polymerase chain reaction pcr mixture (TaKaRa)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

TaKaRa polymerase chain reaction pcr mixture
Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

Polymerase Chain Reaction Pcr Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr mixture/product/TaKaRa
Average 91 stars, based on 31 article reviews
Price from $9.99 to $1999.99

polymerase chain reaction pcr mixture - by Bioz Stars, 2020-08

91/100 stars

Related Products / Commonly Used Together

mgcl2
dntps
dntp
taq dna polymerase
taq polymerase
pcr buffer
cdna
template dna
hotstar taq dna polymerase
dntp mixture
genomic dna
kcl
tris
1x pcr buffer
gotaq dna polymerase

Images

1) Product Images from "Population Abundance of Potentially Pathogenic Organisms in Intestinal Microbiome of Jungle Crow (Corvus macrorhynchos) Shown with 16S rRNA Gene-Based Microbial Community Analysis"

Article Title: Population Abundance of Potentially Pathogenic Organisms in Intestinal Microbiome of Jungle Crow (Corvus macrorhynchos) Shown with 16S rRNA Gene-Based Microbial Community Analysis

Journal: BioMed Research International

doi: 10.1155/2013/438956

Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

Figure Legend Snippet: Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

Techniques Used: Amplification, Polymerase Chain Reaction, Marker

Amplification:

Article Title: Non-Redundant and Overlapping Oncogenic Readouts of Non-Canonical and Novel Colorectal Cancer KRAS and NRAS Mutants
Article Snippet: .. The mutant KRAS and NRAS variant fragments were amplified in a polymerase chain reaction (PCR) mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer, Clontech Laboratories, Inc., Mountainview, CA, USA), 0.125 μM of each deoxynucleoside triphosphate (Promega, Madison, WI, USA), 2 μM each of the forward and reverse primers, 1U Taq polymerase (Titanium® Taq polymerase, Clontech Laboratories, Inc.), and 50 ng of the pTargeT-WT KRAS or pTargeT-WT NRAS template. .. Amplification was performed with an initial denaturation temperature of 94 °C for 5 min, followed by 25–30 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, with a final extension step at 72 °C for 10 min.

Mutagenesis:

Isolation:

Article Title: Population Abundance of Potentially Pathogenic Organisms in Intestinal Microbiome of Jungle Crow (Corvus macrorhynchos) Shown with 16S rRNA Gene-Based Microbial Community Analysis
Article Snippet: .. PCR Conditions Twenty-five to fifty nanograms of isolated DNA were transferred to a PCR tube containing 50 μ L of a polymerase chain reaction (PCR) mixture containing LA Taq DNA polymerase (TaKaRa Bio, Shiga, Japan). .. For DNA evaluation, the eubacterial 16S rRNA gene sequence intervened between UNIV341F (5′-CCTACGGGAGGCAGCAG-3′) and UNIV907R (5′-CCCCGTCAATTCCTTTGAGTTT-3′) was amplified.

Concentration Assay:

Polymerase Chain Reaction:

Article Title: Evidence of Subterranean Termite Feeding Deterrent Produced by Brown Rot Fungus Fibroporia radiculosa (Peck) Parmasto 1968 (Polyporales, Fomitopsidaceae)
Article Snippet: .. The polymerase chain reaction (PCR) mixture was prepared using TaKaRa Ex Taq (TaKaRa Bio, Shiga, Japan), and primers for ITS1 (5’-TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC) were added. .. PCR was performed with a 2720 Thermal Cycler (Applied Biosystems, Foster City, CA, USA) with the following parameters: initial denaturation for 3 min at 94 °C; 25 cycles of denaturation for 15 s at 94 °C, annealing for 30 s at 55 °C, and extension for 30 s at 72 °C; and a final extension for 5 min at 72 °C.

Article Title: Taxonomy of myid bivalves from fragmented brackish-water habitats in India, with a description of a new genus Indosphenia ( Myidae, Myoidea, Myidae)
Article Snippet: .. The polymerase chain reaction ( PCR ) mixture consisted of 25 μL Master Mix (Takara Clontech EmeraldAmp® GT PCR Master Mix), 1 μL forward primer, 1 μL reverse primer, 8 μL template DNA, and 15 μL distilled deionised water. .. The amplification primers were LCO-1490 F (5'- GGTCAACAAATCATAAAGATATTGG-3') and HCO-2198 R (5'-TAAACTTCAGGGTGACCAAAAAATCA-3'), used for amplifying mitochondrial cytochrome c oxidase subunit I ( mtCOI ) gene sequences ( ).

Article Title: Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells
Article Snippet: .. Polymerase chain reaction (PCR) mixture was from Takara Biotechnology Co., Ltd., (Dalian, China). .. Lipofectamine™ 2000 reagent was obtained from Invitrogen (Carlsbad, CA, USA).

Article Title: Isolation and 2,4-D-degrading characteristics of Cupriaviduscampinensis BJ71
Article Snippet: .. Each polymerase chain reaction (PCR) mixture contained 1 × Premix Taq (Ex Taq Version, Takara Bio, Shiga, Japan), 0.4 μM each primer, and ~50 ng template DNA. .. The PCR amplification conditions were as follows: 95 °C for 5 min; followed by 35 cycles of 95 °C for 45 s, 55 °C for 45 s, and 72 °C for 90 s. To ensure complete elongation, a final step of 72 °C for 7 min was performed.

Article Title: Effects of Dietary Supplementation With Enterococcus faecium and Clostridium butyricum, Either Alone or in Combination, on Growth and Fecal Microbiota Composition of Post-weaning Pigs at a Commercial Farm
Article Snippet: .. Each 50 μL of polymerase chain reaction (PCR) mixture contained 1 μL of sample DNA, 21 μL of MilliQ® water, 25 μL of 2X MightyAmp® Buffer, 1 μL of forward primer (5 μM), 1 μL of reverse primer (5 μM), and 1 μL (1.25 U) of MightyAmp DNA Polymerase (Takara, Tokyo, Japan). ..

Article Title: Use of RNAi technology to develop a PRSV-resistant transgenic papaya
Article Snippet: .. The polymerase chain reaction (PCR) mixture contained 1 µl of cDNA, 0.5 µl of each primer (10 µM), 2 µl of 10 × PCR buffer, 1 µl of dNTP, 0.25 U of Taq DNA polymerase (Takara), and nuclease-free water to a final volume of 20 µl. .. The amplification consisted of 35 cycles at 94 °C (30 s), 50 °C (1 min), and 72 °C (1 min); the initial cycle incorporated a melting step of 94 °C for 3 min and the final cycle a synthesis step of 72 °C for 10 min. PCR products were electrophoresed on 1.5% agarose gel, and visualized after ethidium bromide staining.