polymerase chain reaction pcr mixture  (Thermo Fisher)


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    Structured Review

    Thermo Fisher polymerase chain reaction pcr mixture
    The genotype characteristic of SNP rs231775 in CTLA-4 gene in AA and HIs. a Distribution of the genotype of SNP rs231775 in AA patients ( n = 67); b distribution of the genotype of SNP rs231775 in HIs ( n = 84); c agarose gel electrophoresis results for SNP rs231775 in CTLA-4 gene, lane 1 is the AG genotype, lane 2 is the GG genotype, lane 3 is a 50 bp <t>DNA</t> ladder, lane 4 is the AA genotype, and lane 5 is a <t>PCR</t> product without TseI digestion; d sequencing results of AA genotype; e sequencing results of AG genotype; f sequencing results of GG genotype; the red arrow indicates the single-nucleotide polymorphism sites
    Polymerase Chain Reaction Pcr Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polymerase chain reaction pcr mixture - by Bioz Stars, 2021-01
    98/100 stars

    Images

    1) Product Images from "Molecular alterations in the TCR signaling pathway in patients with aplastic anemia"

    Article Title: Molecular alterations in the TCR signaling pathway in patients with aplastic anemia

    Journal: Journal of Hematology & Oncology

    doi: 10.1186/s13045-016-0261-6

    The genotype characteristic of SNP rs231775 in CTLA-4 gene in AA and HIs. a Distribution of the genotype of SNP rs231775 in AA patients ( n = 67); b distribution of the genotype of SNP rs231775 in HIs ( n = 84); c agarose gel electrophoresis results for SNP rs231775 in CTLA-4 gene, lane 1 is the AG genotype, lane 2 is the GG genotype, lane 3 is a 50 bp DNA ladder, lane 4 is the AA genotype, and lane 5 is a PCR product without TseI digestion; d sequencing results of AA genotype; e sequencing results of AG genotype; f sequencing results of GG genotype; the red arrow indicates the single-nucleotide polymorphism sites
    Figure Legend Snippet: The genotype characteristic of SNP rs231775 in CTLA-4 gene in AA and HIs. a Distribution of the genotype of SNP rs231775 in AA patients ( n = 67); b distribution of the genotype of SNP rs231775 in HIs ( n = 84); c agarose gel electrophoresis results for SNP rs231775 in CTLA-4 gene, lane 1 is the AG genotype, lane 2 is the GG genotype, lane 3 is a 50 bp DNA ladder, lane 4 is the AA genotype, and lane 5 is a PCR product without TseI digestion; d sequencing results of AA genotype; e sequencing results of AG genotype; f sequencing results of GG genotype; the red arrow indicates the single-nucleotide polymorphism sites

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Sequencing

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Using an in-vitro biofilm model to assess the virulence potential of Bacterial Vaginosis or non-Bacterial Vaginosis Gardnerella vaginalis isolates
    Article Snippet: .. Genomic DNA was extracted as described before and the thermocycling program (Mini-MJ, Bio-Rad, Hercules, CA, USA) was performed using the DreamTaq PCR Master Mix 2x (Finnzymes, Espoo, Finland) and consisted on the following steps: 94 °C for 2 minutes followed by 40 cycles of 94 °C for 30 seconds, 58 °C for 30 seconds, 72 °C for 60 seconds and finally 72 °C for 5 minutes. ..

    Article Title: Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts
    Article Snippet: .. For analysis of alternative splicing, Meioc +/+ and Meioc −/− 20-d.p.p. testis mRNAs were reverse-transcripted and full-length cDNAs were amplified using PCR with DreamTaq PCR Master Mix (2 × ; Life Technologies). .. Full-length PCR amplicons were purified with the QIAquick PCR Purification Kit or QIAquick Gel Extraction Kit (QIAGEN) and were submitted to SUPREMErunTM sequencing (GATC).

    Article Title: Seroprevalence and current infections of canine vector-borne diseases in Nicaragua
    Article Snippet: .. In the first round, the 25 μl reaction volume contained 12.5 μl DreamTaq® PCR Mastermix, 0.5 μl of ECC (0.4 μM), 0.5 μl of ECB (10 μM), 9.5 μl deionized water and 2 μl template DNA, while in the second round, 0.5 μl each of primers ECAN5 (0.4 μM) and HE3 (10 μM) as well as 2.5 μl of the PCR-product of the first round were used, and the amount of water was adjusted to 9 μl. ..

    Article Title: Phenotype-Independent Isolation of Interspecies Saccharomyces Hybrids by Dual-Dye Fluorescent Staining and Fluorescence-Activated Cell Sorting
    Article Snippet: .. Identification of Interspecies Hybrids by PCR The presence of genetic material from S. cerevisiae and from S. eubayanus and the mating type of potential hybrids was verified by PCR using DreamTaq PCR Mastermix (Life Technologies), as described previously ( ). .. To ensure that single cells isolates were tested, sorted dual-stained cells were grown in YPD and a second FACS step was used to sort a single cell from each culture.

    Article Title: The life-cycle of the avian haemosporidian parasite Haemoproteus majoris, with emphasis on the exoerythrocytic and sporogonic development
    Article Snippet: .. The total volume of the PCR mix was 25 µl and consisted of 12.5 µl of Dreamtaq Master Mix (Thermo Fisher Scientific), 8.5 µl of nuclease-free water, 1 µl of each primer and 2 µl of template DNA. .. The primer pair HQF/HQR, which amplifies a short (194 bp) fragment of the mitochondrial cytb gene, was used for the PCR according to the conditions described by Ciloglu et al. [ ].

    Article Title: Interleukin 10 (− 1082 G/A) and (− 819 C/T) gene polymorphisms in Egyptian women with polycystic ovary syndrome (PCOS)
    Article Snippet: .. The PCR mixtures consisted of DreamTaq Green PCR Master Mix (2 ×) (Fermentas), 10 pmol of each allele-specific primer, 10 pmol of reverse primer, 3.5 pmol of each control primer and 100 ng of DNA. ..

    Article Title: Detection of a Planktothrix agardhii Bloom in Portuguese Marine Coastal Waters
    Article Snippet: .. The amplifications were performed in a reaction mixture containing DreamTaq PCR Master Mix (ThermoFisher Scientific® ) with 1 U of Taq DNA polymerase using a T100™ Thermal Cycler (BioRad) programmed with a PCR cycle consisting of an initial denaturation step at 94 °C for 3 min, followed by 40 cycles of 20 s at 94 °C, 30 s at 55 °C and 20 s at 72 °C and a final extension step of 5 min at 72 °C. .. The amplified fragments were visualized under UV light after electrophoretic analysis performed in 1% w/v agarose gel with GreenSafe Premium™ DNA staining (NZYTech® ), at 80 V in 0.5× Tris-borate EDTA (TBE) buffer for 45 min.

    Article Title: Quantitative Monitoring of Microbial Species during Bioleaching of a Copper Concentrate
    Article Snippet: .. T-RFLP Monitoring Amplification of a 900 bp fragment of the 16S rRNA gene for T-RFLP analysis from DNA extracts was achieved using DreamTaq PCR Master Mix (Thermo Fisher) and a Cy5-labeled 8F forward primer ( ) and 907R ( ) as described previously ( ). .. Up to 4 μL of PCR product were digested in a 10 μL reaction with 1 U of the restriction endonuclease HaeIII (Thermo Scientific) and 1 μL appropriate buffer.

    Amplification:

    Article Title: Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts
    Article Snippet: .. For analysis of alternative splicing, Meioc +/+ and Meioc −/− 20-d.p.p. testis mRNAs were reverse-transcripted and full-length cDNAs were amplified using PCR with DreamTaq PCR Master Mix (2 × ; Life Technologies). .. Full-length PCR amplicons were purified with the QIAquick PCR Purification Kit or QIAquick Gel Extraction Kit (QIAGEN) and were submitted to SUPREMErunTM sequencing (GATC).

    Article Title: Quantitative Monitoring of Microbial Species during Bioleaching of a Copper Concentrate
    Article Snippet: .. T-RFLP Monitoring Amplification of a 900 bp fragment of the 16S rRNA gene for T-RFLP analysis from DNA extracts was achieved using DreamTaq PCR Master Mix (Thermo Fisher) and a Cy5-labeled 8F forward primer ( ) and 907R ( ) as described previously ( ). .. Up to 4 μL of PCR product were digested in a 10 μL reaction with 1 U of the restriction endonuclease HaeIII (Thermo Scientific) and 1 μL appropriate buffer.

    Terminal Restriction Fragment Length Polymorphism:

    Article Title: Quantitative Monitoring of Microbial Species during Bioleaching of a Copper Concentrate
    Article Snippet: .. T-RFLP Monitoring Amplification of a 900 bp fragment of the 16S rRNA gene for T-RFLP analysis from DNA extracts was achieved using DreamTaq PCR Master Mix (Thermo Fisher) and a Cy5-labeled 8F forward primer ( ) and 907R ( ) as described previously ( ). .. Up to 4 μL of PCR product were digested in a 10 μL reaction with 1 U of the restriction endonuclease HaeIII (Thermo Scientific) and 1 μL appropriate buffer.

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    Thermo Fisher genes has1 3
    Factor analysis for correlation of gene expression levels. <t>HAS1-3</t> , HYAL1-2 , CEMIP , CD44 , VCAN , and TSG6 in non-failing left wall and septum (n = 9) ( a ) and HCM myectomies (n = 5) ( b ). ( a ) In left wall and septum there are two clusters where HAS1 , CEMIP , CD44 , VCAN , and TSG6 form one cluster, and HAS2 , HAS3, and HYAL2 form another. ( b ) In basal septal myectomies from HCM patients the expression levels of CEMIP , CD44, and VCAN formed a new correlation cluster with HAS3 . HAS2 , HYAL1 , and TSG6 formed another cluster. The levels of HAS1 and HYAL2 no longer correlated with any of the other genes investigated. Factor analysis was performed with the principal components method to analyze the correlation matrix and two factors were extracted.
    Genes Has1 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher qrt pcr reaction mixture
    Effect of transient overexpression of G(G)PPS and GES in C. roseus leaves. mRNA expression (A,B) and metabolite (C,D) analyses in C. roseus leaves infiltrated with A. tumefaciens carrying pBI121 empty vector (black bar), pBI121:: G(G)PPS and co-infiltrated with pBI121:: G(G)PPS +pBI121:: GES (gray bar) constructs. Transcripts were analyzed by <t>qRT-PCR</t> with CrN227 as an endogenous reference gene. Expression levels were normalized to CrN227 and are represented as expression relative to the pBI121 controls that was set to 1. Relative amounts of secologanin, vindoline, and catharanthine in C. roseus . In all cases, first pair of infiltrated leaves were used for alkaloid extraction and quantified by HPLC. The levels of quantified metabolites are expressed in % relative to pBI121 vector infiltrated leaves. The bars represent mean ± standard error (SE) of three independent experiments. Significant differences at P
    Qrt Pcr Reaction Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ln229 glioma cells
    GSK343 treatment leads to downregulation of H3K27me3 and increases expression of E-cadherin, p21 and PTEN (A) Expression of H3K27me3 in U87 and <t>LN229</t> cells treated with 5 μM GSK343 over time was determined by western blot analysis. GADPH was used as a loading control. (B) U87 and LN229 cells were treated with 5 μM GSK343 or 0.1% DMSO for 48 h and the levels of EZH2, EED, SUZ12, and GAPDH were examined by western blot analysis. (C) Co-immunoprecipitation analyses of EZH2-H3 complex formation in U87 and LN229 cells after treatment with 5 μM GSK343 for 12 h or 24 h. (D) Western blot analysis of E-cadherin, p21, and PTEN expression in cells treated with 5 μM, 7.5μM GSK343 and 0.1% DMSO for 48 h.
    Ln229 Glioma Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher l4 l5 drgs
    Expression changes of p-AXL- and AXL-positive neurons in the DRG in CCD-induced neuropathic pain model. (a and b) The percentage of p-AXL-positive (+) neurons increased in compressed <t>L4/L5</t> DRGs of CCD rats. The value on each histogram shows the ratio of p-AXL (+) neurons to total counted neurons in DRG slices. N = 5 rats/group. ** P
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    Factor analysis for correlation of gene expression levels. HAS1-3 , HYAL1-2 , CEMIP , CD44 , VCAN , and TSG6 in non-failing left wall and septum (n = 9) ( a ) and HCM myectomies (n = 5) ( b ). ( a ) In left wall and septum there are two clusters where HAS1 , CEMIP , CD44 , VCAN , and TSG6 form one cluster, and HAS2 , HAS3, and HYAL2 form another. ( b ) In basal septal myectomies from HCM patients the expression levels of CEMIP , CD44, and VCAN formed a new correlation cluster with HAS3 . HAS2 , HYAL1 , and TSG6 formed another cluster. The levels of HAS1 and HYAL2 no longer correlated with any of the other genes investigated. Factor analysis was performed with the principal components method to analyze the correlation matrix and two factors were extracted.

    Journal: Cells

    Article Title: Low Molecular Mass Myocardial Hyaluronan in Human Hypertrophic Cardiomyopathy

    doi: 10.3390/cells8020097

    Figure Lengend Snippet: Factor analysis for correlation of gene expression levels. HAS1-3 , HYAL1-2 , CEMIP , CD44 , VCAN , and TSG6 in non-failing left wall and septum (n = 9) ( a ) and HCM myectomies (n = 5) ( b ). ( a ) In left wall and septum there are two clusters where HAS1 , CEMIP , CD44 , VCAN , and TSG6 form one cluster, and HAS2 , HAS3, and HYAL2 form another. ( b ) In basal septal myectomies from HCM patients the expression levels of CEMIP , CD44, and VCAN formed a new correlation cluster with HAS3 . HAS2 , HYAL1 , and TSG6 formed another cluster. The levels of HAS1 and HYAL2 no longer correlated with any of the other genes investigated. Factor analysis was performed with the principal components method to analyze the correlation matrix and two factors were extracted.

    Article Snippet: The real-time quantitative PCR was performed on a 7900 HT Fast Real-Time PCR system (Thermo Fisher Scientific, MA, USA) using 1 µg cDNA, TaqMan® Gene Expression Assays, and 1 µL Gene Assay Mix for the genes HAS1-3 , HYAL 1 and 2 , CEMIP , CD44 , VCAN , and TSG6 (Thermo Fisher Scientific, MA, USA).

    Techniques: Expressing

    Effect of transient overexpression of G(G)PPS and GES in C. roseus leaves. mRNA expression (A,B) and metabolite (C,D) analyses in C. roseus leaves infiltrated with A. tumefaciens carrying pBI121 empty vector (black bar), pBI121:: G(G)PPS and co-infiltrated with pBI121:: G(G)PPS +pBI121:: GES (gray bar) constructs. Transcripts were analyzed by qRT-PCR with CrN227 as an endogenous reference gene. Expression levels were normalized to CrN227 and are represented as expression relative to the pBI121 controls that was set to 1. Relative amounts of secologanin, vindoline, and catharanthine in C. roseus . In all cases, first pair of infiltrated leaves were used for alkaloid extraction and quantified by HPLC. The levels of quantified metabolites are expressed in % relative to pBI121 vector infiltrated leaves. The bars represent mean ± standard error (SE) of three independent experiments. Significant differences at P

    Journal: Frontiers in Plant Science

    Article Title: Terpene Moiety Enhancement by Overexpression of Geranyl(geranyl) Diphosphate Synthase and Geraniol Synthase Elevates Monomeric and Dimeric Monoterpene Indole Alkaloids in Transgenic Catharanthus roseus

    doi: 10.3389/fpls.2018.00942

    Figure Lengend Snippet: Effect of transient overexpression of G(G)PPS and GES in C. roseus leaves. mRNA expression (A,B) and metabolite (C,D) analyses in C. roseus leaves infiltrated with A. tumefaciens carrying pBI121 empty vector (black bar), pBI121:: G(G)PPS and co-infiltrated with pBI121:: G(G)PPS +pBI121:: GES (gray bar) constructs. Transcripts were analyzed by qRT-PCR with CrN227 as an endogenous reference gene. Expression levels were normalized to CrN227 and are represented as expression relative to the pBI121 controls that was set to 1. Relative amounts of secologanin, vindoline, and catharanthine in C. roseus . In all cases, first pair of infiltrated leaves were used for alkaloid extraction and quantified by HPLC. The levels of quantified metabolites are expressed in % relative to pBI121 vector infiltrated leaves. The bars represent mean ± standard error (SE) of three independent experiments. Significant differences at P

    Article Snippet: Total RNA (2 μg) was used for first strand cDNA synthesis with random hexamer primers using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, United States) as per manufacturer’s instructions. qRT-PCR was performed with a linear range of cDNA using StepOne Real-Time PCR System (Applied Biosystems, United States) and expression of transcripts were normalized using N227 that was previously reported as an appropriate endogenous gene in C. roseus ( ). qRT-PCR reaction mixture of 5 μl contained 2.5 μl of 2X Maxima SYBR Green/ROX qPCR master mix (Thermo Scientific, United States), 1:3 diluted cDNA and 2 μM gene-specific primers (Supplementary Table ).

    Techniques: Over Expression, Expressing, Plasmid Preparation, Construct, Quantitative RT-PCR, High Performance Liquid Chromatography

    Analysis of gene expression in transgenic G(G)PPS and GES C. roseus plants. qRT-PCR analysis of G(G)PPS (gray bar) (A) and G(G)PPS (gray bar)/ GES (black bar) (B) in transgenic C. roseus plants. Expression levels of genes were normalized to the endogenous reference gene CrN227 and are represented relative to the wild type (WT) controls, which was set to 1. Error bars represent mean ± standard error (SE) of three independent experiments. Significant differences at P

    Journal: Frontiers in Plant Science

    Article Title: Terpene Moiety Enhancement by Overexpression of Geranyl(geranyl) Diphosphate Synthase and Geraniol Synthase Elevates Monomeric and Dimeric Monoterpene Indole Alkaloids in Transgenic Catharanthus roseus

    doi: 10.3389/fpls.2018.00942

    Figure Lengend Snippet: Analysis of gene expression in transgenic G(G)PPS and GES C. roseus plants. qRT-PCR analysis of G(G)PPS (gray bar) (A) and G(G)PPS (gray bar)/ GES (black bar) (B) in transgenic C. roseus plants. Expression levels of genes were normalized to the endogenous reference gene CrN227 and are represented relative to the wild type (WT) controls, which was set to 1. Error bars represent mean ± standard error (SE) of three independent experiments. Significant differences at P

    Article Snippet: Total RNA (2 μg) was used for first strand cDNA synthesis with random hexamer primers using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, United States) as per manufacturer’s instructions. qRT-PCR was performed with a linear range of cDNA using StepOne Real-Time PCR System (Applied Biosystems, United States) and expression of transcripts were normalized using N227 that was previously reported as an appropriate endogenous gene in C. roseus ( ). qRT-PCR reaction mixture of 5 μl contained 2.5 μl of 2X Maxima SYBR Green/ROX qPCR master mix (Thermo Scientific, United States), 1:3 diluted cDNA and 2 μM gene-specific primers (Supplementary Table ).

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR

    PRX1 expression in transgenic C. roseus plants. Analysis of PRX1 expression by qRT-PCR in G(G)PPS (A) and G(G)PPS + GES (B) overexpressing transgenic C. roseus plants. Expression levels of PRX1 were normalized to the endogenous reference gene CrN227 and are represented relative to the WT controls, which was set to 1. Error bars represent mean ± standard error (SE) of three independent experiments. Significant differences at P

    Journal: Frontiers in Plant Science

    Article Title: Terpene Moiety Enhancement by Overexpression of Geranyl(geranyl) Diphosphate Synthase and Geraniol Synthase Elevates Monomeric and Dimeric Monoterpene Indole Alkaloids in Transgenic Catharanthus roseus

    doi: 10.3389/fpls.2018.00942

    Figure Lengend Snippet: PRX1 expression in transgenic C. roseus plants. Analysis of PRX1 expression by qRT-PCR in G(G)PPS (A) and G(G)PPS + GES (B) overexpressing transgenic C. roseus plants. Expression levels of PRX1 were normalized to the endogenous reference gene CrN227 and are represented relative to the WT controls, which was set to 1. Error bars represent mean ± standard error (SE) of three independent experiments. Significant differences at P

    Article Snippet: Total RNA (2 μg) was used for first strand cDNA synthesis with random hexamer primers using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, United States) as per manufacturer’s instructions. qRT-PCR was performed with a linear range of cDNA using StepOne Real-Time PCR System (Applied Biosystems, United States) and expression of transcripts were normalized using N227 that was previously reported as an appropriate endogenous gene in C. roseus ( ). qRT-PCR reaction mixture of 5 μl contained 2.5 μl of 2X Maxima SYBR Green/ROX qPCR master mix (Thermo Scientific, United States), 1:3 diluted cDNA and 2 μM gene-specific primers (Supplementary Table ).

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR

    GSK343 treatment leads to downregulation of H3K27me3 and increases expression of E-cadherin, p21 and PTEN (A) Expression of H3K27me3 in U87 and LN229 cells treated with 5 μM GSK343 over time was determined by western blot analysis. GADPH was used as a loading control. (B) U87 and LN229 cells were treated with 5 μM GSK343 or 0.1% DMSO for 48 h and the levels of EZH2, EED, SUZ12, and GAPDH were examined by western blot analysis. (C) Co-immunoprecipitation analyses of EZH2-H3 complex formation in U87 and LN229 cells after treatment with 5 μM GSK343 for 12 h or 24 h. (D) Western blot analysis of E-cadherin, p21, and PTEN expression in cells treated with 5 μM, 7.5μM GSK343 and 0.1% DMSO for 48 h.

    Journal: Oncotarget

    Article Title: The EZH2 inhibitor GSK343 suppresses cancer stem-like phenotypes and reverses mesenchymal transition in glioma cells

    doi: 10.18632/oncotarget.21311

    Figure Lengend Snippet: GSK343 treatment leads to downregulation of H3K27me3 and increases expression of E-cadherin, p21 and PTEN (A) Expression of H3K27me3 in U87 and LN229 cells treated with 5 μM GSK343 over time was determined by western blot analysis. GADPH was used as a loading control. (B) U87 and LN229 cells were treated with 5 μM GSK343 or 0.1% DMSO for 48 h and the levels of EZH2, EED, SUZ12, and GAPDH were examined by western blot analysis. (C) Co-immunoprecipitation analyses of EZH2-H3 complex formation in U87 and LN229 cells after treatment with 5 μM GSK343 for 12 h or 24 h. (D) Western blot analysis of E-cadherin, p21, and PTEN expression in cells treated with 5 μM, 7.5μM GSK343 and 0.1% DMSO for 48 h.

    Article Snippet: 5-ethynyl-29-deoxyuridine (EdU) proliferation assay To assess the proliferative activity of U87 and LN229 glioma cells, Click-iT EdU Alexa Fluor 594 Imaging Kit (Thermo Fisher Scientific, Massachusetts, USA; Cat. No. C10339) was used according to the manufacturer's protocol.

    Techniques: Expressing, Western Blot, Immunoprecipitation

    GSK343 suppresses mesenchymal transition in U87 and LN229 cells (A, B) Protein and mRNA levels of N-cadherin, Vimentin, MMP2, MMP9, Snail, Slug, and GAPDH in U87 and LN229 cells treated with 5 μM GSK343 or 0.1% DMSO for 48 h were examined by western blot analysis and RT-PCR ( * p

    Journal: Oncotarget

    Article Title: The EZH2 inhibitor GSK343 suppresses cancer stem-like phenotypes and reverses mesenchymal transition in glioma cells

    doi: 10.18632/oncotarget.21311

    Figure Lengend Snippet: GSK343 suppresses mesenchymal transition in U87 and LN229 cells (A, B) Protein and mRNA levels of N-cadherin, Vimentin, MMP2, MMP9, Snail, Slug, and GAPDH in U87 and LN229 cells treated with 5 μM GSK343 or 0.1% DMSO for 48 h were examined by western blot analysis and RT-PCR ( * p

    Article Snippet: 5-ethynyl-29-deoxyuridine (EdU) proliferation assay To assess the proliferative activity of U87 and LN229 glioma cells, Click-iT EdU Alexa Fluor 594 Imaging Kit (Thermo Fisher Scientific, Massachusetts, USA; Cat. No. C10339) was used according to the manufacturer's protocol.

    Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction

    GSK343 suppresses cell proliferation, migration and invasion and induces G 0 /G 1 arrest in glioma cells (A) Dose-dependent effects of GSK343 (5 μM, 7.5 μM, and 10 μM) on cell proliferation over time in U87 and LN229 cells. Cell growth was measured by the cell counting kit-8 ( * p

    Journal: Oncotarget

    Article Title: The EZH2 inhibitor GSK343 suppresses cancer stem-like phenotypes and reverses mesenchymal transition in glioma cells

    doi: 10.18632/oncotarget.21311

    Figure Lengend Snippet: GSK343 suppresses cell proliferation, migration and invasion and induces G 0 /G 1 arrest in glioma cells (A) Dose-dependent effects of GSK343 (5 μM, 7.5 μM, and 10 μM) on cell proliferation over time in U87 and LN229 cells. Cell growth was measured by the cell counting kit-8 ( * p

    Article Snippet: 5-ethynyl-29-deoxyuridine (EdU) proliferation assay To assess the proliferative activity of U87 and LN229 glioma cells, Click-iT EdU Alexa Fluor 594 Imaging Kit (Thermo Fisher Scientific, Massachusetts, USA; Cat. No. C10339) was used according to the manufacturer's protocol.

    Techniques: Migration, Cell Counting

    Expression changes of p-AXL- and AXL-positive neurons in the DRG in CCD-induced neuropathic pain model. (a and b) The percentage of p-AXL-positive (+) neurons increased in compressed L4/L5 DRGs of CCD rats. The value on each histogram shows the ratio of p-AXL (+) neurons to total counted neurons in DRG slices. N = 5 rats/group. ** P

    Journal: Molecular Pain

    Article Title: AXL signaling in primary sensory neurons contributes to chronic compression of dorsal root ganglion-induced neuropathic pain in rats

    doi: 10.1177/1744806919900814

    Figure Lengend Snippet: Expression changes of p-AXL- and AXL-positive neurons in the DRG in CCD-induced neuropathic pain model. (a and b) The percentage of p-AXL-positive (+) neurons increased in compressed L4/L5 DRGs of CCD rats. The value on each histogram shows the ratio of p-AXL (+) neurons to total counted neurons in DRG slices. N = 5 rats/group. ** P

    Article Snippet: RNA extraction, reverse transcription, and quantitative real-time polymerase chain reaction Rats were first anesthetized and then killed by decapitation, and both L4/L5 DRGs and L4/L5 spinal cord segments were harvested and placed into RNAlater stabilization solution (Thermo Scientific).

    Techniques: Expressing

    Expression of p-AXL and AXL in DRG. (a) The expression of p-AXL, AXL, and negative control without primary antibodies in naive L4/L5 DRGs. Histogram shows the distribution of p-AXL (b) and AXL (c) somata in naive L4/L5 DRGs. N = 3 rats, 6 to 8 slices per rat. (d) The colocalization of p-AXL with IB4, CGRP, NF200, or GS. (e) The colocalization of AXL with IB4, CGRP, NF200, or GS. Arrows: examples of double-labeled neurons. Scale bars: 100 μm (a); 25 μm (c and d). IB4: isolectin B4; CGRP: calcitonin gene-related peptide; NF200: neurofilament-200; p-AXL: phosphorylated AXL; GS: glutamine synthetase.

    Journal: Molecular Pain

    Article Title: AXL signaling in primary sensory neurons contributes to chronic compression of dorsal root ganglion-induced neuropathic pain in rats

    doi: 10.1177/1744806919900814

    Figure Lengend Snippet: Expression of p-AXL and AXL in DRG. (a) The expression of p-AXL, AXL, and negative control without primary antibodies in naive L4/L5 DRGs. Histogram shows the distribution of p-AXL (b) and AXL (c) somata in naive L4/L5 DRGs. N = 3 rats, 6 to 8 slices per rat. (d) The colocalization of p-AXL with IB4, CGRP, NF200, or GS. (e) The colocalization of AXL with IB4, CGRP, NF200, or GS. Arrows: examples of double-labeled neurons. Scale bars: 100 μm (a); 25 μm (c and d). IB4: isolectin B4; CGRP: calcitonin gene-related peptide; NF200: neurofilament-200; p-AXL: phosphorylated AXL; GS: glutamine synthetase.

    Article Snippet: RNA extraction, reverse transcription, and quantitative real-time polymerase chain reaction Rats were first anesthetized and then killed by decapitation, and both L4/L5 DRGs and L4/L5 spinal cord segments were harvested and placed into RNAlater stabilization solution (Thermo Scientific).

    Techniques: Expressing, Negative Control, Labeling

    Mediation by the intracellular signaling molecule, mTOR, of nociceptive effects of AXL. (a) The protein amount of p-mTOR and total mTOR increased in the ipsilateral L4/L5 DRGs on days 7 and 14 after CCD surgery. N = 3 rats/time point. One-way ANOVA (expression vs. time points) followed by post hoc Tukey test, * P

    Journal: Molecular Pain

    Article Title: AXL signaling in primary sensory neurons contributes to chronic compression of dorsal root ganglion-induced neuropathic pain in rats

    doi: 10.1177/1744806919900814

    Figure Lengend Snippet: Mediation by the intracellular signaling molecule, mTOR, of nociceptive effects of AXL. (a) The protein amount of p-mTOR and total mTOR increased in the ipsilateral L4/L5 DRGs on days 7 and 14 after CCD surgery. N = 3 rats/time point. One-way ANOVA (expression vs. time points) followed by post hoc Tukey test, * P

    Article Snippet: RNA extraction, reverse transcription, and quantitative real-time polymerase chain reaction Rats were first anesthetized and then killed by decapitation, and both L4/L5 DRGs and L4/L5 spinal cord segments were harvested and placed into RNAlater stabilization solution (Thermo Scientific).

    Techniques: Expressing