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Axygen polymerase chain reaction pcr tubes
Polymerase Chain Reaction Pcr Tubes, supplied by Axygen, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr tubes/product/Axygen
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr tubes - by Bioz Stars, 2020-03
92/100 stars

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Related Articles

Nucleic Acid Electrophoresis:

Article Title: Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis
Article Snippet: : 1708195EDU); Chef-DR II Gel Electrophoresis System (BIO-RAD; Hercules, CA, United States; Cat.no. .. : 170-3612); Polymerase chain reaction (PCR) tubes (Axygen; Hercules, CA, United States; Cat. no.: 14-222-262); Rotaphor (Biometra; Gottingen, Germany; Cat.no.

Flow Cytometry:

Article Title: The volatile anesthetic isoflurane perturbs conformational activation of integrin LFA-1 by binding to the allosteric regulatory cavity
Article Snippet: Flow cytometry to detect the binding of soluble ICAM-1-fragment crystallizable α (Fcα)/anti-immunoglobulin A-fluorescein isothiocyanate (IgA-FITC) to LFA-1 on K562 transfectants or PBMCs was performed as described previously with minor modifications. .. Cells (5×105 ) in 300 μl HBS were aliquoted to polymerase chain reaction (PCR) tubes (Axygen, Union City, CA, USA) and then centrifuged.

Article Title: Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis
Article Snippet: : 13-400-519); BD Accuri C6 Plus Flow Cytometer (BD Biosciences; Hercules, CA, United States; Cat.no. .. : 170-3612); Polymerase chain reaction (PCR) tubes (Axygen; Hercules, CA, United States; Cat. no.: 14-222-262); Rotaphor (Biometra; Gottingen, Germany; Cat.no.

Cytometry:

Article Title: The volatile anesthetic isoflurane perturbs conformational activation of integrin LFA-1 by binding to the allosteric regulatory cavity
Article Snippet: Flow cytometry to detect the binding of soluble ICAM-1-fragment crystallizable α (Fcα)/anti-immunoglobulin A-fluorescein isothiocyanate (IgA-FITC) to LFA-1 on K562 transfectants or PBMCs was performed as described previously with minor modifications. .. Cells (5×105 ) in 300 μl HBS were aliquoted to polymerase chain reaction (PCR) tubes (Axygen, Union City, CA, USA) and then centrifuged.

Article Title: Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis
Article Snippet: : 13-400-519); BD Accuri C6 Plus Flow Cytometer (BD Biosciences; Hercules, CA, United States; Cat.no. .. : 170-3612); Polymerase chain reaction (PCR) tubes (Axygen; Hercules, CA, United States; Cat. no.: 14-222-262); Rotaphor (Biometra; Gottingen, Germany; Cat.no.

Concentration Assay:

Article Title: The volatile anesthetic isoflurane perturbs conformational activation of integrin LFA-1 by binding to the allosteric regulatory cavity
Article Snippet: Cells (5×105 ) in 300 μl HBS were aliquoted to polymerase chain reaction (PCR) tubes (Axygen, Union City, CA, USA) and then centrifuged. .. Cell pellets were given a 150 μl aliquot of HBS, 2 mM MnCl2 containing isoflurane at 2× final concentration, and another 150 μl aliquot of HBS containing 10 μg/ml ICAM-1-Fcα fusion protein or control human IgA, 25 μg/ml FITC conjugated goat anti-human IgA antibody (Pierce, Rockford, IL, USA).

Incubation:

Article Title: The volatile anesthetic isoflurane perturbs conformational activation of integrin LFA-1 by binding to the allosteric regulatory cavity
Article Snippet: Cells (5×105 ) in 300 μl HBS were aliquoted to polymerase chain reaction (PCR) tubes (Axygen, Union City, CA, USA) and then centrifuged. .. Tubes were immediately capped, incubated for 30 min at room temperature, and unbound ICAM-1-Fcα/anti-IgA-FITC was washed off with HBS.

Polymerase Chain Reaction:

Article Title: The volatile anesthetic isoflurane perturbs conformational activation of integrin LFA-1 by binding to the allosteric regulatory cavity
Article Snippet: .. Cells (5×105 ) in 300 μl HBS were aliquoted to polymerase chain reaction (PCR) tubes (Axygen, Union City, CA, USA) and then centrifuged. .. Cell pellets were given a 150 μl aliquot of HBS, 2 mM MnCl2 containing isoflurane at 2× final concentration, and another 150 μl aliquot of HBS containing 10 μg/ml ICAM-1-Fcα fusion protein or control human IgA, 25 μg/ml FITC conjugated goat anti-human IgA antibody (Pierce, Rockford, IL, USA).

Article Title: Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis
Article Snippet: .. : 170-3612); Polymerase chain reaction (PCR) tubes (Axygen; Hercules, CA, United States; Cat. no.: 14-222-262); Rotaphor (Biometra; Gottingen, Germany; Cat.no. .. : 846-021-101); Thermocycler (Super Cycler SC300G, Kyratec, Mansfield, Australia; Cat.no.

Chloramphenicol Acetyltransferase Assay:

Article Title: Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis
Article Snippet: .. : 170-3612); Polymerase chain reaction (PCR) tubes (Axygen; Hercules, CA, United States; Cat. no.: 14-222-262); Rotaphor (Biometra; Gottingen, Germany; Cat.no. .. : 846-021-101); Thermocycler (Super Cycler SC300G, Kyratec, Mansfield, Australia; Cat.no.

Binding Assay:

Article Title: The volatile anesthetic isoflurane perturbs conformational activation of integrin LFA-1 by binding to the allosteric regulatory cavity
Article Snippet: Paragraph title: Binding of soluble intercellular adhesion molecule 1 (ICAM-1) to LFA-1 on the cell surface ... Cells (5×105 ) in 300 μl HBS were aliquoted to polymerase chain reaction (PCR) tubes (Axygen, Union City, CA, USA) and then centrifuged.

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  • 99
    Axygen pcr tube
    T7E1 analyses of three potential off-target sites (zinc finger protein GLIS3 , ChrUScaf1915 , and dynamin-3-like ) in blastocysts no. 1 (lane 1 ), 2 (lane 2 ), and 10 (lane 3 ) together with normal <t>PEFs</t> (lane C ). Two bands (~140 and ~80 bp) are expected to be cleaved from the 220 bp <t>PCR</t> products of GLIS3 . Two bands of ~200 and 120 bp are expected to be cleaved from the 318 bp PCR products of ChrUScaf1915 . Two bands of ~200 and ~130 bp bands are expected to be cleaved from the 328 bp PCR products of dynamic-3-like . However, there is no appreciable off-target cleavage in the samples tested. M , 100 bp ladder markers.
    Pcr Tube, supplied by Axygen, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr tube/product/Axygen
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pcr tube - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    82
    Axygen sterile polypropylene pcr tubes
    Application of HBP-rVAP <t>polyclonal</t> antiserum in immunodiagnostics. a Specific decoration of bacilliform particles of CYMV in immunosorbent electron microscopy (ISEM) using the HBP-rVAP antiserum (1:100 dilution). b Immunocapture <t>PCR</t> (IC-PCR) using RT/RNaseH primers. CYMV-infected citrus samples trapped with HBp-rVAP antiserum. Lane M–O′ generuler ladder mix (Thermo Scientific, USA). Lanes: 1–3 at dilutions of 1:250, 4–6 at dilutions 1: 500, and 7–9 at dilutions 1:1000, respectively. Lanes 1, 4 and 7—amplification of CYMV specific genome of amplicon size ~ 589 bp. Lanes 2, 5, and 8—showed faint amplification of genome size ~ 589 bp. Lanes 3, 6 and 9—showed no amplification in healthy control
    Sterile Polypropylene Pcr Tubes, supplied by Axygen, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile polypropylene pcr tubes/product/Axygen
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sterile polypropylene pcr tubes - by Bioz Stars, 2020-03
    82/100 stars
      Buy from Supplier

    88
    Axygen polypropylene pcr tubes
    Application of HBP-rVAP <t>polyclonal</t> antiserum in immunodiagnostics. a Specific decoration of bacilliform particles of CYMV in immunosorbent electron microscopy (ISEM) using the HBP-rVAP antiserum (1:100 dilution). b Immunocapture <t>PCR</t> (IC-PCR) using RT/RNaseH primers. CYMV-infected citrus samples trapped with HBp-rVAP antiserum. Lane M–O′ generuler ladder mix (Thermo Scientific, USA). Lanes: 1–3 at dilutions of 1:250, 4–6 at dilutions 1: 500, and 7–9 at dilutions 1:1000, respectively. Lanes 1, 4 and 7—amplification of CYMV specific genome of amplicon size ~ 589 bp. Lanes 2, 5, and 8—showed faint amplification of genome size ~ 589 bp. Lanes 3, 6 and 9—showed no amplification in healthy control
    Polypropylene Pcr Tubes, supplied by Axygen, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene pcr tubes/product/Axygen
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    polypropylene pcr tubes - by Bioz Stars, 2020-03
    88/100 stars
      Buy from Supplier

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    T7E1 analyses of three potential off-target sites (zinc finger protein GLIS3 , ChrUScaf1915 , and dynamin-3-like ) in blastocysts no. 1 (lane 1 ), 2 (lane 2 ), and 10 (lane 3 ) together with normal PEFs (lane C ). Two bands (~140 and ~80 bp) are expected to be cleaved from the 220 bp PCR products of GLIS3 . Two bands of ~200 and 120 bp are expected to be cleaved from the 318 bp PCR products of ChrUScaf1915 . Two bands of ~200 and ~130 bp bands are expected to be cleaved from the 328 bp PCR products of dynamic-3-like . However, there is no appreciable off-target cleavage in the samples tested. M , 100 bp ladder markers.

    Journal: International Journal of Molecular Sciences

    Article Title: Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations

    doi: 10.3390/ijms160817838

    Figure Lengend Snippet: T7E1 analyses of three potential off-target sites (zinc finger protein GLIS3 , ChrUScaf1915 , and dynamin-3-like ) in blastocysts no. 1 (lane 1 ), 2 (lane 2 ), and 10 (lane 3 ) together with normal PEFs (lane C ). Two bands (~140 and ~80 bp) are expected to be cleaved from the 220 bp PCR products of GLIS3 . Two bands of ~200 and 120 bp are expected to be cleaved from the 318 bp PCR products of ChrUScaf1915 . Two bands of ~200 and ~130 bp bands are expected to be cleaved from the 328 bp PCR products of dynamic-3-like . However, there is no appreciable off-target cleavage in the samples tested. M , 100 bp ladder markers.

    Article Snippet: For the T7E1-based cleavage assay, 10 μl of 1× NEB2 reaction buffer (New England BioLabs Japan Inc., Tokyo, Japan) containing 400 ng of the nested PCR products derived from the experimental sample (200 ng; derived from a blastocyst developed from a mRNA-injected oocyte) and the control sample (200 ng; derived from normal PEFs) were placed in a 0.5-mL PCR tube (AxyGen Scientific, Inc.).

    Techniques: Polymerase Chain Reaction

    Upper panel . Production of ~350 bp products after first and nested PCR of genomic DNA initially amplified using WGA from a single blastocyst. Lanes 1 – 17 correspond to single numbered blastocysts (a part of these blastocysts is shown in Figure 2 ). The symbols above each lane indicate the fluorescence distribution type (ubiquitous (u) or mosaic (m)) and its strength (strong (++), moderate (+), slight (+/−), or none (−)); Middle panel : A T7E1-based assay for each single blastocyst. The approximately 350 bp PCR products shown in the upper panel were mixed with control (C) DNA at a ratio of 1:1, denatured, re-annealed, and then incubated with the T7E1 enzyme for 1 h at 37 °C. The resulting products were then electrophoresed in a 2% agarose gel. If samples have indel mutations, two fragments, namely ~200 and ~150 bp, were generated as cleaved products of the original ~350 bp products. The blue dots above the lanes indicate samples with mutations. Arrowheads indicate the position of cleaved bands, namely ~200 and 150 bp bands. Lanes 1 – 17 correspond to single numbered blastocysts as shown in the upper panel (except blastocyst number 13, which DNA amplification failed). Lane C indicates genomic DNA from normal PEFs used as a negative control; Lower panel : Production of 95 bp products after nested PCR of the first PCR products shown in the upper panel using primer sets N-S and D-RV (see Figure S1 and Table S1 ). Lanes 1 – 4 and 10 (all of which were identified as having mutations) correspond to single numbered blastocysts as shown in the upper and middle panels. Lane C indicates genomic DNA from normal porcine embryonic fibroblasts (PEFs) used as a positive control. M , 100 bp ladder markers.

    Journal: International Journal of Molecular Sciences

    Article Title: Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations

    doi: 10.3390/ijms160817838

    Figure Lengend Snippet: Upper panel . Production of ~350 bp products after first and nested PCR of genomic DNA initially amplified using WGA from a single blastocyst. Lanes 1 – 17 correspond to single numbered blastocysts (a part of these blastocysts is shown in Figure 2 ). The symbols above each lane indicate the fluorescence distribution type (ubiquitous (u) or mosaic (m)) and its strength (strong (++), moderate (+), slight (+/−), or none (−)); Middle panel : A T7E1-based assay for each single blastocyst. The approximately 350 bp PCR products shown in the upper panel were mixed with control (C) DNA at a ratio of 1:1, denatured, re-annealed, and then incubated with the T7E1 enzyme for 1 h at 37 °C. The resulting products were then electrophoresed in a 2% agarose gel. If samples have indel mutations, two fragments, namely ~200 and ~150 bp, were generated as cleaved products of the original ~350 bp products. The blue dots above the lanes indicate samples with mutations. Arrowheads indicate the position of cleaved bands, namely ~200 and 150 bp bands. Lanes 1 – 17 correspond to single numbered blastocysts as shown in the upper panel (except blastocyst number 13, which DNA amplification failed). Lane C indicates genomic DNA from normal PEFs used as a negative control; Lower panel : Production of 95 bp products after nested PCR of the first PCR products shown in the upper panel using primer sets N-S and D-RV (see Figure S1 and Table S1 ). Lanes 1 – 4 and 10 (all of which were identified as having mutations) correspond to single numbered blastocysts as shown in the upper and middle panels. Lane C indicates genomic DNA from normal porcine embryonic fibroblasts (PEFs) used as a positive control. M , 100 bp ladder markers.

    Article Snippet: For the T7E1-based cleavage assay, 10 μl of 1× NEB2 reaction buffer (New England BioLabs Japan Inc., Tokyo, Japan) containing 400 ng of the nested PCR products derived from the experimental sample (200 ng; derived from a blastocyst developed from a mRNA-injected oocyte) and the control sample (200 ng; derived from normal PEFs) were placed in a 0.5-mL PCR tube (AxyGen Scientific, Inc.).

    Techniques: Nested PCR, Amplification, Whole Genome Amplification, Fluorescence, Polymerase Chain Reaction, Incubation, Agarose Gel Electrophoresis, Generated, Negative Control, Positive Control

    Endpoint titration of 127S prions by a single round of mb-PMCA. Brain homogenate from tg338 mice infected with 127S prions was serially diluted in tg338 healthy brain lysate as indicated. Each dilution was directly analyzed by Western blotting (a) or served as seed for a single 48-h round of PMCA using different experimental conditions (b and c). Samples were amplified in the absence or presence of 3 ceramic beads (as indicated) in individual PCR tubes or an 8-PCR-tube strip containing 100 µl of PMCA mixtures (b) or in a PCR microplate containing 36 µl of PMCA mixture (c). Unseeded samples (U) were run on the same microplate. All samples were digested with PK before Western blot analysis using Sha31 anti-PrP antibody. Undigested normal brain homogenate (PrP C ) and PK-digested, infected brain homogenate (PrP res ) are provided as electrophoretic references. Molecular mass markers (kDa) are indicated to the left of each panel.

    Journal: mBio

    Article Title: Highly Infectious Prions Generated by a Single Round of Microplate-Based Protein Misfolding Cyclic Amplification

    doi: 10.1128/mBio.00829-13

    Figure Lengend Snippet: Endpoint titration of 127S prions by a single round of mb-PMCA. Brain homogenate from tg338 mice infected with 127S prions was serially diluted in tg338 healthy brain lysate as indicated. Each dilution was directly analyzed by Western blotting (a) or served as seed for a single 48-h round of PMCA using different experimental conditions (b and c). Samples were amplified in the absence or presence of 3 ceramic beads (as indicated) in individual PCR tubes or an 8-PCR-tube strip containing 100 µl of PMCA mixtures (b) or in a PCR microplate containing 36 µl of PMCA mixture (c). Unseeded samples (U) were run on the same microplate. All samples were digested with PK before Western blot analysis using Sha31 anti-PrP antibody. Undigested normal brain homogenate (PrP C ) and PK-digested, infected brain homogenate (PrP res ) are provided as electrophoretic references. Molecular mass markers (kDa) are indicated to the left of each panel.

    Article Snippet: PMCA was performed in a final volume of either 100 µl or 36 µl of lysate per well, in either single PCR tubes (conventional method); in 2-, 4-, or 8-PCR-tube strips; or with a 96-well PCR microplate (Axygen, Union City, CA, USA).

    Techniques: Titration, Mouse Assay, Infection, Western Blot, Amplification, Polymerase Chain Reaction, Stripping Membranes

    Application of HBP-rVAP polyclonal antiserum in immunodiagnostics. a Specific decoration of bacilliform particles of CYMV in immunosorbent electron microscopy (ISEM) using the HBP-rVAP antiserum (1:100 dilution). b Immunocapture PCR (IC-PCR) using RT/RNaseH primers. CYMV-infected citrus samples trapped with HBp-rVAP antiserum. Lane M–O′ generuler ladder mix (Thermo Scientific, USA). Lanes: 1–3 at dilutions of 1:250, 4–6 at dilutions 1: 500, and 7–9 at dilutions 1:1000, respectively. Lanes 1, 4 and 7—amplification of CYMV specific genome of amplicon size ~ 589 bp. Lanes 2, 5, and 8—showed faint amplification of genome size ~ 589 bp. Lanes 3, 6 and 9—showed no amplification in healthy control

    Journal: 3 Biotech

    Article Title: Efficient immunodiagnosis of Citrus yellow mosaic virus using polyclonal antibodies with an expressed recombinant virion-associated protein

    doi: 10.1007/s13205-017-1063-4

    Figure Lengend Snippet: Application of HBP-rVAP polyclonal antiserum in immunodiagnostics. a Specific decoration of bacilliform particles of CYMV in immunosorbent electron microscopy (ISEM) using the HBP-rVAP antiserum (1:100 dilution). b Immunocapture PCR (IC-PCR) using RT/RNaseH primers. CYMV-infected citrus samples trapped with HBp-rVAP antiserum. Lane M–O′ generuler ladder mix (Thermo Scientific, USA). Lanes: 1–3 at dilutions of 1:250, 4–6 at dilutions 1: 500, and 7–9 at dilutions 1:1000, respectively. Lanes 1, 4 and 7—amplification of CYMV specific genome of amplicon size ~ 589 bp. Lanes 2, 5, and 8—showed faint amplification of genome size ~ 589 bp. Lanes 3, 6 and 9—showed no amplification in healthy control

    Article Snippet: Different dilutions (1:250, 1:500 and 1:1000) of the purified polyclonal antiserum raised against CYMV-rVAP was coated (25 µl) on 0.2 ml sterile polypropylene PCR tubes (Axygen, Union city, USA) and kept overnight at 4 °C.

    Techniques: Electron Microscopy, Polymerase Chain Reaction, Infection, Amplification