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Promega polymerase chain reaction pcr buffer
Polymerase Chain Reaction Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/Promega
Average 89 stars, based on 8 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
89/100 stars

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Polymerase Chain Reaction:

Article Title: Suppression of immune responses in pigs by nonstructural protein 1 of Porcine reproductive and respiratory syndrome virus
Article Snippet: .. Amplification was performed in a 50-μL reaction mixture containing 1.5 mM of MgCl2 , 1× polymerase chain reaction (PCR) buffer, 0.2 mM of each dNTP, 20 pM of each primer, 1.5 U of Taq DNA polymerase (Promega, Madison, Wisconsin, USA), and 2 μL of complementary DNA. .. A PTC-150 thermocycler (MJ Research, South San Francisco, California, USA) was used with the following program: denaturation at 94°C for 5 min, 30 cycles composed of denaturation at 94°C for 40 s, annealing at 60°C for 40 s, and extension at 72°C for 1 min, and a final extension at 72°C for 10 min.

Article Title: Molecular Analysis of Phyllodes Tumors Reveals Distinct Changes in the Epithelial and Stromal Components
Article Snippet: .. For smaller amounts of tissue, generally the epithelium, DNA extraction was performed by incubating at 55°C in 1× polymerase chain reaction (PCR) buffer (Promega, Madison, WI) at pH 8 with proteinase K at 200 μg/ml overnight. ..

Article Title: Generation of mice by transplantation of an adult spermatogonial cell line after cryopreservation
Article Snippet: .. A 25‐µl reaction system consisted of 50–100 ng genomic DNA, 2.5 µl 10× polymerase chain reaction (PCR) buffer (15 m m MgCl2 ), 2% formamide, 0.2 m m dNTP, 1 µ m primer mix (0.5 µ m each), and 1 U Taq polymerase (Promega, Madison, WI, USA). .. PCRs were performed as follows: 94 °C for 4 min (94 °C for 30 s, 41 °C for 45 s, 72 °C for 2 min) × 30 cycles, and 72 °C for 7 min, executed in PerkinElmer Model 9600 DNA thermal cycler (PerkinElmer Cetus Instruments, Norwalk, CT, USA).

Article Title: An Integrated Characterization of Jujube (Ziziphus jujuba Mill.) Grown in the North Adriatic Region
Article Snippet: .. The 15 µL reaction volume contained 1× supplied polymerase chain reaction (PCR) buffer (Promega, Manheim, Germany), 2 mM MgCl2 (Promega), 1 mM deoxynucleotide triphosphate (dNTP) solution mix (Sigma-Aldrich, Merck, St. Louis, MO, USA), 0.2 µM of forward and reverse primers (IDT-DNA, Leuven, Belgium) where one of the primers was elongated with the M13(-22) tail at the 5’ end , 0.25 µM of M13 (-22) universal primer labelled with the G5 fluorescent dye set (Applied Biosystems, Woolston, UK), 1.25 unit of Taq (thermostable DNA) polymerase (Promega), and 40 ng of jujube DNA. .. Microsatellite amplification was performed in a Thermal Cycler 2720 (Applied Biosystems, Thermo Fisher Scientific, Singapore).

Article Title: Leukotoxicity of Aggregatibacter actinomycetemcomitansin generalized aggressive periodontitis in Brazilians and their family members
Article Snippet: .. The bacterial cells or suspension were dispersed by vortexing at maximal setting for 1 min and centrifuged at 12,000x g for 10 min. Genomic DNA was extracted from the pellet (InstaGene Matrix, Bio-Rad Laboratories, Hercules, CA, USA) and 20 µL aliquot of the resultant supernatant was added to 30 µL of the reaction mixture containing 25 µM polymerase chain reaction (PCR) buffer (Promega Corporation, Madison, WI, USA), 25 µM MgCl2 (Promega Corporation, Madison, WI, USA), 0.2 µM dNTP mix (Promega Corporation, Madison, WI, USA), 1.25 U Taq polymerase (Promega Corporation, Madison, WI, USA), and 100 ng of each primer (Invitrogen, São Paulo, SP, Brazil), resulting in a final volume of 50 µL. .. First, PCR with specific primers (5'-G G A C T A Y A G G G T A T C T A A T - 3'; 5'-AGAGTTTGATCMTGG-3') for the 16S ribosomal DNA (16S rDNA) was performed to confirm the presence of bacterial DNA .

Article Title: Gastrin-Releasing Peptide Is a Growth Factor for Human Neuroblastomas
Article Snippet: .. Polymerase chain reaction (PCR) buffer (10×) and Taq polymerase were from Promega (Madison, WI). .. GRP-R PCR primers were synthesized by Oligos Etc.

Article Title: The spectrum of p53 mutations in colorectal adenomas differs from that in colorectal carcinomas
Article Snippet: .. The microdissected cell populations were digested in 100 μl of 1× polymerase chain reaction (PCR) buffer without magnesium (Promega Corporation, Madison, Wisconsin, USA) containing 200 mg/l proteinase K, for 24–48 hours at 55°C. .. Proteinase K was thereafter inactivated by heating to 96°C for 10 minutes, the cell debris pelleted by centrifugation (12 000 g for five minutes), and the supernatant used for PCR.

DNA Extraction:

Article Title: Molecular Analysis of Phyllodes Tumors Reveals Distinct Changes in the Epithelial and Stromal Components
Article Snippet: .. For smaller amounts of tissue, generally the epithelium, DNA extraction was performed by incubating at 55°C in 1× polymerase chain reaction (PCR) buffer (Promega, Madison, WI) at pH 8 with proteinase K at 200 μg/ml overnight. ..

Amplification:

Article Title: Suppression of immune responses in pigs by nonstructural protein 1 of Porcine reproductive and respiratory syndrome virus
Article Snippet: .. Amplification was performed in a 50-μL reaction mixture containing 1.5 mM of MgCl2 , 1× polymerase chain reaction (PCR) buffer, 0.2 mM of each dNTP, 20 pM of each primer, 1.5 U of Taq DNA polymerase (Promega, Madison, Wisconsin, USA), and 2 μL of complementary DNA. .. A PTC-150 thermocycler (MJ Research, South San Francisco, California, USA) was used with the following program: denaturation at 94°C for 5 min, 30 cycles composed of denaturation at 94°C for 40 s, annealing at 60°C for 40 s, and extension at 72°C for 1 min, and a final extension at 72°C for 10 min.

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  • 94
    Promega pcr buffer
    Optimization of annealing temperature and Mg 2+ concentration for <t>PCR</t> with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target <t>DNA</t> for PCR was extracted from C. pneumoniae with the QIAmp DNA Mini Kit.
    Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 1066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Promega
    Average 94 stars, based on 1066 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    85
    Promega flexi ligase buffer
    ORF transfer in the <t>Flexi</t> ® Vector cloning system. ( A ) Flanking sequences of ORF in Flexi clones. Recognition sequences of Sgf I and <t>Pme</t> I are indicated as green and red characters, respectively. The nucleotide sequence corresponding to the ribosomal binding site is underlined. The amino acid sequence encoded in the frame in the flanking regions of the ORF is indicated as a three-letter code. Recognition sequences of Bst BI and Sna BI, arising in the vector of Flexi_RBS type are indicated as blue characters. ( B ) Transfer of the ORF from the pF1K clone to multiple expression vectors. The ORF sequence in the pF1K clone can be easily transferred to a variety of other expression vectors with the correct orientation after digestion by Sgf I and Pme I. For construction of a C-terminal tag-fusion clone, Sgf I– Pme I ORF sequence must be cloned into Sgf I and Eco ICRI sites of the expression vector to omit a stop codon arising in the Pme I site. The appropriate promoter is indicated as an orange arrow in the vectors.
    Flexi Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flexi ligase buffer/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flexi ligase buffer - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    89
    Promega amplitaq gold buffer
    T1942C PCR-SSCP. Three DNA samples, 1942T/T (homozygous normal), 1942C/C (homozygous polymorphic), 1942T/C (heterozygous), were amplified with <t>AmpliTaq</t> Gold polymerase and AmpliTaq Gold buffer ( A ), Promega Taq and Promega buffer ( B ), Promega Taq and AmpliTaq Gold buffer ( C ) and with AmpliTaq Gold and Promega buffer (no PCR products obtained). All samples were amplified and SSCP-electrophoresed in the same experiment. Arrows point to the polymorphic SSCP fragment. Note that the polymorphic fragments are clearly visible only after amplification in AmpliTaq Gold buffer ( A , C ).
    Amplitaq Gold Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold buffer/product/Promega
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold buffer - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

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    Optimization of annealing temperature and Mg 2+ concentration for PCR with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target DNA for PCR was extracted from C. pneumoniae with the QIAmp DNA Mini Kit.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Optimization of annealing temperature and Mg 2+ concentration for PCR with primers for omp1 . The annealing temperature was optimized in a Mastercycler gradient (Eppendorf). The Mg concentration for PCR specific for omp1 was optimized with a PCR optimization kit (Invitrogen). The target DNA for PCR was extracted from C. pneumoniae with the QIAmp DNA Mini Kit.

    Article Snippet: In brief, 2 μl of DNA extracts was processed in a 25-μl reaction volume containing PCR buffer (10 mM Tris [pH 9.0], 50 mM KCl, 0.01% gelatin), 200 μM deoxynucleoside triphosphates, 3.5 mM MgCl2 , 0.5 μM each primer, and 1 U of Taq polymerase (Promega, Madison, Wis.).

    Techniques: Concentration Assay, Polymerase Chain Reaction

    Detection sensitivity of PCR for omp1 versus the 16S rRNA gene. Two hundred microliters of the mock CSF containing a specific number of C. pneumoniae organisms (as indicated), was used for DNA extraction with the QIAmp DNA Mini Kit. A 2-μl portion of the 50-μl volume of DNA extracts was subjected to PCR. The optimized PCRs for omp1 and 16S rRNA gene were conducted (see Materials and Methods).

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Detection sensitivity of PCR for omp1 versus the 16S rRNA gene. Two hundred microliters of the mock CSF containing a specific number of C. pneumoniae organisms (as indicated), was used for DNA extraction with the QIAmp DNA Mini Kit. A 2-μl portion of the 50-μl volume of DNA extracts was subjected to PCR. The optimized PCRs for omp1 and 16S rRNA gene were conducted (see Materials and Methods).

    Article Snippet: In brief, 2 μl of DNA extracts was processed in a 25-μl reaction volume containing PCR buffer (10 mM Tris [pH 9.0], 50 mM KCl, 0.01% gelatin), 200 μM deoxynucleoside triphosphates, 3.5 mM MgCl2 , 0.5 μM each primer, and 1 U of Taq polymerase (Promega, Madison, Wis.).

    Techniques: Polymerase Chain Reaction, DNA Extraction

    Comparison of extraction efficacy of C. pneumoniae DNA from mock CSFs using two DNA extraction kits. The mock CSFs (200 μl) spiked with serially diluted bacteria were extracted either with the QIAmp DNA Mini Kit with a bacterial DNA extraction protocol or with the QIAmp DNA Blood Mini Kit. Two microliters of the extracted DNA (50 μl) was subjected to PCR with primers for omp1 . Results are representative of three experiments.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Comparison of extraction efficacy of C. pneumoniae DNA from mock CSFs using two DNA extraction kits. The mock CSFs (200 μl) spiked with serially diluted bacteria were extracted either with the QIAmp DNA Mini Kit with a bacterial DNA extraction protocol or with the QIAmp DNA Blood Mini Kit. Two microliters of the extracted DNA (50 μl) was subjected to PCR with primers for omp1 . Results are representative of three experiments.

    Article Snippet: In brief, 2 μl of DNA extracts was processed in a 25-μl reaction volume containing PCR buffer (10 mM Tris [pH 9.0], 50 mM KCl, 0.01% gelatin), 200 μM deoxynucleoside triphosphates, 3.5 mM MgCl2 , 0.5 μM each primer, and 1 U of Taq polymerase (Promega, Madison, Wis.).

    Techniques: DNA Extraction, Polymerase Chain Reaction

    Detection of C. pneumoniae DNA by PCR in CSFs obtained from patients with MS. Two hundred microliters of CSFs obtained from MS patients was processed for extraction of DNA utilizing the QIAmp DNA Mini Kit, and 2 μl of the resulting 50-μl DNA solution was subjected to PCR with primers for either omp1 or the 16S rRNA gene. Data are representative of three PCR experiments. Boxed numbers indicate CSFs obtained from the same patient at different time points. Each number is the sample number. M, molecular marker; PC, positive control for PCR; NC, negative control for PCR; IC, negative control for DNA extraction.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: PCR-Based Method for Isolation and Detection of Chlamydia pneumoniae DNA in Cerebrospinal Fluids

    doi: 10.1128/CDLI.8.3.499-502.2001

    Figure Lengend Snippet: Detection of C. pneumoniae DNA by PCR in CSFs obtained from patients with MS. Two hundred microliters of CSFs obtained from MS patients was processed for extraction of DNA utilizing the QIAmp DNA Mini Kit, and 2 μl of the resulting 50-μl DNA solution was subjected to PCR with primers for either omp1 or the 16S rRNA gene. Data are representative of three PCR experiments. Boxed numbers indicate CSFs obtained from the same patient at different time points. Each number is the sample number. M, molecular marker; PC, positive control for PCR; NC, negative control for PCR; IC, negative control for DNA extraction.

    Article Snippet: In brief, 2 μl of DNA extracts was processed in a 25-μl reaction volume containing PCR buffer (10 mM Tris [pH 9.0], 50 mM KCl, 0.01% gelatin), 200 μM deoxynucleoside triphosphates, 3.5 mM MgCl2 , 0.5 μM each primer, and 1 U of Taq polymerase (Promega, Madison, Wis.).

    Techniques: Polymerase Chain Reaction, Mass Spectrometry, Marker, Positive Control, Negative Control, DNA Extraction

    Amplification of the VP7 and VP4 genes by RT-PCR. Amplification products corresponding to the VP7 (lanes 2, 3, and 4) and VP4 genes (lanes 5, 6, and 7) in three different patient samples are shown. Lane 1, negative control; lane M, molecular weight markers (100-bp ladder). The sizes of amplified bands are indicated.

    Journal: Journal of Clinical Microbiology

    Article Title: VP7 and VP4 Genotyping of Human Group A Rotavirus in Buenos Aires, Argentina

    doi:

    Figure Lengend Snippet: Amplification of the VP7 and VP4 genes by RT-PCR. Amplification products corresponding to the VP7 (lanes 2, 3, and 4) and VP4 genes (lanes 5, 6, and 7) in three different patient samples are shown. Lane 1, negative control; lane M, molecular weight markers (100-bp ladder). The sizes of amplified bands are indicated.

    Article Snippet: The reaction volume was brought to 10 μl by adding the PCR mixture, which contained 0.25 μM concentrations of primers beg and end (VP7) and 1 and 2 (VP4), 1 μl of the PCR buffer supplied with the enzyme, 0.75 U of Taq DNA polymerase B (Promega), 1 μg of bovine serum albumin (Sigma Chemical Co.), 100 μM concentrations of each of the deoxynucleoside triphosphates, and 2 μM MgCl2 .

    Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Negative Control, Molecular Weight

    Detection of virus and coproantibodies in consecutive samples. (A) Consecutive samples from two patients taken at daily intervals and tested for rotavirus antigens (●) and rotavirus IgM (▵) and IgA (▴) antibodies. —, RT-PCR-positive samples. The arrow indicates illness onset. (B) Amplification products obtained by RT-PCR with VP4-specific primers (876 bp) in consecutive samples. Lane 0, disease onset; lanes 1 to 5, 1 to 5 days after onset, respectively; lane M, molecular weight markers (100-bp ladder).

    Journal: Journal of Clinical Microbiology

    Article Title: VP7 and VP4 Genotyping of Human Group A Rotavirus in Buenos Aires, Argentina

    doi:

    Figure Lengend Snippet: Detection of virus and coproantibodies in consecutive samples. (A) Consecutive samples from two patients taken at daily intervals and tested for rotavirus antigens (●) and rotavirus IgM (▵) and IgA (▴) antibodies. —, RT-PCR-positive samples. The arrow indicates illness onset. (B) Amplification products obtained by RT-PCR with VP4-specific primers (876 bp) in consecutive samples. Lane 0, disease onset; lanes 1 to 5, 1 to 5 days after onset, respectively; lane M, molecular weight markers (100-bp ladder).

    Article Snippet: The reaction volume was brought to 10 μl by adding the PCR mixture, which contained 0.25 μM concentrations of primers beg and end (VP7) and 1 and 2 (VP4), 1 μl of the PCR buffer supplied with the enzyme, 0.75 U of Taq DNA polymerase B (Promega), 1 μg of bovine serum albumin (Sigma Chemical Co.), 100 μM concentrations of each of the deoxynucleoside triphosphates, and 2 μM MgCl2 .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Molecular Weight

    ORF transfer in the Flexi ® Vector cloning system. ( A ) Flanking sequences of ORF in Flexi clones. Recognition sequences of Sgf I and Pme I are indicated as green and red characters, respectively. The nucleotide sequence corresponding to the ribosomal binding site is underlined. The amino acid sequence encoded in the frame in the flanking regions of the ORF is indicated as a three-letter code. Recognition sequences of Bst BI and Sna BI, arising in the vector of Flexi_RBS type are indicated as blue characters. ( B ) Transfer of the ORF from the pF1K clone to multiple expression vectors. The ORF sequence in the pF1K clone can be easily transferred to a variety of other expression vectors with the correct orientation after digestion by Sgf I and Pme I. For construction of a C-terminal tag-fusion clone, Sgf I– Pme I ORF sequence must be cloned into Sgf I and Eco ICRI sites of the expression vector to omit a stop codon arising in the Pme I site. The appropriate promoter is indicated as an orange arrow in the vectors.

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: Exploration of Human ORFeome: High-Throughput Preparation of ORF Clones and Efficient Characterization of Their Protein Products

    doi: 10.1093/dnares/dsn004

    Figure Lengend Snippet: ORF transfer in the Flexi ® Vector cloning system. ( A ) Flanking sequences of ORF in Flexi clones. Recognition sequences of Sgf I and Pme I are indicated as green and red characters, respectively. The nucleotide sequence corresponding to the ribosomal binding site is underlined. The amino acid sequence encoded in the frame in the flanking regions of the ORF is indicated as a three-letter code. Recognition sequences of Bst BI and Sna BI, arising in the vector of Flexi_RBS type are indicated as blue characters. ( B ) Transfer of the ORF from the pF1K clone to multiple expression vectors. The ORF sequence in the pF1K clone can be easily transferred to a variety of other expression vectors with the correct orientation after digestion by Sgf I and Pme I. For construction of a C-terminal tag-fusion clone, Sgf I– Pme I ORF sequence must be cloned into Sgf I and Eco ICRI sites of the expression vector to omit a stop codon arising in the Pme I site. The appropriate promoter is indicated as an orange arrow in the vectors.

    Article Snippet: After inactivation of the restriction enzymes by incubation for 20 min at 65°C, one-sixth of the digested PCR product was ligated with 25 ng of the Sgf I- and Pme I-digested pF1K T7 vector in a 10‐μL reaction of Flexi® Ligase buffer with 10 units of T4 DNA Ligase (Promega) for 60 min at 25°C.

    Techniques: Plasmid Preparation, Clone Assay, Sequencing, Binding Assay, Expressing

    T1942C PCR-SSCP. Three DNA samples, 1942T/T (homozygous normal), 1942C/C (homozygous polymorphic), 1942T/C (heterozygous), were amplified with AmpliTaq Gold polymerase and AmpliTaq Gold buffer ( A ), Promega Taq and Promega buffer ( B ), Promega Taq and AmpliTaq Gold buffer ( C ) and with AmpliTaq Gold and Promega buffer (no PCR products obtained). All samples were amplified and SSCP-electrophoresed in the same experiment. Arrows point to the polymorphic SSCP fragment. Note that the polymorphic fragments are clearly visible only after amplification in AmpliTaq Gold buffer ( A , C ).

    Journal: British Journal of Cancer

    Article Title: Frequent loss of the AXIN1 locus but absence of AXIN1 gene mutations in adenocarcinomas of the gastro-oesophageal junction with nuclear β-catenin expression

    doi: 10.1038/sj.bjc.6601589

    Figure Lengend Snippet: T1942C PCR-SSCP. Three DNA samples, 1942T/T (homozygous normal), 1942C/C (homozygous polymorphic), 1942T/C (heterozygous), were amplified with AmpliTaq Gold polymerase and AmpliTaq Gold buffer ( A ), Promega Taq and Promega buffer ( B ), Promega Taq and AmpliTaq Gold buffer ( C ) and with AmpliTaq Gold and Promega buffer (no PCR products obtained). All samples were amplified and SSCP-electrophoresed in the same experiment. Arrows point to the polymorphic SSCP fragment. Note that the polymorphic fragments are clearly visible only after amplification in AmpliTaq Gold buffer ( A , C ).

    Article Snippet: The AmpliTaq Gold buffer consists of 15 m Tris-HCl (pH 8.0), 50 m KCl and 1.5 m MgCl2 and the Promega buffer consists of 10 m Tris–HCl (pH 9.0), 50 m KCl, 1.5 m MgCl2 and 0.1% Triton X-100.

    Techniques: Polymerase Chain Reaction, Amplification