post seeding (Tecan Systems)
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Post Seeding, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 17567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/post+seeding/Spark/pm39374781-188-2-9
Average 99 stars, based on 17567 article reviews
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Bioluminescence Resonance Energy Transfer:Article Title: Experimental variables determine the outcome of RAS-RAS interactions. Article Snippet: RAS clustering at the cell membrane is critical to activate signaling in cells, but whether this clustering is mediated exclusively by its c-terminal hypervariable region, receives contributions from the G-domain of RAS, and/or is influenced by secondary effectors has been intensely debated.. Reports that G-domain mutations do not modulate RAS-RAS interactions, have led some to question the validity of previous experiments that indicate the G-domain plays a role in RAS clustering/interactions.. Here we reconcile these findings by clarifying the impact of experimental variables, such as protein expression levels, cellular context, RAS zygosity, and secondary effector interactions on RAS clustering. Article Title: Experimental variables determine the outcome of RAS-RAS interactions Article Snippet: 24 h post-seeding, the medium was exchanged to no phenol red DMEM (Gibco) containing variable concentrations of FCS and drug or DMSO control. .. ∼40 h Concentration Assay:Article Title: Experimental variables determine the outcome of RAS-RAS interactions. Article Snippet: RAS clustering at the cell membrane is critical to activate signaling in cells, but whether this clustering is mediated exclusively by its c-terminal hypervariable region, receives contributions from the G-domain of RAS, and/or is influenced by secondary effectors has been intensely debated.. Reports that G-domain mutations do not modulate RAS-RAS interactions, have led some to question the validity of previous experiments that indicate the G-domain plays a role in RAS clustering/interactions.. Here we reconcile these findings by clarifying the impact of experimental variables, such as protein expression levels, cellular context, RAS zygosity, and secondary effector interactions on RAS clustering. Article Title: Experimental variables determine the outcome of RAS-RAS interactions Article Snippet: 24 h post-seeding, the medium was exchanged to no phenol red DMEM (Gibco) containing variable concentrations of FCS and drug or DMSO control. .. ∼40 h |
