Structured Review

TaKaRa polymerase chain reaction pcr buffer 10x
Polymerase Chain Reaction Pcr Buffer 10x, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr buffer 10x/product/TaKaRa
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr buffer 10x - by Bioz Stars, 2020-07
90/100 stars

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Polymerase Chain Reaction:

Article Title: DNA methylation analysis of secreted frizzled-related protein 2 gene for the early detection of colorectal cancer in fecal DNA
Article Snippet: .. In addition, 17.87 μl O2HDD, 2.5 μl polymerase chain reaction (PCR) buffer 10X, 2-μl dNTP, 0.25 μl forward and 0.25 μl reverse primer, and 0.125 μl TakaRa Taq HS were used in MSP reaction. .. The MSP procedures for SFRP2 gene were performed as follows: 95°C 10 min, 95°C 45 s 45 cycles, 50°C 30 s 45 cycles (annealing temperature for unmethylated primer pairs), 62°C 30 s 45 cycles (annealing temperature for methylated primer pairs), 72°C for 30 s, and 72°C 5 min for final extension.

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    TaKaRa pcr buffer
    Multiplex nested <t>RT-PCR</t> targeting Pfs25 and Pvs25 . Representative 2% agarose gel electrophoresis showing specific amplifications generated from secondary PCR. Lane M, 50-bp ladder marker; lanes 1–6, P. falciparum genomic DNA, P. vivax genomic DNA, water as negative control, P. falciparum <t>cDNA</t> , P. vivax cDNA and mixed P. falciparum and P. vivax cDNA, respectively. Molecular size in base pairs is shown on the left and right of the gel.
    Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/TaKaRa
    Average 95 stars, based on 1840 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    99
    TaKaRa 10x taq buffer
    Multiplex nested <t>RT-PCR</t> targeting Pfs25 and Pvs25 . Representative 2% agarose gel electrophoresis showing specific amplifications generated from secondary PCR. Lane M, 50-bp ladder marker; lanes 1–6, P. falciparum genomic DNA, P. vivax genomic DNA, water as negative control, P. falciparum <t>cDNA</t> , P. vivax cDNA and mixed P. falciparum and P. vivax cDNA, respectively. Molecular size in base pairs is shown on the left and right of the gel.
    10x Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x taq buffer/product/TaKaRa
    Average 99 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    10x taq buffer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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    Multiplex nested RT-PCR targeting Pfs25 and Pvs25 . Representative 2% agarose gel electrophoresis showing specific amplifications generated from secondary PCR. Lane M, 50-bp ladder marker; lanes 1–6, P. falciparum genomic DNA, P. vivax genomic DNA, water as negative control, P. falciparum cDNA , P. vivax cDNA and mixed P. falciparum and P. vivax cDNA, respectively. Molecular size in base pairs is shown on the left and right of the gel.

    Journal: Malaria Journal

    Article Title: Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR

    doi: 10.1186/1475-2875-11-190

    Figure Lengend Snippet: Multiplex nested RT-PCR targeting Pfs25 and Pvs25 . Representative 2% agarose gel electrophoresis showing specific amplifications generated from secondary PCR. Lane M, 50-bp ladder marker; lanes 1–6, P. falciparum genomic DNA, P. vivax genomic DNA, water as negative control, P. falciparum cDNA , P. vivax cDNA and mixed P. falciparum and P. vivax cDNA, respectively. Molecular size in base pairs is shown on the left and right of the gel.

    Article Snippet: The optimal DNA amplification was carried out in a total volume of 20 μL containing 2 μL of cDNA template, 2.5 mM each deoxynucleotide triphosphate, 2 μL of 10x PCR buffer, 1.6 μL of 25 mM MgCl2 , 0.08 μL of 30 μM of each primer for primary PCR and 0.4 units of rTaq DNA polymerase (Takara, Seta, Japan).

    Techniques: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Generated, Polymerase Chain Reaction, Marker, Negative Control

    Nuclear DNA content obtained by flow cytometry and MET1 / DDM1 transcription analysis. (A) Nuclear DNA content of Chrysanthemum nankingense (2x), C. indicum (4x), C. morifolium (6x), Ajania shiwogiku (8x) and C. crassum (10x); (B) The Ct value and melting temperature of each reference genes; the X axis represents the PCR cycle number. The red line represents the threshold fluorescence at which the Ct was determined. None of the reference genes were uniformly transcribed (Ct) in five ploidy levels and species. Melting curve analyses showed that each primer pair amplified a single PCR product. (C) Model of reference gene(s) used in five ploidy levels and species; (D) Transcript abundance was correlated with ploidy level for both MET1 and DDM1 . PP2A was selected as inter-run calibrators (IRCs) for EF1 α, TUB , and ACTIN , and the coefficient of variation (transcript abundance normalized using EF1 α 4x-6x -TUB 6x-8x -ACTIN 8x-10x vs. using PP2A 4x-8x-10x )

    Journal: Frontiers in Plant Science

    Article Title: Limited DNA methylation variation and the transcription of MET1 and DDM1 in the genus Chrysanthemum (Asteraceae): following the track of polyploidy

    doi: 10.3389/fpls.2015.00668

    Figure Lengend Snippet: Nuclear DNA content obtained by flow cytometry and MET1 / DDM1 transcription analysis. (A) Nuclear DNA content of Chrysanthemum nankingense (2x), C. indicum (4x), C. morifolium (6x), Ajania shiwogiku (8x) and C. crassum (10x); (B) The Ct value and melting temperature of each reference genes; the X axis represents the PCR cycle number. The red line represents the threshold fluorescence at which the Ct was determined. None of the reference genes were uniformly transcribed (Ct) in five ploidy levels and species. Melting curve analyses showed that each primer pair amplified a single PCR product. (C) Model of reference gene(s) used in five ploidy levels and species; (D) Transcript abundance was correlated with ploidy level for both MET1 and DDM1 . PP2A was selected as inter-run calibrators (IRCs) for EF1 α, TUB , and ACTIN , and the coefficient of variation (transcript abundance normalized using EF1 α 4x-6x -TUB 6x-8x -ACTIN 8x-10x vs. using PP2A 4x-8x-10x )

    Article Snippet: A 5 μL aliquot of the product was pre-amplified in the presence of 0.2 μM Eco RI and 0.2 μM Hpa II-Msp I non-selective primers (sequences given in Supplementary Table ) in a 25 μL reaction containing 2.5 μL 10x PCR buffer, 1.5 mM MgCl2 , 0.2 mM dNTP, and 2 U Taq polymerase (Takara, Japan).

    Techniques: Flow Cytometry, Cytometry, Polymerase Chain Reaction, Fluorescence, Amplification