power sybr green pcr master mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher power sybr green pcr master mix
    PVC-CM reduces inflammation and ER stress in in macrophages of MSU-induced peritonitis. (A) Schematic diagram of experiments using the peritonitis mouse model (n=8 per group). (B) PECs were determined by counting exudate cells with a cell counter. (C) Cytospins of isolated PECs were stained with Wright-Giemsa stain. (D, E) Relative mRNA levels of inflammatory (IL-1 α , IL-1 β , NLRP3, and Casp1) and ER stress-related genes (ATF6, PERK, elk2 α , and IRE-1) were analyzed using <t>SYBR</t> green-based quantitative real-time <t>PCR.</t> Error bars indicate SD (*p
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 6467 article reviews
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    power sybr green pcr master mix - by Bioz Stars, 2020-01
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    1) Product Images from "Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages"

    Article Title: Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages

    Journal: International Journal of Stem Cells

    doi: 10.15283/ijsc19115

    PVC-CM reduces inflammation and ER stress in in macrophages of MSU-induced peritonitis. (A) Schematic diagram of experiments using the peritonitis mouse model (n=8 per group). (B) PECs were determined by counting exudate cells with a cell counter. (C) Cytospins of isolated PECs were stained with Wright-Giemsa stain. (D, E) Relative mRNA levels of inflammatory (IL-1 α , IL-1 β , NLRP3, and Casp1) and ER stress-related genes (ATF6, PERK, elk2 α , and IRE-1) were analyzed using SYBR green-based quantitative real-time PCR. Error bars indicate SD (*p
    Figure Legend Snippet: PVC-CM reduces inflammation and ER stress in in macrophages of MSU-induced peritonitis. (A) Schematic diagram of experiments using the peritonitis mouse model (n=8 per group). (B) PECs were determined by counting exudate cells with a cell counter. (C) Cytospins of isolated PECs were stained with Wright-Giemsa stain. (D, E) Relative mRNA levels of inflammatory (IL-1 α , IL-1 β , NLRP3, and Casp1) and ER stress-related genes (ATF6, PERK, elk2 α , and IRE-1) were analyzed using SYBR green-based quantitative real-time PCR. Error bars indicate SD (*p

    Techniques Used: Isolation, Staining, Giemsa Stain, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Related Articles

    Amplification:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no. .. 4367659), and run using the 7900HT Fast Real-Time PCR System (ThermoFischer), with initial run for 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of denaturation at 95°C (15 sec) and amplification at 60°C (1 min).

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: The amplified products were separated on a 3% agarose gel and visualized by ethidium bromide staining. .. PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix.

    Article Title: In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing
    Article Snippet: Synthesized cDNA samples were diluted (10×), stored at −80°C, and thawed only once, just before the amplification. .. Reactions were carried out in final volumes of 20 µL, consisting of 10 µL of Power SYBR Green PCR Master Mix (Life Technologies, USA), 0.25 µM of each primer (forward and reverse; Supplementary Table 1), 1 µL of cDNA template and 7 µL of PCR-grade water.

    Article Title: The Role of Aberrant DNA Methylation in Misregulation of Gene Expression in Gonadotroph Nonfunctioning Pituitary Tumors
    Article Snippet: .. Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) was used for amplification. .. PCRs were run in 5-mL volumes containing 2.25 pmol of each primer on a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) in 384-well format.

    Article Title: Elevated aldosterone and blood pressure in a mouse model of familial hyperaldosteronism with ClC-2 mutation
    Article Snippet: .. Gapdh (primers 5′-TGTAGACCATGTAGTTGAGGTCA-3′ and 5′-AGGTCGGTGTGAACGGATTTG-3′), Ren1 (5′-TCTGGGCACTCTTGTTGCTC-3′ and 5′-GGGGGAGGTAAGATTGGTCAA-3′), Slc13a2 (5′-CGAGAGTAATCCAGCAGTA-3′ and 5′-ATGAAGAGATTAACAAGAACAGAA-3′) and Sgk1 (5′-ATGCAGTAAACCAGCCGGT-3′ and 5′-TGATCCATCTTCGTACCCGT-3′) were amplified from kidney cDNA using Power SYBR Green PCR Master Mix (Applied Biosystems). .. Gene expression was evaluated relative to Gapdh , and mean ΔCT of WT controls and expressed as 2ΔΔCt (fold change).

    DNA Synthesis:

    Article Title: Remodeling of the collagen matrix in aging skin promotes melanoma metastasis and affects immune cell motility
    Article Snippet: 1μg RNA was used to prepare cDNA using iscript DNA synthesis kit (#1708891, Bio-Rad, CA). cDNA was diluted 1:5 before use for further reactions. .. Each 20μl well reaction comprised of 10μl Power SYBR Green Master mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA.

    Synthesized:

    Article Title: In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing
    Article Snippet: Synthesized cDNA samples were diluted (10×), stored at −80°C, and thawed only once, just before the amplification. .. Reactions were carried out in final volumes of 20 µL, consisting of 10 µL of Power SYBR Green PCR Master Mix (Life Technologies, USA), 0.25 µM of each primer (forward and reverse; Supplementary Table 1), 1 µL of cDNA template and 7 µL of PCR-grade water.

    Article Title: Aberrant hypermethylation-mediated downregulation of antisense lncRNA ZNF667-AS1 and its sense gene ZNF667 correlate with progression and prognosis of esophageal squamous cell carcinoma
    Article Snippet: First-strand cDNAs were synthesized from 2 μg of the total RNA using a High-Capacity cDNA Reverse Transcription kit according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). .. Quantitative real-time PCR was performed with Power SYBR Green PCR Master Mix (Life Technology, Foster City, CA, USA).

    Article Title: Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
    Article Snippet: Single-stranded cDNA was synthesized with miScript II RT Kit (Qiagen, Germany). .. QPCR was performed by ViiA 7 sequence detection system using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and specific primers.

    Quantitative RT-PCR:

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: Paragraph title: Total RNA isolation, semi-quantitative RT-PCR of miRNAs and Quantitative RT-PCR ... PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix.

    Article Title: Aberrant hypermethylation-mediated downregulation of antisense lncRNA ZNF667-AS1 and its sense gene ZNF667 correlate with progression and prognosis of esophageal squamous cell carcinoma
    Article Snippet: Paragraph title: RNA extraction and quantitative real-time RT-PCR (qRT-PCR) assay ... Quantitative real-time PCR was performed with Power SYBR Green PCR Master Mix (Life Technology, Foster City, CA, USA).

    Article Title: The Role of Aberrant DNA Methylation in Misregulation of Gene Expression in Gonadotroph Nonfunctioning Pituitary Tumors
    Article Snippet: Paragraph title: 4.5. qRT-PCR Gene Expression Assessment ... Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) was used for amplification.

    Real-time Polymerase Chain Reaction:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: .. Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no. .. 4367659), and run using the 7900HT Fast Real-Time PCR System (ThermoFischer), with initial run for 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of denaturation at 95°C (15 sec) and amplification at 60°C (1 min).

    Article Title: Remodeling of the collagen matrix in aging skin promotes melanoma metastasis and affects immune cell motility
    Article Snippet: Paragraph title: Quantitative PCR ... Each 20μl well reaction comprised of 10μl Power SYBR Green Master mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA.

    Article Title: In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing
    Article Snippet: Real-time PCR was used to determine the MiR92b-3p levels in the cDNA samples. .. Reactions were carried out in final volumes of 20 µL, consisting of 10 µL of Power SYBR Green PCR Master Mix (Life Technologies, USA), 0.25 µM of each primer (forward and reverse; Supplementary Table 1), 1 µL of cDNA template and 7 µL of PCR-grade water.

    Article Title: Aberrant hypermethylation-mediated downregulation of antisense lncRNA ZNF667-AS1 and its sense gene ZNF667 correlate with progression and prognosis of esophageal squamous cell carcinoma
    Article Snippet: .. Quantitative real-time PCR was performed with Power SYBR Green PCR Master Mix (Life Technology, Foster City, CA, USA). .. Relative gene expression was determined by 2–ΔΔCT method normalized to GAPDH .

    Article Title: Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis
    Article Snippet: Paragraph title: Quantitative PCR ... Each 20μl reaction comprised 1μl Power SYBR Green Master Mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA.

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    Article Snippet: .. QPCR was performed by ViiA 7 sequence detection system using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and specific primers. .. Gene expression was assessed by the standard curve method and normalized to the reference gene beta-glucuronidase (GUSB).

    Article Title: Causative role of PDLIM2 epigenetic repression in lung cancer and therapeutic resistance
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) analysis The indicated tissues or cells were subjected to RNA extraction, RNA reverse transcription and real-time PCR using trizol, reverse transcriptase, and Power SYBR Green PCR Master Mix (Thermo Fisher Scientific, Waltham, MA USA) according to the product manufacture’s protocol , . .. Primer pairs used for qPCR were listed in Supplementary Table .

    Article Title: Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages
    Article Snippet: .. Transcripts were quantitated using Power SYBR® Green PCR Master Mix (Applied Biosystems, Carlsbad, CA, USA) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Article Title: Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia
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    Article Title: Elevated aldosterone and blood pressure in a mouse model of familial hyperaldosteronism with ClC-2 mutation
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    Quantitation Assay:

    Article Title: Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages
    Article Snippet: Transcripts were quantitated using Power SYBR® Green PCR Master Mix (Applied Biosystems, Carlsbad, CA, USA) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). .. All of the quantitation was normalized with β -actin.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
    Article Snippet: Gene expression We evaluated BAX and Bcl2 gene expression in formalin-fixed, paraffin-embedded tissues from samples collected at D0 and D21. mRNA was extracted using a commercial RecoverAll™ Total Nucleic Acid Kit (Ambion, Life Technologies, MA, USA) according to the manufacturer’s instructions. .. The BAX and Bcl2 primers as well as the reference gene primers were designed as previously described and added to a final concentration of 0.3 μM for each primer in a total volume of 10 μL containing Power SYBR Green PCR Master Mix (Applied Biosystems; Foster City, CA, USA), 1 μL of cDNA (1:10).

    In Silico:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no. .. Primers were designed using NCBI PrimerBlast, and specificity tested in silico by BLAST.

    Expressing:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no. .. All samples were normalized to Cd45 expression.

    Article Title: Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
    Article Snippet: Paragraph title: Gene expression ... The BAX and Bcl2 primers as well as the reference gene primers were designed as previously described and added to a final concentration of 0.3 μM for each primer in a total volume of 10 μL containing Power SYBR Green PCR Master Mix (Applied Biosystems; Foster City, CA, USA), 1 μL of cDNA (1:10).

    Article Title: Aberrant hypermethylation-mediated downregulation of antisense lncRNA ZNF667-AS1 and its sense gene ZNF667 correlate with progression and prognosis of esophageal squamous cell carcinoma
    Article Snippet: Quantitative real-time PCR was performed with Power SYBR Green PCR Master Mix (Life Technology, Foster City, CA, USA). .. Relative gene expression was determined by 2–ΔΔCT method normalized to GAPDH .

    Article Title: The Role of Aberrant DNA Methylation in Misregulation of Gene Expression in Gonadotroph Nonfunctioning Pituitary Tumors
    Article Snippet: Paragraph title: 4.5. qRT-PCR Gene Expression Assessment ... Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) was used for amplification.

    Article Title: Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
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    Article Title: Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia
    Article Snippet: .. The expression of EZH1 and other genes was measured using Power SYBR® Green PCR Master Mix (Applied Biosystems). .. Expression of all genes was determined by the comparative Ct method using ABL1 or GAPDH levels for normalization.

    Article Title: Elevated aldosterone and blood pressure in a mouse model of familial hyperaldosteronism with ClC-2 mutation
    Article Snippet: After reverse transcription of RNA using Quantitect RT Kit (Qiagen), Taqman gene expression assays (Applied Biosystems) for Gapdh (Mm99999915_g1, housekeeping gene), Cyp11b2 (Mm00515624_m1) and Clcn2 (Mm01344259_m1) were performed for adrenal samples. .. Gapdh (primers 5′-TGTAGACCATGTAGTTGAGGTCA-3′ and 5′-AGGTCGGTGTGAACGGATTTG-3′), Ren1 (5′-TCTGGGCACTCTTGTTGCTC-3′ and 5′-GGGGGAGGTAAGATTGGTCAA-3′), Slc13a2 (5′-CGAGAGTAATCCAGCAGTA-3′ and 5′-ATGAAGAGATTAACAAGAACAGAA-3′) and Sgk1 (5′-ATGCAGTAAACCAGCCGGT-3′ and 5′-TGATCCATCTTCGTACCCGT-3′) were amplified from kidney cDNA using Power SYBR Green PCR Master Mix (Applied Biosystems).

    Sequencing:

    Article Title: Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
    Article Snippet: .. QPCR was performed by ViiA 7 sequence detection system using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and specific primers. .. Gene expression was assessed by the standard curve method and normalized to the reference gene beta-glucuronidase (GUSB).

    Generated:

    Article Title: Remodeling of the collagen matrix in aging skin promotes melanoma metastasis and affects immune cell motility
    Article Snippet: Each 20μl well reaction comprised of 10μl Power SYBR Green Master mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA. .. Standard curves were generated for all primers and each set of primers were normalized to 18 Primer pair (#AM1718, Ambion, Invitrogen).

    Article Title: Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis
    Article Snippet: Each 20μl reaction comprised 1μl Power SYBR Green Master Mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA. .. Standard curves were generated for each primer and used to perform relative quantification.

    Polymerase Chain Reaction:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: .. Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no. .. 4367659), and run using the 7900HT Fast Real-Time PCR System (ThermoFischer), with initial run for 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of denaturation at 95°C (15 sec) and amplification at 60°C (1 min).

    Article Title: Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
    Article Snippet: .. The BAX and Bcl2 primers as well as the reference gene primers were designed as previously described and added to a final concentration of 0.3 μM for each primer in a total volume of 10 μL containing Power SYBR Green PCR Master Mix (Applied Biosystems; Foster City, CA, USA), 1 μL of cDNA (1:10). .. The reactions were performed in triplicate in 384-well plates using QuantStudio 12 K Flex Thermal Cycler equipment (Applied Biosystems; Foster City, CA, USA).

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: .. PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix. .. PCR signals were detected using CFX Connect (Bio-Rad) and the cycling conditions; 50°C for 2 min, 95°C for 10 min; 40 cycles (95°C, 15 sec; 60°C, 1 min).

    Article Title: In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing
    Article Snippet: .. Reactions were carried out in final volumes of 20 µL, consisting of 10 µL of Power SYBR Green PCR Master Mix (Life Technologies, USA), 0.25 µM of each primer (forward and reverse; Supplementary Table 1), 1 µL of cDNA template and 7 µL of PCR-grade water. .. Amplification was performed with an ABI 7500 Real-time PCR System thermocycler (Applied Biosystems; USA) with the following conditions: 95°C for 10 min, then 45 cycles of 95°C for 15 s and 60°C for 1 min.

    Article Title: Aberrant hypermethylation-mediated downregulation of antisense lncRNA ZNF667-AS1 and its sense gene ZNF667 correlate with progression and prognosis of esophageal squamous cell carcinoma
    Article Snippet: .. Quantitative real-time PCR was performed with Power SYBR Green PCR Master Mix (Life Technology, Foster City, CA, USA). .. Relative gene expression was determined by 2–ΔΔCT method normalized to GAPDH .

    Article Title: Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score
    Article Snippet: .. Most of RNA extractions and the cDNAs were done at Ankara University by the investigators from the participating countries. qRT-PCRs were done from plasmid DNAs of the target genes to generate standard curves with the Power SYBR® Green PCR Master Mix (Life Technologies, Applied Biosystems, Cat.No. ..

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: .. Semi-quantitative RT-PCR reaction was performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl Power SYBR Green PCR Master Mix and the cycling conditions; 50°C for 2 min, 95°C for 10 min; 40 cycles (95°C, 15 sec; 60°C, 1 min). .. The amplified products were separated on a 3% agarose gel and visualized by ethidium bromide staining.

    Article Title: The Role of Aberrant DNA Methylation in Misregulation of Gene Expression in Gonadotroph Nonfunctioning Pituitary Tumors
    Article Snippet: .. Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) was used for amplification. .. PCRs were run in 5-mL volumes containing 2.25 pmol of each primer on a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) in 384-well format.

    Article Title: Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
    Article Snippet: .. QPCR was performed by ViiA 7 sequence detection system using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and specific primers. .. Gene expression was assessed by the standard curve method and normalized to the reference gene beta-glucuronidase (GUSB).

    Article Title: Causative role of PDLIM2 epigenetic repression in lung cancer and therapeutic resistance
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) analysis The indicated tissues or cells were subjected to RNA extraction, RNA reverse transcription and real-time PCR using trizol, reverse transcriptase, and Power SYBR Green PCR Master Mix (Thermo Fisher Scientific, Waltham, MA USA) according to the product manufacture’s protocol , . .. Primer pairs used for qPCR were listed in Supplementary Table .

    Article Title: Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages
    Article Snippet: .. Transcripts were quantitated using Power SYBR® Green PCR Master Mix (Applied Biosystems, Carlsbad, CA, USA) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Article Title: Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia
    Article Snippet: .. The expression of EZH1 and other genes was measured using Power SYBR® Green PCR Master Mix (Applied Biosystems). .. Expression of all genes was determined by the comparative Ct method using ABL1 or GAPDH levels for normalization.

    Article Title: Elevated aldosterone and blood pressure in a mouse model of familial hyperaldosteronism with ClC-2 mutation
    Article Snippet: .. Gapdh (primers 5′-TGTAGACCATGTAGTTGAGGTCA-3′ and 5′-AGGTCGGTGTGAACGGATTTG-3′), Ren1 (5′-TCTGGGCACTCTTGTTGCTC-3′ and 5′-GGGGGAGGTAAGATTGGTCAA-3′), Slc13a2 (5′-CGAGAGTAATCCAGCAGTA-3′ and 5′-ATGAAGAGATTAACAAGAACAGAA-3′) and Sgk1 (5′-ATGCAGTAAACCAGCCGGT-3′ and 5′-TGATCCATCTTCGTACCCGT-3′) were amplified from kidney cDNA using Power SYBR Green PCR Master Mix (Applied Biosystems). .. Gene expression was evaluated relative to Gapdh , and mean ΔCT of WT controls and expressed as 2ΔΔCt (fold change).

    Isolation:

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: Paragraph title: Total RNA isolation, semi-quantitative RT-PCR of miRNAs and Quantitative RT-PCR ... PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix.

    Article Title: Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia
    Article Snippet: Paragraph title: RNA isolation and quantitative PCR (qPCR) ... The expression of EZH1 and other genes was measured using Power SYBR® Green PCR Master Mix (Applied Biosystems).

    Article Title: Elevated aldosterone and blood pressure in a mouse model of familial hyperaldosteronism with ClC-2 mutation
    Article Snippet: Quantitative real-time PCR Total RNA from adrenal glands and kidneys was isolated using the miRNeasy Mini Kit (Qiagen). .. Gapdh (primers 5′-TGTAGACCATGTAGTTGAGGTCA-3′ and 5′-AGGTCGGTGTGAACGGATTTG-3′), Ren1 (5′-TCTGGGCACTCTTGTTGCTC-3′ and 5′-GGGGGAGGTAAGATTGGTCAA-3′), Slc13a2 (5′-CGAGAGTAATCCAGCAGTA-3′ and 5′-ATGAAGAGATTAACAAGAACAGAA-3′) and Sgk1 (5′-ATGCAGTAAACCAGCCGGT-3′ and 5′-TGATCCATCTTCGTACCCGT-3′) were amplified from kidney cDNA using Power SYBR Green PCR Master Mix (Applied Biosystems).

    Size-exclusion Chromatography:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no. .. 4367659), and run using the 7900HT Fast Real-Time PCR System (ThermoFischer), with initial run for 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of denaturation at 95°C (15 sec) and amplification at 60°C (1 min).

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: Semi-quantitative RT-PCR reaction was performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl Power SYBR Green PCR Master Mix and the cycling conditions; 50°C for 2 min, 95°C for 10 min; 40 cycles (95°C, 15 sec; 60°C, 1 min). .. PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix.

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: .. Semi-quantitative RT-PCR reaction was performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl Power SYBR Green PCR Master Mix and the cycling conditions; 50°C for 2 min, 95°C for 10 min; 40 cycles (95°C, 15 sec; 60°C, 1 min). .. The amplified products were separated on a 3% agarose gel and visualized by ethidium bromide staining.

    Purification:

    Article Title: Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
    Article Snippet: For the gene expression analysis by qPCR, total RNA was purified from SAT samples using the miRNeasy Lipid Tissue Mini Kit (Qiagen, Germany). .. QPCR was performed by ViiA 7 sequence detection system using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and specific primers.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: Paragraph title: Total RNA isolation, semi-quantitative RT-PCR of miRNAs and Quantitative RT-PCR ... PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix.

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: .. Semi-quantitative RT-PCR reaction was performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl Power SYBR Green PCR Master Mix and the cycling conditions; 50°C for 2 min, 95°C for 10 min; 40 cycles (95°C, 15 sec; 60°C, 1 min). .. The amplified products were separated on a 3% agarose gel and visualized by ethidium bromide staining.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: .. Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no. .. 4367659), and run using the 7900HT Fast Real-Time PCR System (ThermoFischer), with initial run for 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of denaturation at 95°C (15 sec) and amplification at 60°C (1 min).

    Article Title: In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing
    Article Snippet: The cDNA synthesis reaction contained polyadenylated RNA from the previous step, 4 µL of 5× RT buffer, 1 µL of 0.1 M DTT, 1 µL of 10 mM dNTP mix, 1 µL of Ribonuclease Inhibitor and of SuperScript IV RT enzymes, and 1 µL of 100 µM universal stem-loop RT primer (5′-CTC ACA GTA CGT TGG TAT CCT TGT GAT GTT CGA TGC CAT ATT GTA CTG TGA GTT TTT TTT TVN-3′). .. Reactions were carried out in final volumes of 20 µL, consisting of 10 µL of Power SYBR Green PCR Master Mix (Life Technologies, USA), 0.25 µM of each primer (forward and reverse; Supplementary Table 1), 1 µL of cDNA template and 7 µL of PCR-grade water.

    Article Title: Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score
    Article Snippet: .. Most of RNA extractions and the cDNAs were done at Ankara University by the investigators from the participating countries. qRT-PCRs were done from plasmid DNAs of the target genes to generate standard curves with the Power SYBR® Green PCR Master Mix (Life Technologies, Applied Biosystems, Cat.No. ..

    Plasmid Preparation:

    Article Title: Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score
    Article Snippet: .. Most of RNA extractions and the cDNAs were done at Ankara University by the investigators from the participating countries. qRT-PCRs were done from plasmid DNAs of the target genes to generate standard curves with the Power SYBR® Green PCR Master Mix (Life Technologies, Applied Biosystems, Cat.No. ..

    SYBR Green Assay:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: .. Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no. .. 4367659), and run using the 7900HT Fast Real-Time PCR System (ThermoFischer), with initial run for 2 min at 50°C and 10 min at 95°C, followed by 40 cycles of denaturation at 95°C (15 sec) and amplification at 60°C (1 min).

    Article Title: Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
    Article Snippet: .. The BAX and Bcl2 primers as well as the reference gene primers were designed as previously described and added to a final concentration of 0.3 μM for each primer in a total volume of 10 μL containing Power SYBR Green PCR Master Mix (Applied Biosystems; Foster City, CA, USA), 1 μL of cDNA (1:10). .. The reactions were performed in triplicate in 384-well plates using QuantStudio 12 K Flex Thermal Cycler equipment (Applied Biosystems; Foster City, CA, USA).

    Article Title: Remodeling of the collagen matrix in aging skin promotes melanoma metastasis and affects immune cell motility
    Article Snippet: .. Each 20μl well reaction comprised of 10μl Power SYBR Green Master mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA. .. Standard curves were generated for all primers and each set of primers were normalized to 18 Primer pair (#AM1718, Ambion, Invitrogen).

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: .. PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix. .. PCR signals were detected using CFX Connect (Bio-Rad) and the cycling conditions; 50°C for 2 min, 95°C for 10 min; 40 cycles (95°C, 15 sec; 60°C, 1 min).

    Article Title: In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing
    Article Snippet: .. Reactions were carried out in final volumes of 20 µL, consisting of 10 µL of Power SYBR Green PCR Master Mix (Life Technologies, USA), 0.25 µM of each primer (forward and reverse; Supplementary Table 1), 1 µL of cDNA template and 7 µL of PCR-grade water. .. Amplification was performed with an ABI 7500 Real-time PCR System thermocycler (Applied Biosystems; USA) with the following conditions: 95°C for 10 min, then 45 cycles of 95°C for 15 s and 60°C for 1 min.

    Article Title: Aberrant hypermethylation-mediated downregulation of antisense lncRNA ZNF667-AS1 and its sense gene ZNF667 correlate with progression and prognosis of esophageal squamous cell carcinoma
    Article Snippet: .. Quantitative real-time PCR was performed with Power SYBR Green PCR Master Mix (Life Technology, Foster City, CA, USA). .. Relative gene expression was determined by 2–ΔΔCT method normalized to GAPDH .

    Article Title: Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score
    Article Snippet: .. Most of RNA extractions and the cDNAs were done at Ankara University by the investigators from the participating countries. qRT-PCRs were done from plasmid DNAs of the target genes to generate standard curves with the Power SYBR® Green PCR Master Mix (Life Technologies, Applied Biosystems, Cat.No. ..

    Article Title: Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis
    Article Snippet: .. Each 20μl reaction comprised 1μl Power SYBR Green Master Mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA. .. Standard curves were generated for each primer and used to perform relative quantification.

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: .. Semi-quantitative RT-PCR reaction was performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl Power SYBR Green PCR Master Mix and the cycling conditions; 50°C for 2 min, 95°C for 10 min; 40 cycles (95°C, 15 sec; 60°C, 1 min). .. The amplified products were separated on a 3% agarose gel and visualized by ethidium bromide staining.

    Article Title: The Role of Aberrant DNA Methylation in Misregulation of Gene Expression in Gonadotroph Nonfunctioning Pituitary Tumors
    Article Snippet: .. Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) was used for amplification. .. PCRs were run in 5-mL volumes containing 2.25 pmol of each primer on a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) in 384-well format.

    Article Title: Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
    Article Snippet: .. QPCR was performed by ViiA 7 sequence detection system using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and specific primers. .. Gene expression was assessed by the standard curve method and normalized to the reference gene beta-glucuronidase (GUSB).

    Article Title: Causative role of PDLIM2 epigenetic repression in lung cancer and therapeutic resistance
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) analysis The indicated tissues or cells were subjected to RNA extraction, RNA reverse transcription and real-time PCR using trizol, reverse transcriptase, and Power SYBR Green PCR Master Mix (Thermo Fisher Scientific, Waltham, MA USA) according to the product manufacture’s protocol , . .. Primer pairs used for qPCR were listed in Supplementary Table .

    Article Title: Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages
    Article Snippet: .. Transcripts were quantitated using Power SYBR® Green PCR Master Mix (Applied Biosystems, Carlsbad, CA, USA) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Article Title: Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia
    Article Snippet: .. The expression of EZH1 and other genes was measured using Power SYBR® Green PCR Master Mix (Applied Biosystems). .. Expression of all genes was determined by the comparative Ct method using ABL1 or GAPDH levels for normalization.

    Article Title: Elevated aldosterone and blood pressure in a mouse model of familial hyperaldosteronism with ClC-2 mutation
    Article Snippet: .. Gapdh (primers 5′-TGTAGACCATGTAGTTGAGGTCA-3′ and 5′-AGGTCGGTGTGAACGGATTTG-3′), Ren1 (5′-TCTGGGCACTCTTGTTGCTC-3′ and 5′-GGGGGAGGTAAGATTGGTCAA-3′), Slc13a2 (5′-CGAGAGTAATCCAGCAGTA-3′ and 5′-ATGAAGAGATTAACAAGAACAGAA-3′) and Sgk1 (5′-ATGCAGTAAACCAGCCGGT-3′ and 5′-TGATCCATCTTCGTACCCGT-3′) were amplified from kidney cDNA using Power SYBR Green PCR Master Mix (Applied Biosystems). .. Gene expression was evaluated relative to Gapdh , and mean ΔCT of WT controls and expressed as 2ΔΔCt (fold change).

    RNA Extraction:

    Article Title: IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Article Snippet: Paragraph title: RNA extraction, cDNA synthesis, and real-time PCR ... Real-time PCR was performed with Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, cat.no.

    Article Title: Aberrant hypermethylation-mediated downregulation of antisense lncRNA ZNF667-AS1 and its sense gene ZNF667 correlate with progression and prognosis of esophageal squamous cell carcinoma
    Article Snippet: Paragraph title: RNA extraction and quantitative real-time RT-PCR (qRT-PCR) assay ... Quantitative real-time PCR was performed with Power SYBR Green PCR Master Mix (Life Technology, Foster City, CA, USA).

    Article Title: Causative role of PDLIM2 epigenetic repression in lung cancer and therapeutic resistance
    Article Snippet: .. Quantitative polymerase chain reaction (qPCR) analysis The indicated tissues or cells were subjected to RNA extraction, RNA reverse transcription and real-time PCR using trizol, reverse transcriptase, and Power SYBR Green PCR Master Mix (Thermo Fisher Scientific, Waltham, MA USA) according to the product manufacture’s protocol , . .. Primer pairs used for qPCR were listed in Supplementary Table .

    Agarose Gel Electrophoresis:

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: The amplified products were separated on a 3% agarose gel and visualized by ethidium bromide staining. .. PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix.

    Incubation:

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: Following addition of 200 U SuperScript III (Invitrogen 18080051), 1 μl RNasin Plus RNase Inhibitor (Promega N2611), 1 μl 0.1 M DTT and 4 μl 5X RT buffer, the reaction was incubated at 50°C for 1 hour followed by 70°C for 15 minutes. .. PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix.

    Article Title: In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing
    Article Snippet: Reaction mixtures were incubated at 37°C for 30 min, followed by 95°C for 5 min to terminate the adenylation, and then transferred directly to ice. .. Reactions were carried out in final volumes of 20 µL, consisting of 10 µL of Power SYBR Green PCR Master Mix (Life Technologies, USA), 0.25 µM of each primer (forward and reverse; Supplementary Table 1), 1 µL of cDNA template and 7 µL of PCR-grade water.

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: Following addition of 200 U SuperScript III (Invitrogen 18080051), 1 μl RNasin Plus RNase Inhibitor (Promega N2611), 1 μl 0.1 M DTT and 4 μl 5X RT buffer, the reaction was incubated at 50°C for 1 hour followed by 70°C for 15 minutes. .. Semi-quantitative RT-PCR reaction was performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl Power SYBR Green PCR Master Mix and the cycling conditions; 50°C for 2 min, 95°C for 10 min; 40 cycles (95°C, 15 sec; 60°C, 1 min).

    Spectrophotometry:

    Article Title: Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
    Article Snippet: RNA concentration was measured using an ND-1000 spectrophotometer (Nanodrop, PeqLab). .. QPCR was performed by ViiA 7 sequence detection system using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and specific primers.

    Concentration Assay:

    Article Title: Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma
    Article Snippet: .. The BAX and Bcl2 primers as well as the reference gene primers were designed as previously described and added to a final concentration of 0.3 μM for each primer in a total volume of 10 μL containing Power SYBR Green PCR Master Mix (Applied Biosystems; Foster City, CA, USA), 1 μL of cDNA (1:10). .. The reactions were performed in triplicate in 384-well plates using QuantStudio 12 K Flex Thermal Cycler equipment (Applied Biosystems; Foster City, CA, USA).

    Article Title: Remodeling of the collagen matrix in aging skin promotes melanoma metastasis and affects immune cell motility
    Article Snippet: .. Each 20μl well reaction comprised of 10μl Power SYBR Green Master mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA. .. Standard curves were generated for all primers and each set of primers were normalized to 18 Primer pair (#AM1718, Ambion, Invitrogen).

    Article Title: Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score
    Article Snippet: Most of RNA extractions and the cDNAs were done at Ankara University by the investigators from the participating countries. qRT-PCRs were done from plasmid DNAs of the target genes to generate standard curves with the Power SYBR® Green PCR Master Mix (Life Technologies, Applied Biosystems, Cat.No. .. Formula; N = C p l a s m i d A · 1 , 07085.10 15 N: Copy number (copies/μl) C plasmid: Concentration of the plasmid DNA (μg/μl) A: Size of the plasmid with insert (bp)

    Article Title: Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis
    Article Snippet: .. Each 20μl reaction comprised 1μl Power SYBR Green Master Mix (4367659, Invitrogen), 1μl primer mix (Final concentration 0.5μM) and 1μl cDNA. .. Standard curves were generated for each primer and used to perform relative quantification.

    Article Title: Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
    Article Snippet: RNA concentration was measured using an ND-1000 spectrophotometer (Nanodrop, PeqLab). .. QPCR was performed by ViiA 7 sequence detection system using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and specific primers.

    CTG Assay:

    Article Title: In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing
    Article Snippet: The cDNA synthesis reaction contained polyadenylated RNA from the previous step, 4 µL of 5× RT buffer, 1 µL of 0.1 M DTT, 1 µL of 10 mM dNTP mix, 1 µL of Ribonuclease Inhibitor and of SuperScript IV RT enzymes, and 1 µL of 100 µM universal stem-loop RT primer (5′-CTC ACA GTA CGT TGG TAT CCT TGT GAT GTT CGA TGC CAT ATT GTA CTG TGA GTT TTT TTT TVN-3′). .. Reactions were carried out in final volumes of 20 µL, consisting of 10 µL of Power SYBR Green PCR Master Mix (Life Technologies, USA), 0.25 µM of each primer (forward and reverse; Supplementary Table 1), 1 µL of cDNA template and 7 µL of PCR-grade water.

    Staining:

    Article Title: Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions
    Article Snippet: The amplified products were separated on a 3% agarose gel and visualized by ethidium bromide staining. .. PCR reaction were performed with Power SYB Green PCR Master Mix (Applied Biosystems 4367659) in a 20 μl reaction containing 5 pmol forward and reverse primer, 0.1 μl cDNA template and 10 μl of Power SYBR Green PCR Master Mix.

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