porcine intestinal heparin  (Millipore)


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    Structured Review

    Millipore porcine intestinal heparin
    Porcine Intestinal Heparin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/porcine intestinal heparin/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    porcine intestinal heparin - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Enzymatic synthesis of heparin related polysaccharides on sensor chips: Rapid screening of heparin–protein interactions
    Article Snippet: Heparosan was purified from E. coli K5 strain (from American Type Culture Collection) as described in a previous report [ ]. .. Porcine intestinal heparin (sodium salt) was from Sigma (St. Louis, MO).

    Article Title: Kinetic studies on the interactions of heparin and complement proteins using surface plasmon resonance
    Article Snippet: Porcine intestinal heparin (sodium salt) and 3, 3′-dithio-bis(propionic acid N -hydroxysuccinimide ester) (DTSP-NHS) were from Sigma (St. Louis, MO, USA). mPEG-SH and HCl·H2 N-PEG-COOH were from Nektar (Huntsville, AL, USA). .. Purified human complement proteins: C1 (MW 774 KD), C2 (MW 93 KD) and C3 (MW 190 KD) were from Calbiochem (La Jolla, CA) and are functionally pure; C5 (MW 190 KD, 1 mg/ml in PBS), C6 (MW 128 KD, 1 mg/ml in PBS), C7 (MW 121 KD, 1 mg/ml in PBS), C8 (MW 163 KD, 1 mg/ml in PBS), C9 (MW 79 KD, 1 mg/ml in PBS), factor I (MW 88 KD, 1.8 mg/ml in PBS), factor P (MW 220 KD, 1.0 mg/ml in PBS), factor B (MW 85.5 KD, 1.09 mg/ml in PBS pH 7.2), factor H (MW 150 KD, 1.05 mg/ml in PBS), and factor D (24 KD, 0.1–0.12 mg/ml in PBS pH 7.2) were all purchased from QUIDEL Corp (San Diego, CA, USA).

    Gel Permeation Chromatography:

    Article Title: Enzymatic synthesis of heparin related polysaccharides on sensor chips: Rapid screening of heparin–protein interactions
    Article Snippet: Porcine intestinal heparin (sodium salt) was from Sigma (St. Louis, MO). .. DEAE Sephadex ion exchange resin (50 g) and S200 HR Sephacryl GPC resin (250 ml) were from Sigma (St. Louis, MO).

    SDS Page:

    Article Title: Kinetic studies on the interactions of heparin and complement proteins using surface plasmon resonance
    Article Snippet: Porcine intestinal heparin (sodium salt) and 3, 3′-dithio-bis(propionic acid N -hydroxysuccinimide ester) (DTSP-NHS) were from Sigma (St. Louis, MO, USA). mPEG-SH and HCl·H2 N-PEG-COOH were from Nektar (Huntsville, AL, USA). .. Factors P, B, H, D and I were ≥85% and the remaining complement proteins from QUIDEL were ≥95% pure by SDS-PAGE.

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    Millipore porcine intestinal mucosa
    Porcine Intestinal Mucosa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
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    99/100 stars
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    85
    Millipore biotinylated porcine intestinal heparin
    Accumulation of chemokines on cell surfaces is mediated by oligomerization and GAG binding. <t>Biotinylated</t> WT and mutant chemokine (CCL5 and CCL5(E66S) dimer ( A , 500 nM), CXCL4 and CXCL4(K50E) dimer ( B , 500 nM) and CXCL8 and CXCL8(R26C) dimer ( C , 125 nM))
    Biotinylated Porcine Intestinal Heparin, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated porcine intestinal heparin/product/Millipore
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotinylated porcine intestinal heparin - by Bioz Stars, 2020-04
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    99
    Millipore heparin sepharose
    Accumulation of chemokines on cell surfaces is mediated by oligomerization and GAG binding. <t>Biotinylated</t> WT and mutant chemokine (CCL5 and CCL5(E66S) dimer ( A , 500 nM), CXCL4 and CXCL4(K50E) dimer ( B , 500 nM) and CXCL8 and CXCL8(R26C) dimer ( C , 125 nM))
    Heparin Sepharose, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparin sepharose/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    heparin sepharose - by Bioz Stars, 2020-04
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    Image Search Results


    Accumulation of chemokines on cell surfaces is mediated by oligomerization and GAG binding. Biotinylated WT and mutant chemokine (CCL5 and CCL5(E66S) dimer ( A , 500 nM), CXCL4 and CXCL4(K50E) dimer ( B , 500 nM) and CXCL8 and CXCL8(R26C) dimer ( C , 125 nM))

    Journal: Glycobiology

    Article Title: The dependence of chemokine–glycosaminoglycan interactions on chemokine oligomerization

    doi: 10.1093/glycob/cwv100

    Figure Lengend Snippet: Accumulation of chemokines on cell surfaces is mediated by oligomerization and GAG binding. Biotinylated WT and mutant chemokine (CCL5 and CCL5(E66S) dimer ( A , 500 nM), CXCL4 and CXCL4(K50E) dimer ( B , 500 nM) and CXCL8 and CXCL8(R26C) dimer ( C , 125 nM))

    Article Snippet: HS from bovine kidney (Sigma), CS-A (Sigma), 2-O-desulfated heparin and 6-O-desulfated heparin (Neoparin Inc.) were biotinylated as described previously ( ) and biotinylated porcine intestinal heparin (average molecular mass = 15 kDa) was purchased (Calbiochem).

    Techniques: Binding Assay, Mutagenesis