polymerase chain reaction pcr master mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher polymerase chain reaction pcr master mix
    Polymerase Chain Reaction Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr master mix/product/Thermo Fisher
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr master mix - by Bioz Stars, 2020-08
    90/100 stars

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    Polymerase Chain Reaction:

    Article Title: Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused Three-Dimensional Multicompartment Bioreactor
    Article Snippet: .. Each cDNA template was mixed with polymerase chain reaction (PCR) Master mix (Applied Biosystems) and human-specific primers and probes (TaqMan Gene Expression Assay system; Life Technologies; ). .. Quantitative real-time PCR (qRT-PCR) was performed using a Real time cycler (Mastercycler ep Realplex 2; Eppendorf).

    Article Title: The anti-inflammatory effect and potential mechanism of cardamonin in DSS-induced colitis
    Article Snippet: .. Polymerase chain reaction (PCR) Master Mix (2×) was from Thermo Scientific (Waltham, MA). .. Antibodies for inducible nitric oxide (NO) synthase (iNOS; no. 13120), phosphorylated inhibitor κB kinase (p-IKK)-α/β (no. 2697); NF-κB p65 (no. 8242), p-p65 (no. 3033), inhibitor κBα (IκBα) (no. 4814), p-IκBα (no. 2859), extracellular signal-regulated kinase (ERK) 1/2 (no. 4348), p-ERK1/2 (no. 4377), c-Jun NH2 -terminal kinase (JNK) (no. 9255), p-JNK (no. 9252), and β-actin (no. 4970) were obtained from Cell Signaling Technology (Danvers, MA).

    Article Title: Inhibition of cAMP/PKA Pathway Protects Optic Nerve Head Astrocytes against Oxidative Stress by Akt/Bax Phosphorylation-Mediated Mfn1/2 Oligomerization
    Article Snippet: .. Reagents and Other Materials Chemicals and other materials were obtained from the following sources: hydrogen peroxide (H2 O2 ), dibutyryl-cAMP (dbcAMP), and H89 from Sigma, St. Louis, MO, USA; 8CPT-6phenyl-cAMP from Biolog; DMEM/F12 (1 : 1), MEM+GlutaMax™, 100x penicillin/streptomycin (Pen/Strep), and fetal bovine serum from Thermo Fisher Scientific, Waltham, MA, USA; Superscript™ II reverse transcriptase from Invitrogen; RNase inhibitor, dNTP mixture, and oligo (dT) from Promega; polymerase chain reaction (PCR) master mix, protein size marker, and SuperSignal® West Pico and Femto chemiluminescent substrates from Thermo Fisher Scientific; and Amersham Hybond™-P polyvinylidenedifluoride (PVDF) membrane and ECL™ Prime Western Blotting Detection Reagent from GE Healthcare (Chicago, IL, USA). .. Tissue Preparation Mice were anesthetized with intraperitoneal injection of a mixture of ketamine (100 mg/kg, Ketaset; Fort Dodge Animal Health, Fort Dodge, IA, USA) and xylazine (9 mg/kg, TranquiVed; Vedco, Inc., St. Joseph, MO, USA) before cervical dislocation.

    Article Title: Administration of polysaccharides from Antrodia camphorata modulates dendritic cell function and alleviates allergen-induced T helper type 2 responses in a mouse model of asthma
    Article Snippet: .. Four microlitres of cDNA was used with a polymerase chain reaction (PCR) master mix and Taq Man assays (Applied Biosystems). .. Quantitative PCR detection of mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH), IL-10 and IL-12 were conducted in triplicate using an Applied Biosystems 7900 PCR system.

    Article Title: Recombinant Lactococcus lactis Expressing Ling Zhi 8 Protein Ameliorates Nonalcoholic Fatty Liver and Early Atherogenesis in Cholesterol-Fed Rabbits
    Article Snippet: .. A total volume of 20 μ L of PCR mixture included 10 μ l of polymerase chain reaction (PCR) Master Mix (Applied Biosystems, Life Technologies, Carlsbad, CA, USA), 10 pmoles of specific sense and antisense primers for each cytokine gene, and 10 ng of first-strand cDNA. .. After beginning with a single preincubation step at 95°C for 10 min, PCR was performed under the following conditions: denaturation for 1 min at 94°C, annealing for 1 min at 55°C, and extension for 1 min at 72°C in a thermal cycler (G-STORM, UK).

    Marker:

    Article Title: Inhibition of cAMP/PKA Pathway Protects Optic Nerve Head Astrocytes against Oxidative Stress by Akt/Bax Phosphorylation-Mediated Mfn1/2 Oligomerization
    Article Snippet: .. Reagents and Other Materials Chemicals and other materials were obtained from the following sources: hydrogen peroxide (H2 O2 ), dibutyryl-cAMP (dbcAMP), and H89 from Sigma, St. Louis, MO, USA; 8CPT-6phenyl-cAMP from Biolog; DMEM/F12 (1 : 1), MEM+GlutaMax™, 100x penicillin/streptomycin (Pen/Strep), and fetal bovine serum from Thermo Fisher Scientific, Waltham, MA, USA; Superscript™ II reverse transcriptase from Invitrogen; RNase inhibitor, dNTP mixture, and oligo (dT) from Promega; polymerase chain reaction (PCR) master mix, protein size marker, and SuperSignal® West Pico and Femto chemiluminescent substrates from Thermo Fisher Scientific; and Amersham Hybond™-P polyvinylidenedifluoride (PVDF) membrane and ECL™ Prime Western Blotting Detection Reagent from GE Healthcare (Chicago, IL, USA). .. Tissue Preparation Mice were anesthetized with intraperitoneal injection of a mixture of ketamine (100 mg/kg, Ketaset; Fort Dodge Animal Health, Fort Dodge, IA, USA) and xylazine (9 mg/kg, TranquiVed; Vedco, Inc., St. Joseph, MO, USA) before cervical dislocation.

    Expressing:

    Article Title: Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused Three-Dimensional Multicompartment Bioreactor
    Article Snippet: .. Each cDNA template was mixed with polymerase chain reaction (PCR) Master mix (Applied Biosystems) and human-specific primers and probes (TaqMan Gene Expression Assay system; Life Technologies; ). .. Quantitative real-time PCR (qRT-PCR) was performed using a Real time cycler (Mastercycler ep Realplex 2; Eppendorf).

    Western Blot:

    Article Title: Inhibition of cAMP/PKA Pathway Protects Optic Nerve Head Astrocytes against Oxidative Stress by Akt/Bax Phosphorylation-Mediated Mfn1/2 Oligomerization
    Article Snippet: .. Reagents and Other Materials Chemicals and other materials were obtained from the following sources: hydrogen peroxide (H2 O2 ), dibutyryl-cAMP (dbcAMP), and H89 from Sigma, St. Louis, MO, USA; 8CPT-6phenyl-cAMP from Biolog; DMEM/F12 (1 : 1), MEM+GlutaMax™, 100x penicillin/streptomycin (Pen/Strep), and fetal bovine serum from Thermo Fisher Scientific, Waltham, MA, USA; Superscript™ II reverse transcriptase from Invitrogen; RNase inhibitor, dNTP mixture, and oligo (dT) from Promega; polymerase chain reaction (PCR) master mix, protein size marker, and SuperSignal® West Pico and Femto chemiluminescent substrates from Thermo Fisher Scientific; and Amersham Hybond™-P polyvinylidenedifluoride (PVDF) membrane and ECL™ Prime Western Blotting Detection Reagent from GE Healthcare (Chicago, IL, USA). .. Tissue Preparation Mice were anesthetized with intraperitoneal injection of a mixture of ketamine (100 mg/kg, Ketaset; Fort Dodge Animal Health, Fort Dodge, IA, USA) and xylazine (9 mg/kg, TranquiVed; Vedco, Inc., St. Joseph, MO, USA) before cervical dislocation.

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    Thermo Fisher nacl
    The hyphal growth defects of the Δ cnaA or Δ cnaB strains could be suppressed by the dysfunction of CchA under salt stress. (A) Colony morphology of the Δ cnaA , Δ cnaA cchA re , Δ cnaB , and Δ cnaB Δ cchA strains and the reference strain. The conidia were spotted on solid <t>MMPDRUU</t> and MMPDRUU supplemented with 800 mM <t>NaCl,</t> 600 mM KCl, or 1 M sorbitol, respectively, at 37°C for 2.5 days. (B) Graphic representation of radial growth rates of the Δ cnaA and Δ cnaA cchA re strains and the reference strain. The values are means ± standard deviations (SD) from three independent experiments. (C) Differential interference contrast images of hyphae grown on MMPDRUU in the presence or absence of salt stress at 37°C for 16 h. Bar, 10 μm. (D) Analysis of branching frequencies in hyphal filaments of the Δ cnaA and Δ cnaA cchA re strains and the reference strain. The values are means ± SD from three independent experiments.
    Nacl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nacl/product/Thermo Fisher
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    nacl - by Bioz Stars, 2020-08
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    99
    Thermo Fisher fast sybr green master mix
    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with <t>SYBR</t> ™ Safe DNA Gel Stain in agarose gels. (C) <t>qRT-PCR</t> was performed to confirm the results in (B).
    Fast Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast sybr green master mix/product/Thermo Fisher
    Average 99 stars, based on 3358 article reviews
    Price from $9.99 to $1999.99
    fast sybr green master mix - by Bioz Stars, 2020-08
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    99
    Thermo Fisher taqman master mix
    Inflammatory cytokine and Wnt mRNA levels in primary human 3D lung aggregate cultures and human macrophages (M) after cigarette smoke extracts (CSE) exposure. (A) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and CSE exposed (48 h) lung aggregate cultures, containing and not containing M. (B) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and rWnt5a (1 µg/ml) treated lung aggregate cultures, containing and not containing M. (C) <t>Taqman</t> array heat map analysis of pooled ( n = 4) <t>cDNA</t> of human M compared with control. M were treated with CSE for 3 h. (D) Changes in mRNA levels of Wnt signaling pathway genes measured by Taqman Array analysis using pooled ( n = 4) cDNA samples of human M compared with control.
    Taqman Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman master mix/product/Thermo Fisher
    Average 99 stars, based on 445 article reviews
    Price from $9.99 to $1999.99
    taqman master mix - by Bioz Stars, 2020-08
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    Image Search Results


    The hyphal growth defects of the Δ cnaA or Δ cnaB strains could be suppressed by the dysfunction of CchA under salt stress. (A) Colony morphology of the Δ cnaA , Δ cnaA cchA re , Δ cnaB , and Δ cnaB Δ cchA strains and the reference strain. The conidia were spotted on solid MMPDRUU and MMPDRUU supplemented with 800 mM NaCl, 600 mM KCl, or 1 M sorbitol, respectively, at 37°C for 2.5 days. (B) Graphic representation of radial growth rates of the Δ cnaA and Δ cnaA cchA re strains and the reference strain. The values are means ± standard deviations (SD) from three independent experiments. (C) Differential interference contrast images of hyphae grown on MMPDRUU in the presence or absence of salt stress at 37°C for 16 h. Bar, 10 μm. (D) Analysis of branching frequencies in hyphal filaments of the Δ cnaA and Δ cnaA cchA re strains and the reference strain. The values are means ± SD from three independent experiments.

    Journal: Applied and Environmental Microbiology

    Article Title: Calcineurin and Calcium Channel CchA Coordinate the Salt Stress Response by Regulating Cytoplasmic Ca2+ Homeostasis in Aspergillus nidulans

    doi: 10.1128/AEM.00330-16

    Figure Lengend Snippet: The hyphal growth defects of the Δ cnaA or Δ cnaB strains could be suppressed by the dysfunction of CchA under salt stress. (A) Colony morphology of the Δ cnaA , Δ cnaA cchA re , Δ cnaB , and Δ cnaB Δ cchA strains and the reference strain. The conidia were spotted on solid MMPDRUU and MMPDRUU supplemented with 800 mM NaCl, 600 mM KCl, or 1 M sorbitol, respectively, at 37°C for 2.5 days. (B) Graphic representation of radial growth rates of the Δ cnaA and Δ cnaA cchA re strains and the reference strain. The values are means ± standard deviations (SD) from three independent experiments. (C) Differential interference contrast images of hyphae grown on MMPDRUU in the presence or absence of salt stress at 37°C for 16 h. Bar, 10 μm. (D) Analysis of branching frequencies in hyphal filaments of the Δ cnaA and Δ cnaA cchA re strains and the reference strain. The values are means ± SD from three independent experiments.

    Article Snippet: A total of 1 × 106 spores were cultured on either MMPDRUU or MMPDRUU with 800 mM NaCl in a 96-well microtiter plate (Thermo Fischer, United Kingdom) and then incubated at 37°C for 18 h. The medium was then removed, and the mycelia were rinsed twice with PGM: 20 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)] (pH 6.7), 50 mM glucose, and 1 mM MgCl2 .

    Techniques:

    Colony morphologies of the Δ yvcA , Δ cnaA , and Δ cnaA Δ yvcA strains grown on MMPDRUU in the presence or absence of 800 mM NaCl or 800 mM NaCl plus 3 mM EGTA at 37°C for 2.5 days.

    Journal: Applied and Environmental Microbiology

    Article Title: Calcineurin and Calcium Channel CchA Coordinate the Salt Stress Response by Regulating Cytoplasmic Ca2+ Homeostasis in Aspergillus nidulans

    doi: 10.1128/AEM.00330-16

    Figure Lengend Snippet: Colony morphologies of the Δ yvcA , Δ cnaA , and Δ cnaA Δ yvcA strains grown on MMPDRUU in the presence or absence of 800 mM NaCl or 800 mM NaCl plus 3 mM EGTA at 37°C for 2.5 days.

    Article Snippet: A total of 1 × 106 spores were cultured on either MMPDRUU or MMPDRUU with 800 mM NaCl in a 96-well microtiter plate (Thermo Fischer, United Kingdom) and then incubated at 37°C for 18 h. The medium was then removed, and the mycelia were rinsed twice with PGM: 20 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)] (pH 6.7), 50 mM glucose, and 1 mM MgCl2 .

    Techniques:

    Expression analysis of Ca 2+ -signaling-related and salt-stress-induced genes in response to salt stress by quantitative PCR. (A) Fold changes in mRNA levels, including vcxA , yvcA , pmrA , and pmcA , after incubation with MMPDRUU with addition of 800 mM NaCl compared to results with MMPDRUU alone using real-time RT-PCR. (B) Fold changes in mRNA levels, including enaA , nhaA , and trkA , after incubation with MMPDRUU with addition of 800 mM NaCl compared to results with MMPDRUU alone using real-time RT-PCR. Data representing the indicated strains' mRNA levels from salt stress pretreatment were normalized to the non-salt-pretreatment condition. The error bars indicate the standard deviations from three independent replicates.

    Journal: Applied and Environmental Microbiology

    Article Title: Calcineurin and Calcium Channel CchA Coordinate the Salt Stress Response by Regulating Cytoplasmic Ca2+ Homeostasis in Aspergillus nidulans

    doi: 10.1128/AEM.00330-16

    Figure Lengend Snippet: Expression analysis of Ca 2+ -signaling-related and salt-stress-induced genes in response to salt stress by quantitative PCR. (A) Fold changes in mRNA levels, including vcxA , yvcA , pmrA , and pmcA , after incubation with MMPDRUU with addition of 800 mM NaCl compared to results with MMPDRUU alone using real-time RT-PCR. (B) Fold changes in mRNA levels, including enaA , nhaA , and trkA , after incubation with MMPDRUU with addition of 800 mM NaCl compared to results with MMPDRUU alone using real-time RT-PCR. Data representing the indicated strains' mRNA levels from salt stress pretreatment were normalized to the non-salt-pretreatment condition. The error bars indicate the standard deviations from three independent replicates.

    Article Snippet: A total of 1 × 106 spores were cultured on either MMPDRUU or MMPDRUU with 800 mM NaCl in a 96-well microtiter plate (Thermo Fischer, United Kingdom) and then incubated at 37°C for 18 h. The medium was then removed, and the mycelia were rinsed twice with PGM: 20 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)] (pH 6.7), 50 mM glucose, and 1 mM MgCl2 .

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Incubation, Quantitative RT-PCR

    Relative expression of the SND1 transcript in plants subjected to different hormone treatments or abiotic stresses. The relative expression of SND1 transcript in Col-0 was determined by the qRT-PCR. Eight-day-old seedlings were used to extract mRNA following the treatment with 10 µM abscisic acid (ABA), 10 µM indole-3-acetic acid (IAA), 10 µM jasmonic acid (JA), 10 µM salicylic acid (SA), 300 mM sucrose (Suc), 400 mM mannitol (Man), and 300 mM NaCl for 6 h. The error bars indicate the standard error (SE) of three replicates. The values with different letters were significantly different from that of WT plants (P

    Journal: Scientific Reports

    Article Title: Dual role of SND1 facilitates efficient communication between abiotic stress signalling and normal growth in Arabidopsis

    doi: 10.1038/s41598-018-28413-x

    Figure Lengend Snippet: Relative expression of the SND1 transcript in plants subjected to different hormone treatments or abiotic stresses. The relative expression of SND1 transcript in Col-0 was determined by the qRT-PCR. Eight-day-old seedlings were used to extract mRNA following the treatment with 10 µM abscisic acid (ABA), 10 µM indole-3-acetic acid (IAA), 10 µM jasmonic acid (JA), 10 µM salicylic acid (SA), 300 mM sucrose (Suc), 400 mM mannitol (Man), and 300 mM NaCl for 6 h. The error bars indicate the standard error (SE) of three replicates. The values with different letters were significantly different from that of WT plants (P

    Article Snippet: RNA isolation and quantitative real-time RT-PCR The total RNA was isolated from eight-day-old seedlings following treatment with 10 µM ABA, 10 µM Indole-3-acetic acid (IAA), 10 µM Jasmonic acid (JA), 10 µM Salicylic acid (SA), 200 mM NaCl, 300 mM NaCl, 400 mM mannitol, or 200 mM sucrose for 6 h. The cDNA was synthesized using the total mRNA samples from eight-day-old seedlings and a cDNA synthesis kit (RevertAid First Strand cDNA Synthesis Kit, ThermoScientific).

    Techniques: Expressing, Quantitative RT-PCR

    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Journal: Heart rhythm

    Article Title: HuR-mediated SCN5A mRNA stability reduces arrhythmic risk in heart failure

    doi: 10.1016/j.hrthm.2018.02.018

    Figure Lengend Snippet: HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Article Snippet: Quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) was carried out using gene-specific primers, Fast SYBR® Green Master Mix and 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Isolation, Synthesized, Polymerase Chain Reaction, Staining, Quantitative RT-PCR

    Inflammatory cytokine and Wnt mRNA levels in primary human 3D lung aggregate cultures and human macrophages (M) after cigarette smoke extracts (CSE) exposure. (A) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and CSE exposed (48 h) lung aggregate cultures, containing and not containing M. (B) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and rWnt5a (1 µg/ml) treated lung aggregate cultures, containing and not containing M. (C) Taqman array heat map analysis of pooled ( n = 4) cDNA of human M compared with control. M were treated with CSE for 3 h. (D) Changes in mRNA levels of Wnt signaling pathway genes measured by Taqman Array analysis using pooled ( n = 4) cDNA samples of human M compared with control.

    Journal: Frontiers in Immunology

    Article Title: Cigarette Smoke-Induced Pulmonary Inflammation Becomes Systemic by Circulating Extracellular Vesicles Containing Wnt5a and Inflammatory Cytokines

    doi: 10.3389/fimmu.2018.01724

    Figure Lengend Snippet: Inflammatory cytokine and Wnt mRNA levels in primary human 3D lung aggregate cultures and human macrophages (M) after cigarette smoke extracts (CSE) exposure. (A) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and CSE exposed (48 h) lung aggregate cultures, containing and not containing M. (B) IL-6 and IL-8 inflammatory cytokine mRNA levels in control and rWnt5a (1 µg/ml) treated lung aggregate cultures, containing and not containing M. (C) Taqman array heat map analysis of pooled ( n = 4) cDNA of human M compared with control. M were treated with CSE for 3 h. (D) Changes in mRNA levels of Wnt signaling pathway genes measured by Taqman Array analysis using pooled ( n = 4) cDNA samples of human M compared with control.

    Article Snippet: TaqMan master mix was combined with the cDNA samples then the mixed solutions were loaded onto the Human WNT Pathway, Fast 96-well TaqMan Array (Thermo Fisher Scientific, Waltham, MA, USA) plate.

    Techniques: