Structured Review

Arraystar polymerase chain reaction pcr master mix
Polymerase Chain Reaction Pcr Master Mix, supplied by Arraystar, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr master mix/product/Arraystar
Average 91 stars, based on 3 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr master mix - by Bioz Stars, 2020-08
91/100 stars

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Polymerase Chain Reaction:

Article Title: MiR-344b-1-3p targets TLR2 and negatively regulates TLR2 signaling pathway
Article Snippet: .. The 2× polymerase chain reaction (PCR) master mix was purchased from Arraystar (Rockville, MD, USA). ..

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. Reagents and instruments The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Isolation:

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. Reagents and instruments The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Injection:

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. Reagents and instruments The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Chromatin Immunoprecipitation:

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. Reagents and instruments The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Software:

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. Reagents and instruments The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

Article Title: Detection and Analysis of the Hedgehog Signaling Pathway-Related Long Non-Coding RNA (lncRNA) Expression Profiles in Keloid
Article Snippet: .. The LncPath™ Human Hedgehog (Hh) Pathway Array (ArrayStar, Rockville, MD, USA), RNA storage fluid (ComWin Biotech, Beijing, China), TRIzol, diethyl pyrocarbonate (DEPC) (Invitrogen, Carlsbad, CA, USA), gentamycin for injection (North China Pharmaceutical, Shijiazhuang, China), mRNA-ONLY™ mRNA isolation kit, sodium acetate, formaldehyde (Shanghai Chemical Reagent, Shanghai, China), ethylenediaminetetraacetic acid (EDTA), ethidium bromide (Cusabio Technology, Wuhan, China), agarose (Sangon Biotech, Shanghai, China), primers (ThermoFisher Scientific, Waltham, MA, USA), polymerase chain reaction (PCR) master mix, (ArrayStar, Rockville, MD, USA), Image-Pro Plus version 6.0 Image Analysis Software (Media Cybernetics, Rockville, MD, USA), chip scanner G2565CA, Feature Extraction v11.0.1.1, American GeneSpring (GX version 12) software (Agilent, Santa Clara CA, USA). ..

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    Arraystar pcr master mix
    <t>qRT-PCR-validated</t> ncRNA and mRNA expression changes. Microarray validation by qRT-PCR. Expression levels of four circRNAs, four miRNAs, and four mRNAs were determined by qRT-PCR. Each assay was performed at least three times. * p
    Pcr Master Mix, supplied by Arraystar, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/Arraystar
    Average 90 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

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    qRT-PCR-validated ncRNA and mRNA expression changes. Microarray validation by qRT-PCR. Expression levels of four circRNAs, four miRNAs, and four mRNAs were determined by qRT-PCR. Each assay was performed at least three times. * p

    Journal: Aging (Albany NY)

    Article Title: Identifying circRNA-associated-ceRNA networks in the hippocampus of Aβ1-42-induced Alzheimer's disease-like rats using microarray analysis

    doi: 10.18632/aging.101427

    Figure Lengend Snippet: qRT-PCR-validated ncRNA and mRNA expression changes. Microarray validation by qRT-PCR. Expression levels of four circRNAs, four miRNAs, and four mRNAs were determined by qRT-PCR. Each assay was performed at least three times. * p

    Article Snippet: An Applied Biosystems ViiA™7 Real-Time PCR System and 2× PCR Master Mix were used to perform qRT-PCR (Arraystar) in accordance with the manufacturer's instructions.

    Techniques: Quantitative RT-PCR, Expressing, Microarray

    qRT-PCR-validated ncRNA and mRNA expression changes. Microarray validation by qRT-PCR. Expression levels of four circRNAs, four miRNAs, and four mRNAs were determined by qRT-PCR. Each assay was performed at least three times. * p

    Journal: Aging (Albany NY)

    Article Title: Identifying circRNA-associated-ceRNA networks in the hippocampus of Aβ1-42-induced Alzheimer's disease-like rats using microarray analysis

    doi: 10.18632/aging.101427

    Figure Lengend Snippet: qRT-PCR-validated ncRNA and mRNA expression changes. Microarray validation by qRT-PCR. Expression levels of four circRNAs, four miRNAs, and four mRNAs were determined by qRT-PCR. Each assay was performed at least three times. * p

    Article Snippet: An Applied Biosystems ViiA™7 Real-Time PCR System and 2× PCR Master Mix were used to perform qRT-PCR (Arraystar) in accordance with the manufacturer's instructions.

    Techniques: Quantitative RT-PCR, Expressing, Microarray

    The differential expression of lncRNAs and mRNAs was validated by quantitative real-time PCR (qRT-PCR). The data showed that the expression levels of the lncRNAs BC092582 and MRAK050857 and the mRNA S100A8 were down-regulated and that the lncRNAs MRAK088596 and MRAK081790 and the mRNA MAPK10 were up-regulated in the hippocampal tissue samples from AD rats relative to the control rats. The heights of the columns in the chart represent FCs. The qRT-PCR results were consistent with the microarray data

    Journal: Molecular Neurobiology

    Article Title: Distinct Hippocampal Expression Profiles of Long Non-coding RNAs in an Alzheimer’s Disease Model

    doi: 10.1007/s12035-016-0038-5

    Figure Lengend Snippet: The differential expression of lncRNAs and mRNAs was validated by quantitative real-time PCR (qRT-PCR). The data showed that the expression levels of the lncRNAs BC092582 and MRAK050857 and the mRNA S100A8 were down-regulated and that the lncRNAs MRAK088596 and MRAK081790 and the mRNA MAPK10 were up-regulated in the hippocampal tissue samples from AD rats relative to the control rats. The heights of the columns in the chart represent FCs. The qRT-PCR results were consistent with the microarray data

    Article Snippet: An Applied Biosystems ViiA 7 Real-Time PCR System and 2 × PCR Master Mix were used to perform quantitative real-time (qRT)-PCR (Arraystar).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Microarray