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Bio-Rad polymerase chain reaction pcr fragment
Real-time <t>RT-PCR</t> analyses of <t>tHsc70</t> expression in the liver (a), skeletal muscle (b), lung (c), and heart (d) of P . sinensis after heat stress for 1, 2, and 4 h with 1-h adaptive recovery at 25 °C (Groups V, VI, and VII) or without adaptation
Polymerase Chain Reaction Pcr Fragment, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress *"

Article Title: Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress *

Journal: Journal of Zhejiang University. Science. B

doi: 10.1631/jzus.B1100309

Real-time RT-PCR analyses of tHsc70 expression in the liver (a), skeletal muscle (b), lung (c), and heart (d) of P . sinensis after heat stress for 1, 2, and 4 h with 1-h adaptive recovery at 25 °C (Groups V, VI, and VII) or without adaptation
Figure Legend Snippet: Real-time RT-PCR analyses of tHsc70 expression in the liver (a), skeletal muscle (b), lung (c), and heart (d) of P . sinensis after heat stress for 1, 2, and 4 h with 1-h adaptive recovery at 25 °C (Groups V, VI, and VII) or without adaptation

Techniques Used: Quantitative RT-PCR, Expressing

Semi-quantitative RT-PCR analysis of tHsc70 expression in the liver, skeletal muscle, lung, and heart of P . sinensis
Figure Legend Snippet: Semi-quantitative RT-PCR analysis of tHsc70 expression in the liver, skeletal muscle, lung, and heart of P . sinensis

Techniques Used: Quantitative RT-PCR, Expressing

Related Articles

Polymerase Chain Reaction:

Article Title: Penicillin-Binding Protein 2x of Streptococcus pneumoniae: The Mutation Ala707Asp Within the C-terminal PASTA2 Domain Leads to Destabilization
Article Snippet: .. To construct the plasmid pFP098, a polymerase chain reaction (PCR) fragment was amplified from strain DKL04 with the primers pbp2x_gfp_f/pbp2x_gfp_r using iProof high-fidelity DNA polymerase (Bio-Rad Laboratories). .. The amplified pbp2x fragment was digested with Not I and Spe I and inserted into the same sites in pJWV25, generating the plasmid pFP098.

Article Title: Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress *
Article Snippet: .. The polymerase chain reaction (PCR) fragment of tHsc70 was amplified using the primer pair Hsc70-dF and Hsc70-dR (Table ) on a thermal cycler S1000™ (Bio-Rad, Hercules, CA, USA). .. PCR was carried out using 2 μl of cDNA as a template in a 30-μl reaction mixture with a denaturation step of 95 °C for 3 min, followed by 35 cycles of 94 °C for 30 s, 50 °C for 50 s and 72 °C for 30 s, and completed with a 10-min extension at 72 °C.

Amplification:

Article Title: Penicillin-Binding Protein 2x of Streptococcus pneumoniae: The Mutation Ala707Asp Within the C-terminal PASTA2 Domain Leads to Destabilization
Article Snippet: .. To construct the plasmid pFP098, a polymerase chain reaction (PCR) fragment was amplified from strain DKL04 with the primers pbp2x_gfp_f/pbp2x_gfp_r using iProof high-fidelity DNA polymerase (Bio-Rad Laboratories). .. The amplified pbp2x fragment was digested with Not I and Spe I and inserted into the same sites in pJWV25, generating the plasmid pFP098.

Article Title: Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress *
Article Snippet: .. The polymerase chain reaction (PCR) fragment of tHsc70 was amplified using the primer pair Hsc70-dF and Hsc70-dR (Table ) on a thermal cycler S1000™ (Bio-Rad, Hercules, CA, USA). .. PCR was carried out using 2 μl of cDNA as a template in a 30-μl reaction mixture with a denaturation step of 95 °C for 3 min, followed by 35 cycles of 94 °C for 30 s, 50 °C for 50 s and 72 °C for 30 s, and completed with a 10-min extension at 72 °C.

Construct:

Article Title: Penicillin-Binding Protein 2x of Streptococcus pneumoniae: The Mutation Ala707Asp Within the C-terminal PASTA2 Domain Leads to Destabilization
Article Snippet: .. To construct the plasmid pFP098, a polymerase chain reaction (PCR) fragment was amplified from strain DKL04 with the primers pbp2x_gfp_f/pbp2x_gfp_r using iProof high-fidelity DNA polymerase (Bio-Rad Laboratories). .. The amplified pbp2x fragment was digested with Not I and Spe I and inserted into the same sites in pJWV25, generating the plasmid pFP098.

Plasmid Preparation:

Article Title: Penicillin-Binding Protein 2x of Streptococcus pneumoniae: The Mutation Ala707Asp Within the C-terminal PASTA2 Domain Leads to Destabilization
Article Snippet: .. To construct the plasmid pFP098, a polymerase chain reaction (PCR) fragment was amplified from strain DKL04 with the primers pbp2x_gfp_f/pbp2x_gfp_r using iProof high-fidelity DNA polymerase (Bio-Rad Laboratories). .. The amplified pbp2x fragment was digested with Not I and Spe I and inserted into the same sites in pJWV25, generating the plasmid pFP098.

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    Bio-Rad cdna fragment levels
    Comparison different PCR methods and impact on quantification. Graphic representation of the quantification of dystrophin <t>cDNA</t> construct templates lacking or containing exon 51 mixed in different ratios ranging from 0% to 100% using nested PCR (40, 50 and 60 cycles), <t>qPCR</t> (LinRegPCR and Pfaffl methods) and ddPCR. The dashed red line indicates the theoretical template ratios. The solid black ddPCR line is almost indistinguishable from the theoretical line and both lines are superimposed. Data is based on one experiment using 2 replicates for ddPCR and 3 replicates for qPCR.
    Cdna Fragment Levels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad pcr fragments
    (A) <t>PCR-DGGE</t> profiles representing the bacterial diversity in baby L generated from samples taken from birth to 372 days. Both the day and the approximate time of the month of sampling are indicated. Changes in the diet, such as addition of formula milk, are indicated by arrows. The bands identified from the 16S rDNA clone libraries are numbered and are indicated by arrowheads; these bands are described in the table in panel B. (B) Closest relative as determined by comparative sequence analysis, level of identity with this relative, clone designation, and accession number for each band identified in panel A.
    Pcr Fragments, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad p1ap p1 bp pcr fragment
    A. <t>PCR</t> screening, using primers P1D and P1test, of plaques obtained upon phage recombineering. Lanes 4 and 11 contain PCR products that indicate the presence of P1virΔIS within the plaques. B. PCR verification of the shortening of the IS‐deleted region in a pure P1virΔIS lysate using primers P1D and P1 BP. M: 1 kb DNA ladder (Fermentas).
    P1ap P1 Bp Pcr Fragment, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad dna fragments
    NEK2 regulates heparanase expression via NF-κB. ( A ) Total RNA from EV or NEK2-OE or NEK2-shRNA ARP1 cells were harvested for gene expression profiling; NEK2 and HPSE mRNA levels are presented as bar graphs of duplicate samples. ( B ) The map of p65 binding to the HPSE promoter was obtained from the UCSC genome browser ChIP-seq data (track name: GM12878+TNFa RELA). HSPE contains 2 RELA (p65) binding sites across its sequence. H3K4me3 and H3K27Ac, typical for promoter and activator binding elements, respectively, show strong peaks at the p65 binding site of the HPSE sequence (red box). ( C ) <t>ChIP-qPCR</t> was performed in control cell (EV) and NEK2-OE ARP1 myeloma cells using p65 antibodies. The <t>DNA</t> occupancy of the HPSE promoter was analyzed by qPCR and a Student’s t test was performed to assess the significance. * P
    Dna Fragments, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Comparison different PCR methods and impact on quantification. Graphic representation of the quantification of dystrophin cDNA construct templates lacking or containing exon 51 mixed in different ratios ranging from 0% to 100% using nested PCR (40, 50 and 60 cycles), qPCR (LinRegPCR and Pfaffl methods) and ddPCR. The dashed red line indicates the theoretical template ratios. The solid black ddPCR line is almost indistinguishable from the theoretical line and both lines are superimposed. Data is based on one experiment using 2 replicates for ddPCR and 3 replicates for qPCR.

    Journal: PLoS ONE

    Article Title: Digital Droplet PCR for the Absolute Quantification of Exon Skipping Induced by Antisense Oligonucleotides in (Pre-)Clinical Development for Duchenne Muscular Dystrophy

    doi: 10.1371/journal.pone.0162467

    Figure Lengend Snippet: Comparison different PCR methods and impact on quantification. Graphic representation of the quantification of dystrophin cDNA construct templates lacking or containing exon 51 mixed in different ratios ranging from 0% to 100% using nested PCR (40, 50 and 60 cycles), qPCR (LinRegPCR and Pfaffl methods) and ddPCR. The dashed red line indicates the theoretical template ratios. The solid black ddPCR line is almost indistinguishable from the theoretical line and both lines are superimposed. Data is based on one experiment using 2 replicates for ddPCR and 3 replicates for qPCR.

    Article Snippet: In each qPCR run, a no template control (NTC) was included as negative control. cDNA fragment levels were determined on a CFX96 real-time thermal cycler (BioRad) using the following protocol: 95°C for 10 min, then 40 cycles of 95°C for 15 sec and 60°C for 1 min. qPCR data was analyzed using both the Pfaffl method [ ] and the LinRegPCR method [ ].

    Techniques: Polymerase Chain Reaction, Construct, Nested PCR, Real-time Polymerase Chain Reaction

    (A) PCR-DGGE profiles representing the bacterial diversity in baby L generated from samples taken from birth to 372 days. Both the day and the approximate time of the month of sampling are indicated. Changes in the diet, such as addition of formula milk, are indicated by arrows. The bands identified from the 16S rDNA clone libraries are numbered and are indicated by arrowheads; these bands are described in the table in panel B. (B) Closest relative as determined by comparative sequence analysis, level of identity with this relative, clone designation, and accession number for each band identified in panel A.

    Journal: Applied and Environmental Microbiology

    Article Title: Molecular Monitoring of Succession of Bacterial Communities in Human Neonates

    doi: 10.1128/AEM.68.1.219-226.2002

    Figure Lengend Snippet: (A) PCR-DGGE profiles representing the bacterial diversity in baby L generated from samples taken from birth to 372 days. Both the day and the approximate time of the month of sampling are indicated. Changes in the diet, such as addition of formula milk, are indicated by arrows. The bands identified from the 16S rDNA clone libraries are numbered and are indicated by arrowheads; these bands are described in the table in panel B. (B) Closest relative as determined by comparative sequence analysis, level of identity with this relative, clone designation, and accession number for each band identified in panel A.

    Article Snippet: PCR fragments, also called amplicons, were separated by DGGE by using the specifications of Muyzer et al. ( ) and the Decode system (Bio-Rad Laboratories, Hercules, Calif.), with the following modifications.

    Techniques: Polymerase Chain Reaction, Denaturing Gradient Gel Electrophoresis, Generated, Sampling, Sequencing

    (A) PCR-DGGE profiles representing the bacterial diversity in baby D generated from samples taken from birth to 323 days. Both the day and the approximate time of the month of sampling are indicated. Changes in the diet are indicated by arrows. The bands identified from the 16S rDNA clone libraries are numbered and are indicated by arrowheads; these bands are described in the table in panel B. (B) Closest relative as determined by comparative sequence analysis, level of identity with this relative, clone designation, and accession number for each band identified in panel A.

    Journal: Applied and Environmental Microbiology

    Article Title: Molecular Monitoring of Succession of Bacterial Communities in Human Neonates

    doi: 10.1128/AEM.68.1.219-226.2002

    Figure Lengend Snippet: (A) PCR-DGGE profiles representing the bacterial diversity in baby D generated from samples taken from birth to 323 days. Both the day and the approximate time of the month of sampling are indicated. Changes in the diet are indicated by arrows. The bands identified from the 16S rDNA clone libraries are numbered and are indicated by arrowheads; these bands are described in the table in panel B. (B) Closest relative as determined by comparative sequence analysis, level of identity with this relative, clone designation, and accession number for each band identified in panel A.

    Article Snippet: PCR fragments, also called amplicons, were separated by DGGE by using the specifications of Muyzer et al. ( ) and the Decode system (Bio-Rad Laboratories, Hercules, Calif.), with the following modifications.

    Techniques: Polymerase Chain Reaction, Denaturing Gradient Gel Electrophoresis, Generated, Sampling, Sequencing

    A. PCR screening, using primers P1D and P1test, of plaques obtained upon phage recombineering. Lanes 4 and 11 contain PCR products that indicate the presence of P1virΔIS within the plaques. B. PCR verification of the shortening of the IS‐deleted region in a pure P1virΔIS lysate using primers P1D and P1 BP. M: 1 kb DNA ladder (Fermentas).

    Journal: Microbial biotechnology

    Article Title: Bacteriophage recombineering in the lytic state using the lambda red recombinases

    doi: 10.1111/j.1751-7915.2011.00292.x

    Figure Lengend Snippet: A. PCR screening, using primers P1D and P1test, of plaques obtained upon phage recombineering. Lanes 4 and 11 contain PCR products that indicate the presence of P1virΔIS within the plaques. B. PCR verification of the shortening of the IS‐deleted region in a pure P1virΔIS lysate using primers P1D and P1 BP. M: 1 kb DNA ladder (Fermentas).

    Article Snippet: A mixture containing 100 ng of phage DNA and 300 ng of the P1AP–P1 BP PCR fragment was electroporated into an aliquot of MG1655/pKD46 cells at a voltage of 1800 V in a 0.1 cm electroporation cuvette using a Bio‐Rad MicroPulser Electroporator.

    Techniques: Polymerase Chain Reaction

    NEK2 regulates heparanase expression via NF-κB. ( A ) Total RNA from EV or NEK2-OE or NEK2-shRNA ARP1 cells were harvested for gene expression profiling; NEK2 and HPSE mRNA levels are presented as bar graphs of duplicate samples. ( B ) The map of p65 binding to the HPSE promoter was obtained from the UCSC genome browser ChIP-seq data (track name: GM12878+TNFa RELA). HSPE contains 2 RELA (p65) binding sites across its sequence. H3K4me3 and H3K27Ac, typical for promoter and activator binding elements, respectively, show strong peaks at the p65 binding site of the HPSE sequence (red box). ( C ) ChIP-qPCR was performed in control cell (EV) and NEK2-OE ARP1 myeloma cells using p65 antibodies. The DNA occupancy of the HPSE promoter was analyzed by qPCR and a Student’s t test was performed to assess the significance. * P

    Journal: The Journal of Clinical Investigation

    Article Title: Destabilizing NEK2 overcomes resistance to proteasome inhibition in multiple myeloma

    doi: 10.1172/JCI98765

    Figure Lengend Snippet: NEK2 regulates heparanase expression via NF-κB. ( A ) Total RNA from EV or NEK2-OE or NEK2-shRNA ARP1 cells were harvested for gene expression profiling; NEK2 and HPSE mRNA levels are presented as bar graphs of duplicate samples. ( B ) The map of p65 binding to the HPSE promoter was obtained from the UCSC genome browser ChIP-seq data (track name: GM12878+TNFa RELA). HSPE contains 2 RELA (p65) binding sites across its sequence. H3K4me3 and H3K27Ac, typical for promoter and activator binding elements, respectively, show strong peaks at the p65 binding site of the HPSE sequence (red box). ( C ) ChIP-qPCR was performed in control cell (EV) and NEK2-OE ARP1 myeloma cells using p65 antibodies. The DNA occupancy of the HPSE promoter was analyzed by qPCR and a Student’s t test was performed to assess the significance. * P

    Article Snippet: The ChIPed DNA fragments were quantified by Qubit DNA assay kit (Life Technologies), and the enrichment of DNA fragments that contain putative NF-κB binding sites in the gene promoter was quantified by qPCR containing DNA fragments using a Two-Color Real-Time PCR Detection System (Bio-Rad).

    Techniques: Expressing, shRNA, Binding Assay, Chromatin Immunoprecipitation, Sequencing, Real-time Polymerase Chain Reaction