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MACHEREY NAGEL polymerase chain reaction pcr clean up kit
Polymerase Chain Reaction Pcr Clean Up Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr clean up kit/product/MACHEREY NAGEL
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr clean up kit - by Bioz Stars, 2020-09
92/100 stars

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Polymerase Chain Reaction:

Article Title: Identification of genes and pathways associated with MDR in MCF-7/MDR breast cancer cells by RNA-seq analysis
Article Snippet: .. First-strand reverse transcription (RT) primer: biotin, 5′-CAAGCAGAAGACGGCATACGAGTVN-3′ was conducted using a SuperScript III First-Strand Synthesis system (Thermo Fisher Scientific, Inc.) ( ) was used to synthesize the first-strand cDNA according to the manufacturer's protocols, followed by purification of cDNA with a NucleoSpin gel and polymerase chain reaction (PCR) Clean-up kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) to remove free RT primer. .. Dynabeads® MyOne™ Streptavidin C1 (cat. no. 650.01; Thermo Fisher Scientific, Inc.) were used for further purification.

Purification:

Article Title: Identification of genes and pathways associated with MDR in MCF-7/MDR breast cancer cells by RNA-seq analysis
Article Snippet: .. First-strand reverse transcription (RT) primer: biotin, 5′-CAAGCAGAAGACGGCATACGAGTVN-3′ was conducted using a SuperScript III First-Strand Synthesis system (Thermo Fisher Scientific, Inc.) ( ) was used to synthesize the first-strand cDNA according to the manufacturer's protocols, followed by purification of cDNA with a NucleoSpin gel and polymerase chain reaction (PCR) Clean-up kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) to remove free RT primer. .. Dynabeads® MyOne™ Streptavidin C1 (cat. no. 650.01; Thermo Fisher Scientific, Inc.) were used for further purification.

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    MACHEREY NAGEL pcr clean up kit
    Host and viral <t>DNA</t> content of differently treated ASFV samples. Quantitative dual <t>PCR</t> was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.
    Pcr Clean Up Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up kit/product/MACHEREY NAGEL
    Average 94 stars, based on 861 article reviews
    Price from $9.99 to $1999.99
    pcr clean up kit - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    90
    MACHEREY NAGEL nucleospin gdna clean up xs kit
    Removal of the inhibitory effects of melanin on PCR amplification. (A) Increasing concentrations of synthetic melanin were added to DNA extracted from 1676 melanoma cell lines. (B) Increasing concentrations of BSA (ng/μl) were added to DNA extracted from cultured cells containing 40 or 80 ng/μl of melanin. (C) The effect of diluting DNA assessed in the presence of 40 ng/μl of melanin and either 1U or 2U of Taq polymerase. (D) <t>NucleoSpin</t> ® <t>gDNA</t> Clean-up XS Kit used on DNA extracted from cultured cells containing 40 or 80 ng/μl of melanin. PCR amplification of the DNA was monitored on 2% gel agarose electrophoresis with ethidium bromide staining. MW, molecular weight markers.
    Nucleospin Gdna Clean Up Xs Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleospin gdna clean up xs kit/product/MACHEREY NAGEL
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nucleospin gdna clean up xs kit - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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    Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

    Journal: Viruses

    Article Title: A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus

    doi: 10.3390/v11121129

    Figure Lengend Snippet: Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

    Article Snippet: Amplified DNA Clean Up REPLI-g samples were purified using the NucleoSpin Gel and PCR clean-up Kit (Macherey-Nagel Düren, Germany).

    Techniques: Polymerase Chain Reaction, Whole Genome Amplification

    Recovery of three distinct coat-colour mutations by CRISPR/Cas. ( a ) Schematic illustration of three coat-colour mutations in rats. albino ( Tyr c ): SNP missense mutation in the Tyr gene. non-agouti ( Asip a ): 19-bp deletion in exon 2 of the Agouti signalling protein ( Asip ) gene. hooded ( Kit h ): integration of an 7,098-bp endogenous retrovirus (ERV) element within the first intron of the Kit gene. ( b ) Coat-colour phenotypes ( C, a, h ) recovered from albino by injecting gRNA: Tyr c , Cas9 mRNA, and ssODN of the Tyr C allele into F344 rat embryos ( c, a, h ). ( c ) Sequence analysis of the targeted Tyr exon 2 in the injected F344 rats. Cas9 and gRNA with ssODN mediated the introduction of several indel mutations, and the precise HDR-mediated SNP exchange of Tyr C . ( d ) Recovery of the non-agouti phenotype by injecting gRNA: Asip a , Cas9 mRNA, and ssODN of the Asip A allele into F344 rat embryos. Cas9 and gRNA with ssODN mediated the introduction of several indel mutations, and the precise short DNA fragment integration of the Asip A gene . ( e ) Recovery of the hooded mutation by injecting gRNA: Kit h -1 , gRNA: Kit h -2 , and ssODN of the Kit H allele into F344 rat embryos. Cas9 and two gRNAs with ssODN mediated the introduction of indel mutations at the targeted Kit h -1 locus, and the precise large deletion between the two cutting edges of Kit H . ( f ) PCR analysis of the injected F344 rats using primers designed against each outer side of the two LTR sequences. M: DNA marker phiX174-HaeIII digest.

    Journal: Nature Communications

    Article Title: Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform

    doi: 10.1038/ncomms5240

    Figure Lengend Snippet: Recovery of three distinct coat-colour mutations by CRISPR/Cas. ( a ) Schematic illustration of three coat-colour mutations in rats. albino ( Tyr c ): SNP missense mutation in the Tyr gene. non-agouti ( Asip a ): 19-bp deletion in exon 2 of the Agouti signalling protein ( Asip ) gene. hooded ( Kit h ): integration of an 7,098-bp endogenous retrovirus (ERV) element within the first intron of the Kit gene. ( b ) Coat-colour phenotypes ( C, a, h ) recovered from albino by injecting gRNA: Tyr c , Cas9 mRNA, and ssODN of the Tyr C allele into F344 rat embryos ( c, a, h ). ( c ) Sequence analysis of the targeted Tyr exon 2 in the injected F344 rats. Cas9 and gRNA with ssODN mediated the introduction of several indel mutations, and the precise HDR-mediated SNP exchange of Tyr C . ( d ) Recovery of the non-agouti phenotype by injecting gRNA: Asip a , Cas9 mRNA, and ssODN of the Asip A allele into F344 rat embryos. Cas9 and gRNA with ssODN mediated the introduction of several indel mutations, and the precise short DNA fragment integration of the Asip A gene . ( e ) Recovery of the hooded mutation by injecting gRNA: Kit h -1 , gRNA: Kit h -2 , and ssODN of the Kit H allele into F344 rat embryos. Cas9 and two gRNAs with ssODN mediated the introduction of indel mutations at the targeted Kit h -1 locus, and the precise large deletion between the two cutting edges of Kit H . ( f ) PCR analysis of the injected F344 rats using primers designed against each outer side of the two LTR sequences. M: DNA marker phiX174-HaeIII digest.

    Article Snippet: To prepare Cas9 mRNA and gRNA, T7-promoter Cas9 and gRNA expression plasmids were linearized with Xho I and Hind III, respectively, and extracted with NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel, Düren, Germany).

    Techniques: CRISPR, Mutagenesis, Sequencing, Injection, Polymerase Chain Reaction, Marker

    Relative transcript levels of QQ-ORFs in A . aurita polyps and ephyrae. Relative transcript levels of aaqq1 , aaqq2 and aaqq3 were determined by qRT-PCR analysis in A . aurita polyps and ephyrae with at least three independent biological experiments, each with three technical replicates. ( A ) Polyps and ephyrae were kept under native and germ-free conditions. ( B–E ) Polyps and ephyrae were kept under native ( B+D ) and germ-free ( C+E ) conditions, additionally challenged with selected bacteria, K . oxytoca , V . parahaemolyticus and P . tunicata . Fold changes in transcript abundances were determined by comparison with the threshold cycle (Ct) of transcripts of the control gene actin .

    Journal: Scientific Reports

    Article Title: Potential role of host-derived quorum quenching in modulating bacterial colonization in the moon jellyfish Aurelia aurita

    doi: 10.1038/s41598-018-37321-z

    Figure Lengend Snippet: Relative transcript levels of QQ-ORFs in A . aurita polyps and ephyrae. Relative transcript levels of aaqq1 , aaqq2 and aaqq3 were determined by qRT-PCR analysis in A . aurita polyps and ephyrae with at least three independent biological experiments, each with three technical replicates. ( A ) Polyps and ephyrae were kept under native and germ-free conditions. ( B–E ) Polyps and ephyrae were kept under native ( B+D ) and germ-free ( C+E ) conditions, additionally challenged with selected bacteria, K . oxytoca , V . parahaemolyticus and P . tunicata . Fold changes in transcript abundances were determined by comparison with the threshold cycle (Ct) of transcripts of the control gene actin .

    Article Snippet: Before use of cDNA in quantitative PCR, samples were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). qRT-PCRs were performed with three independent RNA preparations each with three technical replicates on an Applied Biosystems 7300 Real-Time PCR System using cDNA prepared with PlatinumR SYBRR Green qPCR SuperMix-UDG with ROX (Thermo Fisher Scientific, Darmstadt, Germany).

    Techniques: Quantitative RT-PCR

    Characterization of A . aurita QQ ORFs. ( A ) Arrangements of predicted ORFs on original metagenomic fosmid inserts (supplemental dataset) are depicted, carrying the genes aaqq1 , aaqq2 and aaqq3 linked to QQ phenotypes (black arrows, Accession Nos. JX274296-JX274298). ( B ) QQ-ORFs were cloned into pMAL-c2X and purified as MBP-fusion proteins by affinity chromatography. 5 µg of elution fractions were analyzed on 12% SDS-PAGE. Purified MBP-QQ proteins (0.1 µg, 1 µg and 10 µg) were tested regarding their QQ activities using reporter strains AI1-QQ.1 and AI2-QQ.1 (AHL, left panel; AI-2, right panel) monitoring QQ activities by growth. ( C ) QQ proteins were analyzed using secondary structure prediction software PredictProtein ( http://www.predictprotein.org/ ) with following visual output in Profsec: yellow, strand; red, helix. InterProScan ( http://www.ebi.ac.uk/Tools/pfa/iprscan/ ) and GlobPlot ( http://globplot.embl.de/ ) were used for functional protein analysis. ( D ) Fragments of QQ-ORFs were PCR-amplified from genomic DNA as well as cDNA transcribed from RNA of antibiotic-treated polyps (P) and ephyrae (E). C, non-template control. The presented gel grouping is cropped from different gels.

    Journal: Scientific Reports

    Article Title: Potential role of host-derived quorum quenching in modulating bacterial colonization in the moon jellyfish Aurelia aurita

    doi: 10.1038/s41598-018-37321-z

    Figure Lengend Snippet: Characterization of A . aurita QQ ORFs. ( A ) Arrangements of predicted ORFs on original metagenomic fosmid inserts (supplemental dataset) are depicted, carrying the genes aaqq1 , aaqq2 and aaqq3 linked to QQ phenotypes (black arrows, Accession Nos. JX274296-JX274298). ( B ) QQ-ORFs were cloned into pMAL-c2X and purified as MBP-fusion proteins by affinity chromatography. 5 µg of elution fractions were analyzed on 12% SDS-PAGE. Purified MBP-QQ proteins (0.1 µg, 1 µg and 10 µg) were tested regarding their QQ activities using reporter strains AI1-QQ.1 and AI2-QQ.1 (AHL, left panel; AI-2, right panel) monitoring QQ activities by growth. ( C ) QQ proteins were analyzed using secondary structure prediction software PredictProtein ( http://www.predictprotein.org/ ) with following visual output in Profsec: yellow, strand; red, helix. InterProScan ( http://www.ebi.ac.uk/Tools/pfa/iprscan/ ) and GlobPlot ( http://globplot.embl.de/ ) were used for functional protein analysis. ( D ) Fragments of QQ-ORFs were PCR-amplified from genomic DNA as well as cDNA transcribed from RNA of antibiotic-treated polyps (P) and ephyrae (E). C, non-template control. The presented gel grouping is cropped from different gels.

    Article Snippet: Before use of cDNA in quantitative PCR, samples were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). qRT-PCRs were performed with three independent RNA preparations each with three technical replicates on an Applied Biosystems 7300 Real-Time PCR System using cDNA prepared with PlatinumR SYBRR Green qPCR SuperMix-UDG with ROX (Thermo Fisher Scientific, Darmstadt, Germany).

    Techniques: Clone Assay, Purification, Affinity Chromatography, SDS Page, Software, Functional Assay, Polymerase Chain Reaction, Amplification

    Removal of the inhibitory effects of melanin on PCR amplification. (A) Increasing concentrations of synthetic melanin were added to DNA extracted from 1676 melanoma cell lines. (B) Increasing concentrations of BSA (ng/μl) were added to DNA extracted from cultured cells containing 40 or 80 ng/μl of melanin. (C) The effect of diluting DNA assessed in the presence of 40 ng/μl of melanin and either 1U or 2U of Taq polymerase. (D) NucleoSpin ® gDNA Clean-up XS Kit used on DNA extracted from cultured cells containing 40 or 80 ng/μl of melanin. PCR amplification of the DNA was monitored on 2% gel agarose electrophoresis with ethidium bromide staining. MW, molecular weight markers.

    Journal: PLoS ONE

    Article Title: Comparative Methods to Improve the Detection of BRAF V600 Mutations in Highly Pigmented Melanoma Specimens

    doi: 10.1371/journal.pone.0158698

    Figure Lengend Snippet: Removal of the inhibitory effects of melanin on PCR amplification. (A) Increasing concentrations of synthetic melanin were added to DNA extracted from 1676 melanoma cell lines. (B) Increasing concentrations of BSA (ng/μl) were added to DNA extracted from cultured cells containing 40 or 80 ng/μl of melanin. (C) The effect of diluting DNA assessed in the presence of 40 ng/μl of melanin and either 1U or 2U of Taq polymerase. (D) NucleoSpin ® gDNA Clean-up XS Kit used on DNA extracted from cultured cells containing 40 or 80 ng/μl of melanin. PCR amplification of the DNA was monitored on 2% gel agarose electrophoresis with ethidium bromide staining. MW, molecular weight markers.

    Article Snippet: Finally, the NucleoSpin® gDNA Clean-up XS Kit provided greater efficiency of BRAF amplification but did not completely resolve the difficulties presented by these types of samples, notably when higher concentrations of melanin were added ( ).

    Techniques: Polymerase Chain Reaction, Amplification, Cell Culture, Electrophoresis, Staining, Molecular Weight