polymerase chain reaction pcr genomic dna  (TaKaRa)

 
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    TaKaRa polymerase chain reaction pcr genomic dna
    Polymerase Chain Reaction Pcr Genomic Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr genomic dna/product/TaKaRa
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr genomic dna - by Bioz Stars, 2020-05
    88/100 stars

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    Polymerase Chain Reaction:

    Article Title: Seven novel mutations of ADAR in multi‐ethnic pedigrees with dyschromatosis symmetrica hereditaria in China. Seven novel mutations of ADAR in multi‐ethnic pedigrees with dyschromatosis symmetrica hereditaria in China
    Article Snippet: .. 2.2 Polymerase chain reaction (PCR) Genomic DNA samples were extracted from peripheral blood using Universal Genomic DNA Extraction kits (TaKaRa, Dalian, China). ..

    Article Title: New single nucleotide variation in the promoter region of androgen receptor (AR) gene in hypospadic patients
    Article Snippet: .. DNA extraction and polymerase chain reaction (PCR) Genomic DNA was extracted from peripheral blood of patients and controls using the whole blood extraction kit (Dr. Gen TLE, Takara, Japan). .. Nucleotides -756 to -366 of the AR 5’ promoter region (based on Ensemble.org genome browser) were amplified using the primer pair: 5’TCTCCAAA GCCACTAGGCAG3’ (sense) and 5’ACCGAA GAGGAAAGGGCAGCTC3’ (antisense).

    DNA Extraction:

    Article Title: Seven novel mutations of ADAR in multi‐ethnic pedigrees with dyschromatosis symmetrica hereditaria in China. Seven novel mutations of ADAR in multi‐ethnic pedigrees with dyschromatosis symmetrica hereditaria in China
    Article Snippet: .. 2.2 Polymerase chain reaction (PCR) Genomic DNA samples were extracted from peripheral blood using Universal Genomic DNA Extraction kits (TaKaRa, Dalian, China). ..

    Article Title: New single nucleotide variation in the promoter region of androgen receptor (AR) gene in hypospadic patients
    Article Snippet: .. DNA extraction and polymerase chain reaction (PCR) Genomic DNA was extracted from peripheral blood of patients and controls using the whole blood extraction kit (Dr. Gen TLE, Takara, Japan). .. Nucleotides -756 to -366 of the AR 5’ promoter region (based on Ensemble.org genome browser) were amplified using the primer pair: 5’TCTCCAAA GCCACTAGGCAG3’ (sense) and 5’ACCGAA GAGGAAAGGGCAGCTC3’ (antisense).

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    TaKaRa methylation specific pcr genomic dna extraction
    Methylation of the KLF4 promoter in cervical cancer cell lines. (A and B) KLF4 expression in the 4 cervical cancer cell-lines HeLa, CaSki, SiHa and C33A was detected by IHC (A) and by <t>PCR</t> and western blot (B). We applied the human embryonic stem cell line H7 as a positive control and the rabbit IgG polyclonal antibody as the isotype control in immunocytochemistry. (C) Bisulfite sequencing of the KLF4 promoter in cervical cancer cell-lines. (D) Statistical analysis of KLF4 promoter methylation in cervical cancer cell-lines. (E) MS-PCR for a region of the KLF4 promoter in the 4 cervical cancer cell lines. A methylated band was amplified in SiHa and C33A cells. Globally methylated <t>DNA</t> from normal fetal cord blood samples was included as a positive control for the methylated (M) and unmethylated (U) primers.
    Methylation Specific Pcr Genomic Dna Extraction, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methylation specific pcr genomic dna extraction/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    methylation specific pcr genomic dna extraction - by Bioz Stars, 2020-05
    86/100 stars
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    99
    TaKaRa genomic dna
    Real-time quantitative <t>PCR</t> validation of digital karyotyping data . Totally 52 normal individuals and 52 HCC samples were examined for genomic <t>DNA</t> level of (A) SGCE, (B) PEG10, (C) DYNC1I1 and (D) SLC25A13 respectively by real-time quantitative PCR. The purple horizontal line represented an average of genomic DNA level of each group. HCC samples which gained genomic amplification of examined genes were shown by red squares.
    Genomic Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna/product/TaKaRa
    Average 99 stars, based on 319 article reviews
    Price from $9.99 to $1999.99
    genomic dna - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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    Methylation of the KLF4 promoter in cervical cancer cell lines. (A and B) KLF4 expression in the 4 cervical cancer cell-lines HeLa, CaSki, SiHa and C33A was detected by IHC (A) and by PCR and western blot (B). We applied the human embryonic stem cell line H7 as a positive control and the rabbit IgG polyclonal antibody as the isotype control in immunocytochemistry. (C) Bisulfite sequencing of the KLF4 promoter in cervical cancer cell-lines. (D) Statistical analysis of KLF4 promoter methylation in cervical cancer cell-lines. (E) MS-PCR for a region of the KLF4 promoter in the 4 cervical cancer cell lines. A methylated band was amplified in SiHa and C33A cells. Globally methylated DNA from normal fetal cord blood samples was included as a positive control for the methylated (M) and unmethylated (U) primers.

    Journal: PLoS ONE

    Article Title: Promoter Hypermethylation of KLF4 Inactivates Its Tumor Suppressor Function in Cervical Carcinogenesis

    doi: 10.1371/journal.pone.0088827

    Figure Lengend Snippet: Methylation of the KLF4 promoter in cervical cancer cell lines. (A and B) KLF4 expression in the 4 cervical cancer cell-lines HeLa, CaSki, SiHa and C33A was detected by IHC (A) and by PCR and western blot (B). We applied the human embryonic stem cell line H7 as a positive control and the rabbit IgG polyclonal antibody as the isotype control in immunocytochemistry. (C) Bisulfite sequencing of the KLF4 promoter in cervical cancer cell-lines. (D) Statistical analysis of KLF4 promoter methylation in cervical cancer cell-lines. (E) MS-PCR for a region of the KLF4 promoter in the 4 cervical cancer cell lines. A methylated band was amplified in SiHa and C33A cells. Globally methylated DNA from normal fetal cord blood samples was included as a positive control for the methylated (M) and unmethylated (U) primers.

    Article Snippet: Bisulfite Sequencing and Methylation-Specific PCR Genomic DNA extraction was performed using the TaKaRa Genomic DNA Extraction Kit (TaKaRa Co., Dalian, China).

    Techniques: Methylation, Expressing, Immunohistochemistry, Polymerase Chain Reaction, Western Blot, Positive Control, Immunocytochemistry, Methylation Sequencing, Mass Spectrometry, Amplification

    Real-time quantitative PCR validation of digital karyotyping data . Totally 52 normal individuals and 52 HCC samples were examined for genomic DNA level of (A) SGCE, (B) PEG10, (C) DYNC1I1 and (D) SLC25A13 respectively by real-time quantitative PCR. The purple horizontal line represented an average of genomic DNA level of each group. HCC samples which gained genomic amplification of examined genes were shown by red squares.

    Journal: BMC Medical Genomics

    Article Title: Digital karyotyping reveals probable target genes at 7q21.3 locus in hepatocellular carcinoma

    doi: 10.1186/1755-8794-4-60

    Figure Lengend Snippet: Real-time quantitative PCR validation of digital karyotyping data . Totally 52 normal individuals and 52 HCC samples were examined for genomic DNA level of (A) SGCE, (B) PEG10, (C) DYNC1I1 and (D) SLC25A13 respectively by real-time quantitative PCR. The purple horizontal line represented an average of genomic DNA level of each group. HCC samples which gained genomic amplification of examined genes were shown by red squares.

    Article Snippet: PCR reaction mixture of 25 μl was composed of 50 ng genomic DNA, 12.5 μl SYBR Premix Ex Taq (TaKaRa), 250 nM of each primer and appropriate volume of ddH2 O.

    Techniques: Real-time Polymerase Chain Reaction, Amplification

    PCR amplicons of the Amelogenin and Sex determining region Y (SRY) gene from sperm genomic DNA. A. Schematic illustration of annealing of primers for specific amplification of X and Y chromosome in PCR reaction and B. A 167-bp and 107-bp fragment was amplified in PCR reaction from SRY and Amelogenin gene as indicators for sex determination. PCR; Polymerase chain reaction.

    Journal: International Journal of Fertility & Sterility

    Article Title: Zeta Sperm Selection Improves Pregnancy Rate and Alters Sex Ratio in Male Factor Infertility Patients: A Double-Blind, Randomized Clinical Trial

    doi:

    Figure Lengend Snippet: PCR amplicons of the Amelogenin and Sex determining region Y (SRY) gene from sperm genomic DNA. A. Schematic illustration of annealing of primers for specific amplification of X and Y chromosome in PCR reaction and B. A 167-bp and 107-bp fragment was amplified in PCR reaction from SRY and Amelogenin gene as indicators for sex determination. PCR; Polymerase chain reaction.

    Article Snippet: PCR was conducted by adding 1 µL genomic DNA to the 20 µL of PCR mixture that contained 1 ×SYBR® Premix Ex TaqTM (Takara Bio Inc., Otsu, Japan), 0.4 µM of each specific primer, and DNase-free water.

    Techniques: Polymerase Chain Reaction, Amplification

    The rs1550117 A > G variant affects the transcription factor binding affinity ( A ) Computational analysis predicted transcription factors for DNMT3A rs1550117 A > G variant. ( B ) ChIP assays using Hek293 and two NSCLC tissue samples. The presence of the SP1-binding DNMT3A promoter was verified by PCR. ( C ) SPR analysis comparing the binding affinity of Hek293 nuclear extracts or SP1 recombinant protein to DNA probes containing either the rs1550117 A or G alleles. ( D ) A luciferase construct containing either the A or G allele was co-transfected with pcDNA3.1-basic (control) or pcDNA3.1-SP1 expression plasmids in A549, PC14 and Hek293 cells.

    Journal: Oncotarget

    Article Title: The rs1550117 A > G variant in DNMT3A gene promoter significantly increases non-small cell lung cancer susceptibility in a Han Chinese population

    doi: 10.18632/oncotarget.15625

    Figure Lengend Snippet: The rs1550117 A > G variant affects the transcription factor binding affinity ( A ) Computational analysis predicted transcription factors for DNMT3A rs1550117 A > G variant. ( B ) ChIP assays using Hek293 and two NSCLC tissue samples. The presence of the SP1-binding DNMT3A promoter was verified by PCR. ( C ) SPR analysis comparing the binding affinity of Hek293 nuclear extracts or SP1 recombinant protein to DNA probes containing either the rs1550117 A or G alleles. ( D ) A luciferase construct containing either the A or G allele was co-transfected with pcDNA3.1-basic (control) or pcDNA3.1-SP1 expression plasmids in A549, PC14 and Hek293 cells.

    Article Snippet: The PCR reaction was performed in a total of 15μl containing 50ng genomic DNA, 1.5 μl 10× Taq Buffer (Mg2+ Plus), 0.2 μl 10 mM dNTP, 1 μl 1 mM Primer (forward: 5′-ACACACCGC CCTCACCCCTT-3′; reverse: 5′-TCCAGCAATCCC TGCCCACA-3′), and 0.5U Taq polymerase (Takara Biotechnology Co. Ltd, Dalian, China).

    Techniques: Variant Assay, Binding Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, SPR Assay, Recombinant, Luciferase, Construct, Transfection, Expressing