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TaKaRa polymerase chain reaction pcr master mix
Polymerase Chain Reaction Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr master mix/product/TaKaRa
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr master mix - by Bioz Stars, 2020-08
88/100 stars

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Polymerase Chain Reaction:

Article Title: Evaluation of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method for assessing biofilm formation in vitro by Trichosporon spp.
Article Snippet: .. [ ] The polymerase chain reaction (PCR) master mix was prepared containing 25 μl of PCR mix (Takara, Japan), 1 μl of forward (TRF) and reverse primer (TRR) (GeNei, Bengaluru), 1 μl of template DNA, and the volume made up to 50 μl with sterile nuclease-free water. .. The reaction mixtures were amplified in a thermal cycler (Veriti 96 well, Applied Biosystems, USA), with the following program: 95°C for 7 min, followed by 30 cycles consisting of 95°C for 30 s, 54°C for 30 s, and 72°C for 30 s, with a final extension period at 72°C for 10 min.[ ] After thermal cycling, 10 μl of the amplified product was run on a 1.5% (wt/vol) agarose gel, stained with ethidium bromide, and visualized with ultraviolet light.

Article Title: Small ubiquitin-related modifier 2/3 interacts with p65 and stabilizes it in the cytoplasm in HBV-associated hepatocellular carcinoma
Article Snippet: .. Polymerase chain reaction (PCR) Master Mix was obtained from TaKaRa Biotechnology (Dalian, China). ..

Article Title: Inhibition of 5-Lipoxygenase Pathway Attenuates Acute Liver Failure by Inhibiting Macrophage Activation
Article Snippet: .. The SuperScript II Reverse Transcriptase Kit, TaqMan Gene Expression Assay, and universal TaqMan 2x polymerase chain reaction (PCR) master mix were from Takara Bio (Dalian, China). .. Animal Model Seventy-six male Wistar rats were randomly divided into three groups. (1) Control group (6 rats): DMSO (1.5 mL) was injected intraperitoneally.

Expressing:

Article Title: Inhibition of 5-Lipoxygenase Pathway Attenuates Acute Liver Failure by Inhibiting Macrophage Activation
Article Snippet: .. The SuperScript II Reverse Transcriptase Kit, TaqMan Gene Expression Assay, and universal TaqMan 2x polymerase chain reaction (PCR) master mix were from Takara Bio (Dalian, China). .. Animal Model Seventy-six male Wistar rats were randomly divided into three groups. (1) Control group (6 rats): DMSO (1.5 mL) was injected intraperitoneally.

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  • 93
    TaKaRa tb green pcr master mix
    A , Quantitative <t>PCR</t> (qPCR) analysis of DONSON mRNA amount in HeLa cells. Total RNA was isolated from HeLa cells after 48 h treatment of the indicated siRNAs, and was subjected to reverse transcription. The resulting <t>cDNA</t> was subjected to real-time qPCR analysis. B, Time-lapse observation of DONSON localization at different cell cycle stages. HeLa GFP-DONSON cells were visualized every 10 min for 24 h after 24 h treatment of 1 μg/mL Doxycycline and 3 h treatment of 100 nM SiR-DNA. DNA (blue) and GFP-DONSON (green). Arrowheads indicate GFP-DONSON foci. C, The centrosomal localization of DONSON in mitosis. HeLa GFP-DONSON cells were fixed after 24 h treatment of 1 μg/mL Doxycycline and were immunostained with antibodies against GFP (green) and CP110 (red). D, Phenotype rescue experiments by expression of RNAi-resistant (RNAi-R) form of full-length (FL) or nuclear localization signal deleted (ΔNLS) DONSON. HeLa cells were treated with siControl or siDONSON, followed by transfection with FLAG empty (control), RNAi-resistant DONSON-FL, or DONSON-ΔNLS. The cells were immunostained with antibodies against FLAG (green) and Cep192 (red). Arrowheads indicate Cep192 foci. E, Histograms represent frequency of interphase cells with the indicated phenotypes observed in D. Values are mean percentages ± s.d. from three independent experiments (n = 30 for each experiment). Tukey’s multiple comparisons test was used in E to obtain P value. **, p
    Tb Green Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tb green pcr master mix/product/TaKaRa
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tb green pcr master mix - by Bioz Stars, 2020-08
    93/100 stars
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    89
    TaKaRa sybr green qrt pcr master mix
    PTEN is a direct target gene of miR-130b. ( A ) PTEN was detected by <t>qRT-</t> <t>PCR</t> in 29 BC tissues and was matched the adjacent noncancerous breast tissues. ( B ) The relationship between PTEN and miR-130b expression was explored by Spearman’s correlation in 29 paired BC tissues and adjacent noncancerous breast tissues. PTEN was inversely correlated with the miR-130b levels in BC tissues. ( C ) Relative PTEN expression in MCF-7 and MCF-7/ADR cells were detected by qRT-PCR (left panel) and western blot (right panel). ( D ) Effects of miR-130b mimics in MCF-7 cells and miR-130b inhibitor in MCF-7/ADR cells on the relative expression levels of PTEN were detected by qRT- PCR (left panel) and western blot (right panel). ( E ) PTEN expression was detected by immunofluorescence staining in these cells. Red fluorescence: PTEN, blue fluorescence: DAPI, staining for nuclear DNA. There were no significant differences between the control group and the blank group. ( F ) The relative luciferase activity of 293 T cells was detected after the pGL3-control vector, wt or mut PTEN 3′UTR genes were co-transfected with miR-NC or miR-130b mimics (*P
    Sybr Green Qrt Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green qrt pcr master mix/product/TaKaRa
    Average 89 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    sybr green qrt pcr master mix - by Bioz Stars, 2020-08
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    94
    TaKaRa pcr master mix
    Alternative PNRC2 transcripts caused by a chimpanzee-specific ERV. (A) Using the UCSC Genome Browser, the PNRC2 gene is anatomized into intron, un-translated region (UTR), and coding exon. (B) The structures of chimpanzee and non-chimpanzee primate PNRC2 s and their transcripts are depicted. The black and gray boxes indicate an exon and UTRs, respectively, and the red box denotes the untranscribed UTR1 of the chimpanzee PNRC2 . Four different primers used to amplify <t>PCR</t> products are shown in red, blue, green, and black arrows, respectively. The target site duplications (TSDs) of the full-length chimpanzee-specific ERV are written in red. (C) The picture shows an agarose gel image of PCR products. Genomic DNAs from four different primates (human, chimpanzee, gorilla, and orangutan) were used as a <t>DNA</t> template for each PCR reaction.
    Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/TaKaRa
    Average 94 stars, based on 128 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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    A , Quantitative PCR (qPCR) analysis of DONSON mRNA amount in HeLa cells. Total RNA was isolated from HeLa cells after 48 h treatment of the indicated siRNAs, and was subjected to reverse transcription. The resulting cDNA was subjected to real-time qPCR analysis. B, Time-lapse observation of DONSON localization at different cell cycle stages. HeLa GFP-DONSON cells were visualized every 10 min for 24 h after 24 h treatment of 1 μg/mL Doxycycline and 3 h treatment of 100 nM SiR-DNA. DNA (blue) and GFP-DONSON (green). Arrowheads indicate GFP-DONSON foci. C, The centrosomal localization of DONSON in mitosis. HeLa GFP-DONSON cells were fixed after 24 h treatment of 1 μg/mL Doxycycline and were immunostained with antibodies against GFP (green) and CP110 (red). D, Phenotype rescue experiments by expression of RNAi-resistant (RNAi-R) form of full-length (FL) or nuclear localization signal deleted (ΔNLS) DONSON. HeLa cells were treated with siControl or siDONSON, followed by transfection with FLAG empty (control), RNAi-resistant DONSON-FL, or DONSON-ΔNLS. The cells were immunostained with antibodies against FLAG (green) and Cep192 (red). Arrowheads indicate Cep192 foci. E, Histograms represent frequency of interphase cells with the indicated phenotypes observed in D. Values are mean percentages ± s.d. from three independent experiments (n = 30 for each experiment). Tukey’s multiple comparisons test was used in E to obtain P value. **, p

    Journal: bioRxiv

    Article Title: DONSON, a gene responsible for microcephalic primordial dwarfism, ensures proper centriole duplication cycle by maintaining centriole engagement during interphase

    doi: 10.1101/2020.05.10.086777

    Figure Lengend Snippet: A , Quantitative PCR (qPCR) analysis of DONSON mRNA amount in HeLa cells. Total RNA was isolated from HeLa cells after 48 h treatment of the indicated siRNAs, and was subjected to reverse transcription. The resulting cDNA was subjected to real-time qPCR analysis. B, Time-lapse observation of DONSON localization at different cell cycle stages. HeLa GFP-DONSON cells were visualized every 10 min for 24 h after 24 h treatment of 1 μg/mL Doxycycline and 3 h treatment of 100 nM SiR-DNA. DNA (blue) and GFP-DONSON (green). Arrowheads indicate GFP-DONSON foci. C, The centrosomal localization of DONSON in mitosis. HeLa GFP-DONSON cells were fixed after 24 h treatment of 1 μg/mL Doxycycline and were immunostained with antibodies against GFP (green) and CP110 (red). D, Phenotype rescue experiments by expression of RNAi-resistant (RNAi-R) form of full-length (FL) or nuclear localization signal deleted (ΔNLS) DONSON. HeLa cells were treated with siControl or siDONSON, followed by transfection with FLAG empty (control), RNAi-resistant DONSON-FL, or DONSON-ΔNLS. The cells were immunostained with antibodies against FLAG (green) and Cep192 (red). Arrowheads indicate Cep192 foci. E, Histograms represent frequency of interphase cells with the indicated phenotypes observed in D. Values are mean percentages ± s.d. from three independent experiments (n = 30 for each experiment). Tukey’s multiple comparisons test was used in E to obtain P value. **, p

    Article Snippet: Reverse transcription and real-time qPCR Total RNA (1 μg) isolated from cells with the use of TRIZOL reagent (Thermo Fischer Scientific) was subject to reverse transcription using Quantiscript Reverse Transcription Kit (QIAGEN), and the resulting cDNA was subjected to real-time qPCR analysis with TB Green PCR Master Mix (TaKaRa) and specific primers in a StepOnePlus Real-Time PCR system (Applied Biosystems).

    Techniques: Real-time Polymerase Chain Reaction, Isolation, Expressing, Transfection

    PTEN is a direct target gene of miR-130b. ( A ) PTEN was detected by qRT- PCR in 29 BC tissues and was matched the adjacent noncancerous breast tissues. ( B ) The relationship between PTEN and miR-130b expression was explored by Spearman’s correlation in 29 paired BC tissues and adjacent noncancerous breast tissues. PTEN was inversely correlated with the miR-130b levels in BC tissues. ( C ) Relative PTEN expression in MCF-7 and MCF-7/ADR cells were detected by qRT-PCR (left panel) and western blot (right panel). ( D ) Effects of miR-130b mimics in MCF-7 cells and miR-130b inhibitor in MCF-7/ADR cells on the relative expression levels of PTEN were detected by qRT- PCR (left panel) and western blot (right panel). ( E ) PTEN expression was detected by immunofluorescence staining in these cells. Red fluorescence: PTEN, blue fluorescence: DAPI, staining for nuclear DNA. There were no significant differences between the control group and the blank group. ( F ) The relative luciferase activity of 293 T cells was detected after the pGL3-control vector, wt or mut PTEN 3′UTR genes were co-transfected with miR-NC or miR-130b mimics (*P

    Journal: Scientific Reports

    Article Title: MicroRNA-130b targets PTEN to mediate drug resistance and proliferation of breast cancer cells via the PI3K/Akt signaling pathway

    doi: 10.1038/srep41942

    Figure Lengend Snippet: PTEN is a direct target gene of miR-130b. ( A ) PTEN was detected by qRT- PCR in 29 BC tissues and was matched the adjacent noncancerous breast tissues. ( B ) The relationship between PTEN and miR-130b expression was explored by Spearman’s correlation in 29 paired BC tissues and adjacent noncancerous breast tissues. PTEN was inversely correlated with the miR-130b levels in BC tissues. ( C ) Relative PTEN expression in MCF-7 and MCF-7/ADR cells were detected by qRT-PCR (left panel) and western blot (right panel). ( D ) Effects of miR-130b mimics in MCF-7 cells and miR-130b inhibitor in MCF-7/ADR cells on the relative expression levels of PTEN were detected by qRT- PCR (left panel) and western blot (right panel). ( E ) PTEN expression was detected by immunofluorescence staining in these cells. Red fluorescence: PTEN, blue fluorescence: DAPI, staining for nuclear DNA. There were no significant differences between the control group and the blank group. ( F ) The relative luciferase activity of 293 T cells was detected after the pGL3-control vector, wt or mut PTEN 3′UTR genes were co-transfected with miR-NC or miR-130b mimics (*P

    Article Snippet: The qRT-PCR for the analysis of PTEN mRNA expression was performed using the SYBR Green qRT-PCR master mix (TaKaRa, Otsu, Shiga, Japan) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) as an internal control.

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunofluorescence, Staining, Fluorescence, Luciferase, Activity Assay, Plasmid Preparation, Transfection

    Chondrocyte marker gene expression analyses of the single subclone derived from mixed piCLCs (referred to as piCLCs-S). a Cell morphologies of piCLCs-S. b Toluidine blue staining and alcian blue staining of piCLCs-S. c, d qRT-PCR analysis of chondrocyte ( c ) and fibroblast ( d ) marker gene expression in the indicated cells. In ( c ) and ( d ), error bars indicate mean ± SD ( n = 3). e Immunofluorescence analysis showing protein expression of type I collagen (type I C), type II collagen (type II C) and aggrecan in the indicated cells. Nuclei were counterstained with DAPI

    Journal: Cell Death Discovery

    Article Title: Direct conversion of pig fibroblasts to chondrocyte-like cells by c-Myc

    doi: 10.1038/s41420-018-0136-4

    Figure Lengend Snippet: Chondrocyte marker gene expression analyses of the single subclone derived from mixed piCLCs (referred to as piCLCs-S). a Cell morphologies of piCLCs-S. b Toluidine blue staining and alcian blue staining of piCLCs-S. c, d qRT-PCR analysis of chondrocyte ( c ) and fibroblast ( d ) marker gene expression in the indicated cells. In ( c ) and ( d ), error bars indicate mean ± SD ( n = 3). e Immunofluorescence analysis showing protein expression of type I collagen (type I C), type II collagen (type II C) and aggrecan in the indicated cells. Nuclei were counterstained with DAPI

    Article Snippet: Total RNA was reversely transcribed with the PrimeScript RT reagent Kit (TaKaRa). qRT-PCR for the expression of mRNA analysis was performed using SYBR Green qRT-PCR master mix (TaKaRa) on a Stratagene Mx3005P qRT-PCR System (Agilent Technologies, Santa Clara, CA).

    Techniques: Marker, Expressing, Derivative Assay, Staining, Quantitative RT-PCR, Immunofluorescence

    Chondrocyte marker gene expression analyses of pig induced chondrocyte-like cells (piCLCs) from PEFs by c-Myc. a Cell morphologies of PEFs, pPr Ch and piCLCs. The phase-contrast photographs of c-Myc-expressing PEFs (i.e., piCLCs) and vector-expressing PEFs were token at day 4 post-infection. pPr Ch: porcine primary chondrocytes. b Toluidine blue staining and alcian blue staining for piCLCs. Proteoglycan was identified by toluidine blue staining and alcian blue staining. c , d qRT-PCR analysis of chondrocyte ( c ) and fibroblast ( d ) marker gene expression in the indicated cells. In ( c ) and ( d ), error bars indicate mean ± SD ( n = 3). e Cell extracts from pPr Ch, piCLCs and PEFs were analysed by immunoblotting with antibodies against the indicated proteins. Lane 1: pPr Ch; Lane 2: piCLCs P7; Lane 3: piCLCs P14; Lane 4: piCLCs P19; Lane 5: piCLCs P25; Lane 6: piCLCs-S; Lane 7: PEFs. f Immunofluorescence analysis showing protein expression of type I collagen (type I C), type II collagen (type II C) and aggrecan in the indicated cells. Nuclei were counterstained with DAPI

    Journal: Cell Death Discovery

    Article Title: Direct conversion of pig fibroblasts to chondrocyte-like cells by c-Myc

    doi: 10.1038/s41420-018-0136-4

    Figure Lengend Snippet: Chondrocyte marker gene expression analyses of pig induced chondrocyte-like cells (piCLCs) from PEFs by c-Myc. a Cell morphologies of PEFs, pPr Ch and piCLCs. The phase-contrast photographs of c-Myc-expressing PEFs (i.e., piCLCs) and vector-expressing PEFs were token at day 4 post-infection. pPr Ch: porcine primary chondrocytes. b Toluidine blue staining and alcian blue staining for piCLCs. Proteoglycan was identified by toluidine blue staining and alcian blue staining. c , d qRT-PCR analysis of chondrocyte ( c ) and fibroblast ( d ) marker gene expression in the indicated cells. In ( c ) and ( d ), error bars indicate mean ± SD ( n = 3). e Cell extracts from pPr Ch, piCLCs and PEFs were analysed by immunoblotting with antibodies against the indicated proteins. Lane 1: pPr Ch; Lane 2: piCLCs P7; Lane 3: piCLCs P14; Lane 4: piCLCs P19; Lane 5: piCLCs P25; Lane 6: piCLCs-S; Lane 7: PEFs. f Immunofluorescence analysis showing protein expression of type I collagen (type I C), type II collagen (type II C) and aggrecan in the indicated cells. Nuclei were counterstained with DAPI

    Article Snippet: Total RNA was reversely transcribed with the PrimeScript RT reagent Kit (TaKaRa). qRT-PCR for the expression of mRNA analysis was performed using SYBR Green qRT-PCR master mix (TaKaRa) on a Stratagene Mx3005P qRT-PCR System (Agilent Technologies, Santa Clara, CA).

    Techniques: Marker, Expressing, Plasmid Preparation, Infection, Staining, Quantitative RT-PCR, Immunofluorescence

    Alternative PNRC2 transcripts caused by a chimpanzee-specific ERV. (A) Using the UCSC Genome Browser, the PNRC2 gene is anatomized into intron, un-translated region (UTR), and coding exon. (B) The structures of chimpanzee and non-chimpanzee primate PNRC2 s and their transcripts are depicted. The black and gray boxes indicate an exon and UTRs, respectively, and the red box denotes the untranscribed UTR1 of the chimpanzee PNRC2 . Four different primers used to amplify PCR products are shown in red, blue, green, and black arrows, respectively. The target site duplications (TSDs) of the full-length chimpanzee-specific ERV are written in red. (C) The picture shows an agarose gel image of PCR products. Genomic DNAs from four different primates (human, chimpanzee, gorilla, and orangutan) were used as a DNA template for each PCR reaction.

    Journal: PLoS ONE

    Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome

    doi: 10.1371/journal.pone.0101195

    Figure Lengend Snippet: Alternative PNRC2 transcripts caused by a chimpanzee-specific ERV. (A) Using the UCSC Genome Browser, the PNRC2 gene is anatomized into intron, un-translated region (UTR), and coding exon. (B) The structures of chimpanzee and non-chimpanzee primate PNRC2 s and their transcripts are depicted. The black and gray boxes indicate an exon and UTRs, respectively, and the red box denotes the untranscribed UTR1 of the chimpanzee PNRC2 . Four different primers used to amplify PCR products are shown in red, blue, green, and black arrows, respectively. The target site duplications (TSDs) of the full-length chimpanzee-specific ERV are written in red. (C) The picture shows an agarose gel image of PCR products. Genomic DNAs from four different primates (human, chimpanzee, gorilla, and orangutan) were used as a DNA template for each PCR reaction.

    Article Snippet: We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of PCR master mix containing DNA polymerase (TaKaRa EmeraldAmp GT PCR Master Mix, TaKaRa, Japan).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis