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GE Healthcare polymerase chain reaction pcr buffer
Polymerase Chain Reaction Pcr Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/GE Healthcare
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr buffer - by Bioz Stars, 2020-08
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Polymerase Chain Reaction:

Article Title: Low Frequency of FAS Mutations in Reed-Sternberg Cells of Hodgkin's Lymphoma
Article Snippet: .. From each case single RS cells were microdissected and 30 cells were pooled per tube containing 30 μl of polymerase chain reaction (PCR) buffer (Amersham Pharmacia Biosciences, Roosendaal, The Netherlands). ..

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  • 92
    GE Healthcare pcr buffer
    RC1339/ APRc is expressed in Rickettsia conorii and Rickettsia rickettsii and accumulates at the outer membrane in both species. (A) <t>RT-PCR</t> analysis of RC1339/ APRc expression on rickettsial spp.. The housekeeping gene hrtA (17 kDa surface antigen) was used as a control. The negative control for the <t>cDNA</t> synthesis lacking reverse transcriptase is identified by (RTase -). Rickettsial species are identified on the top and the gene names are shown on the left side of the agarose gel. (B) A whole cell lysate from R. rickettsii (1) and insoluble (2) and soluble (3) fractions from R. conorii extracts were isolated and then subjected to Western Blot analysis with anti-APRc antibody. A specific band with approximately 21 kDa was detected. (C) Whole cell lysates (WCL), inner (IM) and outer membrane (OM) fractions from sarkosyl treatment of R. rickettsii and R. conorii extracts were isolated and then subjected to Western Blot analysis with anti-APRc and anti-rOmpB antibody. APRc shares the same localization of rOmpB, an internal marker for outer membrane of Rickettsia spp. Molecular mass markers in kilodaltons (kDa) are shown on the left. (D) For flow cytometric analysis of APRc expression in R. conorii , fixed bacteria were queried for deposition of anti-APRc (orange trace), negative control lacking primay antibody (blue trace), or the positive control anti-OmpB (red trace), a known rickettsial surface protein. After incubation with fluorescent secondary antibody, both anti-APRc and anti-OmpB detected on the bacterial surface (increased fluorescence), indicating accessibility of these target proteins to exogenously applied antibody.
    Pcr Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/GE Healthcare
    Average 92 stars, based on 135 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    GE Healthcare pcr solution
    Scheme of systematic enrichment of aptamers against PDAC PL45 cell line. The <t>DNA</t> library was incubated with hTERT-HPNE cells (negative cells) to remove negative cell-binding sequences. The unbound DNAs were collected and then incubated with target PL45 cell line for positive selection. After washing, the bound DNAs were eluted and amplified by <t>PCR</t> for next-round selection. After enrichment, DNA sequencing was performed to identify individual aptamer sequences.
    Pcr Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr solution/product/GE Healthcare
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pcr solution - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    GE Healthcare control pbs mice
    Liver histopathological analyses in <t>LPF</t> infected mice. Mice were inoculated i/p with 1.5 × 10 8 LPF or only <t>PBS</t> and then euthanized on the third or the sixth day post-infection ( n ≥ 5). (A) Liver sections (3–5 μm) were stained with (HE) and evaluated at 200x magnification. Arrowheads indicate leukocyte infiltrates in the portal space and in the hepatic sinusoids. Asterisks indicate mitotic cells. Inset: mitotic cells in larger magnification. (B) Total scores of hepatic lesions. The significant difference ( p
    Control Pbs Mice, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control pbs mice/product/GE Healthcare
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    control pbs mice - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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    RC1339/ APRc is expressed in Rickettsia conorii and Rickettsia rickettsii and accumulates at the outer membrane in both species. (A) RT-PCR analysis of RC1339/ APRc expression on rickettsial spp.. The housekeeping gene hrtA (17 kDa surface antigen) was used as a control. The negative control for the cDNA synthesis lacking reverse transcriptase is identified by (RTase -). Rickettsial species are identified on the top and the gene names are shown on the left side of the agarose gel. (B) A whole cell lysate from R. rickettsii (1) and insoluble (2) and soluble (3) fractions from R. conorii extracts were isolated and then subjected to Western Blot analysis with anti-APRc antibody. A specific band with approximately 21 kDa was detected. (C) Whole cell lysates (WCL), inner (IM) and outer membrane (OM) fractions from sarkosyl treatment of R. rickettsii and R. conorii extracts were isolated and then subjected to Western Blot analysis with anti-APRc and anti-rOmpB antibody. APRc shares the same localization of rOmpB, an internal marker for outer membrane of Rickettsia spp. Molecular mass markers in kilodaltons (kDa) are shown on the left. (D) For flow cytometric analysis of APRc expression in R. conorii , fixed bacteria were queried for deposition of anti-APRc (orange trace), negative control lacking primay antibody (blue trace), or the positive control anti-OmpB (red trace), a known rickettsial surface protein. After incubation with fluorescent secondary antibody, both anti-APRc and anti-OmpB detected on the bacterial surface (increased fluorescence), indicating accessibility of these target proteins to exogenously applied antibody.

    Journal: PLoS Pathogens

    Article Title: RC1339/APRc from Rickettsia conorii Is a Novel Aspartic Protease with Properties of Retropepsin-Like Enzymes

    doi: 10.1371/journal.ppat.1004324

    Figure Lengend Snippet: RC1339/ APRc is expressed in Rickettsia conorii and Rickettsia rickettsii and accumulates at the outer membrane in both species. (A) RT-PCR analysis of RC1339/ APRc expression on rickettsial spp.. The housekeeping gene hrtA (17 kDa surface antigen) was used as a control. The negative control for the cDNA synthesis lacking reverse transcriptase is identified by (RTase -). Rickettsial species are identified on the top and the gene names are shown on the left side of the agarose gel. (B) A whole cell lysate from R. rickettsii (1) and insoluble (2) and soluble (3) fractions from R. conorii extracts were isolated and then subjected to Western Blot analysis with anti-APRc antibody. A specific band with approximately 21 kDa was detected. (C) Whole cell lysates (WCL), inner (IM) and outer membrane (OM) fractions from sarkosyl treatment of R. rickettsii and R. conorii extracts were isolated and then subjected to Western Blot analysis with anti-APRc and anti-rOmpB antibody. APRc shares the same localization of rOmpB, an internal marker for outer membrane of Rickettsia spp. Molecular mass markers in kilodaltons (kDa) are shown on the left. (D) For flow cytometric analysis of APRc expression in R. conorii , fixed bacteria were queried for deposition of anti-APRc (orange trace), negative control lacking primay antibody (blue trace), or the positive control anti-OmpB (red trace), a known rickettsial surface protein. After incubation with fluorescent secondary antibody, both anti-APRc and anti-OmpB detected on the bacterial surface (increased fluorescence), indicating accessibility of these target proteins to exogenously applied antibody.

    Article Snippet: The PCRs were performed in a 50 µl volume, with 1 µl of cDNA as the DNA template, 0.1 µM of each primer, 1× PCR buffer (100 mM Tris-HCl (pH 9.0), 15 mM MgCl2, 500 mM KCl), 200 µM of dNTP mix, and 1 U of Taq DNA polymerase (GE Healthcare).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Negative Control, Agarose Gel Electrophoresis, Isolation, Western Blot, Marker, Flow Cytometry, Positive Control, Incubation, Fluorescence

    Scheme of systematic enrichment of aptamers against PDAC PL45 cell line. The DNA library was incubated with hTERT-HPNE cells (negative cells) to remove negative cell-binding sequences. The unbound DNAs were collected and then incubated with target PL45 cell line for positive selection. After washing, the bound DNAs were eluted and amplified by PCR for next-round selection. After enrichment, DNA sequencing was performed to identify individual aptamer sequences.

    Journal: Theranostics

    Article Title: DNA Aptamer Selected against Pancreatic Ductal Adenocarcinoma for in vivo Imaging and Clinical Tissue Recognition

    doi: 10.7150/thno.11938

    Figure Lengend Snippet: Scheme of systematic enrichment of aptamers against PDAC PL45 cell line. The DNA library was incubated with hTERT-HPNE cells (negative cells) to remove negative cell-binding sequences. The unbound DNAs were collected and then incubated with target PL45 cell line for positive selection. After washing, the bound DNAs were eluted and amplified by PCR for next-round selection. After enrichment, DNA sequencing was performed to identify individual aptamer sequences.

    Article Snippet: The double-stranded DNA (dsDNA) product was then separated from the PCR solution by streptavidin-coated sepharose beads (GE Healthcare).

    Techniques: Incubation, Binding Assay, Selection, Amplification, Polymerase Chain Reaction, DNA Sequencing

    Scheme of cell-SELEX for GCGR-expressing CHO-K1 cells (CHO-GCGR). The ssDNA library was incubated with Mock cells as negative selection to remove the cell-binding ssDNA. The unbound ssDNA was incubated with GCGR-expressing CHO-K1 cells (CHO-GCGR) for positive selection. After washing, the bound DNA was eluted and amplified by PCR for next-round selection. The evolved ssDNA pool was sequenced to identify the aptamer candidates in the last round of selection.

    Journal: Scientific Reports

    Article Title: Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX

    doi: 10.1038/s41598-017-05840-w

    Figure Lengend Snippet: Scheme of cell-SELEX for GCGR-expressing CHO-K1 cells (CHO-GCGR). The ssDNA library was incubated with Mock cells as negative selection to remove the cell-binding ssDNA. The unbound ssDNA was incubated with GCGR-expressing CHO-K1 cells (CHO-GCGR) for positive selection. After washing, the bound DNA was eluted and amplified by PCR for next-round selection. The evolved ssDNA pool was sequenced to identify the aptamer candidates in the last round of selection.

    Article Snippet: The double-stranded DNA (dsDNA) product in the PCR solution was separated by streptavidin-coated sepharose beads (GE Healthcare).

    Techniques: Expressing, Incubation, Selection, Binding Assay, Amplification, Polymerase Chain Reaction

    Liver histopathological analyses in LPF infected mice. Mice were inoculated i/p with 1.5 × 10 8 LPF or only PBS and then euthanized on the third or the sixth day post-infection ( n ≥ 5). (A) Liver sections (3–5 μm) were stained with (HE) and evaluated at 200x magnification. Arrowheads indicate leukocyte infiltrates in the portal space and in the hepatic sinusoids. Asterisks indicate mitotic cells. Inset: mitotic cells in larger magnification. (B) Total scores of hepatic lesions. The significant difference ( p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Role of Murine Complement Component C5 in Acute in Vivo Infection by Pathogenic Leptospira interrogans

    doi: 10.3389/fcimb.2018.00063

    Figure Lengend Snippet: Liver histopathological analyses in LPF infected mice. Mice were inoculated i/p with 1.5 × 10 8 LPF or only PBS and then euthanized on the third or the sixth day post-infection ( n ≥ 5). (A) Liver sections (3–5 μm) were stained with (HE) and evaluated at 200x magnification. Arrowheads indicate leukocyte infiltrates in the portal space and in the hepatic sinusoids. Asterisks indicate mitotic cells. Inset: mitotic cells in larger magnification. (B) Total scores of hepatic lesions. The significant difference ( p

    Article Snippet: DNA extraction from liver and Leptospira DNA quantification by qPCR Total DNA was extracted from 20 to 25 mg of liver from LPF-infected or control (PBS) mice using the Illustra Tissue & Cells Genomic Prep Mini Spin kit (GE Healthcare, Little Chalfont, Buckinghamshire, UK) following the manufacturer's instructions.

    Techniques: Infection, Mouse Assay, Staining

    Spleen alterations during LPF infection. Mice were inoculated i/p with 1.5 × 10 8 LPF or only PBS and then euthanized on the third or sixth day post-infection ( n ≥ 5). (A) Splenomegaly was expressed as percentage of spleen mass to the total body mass. (B) Score of morphological alterations. (C) Spleen sections stained with HE. (D) Immunochemical analysis using anti-leptospiral antibodies. Arrows indicate the presence of LPF antigen.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Role of Murine Complement Component C5 in Acute in Vivo Infection by Pathogenic Leptospira interrogans

    doi: 10.3389/fcimb.2018.00063

    Figure Lengend Snippet: Spleen alterations during LPF infection. Mice were inoculated i/p with 1.5 × 10 8 LPF or only PBS and then euthanized on the third or sixth day post-infection ( n ≥ 5). (A) Splenomegaly was expressed as percentage of spleen mass to the total body mass. (B) Score of morphological alterations. (C) Spleen sections stained with HE. (D) Immunochemical analysis using anti-leptospiral antibodies. Arrows indicate the presence of LPF antigen.

    Article Snippet: DNA extraction from liver and Leptospira DNA quantification by qPCR Total DNA was extracted from 20 to 25 mg of liver from LPF-infected or control (PBS) mice using the Illustra Tissue & Cells Genomic Prep Mini Spin kit (GE Healthcare, Little Chalfont, Buckinghamshire, UK) following the manufacturer's instructions.

    Techniques: Infection, Mouse Assay, Staining