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tiangen biotech co polymerase chain reaction pcr mixture
Polymerase Chain Reaction Pcr Mixture, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr mixture/product/tiangen biotech co
Average 90 stars, based on 7 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr mixture - by Bioz Stars, 2020-08
90/100 stars

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Irradiation:

Article Title: Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma Cells
Article Snippet: .. Main reagents The main reagents were Opti-MEM (Thermo Fisher Scientific Inc., USA); polymerase chain reaction (PCR) mix (Tiangen Biotech (Beijing) CO., LTD.); real-time fluorescence quantitative PCR (RT-PCR) mixture and iTaq™ SYBR Green Supermix (Bio-Rad); β5i (LMP7) antibody (Santa Cruz Biotechnology); horseradish peroxidase (HRP) -labeled β-actin mouse monoclonal antibody (Millipore Sigma Canada Co.); HRP-labeled rabbit anti-goat antibody (Amersham Pharmacia Biotech); complementary DNA (cDNA) RT-PCR kit (Promega GoScript™ Reverse Transcription System); γ-ray irradiation source: 60 Co (Beijing Institute of Biotechnology, Beijing 100850, China); plasmid vectors pLKO.1-TRC (Addgene plasmid #10878; http://n2t.net/addgene: 10878; RRID: Addgene_10878), psPAX2, and pMD2. .. G (Invitrogen); DNA restriction endonucleases AgeI (NEB, #R0552S), EcoRI (NEB, #R0101S), and NcoI (NEB, #R3193), along with Buffer 2 (NEB, #B7002S) and T4 DNA Ligase (NEB, #M0202S); FuGENE HD transfection reagent (Roche); molecular weight standard: DNA Marker (DL2000, DL15000) (Tiangen Biotech (Beijing)); low-molecular-weight protein standard (PageRuler Prestained protein ladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction and purification kit (Tiangen Biotech, Beijing); the exogenous tyrosine kinase inhibitor (TKI) nilotinib (Novartis Pharma Stein AG); human recombinant H-IFN-γ (PeproTech, US), with a final concentration of 150 U/ml; c-Abl activator DPH (MedChemExpress, US), with a final concentration of 10 mM.

Amplification:

Article Title: Puerarin alleviates burn-related procedural pain mediated by P2X3 receptors
Article Snippet: .. It was reverse-transcribed into complementary DNA (cDNA) with Revert Aid First Strand cDNA Synthesis Kit (Fermentas Co.). cDNA was amplified in 50 μl of polymerase chain reaction (PCR) mixture (TIANGEN Co.). ..

Fluorescence:

Article Title: Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma Cells
Article Snippet: .. Main reagents The main reagents were Opti-MEM (Thermo Fisher Scientific Inc., USA); polymerase chain reaction (PCR) mix (Tiangen Biotech (Beijing) CO., LTD.); real-time fluorescence quantitative PCR (RT-PCR) mixture and iTaq™ SYBR Green Supermix (Bio-Rad); β5i (LMP7) antibody (Santa Cruz Biotechnology); horseradish peroxidase (HRP) -labeled β-actin mouse monoclonal antibody (Millipore Sigma Canada Co.); HRP-labeled rabbit anti-goat antibody (Amersham Pharmacia Biotech); complementary DNA (cDNA) RT-PCR kit (Promega GoScript™ Reverse Transcription System); γ-ray irradiation source: 60 Co (Beijing Institute of Biotechnology, Beijing 100850, China); plasmid vectors pLKO.1-TRC (Addgene plasmid #10878; http://n2t.net/addgene: 10878; RRID: Addgene_10878), psPAX2, and pMD2. .. G (Invitrogen); DNA restriction endonucleases AgeI (NEB, #R0552S), EcoRI (NEB, #R0101S), and NcoI (NEB, #R3193), along with Buffer 2 (NEB, #B7002S) and T4 DNA Ligase (NEB, #M0202S); FuGENE HD transfection reagent (Roche); molecular weight standard: DNA Marker (DL2000, DL15000) (Tiangen Biotech (Beijing)); low-molecular-weight protein standard (PageRuler Prestained protein ladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction and purification kit (Tiangen Biotech, Beijing); the exogenous tyrosine kinase inhibitor (TKI) nilotinib (Novartis Pharma Stein AG); human recombinant H-IFN-γ (PeproTech, US), with a final concentration of 150 U/ml; c-Abl activator DPH (MedChemExpress, US), with a final concentration of 10 mM.

Labeling:

Article Title: Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma Cells
Article Snippet: .. Main reagents The main reagents were Opti-MEM (Thermo Fisher Scientific Inc., USA); polymerase chain reaction (PCR) mix (Tiangen Biotech (Beijing) CO., LTD.); real-time fluorescence quantitative PCR (RT-PCR) mixture and iTaq™ SYBR Green Supermix (Bio-Rad); β5i (LMP7) antibody (Santa Cruz Biotechnology); horseradish peroxidase (HRP) -labeled β-actin mouse monoclonal antibody (Millipore Sigma Canada Co.); HRP-labeled rabbit anti-goat antibody (Amersham Pharmacia Biotech); complementary DNA (cDNA) RT-PCR kit (Promega GoScript™ Reverse Transcription System); γ-ray irradiation source: 60 Co (Beijing Institute of Biotechnology, Beijing 100850, China); plasmid vectors pLKO.1-TRC (Addgene plasmid #10878; http://n2t.net/addgene: 10878; RRID: Addgene_10878), psPAX2, and pMD2. .. G (Invitrogen); DNA restriction endonucleases AgeI (NEB, #R0552S), EcoRI (NEB, #R0101S), and NcoI (NEB, #R3193), along with Buffer 2 (NEB, #B7002S) and T4 DNA Ligase (NEB, #M0202S); FuGENE HD transfection reagent (Roche); molecular weight standard: DNA Marker (DL2000, DL15000) (Tiangen Biotech (Beijing)); low-molecular-weight protein standard (PageRuler Prestained protein ladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction and purification kit (Tiangen Biotech, Beijing); the exogenous tyrosine kinase inhibitor (TKI) nilotinib (Novartis Pharma Stein AG); human recombinant H-IFN-γ (PeproTech, US), with a final concentration of 150 U/ml; c-Abl activator DPH (MedChemExpress, US), with a final concentration of 10 mM.

SYBR Green Assay:

Article Title: Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma Cells
Article Snippet: .. Main reagents The main reagents were Opti-MEM (Thermo Fisher Scientific Inc., USA); polymerase chain reaction (PCR) mix (Tiangen Biotech (Beijing) CO., LTD.); real-time fluorescence quantitative PCR (RT-PCR) mixture and iTaq™ SYBR Green Supermix (Bio-Rad); β5i (LMP7) antibody (Santa Cruz Biotechnology); horseradish peroxidase (HRP) -labeled β-actin mouse monoclonal antibody (Millipore Sigma Canada Co.); HRP-labeled rabbit anti-goat antibody (Amersham Pharmacia Biotech); complementary DNA (cDNA) RT-PCR kit (Promega GoScript™ Reverse Transcription System); γ-ray irradiation source: 60 Co (Beijing Institute of Biotechnology, Beijing 100850, China); plasmid vectors pLKO.1-TRC (Addgene plasmid #10878; http://n2t.net/addgene: 10878; RRID: Addgene_10878), psPAX2, and pMD2. .. G (Invitrogen); DNA restriction endonucleases AgeI (NEB, #R0552S), EcoRI (NEB, #R0101S), and NcoI (NEB, #R3193), along with Buffer 2 (NEB, #B7002S) and T4 DNA Ligase (NEB, #M0202S); FuGENE HD transfection reagent (Roche); molecular weight standard: DNA Marker (DL2000, DL15000) (Tiangen Biotech (Beijing)); low-molecular-weight protein standard (PageRuler Prestained protein ladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction and purification kit (Tiangen Biotech, Beijing); the exogenous tyrosine kinase inhibitor (TKI) nilotinib (Novartis Pharma Stein AG); human recombinant H-IFN-γ (PeproTech, US), with a final concentration of 150 U/ml; c-Abl activator DPH (MedChemExpress, US), with a final concentration of 10 mM.

Polymerase Chain Reaction:

Article Title: Climate Is Not All: Evidence From Phylogeography of Rhodiola fastigiata (Crassulaceae) and Comparison to Its Closest Relatives
Article Snippet: .. Polymerase Chain Reaction (PCR) mixture were 20 μl with 2 μl 10× buffer, 0.5 μl of each primer, 0.4 μl of dNTP mixture, 1 U of Taq polymerase (TianGen Biotech, Xi’an, China) and 1 μl template genomic DNA. .. The PCR cycling programs followed .

Article Title: Correlation of MDR1 gene polymorphisms with anesthetic effect of sevoflurane–remifentanil following pediatric tonsillectomy
Article Snippet: .. The whole reaction system was 20 μL with extracted DNA of 1 μL, forward and reverse primer (10 pmol/mL) of 1 μL for each polymerase chain reaction (PCR) mixture of 10 μL, and deionized water of 7 μL (First Strand cDNA Synthesis Kit No., KP202-03; Tiangen Biotech Co. Ltd, Beijing, China). ..

Article Title: Puerarin alleviates burn-related procedural pain mediated by P2X3 receptors
Article Snippet: .. It was reverse-transcribed into complementary DNA (cDNA) with Revert Aid First Strand cDNA Synthesis Kit (Fermentas Co.). cDNA was amplified in 50 μl of polymerase chain reaction (PCR) mixture (TIANGEN Co.). ..

Article Title: Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.)
Article Snippet: .. The 25 μL polymerase chain reaction (PCR) mixture consisted of 100 ng of genomic DNA, 0.5 μL (25 μM) of each primer and 12.5 μL of 2 × Taq PCR Master Mix and 10.5 μL of ddH2 O (Tiangen, Beijing, China). ..

Article Title: Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet *
Article Snippet: .. The polymerase chain reaction (PCR) mixture (25 μl total volume) contained 12.5 μl of 2× Taq PCR master mix (Tiangen, Beijing, China), 8.5 μl of ddH2 O, 2 μl of mixed cDNA, and l μl (10 nmol/L) of each primer. .. The PCR reaction was performed using Eppendorf Mastercycler (Eppendorf, Hamburg, Germany) with the following parameters: initial denaturation at 94 °C for 5 min; 35 cycles of denaturation at 94 °C for 60 s, annealing at 55 °C for 60 s, and elongation at 72 °C for 60 s; and final elongation at 72 °C for 10 min. PCR products (4 μl) were identified by electrophoresis at 12 V/cm for 50 min on a 1% (0.01 g/ml) agarose gel.

Article Title: Significant Association Between CAV1 Variant rs3807989 on 7p31 and Atrial Fibrillation in a Chinese Han Population
Article Snippet: .. A total of 25 μL of polymerase chain reaction (PCR) mixture for genotyping contained 1.5 mmol/L of Mg2+ , 0.2 mmol/L of dNTPs, 0.5 μmol/L of each primer, 25 ng of human genomic DNA template, 5 μmol/L of SYTO 9 green fluorescent, and 0.15 U of Taq DNA polymerase (TIANGEN, Beijing, China). .. PCR was performed on an ABI 9700 System (Applied Biosystems, Foster City, CA) with a thermal profile of 95°C for 5 minutes, 40 cycles of 95°C for 10 seconds, 59.4°C or other appropriate annealing temperatures for 10 seconds and 72°C for 15 seconds, and 72°C for 10 minutes.

Article Title: Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma Cells
Article Snippet: .. Main reagents The main reagents were Opti-MEM (Thermo Fisher Scientific Inc., USA); polymerase chain reaction (PCR) mix (Tiangen Biotech (Beijing) CO., LTD.); real-time fluorescence quantitative PCR (RT-PCR) mixture and iTaq™ SYBR Green Supermix (Bio-Rad); β5i (LMP7) antibody (Santa Cruz Biotechnology); horseradish peroxidase (HRP) -labeled β-actin mouse monoclonal antibody (Millipore Sigma Canada Co.); HRP-labeled rabbit anti-goat antibody (Amersham Pharmacia Biotech); complementary DNA (cDNA) RT-PCR kit (Promega GoScript™ Reverse Transcription System); γ-ray irradiation source: 60 Co (Beijing Institute of Biotechnology, Beijing 100850, China); plasmid vectors pLKO.1-TRC (Addgene plasmid #10878; http://n2t.net/addgene: 10878; RRID: Addgene_10878), psPAX2, and pMD2. .. G (Invitrogen); DNA restriction endonucleases AgeI (NEB, #R0552S), EcoRI (NEB, #R0101S), and NcoI (NEB, #R3193), along with Buffer 2 (NEB, #B7002S) and T4 DNA Ligase (NEB, #M0202S); FuGENE HD transfection reagent (Roche); molecular weight standard: DNA Marker (DL2000, DL15000) (Tiangen Biotech (Beijing)); low-molecular-weight protein standard (PageRuler Prestained protein ladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction and purification kit (Tiangen Biotech, Beijing); the exogenous tyrosine kinase inhibitor (TKI) nilotinib (Novartis Pharma Stein AG); human recombinant H-IFN-γ (PeproTech, US), with a final concentration of 150 U/ml; c-Abl activator DPH (MedChemExpress, US), with a final concentration of 10 mM.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma Cells
Article Snippet: .. Main reagents The main reagents were Opti-MEM (Thermo Fisher Scientific Inc., USA); polymerase chain reaction (PCR) mix (Tiangen Biotech (Beijing) CO., LTD.); real-time fluorescence quantitative PCR (RT-PCR) mixture and iTaq™ SYBR Green Supermix (Bio-Rad); β5i (LMP7) antibody (Santa Cruz Biotechnology); horseradish peroxidase (HRP) -labeled β-actin mouse monoclonal antibody (Millipore Sigma Canada Co.); HRP-labeled rabbit anti-goat antibody (Amersham Pharmacia Biotech); complementary DNA (cDNA) RT-PCR kit (Promega GoScript™ Reverse Transcription System); γ-ray irradiation source: 60 Co (Beijing Institute of Biotechnology, Beijing 100850, China); plasmid vectors pLKO.1-TRC (Addgene plasmid #10878; http://n2t.net/addgene: 10878; RRID: Addgene_10878), psPAX2, and pMD2. .. G (Invitrogen); DNA restriction endonucleases AgeI (NEB, #R0552S), EcoRI (NEB, #R0101S), and NcoI (NEB, #R3193), along with Buffer 2 (NEB, #B7002S) and T4 DNA Ligase (NEB, #M0202S); FuGENE HD transfection reagent (Roche); molecular weight standard: DNA Marker (DL2000, DL15000) (Tiangen Biotech (Beijing)); low-molecular-weight protein standard (PageRuler Prestained protein ladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction and purification kit (Tiangen Biotech, Beijing); the exogenous tyrosine kinase inhibitor (TKI) nilotinib (Novartis Pharma Stein AG); human recombinant H-IFN-γ (PeproTech, US), with a final concentration of 150 U/ml; c-Abl activator DPH (MedChemExpress, US), with a final concentration of 10 mM.

Real-time Polymerase Chain Reaction:

Article Title: Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma Cells
Article Snippet: .. Main reagents The main reagents were Opti-MEM (Thermo Fisher Scientific Inc., USA); polymerase chain reaction (PCR) mix (Tiangen Biotech (Beijing) CO., LTD.); real-time fluorescence quantitative PCR (RT-PCR) mixture and iTaq™ SYBR Green Supermix (Bio-Rad); β5i (LMP7) antibody (Santa Cruz Biotechnology); horseradish peroxidase (HRP) -labeled β-actin mouse monoclonal antibody (Millipore Sigma Canada Co.); HRP-labeled rabbit anti-goat antibody (Amersham Pharmacia Biotech); complementary DNA (cDNA) RT-PCR kit (Promega GoScript™ Reverse Transcription System); γ-ray irradiation source: 60 Co (Beijing Institute of Biotechnology, Beijing 100850, China); plasmid vectors pLKO.1-TRC (Addgene plasmid #10878; http://n2t.net/addgene: 10878; RRID: Addgene_10878), psPAX2, and pMD2. .. G (Invitrogen); DNA restriction endonucleases AgeI (NEB, #R0552S), EcoRI (NEB, #R0101S), and NcoI (NEB, #R3193), along with Buffer 2 (NEB, #B7002S) and T4 DNA Ligase (NEB, #M0202S); FuGENE HD transfection reagent (Roche); molecular weight standard: DNA Marker (DL2000, DL15000) (Tiangen Biotech (Beijing)); low-molecular-weight protein standard (PageRuler Prestained protein ladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction and purification kit (Tiangen Biotech, Beijing); the exogenous tyrosine kinase inhibitor (TKI) nilotinib (Novartis Pharma Stein AG); human recombinant H-IFN-γ (PeproTech, US), with a final concentration of 150 U/ml; c-Abl activator DPH (MedChemExpress, US), with a final concentration of 10 mM.

Plasmid Preparation:

Article Title: Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma Cells
Article Snippet: .. Main reagents The main reagents were Opti-MEM (Thermo Fisher Scientific Inc., USA); polymerase chain reaction (PCR) mix (Tiangen Biotech (Beijing) CO., LTD.); real-time fluorescence quantitative PCR (RT-PCR) mixture and iTaq™ SYBR Green Supermix (Bio-Rad); β5i (LMP7) antibody (Santa Cruz Biotechnology); horseradish peroxidase (HRP) -labeled β-actin mouse monoclonal antibody (Millipore Sigma Canada Co.); HRP-labeled rabbit anti-goat antibody (Amersham Pharmacia Biotech); complementary DNA (cDNA) RT-PCR kit (Promega GoScript™ Reverse Transcription System); γ-ray irradiation source: 60 Co (Beijing Institute of Biotechnology, Beijing 100850, China); plasmid vectors pLKO.1-TRC (Addgene plasmid #10878; http://n2t.net/addgene: 10878; RRID: Addgene_10878), psPAX2, and pMD2. .. G (Invitrogen); DNA restriction endonucleases AgeI (NEB, #R0552S), EcoRI (NEB, #R0101S), and NcoI (NEB, #R3193), along with Buffer 2 (NEB, #B7002S) and T4 DNA Ligase (NEB, #M0202S); FuGENE HD transfection reagent (Roche); molecular weight standard: DNA Marker (DL2000, DL15000) (Tiangen Biotech (Beijing)); low-molecular-weight protein standard (PageRuler Prestained protein ladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction and purification kit (Tiangen Biotech, Beijing); the exogenous tyrosine kinase inhibitor (TKI) nilotinib (Novartis Pharma Stein AG); human recombinant H-IFN-γ (PeproTech, US), with a final concentration of 150 U/ml; c-Abl activator DPH (MedChemExpress, US), with a final concentration of 10 mM.

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    tiangen biotech co pcr master mix
    BstUI <t>PCR‐RFLP</t> analysis of the PLIN1 biallelic polymorphism. M, <t>DNA</t> ladder; Lane1, AA; Lane2, AG; Lane3, GG.
    Pcr Master Mix, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/tiangen biotech co
    Average 94 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    tiangen biotech co quantitative rt pcr qrt pcr fastquant rt super mix
    Validation of the differentially expressed circRNAs. To confirm the findings from the microarray analysis, the relative expression of circRNAs was measured by <t>qRT-PCR.</t> Five out of the 7 significantly differentially expressed circRNAs were measured. Note that some circRNAs have not yet been named yet, and are tentatively named here using the “chromosome: start_position” format. * p
    Quantitative Rt Pcr Qrt Pcr Fastquant Rt Super Mix, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative rt pcr qrt pcr fastquant rt super mix/product/tiangen biotech co
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative rt pcr qrt pcr fastquant rt super mix - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    BstUI PCR‐RFLP analysis of the PLIN1 biallelic polymorphism. M, DNA ladder; Lane1, AA; Lane2, AG; Lane3, GG.

    Journal: Journal of Clinical Laboratory Analysis

    Article Title: An Improved PCR‐RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene

    doi: 10.1002/jcla.21968

    Figure Lengend Snippet: BstUI PCR‐RFLP analysis of the PLIN1 biallelic polymorphism. M, DNA ladder; Lane1, AA; Lane2, AG; Lane3, GG.

    Article Snippet: PCR was performed in a total volume of 25 μL containing 100 ng of genomic DNA, 1× PCR master Mix (Tiangen), and 5 pmol of each primer.

    Techniques: Polymerase Chain Reaction

    Examples of DNA sequencing of PCR product of PLIN1 gene.

    Journal: Journal of Clinical Laboratory Analysis

    Article Title: An Improved PCR‐RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene

    doi: 10.1002/jcla.21968

    Figure Lengend Snippet: Examples of DNA sequencing of PCR product of PLIN1 gene.

    Article Snippet: PCR was performed in a total volume of 25 μL containing 100 ng of genomic DNA, 1× PCR master Mix (Tiangen), and 5 pmol of each primer.

    Techniques: DNA Sequencing, Polymerase Chain Reaction

    Effect of daphnetin and 5-aza-dc on expression of DR3, PDCD5, FasL, p53 (A) and DNMT1, DNMT3a, DNMT3b (B) in CIA rat synovial cells. Four experimental groups consisting of untreated cell control, or treated with 5-aza-dc at 20 μM, daphnetin at 40 μg/mL, or combination of 5-aza-dc (20 μM) and daphnetin (40 μg/mL). Cells were cultured, treated and harvested as described in Matreials and Methods. Total RNA was extracted and cDNA was synthesized. After reverse transcription, cDNA was used for real time-PCR. Relative quantification of gene expression was performed by the 2-∆∆Ct method. The results show the mean ± S.D. of six independent experiments. ▲P

    Journal: Journal of Translational Medicine

    Article Title: Therapeutic effect of daphnetin on the autoimmune arthritis through demethylation of proapoptotic genes in synovial cells

    doi: 10.1186/s12967-014-0287-x

    Figure Lengend Snippet: Effect of daphnetin and 5-aza-dc on expression of DR3, PDCD5, FasL, p53 (A) and DNMT1, DNMT3a, DNMT3b (B) in CIA rat synovial cells. Four experimental groups consisting of untreated cell control, or treated with 5-aza-dc at 20 μM, daphnetin at 40 μg/mL, or combination of 5-aza-dc (20 μM) and daphnetin (40 μg/mL). Cells were cultured, treated and harvested as described in Matreials and Methods. Total RNA was extracted and cDNA was synthesized. After reverse transcription, cDNA was used for real time-PCR. Relative quantification of gene expression was performed by the 2-∆∆Ct method. The results show the mean ± S.D. of six independent experiments. ▲P

    Article Snippet: Real-time PCR was performed in the ABI 7500 sequence detection system (Applied Biosystems) with a reaction mixture that consisted of SYBR Green 2 × PCR Master Mix (Tiangen biotech co., LTD., Beijing, China), cDNA template, forward primer and reverse primer.

    Techniques: Expressing, Cell Culture, Synthesized, Real-time Polymerase Chain Reaction

    Construction and assay of pEGFP-C1/HHEX. (A) HHEX cDNA electrophoresis products of PCR (1: DNA marker III, 2: HHEX cDNA, 3: negative control); (B) pGM-T/HHEX digestion products (1: DNA marker III, 2: pGM-T/HHEXdigestion products); (C) pEGFP-C1/HHEX digestion products (1: 1Kb DNA marker; 2: pEGFP-C1 digestion products; 3: pEGFP-C1/HHEX digestion products); (D) Normal control; (E) pEGFP-C1; (F) pEGFP-C1/HHEX.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: HHEX: A Crosstalker between HCMV Infection and Proliferation of VSMCs

    doi: 10.3389/fcimb.2016.00169

    Figure Lengend Snippet: Construction and assay of pEGFP-C1/HHEX. (A) HHEX cDNA electrophoresis products of PCR (1: DNA marker III, 2: HHEX cDNA, 3: negative control); (B) pGM-T/HHEX digestion products (1: DNA marker III, 2: pGM-T/HHEXdigestion products); (C) pEGFP-C1/HHEX digestion products (1: 1Kb DNA marker; 2: pEGFP-C1 digestion products; 3: pEGFP-C1/HHEX digestion products); (D) Normal control; (E) pEGFP-C1; (F) pEGFP-C1/HHEX.

    Article Snippet: Plasmid extraction kit and DNA gel extraction kit were supplied by OMEGA, USA. pGM-T vector kit and PCR master mix were from Tiangen (Beijing) Co., Ltd. TRIzol and Lipofectamine LTX and PLUS reagents were from Life Technologies, USA. cDNA first strand synthesis kit was from Toyobo (Shanghai) biological Technology Co. CCK 8 cell proliferation test kit was supplied by Dongren chemical technology (Shanghai) Co., LTD. Hoechst33342/PI double dye cell apoptosis detection kit was from Beibo (Shanghai) biological technology Co., LTD.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Marker, Negative Control

    Validation of the differentially expressed circRNAs. To confirm the findings from the microarray analysis, the relative expression of circRNAs was measured by qRT-PCR. Five out of the 7 significantly differentially expressed circRNAs were measured. Note that some circRNAs have not yet been named yet, and are tentatively named here using the “chromosome: start_position” format. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Molecular and Bioinformatics Analyses Identify 7 Circular RNAs Involved in Regulation of Oncogenic Transformation and Cell Proliferation in Human Bladder Cancer

    doi: 10.12659/MSM.908837

    Figure Lengend Snippet: Validation of the differentially expressed circRNAs. To confirm the findings from the microarray analysis, the relative expression of circRNAs was measured by qRT-PCR. Five out of the 7 significantly differentially expressed circRNAs were measured. Note that some circRNAs have not yet been named yet, and are tentatively named here using the “chromosome: start_position” format. * p

    Article Snippet: Quantitative RT-PCR (qRT-PCR) FastQuant RT Super Mix (Tiangen, Beijing, China) was used to synthesize cDNA templates.

    Techniques: Microarray, Expressing, Quantitative RT-PCR