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Difco potato dextrose agar
Potato Dextrose Agar, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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potato dextrose agar - by Bioz Stars, 2022-01
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  • pda  (Difco)
    86
    Difco pda
    NDR kinase CoCbk1 plays an essential role for cell morphogenesis of infection structures and pathogenesis in C . <t>orbiculare</t> . (A) Schematic illustration of CoCbk1 analog-sensitive (AS) protein and sequence alignment including the ATP-binding pocket in C . orbiculare (Co) Cbk1 with corresponding sequences of S . cerevisiae (Sc) Cbk1. The kinase domain and site of the amino acid exchange (M352A) are marked. (B) Colonies of CoCbk1-AS strains on <t>PDA</t> and PDA supplemented with 0.5 μM 1NA-PP1. WT, wild type strain 104-T; Cbk1-AS1 and Cbk1-AS3, CoCbk1 analog-sensitive strain in WT background. (C) Mean percentage (±SE) of conidial germination, normal appressoria and penetration hyphae of CoCbk1-AS strains on cellulose membranes. Cells on a cellophane membrane were treated with 0.5 μM 1NA-PP1 at the indicated times. Cells were observed at 48 h after inoculation. At least 300 appressoria on a membrane were observed in each of three independent experiments. Values are means of three replications. Control, incubated in distilled water for 48 h. (D) Development of infection structures of CoCbk1-AS strains on cellulose membranes treated with 0.5 μM 1NA-PP1 at indicated times after start of incubation. Control, incubated in distilled water for 48 h; Co, conidium; Ap, appressorium; Ph, penetration hyphae. Scale bar, 10 μm. (E) Pathogenicity assay of CoCbk1-AS strains on intact cucumber cotyledons after 7 d at 24°C. Conidial suspensions of indicated strains were prepared in distilled water or in distilled water containing 0.5 μM 1NA-PP1 and dropped onto detached cucumber cotyledons. (F) Development of infection structures of CoCbk1-AS strains on lower surface of detached cucumber cotyledons treated with 0.5 μM 1NA-PP1 at indicated times after start of incubation. Cells were observed at 3 d after inoculation. Control, incubated in distilled water for 3 d; Scale bar, 10 μm. Penetration hyphae were stained with lactophenol aniline blue. (G) Mean percentage (±SE) of normal appressoria and penetration hyphae of CoCbk1-AS strains on lower surface of detached cucumber cotyledons. Cells were treated with 0.5 μM 1NA-PP1 at the indicated times and observed at 3 d after inoculation. At least 300 appressoria on three cotyledons were observed in each of three independent experiments. Values are means of three replications. Control, incubated in distilled water for 3 d.
    Pda, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pda/product/Difco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pda - by Bioz Stars, 2022-01
    86/100 stars
      Buy from Supplier

    86
    Difco potato dextrose agar pda
    TSA enhances the antibiotic activity of T . atroviride mycelium-free culture filtrates (MFCF) against R . solani . T . atroviride was grown in <t>PDB</t> amended or not with 300 nM TSA, incubated for 7 days at 28 °C. The MFCFs were added to <t>PDA</t> 1× medium at a final concentration of 60%. R . solani radial growth was measured at 12, 24, 36, 48, and 60 h post-inoculation. R . solani growth on PDA (black bars), PDA plates amended with 300 nM TSA (arrow filled bars), PDA plates amended with 60% of T . atroviride MFCF (crosshatched bars), and PDA plates amended with 60% of T . atroviride MFCF grown in PDB plus TSA (black dotted bars) are shown. The bars show the mean ± SD of three independent biological replicates. Different letters are used to indicate means that differ significantly ( P
    Potato Dextrose Agar Pda, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/potato dextrose agar pda/product/Difco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    potato dextrose agar pda - by Bioz Stars, 2022-01
    86/100 stars
      Buy from Supplier

    Image Search Results


    NDR kinase CoCbk1 plays an essential role for cell morphogenesis of infection structures and pathogenesis in C . orbiculare . (A) Schematic illustration of CoCbk1 analog-sensitive (AS) protein and sequence alignment including the ATP-binding pocket in C . orbiculare (Co) Cbk1 with corresponding sequences of S . cerevisiae (Sc) Cbk1. The kinase domain and site of the amino acid exchange (M352A) are marked. (B) Colonies of CoCbk1-AS strains on PDA and PDA supplemented with 0.5 μM 1NA-PP1. WT, wild type strain 104-T; Cbk1-AS1 and Cbk1-AS3, CoCbk1 analog-sensitive strain in WT background. (C) Mean percentage (±SE) of conidial germination, normal appressoria and penetration hyphae of CoCbk1-AS strains on cellulose membranes. Cells on a cellophane membrane were treated with 0.5 μM 1NA-PP1 at the indicated times. Cells were observed at 48 h after inoculation. At least 300 appressoria on a membrane were observed in each of three independent experiments. Values are means of three replications. Control, incubated in distilled water for 48 h. (D) Development of infection structures of CoCbk1-AS strains on cellulose membranes treated with 0.5 μM 1NA-PP1 at indicated times after start of incubation. Control, incubated in distilled water for 48 h; Co, conidium; Ap, appressorium; Ph, penetration hyphae. Scale bar, 10 μm. (E) Pathogenicity assay of CoCbk1-AS strains on intact cucumber cotyledons after 7 d at 24°C. Conidial suspensions of indicated strains were prepared in distilled water or in distilled water containing 0.5 μM 1NA-PP1 and dropped onto detached cucumber cotyledons. (F) Development of infection structures of CoCbk1-AS strains on lower surface of detached cucumber cotyledons treated with 0.5 μM 1NA-PP1 at indicated times after start of incubation. Cells were observed at 3 d after inoculation. Control, incubated in distilled water for 3 d; Scale bar, 10 μm. Penetration hyphae were stained with lactophenol aniline blue. (G) Mean percentage (±SE) of normal appressoria and penetration hyphae of CoCbk1-AS strains on lower surface of detached cucumber cotyledons. Cells were treated with 0.5 μM 1NA-PP1 at the indicated times and observed at 3 d after inoculation. At least 300 appressoria on three cotyledons were observed in each of three independent experiments. Values are means of three replications. Control, incubated in distilled water for 3 d.

    Journal: PLoS Pathogens

    Article Title: The morphogenesis-related NDR kinase pathway of Colletotrichum orbiculare is required for translating plant surface signals into infection-related morphogenesis and pathogenesis

    doi: 10.1371/journal.ppat.1006189

    Figure Lengend Snippet: NDR kinase CoCbk1 plays an essential role for cell morphogenesis of infection structures and pathogenesis in C . orbiculare . (A) Schematic illustration of CoCbk1 analog-sensitive (AS) protein and sequence alignment including the ATP-binding pocket in C . orbiculare (Co) Cbk1 with corresponding sequences of S . cerevisiae (Sc) Cbk1. The kinase domain and site of the amino acid exchange (M352A) are marked. (B) Colonies of CoCbk1-AS strains on PDA and PDA supplemented with 0.5 μM 1NA-PP1. WT, wild type strain 104-T; Cbk1-AS1 and Cbk1-AS3, CoCbk1 analog-sensitive strain in WT background. (C) Mean percentage (±SE) of conidial germination, normal appressoria and penetration hyphae of CoCbk1-AS strains on cellulose membranes. Cells on a cellophane membrane were treated with 0.5 μM 1NA-PP1 at the indicated times. Cells were observed at 48 h after inoculation. At least 300 appressoria on a membrane were observed in each of three independent experiments. Values are means of three replications. Control, incubated in distilled water for 48 h. (D) Development of infection structures of CoCbk1-AS strains on cellulose membranes treated with 0.5 μM 1NA-PP1 at indicated times after start of incubation. Control, incubated in distilled water for 48 h; Co, conidium; Ap, appressorium; Ph, penetration hyphae. Scale bar, 10 μm. (E) Pathogenicity assay of CoCbk1-AS strains on intact cucumber cotyledons after 7 d at 24°C. Conidial suspensions of indicated strains were prepared in distilled water or in distilled water containing 0.5 μM 1NA-PP1 and dropped onto detached cucumber cotyledons. (F) Development of infection structures of CoCbk1-AS strains on lower surface of detached cucumber cotyledons treated with 0.5 μM 1NA-PP1 at indicated times after start of incubation. Cells were observed at 3 d after inoculation. Control, incubated in distilled water for 3 d; Scale bar, 10 μm. Penetration hyphae were stained with lactophenol aniline blue. (G) Mean percentage (±SE) of normal appressoria and penetration hyphae of CoCbk1-AS strains on lower surface of detached cucumber cotyledons. Cells were treated with 0.5 μM 1NA-PP1 at the indicated times and observed at 3 d after inoculation. At least 300 appressoria on three cotyledons were observed in each of three independent experiments. Values are means of three replications. Control, incubated in distilled water for 3 d.

    Article Snippet: All C . orbiculare strains were maintained at 24°C in the dark on 3.9% (w/v) PDA (Difco) or SD medium (0.67% w/v yeast nitrogen base without amino acids, 2% w/v glucose, 2% w/v agar).

    Techniques: Infection, Sequencing, Binding Assay, Incubation, Staining

    Expression of the ech42-gox (A) and nag1-gox (B) fusions in T. atroviride P1 after 66 h of growth at 24°C in the dark on four different media. The activity is expressed in units per milliliter of extraction buffer per square centimeter of mycelial surface. Trichoderma was grown alone (open bars) or was coinoculated with the non-DON-producing F. graminearum strain GZT40 (solid bars). The media (1.5 and 0.1% malt extract agar, PDA, and SM medium) are described in Materials and Methods. Each value is the mean of three experiments with six replicate plates. Bars with different letters above them are significantly different according to Fisher's LSD test ( P ≤ 0.05).

    Journal: Applied and Environmental Microbiology

    Article Title: Mycotoxigenic Fusarium and Deoxynivalenol Production Repress Chitinase Gene Expression in the Biocontrol Agent Trichoderma atroviride P1

    doi: 10.1128/AEM.69.6.3077-3084.2003

    Figure Lengend Snippet: Expression of the ech42-gox (A) and nag1-gox (B) fusions in T. atroviride P1 after 66 h of growth at 24°C in the dark on four different media. The activity is expressed in units per milliliter of extraction buffer per square centimeter of mycelial surface. Trichoderma was grown alone (open bars) or was coinoculated with the non-DON-producing F. graminearum strain GZT40 (solid bars). The media (1.5 and 0.1% malt extract agar, PDA, and SM medium) are described in Materials and Methods. Each value is the mean of three experiments with six replicate plates. Bars with different letters above them are significantly different according to Fisher's LSD test ( P ≤ 0.05).

    Article Snippet: Four days before the start of experiments, Trichoderma strains were grown on PDA (4.8 g of potato dextrose broth [Difco, Detroit, Mich.], 12 g of agar [Oxoid, Basingstoke, Hampshire, United Kingdom]; pH 6.5) at 24°C in the dark.

    Techniques: Expressing, Activity Assay

    TSA enhances the antibiotic activity of T . atroviride mycelium-free culture filtrates (MFCF) against R . solani . T . atroviride was grown in PDB amended or not with 300 nM TSA, incubated for 7 days at 28 °C. The MFCFs were added to PDA 1× medium at a final concentration of 60%. R . solani radial growth was measured at 12, 24, 36, 48, and 60 h post-inoculation. R . solani growth on PDA (black bars), PDA plates amended with 300 nM TSA (arrow filled bars), PDA plates amended with 60% of T . atroviride MFCF (crosshatched bars), and PDA plates amended with 60% of T . atroviride MFCF grown in PDB plus TSA (black dotted bars) are shown. The bars show the mean ± SD of three independent biological replicates. Different letters are used to indicate means that differ significantly ( P

    Journal: PLoS ONE

    Article Title: Histone acetyltransferase TGF-1 regulates Trichoderma atroviride secondary metabolism and mycoparasitism

    doi: 10.1371/journal.pone.0193872

    Figure Lengend Snippet: TSA enhances the antibiotic activity of T . atroviride mycelium-free culture filtrates (MFCF) against R . solani . T . atroviride was grown in PDB amended or not with 300 nM TSA, incubated for 7 days at 28 °C. The MFCFs were added to PDA 1× medium at a final concentration of 60%. R . solani radial growth was measured at 12, 24, 36, 48, and 60 h post-inoculation. R . solani growth on PDA (black bars), PDA plates amended with 300 nM TSA (arrow filled bars), PDA plates amended with 60% of T . atroviride MFCF (crosshatched bars), and PDA plates amended with 60% of T . atroviride MFCF grown in PDB plus TSA (black dotted bars) are shown. The bars show the mean ± SD of three independent biological replicates. Different letters are used to indicate means that differ significantly ( P

    Article Snippet: Fungal strains were routinely grown in potato dextrose agar (PDA) or potato dextrose broth (PDB) (both from Difco™ , BD Becton, Dickinson and Company, New Jersey, USA), as indicated for each experiment.

    Techniques: Activity Assay, Incubation, Concentration Assay

    Antibiosis assay using MFCF obtained from T . atroviride wt or Δ tgf-1 strains grown in the presence or absence of TSA against R . solani . The Δ tgf-1 and wt strains were grown for 7 days in PDB medium amended or not with 300 nM TSA, at 28 °C. MFCFs obtained from each of these cultures were added to PDA 1× medium at a final concentration of 60%. R . solani was inoculated into the different media and its radial growth was determined at 12, 24, 36, 48, and 60 h. Radial growth of R . solani on PDA containing T . atroviride wt strain MFCF without TSA (black bars) or amended with TSA (crosshatched bars) was determined at the indicated times. Radial growth of R . solani on PDA containing T . atroviride Δ tgf-1 strain MFCF without TSA (black dotted bars) or with TSA (arrow filled bars) was determined at the indicated times. The bars show the mean ± SD of three independent biological replicates. Different letters are used to indicate means that differ significantly ( P

    Journal: PLoS ONE

    Article Title: Histone acetyltransferase TGF-1 regulates Trichoderma atroviride secondary metabolism and mycoparasitism

    doi: 10.1371/journal.pone.0193872

    Figure Lengend Snippet: Antibiosis assay using MFCF obtained from T . atroviride wt or Δ tgf-1 strains grown in the presence or absence of TSA against R . solani . The Δ tgf-1 and wt strains were grown for 7 days in PDB medium amended or not with 300 nM TSA, at 28 °C. MFCFs obtained from each of these cultures were added to PDA 1× medium at a final concentration of 60%. R . solani was inoculated into the different media and its radial growth was determined at 12, 24, 36, 48, and 60 h. Radial growth of R . solani on PDA containing T . atroviride wt strain MFCF without TSA (black bars) or amended with TSA (crosshatched bars) was determined at the indicated times. Radial growth of R . solani on PDA containing T . atroviride Δ tgf-1 strain MFCF without TSA (black dotted bars) or with TSA (arrow filled bars) was determined at the indicated times. The bars show the mean ± SD of three independent biological replicates. Different letters are used to indicate means that differ significantly ( P

    Article Snippet: Fungal strains were routinely grown in potato dextrose agar (PDA) or potato dextrose broth (PDB) (both from Difco™ , BD Becton, Dickinson and Company, New Jersey, USA), as indicated for each experiment.

    Techniques: Concentration Assay

    HadV1 transmission to single conidial subisolates of F. oxysporum isolate 7n and growth comparison between virus-free and -infected colonies. (A) Detection of HadV1 genomic RNA segments by RT-PCR using purified ssRNA as a template. RNA1 and other genomic segments, which are difficult to separate on an agarose gel, were targeted by RT-PCR. The ef1 α gene of F. oxysporum was employed as an internal control. HadV1(−) and HadV1(+) were confirmed to be virus negative and virus positive by direct colony one-step RT-PCR (see Fig. S6B in the supplemental material). The five subisolates representing each of the HadV1(−) and HadV1(+) groups were selected from conidia harvested from young (2- to 4-day-old) mycelia of the 7n strain. (B) Electrophoretic gel profile of dsRNA (viral replicative form) from HadV1(−) and HadV1(+) subisolates. (C) Colony morphology of 6-day-old cultures of HadV1(−) and HadV1(+) subisolates on PDA medium. (D) Areas of 6-day-old PDA cultures of HadV1(−) and HadV1(+) subisolates. The colony areas shown in Fig. 1C were measured using ImageJ ( http://imagej.nih.gov/ij/ ). The mean values ± standard deviations (SD) from five replicates are shown. Differences between the HadV1(−) and HadV1(+) subisolates were statistically analyzed by two-way analysis of variance (ANOVA) (Bartlett’s test) followed by a t test ( n = 5; P

    Journal: mBio

    Article Title: Hadaka Virus 1: a Capsidless Eleven-Segmented Positive-Sense Single-Stranded RNA Virus from a Phytopathogenic Fungus, Fusarium oxysporum

    doi: 10.1128/mBio.00450-20

    Figure Lengend Snippet: HadV1 transmission to single conidial subisolates of F. oxysporum isolate 7n and growth comparison between virus-free and -infected colonies. (A) Detection of HadV1 genomic RNA segments by RT-PCR using purified ssRNA as a template. RNA1 and other genomic segments, which are difficult to separate on an agarose gel, were targeted by RT-PCR. The ef1 α gene of F. oxysporum was employed as an internal control. HadV1(−) and HadV1(+) were confirmed to be virus negative and virus positive by direct colony one-step RT-PCR (see Fig. S6B in the supplemental material). The five subisolates representing each of the HadV1(−) and HadV1(+) groups were selected from conidia harvested from young (2- to 4-day-old) mycelia of the 7n strain. (B) Electrophoretic gel profile of dsRNA (viral replicative form) from HadV1(−) and HadV1(+) subisolates. (C) Colony morphology of 6-day-old cultures of HadV1(−) and HadV1(+) subisolates on PDA medium. (D) Areas of 6-day-old PDA cultures of HadV1(−) and HadV1(+) subisolates. The colony areas shown in Fig. 1C were measured using ImageJ ( http://imagej.nih.gov/ij/ ). The mean values ± standard deviations (SD) from five replicates are shown. Differences between the HadV1(−) and HadV1(+) subisolates were statistically analyzed by two-way analysis of variance (ANOVA) (Bartlett’s test) followed by a t test ( n = 5; P

    Article Snippet: The F. oxysporum strains described below were subcultured on Difco potato dextrose agar (PDA) medium (Becton, Dickinson and Co.).

    Techniques: Transmission Assay, Infection, Reverse Transcription Polymerase Chain Reaction, Purification, Agarose Gel Electrophoresis