power sybr green pcr master mix kit  (Thermo Fisher)


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    Name:
    Power SYBR Green PCR Master Mix
    Description:
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of Power SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis Get superior nucleic acid quantitation with Applied Biosystems Power SYBR Green Master Mix Power SYBR Master Mix offers superior sensitivity and reproducibility detecting as few as two copies of a target gene over a broad range of template concentrations • Everything you need for SYBR Green dye based PCR amplification and detection in a convenient single tube format Power SYBR Green PCR Master Mix contains all of the components excluding the template and primers for superior SYBR Green reagent based real time PCR• The optimized formulation contains highly purified AmpliTaq Gold DNA Polymerase LD to offer greater sensitivity than classic SYBR Green PCR master mix• Power SYBR Green PCR Master Mix contains a blend of dTTP dUTP that is compatible with AmpErase UNG to minimize carryover contamination• Includes a proprietary version of ROX dye an internal passive reference to normalize non PCR related fluorescence fluctuations to minimize well to well variability that result from a variety of causes such as pipetting error and sample evaporation• Power SYBR Green reagent based PCR chemistry easily replaces the existing SYBR Green PCR Master Mix in Applied Biosystems protocols using the same setup and thermal cycling conditions
    Catalog Number:
    4367659
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    Applications:
    Enzymes & Master Mixes for Real-Time PCR|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Gene Expression Analysis & Genotyping
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    Structured Review

    Thermo Fisher power sybr green pcr master mix kit
    Alternative Product Try PowerUp SYBR Green Master Mix our newest high performance SYBR dye based master mix for superior performance at a very competitive price With PowerUp SYBR Green Master Mix we ve taken the best of Power SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis Get superior nucleic acid quantitation with Applied Biosystems Power SYBR Green Master Mix Power SYBR Master Mix offers superior sensitivity and reproducibility detecting as few as two copies of a target gene over a broad range of template concentrations • Everything you need for SYBR Green dye based PCR amplification and detection in a convenient single tube format Power SYBR Green PCR Master Mix contains all of the components excluding the template and primers for superior SYBR Green reagent based real time PCR• The optimized formulation contains highly purified AmpliTaq Gold DNA Polymerase LD to offer greater sensitivity than classic SYBR Green PCR master mix• Power SYBR Green PCR Master Mix contains a blend of dTTP dUTP that is compatible with AmpErase UNG to minimize carryover contamination• Includes a proprietary version of ROX dye an internal passive reference to normalize non PCR related fluorescence fluctuations to minimize well to well variability that result from a variety of causes such as pipetting error and sample evaporation• Power SYBR Green reagent based PCR chemistry easily replaces the existing SYBR Green PCR Master Mix in Applied Biosystems protocols using the same setup and thermal cycling conditions
    https://www.bioz.com/result/power sybr green pcr master mix kit/product/Thermo Fisher
    Average 99 stars, based on 220 article reviews
    Price from $9.99 to $1999.99
    power sybr green pcr master mix kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling
    Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Power SYBR Green PCR Master Mix (Life Technologies, Gaitherburg, MD). .. The reactions were performed using the 7300 Real-Time PCR System instrument (Applied Biosystems, Foster City, CA).

    Article Title: CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells
    Article Snippet: .. Equal volumes of prepared cDNA were used for quantification of HSV-1 gene ICP0 and ICP4 transcripts with the Power SYBR Green PCR master mix (Applied biosystems) according to the manufacturer's protocol. ..

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis
    Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system. .. Quantification was carried out relative to HEK293 cells, and values were normalized to HPRT expression.

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells
    Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Power SYBR Green PCR (Applied Biosystems by Life Technologies, Warrington, UK) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Article Title: Estradiol Protects Proopiomelanocortin Neurons Against Insulin Resistance
    Article Snippet: .. The results were as follows: Pik3cb (p110 β ), m = –3.481, r 2 = 0.95, efficiency = 96%; Gapdh , m = –3.35, r 2 = 0.99, efficiency = 98.7%; Socs3 , m = –3.294, r 2 = 0.90, efficiency = 100%; Trpc5 , m = –3.161, r 2 = 0.95, efficiency = 100%; Stim1 , m = –3.407, r 2 = 0.98, efficiency = 97%. qPCR was performed on a Quantstudio 7 Flex Real-Time PCR System (Life Technologies, Grand Island, NY) using Power SYBR Green Master Mix (Life Technologies) according to established protocols ( ). ..

    Article Title: secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus. secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus
    Article Snippet: .. 2.5 Quantitative real‐time PCR (qRT‐PCR) Power SYBR Green PCR Master Mix (Applied Biosystems, Carlsbad, California, USA) was used to assay the expression of genes by qRT‐PCR according to the manufacturer's instructions. .. 16s RNA was used to normalize the expression levels.

    Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition
    Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Power SYBR Green Master Mix (Applied Biosystems) with the HT7900 Fast Real Time PCR machine (Applied Biosystems). .. Standard curves were generated using serial dilutions of cDNA with arbitrary copy numbers.

    Amplification:

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis
    Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system. .. Quantification was carried out relative to HEK293 cells, and values were normalized to HPRT expression.

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Synthesized:

    Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling
    Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Power SYBR Green PCR Master Mix (Life Technologies, Gaitherburg, MD). .. The reactions were performed using the 7300 Real-Time PCR System instrument (Applied Biosystems, Foster City, CA).

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Quantitative RT-PCR:

    Article Title: secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus. secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus
    Article Snippet: .. 2.5 Quantitative real‐time PCR (qRT‐PCR) Power SYBR Green PCR Master Mix (Applied Biosystems, Carlsbad, California, USA) was used to assay the expression of genes by qRT‐PCR according to the manufacturer's instructions. .. 16s RNA was used to normalize the expression levels.

    Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition
    Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Power SYBR Green Master Mix (Applied Biosystems) with the HT7900 Fast Real Time PCR machine (Applied Biosystems). .. Standard curves were generated using serial dilutions of cDNA with arbitrary copy numbers.

    Real-time Polymerase Chain Reaction:

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis
    Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system. .. Quantification was carried out relative to HEK293 cells, and values were normalized to HPRT expression.

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells
    Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Power SYBR Green PCR (Applied Biosystems by Life Technologies, Warrington, UK) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Article Title: Estradiol Protects Proopiomelanocortin Neurons Against Insulin Resistance
    Article Snippet: .. The results were as follows: Pik3cb (p110 β ), m = –3.481, r 2 = 0.95, efficiency = 96%; Gapdh , m = –3.35, r 2 = 0.99, efficiency = 98.7%; Socs3 , m = –3.294, r 2 = 0.90, efficiency = 100%; Trpc5 , m = –3.161, r 2 = 0.95, efficiency = 100%; Stim1 , m = –3.407, r 2 = 0.98, efficiency = 97%. qPCR was performed on a Quantstudio 7 Flex Real-Time PCR System (Life Technologies, Grand Island, NY) using Power SYBR Green Master Mix (Life Technologies) according to established protocols ( ). ..

    Article Title: secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus. secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus
    Article Snippet: .. 2.5 Quantitative real‐time PCR (qRT‐PCR) Power SYBR Green PCR Master Mix (Applied Biosystems, Carlsbad, California, USA) was used to assay the expression of genes by qRT‐PCR according to the manufacturer's instructions. .. 16s RNA was used to normalize the expression levels.

    Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition
    Article Snippet: .. For quantitative RT-PCR (qRT-PCR), reverse-transcribed cDNA was used for real-time PCR using Power SYBR Green Master Mix (Applied Biosystems) with the HT7900 Fast Real Time PCR machine (Applied Biosystems). .. Standard curves were generated using serial dilutions of cDNA with arbitrary copy numbers.

    Polymerase Chain Reaction:

    Article Title: Cyclophilin A (CypA) Interacts with NF-κB Subunit, p65/RelA, and Contributes to NF-κB Activation Signaling
    Article Snippet: .. Using the cDNA synthesized from the SuperScript III First-Strand Synthesis System as templates, individual specific primers (p65 F, 5′-GACGACTGTTCCCCCTC-3′ and R, 5′-CCTCGCACTTGTAGCGG-3′ ; p50 F, 5′-GCAAACCTGGGAATACTTCATGTGACTAAG-3′ and R, 5′-ATAGGCAA-CCTCAGAATGCACCAGAAGTCC-3′ ; IL-1β F, 5′- TGCGTGTTGAAAGATGATAA-G-3′ and R, 5′- TTGGGGAACTGGGCAGAC-3′ ; IL-6 F, 5′-CTCCAGAACAGATTT-GAGAGTAGTG-3′ and R, 5′-TTGTGGTTGGGTCAGGGGTG-3′ ; IL-8 F, 5′-GGGTT-GTGGAGAAGTTTTTG-3′ and R, 5′-GTTTCACTGGCATCTTCACTG-3′ ) were added to each template and mixed with the Power SYBR Green PCR Master Mix (Life Technologies, Gaitherburg, MD). .. The reactions were performed using the 7300 Real-Time PCR System instrument (Applied Biosystems, Foster City, CA).

    Article Title: CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells
    Article Snippet: .. Equal volumes of prepared cDNA were used for quantification of HSV-1 gene ICP0 and ICP4 transcripts with the Power SYBR Green PCR master mix (Applied biosystems) according to the manufacturer's protocol. ..

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis
    Article Snippet: .. Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system. .. Quantification was carried out relative to HEK293 cells, and values were normalized to HPRT expression.

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

    Article Title: Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells
    Article Snippet: .. For mRNA analysis, total RNA was extracted from cells by TRIzol (Invitrogen) according to the manufacturer’s protocol and reverse-transcribed into cDNA using the QuantiTect® reverse transcription kit. cDNA was quantitated using Power SYBR Green PCR (Applied Biosystems by Life Technologies, Warrington, UK) and the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Article Title: secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus. secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus
    Article Snippet: .. 2.5 Quantitative real‐time PCR (qRT‐PCR) Power SYBR Green PCR Master Mix (Applied Biosystems, Carlsbad, California, USA) was used to assay the expression of genes by qRT‐PCR according to the manufacturer's instructions. .. 16s RNA was used to normalize the expression levels.

    Expressing:

    Article Title: secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus. secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus
    Article Snippet: .. 2.5 Quantitative real‐time PCR (qRT‐PCR) Power SYBR Green PCR Master Mix (Applied Biosystems, Carlsbad, California, USA) was used to assay the expression of genes by qRT‐PCR according to the manufacturer's instructions. .. 16s RNA was used to normalize the expression levels.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients
    Article Snippet: .. First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts. .. GAPDH mRNA was used as the housekeeping marker.

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    Thermo Fisher power sybr green pcr master mix
    Hypermutation of the FeLV-B genome and APOBEC3G mRNA expression in human cells. FeLV genomic sequences (segments of Gag and Pol) were cloned by <t>PCR</t> from infected human cells, and individual templates were sequenced and compared to the reference input virus (pFGB clone). APOBEC3G mRNA expression was determined in the same cells (prior to infection) by quantitative real-time PCR (with <t>SYBR</t> green). (A) Representative plots of hypermutation visualized by the online HYPERMUT program ( www.hiv.lanl.gov/content/sequence/HYPERMUT/hypermut.html ), where sequence changes relative to the reference FeLV-B genome are color coded (red, GG→AG; cyan, GA→AA; green, GC→AC; magenta, GT→AT; black, non-G→A). (B) X/Y plots of G→A mutation (per kilobase) against APOBEC3G mRNA levels (where the level in HEK293 cells is taken as 1) with cell lines sorted according to FeLV restriction phenotype. Results for LCLs, which are discordant by virtue of their low levels of infectious virion release ( Table 2 ) but postinfection accumulation of proviral DNA ( Fig. 3 ), are enclosed by a dashed oval. (C) Percentage of G→A mutations that conform to the A3G signature ( 42 ) for all cell lines in which significant levels of mutations were detected. Blue-gray bars, hematopoietic cells; yellow bars, nonhematopoietic cells. (D) Relative levels of APOBEC3G mRNA (on a log 10 scale, with the level in HEK293 cells taken as 1) for all the cell lines tested, sorted into hematopoietic (blue circles) and nonhematopoietic (green circles) cell lines. Nonpermissive cells (nonspreading, with low virion release) are represented by black circles.
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8809 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/power sybr green pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 8809 article reviews
    Price from $9.99 to $1999.99
    power sybr green pcr master mix - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher power sybr green rna to ct rt qpcr kit
    COS hiPSC cohort reprogramming and differentiation. a Validated hiPSCs (from 14 individuals with childhood-onset-schizophrenia (COS) and 12 unrelated healthy controls) and NPCs (12 COS; 12 control individuals) yielded 94 <t>RNA-Seq</t> samples (11 COS; 11 control individuals). b Schematic illustration of the reprogramming and differentiation process, noting the yield at each stage. c Sex breakdown of the COS-control cohort. d Breakdown of SZ-associated copy number variants in the 11 COS patients with RNA-Seq data. e Representative <t>qPCR</t> validation of NANOG, NESTIN , and SYN1 expression in hiPSCs (white bar), NPCs (light gray) and 6-week-old neurons (dark gray) from three individuals. f FACS analysis for pluripotency markers TRA-1-60 (left) and SSEA4 (right) in representative control (blue, n = 17) and COS (red, n = 16) hiPSCs. g FACS analysis for NPC markers SOX2 (left) and NESTIN (right) in control (blue, n = 34) and COS (red, n = 37) NPCs. h Representative images of NPCs (left) and 6-week-old forebrain neurons (right) from control (top) and COS (bottom). NPCs stained with SOX2 (red) and NESTIN (green); neurons stained with MAP2 (red). DAPI-stained nuclei (blue). Scale bar=50 μm. i Computational workflow showing quality control, integration with external data sets, computational deconvolution with Cibersort, decomposition multiple sources of expression variation with variancePartition, coexpression analysis with WGCNA, differential expression and concordance analysis
    Power Sybr Green Rna To Ct Rt Qpcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/power sybr green rna to ct rt qpcr kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    power sybr green rna to ct rt qpcr kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    Hypermutation of the FeLV-B genome and APOBEC3G mRNA expression in human cells. FeLV genomic sequences (segments of Gag and Pol) were cloned by PCR from infected human cells, and individual templates were sequenced and compared to the reference input virus (pFGB clone). APOBEC3G mRNA expression was determined in the same cells (prior to infection) by quantitative real-time PCR (with SYBR green). (A) Representative plots of hypermutation visualized by the online HYPERMUT program ( www.hiv.lanl.gov/content/sequence/HYPERMUT/hypermut.html ), where sequence changes relative to the reference FeLV-B genome are color coded (red, GG→AG; cyan, GA→AA; green, GC→AC; magenta, GT→AT; black, non-G→A). (B) X/Y plots of G→A mutation (per kilobase) against APOBEC3G mRNA levels (where the level in HEK293 cells is taken as 1) with cell lines sorted according to FeLV restriction phenotype. Results for LCLs, which are discordant by virtue of their low levels of infectious virion release ( Table 2 ) but postinfection accumulation of proviral DNA ( Fig. 3 ), are enclosed by a dashed oval. (C) Percentage of G→A mutations that conform to the A3G signature ( 42 ) for all cell lines in which significant levels of mutations were detected. Blue-gray bars, hematopoietic cells; yellow bars, nonhematopoietic cells. (D) Relative levels of APOBEC3G mRNA (on a log 10 scale, with the level in HEK293 cells taken as 1) for all the cell lines tested, sorted into hematopoietic (blue circles) and nonhematopoietic (green circles) cell lines. Nonpermissive cells (nonspreading, with low virion release) are represented by black circles.

    Journal: Journal of Virology

    Article Title: Barriers to Infection of Human Cells by Feline Leukemia Virus: Insights into Resistance to Zoonosis

    doi: 10.1128/JVI.02119-16

    Figure Lengend Snippet: Hypermutation of the FeLV-B genome and APOBEC3G mRNA expression in human cells. FeLV genomic sequences (segments of Gag and Pol) were cloned by PCR from infected human cells, and individual templates were sequenced and compared to the reference input virus (pFGB clone). APOBEC3G mRNA expression was determined in the same cells (prior to infection) by quantitative real-time PCR (with SYBR green). (A) Representative plots of hypermutation visualized by the online HYPERMUT program ( www.hiv.lanl.gov/content/sequence/HYPERMUT/hypermut.html ), where sequence changes relative to the reference FeLV-B genome are color coded (red, GG→AG; cyan, GA→AA; green, GC→AC; magenta, GT→AT; black, non-G→A). (B) X/Y plots of G→A mutation (per kilobase) against APOBEC3G mRNA levels (where the level in HEK293 cells is taken as 1) with cell lines sorted according to FeLV restriction phenotype. Results for LCLs, which are discordant by virtue of their low levels of infectious virion release ( Table 2 ) but postinfection accumulation of proviral DNA ( Fig. 3 ), are enclosed by a dashed oval. (C) Percentage of G→A mutations that conform to the A3G signature ( 42 ) for all cell lines in which significant levels of mutations were detected. Blue-gray bars, hematopoietic cells; yellow bars, nonhematopoietic cells. (D) Relative levels of APOBEC3G mRNA (on a log 10 scale, with the level in HEK293 cells taken as 1) for all the cell lines tested, sorted into hematopoietic (blue circles) and nonhematopoietic (green circles) cell lines. Nonpermissive cells (nonspreading, with low virion release) are represented by black circles.

    Article Snippet: Aliquots (12.5 ng) of cDNA were amplified in triplicate using Power SYBR green PCR master mix (Thermo Fisher Scientific) and Qiagen QuantiTect primer assays for human hypoxanthine phosphoribosyltransferase (HPRT) (QT00059066), APOBEC3G (QT00070770), SLC20A1 (PIT-1) (QT00028763), or SLC19A2 (THTR1) (QT00007847) on an ABI 7500 real-time PCR system.

    Techniques: Expressing, Genomic Sequencing, Clone Assay, Polymerase Chain Reaction, Infection, Real-time Polymerase Chain Reaction, SYBR Green Assay, Sequencing, Mutagenesis

    The thymus produces a significant number of Pth transcripts that increase after TPTX. (A) The thymus produced Pth transcripts. Mice in which serum PTH levels recovered (n = 10) were euthanized, and the heart, lung, liver, spleen, kidney, pancreas, testis or ovary, hypothalamus, pituitary gland, thymus, and adrenal glands were harvested. RNA qRT-PCR was performed on the tissue samples using SYBR Green/Taqman Master Mix with custom primers for the mouse Pth transcript and a commercial probe-set for 18S rRNA (endogenous control). Expression data were normalized to 18S rRNA, and fold changes of expression against arbitrary numbers were calculated using the 2 − ΔΔ Ct ). (B) Pth transcripts increased after TPTX. Thymi from unoperated mice (control) were harvested, and the same procedures were performed as in (A). qRT-PCR was performed as described with a commercial probe (gray) and custom probes (black). Student t test was performed. ** P

    Journal: Endocrinology

    Article Title: Thymic PTH Increases After Thyroparathyroidectomy in C57BL/KaLwRij Mice

    doi: 10.1210/en.2017-03083

    Figure Lengend Snippet: The thymus produces a significant number of Pth transcripts that increase after TPTX. (A) The thymus produced Pth transcripts. Mice in which serum PTH levels recovered (n = 10) were euthanized, and the heart, lung, liver, spleen, kidney, pancreas, testis or ovary, hypothalamus, pituitary gland, thymus, and adrenal glands were harvested. RNA qRT-PCR was performed on the tissue samples using SYBR Green/Taqman Master Mix with custom primers for the mouse Pth transcript and a commercial probe-set for 18S rRNA (endogenous control). Expression data were normalized to 18S rRNA, and fold changes of expression against arbitrary numbers were calculated using the 2 − ΔΔ Ct ). (B) Pth transcripts increased after TPTX. Thymi from unoperated mice (control) were harvested, and the same procedures were performed as in (A). qRT-PCR was performed as described with a commercial probe (gray) and custom probes (black). Student t test was performed. ** P

    Article Snippet: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed with the Power SYBR Green PCR Master Mix on the Step-One-Plus system (Thermo Fisher Scientific).

    Techniques: Produced, Mouse Assay, Quantitative RT-PCR, SYBR Green Assay, Expressing

    Accumulation of immunostimulatory 5′-ppp-vtRNAs during lytic reactivation. a Predicted secondary structure of vtRNAs generated by RNAfold. b SYBR-Gold staining of in vitro transcribed vtRNAs with or without CIP treatment. c HCT116 ISG54-luciferase reporter cells were transfected with 100 ng in vitro transcribed vtRNAs with or without CIP treatment. Cells were harvested 24 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. d BC-3 cells were reactivated for 3 days and expression of DUSP11 was quantified by RT-qPCR. L latency, D1–D3 lytic reactivation for 1 day to 3 days. The DUSP11 expression was normalized to the level of 18S rRNA and L was set as 1. e Cell lysates were prepared from BC-3 cells described in ( d ) and DUSP11 protein levels were monitored by Western blot. GAPDH was run as a loading control. f Latent and lytic BC-3 cells were subjected to RNAP II ChIP-qPCR analysis. Signals were normalized to input. g ). h HCT116 ISG54-luciferase reporter cells were transfected with vtRNA or U1 RNA isolated by antisense oligonucleotide affinity selection from either latent or lytic BC-3 cells. Cells were harvested 12 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. Error bars in all panels represent mean ± SD from three independent experiments. p Values were determined by the Student’s t test, * p

    Journal: Nature Communications

    Article Title: RIG-I like receptor sensing of host RNAs facilitates the cell-intrinsic immune response to KSHV infection

    doi: 10.1038/s41467-018-07314-7

    Figure Lengend Snippet: Accumulation of immunostimulatory 5′-ppp-vtRNAs during lytic reactivation. a Predicted secondary structure of vtRNAs generated by RNAfold. b SYBR-Gold staining of in vitro transcribed vtRNAs with or without CIP treatment. c HCT116 ISG54-luciferase reporter cells were transfected with 100 ng in vitro transcribed vtRNAs with or without CIP treatment. Cells were harvested 24 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. d BC-3 cells were reactivated for 3 days and expression of DUSP11 was quantified by RT-qPCR. L latency, D1–D3 lytic reactivation for 1 day to 3 days. The DUSP11 expression was normalized to the level of 18S rRNA and L was set as 1. e Cell lysates were prepared from BC-3 cells described in ( d ) and DUSP11 protein levels were monitored by Western blot. GAPDH was run as a loading control. f Latent and lytic BC-3 cells were subjected to RNAP II ChIP-qPCR analysis. Signals were normalized to input. g ). h HCT116 ISG54-luciferase reporter cells were transfected with vtRNA or U1 RNA isolated by antisense oligonucleotide affinity selection from either latent or lytic BC-3 cells. Cells were harvested 12 h posttransfection and subjected to luciferase assay. Mock indicated cells without RNA transfection and was set as 1. Error bars in all panels represent mean ± SD from three independent experiments. p Values were determined by the Student’s t test, * p

    Article Snippet: RNA was DNase I (NEB) treated at 37 °C for 20 min, and inactivated with EDTA at 70 °C for 10 min. cDNA was synthesized from DNase-treated RNA with random 9-mer (Integrated DNA Technologies) and M-MLV RT (Promega). qPCR was performed using the PowerUp SYBR Green qPCR kit (Thermo Scientific) with appropriate primers (Supplementary Table ).

    Techniques: Generated, Staining, In Vitro, Luciferase, Transfection, Expressing, Quantitative RT-PCR, Western Blot, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Isolation, Selection

    COS hiPSC cohort reprogramming and differentiation. a Validated hiPSCs (from 14 individuals with childhood-onset-schizophrenia (COS) and 12 unrelated healthy controls) and NPCs (12 COS; 12 control individuals) yielded 94 RNA-Seq samples (11 COS; 11 control individuals). b Schematic illustration of the reprogramming and differentiation process, noting the yield at each stage. c Sex breakdown of the COS-control cohort. d Breakdown of SZ-associated copy number variants in the 11 COS patients with RNA-Seq data. e Representative qPCR validation of NANOG, NESTIN , and SYN1 expression in hiPSCs (white bar), NPCs (light gray) and 6-week-old neurons (dark gray) from three individuals. f FACS analysis for pluripotency markers TRA-1-60 (left) and SSEA4 (right) in representative control (blue, n = 17) and COS (red, n = 16) hiPSCs. g FACS analysis for NPC markers SOX2 (left) and NESTIN (right) in control (blue, n = 34) and COS (red, n = 37) NPCs. h Representative images of NPCs (left) and 6-week-old forebrain neurons (right) from control (top) and COS (bottom). NPCs stained with SOX2 (red) and NESTIN (green); neurons stained with MAP2 (red). DAPI-stained nuclei (blue). Scale bar=50 μm. i Computational workflow showing quality control, integration with external data sets, computational deconvolution with Cibersort, decomposition multiple sources of expression variation with variancePartition, coexpression analysis with WGCNA, differential expression and concordance analysis

    Journal: Nature Communications

    Article Title: Transcriptional signatures of schizophrenia in hiPSC-derived NPCs and neurons are concordant with post-mortem adult brains

    doi: 10.1038/s41467-017-02330-5

    Figure Lengend Snippet: COS hiPSC cohort reprogramming and differentiation. a Validated hiPSCs (from 14 individuals with childhood-onset-schizophrenia (COS) and 12 unrelated healthy controls) and NPCs (12 COS; 12 control individuals) yielded 94 RNA-Seq samples (11 COS; 11 control individuals). b Schematic illustration of the reprogramming and differentiation process, noting the yield at each stage. c Sex breakdown of the COS-control cohort. d Breakdown of SZ-associated copy number variants in the 11 COS patients with RNA-Seq data. e Representative qPCR validation of NANOG, NESTIN , and SYN1 expression in hiPSCs (white bar), NPCs (light gray) and 6-week-old neurons (dark gray) from three individuals. f FACS analysis for pluripotency markers TRA-1-60 (left) and SSEA4 (right) in representative control (blue, n = 17) and COS (red, n = 16) hiPSCs. g FACS analysis for NPC markers SOX2 (left) and NESTIN (right) in control (blue, n = 34) and COS (red, n = 37) NPCs. h Representative images of NPCs (left) and 6-week-old forebrain neurons (right) from control (top) and COS (bottom). NPCs stained with SOX2 (red) and NESTIN (green); neurons stained with MAP2 (red). DAPI-stained nuclei (blue). Scale bar=50 μm. i Computational workflow showing quality control, integration with external data sets, computational deconvolution with Cibersort, decomposition multiple sources of expression variation with variancePartition, coexpression analysis with WGCNA, differential expression and concordance analysis

    Article Snippet: Transcript analysis was carried out using a QuantStudio™ 7 Flex Real-Time PCR System using the Power SYBR green RNA-to-Ct RT-qPCR kit for primers (all ThermoFisher Scientific).

    Techniques: RNA Sequencing Assay, Real-time Polymerase Chain Reaction, Expressing, FACS, Staining