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Avantor polymerase chain reaction pcr buffer
Polymerase Chain Reaction Pcr Buffer, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Polymerase Chain Reaction:

Article Title: Impact of an invasive nitrogen-fixing tree on arbuscular mycorrhizal fungi and the development of native species
Article Snippet: .. All reactions were carried out in a final volume of 25 µL containing 1× polymerase chain reaction (PCR) buffer, 2.5 U Taq DNA polymerase (VWR), 0.25 mM dNTP, 0.5 µM AM1, 0.003 g bovine serum albumin, 96 % electrophoresis degree (SIGMA) and 1 µL of extracted genomic DNA. ..

Electrophoresis:

Article Title: Impact of an invasive nitrogen-fixing tree on arbuscular mycorrhizal fungi and the development of native species
Article Snippet: .. All reactions were carried out in a final volume of 25 µL containing 1× polymerase chain reaction (PCR) buffer, 2.5 U Taq DNA polymerase (VWR), 0.25 mM dNTP, 0.5 µM AM1, 0.003 g bovine serum albumin, 96 % electrophoresis degree (SIGMA) and 1 µL of extracted genomic DNA. ..

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    Avantor anti hbs
    Effects of substitutions in Enh-II on the envelope protein expression. Relative ratio of PreS2 and PreS1-mRNA as determined by quantitative real-time PCR ( A ), expression of HBV envelope proteins by immunoblot assay with mouse monoclonal <t>anti-HBs</t> primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( B ); α-Tubulin served as the loading control] ( B ) and <t>HBsAg</t> level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( C ), following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding Enh-II-mutated HBV [HBV/D-mt(Enh-II)] in Huh7 cells. Paired t-test p values; *p
    Anti Hbs, supplied by Avantor, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hbs/product/Avantor
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hbs - by Bioz Stars, 2020-07
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    91
    Avantor gfp intensity
    Chemical screening of the Sox9 inducer from a small molecule library. a Vector generation strategy containing the Sox9 promoter region and a <t>GFP</t> tag. b After the ASCs were transfected with the Sox9 promoter-GFP vector, 1 μM small molecules were added for 24 h. GFP expression was measured using a fluorescence microplate reader. c Selected candidate small molecules were added to ASCs transfected with the Sox9 promoter-GFP vector. GFP expression in the ASCs transfected with the Sox9 promoter-GFP vector was observed by fluorescence microscopy. A pEGFP-N1 vector was used as a positive control to evaluate the <t>transfection</t> efficiency. Chondrogenic differentiation medium (DM) was also used as a positive control to evaluate Sox9 expression. d ASCs were treated with 1 μM of each of the three selected candidate drugs every 3 days for 16 days. The expression of mature chondrocyte markers (Sox9, Aggrecan, and Type II collagen) and hypertrophic chondrocyte markers (RUNX2 and Type X collagen) was measured using PCR of the Drug 7-, Drug 51-, and Drug 138-treated ASCs. D DMSO-treated ASCs, * p
    Gfp Intensity, supplied by Avantor, used in various techniques. Bioz Stars score: 91/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp intensity/product/Avantor
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp intensity - by Bioz Stars, 2020-07
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    80
    Avantor potential rho independent terminators
    Models of ϕX174 transcription. (A) Previous model of øX174 transcription adapted from ( Fane et al., 2005 ). Pink bars represent idealized mRNA quantities with initiation occurring at each known promoter and a step-wise decrease in transcriptional current occurring at each <t>Rho-independent</t> terminator. Gene colors represent broad protein function with red for scaffolding proteins, blue for capsid proteins, yellow for viral propagation proteins, and green for host interacting proteins. Promoters are shown in pink and <t>terminators</t> in red. (B) Previous model of relative transcription of øX174 genes generated from qPCR data ( Brown et al., 2010 ; Zhao et al., 2012 ). (C) RNA-seq measurements of øX174 infection. Read mapping density across the øX174 genome shown by pink bar. Trimmed reads from biological replicates (n=3) mapped to reference øX174 genome (Genbank no. NC_001422.1 ) using Geneious Prime software. (D) RNA-seq measurements of relative transcription of øX174 genes. Values are the average of three biological replicates with error bars showing one standard deviation. Transcripts per million calculated from CDS feature annotations using Geneious Prime.
    Potential Rho Independent Terminators, supplied by Avantor, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/potential rho independent terminators/product/Avantor
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    91
    Avantor serum hbsag
    Effects of substitutions in Enh-II on the envelope protein expression. Relative ratio of PreS2 and PreS1-mRNA as determined by quantitative real-time PCR ( A ), expression of HBV envelope proteins by immunoblot assay with mouse monoclonal <t>anti-HBs</t> primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( B ); α-Tubulin served as the loading control] ( B ) and <t>HBsAg</t> level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( C ), following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding Enh-II-mutated HBV [HBV/D-mt(Enh-II)] in Huh7 cells. Paired t-test p values; *p
    Serum Hbsag, supplied by Avantor, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    Effects of substitutions in Enh-II on the envelope protein expression. Relative ratio of PreS2 and PreS1-mRNA as determined by quantitative real-time PCR ( A ), expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( B ); α-Tubulin served as the loading control] ( B ) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( C ), following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding Enh-II-mutated HBV [HBV/D-mt(Enh-II)] in Huh7 cells. Paired t-test p values; *p

    Journal: Scientific Reports

    Article Title: Synergistic impact of mutations in Hepatitis B Virus genome contribute to its occult phenotype in chronic Hepatitis C Virus carriers

    doi: 10.1038/s41598-017-09965-w

    Figure Lengend Snippet: Effects of substitutions in Enh-II on the envelope protein expression. Relative ratio of PreS2 and PreS1-mRNA as determined by quantitative real-time PCR ( A ), expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( B ); α-Tubulin served as the loading control] ( B ) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( C ), following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding Enh-II-mutated HBV [HBV/D-mt(Enh-II)] in Huh7 cells. Paired t-test p values; *p

    Article Snippet: The tests for serum HBsAg, anti-HBc and anti-HBs were accomplished using BeneSphera HBsAg Microwell ELISA kit (Avantor Performance Materials), DS-EIA-ANTI-HBsAg ELISA kit (DSI S.r.l.) and DS-EIA-ANTI-HBc ELISA kit (DSI S.r.l.) respectively, according to the manufacturers’ protocols.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transfection

    Effects of substitutions in ORF-S on HBsAg antigenicity and its detection. ( A ) Antigenicity plot of “a” determinant (aa.124–147) of HBsAg showing decreased antigenicity due to substitutions T125M and P127T as obtained by Kolaskar and Tongaonkar Antigenicity Prediction method. The relative ratio of PreS2/S and PreS1 mRNA determined by quantitative real-time PCR ( B ), the expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( A ); α-Tubulin served as the loading control] ( C ) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( D ), following transfection of wt-HBV (HBV/D-wt) and mutant construct [HBV/D-mt(HBsAg)], having T125M and P127T substitutions in Huh7 cells. Paired t-test p values; ***p

    Journal: Scientific Reports

    Article Title: Synergistic impact of mutations in Hepatitis B Virus genome contribute to its occult phenotype in chronic Hepatitis C Virus carriers

    doi: 10.1038/s41598-017-09965-w

    Figure Lengend Snippet: Effects of substitutions in ORF-S on HBsAg antigenicity and its detection. ( A ) Antigenicity plot of “a” determinant (aa.124–147) of HBsAg showing decreased antigenicity due to substitutions T125M and P127T as obtained by Kolaskar and Tongaonkar Antigenicity Prediction method. The relative ratio of PreS2/S and PreS1 mRNA determined by quantitative real-time PCR ( B ), the expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( A ); α-Tubulin served as the loading control] ( C ) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( D ), following transfection of wt-HBV (HBV/D-wt) and mutant construct [HBV/D-mt(HBsAg)], having T125M and P127T substitutions in Huh7 cells. Paired t-test p values; ***p

    Article Snippet: The tests for serum HBsAg, anti-HBc and anti-HBs were accomplished using BeneSphera HBsAg Microwell ELISA kit (Avantor Performance Materials), DS-EIA-ANTI-HBsAg ELISA kit (DSI S.r.l.) and DS-EIA-ANTI-HBc ELISA kit (DSI S.r.l.) respectively, according to the manufacturers’ protocols.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Mutagenesis, Construct

    Chemical screening of the Sox9 inducer from a small molecule library. a Vector generation strategy containing the Sox9 promoter region and a GFP tag. b After the ASCs were transfected with the Sox9 promoter-GFP vector, 1 μM small molecules were added for 24 h. GFP expression was measured using a fluorescence microplate reader. c Selected candidate small molecules were added to ASCs transfected with the Sox9 promoter-GFP vector. GFP expression in the ASCs transfected with the Sox9 promoter-GFP vector was observed by fluorescence microscopy. A pEGFP-N1 vector was used as a positive control to evaluate the transfection efficiency. Chondrogenic differentiation medium (DM) was also used as a positive control to evaluate Sox9 expression. d ASCs were treated with 1 μM of each of the three selected candidate drugs every 3 days for 16 days. The expression of mature chondrocyte markers (Sox9, Aggrecan, and Type II collagen) and hypertrophic chondrocyte markers (RUNX2 and Type X collagen) was measured using PCR of the Drug 7-, Drug 51-, and Drug 138-treated ASCs. D DMSO-treated ASCs, * p

    Journal: Experimental & Molecular Medicine

    Article Title: Differentiation of adipose-derived stem cells into functional chondrocytes by a small molecule that induces Sox9

    doi: 10.1038/s12276-020-0424-y

    Figure Lengend Snippet: Chemical screening of the Sox9 inducer from a small molecule library. a Vector generation strategy containing the Sox9 promoter region and a GFP tag. b After the ASCs were transfected with the Sox9 promoter-GFP vector, 1 μM small molecules were added for 24 h. GFP expression was measured using a fluorescence microplate reader. c Selected candidate small molecules were added to ASCs transfected with the Sox9 promoter-GFP vector. GFP expression in the ASCs transfected with the Sox9 promoter-GFP vector was observed by fluorescence microscopy. A pEGFP-N1 vector was used as a positive control to evaluate the transfection efficiency. Chondrogenic differentiation medium (DM) was also used as a positive control to evaluate Sox9 expression. d ASCs were treated with 1 μM of each of the three selected candidate drugs every 3 days for 16 days. The expression of mature chondrocyte markers (Sox9, Aggrecan, and Type II collagen) and hypertrophic chondrocyte markers (RUNX2 and Type X collagen) was measured using PCR of the Drug 7-, Drug 51-, and Drug 138-treated ASCs. D DMSO-treated ASCs, * p

    Article Snippet: After transfection, the GFP intensity was examined under fluorescence microscopy (Glomax® Explorer multimode microplate reader, Promega, WI, USA).

    Techniques: Plasmid Preparation, Transfection, Expressing, Fluorescence, Microscopy, Positive Control, Polymerase Chain Reaction

    Models of ϕX174 transcription. (A) Previous model of øX174 transcription adapted from ( Fane et al., 2005 ). Pink bars represent idealized mRNA quantities with initiation occurring at each known promoter and a step-wise decrease in transcriptional current occurring at each Rho-independent terminator. Gene colors represent broad protein function with red for scaffolding proteins, blue for capsid proteins, yellow for viral propagation proteins, and green for host interacting proteins. Promoters are shown in pink and terminators in red. (B) Previous model of relative transcription of øX174 genes generated from qPCR data ( Brown et al., 2010 ; Zhao et al., 2012 ). (C) RNA-seq measurements of øX174 infection. Read mapping density across the øX174 genome shown by pink bar. Trimmed reads from biological replicates (n=3) mapped to reference øX174 genome (Genbank no. NC_001422.1 ) using Geneious Prime software. (D) RNA-seq measurements of relative transcription of øX174 genes. Values are the average of three biological replicates with error bars showing one standard deviation. Transcripts per million calculated from CDS feature annotations using Geneious Prime.

    Journal: bioRxiv

    Article Title: A high-resolution map of bacteriophage øX174 transcription

    doi: 10.1101/2020.03.05.979765

    Figure Lengend Snippet: Models of ϕX174 transcription. (A) Previous model of øX174 transcription adapted from ( Fane et al., 2005 ). Pink bars represent idealized mRNA quantities with initiation occurring at each known promoter and a step-wise decrease in transcriptional current occurring at each Rho-independent terminator. Gene colors represent broad protein function with red for scaffolding proteins, blue for capsid proteins, yellow for viral propagation proteins, and green for host interacting proteins. Promoters are shown in pink and terminators in red. (B) Previous model of relative transcription of øX174 genes generated from qPCR data ( Brown et al., 2010 ; Zhao et al., 2012 ). (C) RNA-seq measurements of øX174 infection. Read mapping density across the øX174 genome shown by pink bar. Trimmed reads from biological replicates (n=3) mapped to reference øX174 genome (Genbank no. NC_001422.1 ) using Geneious Prime software. (D) RNA-seq measurements of relative transcription of øX174 genes. Values are the average of three biological replicates with error bars showing one standard deviation. Transcripts per million calculated from CDS feature annotations using Geneious Prime.

    Article Snippet: Potential Rho-independent terminators were identified using the following software packages: FindTerm , iTerm-PseKNC , and RibEx: Riboswitch Explorer ( ).

    Techniques: Scaffolding, Generated, Real-time Polymerase Chain Reaction, RNA Sequencing Assay, Infection, Software, Standard Deviation

    Effects of substitutions in Enh-II on the envelope protein expression. Relative ratio of PreS2 and PreS1-mRNA as determined by quantitative real-time PCR ( A ), expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( B ); α-Tubulin served as the loading control] ( B ) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( C ), following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding Enh-II-mutated HBV [HBV/D-mt(Enh-II)] in Huh7 cells. Paired t-test p values; *p

    Journal: Scientific Reports

    Article Title: Synergistic impact of mutations in Hepatitis B Virus genome contribute to its occult phenotype in chronic Hepatitis C Virus carriers

    doi: 10.1038/s41598-017-09965-w

    Figure Lengend Snippet: Effects of substitutions in Enh-II on the envelope protein expression. Relative ratio of PreS2 and PreS1-mRNA as determined by quantitative real-time PCR ( A ), expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( B ); α-Tubulin served as the loading control] ( B ) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( C ), following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding Enh-II-mutated HBV [HBV/D-mt(Enh-II)] in Huh7 cells. Paired t-test p values; *p

    Article Snippet: The tests for serum HBsAg, anti-HBc and anti-HBs were accomplished using BeneSphera HBsAg Microwell ELISA kit (Avantor Performance Materials), DS-EIA-ANTI-HBsAg ELISA kit (DSI S.r.l.) and DS-EIA-ANTI-HBc ELISA kit (DSI S.r.l.) respectively, according to the manufacturers’ protocols.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transfection

    Effects of substitutions in ORF-S on HBsAg antigenicity and its detection. ( A ) Antigenicity plot of “a” determinant (aa.124–147) of HBsAg showing decreased antigenicity due to substitutions T125M and P127T as obtained by Kolaskar and Tongaonkar Antigenicity Prediction method. The relative ratio of PreS2/S and PreS1 mRNA determined by quantitative real-time PCR ( B ), the expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( A ); α-Tubulin served as the loading control] ( C ) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( D ), following transfection of wt-HBV (HBV/D-wt) and mutant construct [HBV/D-mt(HBsAg)], having T125M and P127T substitutions in Huh7 cells. Paired t-test p values; ***p

    Journal: Scientific Reports

    Article Title: Synergistic impact of mutations in Hepatitis B Virus genome contribute to its occult phenotype in chronic Hepatitis C Virus carriers

    doi: 10.1038/s41598-017-09965-w

    Figure Lengend Snippet: Effects of substitutions in ORF-S on HBsAg antigenicity and its detection. ( A ) Antigenicity plot of “a” determinant (aa.124–147) of HBsAg showing decreased antigenicity due to substitutions T125M and P127T as obtained by Kolaskar and Tongaonkar Antigenicity Prediction method. The relative ratio of PreS2/S and PreS1 mRNA determined by quantitative real-time PCR ( B ), the expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 ( A ); α-Tubulin served as the loading control] ( C ) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA ( D ), following transfection of wt-HBV (HBV/D-wt) and mutant construct [HBV/D-mt(HBsAg)], having T125M and P127T substitutions in Huh7 cells. Paired t-test p values; ***p

    Article Snippet: The tests for serum HBsAg, anti-HBc and anti-HBs were accomplished using BeneSphera HBsAg Microwell ELISA kit (Avantor Performance Materials), DS-EIA-ANTI-HBsAg ELISA kit (DSI S.r.l.) and DS-EIA-ANTI-HBc ELISA kit (DSI S.r.l.) respectively, according to the manufacturers’ protocols.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Mutagenesis, Construct