–1 , with continued measurement of fluorescence, and finally cooling of the plate at 40°C for 30 s. The housekeeping gene 16S rRNA served as an internal standard.

    Staining:

    Article Title: FOXP3 pathogenic variants cause male infertility through affecting the proliferation and apoptosis of human spermatogonial stem cells
    Article Snippet: The PCR reactions were carried out using Premix Taq™ (TaKaRa Taq™ Version 2.0 plus dye), and the products were analyzed by agarose gels stained with GenGreen nucleic acid gel stain (Genview) and Image Analyzer ChemiDoc XRS+ (Bio-Rad). .. Real-time PCR reactions were conducted using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, Woolston Warrington, UK) and StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: FOXP3 pathogenic variants cause male infertility through affecting the proliferation and apoptosis of human spermatogonial stem cells
    Article Snippet: .. Real-time PCR reactions were conducted using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, Woolston Warrington, UK) and StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). .. The relative expression levels of indicated genes were analyzed via the CT (threshold cycle) value by normalizing with the housekeeping gene ACTB [ΔCT =CT (GENE)−CT (ACTB )], and the changes of gene expression after treatment compared to the control was calculated by formula 2−ΔΔCT [ΔΔCT =ΔCT (treated)−ΔCT (control)].

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: This provides a way to assess the potential DNA contamination of each sample during the real time PCR assay. .. RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system.

    Singleplex Assay:

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: .. RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system. .. The PCR was programed starting with an initial activation step at 95°C for 2 min, followed by amplification for 40 cycles at 95°C for 15 s, 30 s at various annealing temperatures, depending on the melting temperature of the set of primers , and 72°C for 11 s. The specificity of amplification for each product was determined by a melting curve analysis at 95°C for 5 s and 60°C for 15 s, followed by a progressive increase of the temperature to 95°C with a ramp rate of 0.11°C s–1 , with continued measurement of fluorescence, and finally cooling of the plate at 40°C for 30 s. The housekeeping gene 16S rRNA served as an internal standard.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: FOXP3 pathogenic variants cause male infertility through affecting the proliferation and apoptosis of human spermatogonial stem cells
    Article Snippet: Paragraph title: RNA extraction, RT-PCR and quantitative real-time PCR ... Real-time PCR reactions were conducted using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, Woolston Warrington, UK) and StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
    Article Snippet: Paragraph title: RNA Isolation and RT-PCR ... RT-PCRs (Singleplex assay) were performed using PowerUpTM SYBR® Green Master Mix (Applied Biosystems, CA, United States) and Applied Biosystems 7500 Fast system.

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    Thermo Fisher powerup sybr green master mix
    Inhibition assays. Inhibition assays were set up as qPCR reactions using murine genomic DNA; a primer set specific to the mouse- HSD11β1 gene ( S1 Table ); and the <t>PowerUp</t> <t>SYBR</t> Green Master Mix. The reaction mixture was spiked with water (as a control) or extracted RNA or DNA from various kits. Shown are the Cq values for the control vs. reactions spiked with RNA (A) and DNA (B), in duplicate, with error bars representing standard deviations. (C) Fragment distribution of amplified RNA and DNA samples from select kits with corresponding positive controls (fresh PC-3 cells).
    Powerup Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/powerup sybr green master mix/product/Thermo Fisher
    Average 99 stars, based on 503 article reviews
    Price from $9.99 to $1999.99
    powerup sybr green master mix - by Bioz Stars, 2020-04
    99/100 stars
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    Inhibition assays. Inhibition assays were set up as qPCR reactions using murine genomic DNA; a primer set specific to the mouse- HSD11β1 gene ( S1 Table ); and the PowerUp SYBR Green Master Mix. The reaction mixture was spiked with water (as a control) or extracted RNA or DNA from various kits. Shown are the Cq values for the control vs. reactions spiked with RNA (A) and DNA (B), in duplicate, with error bars representing standard deviations. (C) Fragment distribution of amplified RNA and DNA samples from select kits with corresponding positive controls (fresh PC-3 cells).

    Journal: PLoS ONE

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores

    doi: 10.1371/journal.pone.0179732

    Figure Lengend Snippet: Inhibition assays. Inhibition assays were set up as qPCR reactions using murine genomic DNA; a primer set specific to the mouse- HSD11β1 gene ( S1 Table ); and the PowerUp SYBR Green Master Mix. The reaction mixture was spiked with water (as a control) or extracted RNA or DNA from various kits. Shown are the Cq values for the control vs. reactions spiked with RNA (A) and DNA (B), in duplicate, with error bars representing standard deviations. (C) Fragment distribution of amplified RNA and DNA samples from select kits with corresponding positive controls (fresh PC-3 cells).

    Article Snippet: Briefly, standard real-time PCR reactions were set up using murine genomic DNA derived from the Ep4 cell-line as the template; a primer set specific to the HSD11β1 gene ( ); and the PowerUp SYBR Green Master Mix (ThermoFisher Scientific, USA).

    Techniques: Inhibition, Real-time Polymerase Chain Reaction, SYBR Green Assay, Amplification