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TaKaRa polymerase chain reaction
Polymerase Chain Reaction, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction/product/TaKaRa
Average 93 stars, based on 42 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction - by Bioz Stars, 2020-08
93/100 stars

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Polymerase Chain Reaction:

Article Title: PD-1 in human NK cells: evidence of cytoplasmic mRNA and protein expression
Article Snippet: .. PCR for PD-1 isoforms To detect PD-1 mRNA transcripts isoforms, cDNAs were amplified using the EmeralAMP® GT PCR Master Mix (Takara Bio Inc., Kusatsu, Japan). .. Amplification was carried out with the ProFlex PCR system (Applied Biosystems, Foster City, CA, USA) using the following cycling profile: an initial hold at 95°C for 30 sec, 40 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min; final extension step of 72°C for 10 min.

Amplification:

Article Title: PD-1 in human NK cells: evidence of cytoplasmic mRNA and protein expression
Article Snippet: .. PCR for PD-1 isoforms To detect PD-1 mRNA transcripts isoforms, cDNAs were amplified using the EmeralAMP® GT PCR Master Mix (Takara Bio Inc., Kusatsu, Japan). .. Amplification was carried out with the ProFlex PCR system (Applied Biosystems, Foster City, CA, USA) using the following cycling profile: an initial hold at 95°C for 30 sec, 40 cycles of 94°C for 1 min, 64°C for 1 min and 72°C for 1 min; final extension step of 72°C for 10 min.

other:

Article Title: Identification of Kinases and Phosphatases That Regulate ATG4B Activity by siRNA and Small Molecule Screening in Cells
Article Snippet: All PCR were performed at 30 cycles using Pyrobest DNA polymerase (Takara, R005A).

Article Title: Phylogeny, biogeography and taxonomic re-assessment of Multifurca (Russulaceae, Russulales) using three-locus data
Article Snippet: For this sample, PCR product was cloned using the Takara® pMDTM 18T cloning kit (Dalian, China) following the manufacturer’s instruction.

Article Title: TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb
Article Snippet: After two rounds of nested PCR utilizing Phanta Max, the PCR products were proceeded to deoxyadenosine (A)-tailing at the 3'-end of the PCR products utilizing Ex Taq DNA polymerase (Takara) without thermal cycling as follows: 95°C, 5 min; 72°C, 30 min; 4°C hold.

Article Title: Hnf4α Is Involved in LC-PUFA Biosynthesis by Up-Regulating Gene Transcription of Elongase in Marine Teleost Siganus canaliculatus
Article Snippet: After two rounds of PCR, the PCR product of the upstream sequence was recovered and isolated by gel extraction, then inserted into the pMD18-T Vector (TaKaRa Bio, Tokyo, Japan), and sequenced (Sangon Biotech Co., Ltd., Shanghai, China).

Article Title: Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen
Article Snippet: The PCR product was then ligated into the BglII/BamHI sites of pEGFP-C1 vector (Clontech Laboratories, Mountain View, CA, USA).

Article Title: TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb
Article Snippet: After two rounds of nested PCR utilizing Phanta Max, the PCR products were proceeded to deoxyadenosine (A)-tailing at the 3'-end of the PCR products utilizing Ex Taq DNA polymerase (Takara) without thermal cycling as follows: 95℃, 5 min; 72℃, 30 min; 4℃ hold.

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  • 93
    TaKaRa bcl 2
    Western blot analysis of the protein expressions of Notch, CTNNB1, Bax, <t>Bcl-2,</t> BIM, Hes1, Runx2, and osteocalcin in each transfected group ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each group. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P
    Bcl 2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bcl 2/product/TaKaRa
    Average 93 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    bcl 2 - by Bioz Stars, 2020-08
    93/100 stars
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    92
    TaKaRa mirna microarray assay total rna
    miR-143 and miR-145 expression in CA samples. ( a ) <t>Microarray</t> data of <t>miRNAs</t> in CA samples compared with self-controls ( n = 5 pairs). ( b ) Expression of candidate miRNAs were detected for CA samples in a larger scale, using qRT-PCR ( n = 60 in CA samples and n = 20 in control group). ( c ) Expression of miR-143 or miR-145 in CA samples using qRT-PCR. Data were expressed as log2 values ( n = 60 in CA samples and n = 20 in control group). ( d–f ) Inverse relationships between expression of miR-143 or miR-145 and course of disease or recurrence rate were revealed.
    Mirna Microarray Assay Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirna microarray assay total rna/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mirna microarray assay total rna - by Bioz Stars, 2020-08
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    92
    TaKaRa mirnas
    Antiviral mechanism of mja-miR-35 in shrimp. (A) Predicted target genes of mja-miR-35. As predicted, the 3′ UTRs of wsv140, wsv279, wsv309 , and wsv361 genes were targeted by mja-miR-35. The seed sequence of mja-miR-35 was underlined. (B) Constructs of the wild-type and mutated 3′ UTRs of the viral gene. The sequences complementary to the seed sequence of mja-miR-35 or the mutated seed sequences were underlined. (C) Interaction between mja-miR-35 and the viral wsv140, wsv279, wsv309 , and wsv361 genes in insect cells. The insect High Five cells were co-transfected with mja-miR-35 and different plasmids (EGFP-3′ UTR or EGFP-3′ UTR-mutant). As a control, mja-miR-35-scrambled was included in the transfection. At 48 h after transfection, fluorescent images (upper panel) and fluorescence intensities (lower panel) were obtained. Lane headings indicated the plasmids used. The <t>miRNAs</t> for transfections were shown on the left. Scale bar, 10 μm. (D) Overexpression of mja-miR-35 in shrimp. Shrimp were co-injected with WSSV and mja-miR-35 or mja-miR-35-scrambled. PBS and WSSV alone were used as controls. At 48 h after injection, shrimp hemocytes were subjected to Northern blotting with mja-miR-35-specific probe. U6 was used as a loading control. (E) Effects of mja-miR-35 overexpression on viral gene expression in vivo . WSSV- infected shrimp were injected with mja-miR-35 or scrambled-miRNA. At 24 h and 48 h after injection, the shrimp hemocytes were subjected to quantitative real-time PCR to examine the expression levels of wsv140, wsv279, wsv309 , and wsv361 genes. (F) Silencing of virus gene expression in shrimp. Sequence-specific siRNA was injected into WSSV-infected shrimp to knock down the expression of virus genes. At different time points after injection, the shrimp hemocytes were analyzed using quantitative real-time PCR. WSSV alone and scrambled-siRNA were used as controls. (G) Influence of virus gene silencing on WSSV replication. Gene-specific siRNA or scrambled-siRNA was injected into WSSV-infected shrimp. At different time points after infection, the hemocytes of shrimp were subjected to quantitative real-time PCR analysis to detect WSSV copies. WSSV alone was used as a positive control. (H) Effects of virus gene silencing on the mortality of WSSV-infected shrimp. The shrimp were injected with different <t>siRNAs.</t> The shrimp mortality was monitored daily. WSSV alone was used as a control. In all panels, the statistically significant difference between treatments was indicated with asterisks (* p
    Mirnas, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirnas/product/TaKaRa
    Average 92 stars, based on 289 article reviews
    Price from $9.99 to $1999.99
    mirnas - by Bioz Stars, 2020-08
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    91
    TaKaRa arabidopsis tissues
    Expression pattern of EMB1990/YLMG1-1 gene in <t>Arabidopsis</t> tissues and organs. (A) Expression levels of EMB1990/YLMG1-1 genes in different tissues by qPCR assay. R, root; S, stem; L, leave; Sl, seedling; In, inflorescence; F, flower; 1Si, 1 DAP silique; 2Si, 2 DAP silique; 3Si, 3 DAP silique; 4Si, 4 DAP silique; 5Si, 5 DAP silique; 6Si, 6 DAP silique; 7Si, 7 DAP silique. (B–F) GUS activity in pYLMG1-1::GUS transgenic plants. (B) Flower; (C) inflorescence; (D) 7 DAG seedling; (E) 14 DAG seedling; (F) rosette leave. Scale bars = 2mm. (G–J) Fluorescence analysis of embryos at different stage from pYLMG1-1::YLMG1-1-Venus transgenic plants. (G) Globular stage; (H) Heart stage; (G) torpedo stage; (G) bent cotyledon stage. Scale bars = 20 μm.
    Arabidopsis Tissues, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Western blot analysis of the protein expressions of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each transfected group ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each group. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: Western blot analysis of the protein expressions of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each transfected group ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each group. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Western Blot, Transfection, Expressing

    RT-qPCR detection of miR-340 expression and relative mRNA expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each transfected group * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: RT-qPCR detection of miR-340 expression and relative mRNA expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each transfected group * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Quantitative RT-PCR, Expressing, Transfection

    Immunohistochemistry and positive expression rate of CTNNB1 and Bcl-2 protein in OS and normal bone tissue ( A ) Immunohistochemical images (×200) of CTNNB1 in normal bone and OS tissues. ( B ) Positive expression rate of CTNNB1 in normal bone and OS tissues. ( C ) Immunohistochemical images (×200) of Bcl-2 in normal bone and OS tissues. ( D ) Positive expression rate of Bcl-2 in normal bone and OS tissues; * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: Immunohistochemistry and positive expression rate of CTNNB1 and Bcl-2 protein in OS and normal bone tissue ( A ) Immunohistochemical images (×200) of CTNNB1 in normal bone and OS tissues. ( B ) Positive expression rate of CTNNB1 in normal bone and OS tissues. ( C ) Immunohistochemical images (×200) of Bcl-2 in normal bone and OS tissues. ( D ) Positive expression rate of Bcl-2 in normal bone and OS tissues; * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Immunohistochemistry, Expressing

    RT-qPCR detection of miR-340 expression and relative mRNA expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in OS and normal bone tissues * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: RT-qPCR detection of miR-340 expression and relative mRNA expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in OS and normal bone tissues * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Quantitative RT-PCR, Expressing

    Western blot analysis of the relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in OS and normal bone tissues ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: Western blot analysis of the relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in OS and normal bone tissues ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Western Blot, Expressing

    miR-143 and miR-145 expression in CA samples. ( a ) Microarray data of miRNAs in CA samples compared with self-controls ( n = 5 pairs). ( b ) Expression of candidate miRNAs were detected for CA samples in a larger scale, using qRT-PCR ( n = 60 in CA samples and n = 20 in control group). ( c ) Expression of miR-143 or miR-145 in CA samples using qRT-PCR. Data were expressed as log2 values ( n = 60 in CA samples and n = 20 in control group). ( d–f ) Inverse relationships between expression of miR-143 or miR-145 and course of disease or recurrence rate were revealed.

    Journal: Royal Society Open Science

    Article Title: Loss of miR-143 and miR-145 in condyloma acuminatum promotes cellular proliferation and inhibits apoptosis by targeting NRAS

    doi: 10.1098/rsos.172376

    Figure Lengend Snippet: miR-143 and miR-145 expression in CA samples. ( a ) Microarray data of miRNAs in CA samples compared with self-controls ( n = 5 pairs). ( b ) Expression of candidate miRNAs were detected for CA samples in a larger scale, using qRT-PCR ( n = 60 in CA samples and n = 20 in control group). ( c ) Expression of miR-143 or miR-145 in CA samples using qRT-PCR. Data were expressed as log2 values ( n = 60 in CA samples and n = 20 in control group). ( d–f ) Inverse relationships between expression of miR-143 or miR-145 and course of disease or recurrence rate were revealed.

    Article Snippet: 2.2. miRNA microarray assay Total RNA was extracted from HPV6b-positive CA tissues and HPV-negative foreskin tissues using RNAiso™ PLUS (TaKaRa, China) following the manufacturer's protocol.

    Techniques: Expressing, Microarray, Quantitative RT-PCR

    Antiviral mechanism of mja-miR-35 in shrimp. (A) Predicted target genes of mja-miR-35. As predicted, the 3′ UTRs of wsv140, wsv279, wsv309 , and wsv361 genes were targeted by mja-miR-35. The seed sequence of mja-miR-35 was underlined. (B) Constructs of the wild-type and mutated 3′ UTRs of the viral gene. The sequences complementary to the seed sequence of mja-miR-35 or the mutated seed sequences were underlined. (C) Interaction between mja-miR-35 and the viral wsv140, wsv279, wsv309 , and wsv361 genes in insect cells. The insect High Five cells were co-transfected with mja-miR-35 and different plasmids (EGFP-3′ UTR or EGFP-3′ UTR-mutant). As a control, mja-miR-35-scrambled was included in the transfection. At 48 h after transfection, fluorescent images (upper panel) and fluorescence intensities (lower panel) were obtained. Lane headings indicated the plasmids used. The miRNAs for transfections were shown on the left. Scale bar, 10 μm. (D) Overexpression of mja-miR-35 in shrimp. Shrimp were co-injected with WSSV and mja-miR-35 or mja-miR-35-scrambled. PBS and WSSV alone were used as controls. At 48 h after injection, shrimp hemocytes were subjected to Northern blotting with mja-miR-35-specific probe. U6 was used as a loading control. (E) Effects of mja-miR-35 overexpression on viral gene expression in vivo . WSSV- infected shrimp were injected with mja-miR-35 or scrambled-miRNA. At 24 h and 48 h after injection, the shrimp hemocytes were subjected to quantitative real-time PCR to examine the expression levels of wsv140, wsv279, wsv309 , and wsv361 genes. (F) Silencing of virus gene expression in shrimp. Sequence-specific siRNA was injected into WSSV-infected shrimp to knock down the expression of virus genes. At different time points after injection, the shrimp hemocytes were analyzed using quantitative real-time PCR. WSSV alone and scrambled-siRNA were used as controls. (G) Influence of virus gene silencing on WSSV replication. Gene-specific siRNA or scrambled-siRNA was injected into WSSV-infected shrimp. At different time points after infection, the hemocytes of shrimp were subjected to quantitative real-time PCR analysis to detect WSSV copies. WSSV alone was used as a positive control. (H) Effects of virus gene silencing on the mortality of WSSV-infected shrimp. The shrimp were injected with different siRNAs. The shrimp mortality was monitored daily. WSSV alone was used as a control. In all panels, the statistically significant difference between treatments was indicated with asterisks (* p

    Journal: Frontiers in Immunology

    Article Title: Shrimp Antiviral mja-miR-35 Targets CHI3L1 in Human M2 Macrophages and Suppresses Breast Cancer Metastasis

    doi: 10.3389/fimmu.2018.02071

    Figure Lengend Snippet: Antiviral mechanism of mja-miR-35 in shrimp. (A) Predicted target genes of mja-miR-35. As predicted, the 3′ UTRs of wsv140, wsv279, wsv309 , and wsv361 genes were targeted by mja-miR-35. The seed sequence of mja-miR-35 was underlined. (B) Constructs of the wild-type and mutated 3′ UTRs of the viral gene. The sequences complementary to the seed sequence of mja-miR-35 or the mutated seed sequences were underlined. (C) Interaction between mja-miR-35 and the viral wsv140, wsv279, wsv309 , and wsv361 genes in insect cells. The insect High Five cells were co-transfected with mja-miR-35 and different plasmids (EGFP-3′ UTR or EGFP-3′ UTR-mutant). As a control, mja-miR-35-scrambled was included in the transfection. At 48 h after transfection, fluorescent images (upper panel) and fluorescence intensities (lower panel) were obtained. Lane headings indicated the plasmids used. The miRNAs for transfections were shown on the left. Scale bar, 10 μm. (D) Overexpression of mja-miR-35 in shrimp. Shrimp were co-injected with WSSV and mja-miR-35 or mja-miR-35-scrambled. PBS and WSSV alone were used as controls. At 48 h after injection, shrimp hemocytes were subjected to Northern blotting with mja-miR-35-specific probe. U6 was used as a loading control. (E) Effects of mja-miR-35 overexpression on viral gene expression in vivo . WSSV- infected shrimp were injected with mja-miR-35 or scrambled-miRNA. At 24 h and 48 h after injection, the shrimp hemocytes were subjected to quantitative real-time PCR to examine the expression levels of wsv140, wsv279, wsv309 , and wsv361 genes. (F) Silencing of virus gene expression in shrimp. Sequence-specific siRNA was injected into WSSV-infected shrimp to knock down the expression of virus genes. At different time points after injection, the shrimp hemocytes were analyzed using quantitative real-time PCR. WSSV alone and scrambled-siRNA were used as controls. (G) Influence of virus gene silencing on WSSV replication. Gene-specific siRNA or scrambled-siRNA was injected into WSSV-infected shrimp. At different time points after infection, the hemocytes of shrimp were subjected to quantitative real-time PCR analysis to detect WSSV copies. WSSV alone was used as a positive control. (H) Effects of virus gene silencing on the mortality of WSSV-infected shrimp. The shrimp were injected with different siRNAs. The shrimp mortality was monitored daily. WSSV alone was used as a control. In all panels, the statistically significant difference between treatments was indicated with asterisks (* p

    Article Snippet: The miRNAs and siRNAs were synthesized using in vitro transcription T7 kit (Takara) according to the manufacturer′s instructions.

    Techniques: Sequencing, Construct, Transfection, Mutagenesis, Fluorescence, Over Expression, Injection, Northern Blot, Expressing, In Vivo, Infection, Real-time Polymerase Chain Reaction, Positive Control

    Expression pattern of EMB1990/YLMG1-1 gene in Arabidopsis tissues and organs. (A) Expression levels of EMB1990/YLMG1-1 genes in different tissues by qPCR assay. R, root; S, stem; L, leave; Sl, seedling; In, inflorescence; F, flower; 1Si, 1 DAP silique; 2Si, 2 DAP silique; 3Si, 3 DAP silique; 4Si, 4 DAP silique; 5Si, 5 DAP silique; 6Si, 6 DAP silique; 7Si, 7 DAP silique. (B–F) GUS activity in pYLMG1-1::GUS transgenic plants. (B) Flower; (C) inflorescence; (D) 7 DAG seedling; (E) 14 DAG seedling; (F) rosette leave. Scale bars = 2mm. (G–J) Fluorescence analysis of embryos at different stage from pYLMG1-1::YLMG1-1-Venus transgenic plants. (G) Globular stage; (H) Heart stage; (G) torpedo stage; (G) bent cotyledon stage. Scale bars = 20 μm.

    Journal: Frontiers in Plant Science

    Article Title: Arabidopsis EMB1990 Encoding a Plastid-Targeted YlmG Protein Is Required for Chloroplast Biogenesis and Embryo Development

    doi: 10.3389/fpls.2018.00181

    Figure Lengend Snippet: Expression pattern of EMB1990/YLMG1-1 gene in Arabidopsis tissues and organs. (A) Expression levels of EMB1990/YLMG1-1 genes in different tissues by qPCR assay. R, root; S, stem; L, leave; Sl, seedling; In, inflorescence; F, flower; 1Si, 1 DAP silique; 2Si, 2 DAP silique; 3Si, 3 DAP silique; 4Si, 4 DAP silique; 5Si, 5 DAP silique; 6Si, 6 DAP silique; 7Si, 7 DAP silique. (B–F) GUS activity in pYLMG1-1::GUS transgenic plants. (B) Flower; (C) inflorescence; (D) 7 DAG seedling; (E) 14 DAG seedling; (F) rosette leave. Scale bars = 2mm. (G–J) Fluorescence analysis of embryos at different stage from pYLMG1-1::YLMG1-1-Venus transgenic plants. (G) Globular stage; (H) Heart stage; (G) torpedo stage; (G) bent cotyledon stage. Scale bars = 20 μm.

    Article Snippet: RNA Extraction and RT-PCR Total RNA from different kinds of Arabidopsis tissues was isolated by RNAiso Plus, except the siliques and ovules were extracted using MiniBEST Plant RNA Extraction Kit (TaKaRa, Japan).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Activity Assay, Transgenic Assay, Fluorescence

    Characterization and complementation of Arabidopsis emb1990 mutants. (A) Schematic diagrams of EMB1990/YLMG1-1 gene. The positions of T-DNA insertions in emb1990-1 and emb1990-2 mutants are shown in the schematic diagrams. Arrowheads indicate the positions of primers used for genotyping. (B–F) Seed development in siliques of wild-type, mutants, and complemented plants. White arrows highlight the aborted white ovules, and the siliques were placed as morphological apical to basal from left to right. Bars = 1 mm.

    Journal: Frontiers in Plant Science

    Article Title: Arabidopsis EMB1990 Encoding a Plastid-Targeted YlmG Protein Is Required for Chloroplast Biogenesis and Embryo Development

    doi: 10.3389/fpls.2018.00181

    Figure Lengend Snippet: Characterization and complementation of Arabidopsis emb1990 mutants. (A) Schematic diagrams of EMB1990/YLMG1-1 gene. The positions of T-DNA insertions in emb1990-1 and emb1990-2 mutants are shown in the schematic diagrams. Arrowheads indicate the positions of primers used for genotyping. (B–F) Seed development in siliques of wild-type, mutants, and complemented plants. White arrows highlight the aborted white ovules, and the siliques were placed as morphological apical to basal from left to right. Bars = 1 mm.

    Article Snippet: RNA Extraction and RT-PCR Total RNA from different kinds of Arabidopsis tissues was isolated by RNAiso Plus, except the siliques and ovules were extracted using MiniBEST Plant RNA Extraction Kit (TaKaRa, Japan).

    Techniques: