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Eurofins polymerase chain reaction pcr fragments
Polymerase Chain Reaction Pcr Fragments, supplied by Eurofins, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr fragments - by Bioz Stars, 2020-08
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Polymerase Chain Reaction:

Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
Article Snippet: .. Afterwards polymerase-chain reaction (PCR) fragments were produced applying universal primers ITS1 (5′-TCCGTAGGTGAACCTGCG-3′) (Eurofins MWG GmbH, Ebersberg, Germany) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) (Eurofins MWG GmbH, Ebersberg, Germany) (Fujita et al. [ ]). .. PCR amplification was performed in a total volume of 50 μl.

Produced:

Article Title: Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis
Article Snippet: .. Afterwards polymerase-chain reaction (PCR) fragments were produced applying universal primers ITS1 (5′-TCCGTAGGTGAACCTGCG-3′) (Eurofins MWG GmbH, Ebersberg, Germany) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) (Eurofins MWG GmbH, Ebersberg, Germany) (Fujita et al. [ ]). .. PCR amplification was performed in a total volume of 50 μl.

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  • 85
    Eurofins tβri promoter fragment
    Overexpression of <t>TβRI</t> mimics the forskolin effect on TGFβ-driven gene expression. MDA-MB-231 cells were either transfected with 1 µg of an expression plasmid encoding a constitutively active form of TβRI, TβRI(T204D), or mock-transfected or treated with forskolin. After o/n incubation in 2D cultures cells were analyzed for TβRI RNA levels by Q-RT-PCR (A) or for protein levels of TβRI, Cox-2, TIMP-1, PAI-1, Phospho-Smad3, ERK1/2 (loading control) by the Western blot technique (B). Each circle represents the mean value ± S.D. of three independent experiments. * p-value
    Tβri Promoter Fragment, supplied by Eurofins, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tβri promoter fragment/product/Eurofins
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tβri promoter fragment - by Bioz Stars, 2020-08
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    88
    Eurofins multiplex pcr restriction fragment length polymorphism rflp test
    Multiplex <t>PCR-RFLP.</t> a : Diagram demonstrating the introduction of MaeIII restriction sites by the mutation (11778A) and the combination of primer alterations and mutation (3460A, 14484C). Mutations are shown in red with the primer alterations in lower case. b : 2.5 % ethidium bromide stained agarose gel showing the results of the PCR-RFLP on patient DNA (black labels) and synthesised DNA controls (red labels). DNA was PCR amplified in a multiplex reaction using 3460 F/R, 11778 F/R and 14484 F/R and restricted using 1 unit of MaeIII as described. M: size marker; Uncut: non-restricted PCR products; All other lanes: patient (Black) or synthesised DNA controls (Red) containing the indicated mutation PCR amplified and restricted with MaeIII. The red arrow between the uncut and normal lanes demonstrates the internal control of restriction. Yellow arrows demonstrate mutation detection
    Multiplex Pcr Restriction Fragment Length Polymorphism Rflp Test, supplied by Eurofins, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex pcr restriction fragment length polymorphism rflp test/product/Eurofins
    Average 88 stars, based on 1 article reviews
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    multiplex pcr restriction fragment length polymorphism rflp test - by Bioz Stars, 2020-08
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    86
    Eurofins myd88 gene fragment
    Gel image showing polymerase chain reaction amplification of <t>Myd88</t> gene fragment. Lane 1, GeneRuler 100 bp DNA ladder; lane 2, 891 bp band indicative of Myd88 gene fragment.
    Myd88 Gene Fragment, supplied by Eurofins, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/myd88 gene fragment/product/Eurofins
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    92
    Eurofins pcr fragments
    Schematic representation of the construction of Δ lgt strains of E. coli carrying temperature-sensitive plasmid pMT-lgtVc(ts). <t>DNA</t> fragments carrying a deletion of lgt were generated by primerless <t>PCR</t> and cloned into suicide plasmid pMT-ssB. A kanamycin resistance gene flanked by loxP sites was then inserted in place of the deleted lgt gene. The resulting plasmids were used to create deletions in the chromosomes of E. coli by allelic exchange. The kanamycin resistance gene was then removed with Cre recombinase. A temperature-sensitive (ts) plasmid carrying a nonhomologous lgt gene complements the chromosomal deletion, allowing the survival of the strain at 30°C.
    Pcr Fragments, supplied by Eurofins, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 19 article reviews
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    pcr fragments - by Bioz Stars, 2020-08
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    Overexpression of TβRI mimics the forskolin effect on TGFβ-driven gene expression. MDA-MB-231 cells were either transfected with 1 µg of an expression plasmid encoding a constitutively active form of TβRI, TβRI(T204D), or mock-transfected or treated with forskolin. After o/n incubation in 2D cultures cells were analyzed for TβRI RNA levels by Q-RT-PCR (A) or for protein levels of TβRI, Cox-2, TIMP-1, PAI-1, Phospho-Smad3, ERK1/2 (loading control) by the Western blot technique (B). Each circle represents the mean value ± S.D. of three independent experiments. * p-value

    Journal: PLoS ONE

    Article Title: Cyclic AMP Enhances TGF? Responses of Breast Cancer Cells by Upregulating TGF? Receptor I Expression

    doi: 10.1371/journal.pone.0054261

    Figure Lengend Snippet: Overexpression of TβRI mimics the forskolin effect on TGFβ-driven gene expression. MDA-MB-231 cells were either transfected with 1 µg of an expression plasmid encoding a constitutively active form of TβRI, TβRI(T204D), or mock-transfected or treated with forskolin. After o/n incubation in 2D cultures cells were analyzed for TβRI RNA levels by Q-RT-PCR (A) or for protein levels of TβRI, Cox-2, TIMP-1, PAI-1, Phospho-Smad3, ERK1/2 (loading control) by the Western blot technique (B). Each circle represents the mean value ± S.D. of three independent experiments. * p-value

    Article Snippet: Correct sequence and positioning of the TβRI promoter fragment within the pGL4 vector were confirmed by DNA sequencing (MWG Eurofins).

    Techniques: Over Expression, Expressing, Multiple Displacement Amplification, Transfection, Plasmid Preparation, Incubation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    cAMP stimulates expression of TGFβ receptor I (TβRI). (A) cAMP increases the expression of TβRI, but not of TβRII. Cells were treated as indicated in 2D or 3D cultures for 24 h and analyzed for the expression of TβRI, and TβRII by Q-RT-PCR. When transfected with Flag-Smad3 (Smad) or mock-transfected (Ctrl) cells were incubated overnight before forskolin or vector (mock) was added. (B) TβRI levels are higher in 3D-cultured compared to 2D-cultured cells. Cells were grown in 2D or 3D cultures for 24 h and analyzed for TβRI-RNA and TβRII-RNA levels. Each bar represents the mean value ± S.D. of three independent experiments. (C) cAMP-dependent induction of TβRI expression is rapid. Cells were incubated with forskolin (FSK) or mock-treated in 2D cultures for 3 or 6 h before RNA expression of TβRI was analyzed by Q-RT-PCR. (D) cAMP stimulates TβRI expression also in BT-20 and MCF-7 breast cancer cells. Cells were incubated with forskolin or mock-treated for 24 h and analyzed for TβRI expression by Q-RT-PCR. Each bar represents the mean value ± S.D. of three independent experiments. * p-value

    Journal: PLoS ONE

    Article Title: Cyclic AMP Enhances TGF? Responses of Breast Cancer Cells by Upregulating TGF? Receptor I Expression

    doi: 10.1371/journal.pone.0054261

    Figure Lengend Snippet: cAMP stimulates expression of TGFβ receptor I (TβRI). (A) cAMP increases the expression of TβRI, but not of TβRII. Cells were treated as indicated in 2D or 3D cultures for 24 h and analyzed for the expression of TβRI, and TβRII by Q-RT-PCR. When transfected with Flag-Smad3 (Smad) or mock-transfected (Ctrl) cells were incubated overnight before forskolin or vector (mock) was added. (B) TβRI levels are higher in 3D-cultured compared to 2D-cultured cells. Cells were grown in 2D or 3D cultures for 24 h and analyzed for TβRI-RNA and TβRII-RNA levels. Each bar represents the mean value ± S.D. of three independent experiments. (C) cAMP-dependent induction of TβRI expression is rapid. Cells were incubated with forskolin (FSK) or mock-treated in 2D cultures for 3 or 6 h before RNA expression of TβRI was analyzed by Q-RT-PCR. (D) cAMP stimulates TβRI expression also in BT-20 and MCF-7 breast cancer cells. Cells were incubated with forskolin or mock-treated for 24 h and analyzed for TβRI expression by Q-RT-PCR. Each bar represents the mean value ± S.D. of three independent experiments. * p-value

    Article Snippet: Correct sequence and positioning of the TβRI promoter fragment within the pGL4 vector were confirmed by DNA sequencing (MWG Eurofins).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Transfection, Incubation, Plasmid Preparation, Cell Culture, RNA Expression

    The forskolin effect on TβRI requires transcription to be active, but is independent of CREB. (A) MDA-MB-231 cells were incubated with either actinomycin to block transcription or mock-treated for 24 h and analyzed for TβRI RNA levels by Q-RT-PCR. (B-D) Cells were transfected with either siCREB, siLuc or no siRNA, incubated for three days, treated for an additional 3 or 24 h with forskolin or mock (D) and analyzed for TβRI RNA (B, D) or protein levels (C) by Q-RT-PCR or by the Western blot technique (ns = non-specific band), respectively. (E) Cells were transfected with siSix1 (S) or siLuc (L), incubated for three days, treated with forskolin or mock o/n and analyzed for Six1 and TβRI RNA expression by Q-RT-PCR. (F) The TβRI promoter is responsive to cAMP. MDA-MB-231 cells were transfected with TβRI promoter/dual luciferase construct, incubated o/n and treated with forskolin (FSK) or mock for 6, 18 or 24 hours as indicated and analyzed for firefly and renilla (control) luciferase. (G) MDA-MB-231 cells were incubated with forskolin, HDACi III, forskolin plus HDACi III or mock-treated for 24 h and analyzed for TβRI expression by Q-RT-PCR. Each bar represents the mean value ± S.D. of 3 independent experiments. Relative promoter activity denotes the ratio of firefly to renilla luciferase activity. Each bar represents the mean value ± S.D. of 3–10 independent experiments. * p-value

    Journal: PLoS ONE

    Article Title: Cyclic AMP Enhances TGF? Responses of Breast Cancer Cells by Upregulating TGF? Receptor I Expression

    doi: 10.1371/journal.pone.0054261

    Figure Lengend Snippet: The forskolin effect on TβRI requires transcription to be active, but is independent of CREB. (A) MDA-MB-231 cells were incubated with either actinomycin to block transcription or mock-treated for 24 h and analyzed for TβRI RNA levels by Q-RT-PCR. (B-D) Cells were transfected with either siCREB, siLuc or no siRNA, incubated for three days, treated for an additional 3 or 24 h with forskolin or mock (D) and analyzed for TβRI RNA (B, D) or protein levels (C) by Q-RT-PCR or by the Western blot technique (ns = non-specific band), respectively. (E) Cells were transfected with siSix1 (S) or siLuc (L), incubated for three days, treated with forskolin or mock o/n and analyzed for Six1 and TβRI RNA expression by Q-RT-PCR. (F) The TβRI promoter is responsive to cAMP. MDA-MB-231 cells were transfected with TβRI promoter/dual luciferase construct, incubated o/n and treated with forskolin (FSK) or mock for 6, 18 or 24 hours as indicated and analyzed for firefly and renilla (control) luciferase. (G) MDA-MB-231 cells were incubated with forskolin, HDACi III, forskolin plus HDACi III or mock-treated for 24 h and analyzed for TβRI expression by Q-RT-PCR. Each bar represents the mean value ± S.D. of 3 independent experiments. Relative promoter activity denotes the ratio of firefly to renilla luciferase activity. Each bar represents the mean value ± S.D. of 3–10 independent experiments. * p-value

    Article Snippet: Correct sequence and positioning of the TβRI promoter fragment within the pGL4 vector were confirmed by DNA sequencing (MWG Eurofins).

    Techniques: Multiple Displacement Amplification, Incubation, Blocking Assay, Reverse Transcription Polymerase Chain Reaction, Transfection, Western Blot, RNA Expression, Luciferase, Construct, Expressing, Activity Assay

    Multiplex PCR-RFLP. a : Diagram demonstrating the introduction of MaeIII restriction sites by the mutation (11778A) and the combination of primer alterations and mutation (3460A, 14484C). Mutations are shown in red with the primer alterations in lower case. b : 2.5 % ethidium bromide stained agarose gel showing the results of the PCR-RFLP on patient DNA (black labels) and synthesised DNA controls (red labels). DNA was PCR amplified in a multiplex reaction using 3460 F/R, 11778 F/R and 14484 F/R and restricted using 1 unit of MaeIII as described. M: size marker; Uncut: non-restricted PCR products; All other lanes: patient (Black) or synthesised DNA controls (Red) containing the indicated mutation PCR amplified and restricted with MaeIII. The red arrow between the uncut and normal lanes demonstrates the internal control of restriction. Yellow arrows demonstrate mutation detection

    Journal: Eye and Vision

    Article Title: Development and validation of a novel PCR-RFLP based method for the detection of 3 primary mitochondrial mutations in Leber's hereditary optic neuropathy patients

    doi: 10.1186/s40662-015-0028-0

    Figure Lengend Snippet: Multiplex PCR-RFLP. a : Diagram demonstrating the introduction of MaeIII restriction sites by the mutation (11778A) and the combination of primer alterations and mutation (3460A, 14484C). Mutations are shown in red with the primer alterations in lower case. b : 2.5 % ethidium bromide stained agarose gel showing the results of the PCR-RFLP on patient DNA (black labels) and synthesised DNA controls (red labels). DNA was PCR amplified in a multiplex reaction using 3460 F/R, 11778 F/R and 14484 F/R and restricted using 1 unit of MaeIII as described. M: size marker; Uncut: non-restricted PCR products; All other lanes: patient (Black) or synthesised DNA controls (Red) containing the indicated mutation PCR amplified and restricted with MaeIII. The red arrow between the uncut and normal lanes demonstrates the internal control of restriction. Yellow arrows demonstrate mutation detection

    Article Snippet: Synthetic control DNA To provide an unlimited, reliable and patient-free resource for LHON testing across all current testing platforms as well as to allow for the development of the multiplex PCR-restriction fragment length polymorphism (RFLP) test described in this study, LHON control sequences were synthesised and cloned into standard plasmids by Eurofins Genomics (London, UK) or Life Technologies (Carlsbad, USA) based on the reference sequence NC_012920.1.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Mutagenesis, Staining, Agarose Gel Electrophoresis, Amplification, Marker

    Detection of heteroplasmy using multiplex PCR-RFLP. 2.5 % ethidium bromide stained agarose gel showing the results of the multiplex PCR-RFLP on patient DNA heteroplasmic for G11778A mutation. M: size marker; 11778A-1: patient sample approximately 90 % 11778A; 11778A-2: patient sample approximately 10 % 11778A

    Journal: Eye and Vision

    Article Title: Development and validation of a novel PCR-RFLP based method for the detection of 3 primary mitochondrial mutations in Leber's hereditary optic neuropathy patients

    doi: 10.1186/s40662-015-0028-0

    Figure Lengend Snippet: Detection of heteroplasmy using multiplex PCR-RFLP. 2.5 % ethidium bromide stained agarose gel showing the results of the multiplex PCR-RFLP on patient DNA heteroplasmic for G11778A mutation. M: size marker; 11778A-1: patient sample approximately 90 % 11778A; 11778A-2: patient sample approximately 10 % 11778A

    Article Snippet: Synthetic control DNA To provide an unlimited, reliable and patient-free resource for LHON testing across all current testing platforms as well as to allow for the development of the multiplex PCR-restriction fragment length polymorphism (RFLP) test described in this study, LHON control sequences were synthesised and cloned into standard plasmids by Eurofins Genomics (London, UK) or Life Technologies (Carlsbad, USA) based on the reference sequence NC_012920.1.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Mutagenesis, Marker

    Workflow and expected results for the Multiplex PCR-RFLP. a : Sizes of PCR products generated in the multiplex PCR with products of 333 bp, 164 bp, and 236 bp. b : Size of restriction products generated by MaeIII restriction of the multiplex PCR products. In non-mutated samples, only the internal control of restriction in the 3460 product is restricted resulting in the removal of 26 bp from the 333 bp PCR product. c : Schematic representation of the band pattern expected from the diagnostic test. DNA samples with the 3460A, 11778A and 14484C mutations restricted with MaeIII yield the restriction products indicated. The red arrow indicates the control of restriction and the yellow arrows indicate mutation detection

    Journal: Eye and Vision

    Article Title: Development and validation of a novel PCR-RFLP based method for the detection of 3 primary mitochondrial mutations in Leber's hereditary optic neuropathy patients

    doi: 10.1186/s40662-015-0028-0

    Figure Lengend Snippet: Workflow and expected results for the Multiplex PCR-RFLP. a : Sizes of PCR products generated in the multiplex PCR with products of 333 bp, 164 bp, and 236 bp. b : Size of restriction products generated by MaeIII restriction of the multiplex PCR products. In non-mutated samples, only the internal control of restriction in the 3460 product is restricted resulting in the removal of 26 bp from the 333 bp PCR product. c : Schematic representation of the band pattern expected from the diagnostic test. DNA samples with the 3460A, 11778A and 14484C mutations restricted with MaeIII yield the restriction products indicated. The red arrow indicates the control of restriction and the yellow arrows indicate mutation detection

    Article Snippet: Synthetic control DNA To provide an unlimited, reliable and patient-free resource for LHON testing across all current testing platforms as well as to allow for the development of the multiplex PCR-restriction fragment length polymorphism (RFLP) test described in this study, LHON control sequences were synthesised and cloned into standard plasmids by Eurofins Genomics (London, UK) or Life Technologies (Carlsbad, USA) based on the reference sequence NC_012920.1.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Generated, Diagnostic Assay, Mutagenesis

    Gel image showing polymerase chain reaction amplification of Myd88 gene fragment. Lane 1, GeneRuler 100 bp DNA ladder; lane 2, 891 bp band indicative of Myd88 gene fragment.

    Journal: Clinical and Experimental Vaccine Research

    Article Title: Immunogenicity and efficacy of a plasmid DNA rabies vaccine incorporating Myd88 as a genetic adjuvant

    doi: 10.7774/cevr.2014.3.2.202

    Figure Lengend Snippet: Gel image showing polymerase chain reaction amplification of Myd88 gene fragment. Lane 1, GeneRuler 100 bp DNA ladder; lane 2, 891 bp band indicative of Myd88 gene fragment.

    Article Snippet: Primers to amplify glycoprotein and Myd88 gene fragment were designed from sequences available at NCBI and the plasmid sequence, respectively, using PrimeGen Software (V2 version), and were synthesized at Eurofins Genomics India Pvt.

    Techniques: Polymerase Chain Reaction, Amplification

    Images of BHK-21 cells fluorescently stained for expression of G and MyD88. Cells were grown in 24-well plates and mock-transfected (A, B) or transfected with a liposomal complex of pIRES-Rgp-Myd (C, D). Forty-eight hours later, the cells were stained sequentially with a murine anti-G antibody and a rabbit polyclonal anti-Myd88 antibody, followed by species-specific secondary IgG conjugated with FITC or TRITC, respectively. Note the lack of specific fluorescence in mock-transfected cells, and the apple-green fluorescence (indicative of expression of G) and orange-red fluorescence (indicative of MyD88 expression) in pIRES-Rgp-Myd transfected cells, following staining (images at a total magnification of ×400).

    Journal: Clinical and Experimental Vaccine Research

    Article Title: Immunogenicity and efficacy of a plasmid DNA rabies vaccine incorporating Myd88 as a genetic adjuvant

    doi: 10.7774/cevr.2014.3.2.202

    Figure Lengend Snippet: Images of BHK-21 cells fluorescently stained for expression of G and MyD88. Cells were grown in 24-well plates and mock-transfected (A, B) or transfected with a liposomal complex of pIRES-Rgp-Myd (C, D). Forty-eight hours later, the cells were stained sequentially with a murine anti-G antibody and a rabbit polyclonal anti-Myd88 antibody, followed by species-specific secondary IgG conjugated with FITC or TRITC, respectively. Note the lack of specific fluorescence in mock-transfected cells, and the apple-green fluorescence (indicative of expression of G) and orange-red fluorescence (indicative of MyD88 expression) in pIRES-Rgp-Myd transfected cells, following staining (images at a total magnification of ×400).

    Article Snippet: Primers to amplify glycoprotein and Myd88 gene fragment were designed from sequences available at NCBI and the plasmid sequence, respectively, using PrimeGen Software (V2 version), and were synthesized at Eurofins Genomics India Pvt.

    Techniques: Staining, Expressing, Transfection, Fluorescence

    Schematic representation of the construction of Δ lgt strains of E. coli carrying temperature-sensitive plasmid pMT-lgtVc(ts). DNA fragments carrying a deletion of lgt were generated by primerless PCR and cloned into suicide plasmid pMT-ssB. A kanamycin resistance gene flanked by loxP sites was then inserted in place of the deleted lgt gene. The resulting plasmids were used to create deletions in the chromosomes of E. coli by allelic exchange. The kanamycin resistance gene was then removed with Cre recombinase. A temperature-sensitive (ts) plasmid carrying a nonhomologous lgt gene complements the chromosomal deletion, allowing the survival of the strain at 30°C.

    Journal: Applied and Environmental Microbiology

    Article Title: A Novel Nonantibiotic, lgt-Based Selection System for Stable Maintenance of Expression Vectors in Escherichia coli and Vibrio cholerae

    doi: 10.1128/AEM.02143-17

    Figure Lengend Snippet: Schematic representation of the construction of Δ lgt strains of E. coli carrying temperature-sensitive plasmid pMT-lgtVc(ts). DNA fragments carrying a deletion of lgt were generated by primerless PCR and cloned into suicide plasmid pMT-ssB. A kanamycin resistance gene flanked by loxP sites was then inserted in place of the deleted lgt gene. The resulting plasmids were used to create deletions in the chromosomes of E. coli by allelic exchange. The kanamycin resistance gene was then removed with Cre recombinase. A temperature-sensitive (ts) plasmid carrying a nonhomologous lgt gene complements the chromosomal deletion, allowing the survival of the strain at 30°C.

    Article Snippet: All DNA sequencing of PCR fragments and plasmids was performed by Eurofins Genomics (Ebersberg, Germany).

    Techniques: Plasmid Preparation, Generated, Polymerase Chain Reaction, Clone Assay