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Bioneer Corporation polymerase chain reaction pcr premix
Polymerase Chain Reaction Pcr Premix, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr premix/product/Bioneer Corporation
Average 94 stars, based on 4 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr premix - by Bioz Stars, 2020-04
94/100 stars

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MTT Assay:

Article Title: Comparison of anticancer activities of Korean Red Ginseng-derived fractions
Article Snippet: Annexin V-FITC, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI), and staurosporine were purchased from Sigma Chemical Co. (St Louis, MO, USA). .. Polymerase chain reaction (PCR) premix and the primers used for semiquantitative reverse transcription PCR (RT-PCR) were obtained from Bioneer Inc. (Daejeon, Korea).

RNA Extraction:

Article Title: Effects of ChondroT on potassium Oxonate-induced Hyperuricemic mice: downregulation of xanthine oxidase and urate transporter 1
Article Snippet: Total RNA was isolated from the mouse kidney cortex tissue using an easy-BLUE total RNA extraction kit (iNtRON Biotechnology, Seongnam, Republic of Korea) according to the manufacturer’s instructions. .. Subsequently, the cDNA was amplified with gene-specific primers using the polymerase chain reaction (PCR) PreMix (Bioneer, Daejeon, Republic of Korea) according to the manufacturer’s instructions.

Amplification:

Article Title: Effects of ChondroT on potassium Oxonate-induced Hyperuricemic mice: downregulation of xanthine oxidase and urate transporter 1
Article Snippet: .. Subsequently, the cDNA was amplified with gene-specific primers using the polymerase chain reaction (PCR) PreMix (Bioneer, Daejeon, Republic of Korea) according to the manufacturer’s instructions. .. RT-PCR analysis were prepared according to previously described method [ , ], with modifications.

Polymerase Chain Reaction:

Article Title: Effects of Bevacizumab on Bcl-2 Expression and Apoptosis in Retinal Pigment Epithelial Cells under Oxidative Stress
Article Snippet: .. Preformed cDNA, Bcl-2 primer, and glycerol-3-phosphate dehydrogenase (GAPDH) primer were mixed with polymerase chain reaction (PCR) premix (Bioneer), and PCR was performed. ..

Article Title: Effects of ChondroT on potassium Oxonate-induced Hyperuricemic mice: downregulation of xanthine oxidase and urate transporter 1
Article Snippet: .. Subsequently, the cDNA was amplified with gene-specific primers using the polymerase chain reaction (PCR) PreMix (Bioneer, Daejeon, Republic of Korea) according to the manufacturer’s instructions. .. RT-PCR analysis were prepared according to previously described method [ , ], with modifications.

Article Title: Comparison of anticancer activities of Korean Red Ginseng-derived fractions
Article Snippet: .. Polymerase chain reaction (PCR) premix and the primers used for semiquantitative reverse transcription PCR (RT-PCR) were obtained from Bioneer Inc. (Daejeon, Korea). ..

Isolation:

Article Title: Effects of Bevacizumab on Bcl-2 Expression and Apoptosis in Retinal Pigment Epithelial Cells under Oxidative Stress
Article Snippet: Isolated RNA was quantified, and 1 µg of total RNA and 100 pmol of oligo dT were added to the reverse transcription (RT) premix (Bioneer, Seoul, Korea) to prepare 20 µL of cDNA. .. Preformed cDNA, Bcl-2 primer, and glycerol-3-phosphate dehydrogenase (GAPDH) primer were mixed with polymerase chain reaction (PCR) premix (Bioneer), and PCR was performed.

Article Title: Effects of ChondroT on potassium Oxonate-induced Hyperuricemic mice: downregulation of xanthine oxidase and urate transporter 1
Article Snippet: Total RNA was isolated from the mouse kidney cortex tissue using an easy-BLUE total RNA extraction kit (iNtRON Biotechnology, Seongnam, Republic of Korea) according to the manufacturer’s instructions. .. Subsequently, the cDNA was amplified with gene-specific primers using the polymerase chain reaction (PCR) PreMix (Bioneer, Daejeon, Republic of Korea) according to the manufacturer’s instructions.

Purification:

Article Title: Effects of Bevacizumab on Bcl-2 Expression and Apoptosis in Retinal Pigment Epithelial Cells under Oxidative Stress
Article Snippet: Total RNA was isolated using the total RNA purification kit (Invitrogen). .. Preformed cDNA, Bcl-2 primer, and glycerol-3-phosphate dehydrogenase (GAPDH) primer were mixed with polymerase chain reaction (PCR) premix (Bioneer), and PCR was performed.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Effects of Bevacizumab on Bcl-2 Expression and Apoptosis in Retinal Pigment Epithelial Cells under Oxidative Stress
Article Snippet: Paragraph title: Reverse transcription polymerase chain reaction ... Preformed cDNA, Bcl-2 primer, and glycerol-3-phosphate dehydrogenase (GAPDH) primer were mixed with polymerase chain reaction (PCR) premix (Bioneer), and PCR was performed.

Article Title: Effects of ChondroT on potassium Oxonate-induced Hyperuricemic mice: downregulation of xanthine oxidase and urate transporter 1
Article Snippet: Paragraph title: Reverse transcription-polymerase chain reaction (RT-PCR) ... Subsequently, the cDNA was amplified with gene-specific primers using the polymerase chain reaction (PCR) PreMix (Bioneer, Daejeon, Republic of Korea) according to the manufacturer’s instructions.

Article Title: Comparison of anticancer activities of Korean Red Ginseng-derived fractions
Article Snippet: .. Polymerase chain reaction (PCR) premix and the primers used for semiquantitative reverse transcription PCR (RT-PCR) were obtained from Bioneer Inc. (Daejeon, Korea). ..

Incubation:

Article Title: Effects of Bevacizumab on Bcl-2 Expression and Apoptosis in Retinal Pigment Epithelial Cells under Oxidative Stress
Article Snippet: H2 O2 and bevacizumab were challenged at different concentrations (0, 100, 200, 300, 400 µM and 0.33, 0.67, 1.33, 2.67 mg/mL) and incubated for 16 hours. .. Preformed cDNA, Bcl-2 primer, and glycerol-3-phosphate dehydrogenase (GAPDH) primer were mixed with polymerase chain reaction (PCR) premix (Bioneer), and PCR was performed.

Article Title: Effects of ChondroT on potassium Oxonate-induced Hyperuricemic mice: downregulation of xanthine oxidase and urate transporter 1
Article Snippet: To synthesize the cDNA, 1 μg total RNA was mixed with a premix containing the oligo (dT) primer and diethyl pyrocarbonate-treated water in a final volume of 20 μL and incubated at 45 °C for 60 min. .. Subsequently, the cDNA was amplified with gene-specific primers using the polymerase chain reaction (PCR) PreMix (Bioneer, Daejeon, Republic of Korea) according to the manufacturer’s instructions.

Modification:

Article Title: Comparison of anticancer activities of Korean Red Ginseng-derived fractions
Article Snippet: Dulbecco's Modified Eagle's medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), streptomycin, penicillin, and L-glutamine were purchased from Gibco (Grand Island, NY, USA). .. Polymerase chain reaction (PCR) premix and the primers used for semiquantitative reverse transcription PCR (RT-PCR) were obtained from Bioneer Inc. (Daejeon, Korea).

Mouse Assay:

Article Title: Comparison of anticancer activities of Korean Red Ginseng-derived fractions
Article Snippet: Male Balb/c mice (age, 6–8 wk; weight, 17–21 g) were purchased from Orient Bio (Gyeonggi, Korea). .. Polymerase chain reaction (PCR) premix and the primers used for semiquantitative reverse transcription PCR (RT-PCR) were obtained from Bioneer Inc. (Daejeon, Korea).

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  • 99
    Bioneer Corporation accupower pcr premix
    Effects of different concentrations and sizes of UCNPs on <t>PCR</t> amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) <t>AccuPower</t> PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Accupower Pcr Premix, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 99/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accupower pcr premix/product/Bioneer Corporation
    Average 99 stars, based on 303 article reviews
    Price from $9.99 to $1999.99
    accupower pcr premix - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    95
    Bioneer Corporation pcr premix
    There are three functional ARE cis -elements in the human <t>NDP52</t> promoter ( a ) Diagram shows five putative ARE cis -acting elements in human NDP52 promoter region (2,137 bps). Red ellipsoids indicate the putative ARE cis -acting elements. ( b, c, d ) The mutant forms of pGL4.14-Luc reporter plasmid with the human NDP52 promoter gene were prepared by digestion with specific restriction enzymes (e.g., Hind III, Sma I or Eco RI), by cloning of specific regions following <t>PCR</t> amplification, or with the QuikChange site-directed mutagenesis kit (Agilent Technologies). ( b ) CN1.4 cortical neurons and SH-SY5Y cells were transiently transfected with the reporter plasmids with the human NDP52 promoter (2,317 bps or deleted mutants), and luciferase activity assayed 24 h after transfection. ( c ) SH-SY5Y cells were co-transfected with the reporter plasmids with human NDP52 promoter (wild or deleted mutants) along with either the mock plasmid (pcDNA3.1) or the plasmid expressing Nrf2, and the luciferase activity was assayed 24 h after transfection. ( d ) SH-SY5Y cells were transiently co-transfected with the reporter plasmids with human NDP52 promoter or its mutant plasmid in which the putative ARE sequence was site-directly mutated (X), together with the mock plasmid (pcDNA3.1) or the plasmid expressing human Nrf2. The luciferase activity was assayed after 24 h. The induced luciferase activity with co-expression with Nrf2 was not observed in a mutant NDP52 promoter in which all three ARE sites (−2057, −1023 and −94) were site-directly mutated, indicating these three sites of five ARE cis -acting elements are functional. Data shown are mean±SD of three independent experiments and were analyzed using Student’s t test. (*, p
    Pcr Premix, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 95/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr premix/product/Bioneer Corporation
    Average 95 stars, based on 113 article reviews
    Price from $9.99 to $1999.99
    pcr premix - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    92
    Bioneer Corporation gold multiplex pcr premix
    Agarose gel electrophoresis of <t>DNA</t> amplified from various genomic DNA extracts of Korean native honey (KNH) and European honey (EH) in duplex <t>PCR</t> with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.
    Gold Multiplex Pcr Premix, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gold multiplex pcr premix/product/Bioneer Corporation
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gold multiplex pcr premix - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Journal: PLoS ONE

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction

    doi: 10.1371/journal.pone.0073408

    Figure Lengend Snippet: Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Article Snippet: Top DNA polymerase, used in the AccuPower PCR PreMix, was a modified form of a recombinant DNA polymerase, originally isolated from Thermus thermophilus .

    Techniques: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Marker

    Determination of the detection limit of conventional PCR using AccuPower™ PCR PreMix system. Four µl of PCR products were loaded on 1% agarose gels and ethidium bromide-stained specific PCR bands were visualized using a UV-transilluminator. PCR was carried out using various concentrations of the full-length 18S rRNA gene-harboring plasmid pKitauei (A), the suspension containing different number of spores (B), genomic DNA purified from isolated spores (C) and genomic DNA from the various numbers of spore-spiked tissues (D) as templates. M: 100 bp ladder from Bioneer.co.kr

    Journal: The Korean Journal of Parasitology

    Article Title: Molecular Identification and Real-time Quantitative PCR (qPCR) for Rapid Detection of Thelohanellus kitauei, a Myxozoan Parasite Causing Intestinal Giant Cystic Disease in the Israel Carp

    doi: 10.3347/kjp.2012.50.2.103

    Figure Lengend Snippet: Determination of the detection limit of conventional PCR using AccuPower™ PCR PreMix system. Four µl of PCR products were loaded on 1% agarose gels and ethidium bromide-stained specific PCR bands were visualized using a UV-transilluminator. PCR was carried out using various concentrations of the full-length 18S rRNA gene-harboring plasmid pKitauei (A), the suspension containing different number of spores (B), genomic DNA purified from isolated spores (C) and genomic DNA from the various numbers of spore-spiked tissues (D) as templates. M: 100 bp ladder from Bioneer.co.kr

    Article Snippet: Conventional PCR was conducted using AccuPower™ PCR PreMix (Bioneer, Seoul, Korea) for the common laboratory experiments, and real-time qPCR was carried out using the FastStart Universal SYBR Green Master (Roche) for quantification of the infection intensity.

    Techniques: Polymerase Chain Reaction, Staining, Plasmid Preparation, Purification, Isolation

    There are three functional ARE cis -elements in the human NDP52 promoter ( a ) Diagram shows five putative ARE cis -acting elements in human NDP52 promoter region (2,137 bps). Red ellipsoids indicate the putative ARE cis -acting elements. ( b, c, d ) The mutant forms of pGL4.14-Luc reporter plasmid with the human NDP52 promoter gene were prepared by digestion with specific restriction enzymes (e.g., Hind III, Sma I or Eco RI), by cloning of specific regions following PCR amplification, or with the QuikChange site-directed mutagenesis kit (Agilent Technologies). ( b ) CN1.4 cortical neurons and SH-SY5Y cells were transiently transfected with the reporter plasmids with the human NDP52 promoter (2,317 bps or deleted mutants), and luciferase activity assayed 24 h after transfection. ( c ) SH-SY5Y cells were co-transfected with the reporter plasmids with human NDP52 promoter (wild or deleted mutants) along with either the mock plasmid (pcDNA3.1) or the plasmid expressing Nrf2, and the luciferase activity was assayed 24 h after transfection. ( d ) SH-SY5Y cells were transiently co-transfected with the reporter plasmids with human NDP52 promoter or its mutant plasmid in which the putative ARE sequence was site-directly mutated (X), together with the mock plasmid (pcDNA3.1) or the plasmid expressing human Nrf2. The luciferase activity was assayed after 24 h. The induced luciferase activity with co-expression with Nrf2 was not observed in a mutant NDP52 promoter in which all three ARE sites (−2057, −1023 and −94) were site-directly mutated, indicating these three sites of five ARE cis -acting elements are functional. Data shown are mean±SD of three independent experiments and were analyzed using Student’s t test. (*, p

    Journal: Nature communications

    Article Title: Nrf2 reduces levels of phosphorylated tau protein by inducing autophagy adaptor protein NDP52

    doi: 10.1038/ncomms4496

    Figure Lengend Snippet: There are three functional ARE cis -elements in the human NDP52 promoter ( a ) Diagram shows five putative ARE cis -acting elements in human NDP52 promoter region (2,137 bps). Red ellipsoids indicate the putative ARE cis -acting elements. ( b, c, d ) The mutant forms of pGL4.14-Luc reporter plasmid with the human NDP52 promoter gene were prepared by digestion with specific restriction enzymes (e.g., Hind III, Sma I or Eco RI), by cloning of specific regions following PCR amplification, or with the QuikChange site-directed mutagenesis kit (Agilent Technologies). ( b ) CN1.4 cortical neurons and SH-SY5Y cells were transiently transfected with the reporter plasmids with the human NDP52 promoter (2,317 bps or deleted mutants), and luciferase activity assayed 24 h after transfection. ( c ) SH-SY5Y cells were co-transfected with the reporter plasmids with human NDP52 promoter (wild or deleted mutants) along with either the mock plasmid (pcDNA3.1) or the plasmid expressing Nrf2, and the luciferase activity was assayed 24 h after transfection. ( d ) SH-SY5Y cells were transiently co-transfected with the reporter plasmids with human NDP52 promoter or its mutant plasmid in which the putative ARE sequence was site-directly mutated (X), together with the mock plasmid (pcDNA3.1) or the plasmid expressing human Nrf2. The luciferase activity was assayed after 24 h. The induced luciferase activity with co-expression with Nrf2 was not observed in a mutant NDP52 promoter in which all three ARE sites (−2057, −1023 and −94) were site-directly mutated, indicating these three sites of five ARE cis -acting elements are functional. Data shown are mean±SD of three independent experiments and were analyzed using Student’s t test. (*, p

    Article Snippet: Mouse NDP52 gene was amplified using the PCR premix (Bioneer, Korea) following addition of 1 µL of cDNA product and 1 µM of primers (forward and reverse, ) on a PCR machine (Applied Biosystems).

    Techniques: Functional Assay, Mutagenesis, Plasmid Preparation, Clone Assay, Polymerase Chain Reaction, Amplification, Transfection, Luciferase, Activity Assay, Expressing, Sequencing

    NDP52 is induced by Nrf2 activation and its expression is significantly reduced in Nrf2 (−/−) mice ( a ) Primary cortical neurons (DIV 5) were treated with sulforaphane (SFN, 10 µM) for 12 h, and the relative mRNA level of each gene was compared to that of cells not treated with SFN using qRT-PCR. ( b ) In hippocampal tissues from Nrf2 (−/−) (5 months old, 1 male; 11 months old 1 female; 5 months old, 1 female; 12 months old, 3 female) or wild-type (10 months old, 4 male; 2 female) mice, the relative mRNA level of each gene compared with that of wild-type mice. n=6. ( c ) The levels of p62/SQSTM1, Keap1, mouse NDP52 (mNDP52) and Hsp70 in Nrf2 (−/−) or wild-type mice were examined by immunoblotting. The asterisk on the Nrf2 panel indicates a non-specific band. ( d, e : upper panel) Primary cortical neurons (DIV 5) ( d ) and SH-SY5Y cells ( e ) were treated with SFN for 24 h, and the expression levels of rat and human NDP52 (rNDP52 and hNDP52) examined by immunoblotting using anti-NDP52 antibody. The relative molecular masses (kD) are indicated to the left of each blot. ( d, e : low panel) Primary cortical neurons (DIV 5) ( d ) and SH-SY5Y cells ( e ) transiently transfected with the pGL4.14/human NDP52 promoter (2,173 bps) luciferase reporter plasmid were treated with SFN for 24 h, and assayed for the luciferase activity. ( f ) SH-SY5Y cells were treated with SFN for 12 h, and cell lysates were used for the ChIP assay using anti-Nrf2 antibody as described in the Methods. Data shown are mean±SE and were analyzed using Student’s t test. (*, p

    Journal: Nature communications

    Article Title: Nrf2 reduces levels of phosphorylated tau protein by inducing autophagy adaptor protein NDP52

    doi: 10.1038/ncomms4496

    Figure Lengend Snippet: NDP52 is induced by Nrf2 activation and its expression is significantly reduced in Nrf2 (−/−) mice ( a ) Primary cortical neurons (DIV 5) were treated with sulforaphane (SFN, 10 µM) for 12 h, and the relative mRNA level of each gene was compared to that of cells not treated with SFN using qRT-PCR. ( b ) In hippocampal tissues from Nrf2 (−/−) (5 months old, 1 male; 11 months old 1 female; 5 months old, 1 female; 12 months old, 3 female) or wild-type (10 months old, 4 male; 2 female) mice, the relative mRNA level of each gene compared with that of wild-type mice. n=6. ( c ) The levels of p62/SQSTM1, Keap1, mouse NDP52 (mNDP52) and Hsp70 in Nrf2 (−/−) or wild-type mice were examined by immunoblotting. The asterisk on the Nrf2 panel indicates a non-specific band. ( d, e : upper panel) Primary cortical neurons (DIV 5) ( d ) and SH-SY5Y cells ( e ) were treated with SFN for 24 h, and the expression levels of rat and human NDP52 (rNDP52 and hNDP52) examined by immunoblotting using anti-NDP52 antibody. The relative molecular masses (kD) are indicated to the left of each blot. ( d, e : low panel) Primary cortical neurons (DIV 5) ( d ) and SH-SY5Y cells ( e ) transiently transfected with the pGL4.14/human NDP52 promoter (2,173 bps) luciferase reporter plasmid were treated with SFN for 24 h, and assayed for the luciferase activity. ( f ) SH-SY5Y cells were treated with SFN for 12 h, and cell lysates were used for the ChIP assay using anti-Nrf2 antibody as described in the Methods. Data shown are mean±SE and were analyzed using Student’s t test. (*, p

    Article Snippet: Mouse NDP52 gene was amplified using the PCR premix (Bioneer, Korea) following addition of 1 µL of cDNA product and 1 µM of primers (forward and reverse, ) on a PCR machine (Applied Biosystems).

    Techniques: Activation Assay, Expressing, Mouse Assay, Quantitative RT-PCR, Transfection, Luciferase, Plasmid Preparation, Activity Assay, Chromatin Immunoprecipitation

    Agarose gel electrophoresis of DNA amplified from various genomic DNA extracts of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA amplified from various genomic DNA extracts of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.

    Article Snippet: Accuprep genomic DNA purification kit, Gold multiplex PCR premix, and primer oligonucleotides (Bioneer, Korea), Wizard genomic DNA purification kit (Promega, USA), Chelex 100 (Bio-Rad, USA), NuSieve 3:1 agarose (Lonza, USA), monoclonal anti-TAMRA antibody, rabbit antidigoxigenin antibody (Abscam, USA), streptavidine (NE Biolab, USA), and gold nanoparticles (Rapigen, Korea) were used in the study.

    Techniques: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction

    Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M5-F and M5-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M5-F and M5-R.

    Article Snippet: Accuprep genomic DNA purification kit, Gold multiplex PCR premix, and primer oligonucleotides (Bioneer, Korea), Wizard genomic DNA purification kit (Promega, USA), Chelex 100 (Bio-Rad, USA), NuSieve 3:1 agarose (Lonza, USA), monoclonal anti-TAMRA antibody, rabbit antidigoxigenin antibody (Abscam, USA), streptavidine (NE Biolab, USA), and gold nanoparticles (Rapigen, Korea) were used in the study.

    Techniques: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction

    Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of ten Korean native honey (KNH) products in duplex PCR with the KNH- specific primers, C6-F and C5-R and the European honey-specific primers, M5-F and M5-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of ten Korean native honey (KNH) products in duplex PCR with the KNH- specific primers, C6-F and C5-R and the European honey-specific primers, M5-F and M5-R.

    Article Snippet: Accuprep genomic DNA purification kit, Gold multiplex PCR premix, and primer oligonucleotides (Bioneer, Korea), Wizard genomic DNA purification kit (Promega, USA), Chelex 100 (Bio-Rad, USA), NuSieve 3:1 agarose (Lonza, USA), monoclonal anti-TAMRA antibody, rabbit antidigoxigenin antibody (Abscam, USA), streptavidine (NE Biolab, USA), and gold nanoparticles (Rapigen, Korea) were used in the study.

    Techniques: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction

    Immunochromatographic assay of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R and the EH-specific primers, M5-F and M5-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Immunochromatographic assay of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R and the EH-specific primers, M5-F and M5-R.

    Article Snippet: Accuprep genomic DNA purification kit, Gold multiplex PCR premix, and primer oligonucleotides (Bioneer, Korea), Wizard genomic DNA purification kit (Promega, USA), Chelex 100 (Bio-Rad, USA), NuSieve 3:1 agarose (Lonza, USA), monoclonal anti-TAMRA antibody, rabbit antidigoxigenin antibody (Abscam, USA), streptavidine (NE Biolab, USA), and gold nanoparticles (Rapigen, Korea) were used in the study.

    Techniques: Immunochromatographic Assay, Amplification, Polymerase Chain Reaction

    Immunochromatographic assay of DNA amplified from genomic DNA extracts of seven Korean native honey (KNH) products in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the European honey(EH)-specific primers, M5-F and M5-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Immunochromatographic assay of DNA amplified from genomic DNA extracts of seven Korean native honey (KNH) products in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the European honey(EH)-specific primers, M5-F and M5-R.

    Article Snippet: Accuprep genomic DNA purification kit, Gold multiplex PCR premix, and primer oligonucleotides (Bioneer, Korea), Wizard genomic DNA purification kit (Promega, USA), Chelex 100 (Bio-Rad, USA), NuSieve 3:1 agarose (Lonza, USA), monoclonal anti-TAMRA antibody, rabbit antidigoxigenin antibody (Abscam, USA), streptavidine (NE Biolab, USA), and gold nanoparticles (Rapigen, Korea) were used in the study.

    Techniques: Immunochromatographic Assay, Amplification, Polymerase Chain Reaction

    Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Detection of Korean Native Honey and European Honey by Using Duplex Polymerase Chain Reaction and Immunochromatographic Assay

    doi: 10.5851/kosfa.2017.37.4.599

    Figure Lengend Snippet: Agarose gel electrophoresis of DNA amplified from genomic DNA extracts of the mixtures of Korean native honey (KNH) and European honey (EH) in duplex PCR with the KNH-specific primers, C6-F and C5-R, and the EH-specific primers, M2-F and M2-R.

    Article Snippet: Accuprep genomic DNA purification kit, Gold multiplex PCR premix, and primer oligonucleotides (Bioneer, Korea), Wizard genomic DNA purification kit (Promega, USA), Chelex 100 (Bio-Rad, USA), NuSieve 3:1 agarose (Lonza, USA), monoclonal anti-TAMRA antibody, rabbit antidigoxigenin antibody (Abscam, USA), streptavidine (NE Biolab, USA), and gold nanoparticles (Rapigen, Korea) were used in the study.

    Techniques: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction