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TaKaRa polymerase chain reaction pcr mixture
Polymerase Chain Reaction Pcr Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr mixture/product/TaKaRa
Average 90 stars, based on 31 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr mixture - by Bioz Stars, 2021-01
90/100 stars

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Amplification:

Article Title: Non-Redundant and Overlapping Oncogenic Readouts of Non-Canonical and Novel Colorectal Cancer KRAS and NRAS Mutants
Article Snippet: .. The mutant KRAS and NRAS variant fragments were amplified in a polymerase chain reaction (PCR) mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer, Clontech Laboratories, Inc., Mountainview, CA, USA), 0.125 μM of each deoxynucleoside triphosphate (Promega, Madison, WI, USA), 2 μM each of the forward and reverse primers, 1U Taq polymerase (Titanium® Taq polymerase, Clontech Laboratories, Inc.), and 50 ng of the pTargeT-WT KRAS or pTargeT-WT NRAS template. .. Amplification was performed with an initial denaturation temperature of 94 °C for 5 min, followed by 25–30 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, with a final extension step at 72 °C for 10 min.

Mutagenesis:

Article Title: Non-Redundant and Overlapping Oncogenic Readouts of Non-Canonical and Novel Colorectal Cancer KRAS and NRAS Mutants
Article Snippet: .. The mutant KRAS and NRAS variant fragments were amplified in a polymerase chain reaction (PCR) mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer, Clontech Laboratories, Inc., Mountainview, CA, USA), 0.125 μM of each deoxynucleoside triphosphate (Promega, Madison, WI, USA), 2 μM each of the forward and reverse primers, 1U Taq polymerase (Titanium® Taq polymerase, Clontech Laboratories, Inc.), and 50 ng of the pTargeT-WT KRAS or pTargeT-WT NRAS template. .. Amplification was performed with an initial denaturation temperature of 94 °C for 5 min, followed by 25–30 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, with a final extension step at 72 °C for 10 min.

Purification:

Article Title: Chemical, Microbiological, and Functional Characterization of Kefir Produced from Cow's Milk and Soy Milk
Article Snippet: .. A total of 30 μ L polymerase chain reaction (PCR) mixture contained 3 μ L buffer solution, 2.4 μ L dNTPs, 1.5 μ L of each primer, 0.15 μ L Ex Taq polymerase (Takara, Shiga, Japan), 20.85 μ L purified water, and 0.6 μ L of the extracted DNA. .. The PCR amplification was carried out as follows: for bacteria, template DNA was denatured for 2 min at 96°C, followed by 25 cycles of denaturing at 96°C for 15 s, annealing at 50°C for 15 s, and primer extension at 72°C for 1 min 30 s; for yeasts, template DNA was denatured for 2 min at 96°C, followed by 25 cycles of denaturing at 96°C for 15 s, annealing at 50°C for 15 s, and primer extension at 72°C for 40 s. The amplification products were analysed by electrophoresis on 1.0% agarose gels before being sequenced by Eurofins Genomics Co. Ltd. (Tokyo, Japan).

SYBR Green Assay:

Article Title: Applicability of Hyaluronic Acid-Alginate Hydrogel and Ovarian Cells for In Vitro Development of Mouse Preantral Follicles
Article Snippet: .. The polymerase chain reaction (PCR) mix for each well contained 5 ml Power SYBR Green PCR Master Mix (Takara, Japan), 1 ml dH2 O, 1 ml of each of the forward and reverse primers (5 pmol/ml), and 2 ml of single-strand cDNA in a final reaction volume of 10 ml. .. PCR was performed on the ABI StepOnePlus real-time PCR system (Applied Biosystems) using the following program: stage 1: 95˚C for 10 minutes; stage 2: 40 cycles of 95˚C for 15 seconds and 60˚C for 1 minute; and stage 3: 95˚C for 15 seconds, 60˚C for 15 seconds, and 95˚C for 15 seconds.

Concentration Assay:

Article Title: Assessment and molecular characterization of Bacillus cereus isolated from edible fungi in China
Article Snippet: .. The polymerase chain reaction (PCR) mixture (25 μL) comprised of 50 ng genomic DNA, 0.5 μL of each primer solution (concentration 10 μM), and 12.5 μL PCR Premix Taq™ (Takara, China). .. The amplification process included: initial denaturation at 94 °C for 5 min, 30 cycles of 94 °C for 1 min, 55 °C or 58 °C (58 °C for cesB and 55 °C for the other toxin genes) for 1 min, 72 °C for 1 min, and a final 10 min extension at 72 °C [ , , , ].

Article Title: Non-Redundant and Overlapping Oncogenic Readouts of Non-Canonical and Novel Colorectal Cancer KRAS and NRAS Mutants
Article Snippet: .. The mutant KRAS and NRAS variant fragments were amplified in a polymerase chain reaction (PCR) mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer, Clontech Laboratories, Inc., Mountainview, CA, USA), 0.125 μM of each deoxynucleoside triphosphate (Promega, Madison, WI, USA), 2 μM each of the forward and reverse primers, 1U Taq polymerase (Titanium® Taq polymerase, Clontech Laboratories, Inc.), and 50 ng of the pTargeT-WT KRAS or pTargeT-WT NRAS template. .. Amplification was performed with an initial denaturation temperature of 94 °C for 5 min, followed by 25–30 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, with a final extension step at 72 °C for 10 min.

Polymerase Chain Reaction:

Article Title: Genetic variability of the Aedes aegypti (Diptera: Culicidae) mosquito in El Salvador, vector of dengue, yellow fever, chikungunya and Zika
Article Snippet: .. A polymerase chain reaction (PCR) mix for six samples consisted of the following: 195.6 μl sterile ultra-pure water; 2.4 μl Taq polymerase (Clonetech, Mountainview, CA, USA); 30 μl Taq 10× buffer; 24 μl dNTPs; 6 μl forward primer; and 6 μl reverse primer and 2 μl template DNA (~100 ng template). ..

Article Title: Chemical, Microbiological, and Functional Characterization of Kefir Produced from Cow's Milk and Soy Milk
Article Snippet: .. A total of 30 μ L polymerase chain reaction (PCR) mixture contained 3 μ L buffer solution, 2.4 μ L dNTPs, 1.5 μ L of each primer, 0.15 μ L Ex Taq polymerase (Takara, Shiga, Japan), 20.85 μ L purified water, and 0.6 μ L of the extracted DNA. .. The PCR amplification was carried out as follows: for bacteria, template DNA was denatured for 2 min at 96°C, followed by 25 cycles of denaturing at 96°C for 15 s, annealing at 50°C for 15 s, and primer extension at 72°C for 1 min 30 s; for yeasts, template DNA was denatured for 2 min at 96°C, followed by 25 cycles of denaturing at 96°C for 15 s, annealing at 50°C for 15 s, and primer extension at 72°C for 40 s. The amplification products were analysed by electrophoresis on 1.0% agarose gels before being sequenced by Eurofins Genomics Co. Ltd. (Tokyo, Japan).

Article Title: Applicability of Hyaluronic Acid-Alginate Hydrogel and Ovarian Cells for In Vitro Development of Mouse Preantral Follicles
Article Snippet: .. The polymerase chain reaction (PCR) mix for each well contained 5 ml Power SYBR Green PCR Master Mix (Takara, Japan), 1 ml dH2 O, 1 ml of each of the forward and reverse primers (5 pmol/ml), and 2 ml of single-strand cDNA in a final reaction volume of 10 ml. .. PCR was performed on the ABI StepOnePlus real-time PCR system (Applied Biosystems) using the following program: stage 1: 95˚C for 10 minutes; stage 2: 40 cycles of 95˚C for 15 seconds and 60˚C for 1 minute; and stage 3: 95˚C for 15 seconds, 60˚C for 15 seconds, and 95˚C for 15 seconds.

Article Title: Assessment and molecular characterization of Bacillus cereus isolated from edible fungi in China
Article Snippet: .. The polymerase chain reaction (PCR) mixture (25 μL) comprised of 50 ng genomic DNA, 0.5 μL of each primer solution (concentration 10 μM), and 12.5 μL PCR Premix Taq™ (Takara, China). .. The amplification process included: initial denaturation at 94 °C for 5 min, 30 cycles of 94 °C for 1 min, 55 °C or 58 °C (58 °C for cesB and 55 °C for the other toxin genes) for 1 min, 72 °C for 1 min, and a final 10 min extension at 72 °C [ , , , ].

Article Title: Non-Redundant and Overlapping Oncogenic Readouts of Non-Canonical and Novel Colorectal Cancer KRAS and NRAS Mutants
Article Snippet: .. The mutant KRAS and NRAS variant fragments were amplified in a polymerase chain reaction (PCR) mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer, Clontech Laboratories, Inc., Mountainview, CA, USA), 0.125 μM of each deoxynucleoside triphosphate (Promega, Madison, WI, USA), 2 μM each of the forward and reverse primers, 1U Taq polymerase (Titanium® Taq polymerase, Clontech Laboratories, Inc.), and 50 ng of the pTargeT-WT KRAS or pTargeT-WT NRAS template. .. Amplification was performed with an initial denaturation temperature of 94 °C for 5 min, followed by 25–30 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, with a final extension step at 72 °C for 10 min.

Article Title: A Comprehensive Census of Microbial Diversity in Hot Springs of Tengchong, Yunnan Province China Using 16S rRNA Gene Pyrosequencing
Article Snippet: .. The polymerase chain reaction (PCR) mix contained 10 ng of template DNA, 5 µl rTaq reaction buffer, 400 nM of each primer, 200 µM dNTPs, and 0.3 unit of rTaq polymerase (Takara, Dalian, China) in a 25 µl reaction. .. The amplification procedure was as follows: an initial denaturation step at 95°C for 5 min, and 30 cycles of denaturing at 94°C for 30 s, annealing at 54°C for 30 s, and extension at 72°C for 1 min, followed by a final extension step at 72°C for 10 min. Amplicons from four PCR were pooled for each sample.

Article Title: TissueGene-C promotes an anti-inflammatory micro-environment in a rat monoiodoacetate model of osteoarthritis via polarization of M2 macrophages leading to pain relief and structural improvement
Article Snippet: .. The polymerase chain reaction (PCR) mixture was prepared to a final volume of 50 µL, as follows: 1 µL cDNA, 0.2 µM of each primer, 10 µL SYBR Premix Ex Taq (TAKARA Bio, Shiga, Japan). .. Expression levels of the target genes were quantified with the ABI 7900 real-time PCR system (RT-PCR, Applied Biosystems, CA, USA).

Article Title: Effects of Dietary Supplementation With Enterococcus faecium and Clostridium butyricum, Either Alone or in Combination, on Growth and Fecal Microbiota Composition of Post-weaning Pigs at a Commercial Farm
Article Snippet: .. Each 50 μL of polymerase chain reaction (PCR) mixture contained 1 μL of sample DNA, 21 μL of MilliQ® water, 25 μL of 2X MightyAmp® Buffer, 1 μL of forward primer (5 μM), 1 μL of reverse primer (5 μM), and 1 μL (1.25 U) of MightyAmp DNA Polymerase (Takara, Tokyo, Japan). ..

Variant Assay:

Article Title: Non-Redundant and Overlapping Oncogenic Readouts of Non-Canonical and Novel Colorectal Cancer KRAS and NRAS Mutants
Article Snippet: .. The mutant KRAS and NRAS variant fragments were amplified in a polymerase chain reaction (PCR) mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer, Clontech Laboratories, Inc., Mountainview, CA, USA), 0.125 μM of each deoxynucleoside triphosphate (Promega, Madison, WI, USA), 2 μM each of the forward and reverse primers, 1U Taq polymerase (Titanium® Taq polymerase, Clontech Laboratories, Inc.), and 50 ng of the pTargeT-WT KRAS or pTargeT-WT NRAS template. .. Amplification was performed with an initial denaturation temperature of 94 °C for 5 min, followed by 25–30 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, with a final extension step at 72 °C for 10 min.

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  • 92
    TaKaRa tb green pcr master mix
    A , Quantitative <t>PCR</t> (qPCR) analysis of DONSON mRNA amount in HeLa cells. Total RNA was isolated from HeLa cells after 48 h treatment of the indicated siRNAs, and was subjected to reverse transcription. The resulting <t>cDNA</t> was subjected to real-time qPCR analysis. B, Time-lapse observation of DONSON localization at different cell cycle stages. HeLa GFP-DONSON cells were visualized every 10 min for 24 h after 24 h treatment of 1 μg/mL Doxycycline and 3 h treatment of 100 nM SiR-DNA. DNA (blue) and GFP-DONSON (green). Arrowheads indicate GFP-DONSON foci. C, The centrosomal localization of DONSON in mitosis. HeLa GFP-DONSON cells were fixed after 24 h treatment of 1 μg/mL Doxycycline and were immunostained with antibodies against GFP (green) and CP110 (red). D, Phenotype rescue experiments by expression of RNAi-resistant (RNAi-R) form of full-length (FL) or nuclear localization signal deleted (ΔNLS) DONSON. HeLa cells were treated with siControl or siDONSON, followed by transfection with FLAG empty (control), RNAi-resistant DONSON-FL, or DONSON-ΔNLS. The cells were immunostained with antibodies against FLAG (green) and Cep192 (red). Arrowheads indicate Cep192 foci. E, Histograms represent frequency of interphase cells with the indicated phenotypes observed in D. Values are mean percentages ± s.d. from three independent experiments (n = 30 for each experiment). Tukey’s multiple comparisons test was used in E to obtain P value. **, p
    Tb Green Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tb green pcr master mix/product/TaKaRa
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tb green pcr master mix - by Bioz Stars, 2021-01
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    92
    TaKaRa dna template
    Inverse <t>PCR</t> (iPCR) and sequencing of the boundary region surrounding the Mk + 1.3 locus. ( a ) Agarose gel electrophoresis of nested PCR products stained with ethidium bromide. The gel is the full length gel and lanes have not been cropped or stitched. ( b ) Chromatograms generated from direct sequencing of the iPCR products. Black and red letters above the chromatograms indicate the sequences of chromosomal positions chr 7: 62,416,891–62,416,891 and chr 7: 62,268,615–62,268,638 (on the University of California, Santa Cruz, genome browser (GRCm38/mm10)), respectively. W: wild-type <t>DNA,</t> Tg: hemizygous transgenic DNA, M: molecular size marker.
    Dna Template, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 528 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna template/product/TaKaRa
    Average 92 stars, based on 528 article reviews
    Price from $9.99 to $1999.99
    dna template - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    92
    TaKaRa pcr reaction mixture
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Pcr Reaction Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr reaction mixture/product/TaKaRa
    Average 92 stars, based on 421 article reviews
    Price from $9.99 to $1999.99
    pcr reaction mixture - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    92
    TaKaRa pcr master mix
    Alternative PNRC2 transcripts caused by a chimpanzee-specific ERV. (A) Using the UCSC Genome Browser, the PNRC2 gene is anatomized into intron, un-translated region (UTR), and coding exon. (B) The structures of chimpanzee and non-chimpanzee primate PNRC2 s and their transcripts are depicted. The black and gray boxes indicate an exon and UTRs, respectively, and the red box denotes the untranscribed UTR1 of the chimpanzee PNRC2 . Four different primers used to amplify <t>PCR</t> products are shown in red, blue, green, and black arrows, respectively. The target site duplications (TSDs) of the full-length chimpanzee-specific ERV are written in red. (C) The picture shows an agarose gel image of PCR products. Genomic DNAs from four different primates (human, chimpanzee, gorilla, and orangutan) were used as a <t>DNA</t> template for each PCR reaction.
    Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/TaKaRa
    Average 92 stars, based on 128 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2021-01
    92/100 stars
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    A , Quantitative PCR (qPCR) analysis of DONSON mRNA amount in HeLa cells. Total RNA was isolated from HeLa cells after 48 h treatment of the indicated siRNAs, and was subjected to reverse transcription. The resulting cDNA was subjected to real-time qPCR analysis. B, Time-lapse observation of DONSON localization at different cell cycle stages. HeLa GFP-DONSON cells were visualized every 10 min for 24 h after 24 h treatment of 1 μg/mL Doxycycline and 3 h treatment of 100 nM SiR-DNA. DNA (blue) and GFP-DONSON (green). Arrowheads indicate GFP-DONSON foci. C, The centrosomal localization of DONSON in mitosis. HeLa GFP-DONSON cells were fixed after 24 h treatment of 1 μg/mL Doxycycline and were immunostained with antibodies against GFP (green) and CP110 (red). D, Phenotype rescue experiments by expression of RNAi-resistant (RNAi-R) form of full-length (FL) or nuclear localization signal deleted (ΔNLS) DONSON. HeLa cells were treated with siControl or siDONSON, followed by transfection with FLAG empty (control), RNAi-resistant DONSON-FL, or DONSON-ΔNLS. The cells were immunostained with antibodies against FLAG (green) and Cep192 (red). Arrowheads indicate Cep192 foci. E, Histograms represent frequency of interphase cells with the indicated phenotypes observed in D. Values are mean percentages ± s.d. from three independent experiments (n = 30 for each experiment). Tukey’s multiple comparisons test was used in E to obtain P value. **, p

    Journal: bioRxiv

    Article Title: DONSON, a gene responsible for microcephalic primordial dwarfism, ensures proper centriole duplication cycle by maintaining centriole engagement during interphase

    doi: 10.1101/2020.05.10.086777

    Figure Lengend Snippet: A , Quantitative PCR (qPCR) analysis of DONSON mRNA amount in HeLa cells. Total RNA was isolated from HeLa cells after 48 h treatment of the indicated siRNAs, and was subjected to reverse transcription. The resulting cDNA was subjected to real-time qPCR analysis. B, Time-lapse observation of DONSON localization at different cell cycle stages. HeLa GFP-DONSON cells were visualized every 10 min for 24 h after 24 h treatment of 1 μg/mL Doxycycline and 3 h treatment of 100 nM SiR-DNA. DNA (blue) and GFP-DONSON (green). Arrowheads indicate GFP-DONSON foci. C, The centrosomal localization of DONSON in mitosis. HeLa GFP-DONSON cells were fixed after 24 h treatment of 1 μg/mL Doxycycline and were immunostained with antibodies against GFP (green) and CP110 (red). D, Phenotype rescue experiments by expression of RNAi-resistant (RNAi-R) form of full-length (FL) or nuclear localization signal deleted (ΔNLS) DONSON. HeLa cells were treated with siControl or siDONSON, followed by transfection with FLAG empty (control), RNAi-resistant DONSON-FL, or DONSON-ΔNLS. The cells were immunostained with antibodies against FLAG (green) and Cep192 (red). Arrowheads indicate Cep192 foci. E, Histograms represent frequency of interphase cells with the indicated phenotypes observed in D. Values are mean percentages ± s.d. from three independent experiments (n = 30 for each experiment). Tukey’s multiple comparisons test was used in E to obtain P value. **, p

    Article Snippet: Reverse transcription and real-time qPCR Total RNA (1 μg) isolated from cells with the use of TRIZOL reagent (Thermo Fischer Scientific) was subject to reverse transcription using Quantiscript Reverse Transcription Kit (QIAGEN), and the resulting cDNA was subjected to real-time qPCR analysis with TB Green PCR Master Mix (TaKaRa) and specific primers in a StepOnePlus Real-Time PCR system (Applied Biosystems).

    Techniques: Real-time Polymerase Chain Reaction, Isolation, Expressing, Transfection

    Inverse PCR (iPCR) and sequencing of the boundary region surrounding the Mk + 1.3 locus. ( a ) Agarose gel electrophoresis of nested PCR products stained with ethidium bromide. The gel is the full length gel and lanes have not been cropped or stitched. ( b ) Chromatograms generated from direct sequencing of the iPCR products. Black and red letters above the chromatograms indicate the sequences of chromosomal positions chr 7: 62,416,891–62,416,891 and chr 7: 62,268,615–62,268,638 (on the University of California, Santa Cruz, genome browser (GRCm38/mm10)), respectively. W: wild-type DNA, Tg: hemizygous transgenic DNA, M: molecular size marker.

    Journal: Scientific Reports

    Article Title: Application of droplet digital PCR in the analysis of genome integration and organization of the transgene in BAC transgenic mice

    doi: 10.1038/s41598-018-25001-x

    Figure Lengend Snippet: Inverse PCR (iPCR) and sequencing of the boundary region surrounding the Mk + 1.3 locus. ( a ) Agarose gel electrophoresis of nested PCR products stained with ethidium bromide. The gel is the full length gel and lanes have not been cropped or stitched. ( b ) Chromatograms generated from direct sequencing of the iPCR products. Black and red letters above the chromatograms indicate the sequences of chromosomal positions chr 7: 62,416,891–62,416,891 and chr 7: 62,268,615–62,268,638 (on the University of California, Santa Cruz, genome browser (GRCm38/mm10)), respectively. W: wild-type DNA, Tg: hemizygous transgenic DNA, M: molecular size marker.

    Article Snippet: Nested amplification around the circular DNA using primers oriented in a direction opposite to that of conventional PCR was performed using reaction mixtures containing DNA template, 1 × Ex Taq buffer (containing 2.0 mM of MgCl2 ), 250 μM of dNTP mix, 0.5 μM of each primer, and 0.25 U/μl of Ex Taq DNA Polymerase (Takara Bio, Otsu, Japan).

    Techniques: Inverse PCR, Sequencing, Agarose Gel Electrophoresis, Nested PCR, Staining, Generated, Transgenic Assay, Marker

    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by SYBR-Green I real-time PCR. The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.

    Journal: Oncology Letters

    Article Title: Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer

    doi: 10.3892/ol.2012.714

    Figure Lengend Snippet: Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by SYBR-Green I real-time PCR. The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.

    Article Snippet: The 25 μl PCR reaction mixture included 1× SYBR premix Ex Taq mix (Takara), 2 μl RT products and 10 nM of each forward and reverse primer.

    Techniques: Plasmid Preparation, SYBR Green Assay, Real-time Polymerase Chain Reaction, Produced

    Alternative PNRC2 transcripts caused by a chimpanzee-specific ERV. (A) Using the UCSC Genome Browser, the PNRC2 gene is anatomized into intron, un-translated region (UTR), and coding exon. (B) The structures of chimpanzee and non-chimpanzee primate PNRC2 s and their transcripts are depicted. The black and gray boxes indicate an exon and UTRs, respectively, and the red box denotes the untranscribed UTR1 of the chimpanzee PNRC2 . Four different primers used to amplify PCR products are shown in red, blue, green, and black arrows, respectively. The target site duplications (TSDs) of the full-length chimpanzee-specific ERV are written in red. (C) The picture shows an agarose gel image of PCR products. Genomic DNAs from four different primates (human, chimpanzee, gorilla, and orangutan) were used as a DNA template for each PCR reaction.

    Journal: PLoS ONE

    Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome

    doi: 10.1371/journal.pone.0101195

    Figure Lengend Snippet: Alternative PNRC2 transcripts caused by a chimpanzee-specific ERV. (A) Using the UCSC Genome Browser, the PNRC2 gene is anatomized into intron, un-translated region (UTR), and coding exon. (B) The structures of chimpanzee and non-chimpanzee primate PNRC2 s and their transcripts are depicted. The black and gray boxes indicate an exon and UTRs, respectively, and the red box denotes the untranscribed UTR1 of the chimpanzee PNRC2 . Four different primers used to amplify PCR products are shown in red, blue, green, and black arrows, respectively. The target site duplications (TSDs) of the full-length chimpanzee-specific ERV are written in red. (C) The picture shows an agarose gel image of PCR products. Genomic DNAs from four different primates (human, chimpanzee, gorilla, and orangutan) were used as a DNA template for each PCR reaction.

    Article Snippet: We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of PCR master mix containing DNA polymerase (TaKaRa EmeraldAmp GT PCR Master Mix, TaKaRa, Japan).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis