Structured Review

Tecan Systems powerscanner
Effect of the expression system on the antibody response in NAPPA arrays (A) NAPPA arrays containing 100 unique human genes printed in quadruplicate were expressed with RRL or HCIVT, hybridized with primary antibodies (anti-AKR1B1, ANXA1, CHGA, CRYAB, FABP5, Fascin 1, GPI, Serpin B3, PRDX4, Stratifin or GST) followed by cy3-labeled secondary antibody. Images obtained for 2 of the protein-specific antibodies are shown. All images were acquired using the same settings, with the exception of the anti-GST signal for the array expressed with HCIVT, where the laser power was decreased to prevent saturation. Spots inside the yellow boxes are purified mouse IgG (positive control) and the arrows indicate the antigen spot specific for the antibody tested. (B) The signal intensity of NAPPA arrays was acquired using <t>PowerScanner</t> (Tecan) and Array-ProAnalyzer 6.3 (Tecan). The Z-score of each gene was measured against the average and standard deviation of the raw signal intensity for all gene spots. The dotted line represents the cutoff of 5.
Powerscanner, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 96/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/powerscanner/product/Tecan Systems
Average 96 stars, based on 18 article reviews
Price from $9.99 to $1999.99
powerscanner - by Bioz Stars, 2020-04
96/100 stars

Images

1) Product Images from "Robust microarray production of freshly expressed proteins in a human milieu"

Article Title: Robust microarray production of freshly expressed proteins in a human milieu

Journal: Proteomics. Clinical applications

doi: 10.1002/prca.201200063

Effect of the expression system on the antibody response in NAPPA arrays (A) NAPPA arrays containing 100 unique human genes printed in quadruplicate were expressed with RRL or HCIVT, hybridized with primary antibodies (anti-AKR1B1, ANXA1, CHGA, CRYAB, FABP5, Fascin 1, GPI, Serpin B3, PRDX4, Stratifin or GST) followed by cy3-labeled secondary antibody. Images obtained for 2 of the protein-specific antibodies are shown. All images were acquired using the same settings, with the exception of the anti-GST signal for the array expressed with HCIVT, where the laser power was decreased to prevent saturation. Spots inside the yellow boxes are purified mouse IgG (positive control) and the arrows indicate the antigen spot specific for the antibody tested. (B) The signal intensity of NAPPA arrays was acquired using PowerScanner (Tecan) and Array-ProAnalyzer 6.3 (Tecan). The Z-score of each gene was measured against the average and standard deviation of the raw signal intensity for all gene spots. The dotted line represents the cutoff of 5.
Figure Legend Snippet: Effect of the expression system on the antibody response in NAPPA arrays (A) NAPPA arrays containing 100 unique human genes printed in quadruplicate were expressed with RRL or HCIVT, hybridized with primary antibodies (anti-AKR1B1, ANXA1, CHGA, CRYAB, FABP5, Fascin 1, GPI, Serpin B3, PRDX4, Stratifin or GST) followed by cy3-labeled secondary antibody. Images obtained for 2 of the protein-specific antibodies are shown. All images were acquired using the same settings, with the exception of the anti-GST signal for the array expressed with HCIVT, where the laser power was decreased to prevent saturation. Spots inside the yellow boxes are purified mouse IgG (positive control) and the arrows indicate the antigen spot specific for the antibody tested. (B) The signal intensity of NAPPA arrays was acquired using PowerScanner (Tecan) and Array-ProAnalyzer 6.3 (Tecan). The Z-score of each gene was measured against the average and standard deviation of the raw signal intensity for all gene spots. The dotted line represents the cutoff of 5.

Techniques Used: Expressing, Labeling, Purification, Positive Control, Standard Deviation

Related Articles

Amplification:

Article Title: Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites
Article Snippet: Genomic DNA (3 μg) was first amplified and coupled with aminoallyl-dUTP (Biotium) using Klenow Fragment (3’- 5’exo-) enzyme (New England Biolabs) and random (N9) primers (1 mM). .. Microarrays were incubated at 65 o C in rotator oven (Agilent Technologies) for 20 hours and then scanned using the PowerScanner (Tecan).

Article Title: Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules
Article Snippet: Thereafter, RNA was subjected to two rounds by T7 amplification using the RiboAmp HSPlus RNA Amplification Kit (Life Science, Foster City, CA, USA), purified and quantified using NanoDrop ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA). .. Microarrays slides were then washed and scanned with the PowerScanner (Tecan, Männedorf, Switzerland) and analysed with the Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Whole Genome Amplification:

Article Title: The accuracy of chromosomal microarray testing for identification of embryonic mosaicism in human blastocysts
Article Snippet: CMA (aCGH) The BlueGnome SurePlex and 24sure array kits were used for whole genome amplification (WGA) and subsequent labeling/hybridization of DNAs derived from fibroblast cells and TE specimens, following the manufacturer’s protocol (BlueGnome, UK). .. Slides were scanned using PowerScanner (Tecan, Switzerland) and TIFF images were analyzed and interpreted using log2 ratios calculated by BlueFuse Multi software (BlueGnome, UK).

Protein Array:

Article Title: Effects of pre-existing orthopoxvirus-specific immunity on the performance of Modified Vaccinia virus Ankara-based influenza vaccines
Article Snippet: Paragraph title: Protein Array (PA) assay ... Subsequently, goat-anti-human IgG labelled with AlexaFluor647 (Jackson ImmunoResearch Laboratories Inc.) was used as conjugate and fluorescent signals were measured using a Powerscanner (Tecan Group Ltd).

Blocking Assay:

Article Title: Effects of pre-existing orthopoxvirus-specific immunity on the performance of Modified Vaccinia virus Ankara-based influenza vaccines
Article Snippet: Sera were incubated on the slides in Blotto Blocking Buffer (Thermo Fisher Scientific Inc.) supplemented with 0.1% Surfactant-Amps (Thermo Fisher Scientific Inc.). .. Subsequently, goat-anti-human IgG labelled with AlexaFluor647 (Jackson ImmunoResearch Laboratories Inc.) was used as conjugate and fluorescent signals were measured using a Powerscanner (Tecan Group Ltd).

Microarray:

Article Title: Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites
Article Snippet: Paragraph title: Microarray-based comparative genomic hybridization ... Microarrays were incubated at 65 o C in rotator oven (Agilent Technologies) for 20 hours and then scanned using the PowerScanner (Tecan).

Article Title: Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma
Article Snippet: Paragraph title: Antibody microarray analysis ... The arrays were scanned with identical instrument laser power and adjusted PMT setting using a Powerscanner (Tecan).

Article Title: Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules
Article Snippet: Paragraph title: Microarray analyses ... Microarrays slides were then washed and scanned with the PowerScanner (Tecan, Männedorf, Switzerland) and analysed with the Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Article Title: Antigen Specificity and Clinical Significance of IgG and IgA Autoantibodies Produced in situ by Tumor-Infiltrating B Cells in Breast Cancer
Article Snippet: Paragraph title: Production and processing of antigen microarray ... The arrays were scanned at 10 μm resolution in PowerScanner (Tecan, Switzerland).

Article Title: A Compact, Low-cost, Quantitative and Multiplexed Fluorescence Detection Platform for Point-of-Care Applications
Article Snippet: .. The representative images shown in were acquired using microarray scanner, (PowerScanner, Tecan), typically used in the analysis of high-density DNA and protein microarrays. .. The samples were scanned (PMT gain = 50%; green laser power = 50%) and the images analyzed and quantified with Array-Pro Analyzer software.

Article Title: Mapping transcription factor interactome networks using HaloTag protein arrays
Article Snippet: Paragraph title: Protein–Protein Interaction Assay Using the Microarray Submerge Assay. ... Fluorescence was detected using a PowerScanner (Tecan).

Article Title: The human decapping scavenger enzyme DcpS modulates microRNA turnover
Article Snippet: MiRNA profiles of four non-treated and four treated samples were evaluated using the Unrestricted Human miRNA V19.0 microarray (8 × 60 K; Agilent Technologies). .. After washing, the fluorescent signal intensities were detected on a PowerScanner (Tecan, Mannedorf, Switzerland) and analyzed with Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Incubation:

Article Title: Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites
Article Snippet: .. Microarrays were incubated at 65 o C in rotator oven (Agilent Technologies) for 20 hours and then scanned using the PowerScanner (Tecan). ..

Article Title: Antigen Specificity and Clinical Significance of IgG and IgA Autoantibodies Produced in situ by Tumor-Infiltrating B Cells in Breast Cancer
Article Snippet: The slides were washed overnight and incubated with 1:500 diluted rabbit anti-Halo-tag antibody (G9281, Promega, USA). .. The arrays were scanned at 10 μm resolution in PowerScanner (Tecan, Switzerland).

Article Title: Effects of pre-existing orthopoxvirus-specific immunity on the performance of Modified Vaccinia virus Ankara-based influenza vaccines
Article Snippet: Sera were incubated on the slides in Blotto Blocking Buffer (Thermo Fisher Scientific Inc.) supplemented with 0.1% Surfactant-Amps (Thermo Fisher Scientific Inc.). .. Subsequently, goat-anti-human IgG labelled with AlexaFluor647 (Jackson ImmunoResearch Laboratories Inc.) was used as conjugate and fluorescent signals were measured using a Powerscanner (Tecan Group Ltd).

Article Title: Mapping transcription factor interactome networks using HaloTag protein arrays
Article Snippet: After further washes, arrays were incubated with anti-mouse HRP-coupled secondary antibody [1:500 (vol/vol), GE Healthcare] for 1 h at RT. .. Fluorescence was detected using a PowerScanner (Tecan).

Expressing:

Article Title: Antigen Specificity and Clinical Significance of IgG and IgA Autoantibodies Produced in situ by Tumor-Infiltrating B Cells in Breast Cancer
Article Snippet: Briefly, the slides were blocked in 7% (w/v) milk powder in TBS, 0.05% Tween-20 and incubated with 1:2 diluted tissue culture supernatants or 1:50 diluted plasma samples that had been pre-absorbed with His-Halo-tag expressing E. coli lysates. .. The arrays were scanned at 10 μm resolution in PowerScanner (Tecan, Switzerland).

Article Title: Robust microarray production of freshly expressed proteins in a human milieu
Article Snippet: The transcription/translation was performed with RRL or HCIVT for 1h 30mins at 30°C and the images were obtained in the PowerScanner (Tecan) with 20% gain and 25% laser intensity. .. Both expression systems led to broad display of nearly all proteins on the slides; however, when scanned at settings that are ideal for displaying protein for the RRL system, the signal intensity obtained with HCIVT was saturated ( -insert).

Article Title: The human decapping scavenger enzyme DcpS modulates microRNA turnover
Article Snippet: After washing, the fluorescent signal intensities were detected on a PowerScanner (Tecan, Mannedorf, Switzerland) and analyzed with Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA). .. Agilent miRNA expression arrays were processed using the package limma in Bioconductor .

Derivative Assay:

Article Title: The accuracy of chromosomal microarray testing for identification of embryonic mosaicism in human blastocysts
Article Snippet: CMA (aCGH) The BlueGnome SurePlex and 24sure array kits were used for whole genome amplification (WGA) and subsequent labeling/hybridization of DNAs derived from fibroblast cells and TE specimens, following the manufacturer’s protocol (BlueGnome, UK). .. Slides were scanned using PowerScanner (Tecan, Switzerland) and TIFF images were analyzed and interpreted using log2 ratios calculated by BlueFuse Multi software (BlueGnome, UK).

Article Title: Effects of pre-existing orthopoxvirus-specific immunity on the performance of Modified Vaccinia virus Ankara-based influenza vaccines
Article Snippet: In short, recombinant HA1 derived from influenza viruses A/California/6/2009 (pH1), A/Wyoming/3/2003 (H3 2003), A/Victoria/361/2011 (H3 2011), A/Hong Kong/156/97 (H5 clade 0), A/Vietnam/1194/04 (H5 clade 1), A/Cambodia/R045050/2007 (H5 clade 1.1), A/Indonesia/5/05 (H5 clade 2.1), A/Turkey/15/2006 (H5 clade 2.2), A/Turkey/Germany-MV/R2472/2014 (H5 clade 2.3.4.4), A/goose/Guiyang/337/2006 (H5 clade 4) and A/chicken/Vietnam/NCVD-016/2008 (H5 clade 7), as well as uninfected and wtMVA infected BHK-21 cells lysed in 1% Triton-X100 (Sigma) in PBS supplemented with mini cOmplete™ EDTA free protease inhibitor tablet (Roche) were printed onto nitrocellulose slides by asciFlexarraver (Scienion). .. Subsequently, goat-anti-human IgG labelled with AlexaFluor647 (Jackson ImmunoResearch Laboratories Inc.) was used as conjugate and fluorescent signals were measured using a Powerscanner (Tecan Group Ltd).

Hybridization:

Article Title: Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites
Article Snippet: Paragraph title: Microarray-based comparative genomic hybridization ... Microarrays were incubated at 65 o C in rotator oven (Agilent Technologies) for 20 hours and then scanned using the PowerScanner (Tecan).

Article Title: Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules
Article Snippet: Antisense-RNA (aRNA) samples were labelled with Cy3 or Cy5 using the Universal Linkage System (ULS) kit (Kreatech Diagnostic, Amsterdam, Netherlands) and 825 ng of labelled aRNA were hybridized on the SurePrint G3 Mouse GE 8x60K Microarray kit (Agilent) in a two-color dye-swap design in a hybridization oven for 17 h at 65°C. .. Microarrays slides were then washed and scanned with the PowerScanner (Tecan, Männedorf, Switzerland) and analysed with the Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Article Title: The human decapping scavenger enzyme DcpS modulates microRNA turnover
Article Snippet: Only samples with a RIN equal or superior to 8.5 were selected for microarray hybridization. .. After washing, the fluorescent signal intensities were detected on a PowerScanner (Tecan, Mannedorf, Switzerland) and analyzed with Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Protease Inhibitor:

Article Title: Effects of pre-existing orthopoxvirus-specific immunity on the performance of Modified Vaccinia virus Ankara-based influenza vaccines
Article Snippet: In short, recombinant HA1 derived from influenza viruses A/California/6/2009 (pH1), A/Wyoming/3/2003 (H3 2003), A/Victoria/361/2011 (H3 2011), A/Hong Kong/156/97 (H5 clade 0), A/Vietnam/1194/04 (H5 clade 1), A/Cambodia/R045050/2007 (H5 clade 1.1), A/Indonesia/5/05 (H5 clade 2.1), A/Turkey/15/2006 (H5 clade 2.2), A/Turkey/Germany-MV/R2472/2014 (H5 clade 2.3.4.4), A/goose/Guiyang/337/2006 (H5 clade 4) and A/chicken/Vietnam/NCVD-016/2008 (H5 clade 7), as well as uninfected and wtMVA infected BHK-21 cells lysed in 1% Triton-X100 (Sigma) in PBS supplemented with mini cOmplete™ EDTA free protease inhibitor tablet (Roche) were printed onto nitrocellulose slides by asciFlexarraver (Scienion). .. Subsequently, goat-anti-human IgG labelled with AlexaFluor647 (Jackson ImmunoResearch Laboratories Inc.) was used as conjugate and fluorescent signals were measured using a Powerscanner (Tecan Group Ltd).

Infection:

Article Title: Effects of pre-existing orthopoxvirus-specific immunity on the performance of Modified Vaccinia virus Ankara-based influenza vaccines
Article Snippet: In short, recombinant HA1 derived from influenza viruses A/California/6/2009 (pH1), A/Wyoming/3/2003 (H3 2003), A/Victoria/361/2011 (H3 2011), A/Hong Kong/156/97 (H5 clade 0), A/Vietnam/1194/04 (H5 clade 1), A/Cambodia/R045050/2007 (H5 clade 1.1), A/Indonesia/5/05 (H5 clade 2.1), A/Turkey/15/2006 (H5 clade 2.2), A/Turkey/Germany-MV/R2472/2014 (H5 clade 2.3.4.4), A/goose/Guiyang/337/2006 (H5 clade 4) and A/chicken/Vietnam/NCVD-016/2008 (H5 clade 7), as well as uninfected and wtMVA infected BHK-21 cells lysed in 1% Triton-X100 (Sigma) in PBS supplemented with mini cOmplete™ EDTA free protease inhibitor tablet (Roche) were printed onto nitrocellulose slides by asciFlexarraver (Scienion). .. Subsequently, goat-anti-human IgG labelled with AlexaFluor647 (Jackson ImmunoResearch Laboratories Inc.) was used as conjugate and fluorescent signals were measured using a Powerscanner (Tecan Group Ltd).

Polymerase Chain Reaction:

Article Title: Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites
Article Snippet: The Klenow reaction product (2–3 μg) was then labelled with either Cy5 (GE Healthcare) or Cy3 (GE Healthcare) in the presence of 0.1M NaHCO3 pH 9.0 for 4 hours in the dark, and then purified using the MinElute PCR purification kit (Qiagen). .. Microarrays were incubated at 65 o C in rotator oven (Agilent Technologies) for 20 hours and then scanned using the PowerScanner (Tecan).

Recombinant:

Article Title: Effects of pre-existing orthopoxvirus-specific immunity on the performance of Modified Vaccinia virus Ankara-based influenza vaccines
Article Snippet: In short, recombinant HA1 derived from influenza viruses A/California/6/2009 (pH1), A/Wyoming/3/2003 (H3 2003), A/Victoria/361/2011 (H3 2011), A/Hong Kong/156/97 (H5 clade 0), A/Vietnam/1194/04 (H5 clade 1), A/Cambodia/R045050/2007 (H5 clade 1.1), A/Indonesia/5/05 (H5 clade 2.1), A/Turkey/15/2006 (H5 clade 2.2), A/Turkey/Germany-MV/R2472/2014 (H5 clade 2.3.4.4), A/goose/Guiyang/337/2006 (H5 clade 4) and A/chicken/Vietnam/NCVD-016/2008 (H5 clade 7), as well as uninfected and wtMVA infected BHK-21 cells lysed in 1% Triton-X100 (Sigma) in PBS supplemented with mini cOmplete™ EDTA free protease inhibitor tablet (Roche) were printed onto nitrocellulose slides by asciFlexarraver (Scienion). .. Subsequently, goat-anti-human IgG labelled with AlexaFluor647 (Jackson ImmunoResearch Laboratories Inc.) was used as conjugate and fluorescent signals were measured using a Powerscanner (Tecan Group Ltd).

Fluorescence:

Article Title: A Compact, Low-cost, Quantitative and Multiplexed Fluorescence Detection Platform for Point-of-Care Applications
Article Snippet: To evaluate the performance of our multiplexed fluorescence 2×2 array platform, microscope slides prepared as described above in section 2.3 were inserted into the sample chamber and analyzed. .. The representative images shown in were acquired using microarray scanner, (PowerScanner, Tecan), typically used in the analysis of high-density DNA and protein microarrays.

Article Title: Mapping transcription factor interactome networks using HaloTag protein arrays
Article Snippet: .. Fluorescence was detected using a PowerScanner (Tecan). .. Because the signal-to-noise ratios were very uniform for all features across the glass slide with the submerged assay conditions, we performed all protein–protein interaction assays for the 38 TFs with this method ( ).

Protein Protein Interaction Assay:

Article Title: Mapping transcription factor interactome networks using HaloTag protein arrays
Article Snippet: Paragraph title: Protein–Protein Interaction Assay Using the Microarray Submerge Assay. ... Fluorescence was detected using a PowerScanner (Tecan).

Isolation:

Article Title: The human decapping scavenger enzyme DcpS modulates microRNA turnover
Article Snippet: miRNAs quantification Total RNA, including miRNAs, was isolated with Absolutely RNA miRNA Kit (Agilent Technologies) according to the manufacturer’s protocol. .. After washing, the fluorescent signal intensities were detected on a PowerScanner (Tecan, Mannedorf, Switzerland) and analyzed with Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Microscopy:

Article Title: A Compact, Low-cost, Quantitative and Multiplexed Fluorescence Detection Platform for Point-of-Care Applications
Article Snippet: To ensure that performance of each channel was identical, the 2×2 array was characterized with all four sites containing the same concentration per microscope slide (N=5), for the entire dilution series. .. The representative images shown in were acquired using microarray scanner, (PowerScanner, Tecan), typically used in the analysis of high-density DNA and protein microarrays.

Purification:

Article Title: Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites
Article Snippet: The Klenow reaction product (2–3 μg) was then labelled with either Cy5 (GE Healthcare) or Cy3 (GE Healthcare) in the presence of 0.1M NaHCO3 pH 9.0 for 4 hours in the dark, and then purified using the MinElute PCR purification kit (Qiagen). .. Microarrays were incubated at 65 o C in rotator oven (Agilent Technologies) for 20 hours and then scanned using the PowerScanner (Tecan).

Article Title: Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules
Article Snippet: Thereafter, RNA was subjected to two rounds by T7 amplification using the RiboAmp HSPlus RNA Amplification Kit (Life Science, Foster City, CA, USA), purified and quantified using NanoDrop ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA). .. Microarrays slides were then washed and scanned with the PowerScanner (Tecan, Männedorf, Switzerland) and analysed with the Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Labeling:

Article Title: The accuracy of chromosomal microarray testing for identification of embryonic mosaicism in human blastocysts
Article Snippet: CMA (aCGH) The BlueGnome SurePlex and 24sure array kits were used for whole genome amplification (WGA) and subsequent labeling/hybridization of DNAs derived from fibroblast cells and TE specimens, following the manufacturer’s protocol (BlueGnome, UK). .. Slides were scanned using PowerScanner (Tecan, Switzerland) and TIFF images were analyzed and interpreted using log2 ratios calculated by BlueFuse Multi software (BlueGnome, UK).

Article Title: The human decapping scavenger enzyme DcpS modulates microRNA turnover
Article Snippet: Briefly, 100 ng of total RNA were treated with alkaline calf intestine phosphatase and labeled with Cyanine 3-pCp. .. After washing, the fluorescent signal intensities were detected on a PowerScanner (Tecan, Mannedorf, Switzerland) and analyzed with Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Chromatin Immunoprecipitation:

Article Title: Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites
Article Snippet: Equal amounts of Cy5-labelled sample DNA and Cy3-labelled reference DNA were then mixed with 2x Hybridization Buffer (Agilent Technologies) and deposited onto a microarray chip containing 10,367 probes covering over 5,000 genes in the Plasmodium falciparum genome. .. Microarrays were incubated at 65 o C in rotator oven (Agilent Technologies) for 20 hours and then scanned using the PowerScanner (Tecan).

Article Title: Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules
Article Snippet: Quality and concentration of extracted RNA was measured using the 2100-Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) with the RNA PicoLab Chip (Agilent Technologies). .. Microarrays slides were then washed and scanned with the PowerScanner (Tecan, Männedorf, Switzerland) and analysed with the Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Software:

Article Title: Protein Profiling Gastric Cancer and Neighboring Control Tissues Using High-Content Antibody Microarrays
Article Snippet: Data Analysis and Sample Classification Scanning of the slides was performed with a PowerScanner (Tecan) at a resolution of 10 μm, maintaining laser power and the photomultiplier constant. .. Spot segmentation was performed with GenePix Pro 6.0 software package (Molecular Devices, Sunnyvale, CA, USA).

Article Title: Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma
Article Snippet: The arrays were scanned with identical instrument laser power and adjusted PMT setting using a Powerscanner (Tecan). .. Spot segmentation was performed with the software GenePix Pro 6.0 (Molecular Devices).

Article Title: Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules
Article Snippet: .. Microarrays slides were then washed and scanned with the PowerScanner (Tecan, Männedorf, Switzerland) and analysed with the Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA). .. Microarray data were pre-processed and analysed using the FlexArray 1.6.1 ( http://genomequebec.mcgill.ca/FlexArray ).

Article Title: The accuracy of chromosomal microarray testing for identification of embryonic mosaicism in human blastocysts
Article Snippet: .. Slides were scanned using PowerScanner (Tecan, Switzerland) and TIFF images were analyzed and interpreted using log2 ratios calculated by BlueFuse Multi software (BlueGnome, UK). .. Embryos identified to be aneuploid (non-mosaic or mosaic) by CMA from TE specimens were reanalyzed, when available, by FISH to confirm the array diagnosis.

Article Title: Antigen Specificity and Clinical Significance of IgG and IgA Autoantibodies Produced in situ by Tumor-Infiltrating B Cells in Breast Cancer
Article Snippet: The arrays were scanned at 10 μm resolution in PowerScanner (Tecan, Switzerland). .. The results were recorded as TIFF files and the raw data were processed with GenePix™ Pro 4.0 software (Molecular Devices, USA) and further analyzed using an ad hoc program composed in R language.

Article Title: A Compact, Low-cost, Quantitative and Multiplexed Fluorescence Detection Platform for Point-of-Care Applications
Article Snippet: The representative images shown in were acquired using microarray scanner, (PowerScanner, Tecan), typically used in the analysis of high-density DNA and protein microarrays. .. The samples were scanned (PMT gain = 50%; green laser power = 50%) and the images analyzed and quantified with Array-Pro Analyzer software.

Article Title: The human decapping scavenger enzyme DcpS modulates microRNA turnover
Article Snippet: .. After washing, the fluorescent signal intensities were detected on a PowerScanner (Tecan, Mannedorf, Switzerland) and analyzed with Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA). .. To evaluate the efficiency of labeling and hybridization, Agilent microRNA Spike-In were added in the appropriate steps.

Homogenization:

Article Title: Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma
Article Snippet: After sample homogenisation, proteins were extracted with scioExtract buffer (Sciomics) and labelled at an adjusted concentration with scioDye 2 (Sciomics) according to the manufacturer´s instructions. .. The arrays were scanned with identical instrument laser power and adjusted PMT setting using a Powerscanner (Tecan).

Spectrophotometry:

Article Title: Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules
Article Snippet: Thereafter, RNA was subjected to two rounds by T7 amplification using the RiboAmp HSPlus RNA Amplification Kit (Life Science, Foster City, CA, USA), purified and quantified using NanoDrop ND-1000 spectrophotometer (NanoDrop, Wilmington, DE, USA). .. Microarrays slides were then washed and scanned with the PowerScanner (Tecan, Männedorf, Switzerland) and analysed with the Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Produced:

Article Title: Robust microarray production of freshly expressed proteins in a human milieu
Article Snippet: The capture and detection antibodies were produced in distinct hosts and recognize distinct epitopes in the GST tag, allowing them to be used simultaneously. .. The transcription/translation was performed with RRL or HCIVT for 1h 30mins at 30°C and the images were obtained in the PowerScanner (Tecan) with 20% gain and 25% laser intensity.

Concentration Assay:

Article Title: Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma
Article Snippet: After sample homogenisation, proteins were extracted with scioExtract buffer (Sciomics) and labelled at an adjusted concentration with scioDye 2 (Sciomics) according to the manufacturer´s instructions. .. The arrays were scanned with identical instrument laser power and adjusted PMT setting using a Powerscanner (Tecan).

Article Title: Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules
Article Snippet: Quality and concentration of extracted RNA was measured using the 2100-Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) with the RNA PicoLab Chip (Agilent Technologies). .. Microarrays slides were then washed and scanned with the PowerScanner (Tecan, Männedorf, Switzerland) and analysed with the Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Article Title: Effects of pre-existing orthopoxvirus-specific immunity on the performance of Modified Vaccinia virus Ankara-based influenza vaccines
Article Snippet: Subsequently, goat-anti-human IgG labelled with AlexaFluor647 (Jackson ImmunoResearch Laboratories Inc.) was used as conjugate and fluorescent signals were measured using a Powerscanner (Tecan Group Ltd). .. The titer of each serum sample was defined as the interpolated serum concentration generating the 50% point using a four-parameter logistic nonlinear regression model using R (R Statistical Computing, version 3.1.0.

Article Title: A Compact, Low-cost, Quantitative and Multiplexed Fluorescence Detection Platform for Point-of-Care Applications
Article Snippet: To ensure that performance of each channel was identical, the 2×2 array was characterized with all four sites containing the same concentration per microscope slide (N=5), for the entire dilution series. .. The representative images shown in were acquired using microarray scanner, (PowerScanner, Tecan), typically used in the analysis of high-density DNA and protein microarrays.

Article Title: The human decapping scavenger enzyme DcpS modulates microRNA turnover
Article Snippet: RNA concentration, purity and RNA integrity number (RIN) were determined on an Agilent 2100 Bioanalyzer and RNA 6000 NanoLabChip Kits (Agilent Technologies). .. After washing, the fluorescent signal intensities were detected on a PowerScanner (Tecan, Mannedorf, Switzerland) and analyzed with Array-Pro Analyzer software (MediaCybernetics, Bethesda, MD, USA).

Fluorescence In Situ Hybridization:

Article Title: The accuracy of chromosomal microarray testing for identification of embryonic mosaicism in human blastocysts
Article Snippet: Slides were scanned using PowerScanner (Tecan, Switzerland) and TIFF images were analyzed and interpreted using log2 ratios calculated by BlueFuse Multi software (BlueGnome, UK). .. Embryos identified to be aneuploid (non-mosaic or mosaic) by CMA from TE specimens were reanalyzed, when available, by FISH to confirm the array diagnosis.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Tecan Systems powerscanner
    Effect of the expression system on the antibody response in NAPPA arrays (A) NAPPA arrays containing 100 unique human genes printed in quadruplicate were expressed with RRL or HCIVT, hybridized with primary antibodies (anti-AKR1B1, ANXA1, CHGA, CRYAB, FABP5, Fascin 1, GPI, Serpin B3, PRDX4, Stratifin or GST) followed by cy3-labeled secondary antibody. Images obtained for 2 of the protein-specific antibodies are shown. All images were acquired using the same settings, with the exception of the anti-GST signal for the array expressed with HCIVT, where the laser power was decreased to prevent saturation. Spots inside the yellow boxes are purified mouse IgG (positive control) and the arrows indicate the antigen spot specific for the antibody tested. (B) The signal intensity of NAPPA arrays was acquired using <t>PowerScanner</t> (Tecan) and Array-ProAnalyzer 6.3 (Tecan). The Z-score of each gene was measured against the average and standard deviation of the raw signal intensity for all gene spots. The dotted line represents the cutoff of 5.
    Powerscanner, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 96/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/powerscanner/product/Tecan Systems
    Average 96 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    powerscanner - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    91
    Tecan Systems tecan powerscanner device
    Effect of the expression system on the antibody response in NAPPA arrays (A) NAPPA arrays containing 100 unique human genes printed in quadruplicate were expressed with RRL or HCIVT, hybridized with primary antibodies (anti-AKR1B1, ANXA1, CHGA, CRYAB, FABP5, Fascin 1, GPI, Serpin B3, PRDX4, Stratifin or GST) followed by cy3-labeled secondary antibody. Images obtained for 2 of the protein-specific antibodies are shown. All images were acquired using the same settings, with the exception of the anti-GST signal for the array expressed with HCIVT, where the laser power was decreased to prevent saturation. Spots inside the yellow boxes are purified mouse IgG (positive control) and the arrows indicate the antigen spot specific for the antibody tested. (B) The signal intensity of NAPPA arrays was acquired using <t>PowerScanner</t> (Tecan) and Array-ProAnalyzer 6.3 (Tecan). The Z-score of each gene was measured against the average and standard deviation of the raw signal intensity for all gene spots. The dotted line represents the cutoff of 5.
    Tecan Powerscanner Device, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tecan powerscanner device/product/Tecan Systems
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tecan powerscanner device - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    95
    Tecan Systems powerscanner confocal laser scanner
    Effect of the expression system on the antibody response in NAPPA arrays (A) NAPPA arrays containing 100 unique human genes printed in quadruplicate were expressed with RRL or HCIVT, hybridized with primary antibodies (anti-AKR1B1, ANXA1, CHGA, CRYAB, FABP5, Fascin 1, GPI, Serpin B3, PRDX4, Stratifin or GST) followed by cy3-labeled secondary antibody. Images obtained for 2 of the protein-specific antibodies are shown. All images were acquired using the same settings, with the exception of the anti-GST signal for the array expressed with HCIVT, where the laser power was decreased to prevent saturation. Spots inside the yellow boxes are purified mouse IgG (positive control) and the arrows indicate the antigen spot specific for the antibody tested. (B) The signal intensity of NAPPA arrays was acquired using <t>PowerScanner</t> (Tecan) and Array-ProAnalyzer 6.3 (Tecan). The Z-score of each gene was measured against the average and standard deviation of the raw signal intensity for all gene spots. The dotted line represents the cutoff of 5.
    Powerscanner Confocal Laser Scanner, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/powerscanner confocal laser scanner/product/Tecan Systems
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    powerscanner confocal laser scanner - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Effect of the expression system on the antibody response in NAPPA arrays (A) NAPPA arrays containing 100 unique human genes printed in quadruplicate were expressed with RRL or HCIVT, hybridized with primary antibodies (anti-AKR1B1, ANXA1, CHGA, CRYAB, FABP5, Fascin 1, GPI, Serpin B3, PRDX4, Stratifin or GST) followed by cy3-labeled secondary antibody. Images obtained for 2 of the protein-specific antibodies are shown. All images were acquired using the same settings, with the exception of the anti-GST signal for the array expressed with HCIVT, where the laser power was decreased to prevent saturation. Spots inside the yellow boxes are purified mouse IgG (positive control) and the arrows indicate the antigen spot specific for the antibody tested. (B) The signal intensity of NAPPA arrays was acquired using PowerScanner (Tecan) and Array-ProAnalyzer 6.3 (Tecan). The Z-score of each gene was measured against the average and standard deviation of the raw signal intensity for all gene spots. The dotted line represents the cutoff of 5.

    Journal: Proteomics. Clinical applications

    Article Title: Robust microarray production of freshly expressed proteins in a human milieu

    doi: 10.1002/prca.201200063

    Figure Lengend Snippet: Effect of the expression system on the antibody response in NAPPA arrays (A) NAPPA arrays containing 100 unique human genes printed in quadruplicate were expressed with RRL or HCIVT, hybridized with primary antibodies (anti-AKR1B1, ANXA1, CHGA, CRYAB, FABP5, Fascin 1, GPI, Serpin B3, PRDX4, Stratifin or GST) followed by cy3-labeled secondary antibody. Images obtained for 2 of the protein-specific antibodies are shown. All images were acquired using the same settings, with the exception of the anti-GST signal for the array expressed with HCIVT, where the laser power was decreased to prevent saturation. Spots inside the yellow boxes are purified mouse IgG (positive control) and the arrows indicate the antigen spot specific for the antibody tested. (B) The signal intensity of NAPPA arrays was acquired using PowerScanner (Tecan) and Array-ProAnalyzer 6.3 (Tecan). The Z-score of each gene was measured against the average and standard deviation of the raw signal intensity for all gene spots. The dotted line represents the cutoff of 5.

    Article Snippet: The transcription/translation was performed with RRL or HCIVT for 1h 30mins at 30°C and the images were obtained in the PowerScanner (Tecan) with 20% gain and 25% laser intensity.

    Techniques: Expressing, Labeling, Purification, Positive Control, Standard Deviation