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Kapa Biosystems polymerase chain reaction pcr mix
Polymerase Chain Reaction Pcr Mix, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr mix/product/Kapa Biosystems
Average 89 stars, based on 3 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr mix - by Bioz Stars, 2020-08
89/100 stars

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Polymerase Chain Reaction:

Article Title: Synthetic biosensors for precise gene control and real-time monitoring of metabolites
Article Snippet: .. Polymerase chain reaction (PCR) mix was purchased from Kapa Biosystems (Wilmington, MA, USA). ..

Article Title: Probiotic Lactobacillus casei: Effective for Managing Childhood Diarrhea by Altering Gut Microbiota and Attenuating Fecal Inflammatory Markers
Article Snippet: .. 16 S rRNA Amplification For each fecal sample, a 50 μL polymerase chain reaction (PCR) mix was prepared containing 25 ng DNA template, 5X KAPA HiFi Buffer, 10 mM KAPA dNTPMix, 1 U/μL KAPA HiFi DNA Polymerase (KAPA Biosystem, Boston, United States), and 0.3 μM of each primer (Tri-I, New Taipei, Taiwan). .. PCR reaction conditions consisted of an initial 95 °C for 3 min followed by 15–25 cycles of 98 °C for 20 sec, 45 °C for 15 sec, and 72 °C for 15 sec, and a final extension of 72 °C for 1 min.

Article Title: A trade off between catalytic activity and protein stability determines the clinical manifestations of glucose-6-phosphate dehydrogenase (G6PD) deficiency
Article Snippet: .. The polymerase chain reaction (PCR) mixture (50 μL) comprised 1 × KAPA HiFi reaction buffer, 50 ng of template plasmid, 100 ng of forward and reverse primers, 0.3 μM of each dNTP mix, and 1 U of KAPA HiFi DNA polymerase (Kapa Biosystems); the primers used to generate the G6PD variants are listed in . .. The cycling parameters for site-directed mutagenesis are as follows: 1 cycle of 95 °C for 5 min and 16 cycles of 98 °C for 20 s, 55 °C for 15 s, and 68 °C for 3 min and 30 s. The PCR products of site-directed mutagenesis went through Dpn I digestion at 37 °C for 2 h to digest the parental DNA.

Article Title: Microbial changes linked to the accelerated degradation of the herbicide atrazine in a range of temperate soils
Article Snippet: .. The polymerase chain reaction (PCR) mix consisted of 1× KAPA HiFi fidelity buffer (Kapa Biosystems, Woburn, MA, USA), 0.3 μM of dNTPs, 0.3 μM of each primer (Table ), 1 U μL−1 KAPA HiFi polymerase and nuclease-free water (Severn Biotech Ltd., Worcestershire, UK) to reach 25 μL final volume. .. The PCR followed these thermal cycling conditions: initial denaturation at 95 °C for 5 min and 30 cycles of denaturation at 98 °C for 30 s, annealing for 15 s (at the specified temperature in Table ) and 15 s elongation at 72 °C, followed by a final extension of 5 min at 72 °C (Bio-Rad Laboratories, Inc., USA).

Amplification:

Article Title: Probiotic Lactobacillus casei: Effective for Managing Childhood Diarrhea by Altering Gut Microbiota and Attenuating Fecal Inflammatory Markers
Article Snippet: .. 16 S rRNA Amplification For each fecal sample, a 50 μL polymerase chain reaction (PCR) mix was prepared containing 25 ng DNA template, 5X KAPA HiFi Buffer, 10 mM KAPA dNTPMix, 1 U/μL KAPA HiFi DNA Polymerase (KAPA Biosystem, Boston, United States), and 0.3 μM of each primer (Tri-I, New Taipei, Taiwan). .. PCR reaction conditions consisted of an initial 95 °C for 3 min followed by 15–25 cycles of 98 °C for 20 sec, 45 °C for 15 sec, and 72 °C for 15 sec, and a final extension of 72 °C for 1 min.

Plasmid Preparation:

Article Title: A trade off between catalytic activity and protein stability determines the clinical manifestations of glucose-6-phosphate dehydrogenase (G6PD) deficiency
Article Snippet: .. The polymerase chain reaction (PCR) mixture (50 μL) comprised 1 × KAPA HiFi reaction buffer, 50 ng of template plasmid, 100 ng of forward and reverse primers, 0.3 μM of each dNTP mix, and 1 U of KAPA HiFi DNA polymerase (Kapa Biosystems); the primers used to generate the G6PD variants are listed in . .. The cycling parameters for site-directed mutagenesis are as follows: 1 cycle of 95 °C for 5 min and 16 cycles of 98 °C for 20 s, 55 °C for 15 s, and 68 °C for 3 min and 30 s. The PCR products of site-directed mutagenesis went through Dpn I digestion at 37 °C for 2 h to digest the parental DNA.

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  • 91
    Kapa Biosystems qpcr reaction mixture
    COR388 target engagement and dose-dependent effects on brain P. gingivalis , Aβ 1–42 , and TNFα in mice. ( A ) COR553 fluorescent activity probe for Kgp. ( B ) COR553 labeling of Kgp in P. gingivalis W83 strain and no labeling in mutant deficient in Kgp (ΔKgp). ( C ) W83 lysates labeled with COR553. Left lane, before immunodepletion; middle lane, after immunodepletion with anti-Kgp–conjugated beads; right lane, after elution from anti-Kgp–conjugated beads. ( D ) W83 strain titrated and labeled with COR553 to determine the limit of bacterial detection. See Results for details. ( E ) Oral plaque samples from human subjects (CB1-5) with periodontal disease were incubated ex vivo with COR553 probe with or without preincubation with COR388. COR553 probe and CAB102 detected Kgp strongly in three subjects (CB1, CB4, and CB5) and weakly in one subject (CB3). COR388 preincubation blocked COR553 probe binding to Kgp. ( F ) <t>qPCR</t> analysis of plaque samples using hmuY gene–specific primers identified P. gingivalis <t>DNA</t> in samples. ( G ) qPCR analysis of saliva samples. The bar graphs in (F) and (G) show the means and SEMs of three replicates. ( H ) COR388 treatment of W83 culture in defined growth medium reduced growth similarly to a Kgp-deficient strain (ΔKgp) over 43 hours. ( I ) Resistance developed rapidly to moxifloxacin but not COR388 with repeat passaging of bacterial culture. ( J to L ) Efficacy of COR388 at three oral doses of 3, 10, and 30 mg/kg twice daily in treating an established P. gingivalis brain infection in mice. Reduction of brain tissue levels of P. gingivalis (J), Aβ 1–42 (K), and TNFα (L). The bar graphs show the means with SEM error bars. *** P
    Qpcr Reaction Mixture, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qpcr reaction mixture/product/Kapa Biosystems
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    qpcr reaction mixture - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    93
    Roche universal qpcr mix
    Expression of 62 activation-related host genes in CB or AB mDC inoculated with rgRSV or IAV. CB or AB mDC ( n = 3 different CB or AB donors) were mock inoculated, inoculated with rgRSV or IAV at an MOI of 3 PFU/cell, or stimulated with 1 μg/ml of LPS. At 24 hpi, total cellular RNA was prepared and reverse transcribed using random primers. The <t>cDNA</t> was analyzed by <t>qPCR</t> using a custom-made 62-gene TaqMan array. qPCR results were analyzed using the comparative threshold cycle (ΔΔ C T ) method and normalized to the amount of 18S rRNA. The results are presented as the fold increase (red) or decrease (green) (in log 2 ) in gene expression over that for mock-inoculated cells from the same donor. Treg, regulatory T cells.
    Universal Qpcr Mix, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/universal qpcr mix/product/Roche
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    universal qpcr mix - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Kapa Biosystems qpcr master mix
    Effects of DEX on interaction between NeuroD1 and E-box on Pomc promoter in AtT20 cells. (A) Effect of of DEX on interaction between NeuroD1 and E-box on Pomc promoter examined by ChIP assay using E-box primer. ChIP assay was performed using digested chromatin extracted from the cells cultured in the presence (100 nM) or absence (control) of DEX for 30 min (B), 60 min (C), or 24 hrs (D). Chromatin fragments were <t>immunoprecipitated</t> either by normal rabbit IgG (negative control) or NeuroD1 antibody. Purified DNA was analyzed by <t>qPCR</t> using primers specific for E-box containing sequence on Pomc promoter. The expected size of E-box is 196 bp. Few qPCR products observed in the input samples were detected in the immunoprecipitation using normal IgG. Immunoprecipitated DNA was quantified by qPCR and normalized to the values obtained after amplification of unprecipitated 1% input DNA. Each point represents mean ± SEM (n = 3). **P
    Qpcr Master Mix, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qpcr master mix/product/Kapa Biosystems
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    qpcr master mix - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    85
    Kapa Biosystems quantitative pcr qpcr mix
    p30 decreases TRL3 and TRL4 but not TLR7/8 signaling. (A to C) Real-time quantitative <t>PCR</t> analysis of type 1 ISGs. THP-1 cells were transduced with p30 or control lentivirus, kept in culture for 72 h, and stimulated for 6 h with poly(I·C) (A),
    Quantitative Pcr Qpcr Mix, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative pcr qpcr mix/product/Kapa Biosystems
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative pcr qpcr mix - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    COR388 target engagement and dose-dependent effects on brain P. gingivalis , Aβ 1–42 , and TNFα in mice. ( A ) COR553 fluorescent activity probe for Kgp. ( B ) COR553 labeling of Kgp in P. gingivalis W83 strain and no labeling in mutant deficient in Kgp (ΔKgp). ( C ) W83 lysates labeled with COR553. Left lane, before immunodepletion; middle lane, after immunodepletion with anti-Kgp–conjugated beads; right lane, after elution from anti-Kgp–conjugated beads. ( D ) W83 strain titrated and labeled with COR553 to determine the limit of bacterial detection. See Results for details. ( E ) Oral plaque samples from human subjects (CB1-5) with periodontal disease were incubated ex vivo with COR553 probe with or without preincubation with COR388. COR553 probe and CAB102 detected Kgp strongly in three subjects (CB1, CB4, and CB5) and weakly in one subject (CB3). COR388 preincubation blocked COR553 probe binding to Kgp. ( F ) qPCR analysis of plaque samples using hmuY gene–specific primers identified P. gingivalis DNA in samples. ( G ) qPCR analysis of saliva samples. The bar graphs in (F) and (G) show the means and SEMs of three replicates. ( H ) COR388 treatment of W83 culture in defined growth medium reduced growth similarly to a Kgp-deficient strain (ΔKgp) over 43 hours. ( I ) Resistance developed rapidly to moxifloxacin but not COR388 with repeat passaging of bacterial culture. ( J to L ) Efficacy of COR388 at three oral doses of 3, 10, and 30 mg/kg twice daily in treating an established P. gingivalis brain infection in mice. Reduction of brain tissue levels of P. gingivalis (J), Aβ 1–42 (K), and TNFα (L). The bar graphs show the means with SEM error bars. *** P

    Journal: Science Advances

    Article Title: Porphyromonas gingivalis in Alzheimer’s disease brains: Evidence for disease causation and treatment with small-molecule inhibitors

    doi: 10.1126/sciadv.aau3333

    Figure Lengend Snippet: COR388 target engagement and dose-dependent effects on brain P. gingivalis , Aβ 1–42 , and TNFα in mice. ( A ) COR553 fluorescent activity probe for Kgp. ( B ) COR553 labeling of Kgp in P. gingivalis W83 strain and no labeling in mutant deficient in Kgp (ΔKgp). ( C ) W83 lysates labeled with COR553. Left lane, before immunodepletion; middle lane, after immunodepletion with anti-Kgp–conjugated beads; right lane, after elution from anti-Kgp–conjugated beads. ( D ) W83 strain titrated and labeled with COR553 to determine the limit of bacterial detection. See Results for details. ( E ) Oral plaque samples from human subjects (CB1-5) with periodontal disease were incubated ex vivo with COR553 probe with or without preincubation with COR388. COR553 probe and CAB102 detected Kgp strongly in three subjects (CB1, CB4, and CB5) and weakly in one subject (CB3). COR388 preincubation blocked COR553 probe binding to Kgp. ( F ) qPCR analysis of plaque samples using hmuY gene–specific primers identified P. gingivalis DNA in samples. ( G ) qPCR analysis of saliva samples. The bar graphs in (F) and (G) show the means and SEMs of three replicates. ( H ) COR388 treatment of W83 culture in defined growth medium reduced growth similarly to a Kgp-deficient strain (ΔKgp) over 43 hours. ( I ) Resistance developed rapidly to moxifloxacin but not COR388 with repeat passaging of bacterial culture. ( J to L ) Efficacy of COR388 at three oral doses of 3, 10, and 30 mg/kg twice daily in treating an established P. gingivalis brain infection in mice. Reduction of brain tissue levels of P. gingivalis (J), Aβ 1–42 (K), and TNFα (L). The bar graphs show the means with SEM error bars. *** P

    Article Snippet: The qPCR reaction mixture contained 100 ng of brain DNA, 0.5 μM primers/0.15 μM probe, and Kapa Fast qPCR Mix (Kapa Biosystems).

    Techniques: Mouse Assay, Activity Assay, Labeling, Mutagenesis, Incubation, Ex Vivo, Binding Assay, Real-time Polymerase Chain Reaction, Passaging, Infection

    Identification of P. gingivalis –specific protein and DNA in cortex from control and AD patients. ( A ) WB with four different strains of P. gingivalis and CAB102 detection of typical molecular weight bands for Kgp in bacterial lysates. ( B ) IP using brain lysates from nondemented controls (C1 to C6; ages 75, 54, 63, 45, 37, and 102 years, respectively) and AD patients (AD1 to AD3; ages 83, 90, and 80 years, respectively) using CAB102 with subsequent WB reveals the ~50-kDa Kgp catalytic subunit (Kgp cat ), along with higher– and lower–molecular weight Kgp species seen in (A). ( C ) qPCR from DNA isolated from the same brain lysates as the protein samples analyzed in (B) shows a positive signal in nondemented control (C1 to C5) and AD (AD1 to AD3) samples. Sample C6 from the 102-year-old nondemented control patient had no detectable qPCR signal in (C) and very faint bands indicating near absence of Kgp (B) (mean with SEM error bars of repeat qPCR runs).

    Journal: Science Advances

    Article Title: Porphyromonas gingivalis in Alzheimer’s disease brains: Evidence for disease causation and treatment with small-molecule inhibitors

    doi: 10.1126/sciadv.aau3333

    Figure Lengend Snippet: Identification of P. gingivalis –specific protein and DNA in cortex from control and AD patients. ( A ) WB with four different strains of P. gingivalis and CAB102 detection of typical molecular weight bands for Kgp in bacterial lysates. ( B ) IP using brain lysates from nondemented controls (C1 to C6; ages 75, 54, 63, 45, 37, and 102 years, respectively) and AD patients (AD1 to AD3; ages 83, 90, and 80 years, respectively) using CAB102 with subsequent WB reveals the ~50-kDa Kgp catalytic subunit (Kgp cat ), along with higher– and lower–molecular weight Kgp species seen in (A). ( C ) qPCR from DNA isolated from the same brain lysates as the protein samples analyzed in (B) shows a positive signal in nondemented control (C1 to C5) and AD (AD1 to AD3) samples. Sample C6 from the 102-year-old nondemented control patient had no detectable qPCR signal in (C) and very faint bands indicating near absence of Kgp (B) (mean with SEM error bars of repeat qPCR runs).

    Article Snippet: The qPCR reaction mixture contained 100 ng of brain DNA, 0.5 μM primers/0.15 μM probe, and Kapa Fast qPCR Mix (Kapa Biosystems).

    Techniques: Western Blot, Molecular Weight, Real-time Polymerase Chain Reaction, Isolation

    Detection of P. gingivalis in CSF and oral biofluids from clinical AD subjects. ( A ) Detection and quantitation of P. gingivalis DNA by qPCR in CSF from subjects with probable AD. ( B ) Detection and quantitation of P. gingivalis DNA by qPCR from matching saliva samples. ( C ) Top: PCR products detecting P. gingivalis from CSF in (A) from all subjects run on agarose gel including negative and positive controls containing a synthetic DNA template. Faint or undetectable PCR products from subjects AD1, AD3, and AD5 were below the limit of quantitation for copy number and not of sufficient quantity for sequence analysis. Bottom: qPCR products from CSF from the same subjects for H. pylori. ( D ) Data table includes age and Mini Mental Status Exam (MMSE) score on subjects and sequence identity of PCR products to P. gingivalis hmuY DNA sequence. Sequence data are included in fig. S4. NS, not sequenced.

    Journal: Science Advances

    Article Title: Porphyromonas gingivalis in Alzheimer’s disease brains: Evidence for disease causation and treatment with small-molecule inhibitors

    doi: 10.1126/sciadv.aau3333

    Figure Lengend Snippet: Detection of P. gingivalis in CSF and oral biofluids from clinical AD subjects. ( A ) Detection and quantitation of P. gingivalis DNA by qPCR in CSF from subjects with probable AD. ( B ) Detection and quantitation of P. gingivalis DNA by qPCR from matching saliva samples. ( C ) Top: PCR products detecting P. gingivalis from CSF in (A) from all subjects run on agarose gel including negative and positive controls containing a synthetic DNA template. Faint or undetectable PCR products from subjects AD1, AD3, and AD5 were below the limit of quantitation for copy number and not of sufficient quantity for sequence analysis. Bottom: qPCR products from CSF from the same subjects for H. pylori. ( D ) Data table includes age and Mini Mental Status Exam (MMSE) score on subjects and sequence identity of PCR products to P. gingivalis hmuY DNA sequence. Sequence data are included in fig. S4. NS, not sequenced.

    Article Snippet: The qPCR reaction mixture contained 100 ng of brain DNA, 0.5 μM primers/0.15 μM probe, and Kapa Fast qPCR Mix (Kapa Biosystems).

    Techniques: Quantitation Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Sequencing

    Expression of 62 activation-related host genes in CB or AB mDC inoculated with rgRSV or IAV. CB or AB mDC ( n = 3 different CB or AB donors) were mock inoculated, inoculated with rgRSV or IAV at an MOI of 3 PFU/cell, or stimulated with 1 μg/ml of LPS. At 24 hpi, total cellular RNA was prepared and reverse transcribed using random primers. The cDNA was analyzed by qPCR using a custom-made 62-gene TaqMan array. qPCR results were analyzed using the comparative threshold cycle (ΔΔ C T ) method and normalized to the amount of 18S rRNA. The results are presented as the fold increase (red) or decrease (green) (in log 2 ) in gene expression over that for mock-inoculated cells from the same donor. Treg, regulatory T cells.

    Journal: Journal of Virology

    Article Title: Lack of Activation Marker Induction and Chemokine Receptor Switch in Human Neonatal Myeloid Dendritic Cells in Response to Human Respiratory Syncytial Virus

    doi: 10.1128/JVI.01216-19

    Figure Lengend Snippet: Expression of 62 activation-related host genes in CB or AB mDC inoculated with rgRSV or IAV. CB or AB mDC ( n = 3 different CB or AB donors) were mock inoculated, inoculated with rgRSV or IAV at an MOI of 3 PFU/cell, or stimulated with 1 μg/ml of LPS. At 24 hpi, total cellular RNA was prepared and reverse transcribed using random primers. The cDNA was analyzed by qPCR using a custom-made 62-gene TaqMan array. qPCR results were analyzed using the comparative threshold cycle (ΔΔ C T ) method and normalized to the amount of 18S rRNA. The results are presented as the fold increase (red) or decrease (green) (in log 2 ) in gene expression over that for mock-inoculated cells from the same donor. Treg, regulatory T cells.

    Article Snippet: Full-length cDNAs were produced by reverse transcription at 53°C of polyadenylated mRNA using bar-coded primers containing a 30-nt poly(T) primer sequence and pooled by demulsification. cDNA libraries were amplified by PCR and quantified during construction and for final quality control via a high-sensitivity DNA reagents kit using a 2100 Bioanalyzer (Agilent Technologies). cDNA copy numbers were determined using a Kapa library quantification kit (Illumina) universal qPCR mix (Kapa Biosystems) and a CFX96 real-time PCR system (Bio-Rad).

    Techniques: Expressing, Activation Assay, Real-time Polymerase Chain Reaction

    Effects of DEX on interaction between NeuroD1 and E-box on Pomc promoter in AtT20 cells. (A) Effect of of DEX on interaction between NeuroD1 and E-box on Pomc promoter examined by ChIP assay using E-box primer. ChIP assay was performed using digested chromatin extracted from the cells cultured in the presence (100 nM) or absence (control) of DEX for 30 min (B), 60 min (C), or 24 hrs (D). Chromatin fragments were immunoprecipitated either by normal rabbit IgG (negative control) or NeuroD1 antibody. Purified DNA was analyzed by qPCR using primers specific for E-box containing sequence on Pomc promoter. The expected size of E-box is 196 bp. Few qPCR products observed in the input samples were detected in the immunoprecipitation using normal IgG. Immunoprecipitated DNA was quantified by qPCR and normalized to the values obtained after amplification of unprecipitated 1% input DNA. Each point represents mean ± SEM (n = 3). **P

    Journal: PLoS ONE

    Article Title: Role of NeuroD1 on the negative regulation of Pomc expression by glucocorticoid

    doi: 10.1371/journal.pone.0175435

    Figure Lengend Snippet: Effects of DEX on interaction between NeuroD1 and E-box on Pomc promoter in AtT20 cells. (A) Effect of of DEX on interaction between NeuroD1 and E-box on Pomc promoter examined by ChIP assay using E-box primer. ChIP assay was performed using digested chromatin extracted from the cells cultured in the presence (100 nM) or absence (control) of DEX for 30 min (B), 60 min (C), or 24 hrs (D). Chromatin fragments were immunoprecipitated either by normal rabbit IgG (negative control) or NeuroD1 antibody. Purified DNA was analyzed by qPCR using primers specific for E-box containing sequence on Pomc promoter. The expected size of E-box is 196 bp. Few qPCR products observed in the input samples were detected in the immunoprecipitation using normal IgG. Immunoprecipitated DNA was quantified by qPCR and normalized to the values obtained after amplification of unprecipitated 1% input DNA. Each point represents mean ± SEM (n = 3). **P

    Article Snippet: Immunoprecipitated DNA was analyzed by qPCR using KAPA SYBR FAST Universal 2x qPCR Master Mix (KAPA Biosystems) together with 1% of the input chromatin.

    Techniques: Chromatin Immunoprecipitation, Cell Culture, Immunoprecipitation, Negative Control, Purification, Real-time Polymerase Chain Reaction, Sequencing, Amplification

    p30 decreases TRL3 and TRL4 but not TLR7/8 signaling. (A to C) Real-time quantitative PCR analysis of type 1 ISGs. THP-1 cells were transduced with p30 or control lentivirus, kept in culture for 72 h, and stimulated for 6 h with poly(I·C) (A),

    Journal: Journal of Virology

    Article Title: Human T-Cell Leukemia/Lymphoma Virus Type 1 p30, but Not p12/p8, Counteracts Toll-Like Receptor 3 (TLR3) and TLR4 Signaling in Human Monocytes and Dendritic Cells

    doi: 10.1128/JVI.01788-13

    Figure Lengend Snippet: p30 decreases TRL3 and TRL4 but not TLR7/8 signaling. (A to C) Real-time quantitative PCR analysis of type 1 ISGs. THP-1 cells were transduced with p30 or control lentivirus, kept in culture for 72 h, and stimulated for 6 h with poly(I·C) (A),

    Article Snippet: Reactions were performed using a SYBR fast quantitative PCR (qPCR) mix (KapaBiosystems, Woburn, MA).

    Techniques: Real-time Polymerase Chain Reaction, Transduction