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pooled donor huvec (PromoCell)

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PromoCell pooled donor huvec
Pooled Donor Huvec, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pooled+donor+huvec/Human+Umbilical+Vein+Endothelial+Cells+(HUVEC)+single+donor/bio_rxiv__64898__2026__01__16__699995-60-1-4
Average 98 stars, based on 1576 article reviews

pooled donor huvec - by Bioz Stars, 2026-09

98/100 stars

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Cell Culture:

Article Title: Combinations of RAS pathway inhibitors with targeted agents are active in spheroids of patient-derived cells with oncogenic KRAS variants from multiple cancer types
Article Snippet: The established cell line HOP-62 was cultured in RPMI 1640 medium, HEPES (Invitrogen, cat. 22400105) with 10% defined fetal bovine serum (HyClone Laboratories Inc., cat. SH30070.03). .. The pooled donor HUVEC were cultured in endothelial cell growth medium 2 (PromoCell, Heidelberg, Germany, cat. C-22011) and the hMSC were cultured in mesenchymal stem cell growth medium 2 (PromoCell, cat. C-28009). ..

Article Title: Combination screen in multi-cell type tumor spheroids reveals interaction between aryl hydrocarbon receptor antagonists and E1 ubiquitin-activating enzyme inhibitor: Aryl-hydrocarbon receptor antagonist drug combinations.
Article Snippet: The established cell lines 786-O, A498, DMS-114, Hs 578T, NCI-H196, and OVCAR-5 were cultured in RPMI 1640 medium, HEPES (Invitrogen, cat. 22400105) with 10% defined fetal bovine serum (HyClone Laboratories Inc., cat. SH30070.03) and 2 mM L-glutamine (Invitrogen, cat. 25030081). .. The pooled donor HUVEC were cultured in endothelial cell growth medium 2 (PromoCell, Heidelberg, Germany, cat. C-22011), while the hMSC were cultured in mesenchymal stem cell growth medium 2 (PromoCell, cat. C-28009). ..

Article Title: Drug combinations with apoptosis pathway targeted agents alrizomadlin, pelcitoclax, and dasminapant in multi-cell type tumor spheroids
Article Snippet: NCI-H1876 was cultured in DMEM/F-12, HEPES (Thermo Fisher Scientific, cat. 11330032), 5% defined fetal bovine serum (HyClone Laboratories Inc., cat. SH30070.03), ITS premix universal culture supplement [insulin (5 μg/mL), transferrin (5 μg/mL), and selenious acid (5 ng/mL)] (Corning Inc., cat. 354350), 10 nM hydrocortisone (Sigma-Aldrich, cat. H6909), 10 nM β-estradiol (Sigma-Aldrich, cat. E2257), and 4.5 mM L-glutamine (Thermo Fisher Scientific, cat. 25030081). .. The pooled donor HUVEC were cultured in endothelial cell growth medium 2 (PromoCell, Heidelberg, Germany, cat. C-22011), while the hMSC were cultured in mesenchymal stem cell growth medium 2 (PromoCell, cat. C-28009). ..

Article Title: Combinatorial screen with apoptosis pathway targeted agents alrizomadlin, pelcitoclax, and dasminapant in multi-cell type tumor spheroids.
Article Snippet: NCI-H1876 was cultured in DMEM/F-12, HEPES (Thermo Fisher Scientific, cat. 11330032), 5% defined fetal bovine serum (HyClone Laboratories Inc., cat. SH30070.03), ITS premix universal culture supplement [insulin (5 μg/mL), transferrin (5 μg/mL), and selenious acid (5 ng/mL)] (Corning Inc., cat. 354350), 10 nM hydrocortisone (Sigma-Aldrich, cat. H6909), 10 nM β-estradiol (Sigma-Aldrich, cat. E2257), and 4.5 mM L-glutamine (Thermo Fisher Scientific, cat. 25030081). .. The pooled donor HUVEC were cultured in endothelial cell growth medium 2 (PromoCell, Heidelberg, Germany, cat. C-22011), while the hMSC were cultured in mesenchymal stem cell growth medium 2 (PromoCell, cat. C-28009). ..

Article Title: RAS Pathway Inhibitors Combined with Targeted Agents Are Active in Patient-Derived Spheroids with Oncogenic KRAS Variants from Multiple Cancer Types
Article Snippet: The established cell line HOP-62 was cultured in RPMI 1640 medium, HEPES (Invitrogen, cat. # 22400105), with 10% defined FBS (HyClone Laboratories Inc., cat. # SH30070.03). .. The pooled donor HUVEC were cultured in endothelial cell growth medium 2 (PromoCell, cat. # C-22011), and the hMSC were cultured in mesenchymal stem cell growth medium 2 (PromoCell, cat. # C-28009). ..

Labeling:

Article Title: Scalable Generation of Universal hiPSC-Derived Vascular Progenitor Cells for Safe and Sustained Revascularization in Chronic Limb-Threatening Ischemia
Article Snippet: Media was collected, centrifuged to remove any dead cells, and supernatants were analyzed using the Angiogenesis Array G1000 (Cat. No AAH-ANG-G1000-4) which was scanned and quantified at RayBioTech or with the following human Quantikine ELISA (R&D Systems) kits according to manufacturer’s instructions: HGF (DHG00B), CCL2/MCP-1 (CDP00), ANGPT2 (DANG20), ANGPT1 (DANG10), FGF2/bFGF (DFB50), CXCL12/SDF1 (DSA00), FST/follistatin (DFN00), ANG/angiogenin (DNA00), MMP2 (DMP2F0), EGF (DEG00) and ANGPTL4 (Invitrogen, EHANGPTL4). .. Primary, pooled donor HUVEC (Promocell, C-12203) were labeled with CellTrace-Far Red (ThermoFisher, C34564) at 1:100 in PBS for 20min at 37°C. ..

Image Search Results

YAP was upregulated in senescent endothelial cells (A) The proliferation of HUVECs population doubling level (PDL) at 10 and 30 were treated with vehicle or verteporfin (0.5 μM) for 12 h. Immunoblotting and quantifying the protein level of P16, P21, P53, VCAM1, and YAP. n = 4. (B) Proliferation of HUVECs PDL at 10 were transfected with siRNA-NC (negative control) or siRNA against YAP for 12 h, followed by treated with vehicle or H 2 O 2 (100 μM) for 12 h. Immunoblotting and quantifying the protein level of P16, P21, P53, VCAM1, and YAP. n = 4. (C) Proliferation of HUVECs PDL at 10 were infected with GFP adenovirus (Ad-GFP) or YAP adenovirus (Ad-YAP) for 24 h and then treated with vehicle or verteporfin (0.5 μM) for 12 h. Immunoblotting and quantifying the protein level of P16, P21, P53, VCAM1, and YAP. n = 4. (D and E) SA-β-Gal staining and quantitative analysis of senescent cells, scale bars: 40 μm, n = 6, p values correspond to one-way ANOVA with Tukey’s multiple comparisons test. All data are represented as mean ± SEM. ∗, p ≤ 0.05; ∗∗, p ≤ 0.01; ∗∗∗, p ≤ 0.001; ∗∗∗∗, p ≤ 0.0001.

Journal: iScience

Article Title: UFMylation maintains YAP stability to promote vascular endothelial cell senescence

doi: 10.1016/j.isci.2025.111854

Figure Lengend Snippet: YAP was upregulated in senescent endothelial cells (A) The proliferation of HUVECs population doubling level (PDL) at 10 and 30 were treated with vehicle or verteporfin (0.5 μM) for 12 h. Immunoblotting and quantifying the protein level of P16, P21, P53, VCAM1, and YAP. n = 4. (B) Proliferation of HUVECs PDL at 10 were transfected with siRNA-NC (negative control) or siRNA against YAP for 12 h, followed by treated with vehicle or H 2 O 2 (100 μM) for 12 h. Immunoblotting and quantifying the protein level of P16, P21, P53, VCAM1, and YAP. n = 4. (C) Proliferation of HUVECs PDL at 10 were infected with GFP adenovirus (Ad-GFP) or YAP adenovirus (Ad-YAP) for 24 h and then treated with vehicle or verteporfin (0.5 μM) for 12 h. Immunoblotting and quantifying the protein level of P16, P21, P53, VCAM1, and YAP. n = 4. (D and E) SA-β-Gal staining and quantitative analysis of senescent cells, scale bars: 40 μm, n = 6, p values correspond to one-way ANOVA with Tukey’s multiple comparisons test. All data are represented as mean ± SEM. ∗, p ≤ 0.05; ∗∗, p ≤ 0.01; ∗∗∗, p ≤ 0.001; ∗∗∗∗, p ≤ 0.0001.

Article Snippet: HUVEC (pooled donor) endothelial cells , Lonza , Cat# CC-2519.

Techniques: Western Blot, Transfection, Negative Control, Infection, Staining

YAP was identified as the substrate for UFMylation (A and B) Immunoblotting and quantification of the level of the indicated protein in HUVECs of the proliferation of PDL at 10 and 30; immunoblotting and quantification of levels of the indicated protein in aortic endothelial cells from 2 (young) or 24 (aged) months old mice. n = 3. (C) HEK293T cells were co-transfected with UFMylation components (UBA5, UFC1, UFL1, and DDRGK1) or wild-type (UFM1WT) or active UFM1 (UFM1ΔC2) or defective UFM1 (UFM1ΔC3) and FLAG-tagged YAP for detection exogenous YAP UFMylation. (D) Isolation of aortic endothelial cells from 8-week-old C57BL/6J mice for detection endogenous YAP UFMylation. (E) Immunofluorescence staining of YAP (green), UFM1 (red), and DAPI (blue) in HUVECs of the proliferation of PDL at 10 and 30, and serum stimulation as control, scale bars: 20 μm. (F) Isolated the nucleus and cytoplasmic proteins from young and senescent HUVECs, immunoblotting and quantification of levels YAP. n = 4. (G) UFMylation detection of YAP was identified using a PLA with UFM1 and YAP antibodies. n = 6, p values correspond to unpaired two-tailed Student’s t test. All data are represented as mean ± SEM. ∗, p ≤ 0.05; ∗∗, p ≤ 0.01; ∗∗∗, p ≤ 0.001; ∗∗∗∗, p ≤ 0.0001.

Journal: iScience

Article Title: UFMylation maintains YAP stability to promote vascular endothelial cell senescence

doi: 10.1016/j.isci.2025.111854

Figure Lengend Snippet: YAP was identified as the substrate for UFMylation (A and B) Immunoblotting and quantification of the level of the indicated protein in HUVECs of the proliferation of PDL at 10 and 30; immunoblotting and quantification of levels of the indicated protein in aortic endothelial cells from 2 (young) or 24 (aged) months old mice. n = 3. (C) HEK293T cells were co-transfected with UFMylation components (UBA5, UFC1, UFL1, and DDRGK1) or wild-type (UFM1WT) or active UFM1 (UFM1ΔC2) or defective UFM1 (UFM1ΔC3) and FLAG-tagged YAP for detection exogenous YAP UFMylation. (D) Isolation of aortic endothelial cells from 8-week-old C57BL/6J mice for detection endogenous YAP UFMylation. (E) Immunofluorescence staining of YAP (green), UFM1 (red), and DAPI (blue) in HUVECs of the proliferation of PDL at 10 and 30, and serum stimulation as control, scale bars: 20 μm. (F) Isolated the nucleus and cytoplasmic proteins from young and senescent HUVECs, immunoblotting and quantification of levels YAP. n = 4. (G) UFMylation detection of YAP was identified using a PLA with UFM1 and YAP antibodies. n = 6, p values correspond to unpaired two-tailed Student’s t test. All data are represented as mean ± SEM. ∗, p ≤ 0.05; ∗∗, p ≤ 0.01; ∗∗∗, p ≤ 0.001; ∗∗∗∗, p ≤ 0.0001.

Article Snippet: HUVEC (pooled donor) endothelial cells , Lonza , Cat# CC-2519.

Techniques: Western Blot, Transfection, Isolation, Immunofluorescence, Staining, Control, Two Tailed Test

UFMylation of YAP slows down its protein degeneration speed and inhibiting UFMylation could prevent endothelial cell senescence (A and B) Proliferation of HUVECs PDL at 10 were transfected with siRNA-NC (negative control) or siRNA against UFL1/UFM1 for 36 h. Immunoblotting and quantifying the protein level of YAP, n = 4, p values correspond to one-way ANOVA with Tukey’s multiple comparisons test. (C and D) The proliferation of HUVECs PDL at 10 and 30 were treated with cycloheximide (CHX) (10 μM) for the indicated time. Immunoblotting and quantifying the protein level of YAP, n = 3, p values correspond to two-way ANOVA with Sidak’s multiple comparisons test. (E and F) Proliferation of HUVECs PDL at 30 were treated with vehicle or compound 8.5 (20 μM) for 12 h, followed by treated with CHX (10 μM) for the indicated time. Immunoblotting and quantifying the protein level of YAP, n = 3, p values correspond to two-way ANOVA with Sidak’s multiple comparisons test. (G and J) Proliferation of HUVECs PDL at 10 were treated with vehicle or H 2 O 2 (100 μM) for 12 h, followed by treated with vehicle, verteporfin (0.5 μM), compound 8.5 (20 μM) or verteporfin (0.5 μM), and compound 8.5 (20 μM) for 12 h. n = 3. (G and H) Immunoblotting and quantifying the indicated protein levels. (I and J) SA-β-Gal staining and quantitative analysis of senescent cells, scale bars: 40 μm, n = 6, p values correspond to one-way ANOVA with Tukey’s multiple comparisons test. All data are represented as mean ± SEM. ∗, p ≤ 0.05; ∗∗, p ≤ 0.01; ∗∗∗, p ≤ 0.001.

Journal: iScience

Article Title: UFMylation maintains YAP stability to promote vascular endothelial cell senescence

doi: 10.1016/j.isci.2025.111854

Figure Lengend Snippet: UFMylation of YAP slows down its protein degeneration speed and inhibiting UFMylation could prevent endothelial cell senescence (A and B) Proliferation of HUVECs PDL at 10 were transfected with siRNA-NC (negative control) or siRNA against UFL1/UFM1 for 36 h. Immunoblotting and quantifying the protein level of YAP, n = 4, p values correspond to one-way ANOVA with Tukey’s multiple comparisons test. (C and D) The proliferation of HUVECs PDL at 10 and 30 were treated with cycloheximide (CHX) (10 μM) for the indicated time. Immunoblotting and quantifying the protein level of YAP, n = 3, p values correspond to two-way ANOVA with Sidak’s multiple comparisons test. (E and F) Proliferation of HUVECs PDL at 30 were treated with vehicle or compound 8.5 (20 μM) for 12 h, followed by treated with CHX (10 μM) for the indicated time. Immunoblotting and quantifying the protein level of YAP, n = 3, p values correspond to two-way ANOVA with Sidak’s multiple comparisons test. (G and J) Proliferation of HUVECs PDL at 10 were treated with vehicle or H 2 O 2 (100 μM) for 12 h, followed by treated with vehicle, verteporfin (0.5 μM), compound 8.5 (20 μM) or verteporfin (0.5 μM), and compound 8.5 (20 μM) for 12 h. n = 3. (G and H) Immunoblotting and quantifying the indicated protein levels. (I and J) SA-β-Gal staining and quantitative analysis of senescent cells, scale bars: 40 μm, n = 6, p values correspond to one-way ANOVA with Tukey’s multiple comparisons test. All data are represented as mean ± SEM. ∗, p ≤ 0.05; ∗∗, p ≤ 0.01; ∗∗∗, p ≤ 0.001.

Article Snippet: HUVEC (pooled donor) endothelial cells , Lonza , Cat# CC-2519.

Techniques: Transfection, Negative Control, Western Blot, Staining

Journal: iScience

Article Title: UFMylation maintains YAP stability to promote vascular endothelial cell senescence

doi: 10.1016/j.isci.2025.111854

Figure Lengend Snippet:

Article Snippet: HUVEC (pooled donor) endothelial cells , Lonza , Cat# CC-2519.

Techniques: Control, Virus, Recombinant, Protease Inhibitor, Transfection, Bicinchoninic Acid Protein Assay, Western Blot, Staining, Negative Control, Software, Membrane

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