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Bio-Rad polyvinylidene fluoride membranes pvdf
Polyvinylidene Fluoride Membranes Pvdf, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyvinylidene fluoride membranes pvdf/product/Bio-Rad
Average 89 stars, based on 11 article reviews
Price from $9.99 to $1999.99
polyvinylidene fluoride membranes pvdf - by Bioz Stars, 2021-01
89/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Lico A Enhances Nrf2-Mediated Defense Mechanisms against t-BHP-Induced Oxidative Stress and Cell Death via Akt and ERK Activation in RAW 264.7 Cells
Article Snippet: .. An equal amount of protein (40 μ g) for each sample was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 10% gel and then electrophoretically transferred onto a polyvinylidene difluoride membranes (PVDF), which was purchased from Bio-Rad (Hercules, CA). .. The membrane was blocked with blocking solution (5% (w/v) nonfat dry milk) for 2 h and followed by an overnight incubation at 4°C with specific primary antibody, including Keap1, Nrf2, HO-1, p-Akt/Akt, p-JNK/JNK, p-ERK/ERK, p-p38/p38, Lamin B, and β -actin.

Electrotransfer:

Article Title: Pyrroloquinoline Quinone Resists Denervation-Induced Skeletal Muscle Atrophy by Activating PGC-1α and Integrating Mitochondrial Electron Transport Chain Complexes
Article Snippet: .. Samples (20 μg) of total cell lysates were electrophoretically size fractionated by a 10% polyacrylamide sodium dodecyl sulfate–polyacrylamide gel and transferred onto a polyvinylidene difluoride membrane (PVDF) using the BioRad Mini Protean electrotransfer system. .. The blots were subsequently incubated with 5% skim milk in phosphate-buffered saline with Tween-20 for 1 h to block non-specific binding and probed overnight at 4°C with specific antibodies against PGC-1α, mitochondrial transcription factor A (TFAM), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and β-actin (Biochiefdom, Taipei, Taiwan), respectively.

Blocking Assay:

Article Title: ?-Thymosins and Hemocyte Homeostasis in a Crustacean
Article Snippet: .. Western blot analysis Protein samples were dissolved in Laemmli sample buffer (62.5 mM Tris-HCl, 2% SDS, 10% (v/v) glycerol, 0.1 M DTT, 0.01% bromophenol blue, pH 6.8) and separated using a 12.5% SDS-PAGE and then electro-transferred onto a polyvinylidene fluoride membrane (PVDF) (Bio-Rad, America) for 2 h. The blot was blocked subsequently in Tris buffered saline (TBST) (0.5% Tween 20 in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5) containing 5% skim milk for 1 h. The membrane was incubated with anti-ATP synthase β subunit (Santa Cruz Biotechnology ) (dilution 1∶3000) or anti-Ast1 (dilution 1∶1000) as previously described by Lin et al or anti-Tβ4 (Tβ-4 (FL-44): sc-67114, Santa Cruz Biotechnology)(dilution 1:7000) in blocking buffer for 1 h at RT. .. After extensive washing, the membrane was incubated with a secondary antibody conjugated with horseradish peroxidase (Sigma) (dilution 1∶7,500), and detection was performed using the ECL western blotting reagent kit (Amersham Biosciences) according to the manufacturer’s instructions.

Electrophoresis:

Article Title: CARI III Inhibits Tumor Growth in a Melanoma-Bearing Mouse Model through Induction of G0/G1 Cell Cycle Arrest
Article Snippet: .. Total protein (25 μg) was separated by electrophoresis on a 12% SDS-PAGE polyacrylamide gel and then electrophoretically transferred onto polyvinylidene fluoride membranes (PVDF) (Bio-Rad Laboratories, Berkeley, CA, USA). ..

Immunoprecipitation:

Article Title: RhoH Regulates Subcellular Localization of ZAP-70 and Lck in T Cell Receptor Signaling
Article Snippet: .. The immunoprecipitated mixture or 20 µg total lysates were separated on a SDS polyacrylamide gel, and transferred to a polyvinylidene difluoride membrane (PVDF) (BioRad). .. For Lck kinase assay, anti-Lck immunoprecipitate products were washed three times with lysis buffer and once with kinase buffer (50 mM Tris pH 7.5, 5 mM MgCl2 , 5 mM MnCl2 , 1 mM Na3 VO4 and 1 mM DTT) and resuspended with 30 µL kinase buffer.

Incubation:

Article Title: ?-Thymosins and Hemocyte Homeostasis in a Crustacean
Article Snippet: .. Western blot analysis Protein samples were dissolved in Laemmli sample buffer (62.5 mM Tris-HCl, 2% SDS, 10% (v/v) glycerol, 0.1 M DTT, 0.01% bromophenol blue, pH 6.8) and separated using a 12.5% SDS-PAGE and then electro-transferred onto a polyvinylidene fluoride membrane (PVDF) (Bio-Rad, America) for 2 h. The blot was blocked subsequently in Tris buffered saline (TBST) (0.5% Tween 20 in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5) containing 5% skim milk for 1 h. The membrane was incubated with anti-ATP synthase β subunit (Santa Cruz Biotechnology ) (dilution 1∶3000) or anti-Ast1 (dilution 1∶1000) as previously described by Lin et al or anti-Tβ4 (Tβ-4 (FL-44): sc-67114, Santa Cruz Biotechnology)(dilution 1:7000) in blocking buffer for 1 h at RT. .. After extensive washing, the membrane was incubated with a secondary antibody conjugated with horseradish peroxidase (Sigma) (dilution 1∶7,500), and detection was performed using the ECL western blotting reagent kit (Amersham Biosciences) according to the manufacturer’s instructions.

Western Blot:

Article Title: ?-Thymosins and Hemocyte Homeostasis in a Crustacean
Article Snippet: .. Western blot analysis Protein samples were dissolved in Laemmli sample buffer (62.5 mM Tris-HCl, 2% SDS, 10% (v/v) glycerol, 0.1 M DTT, 0.01% bromophenol blue, pH 6.8) and separated using a 12.5% SDS-PAGE and then electro-transferred onto a polyvinylidene fluoride membrane (PVDF) (Bio-Rad, America) for 2 h. The blot was blocked subsequently in Tris buffered saline (TBST) (0.5% Tween 20 in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5) containing 5% skim milk for 1 h. The membrane was incubated with anti-ATP synthase β subunit (Santa Cruz Biotechnology ) (dilution 1∶3000) or anti-Ast1 (dilution 1∶1000) as previously described by Lin et al or anti-Tβ4 (Tβ-4 (FL-44): sc-67114, Santa Cruz Biotechnology)(dilution 1:7000) in blocking buffer for 1 h at RT. .. After extensive washing, the membrane was incubated with a secondary antibody conjugated with horseradish peroxidase (Sigma) (dilution 1∶7,500), and detection was performed using the ECL western blotting reagent kit (Amersham Biosciences) according to the manufacturer’s instructions.

Staining:

Article Title: Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training
Article Snippet: .. Proteins were subsequently transferred (200 mA for 2 h) to polyvinylidene difluoride membranes (PVDF) (Bio-Rad Laboratories, Hercules, CA, USA), Ponceau stained, and imaged to ensure equal protein loading between lanes. ..

SDS Page:

Article Title: Lico A Enhances Nrf2-Mediated Defense Mechanisms against t-BHP-Induced Oxidative Stress and Cell Death via Akt and ERK Activation in RAW 264.7 Cells
Article Snippet: .. An equal amount of protein (40 μ g) for each sample was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 10% gel and then electrophoretically transferred onto a polyvinylidene difluoride membranes (PVDF), which was purchased from Bio-Rad (Hercules, CA). .. The membrane was blocked with blocking solution (5% (w/v) nonfat dry milk) for 2 h and followed by an overnight incubation at 4°C with specific primary antibody, including Keap1, Nrf2, HO-1, p-Akt/Akt, p-JNK/JNK, p-ERK/ERK, p-p38/p38, Lamin B, and β -actin.

Article Title: ?-Thymosins and Hemocyte Homeostasis in a Crustacean
Article Snippet: .. Western blot analysis Protein samples were dissolved in Laemmli sample buffer (62.5 mM Tris-HCl, 2% SDS, 10% (v/v) glycerol, 0.1 M DTT, 0.01% bromophenol blue, pH 6.8) and separated using a 12.5% SDS-PAGE and then electro-transferred onto a polyvinylidene fluoride membrane (PVDF) (Bio-Rad, America) for 2 h. The blot was blocked subsequently in Tris buffered saline (TBST) (0.5% Tween 20 in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5) containing 5% skim milk for 1 h. The membrane was incubated with anti-ATP synthase β subunit (Santa Cruz Biotechnology ) (dilution 1∶3000) or anti-Ast1 (dilution 1∶1000) as previously described by Lin et al or anti-Tβ4 (Tβ-4 (FL-44): sc-67114, Santa Cruz Biotechnology)(dilution 1:7000) in blocking buffer for 1 h at RT. .. After extensive washing, the membrane was incubated with a secondary antibody conjugated with horseradish peroxidase (Sigma) (dilution 1∶7,500), and detection was performed using the ECL western blotting reagent kit (Amersham Biosciences) according to the manufacturer’s instructions.

Article Title: CARI III Inhibits Tumor Growth in a Melanoma-Bearing Mouse Model through Induction of G0/G1 Cell Cycle Arrest
Article Snippet: .. Total protein (25 μg) was separated by electrophoresis on a 12% SDS-PAGE polyacrylamide gel and then electrophoretically transferred onto polyvinylidene fluoride membranes (PVDF) (Bio-Rad Laboratories, Berkeley, CA, USA). ..

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    Bio-Rad pvdf membrane
    Relative Accumulation of MatR and Various Mitochondrial Proteins during Arabidopsis Seed Germination. (A) ). Detection was performed by chemiluminescence with the Image Quant LAS4000 mini analyzer (GE Healthcare). The intensities of protein signals in (A) and (B) ). (B) <t>-PAGE</t> analysis of the respiratory chain complexes during seed germination in Arabidopsis. Crude membrane fractions obtained from dry seeds, imbibed seeds, and mature Arabidopsis seedlings were solubilized with DDM ( n ). For immunodetections, the proteins were transferred from the native gels onto a <t>PVDF</t> membrane (Bio-Rad) in cathode buffer for 15 h at 40 mA, using the Bio-Rad mini transblot cell. The membranes were distained with ethanol before probing with specific antibodies, as indicated below each blot. Arrows indicate the native respiratory complexes, CI (∼1000 kD), CIII (dimer, ∼500 kD), CIV (∼220 kD), and CV (∼600 kD), in Arabidopsis mitochondria. Please note, in (B) , the original COX2 blot has been modified, i.e., the lane corresponding of the mature leaves (M) was cut from the right side of the blot and pasted to the other side (marked with dotted line). No other changes have been made to the original figure.
    Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvdf membrane/product/Bio-Rad
    Average 99 stars, based on 3488 article reviews
    Price from $9.99 to $1999.99
    pvdf membrane - by Bioz Stars, 2021-01
    99/100 stars
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    Pkg of 1 roll 0 2 µm 26 cm x 3 3 m bulk membrane for high binding 150 160 µg cm2 immunoblotting
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    Relative Accumulation of MatR and Various Mitochondrial Proteins during Arabidopsis Seed Germination. (A) ). Detection was performed by chemiluminescence with the Image Quant LAS4000 mini analyzer (GE Healthcare). The intensities of protein signals in (A) and (B) ). (B) -PAGE analysis of the respiratory chain complexes during seed germination in Arabidopsis. Crude membrane fractions obtained from dry seeds, imbibed seeds, and mature Arabidopsis seedlings were solubilized with DDM ( n ). For immunodetections, the proteins were transferred from the native gels onto a PVDF membrane (Bio-Rad) in cathode buffer for 15 h at 40 mA, using the Bio-Rad mini transblot cell. The membranes were distained with ethanol before probing with specific antibodies, as indicated below each blot. Arrows indicate the native respiratory complexes, CI (∼1000 kD), CIII (dimer, ∼500 kD), CIV (∼220 kD), and CV (∼600 kD), in Arabidopsis mitochondria. Please note, in (B) , the original COX2 blot has been modified, i.e., the lane corresponding of the mature leaves (M) was cut from the right side of the blot and pasted to the other side (marked with dotted line). No other changes have been made to the original figure.

    Journal: The Plant Cell

    Article Title: The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria

    doi: 10.1105/tpc.16.00398

    Figure Lengend Snippet: Relative Accumulation of MatR and Various Mitochondrial Proteins during Arabidopsis Seed Germination. (A) ). Detection was performed by chemiluminescence with the Image Quant LAS4000 mini analyzer (GE Healthcare). The intensities of protein signals in (A) and (B) ). (B) -PAGE analysis of the respiratory chain complexes during seed germination in Arabidopsis. Crude membrane fractions obtained from dry seeds, imbibed seeds, and mature Arabidopsis seedlings were solubilized with DDM ( n ). For immunodetections, the proteins were transferred from the native gels onto a PVDF membrane (Bio-Rad) in cathode buffer for 15 h at 40 mA, using the Bio-Rad mini transblot cell. The membranes were distained with ethanol before probing with specific antibodies, as indicated below each blot. Arrows indicate the native respiratory complexes, CI (∼1000 kD), CIII (dimer, ∼500 kD), CIV (∼220 kD), and CV (∼600 kD), in Arabidopsis mitochondria. Please note, in (B) , the original COX2 blot has been modified, i.e., the lane corresponding of the mature leaves (M) was cut from the right side of the blot and pasted to the other side (marked with dotted line). No other changes have been made to the original figure.

    Article Snippet: For nondenaturing-PAGE-protein gel blotting, the gel was transferred to a PVDF membrane (Bio-Rad) in Cathode buffer (50 mM Tricine and 15 mM Bis-Tris-HCl, pH 7.0) for 16 h at 4°C (constant current of 40 mA).

    Techniques: Polyacrylamide Gel Electrophoresis, Modification

    RBMY antibody confirmation by using Western-blot analysis of recombinant RBMY. Two concentrations (100 and 10 ng) of each recombinant protein were loaded on SDS-PAGE and after separation were transferred to PVDF membrane. Blots were exposed to X-ray films

    Journal: Iranian Biomedical Journal

    Article Title: Expression Analysis of RNA-Binding Motif Gene on Y Chromosome (RBMY) Protein Isoforms in Testis Tissue and a Testicular Germ Cell Cancer-Derived Cell Line (NT2)

    doi: 10.6091/ibj.1148.2013

    Figure Lengend Snippet: RBMY antibody confirmation by using Western-blot analysis of recombinant RBMY. Two concentrations (100 and 10 ng) of each recombinant protein were loaded on SDS-PAGE and after separation were transferred to PVDF membrane. Blots were exposed to X-ray films

    Article Snippet: Total protein extract (40 µg) from each sample was separated on 12% SDS-polyacrylamide gels for 120 min at 100 V and transferred to PVDF membrane (Bio-Rad, USA) by a wet transfer system (Bio-Rad, USA) at 20 V overnight.

    Techniques: Western Blot, Recombinant, SDS Page

    Compounds 33 and 38 bind Aβ and reduce AβO formation, but have no effect on Aβ production. (A) Representative western blot. Cells were treated were lysed, and proteins were separated by SDS-PAGE. After transfer to a PVDF membrane, blots were probed with the 6E10 antibody. (B) Quantification of total Aβ oligomers from western blotting. Error bars represent SEM. (n=6; ** p

    Journal: Bioorganic & medicinal chemistry

    Article Title: Bivalent ligands incorporating curcumin and diosgenin as multifunctional compounds against Alzheimer’s disease

    doi: 10.1016/j.bmc.2015.10.032

    Figure Lengend Snippet: Compounds 33 and 38 bind Aβ and reduce AβO formation, but have no effect on Aβ production. (A) Representative western blot. Cells were treated were lysed, and proteins were separated by SDS-PAGE. After transfer to a PVDF membrane, blots were probed with the 6E10 antibody. (B) Quantification of total Aβ oligomers from western blotting. Error bars represent SEM. (n=6; ** p

    Article Snippet: Equal amounts of protein (20.0 μg) were separated by SDS-PAGE on a Tris-Tricine gel (Bio-Rad) and transferred onto a PVDF membrane (Bio-Rad).

    Techniques: Western Blot, SDS Page

    Relative accumulation of organellar proteins in wild-type and mterf22 plants. (A) Immunoblot analyses of wild-type plants and mterf22-1 mutant line. For the quantification of the relative abundances of organellar proteins in mterf22 plants, different amounts of total mitochondrial proteins extracted from wild-type plants were loaded and separated by SDS-PAGE. The blots were probed with polyclonal antibodies raised to different organellar proteins, as indicated in each panel. Detection was carried out by chemiluminescence assays after incubation with HRP-conjugated secondary antibody. (B) BN-PAGE of crude mitochondria preparations was performed according to the method described in [ 57 ]. Crude mitochondria preparations, obtained from 3-week-old Arabidopsis seedlings, were solubilized with DDM [1.5% (w/v)] and the organellar complexes were resolved by BN-PAGE. For immunodetection, proteins were transferred from the native gels onto a PVDF membrane and were probed with specific antibodies ( S2 Table ), as indicated below each blot. Arrows indicate to the native complexes I (~1,000 kDa), III (dimer, ~500 kDa), IV (~220 kDa) and V (~600 kDa). The asterisk in the CA2 panel indicates to the presence of a 700 ~ 800 kDa band, which may corresponds to a complex I assembly intermediate. Hybridization signals were analyzed by chemiluminescence assays after incubation with HRP-conjugated secondary antibody. The intensities of protein signals in panels ‘A’ and ‘B’ using ImageJ software [ 90 ].

    Journal: PLoS ONE

    Article Title: Control of organelle gene expression by the mitochondrial transcription termination factor mTERF22 in Arabidopsis thaliana plants

    doi: 10.1371/journal.pone.0201631

    Figure Lengend Snippet: Relative accumulation of organellar proteins in wild-type and mterf22 plants. (A) Immunoblot analyses of wild-type plants and mterf22-1 mutant line. For the quantification of the relative abundances of organellar proteins in mterf22 plants, different amounts of total mitochondrial proteins extracted from wild-type plants were loaded and separated by SDS-PAGE. The blots were probed with polyclonal antibodies raised to different organellar proteins, as indicated in each panel. Detection was carried out by chemiluminescence assays after incubation with HRP-conjugated secondary antibody. (B) BN-PAGE of crude mitochondria preparations was performed according to the method described in [ 57 ]. Crude mitochondria preparations, obtained from 3-week-old Arabidopsis seedlings, were solubilized with DDM [1.5% (w/v)] and the organellar complexes were resolved by BN-PAGE. For immunodetection, proteins were transferred from the native gels onto a PVDF membrane and were probed with specific antibodies ( S2 Table ), as indicated below each blot. Arrows indicate to the native complexes I (~1,000 kDa), III (dimer, ~500 kDa), IV (~220 kDa) and V (~600 kDa). The asterisk in the CA2 panel indicates to the presence of a 700 ~ 800 kDa band, which may corresponds to a complex I assembly intermediate. Hybridization signals were analyzed by chemiluminescence assays after incubation with HRP-conjugated secondary antibody. The intensities of protein signals in panels ‘A’ and ‘B’ using ImageJ software [ 90 ].

    Article Snippet: For immunoblotting of non-denaturing PAGE, the proteins were transferred from the gel onto a PVDF membrane (Bio-Rad) in Cathode buffer (50 mM Tricine, 15 mM Bis-Tris-HCl, pH 7.0) for 16 h at 4°C at constant current of 40 mA.

    Techniques: Mutagenesis, SDS Page, Incubation, Polyacrylamide Gel Electrophoresis, Immunodetection, Hybridization, Software