Journal: Nature Communications
Article Title: Microglia and amyloid precursor protein coordinate control of transient Candida cerebritis with memory deficits
Figure Lengend Snippet: C. albicans cerebritis induces upregulation of amyloid precursor protein (APP). Mice were challenged with C. albicans as in Fig. 2 and RNA and protein extracted from whole brain. a qRT-PCR was performed to quantify mRNA for APP over 14 days. b Western blot analysis of APP over the same time period. c Densitometric analysis of the western blot data. d Immunofluorescence images of brain from C. albicans -infected wild-type mice 4 days post i.v. challenge with C. albicans showing staining for DAPI, IBA1, APP, NeuN, and merged images. Images are centered on fungal granulomas similar to those shown in Fig. 2 . e Repeat staining of fungal granulomas for DAPI, IBA1, APP, and calcofluor white (CW) comparing wild-type to app − / − mice. ( n = 4, mean ± S.E.M, * p
Article Snippet: Relative expression of mRNA for APP was detected by two-step, real-time quantitative reverse transcription-PCR (RT-PCR) with the ABI Perkin Elmer Prism 5700 Sequence Detection System (Applied Biosystems, Foster City, CA) using Taqman probe (Mm00476361, Mm01344172, Invitrogen, Carlsbad, CA).
Techniques: Mouse Assay, Quantitative RT-PCR, Western Blot, Immunofluorescence, Infection, Staining