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Promega polymerase chain reaction pcr master mix
Polymerase Chain Reaction Pcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr master mix/product/Promega
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr master mix - by Bioz Stars, 2020-08
90/100 stars

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Polymerase Chain Reaction:

Article Title: Low neutrophil alkaline phosphatase score is a new aspect of calreticulin-mutated myeloproliferative neoplasms
Article Snippet: .. DNA was amplified using polymerase chain reaction (PCR) Master Mix (Promega KK, Madison, WI, USA) as follows: 1 cycle at 95 °C for 2 min, 35 cycles of denaturation at 95 °C for 30 s, annealing at 52 °C (MPL , 60 °C) for 30 s, and extension at 72 °C for 30 s. PCR products were analyzed using 3 % agarose gel electrophoresis in the presence of ethidium bromide, and the amplicons were purified using Illustra ExoProStar (GE Healthcare Life Sciences, Buckinghamshire, UK). .. Direct sequencing was performed using the Big Dye Terminator ver3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and an ABI Prism 3130 Genetic Analyzer.

Article Title: Endogenous synthesis of prostacyclin was positively regulated during the maturation phase of cultured adipocytes
Article Snippet: .. M-MLV reverse transcriptase (RT) (Ribonuclease H minus, point mutant) and polymerase chain reaction (PCR) Master Mix were supplied by Promega (Madison, WI, USA). .. 3-Isobutyl-1-methylxanthine (IBMX), and Triglyceride E-Test Kit were purchased from Wako (Osaka, Japan).

Article Title: Karyotypic and mtDNA based characterization of Malaysian water buffalo
Article Snippet: .. The volume of reaction mixture used for optimization of DNA was 50 μl that consisted of 25 μl of polymerase chain reaction (PCR) Master Mix (Promega GoTaq Green Master Mix), 0.4 pmol of both forward and reverse primers and ~ 60 ng/ μl of extracted DNA. .. Amplification was done using Applied Bioscience Thermocycler following PCR profile; preliminary denaturation at 95 °C for 3 mins, followed by 35 cycles of 94 °C for 30 s, 58 °C for 1 min and 72 °C for 1 min.

Mutagenesis:

Article Title: Endogenous synthesis of prostacyclin was positively regulated during the maturation phase of cultured adipocytes
Article Snippet: .. M-MLV reverse transcriptase (RT) (Ribonuclease H minus, point mutant) and polymerase chain reaction (PCR) Master Mix were supplied by Promega (Madison, WI, USA). .. 3-Isobutyl-1-methylxanthine (IBMX), and Triglyceride E-Test Kit were purchased from Wako (Osaka, Japan).

Amplification:

Article Title: Low neutrophil alkaline phosphatase score is a new aspect of calreticulin-mutated myeloproliferative neoplasms
Article Snippet: .. DNA was amplified using polymerase chain reaction (PCR) Master Mix (Promega KK, Madison, WI, USA) as follows: 1 cycle at 95 °C for 2 min, 35 cycles of denaturation at 95 °C for 30 s, annealing at 52 °C (MPL , 60 °C) for 30 s, and extension at 72 °C for 30 s. PCR products were analyzed using 3 % agarose gel electrophoresis in the presence of ethidium bromide, and the amplicons were purified using Illustra ExoProStar (GE Healthcare Life Sciences, Buckinghamshire, UK). .. Direct sequencing was performed using the Big Dye Terminator ver3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and an ABI Prism 3130 Genetic Analyzer.

Agarose Gel Electrophoresis:

Article Title: Low neutrophil alkaline phosphatase score is a new aspect of calreticulin-mutated myeloproliferative neoplasms
Article Snippet: .. DNA was amplified using polymerase chain reaction (PCR) Master Mix (Promega KK, Madison, WI, USA) as follows: 1 cycle at 95 °C for 2 min, 35 cycles of denaturation at 95 °C for 30 s, annealing at 52 °C (MPL , 60 °C) for 30 s, and extension at 72 °C for 30 s. PCR products were analyzed using 3 % agarose gel electrophoresis in the presence of ethidium bromide, and the amplicons were purified using Illustra ExoProStar (GE Healthcare Life Sciences, Buckinghamshire, UK). .. Direct sequencing was performed using the Big Dye Terminator ver3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and an ABI Prism 3130 Genetic Analyzer.

Purification:

Article Title: Low neutrophil alkaline phosphatase score is a new aspect of calreticulin-mutated myeloproliferative neoplasms
Article Snippet: .. DNA was amplified using polymerase chain reaction (PCR) Master Mix (Promega KK, Madison, WI, USA) as follows: 1 cycle at 95 °C for 2 min, 35 cycles of denaturation at 95 °C for 30 s, annealing at 52 °C (MPL , 60 °C) for 30 s, and extension at 72 °C for 30 s. PCR products were analyzed using 3 % agarose gel electrophoresis in the presence of ethidium bromide, and the amplicons were purified using Illustra ExoProStar (GE Healthcare Life Sciences, Buckinghamshire, UK). .. Direct sequencing was performed using the Big Dye Terminator ver3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and an ABI Prism 3130 Genetic Analyzer.

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  • 94
    Promega pcr buffer
    Optimization of annealing temperature and Mg 2+ concentration for <t>PCR</t> with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target <t>DNA</t> for PCR was extracted from C. pneumoniae with the QIAmp DNA Mini Kit.
    Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 1066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Promega
    Average 94 stars, based on 1066 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    Promega pcr master mix
    Amplification pattern by <t>RT-PCR</t> with the site-specific primer pairs for intron-F and G . PCR products of from cDNA amplified with the primers <t>inF-F</t> and inF-R are eluted in lanes 2, 3, 15 and 16, and with primers inG-F and inG-R in lanes 4 and 5. PCR products from genomic DNA amplified with primer pair for intron-F are eluted in lanes 6, 7, 10, 13 and 14, and with primer pair for intron-G in lanes 8, 9 and 11. Lane 12 is the negative control.
    Pcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 980 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/Promega
    Average 92 stars, based on 980 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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    Optimization of annealing temperature and Mg 2+ concentration for PCR with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target DNA for PCR was extracted from C. pneumoniae with the QIAmp DNA Mini Kit.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Optimization of annealing temperature and Mg 2+ concentration for PCR with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target DNA for PCR was extracted from C. pneumoniae with the QIAmp DNA Mini Kit.

    Article Snippet: In brief, 2 μl of DNA extracts was processed in a 25-μl reaction volume containing PCR buffer (10 mM Tris [pH 9.0], 50 mM KCl, 0.01% gelatin), 200 μM deoxynucleoside triphosphates, 3.5 mM MgCl2 , 0.5 μM each primer, and 1 U of Taq polymerase (Promega, Madison, Wis.).

    Techniques: Concentration Assay, Polymerase Chain Reaction

    Detection sensitivity of PCR for omp1 versus the 16S rRNA gene. Two hundred microliters of the mock CSF containing a specific number of C. pneumoniae organisms (as indicated), was used for DNA extraction with the QIAmp DNA Mini Kit. A 2-μl portion of the 50-μl volume of DNA extracts was subjected to PCR. The optimized PCRs for omp1 and 16S rRNA gene were conducted (see Materials and Methods).

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Detection sensitivity of PCR for omp1 versus the 16S rRNA gene. Two hundred microliters of the mock CSF containing a specific number of C. pneumoniae organisms (as indicated), was used for DNA extraction with the QIAmp DNA Mini Kit. A 2-μl portion of the 50-μl volume of DNA extracts was subjected to PCR. The optimized PCRs for omp1 and 16S rRNA gene were conducted (see Materials and Methods).

    Article Snippet: In brief, 2 μl of DNA extracts was processed in a 25-μl reaction volume containing PCR buffer (10 mM Tris [pH 9.0], 50 mM KCl, 0.01% gelatin), 200 μM deoxynucleoside triphosphates, 3.5 mM MgCl2 , 0.5 μM each primer, and 1 U of Taq polymerase (Promega, Madison, Wis.).

    Techniques: Polymerase Chain Reaction, DNA Extraction

    Comparison of extraction efficacy of C. pneumoniae DNA from mock CSFs using two DNA extraction kits. The mock CSFs (200 μl) spiked with serially diluted bacteria were extracted either with the QIAmp DNA Mini Kit with a bacterial DNA extraction protocol or with the QIAmp DNA Blood Mini Kit. Two microliters of the extracted DNA (50 μl) was subjected to PCR with primers for omp1 . Results are representative of three experiments.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Comparison of extraction efficacy of C. pneumoniae DNA from mock CSFs using two DNA extraction kits. The mock CSFs (200 μl) spiked with serially diluted bacteria were extracted either with the QIAmp DNA Mini Kit with a bacterial DNA extraction protocol or with the QIAmp DNA Blood Mini Kit. Two microliters of the extracted DNA (50 μl) was subjected to PCR with primers for omp1 . Results are representative of three experiments.

    Article Snippet: In brief, 2 μl of DNA extracts was processed in a 25-μl reaction volume containing PCR buffer (10 mM Tris [pH 9.0], 50 mM KCl, 0.01% gelatin), 200 μM deoxynucleoside triphosphates, 3.5 mM MgCl2 , 0.5 μM each primer, and 1 U of Taq polymerase (Promega, Madison, Wis.).

    Techniques: DNA Extraction, Polymerase Chain Reaction

    Detection of C. pneumoniae DNA by PCR in CSFs obtained from patients with MS. Two hundred microliters of CSFs obtained from MS patients was processed for extraction of DNA utilizing the QIAmp DNA Mini Kit, and 2 μl of the resulting 50-μl DNA solution was subjected to PCR with primers for either omp1 or the 16S rRNA gene. Data are representative of three PCR experiments. Boxed numbers indicate CSFs obtained from the same patient at different time points. Each number is the sample number. M, molecular marker; PC, positive control for PCR; NC, negative control for PCR; IC, negative control for DNA extraction.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Detection of C. pneumoniae DNA by PCR in CSFs obtained from patients with MS. Two hundred microliters of CSFs obtained from MS patients was processed for extraction of DNA utilizing the QIAmp DNA Mini Kit, and 2 μl of the resulting 50-μl DNA solution was subjected to PCR with primers for either omp1 or the 16S rRNA gene. Data are representative of three PCR experiments. Boxed numbers indicate CSFs obtained from the same patient at different time points. Each number is the sample number. M, molecular marker; PC, positive control for PCR; NC, negative control for PCR; IC, negative control for DNA extraction.

    Article Snippet: In brief, 2 μl of DNA extracts was processed in a 25-μl reaction volume containing PCR buffer (10 mM Tris [pH 9.0], 50 mM KCl, 0.01% gelatin), 200 μM deoxynucleoside triphosphates, 3.5 mM MgCl2 , 0.5 μM each primer, and 1 U of Taq polymerase (Promega, Madison, Wis.).

    Techniques: Polymerase Chain Reaction, Mass Spectrometry, Marker, Positive Control, Negative Control, DNA Extraction

    Amplification pattern by RT-PCR with the site-specific primer pairs for intron-F and G . PCR products of from cDNA amplified with the primers inF-F and inF-R are eluted in lanes 2, 3, 15 and 16, and with primers inG-F and inG-R in lanes 4 and 5. PCR products from genomic DNA amplified with primer pair for intron-F are eluted in lanes 6, 7, 10, 13 and 14, and with primer pair for intron-G in lanes 8, 9 and 11. Lane 12 is the negative control.

    Journal: BMC Microbiology

    Article Title: Occurrence and characteristics of group 1 introns found at three different positions within the 28S ribosomal RNA gene of the dematiaceous Phialophora verrucosa: phylogenetic and secondary structural implications

    doi: 10.1186/1471-2180-11-94

    Figure Lengend Snippet: Amplification pattern by RT-PCR with the site-specific primer pairs for intron-F and G . PCR products of from cDNA amplified with the primers inF-F and inF-R are eluted in lanes 2, 3, 15 and 16, and with primers inG-F and inG-R in lanes 4 and 5. PCR products from genomic DNA amplified with primer pair for intron-F are eluted in lanes 6, 7, 10, 13 and 14, and with primer pair for intron-G in lanes 8, 9 and 11. Lane 12 is the negative control.

    Article Snippet: PCR was performed individually using PCR Master Mix and the primer pair inF-F and inF-R for intron-F and inG-F and inG-R for intron-G which we newly designed.

    Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Negative Control

    Schematic representation of the insertion deletion. (A) mms16 wild-type gene and different truncated fragments (I to III). Sizes are as indicated. (B) Molecular organization of the mms16 locus before and after insertion-duplication mutagenesis with a truncated fragment. Different fill patterns are used to mark the origins of different parts of the gene after a single crossover. Parts encoding the N and C termini of the corresponding gene products are indicated. (C) Characterization of the Da127 mutant by RT-PCR. The following primer combinations were applied in PCRs: mmpF1 plus mmpB1 (lanes 1 and 2); mmpF1 plus mmpB2 (lanes 3 and 4). A 271-bp truncated fragment was used for mutant construction. Positions of primers are indicated in panel A. cDNA obtained from the wild type (lanes 1 and 3) or the mutant strain Da127 (lanes 2 and 4) was used as a template. Identical reactions with reverse transcriptase omitted were used as negative controls (data not shown).

    Journal: Journal of Bacteriology

    Article Title: The Presumptive Magnetosome Protein Mms16 Is a Poly(3-Hydroxybutyrate) Granule-Bound Protein (Phasin) in Magnetospirillum gryphiswaldense

    doi: 10.1128/JB.187.7.2416-2425.2005

    Figure Lengend Snippet: Schematic representation of the insertion deletion. (A) mms16 wild-type gene and different truncated fragments (I to III). Sizes are as indicated. (B) Molecular organization of the mms16 locus before and after insertion-duplication mutagenesis with a truncated fragment. Different fill patterns are used to mark the origins of different parts of the gene after a single crossover. Parts encoding the N and C termini of the corresponding gene products are indicated. (C) Characterization of the Da127 mutant by RT-PCR. The following primer combinations were applied in PCRs: mmpF1 plus mmpB1 (lanes 1 and 2); mmpF1 plus mmpB2 (lanes 3 and 4). A 271-bp truncated fragment was used for mutant construction. Positions of primers are indicated in panel A. cDNA obtained from the wild type (lanes 1 and 3) or the mutant strain Da127 (lanes 2 and 4) was used as a template. Identical reactions with reverse transcriptase omitted were used as negative controls (data not shown).

    Article Snippet: The obtained cDNA was amplified by using PCR master mix (Promega) and primer pairs mmpF1 plus mmpB1 and mmpF2 plus mmpB2 , which amplify 309- and 367-bp fragments of the mms16 gene, respectively.

    Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction

    The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using Taq polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase PCR carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.

    Journal: PLoS ONE

    Article Title: Microarray Generation of Thousand-Member Oligonucleotide Libraries

    doi: 10.1371/journal.pone.0024906

    Figure Lengend Snippet: The generation of DNA templates from microarrays and parallel analysis. A ssDNA microarray was incubated with a primer (16 h) followed by elongation using Taq polymerase (16 h) producing as dsDNA microarray. The newly synthesized DNA strands were used as templates for solution phase PCR carried out over the microarray leading to amplification of the ssDNA displayed on the microarray. The dsDNA was amplified by PCR to produce fluorescently labeled ssDNA analogous to the ssDNA printed on the microarray. The fluorescently labeled ssDNA was hybridized to a complementary microarray or submitted to Solexa sequencing to allow decoding of the amplified ssDNA. FAM = 5(6)-carboxyfluorescein.

    Article Snippet: PCR in solution The purified products (250 ng) from each of the PCR “read-off” microarrays were used as templates in another round of PCR with primer-1 and 2 (1 µM) or Solexa-primer-1 and 2 (1 µM) in a 1× PCR Master Mix (200 µL; Promega, 25 U/mL Taq Polymerase, 200 µM dNTP, 1.5 mM MgCl2 ) in a Techne TC-312 PCR cycler with the same cycle as on the microarray.

    Techniques: Microarray, Incubation, Synthesized, Polymerase Chain Reaction, Amplification, Labeling, Sequencing