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Bioneer Corporation polymerase chain reaction pcr buffer
Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
Polymerase Chain Reaction Pcr Buffer, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
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1) Product Images from "Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients"

Article Title: Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients

Journal: Osong Public Health and Research Perspectives

doi: 10.1016/j.phrp.2013.09.005

Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
Figure Legend Snippet: Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

Techniques Used: Amplification, Polymerase Chain Reaction, DNA Sequencing

Related Articles

Polymerase Chain Reaction:

Article Title: Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients
Article Snippet: .. The DNA amplification was performed using 15 μL of solution containing 10 ng of genomic DNA, 1 U of i-Max II DNA polymerase (iNtRON Biotechnology, Gyeonggi-do, Korea), 2.5 mM deoxyribonucleotide triphosphate mixture, 10× polymerase chain reaction (PCR) buffer, 10 pmol of each primer (Bioneer, Daejeon, Korea), 5× betaine (Sigma Aldrich, St. Louis, MO, USA), and distilled sterilized water. .. Each reaction mixture was run in the Gene Amp PCR system 9700 (Applied Biosystems).

Amplification:

Article Title: Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients
Article Snippet: .. The DNA amplification was performed using 15 μL of solution containing 10 ng of genomic DNA, 1 U of i-Max II DNA polymerase (iNtRON Biotechnology, Gyeonggi-do, Korea), 2.5 mM deoxyribonucleotide triphosphate mixture, 10× polymerase chain reaction (PCR) buffer, 10 pmol of each primer (Bioneer, Daejeon, Korea), 5× betaine (Sigma Aldrich, St. Louis, MO, USA), and distilled sterilized water. .. Each reaction mixture was run in the Gene Amp PCR system 9700 (Applied Biosystems).

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    Bioneer Corporation polymerase chain reaction pcr buffer
    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated <t>PCR</t> products size of the reference strain was confirmed by <t>DNA</t> sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.
    Polymerase Chain Reaction Pcr Buffer, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr buffer/product/Bioneer Corporation
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr buffer - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    85
    Bioneer Corporation media original schizosaccharomyces pombe strains
    ( A ) Schematic representation of  S. pombe  chromosome ends and telomeric transcriptome. Telomeric repeats are in black and subtelomeric sequences in white. Oligonucleotides used for RT–PCR and/or northern blotting are indicated by arrows. The sketch is not in scale. ( B ) RNA isolated from wt and Δ rap1  cells was hybridized with the indicated probes. Exposures and signal detection were performed in parallel for TERRA and ARIA as well as for ARRET and αARRET. The black arrowheads indicate two discrete bands of ∼0.7 and 1.1 kb detected by oC and oG oligonucleotides and of so far unknown origin. The asterisks indicate major hybridization bands corresponding to ARRET and αARRET RNA species. U6 snRNA was used as loading control. Molecular weights are on the left in kb. ( C ) Total RNA from wt and Δ rap1  strains was reverse transcribed (RT) using oC (left panels) or o1 (right panels) oligonucleotides and cDNA was PCR amplified using o2+o3 or o2+oC oligonucleotides. The most right samples correspond to control reactions where template was omitted. Molecular weights are on the right in bp.
    Media Original Schizosaccharomyces Pombe Strains, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/media original schizosaccharomyces pombe strains/product/Bioneer Corporation
    Average 85 stars, based on 3 article reviews
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    93
    Bioneer Corporation pcr reaction buffer
    <t>DNA</t> marker paradox derived from MTPTs. ( a) Amplicons of a Ca-specific marker (Ca_s_1) designed from the matK region and its counterpart MTPTs in C. wilfordii (Cw) and C. auriculatum (Ca). Pt, plastid genome; mt, MTPT segment in the mitochondrial genome. Primer regions and target SNPs used for authentication of Cw and Ca are marked with red arrows and red stars, respectively. (b) Electrophoresis of <t>PCR</t> products from three authentication markers for Cynanchum species after different numbers of PCR cycles. Ca_s_1 is a Ca dominant marker, and Cw_i_1 and Cw_i_2 are co-dominant markers. Two accessions each of Cw and Ca were used. (c) Results from electrophoresis of PCR products obtained from Cw and Ca samples using the Ca-specific marker Ca_s_1 with different amounts of template DNA and different numbers of PCR cycles. MTPT-derived PCR products are denoted by white and yellow arrowheads in Cw and Ca, respectively.
    Pcr Reaction Buffer, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr reaction buffer/product/Bioneer Corporation
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pcr reaction buffer - by Bioz Stars, 2020-07
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    88
    Bioneer Corporation dna staining buffer
    Agarose gel electrophoresis of the amplified HIV gag gene (148 bp) prepared by a general <t>gDNA</t> extraction and on-chip process. The integrated chip could successfully extract the HIV proviral <t>DNA</t> from infected cell populations as low as 10 2 /μl in the blood sample. Lanes: M, molecular weight standard; C, PCR product by chemical method; IT, PCR product by IT chip with respect to sample population; N, negative control.
    Dna Staining Buffer, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna staining buffer/product/Bioneer Corporation
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna staining buffer - by Bioz Stars, 2020-07
    88/100 stars
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    Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

    Journal: Osong Public Health and Research Perspectives

    Article Title: Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients

    doi: 10.1016/j.phrp.2013.09.005

    Figure Lengend Snippet: Amplification patterns of MLVA on Brucella abortus biovar 1. Lane M, 100-bp ladder; Lane P, B. abortus 2308; Lanes 1–20, B. abortus biovar 1 isolates. The indicated PCR products size of the reference strain was confirmed by DNA sequencing. The six VNTR loci (Bruce 01, Bruce 14, Bruce 22, Bruce 30, Bruce 71, and Bruce 72) show differentiation in B. abortus human isolates. MLVA = multilocus variable-number tandem-repeat (VNTR) analysis; PCR = polymerase chain reaction.

    Article Snippet: The DNA amplification was performed using 15 μL of solution containing 10 ng of genomic DNA, 1 U of i-Max II DNA polymerase (iNtRON Biotechnology, Gyeonggi-do, Korea), 2.5 mM deoxyribonucleotide triphosphate mixture, 10× polymerase chain reaction (PCR) buffer, 10 pmol of each primer (Bioneer, Daejeon, Korea), 5× betaine (Sigma Aldrich, St. Louis, MO, USA), and distilled sterilized water.

    Techniques: Amplification, Polymerase Chain Reaction, DNA Sequencing

    ( A ) Schematic representation of  S. pombe  chromosome ends and telomeric transcriptome. Telomeric repeats are in black and subtelomeric sequences in white. Oligonucleotides used for RT–PCR and/or northern blotting are indicated by arrows. The sketch is not in scale. ( B ) RNA isolated from wt and Δ rap1  cells was hybridized with the indicated probes. Exposures and signal detection were performed in parallel for TERRA and ARIA as well as for ARRET and αARRET. The black arrowheads indicate two discrete bands of ∼0.7 and 1.1 kb detected by oC and oG oligonucleotides and of so far unknown origin. The asterisks indicate major hybridization bands corresponding to ARRET and αARRET RNA species. U6 snRNA was used as loading control. Molecular weights are on the left in kb. ( C ) Total RNA from wt and Δ rap1  strains was reverse transcribed (RT) using oC (left panels) or o1 (right panels) oligonucleotides and cDNA was PCR amplified using o2+o3 or o2+oC oligonucleotides. The most right samples correspond to control reactions where template was omitted. Molecular weights are on the right in bp.

    Journal: Nucleic Acids Research

    Article Title: The telomeric transcriptome of Schizosaccharomyces pombe

    doi: 10.1093/nar/gkr1153

    Figure Lengend Snippet: ( A ) Schematic representation of S. pombe chromosome ends and telomeric transcriptome. Telomeric repeats are in black and subtelomeric sequences in white. Oligonucleotides used for RT–PCR and/or northern blotting are indicated by arrows. The sketch is not in scale. ( B ) RNA isolated from wt and Δ rap1 cells was hybridized with the indicated probes. Exposures and signal detection were performed in parallel for TERRA and ARIA as well as for ARRET and αARRET. The black arrowheads indicate two discrete bands of ∼0.7 and 1.1 kb detected by oC and oG oligonucleotides and of so far unknown origin. The asterisks indicate major hybridization bands corresponding to ARRET and αARRET RNA species. U6 snRNA was used as loading control. Molecular weights are on the left in kb. ( C ) Total RNA from wt and Δ rap1 strains was reverse transcribed (RT) using oC (left panels) or o1 (right panels) oligonucleotides and cDNA was PCR amplified using o2+o3 or o2+oC oligonucleotides. The most right samples correspond to control reactions where template was omitted. Molecular weights are on the right in bp.

    Article Snippet: Strains and media Original Schizosaccharomyces pombe strains used in this study were kind gifts from Julie Promisel Cooper, Karl Ekwall and Marc Bühler or were purchased from Bioneer Corporation ( ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Northern Blot, Isolation, Hybridization, Polymerase Chain Reaction, Amplification

    DNA marker paradox derived from MTPTs. ( a) Amplicons of a Ca-specific marker (Ca_s_1) designed from the matK region and its counterpart MTPTs in C. wilfordii (Cw) and C. auriculatum (Ca). Pt, plastid genome; mt, MTPT segment in the mitochondrial genome. Primer regions and target SNPs used for authentication of Cw and Ca are marked with red arrows and red stars, respectively. (b) Electrophoresis of PCR products from three authentication markers for Cynanchum species after different numbers of PCR cycles. Ca_s_1 is a Ca dominant marker, and Cw_i_1 and Cw_i_2 are co-dominant markers. Two accessions each of Cw and Ca were used. (c) Results from electrophoresis of PCR products obtained from Cw and Ca samples using the Ca-specific marker Ca_s_1 with different amounts of template DNA and different numbers of PCR cycles. MTPT-derived PCR products are denoted by white and yellow arrowheads in Cw and Ca, respectively.

    Journal: Scientific Reports

    Article Title: Mitochondrial plastid DNA can cause DNA barcoding paradox in plants

    doi: 10.1038/s41598-020-63233-y

    Figure Lengend Snippet: DNA marker paradox derived from MTPTs. ( a) Amplicons of a Ca-specific marker (Ca_s_1) designed from the matK region and its counterpart MTPTs in C. wilfordii (Cw) and C. auriculatum (Ca). Pt, plastid genome; mt, MTPT segment in the mitochondrial genome. Primer regions and target SNPs used for authentication of Cw and Ca are marked with red arrows and red stars, respectively. (b) Electrophoresis of PCR products from three authentication markers for Cynanchum species after different numbers of PCR cycles. Ca_s_1 is a Ca dominant marker, and Cw_i_1 and Cw_i_2 are co-dominant markers. Two accessions each of Cw and Ca were used. (c) Results from electrophoresis of PCR products obtained from Cw and Ca samples using the Ca-specific marker Ca_s_1 with different amounts of template DNA and different numbers of PCR cycles. MTPT-derived PCR products are denoted by white and yellow arrowheads in Cw and Ca, respectively.

    Article Snippet: The mixture consisted of a total volume of 25 μL prepared using an IncloneTM Taq DNA Polymerase Kit (Inclone, South Korea) with 5–80 ng of template DNA, 1× PCR reaction buffer, 0.2 mM of each dNTP, 0.2 pmol of each primer (Bioneer, South Korea) and 0.4 units of Taq DNA polymerase.

    Techniques: Marker, Derivative Assay, Electrophoresis, Polymerase Chain Reaction

    Agarose gel electrophoresis of the amplified HIV gag gene (148 bp) prepared by a general gDNA extraction and on-chip process. The integrated chip could successfully extract the HIV proviral DNA from infected cell populations as low as 10 2 /μl in the blood sample. Lanes: M, molecular weight standard; C, PCR product by chemical method; IT, PCR product by IT chip with respect to sample population; N, negative control.

    Journal: Scientific Reports

    Article Title: On-chip Extraction of Intracellular Molecules in White Blood Cells from Whole Blood

    doi: 10.1038/srep15167

    Figure Lengend Snippet: Agarose gel electrophoresis of the amplified HIV gag gene (148 bp) prepared by a general gDNA extraction and on-chip process. The integrated chip could successfully extract the HIV proviral DNA from infected cell populations as low as 10 2 /μl in the blood sample. Lanes: M, molecular weight standard; C, PCR product by chemical method; IT, PCR product by IT chip with respect to sample population; N, negative control.

    Article Snippet: A gDNA solution of 4 μL was mixed with 1 μL of DNA staining buffer (Loading Star, DyneBio, Korea), together with a 1-kb DNA ladder marker (D-1040, Bioneer, Korea).

    Techniques: Agarose Gel Electrophoresis, Amplification, Chromatin Immunoprecipitation, Infection, Molecular Weight, Polymerase Chain Reaction, Negative Control