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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Gl7 Positive Populations, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
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umap acoustic positioning software  (Lotek Wireless Inc)
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Electromyography (Emg) Research, supplied by MyoLearn, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Positive Colonies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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exosome positive markers  (Exosome Diagnostics)
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Exosome Positive Markers, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Findings Position Galectin 3, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rubisco positive control  (Agrisera)
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using <t>GL7,</t> follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.
Rubisco Positive Control, supplied by Agrisera, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using GL7, follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.

Journal: STAR Protocols

Article Title: Protocol for visualization and quantitative analysis of murine lung immunological architecture using iBALT-UNMAP following bacterial challenge

doi: 10.1016/j.xpro.2026.104472

Figure Lengend Snippet: TSA multiplex staining Schematic workflow representing the steps required for TSA multiplex staining. In this example we use first a staining using GL7, follwed by CD3 for T cells, and finally B220 for B cells. We add the colors used for each secondary to guide each cycle. Incubation times, washing, and stripping steps are included.

Article Snippet: Immune “Surfaces” tables for B220, CD3, and GL7 with object IDs and centroid coordinates. c. A lineage or class label per nucleus if you already assigned B cell, T cell, and GL7 positive populations in Imaris.

Techniques: Multiplex Assay, Staining, Incubation, Stripping Membranes