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Bioneer Corporation polymerase chain reaction pcr kit
Polymerase Chain Reaction Pcr Kit, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr kit/product/Bioneer Corporation
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr kit - by Bioz Stars, 2020-04
86/100 stars

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Polymerase Chain Reaction:

Article Title: Nicotinic Acetylcholine Receptor ?7 and ?4 Subunits Contribute to Nicotine-Induced Apoptosis in Periodontal Ligament Stem Cells
Article Snippet: .. The reverse transcription (RT) products (50 ng) were then amplified using a polymerase chain reaction (PCR) kit (Bioneer) under the following conditions: denaturation at 94°C for 5 min followed by 30 cycles at 94°C for 45 s, 55°C for 30 s, and 72°C for 30 s followed by 5 min of extension at 72°C. ..

Isolation:

Article Title: Nicotinic Acetylcholine Receptor ?7 and ?4 Subunits Contribute to Nicotine-Induced Apoptosis in Periodontal Ligament Stem Cells
Article Snippet: Paragraph title: RNA isolation and reverse transcription-polymerase chain reaction ... The reverse transcription (RT) products (50 ng) were then amplified using a polymerase chain reaction (PCR) kit (Bioneer) under the following conditions: denaturation at 94°C for 5 min followed by 30 cycles at 94°C for 45 s, 55°C for 30 s, and 72°C for 30 s followed by 5 min of extension at 72°C.

Amplification:

Article Title: Nicotinic Acetylcholine Receptor ?7 and ?4 Subunits Contribute to Nicotine-Induced Apoptosis in Periodontal Ligament Stem Cells
Article Snippet: .. The reverse transcription (RT) products (50 ng) were then amplified using a polymerase chain reaction (PCR) kit (Bioneer) under the following conditions: denaturation at 94°C for 5 min followed by 30 cycles at 94°C for 45 s, 55°C for 30 s, and 72°C for 30 s followed by 5 min of extension at 72°C. ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: Nicotinic Acetylcholine Receptor ?7 and ?4 Subunits Contribute to Nicotine-Induced Apoptosis in Periodontal Ligament Stem Cells
Article Snippet: Paragraph title: RNA isolation and reverse transcription-polymerase chain reaction ... The reverse transcription (RT) products (50 ng) were then amplified using a polymerase chain reaction (PCR) kit (Bioneer) under the following conditions: denaturation at 94°C for 5 min followed by 30 cycles at 94°C for 45 s, 55°C for 30 s, and 72°C for 30 s followed by 5 min of extension at 72°C.

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  • 91
    Bioneer Corporation rna spin total rna extraction kit
    Inhibitory effect of WESS on RANKL-induced c-Fos and NFATc1 expression in BMMs. BMMs were pre-incubated with vehicle or WESS (25 μg/ml) for 3 h and further cultured in the presence of M-CSF (60 ng/ml) and RANKL (100 ng/ml) for 4 days. Total <t>RNA</t> and cell lysates were obtained at the indicated days. (A) c-Fos and NFATc1 mRNA levels were analyzed by <t>QPCR.</t> a P
    Rna Spin Total Rna Extraction Kit, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna spin total rna extraction kit/product/Bioneer Corporation
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    rna spin total rna extraction kit - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    95
    Bioneer Corporation dna extraction kit
    Detection of Leishmania spp. in the canine skin specimen by <t>PCR</t> with primer pairs specific to Leishmania spp. Lane M; 100 bp <t>DNA</t> molecular marker. Lane 1; negative control without template DNA used for PCR assay with primer pair specific to the partial SSU rRNA gene. Lane 2; the partial SSU rRNA gene. Lane 3; kinetoplast DNA minicircle of Leishmania infantum isolated from a dog from Korea in this study.
    Dna Extraction Kit, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna extraction kit/product/Bioneer Corporation
    Average 95 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    dna extraction kit - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    92
    Bioneer Corporation bioneer kits
    RT-qPCR detection of GRSPaV from total RNA isolated from grapevine leaves. Total RNA was isolated from V. vinifera var. Chardonnay using Spectrum™ Plant Total RNA kit (Sigma), Plant/fungi total RNA kit (Norgen) and AccuPrep viral RNA extraction kit <t>(Bioneer).</t> cDNA prepared with oligo d(T) on 2 μg of total RNA were subjected to SYBR Green quantitative real-time PCR with primers targeting GRSPaV capsid protein gene, grape actin1 and the gene encoding ubiquitin-60S ribosomal protein L40-2 (Additional file 1 : Table S1). Shown is the amplification plot ( a ) and melt curve ( b ) from cDNAs prepared from RNAs isolated with Sigma (blue), Norgen (purple) and Bioneer (green). The C q and melting temperature (T m ) of two technical replicates for all the samples and genes are given in the table below
    Bioneer Kits, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioneer kits/product/Bioneer Corporation
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bioneer kits - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Inhibitory effect of WESS on RANKL-induced c-Fos and NFATc1 expression in BMMs. BMMs were pre-incubated with vehicle or WESS (25 μg/ml) for 3 h and further cultured in the presence of M-CSF (60 ng/ml) and RANKL (100 ng/ml) for 4 days. Total RNA and cell lysates were obtained at the indicated days. (A) c-Fos and NFATc1 mRNA levels were analyzed by QPCR. a P

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Water extract of Spatholobus suberectus inhibits osteoclast differentiation and bone resorption

    doi: 10.1186/1472-6882-13-112

    Figure Lengend Snippet: Inhibitory effect of WESS on RANKL-induced c-Fos and NFATc1 expression in BMMs. BMMs were pre-incubated with vehicle or WESS (25 μg/ml) for 3 h and further cultured in the presence of M-CSF (60 ng/ml) and RANKL (100 ng/ml) for 4 days. Total RNA and cell lysates were obtained at the indicated days. (A) c-Fos and NFATc1 mRNA levels were analyzed by QPCR. a P

    Article Snippet: RNA-spin total RNA extraction kit, AccuPower RT-PreMix, and AccuPower GreenStar QPCR Master Mix were obtained from Bioneer (Daejeon, Korea).

    Techniques: Expressing, Incubation, Cell Culture, Real-time Polymerase Chain Reaction

    Detection of Leishmania spp. in the canine skin specimen by PCR with primer pairs specific to Leishmania spp. Lane M; 100 bp DNA molecular marker. Lane 1; negative control without template DNA used for PCR assay with primer pair specific to the partial SSU rRNA gene. Lane 2; the partial SSU rRNA gene. Lane 3; kinetoplast DNA minicircle of Leishmania infantum isolated from a dog from Korea in this study.

    Journal: The Korean Journal of Parasitology

    Article Title: An Autochthonous Case of Canine Visceral Leishmaniasis in Korea

    doi: 10.3347/kjp.2013.51.5.545

    Figure Lengend Snippet: Detection of Leishmania spp. in the canine skin specimen by PCR with primer pairs specific to Leishmania spp. Lane M; 100 bp DNA molecular marker. Lane 1; negative control without template DNA used for PCR assay with primer pair specific to the partial SSU rRNA gene. Lane 2; the partial SSU rRNA gene. Lane 3; kinetoplast DNA minicircle of Leishmania infantum isolated from a dog from Korea in this study.

    Article Snippet: For PCR, DNA was extracted from the specimen using a commercial DNA Extraction Kit (AccuPrep® DNA Extraction Kit, Bioneer, Daejeon, Korea) with minor modifications [ , ].

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Isolation

    RT-qPCR detection of GRSPaV from total RNA isolated from grapevine leaves. Total RNA was isolated from V. vinifera var. Chardonnay using Spectrum™ Plant Total RNA kit (Sigma), Plant/fungi total RNA kit (Norgen) and AccuPrep viral RNA extraction kit (Bioneer). cDNA prepared with oligo d(T) on 2 μg of total RNA were subjected to SYBR Green quantitative real-time PCR with primers targeting GRSPaV capsid protein gene, grape actin1 and the gene encoding ubiquitin-60S ribosomal protein L40-2 (Additional file 1 : Table S1). Shown is the amplification plot ( a ) and melt curve ( b ) from cDNAs prepared from RNAs isolated with Sigma (blue), Norgen (purple) and Bioneer (green). The C q and melting temperature (T m ) of two technical replicates for all the samples and genes are given in the table below

    Journal: Virology Journal

    Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

    doi: 10.1186/s12985-015-0376-3

    Figure Lengend Snippet: RT-qPCR detection of GRSPaV from total RNA isolated from grapevine leaves. Total RNA was isolated from V. vinifera var. Chardonnay using Spectrum™ Plant Total RNA kit (Sigma), Plant/fungi total RNA kit (Norgen) and AccuPrep viral RNA extraction kit (Bioneer). cDNA prepared with oligo d(T) on 2 μg of total RNA were subjected to SYBR Green quantitative real-time PCR with primers targeting GRSPaV capsid protein gene, grape actin1 and the gene encoding ubiquitin-60S ribosomal protein L40-2 (Additional file 1 : Table S1). Shown is the amplification plot ( a ) and melt curve ( b ) from cDNAs prepared from RNAs isolated with Sigma (blue), Norgen (purple) and Bioneer (green). The C q and melting temperature (T m ) of two technical replicates for all the samples and genes are given in the table below

    Article Snippet: The results of RT-qPCR with primers RSP8277F and RSP8436R targeting the capsid protein (CP) gene of GRSPaV (Additional file : Table S1) confirmed that the RNAs isolated with Sigma, Norgen and Bioneer kits are qualitatively and quantitatively satisfactory in the detection of GRSPaV in grapevine as indicated with low quantitation cycle (Cq) values and single, tight peaks on the melt curves (Fig. ).

    Techniques: Quantitative RT-PCR, Isolation, RNA Extraction, SYBR Green Assay, Real-time Polymerase Chain Reaction, Amplification

    PCR amplification of DNAs obtained from each protocol; A: Qiagen Kit; B: Bioneer Kit; C: proposed method; M: DNA Marker (50 bps).

    Journal: Journal of Family & Reproductive Health

    Article Title: Comparing the Efficiency of Three Protocols in Isolation of Cell Free Fetal DNA From Maternal Blood

    doi:

    Figure Lengend Snippet: PCR amplification of DNAs obtained from each protocol; A: Qiagen Kit; B: Bioneer Kit; C: proposed method; M: DNA Marker (50 bps).

    Article Snippet: The difference between Qiagen and Bioneer kits was meaningful for sequence 3 (p = 0.01) and in borderline significant difference for sequence 1 (p = 0.04).

    Techniques: Polymerase Chain Reaction, Amplification, Marker