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Roche polymerase chain reaction pcr
Polymerase Chain Reaction Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Isolation of an Equine Foamy Virus and Sero-Epidemiology of the Viral Infection in Horses in Japan
Article Snippet: Polymerase Chain Reaction (PCR) for Detection of the Equine Foamy Virus Genome To detect EFV DNA in cells showing a CPE, PCR assays targeting LTR were carried out using the Expand High Fidelity PCR system (Roche Diagnostic GmbH, Mannheim, Germany) and primers listed in , which were designed on the basis of the complete EFV sequence (GenBank AF201902) by using DNASIS Pro (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

Article Title: Outbreak of hepatitis C virus infection associated with narcotics diversion by an hepatitis C virus–infected surgical technician
Article Snippet: At the CDC, serum samples were tested for HCV RNA by polymerase chain reaction (PCR) using the AMPLICOR HCV Test, version 2.0 (Roche Molecular Systems, Branchburg, NJ), with a lower limit of detection of ~50 copies/mL.

Article Title: Cyclosporine A stimulated hair growth from mouse vibrissae follicles in an organ culture model
Article Snippet: Polymerase chain reaction (PCR) The specific target cDNA was amplified using the PCR Core Kit (Roche, Germany) and the following primers were used.

Article Title: A complex multilevel attack on Pseudomonas aeruginosa algT/U expression and AlgT/U activity results in the loss of alginate production
Article Snippet: Genomic DNA was prepared from PAO1, PDO300 and the sap strains and the polymerase chain reaction (PCR) was used to amplify the mucA , algT/U or algO loci using the Expand High Fidelity PCR kit (Roche Applied Science, Indianapolis, IN).

Article Title: Genetic comparison of Ug99 with selected South African races of Puccinia graminis f.sp. tritici
Article Snippet: Each 15 µL polymerase chain reaction (PCR) contained 10 ng total genomic DNA, 10 pmol of each primer and a 1 × concentration of KapaTaq ReadyMix (KapaBiosystems, Cape Town, South Africa).

Article Title: Synthesis and Structure-Activity Relationships of N-(2-Oxo-3-oxetanyl)amides as N-Acylethanolamine-hydrolyzing Acid Amidase Inhibitors
Article Snippet: We amplified the full-length coding sequence of rat NAAA by polymerase chain reaction (PCR) using High Fidelity PCR Master (Roche, Indianapolis, IN) and rat brain cDNAs as templates.

Article Title: MAP-1 and IAP-1, Two Novel AAA Proteases with Catalytic Sites on Opposite Membrane Surfaces in Mitochondrial Inner Membrane ofNeurospora crassa
Article Snippet: This DNA fragment was amplified by polymerase chain reaction (PCR) with the primer pair 5′-TTT GGA TCC CGT TCC GAC GGC GGC TTC AGG-3′ and 5′-TTT AAG CTT AGG ACT TGC GCT CCA GAC CGC-3′, and, after labeling with the DIG-DNA-Labeling kit (Roche, Mannheim, Germany), used as a probe for screening the N. crassa cosmid library pMOcosX ( ) by colony hybridization.

Article Title: Selective cytotoxicity and antifungal properties of copper(II) and cobalt(II) complexes with imidazole-4-acetate anion or 1-allylimidazole
Article Snippet: Analysis of metal complexes interaction with DNA The effect of metal complexes on DNA was measured by polymerase chain reaction (PCR) with high resolution melting (HRM) using LightCycler 480 II (Hoffman-LaRoche, Switzerland) .

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    Roche telomerase activity assays cancer cells
    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding <t>telomerase</t> activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.
    Telomerase Activity Assays Cancer Cells, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche plasma hbv dna
    Comparison of <t>HBV</t> replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV <t>DNA</t> from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p
    Plasma Hbv Dna, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche p malariae strains greece
    Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. <t>malariae</t> (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control
    P Malariae Strains Greece, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gfp  (Roche)
    92
    Roche gfp
    Colocalization and effects of direct NPFFR2 signalling on <t>NPY</t> neurons. a Representative image of <t>GFP</t> expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p
    Gfp, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Journal: Oncotarget

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    doi: 10.18632/oncotarget.25993

    Figure Lengend Snippet: STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Article Snippet: Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA.

    Techniques: Binding Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Journal: Oncotarget

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    doi: 10.18632/oncotarget.25993

    Figure Lengend Snippet: Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Article Snippet: Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA.

    Techniques: Activity Assay

    Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Journal: Viruses

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    doi: 10.3390/v11010078

    Figure Lengend Snippet: Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Article Snippet: The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

    Techniques: Mutagenesis, Transfection, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Journal: Viruses

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    doi: 10.3390/v11010078

    Figure Lengend Snippet: Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Article Snippet: The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

    Techniques: Mutagenesis, MANN-WHITNEY

    Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Titration, Infection, Incubation, Multiplex Assay, Concentration Assay

    Antibody competition titration assays using homologous MSP1 19 proteins. Dilutions (1:400) of P. falciparum Lot 6 defined human serum or of sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) were incubated with the indicated concentrations of the homologous MSP1 19 competitor protein for 1 h at room temperature. Multiplex bead assays were performed as described in “ Methods ”, and the multiplex responses in MFI-bg units are plotted versus the competitor concentration

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Antibody competition titration assays using homologous MSP1 19 proteins. Dilutions (1:400) of P. falciparum Lot 6 defined human serum or of sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) were incubated with the indicated concentrations of the homologous MSP1 19 competitor protein for 1 h at room temperature. Multiplex bead assays were performed as described in “ Methods ”, and the multiplex responses in MFI-bg units are plotted versus the competitor concentration

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Titration, Infection, Incubation, Multiplex Assay, Concentration Assay

    Alignment of predicted Plasmodium spp. MSP1 19 protein sequences using COBALT [ 61 ]. Residues in the P. malariae sequence that differ from the Cameroon sequence of Birkenmeyer et al. [ 38 ] are shaded. Predicted protein sequences resulting from the oligonucleotides used in PCR amplification are underlined. The positions of residues conserved among all the presented MSP1 19 protein sequences are indicated in the consensus with divergent residues indicated by a dot. GenBank accession numbers are MH577181, P. ovale Nigeria I strain; MH577182, P. malariae China I strain; MH577183, P. malariae Greece I strain; MH577184, P. malariae Uganda I strain; and MH577185, P. malariae Guyana strain

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Alignment of predicted Plasmodium spp. MSP1 19 protein sequences using COBALT [ 61 ]. Residues in the P. malariae sequence that differ from the Cameroon sequence of Birkenmeyer et al. [ 38 ] are shaded. Predicted protein sequences resulting from the oligonucleotides used in PCR amplification are underlined. The positions of residues conserved among all the presented MSP1 19 protein sequences are indicated in the consensus with divergent residues indicated by a dot. GenBank accession numbers are MH577181, P. ovale Nigeria I strain; MH577182, P. malariae China I strain; MH577183, P. malariae Greece I strain; MH577184, P. malariae Uganda I strain; and MH577185, P. malariae Guyana strain

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification

    Colocalization and effects of direct NPFFR2 signalling on NPY neurons. a Representative image of GFP expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p

    Journal: Nature Communications

    Article Title: Diet-induced adaptive thermogenesis requires neuropeptide FF receptor-2 signalling

    doi: 10.1038/s41467-018-06462-0

    Figure Lengend Snippet: Colocalization and effects of direct NPFFR2 signalling on NPY neurons. a Representative image of GFP expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p

    Article Snippet: RT-qPCR using primers for Npy , GFP and Npffr2 was carried out in samples prior (input) and after the immunoprecipitation in at least triplicates from 1:5 dilution cDNA from each sample using the LightCycler® (LightCycler® 480 Real-Time PCR system, Roche Applied Science, Germany), SYBR Green I (Molecular Probes) and Platinum Taq DNA Polymerase (Invitrogen).

    Techniques: Expressing, Immunoprecipitation, Isolation, Mouse Assay