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Roche polymerase chain reaction pcr assay
Polymerase Chain Reaction Pcr Assay, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr assay/product/Roche
Average 91 stars, based on 28 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr assay - by Bioz Stars, 2020-04
91/100 stars

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Diagnostic Assay:

Article Title: Scaling sexual behavior or "sexual risk propensity" among men at risk for HIV in Kisumu, Kenya
Article Snippet: .. An STI was defined by a laboratory-based diagnosis of gonorrhea, chlamydial infection or trichomoniasis, using the following diagnostic criteria: Neisseria gonorrhoeae (Ng) and Chlamydia trachomatis (CT): by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal Canada) and Trichomonas vaginalis (TV): by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). ..

Real-time Polymerase Chain Reaction:

Article Title: Which patients respond best to hepatitis B vaccination after a hepatitis B virus-related liver transplantation?
Article Snippet: .. HBV-DNA levels were measured using a transcription-mediated amplification assay (TMA) (SRL, Tokyo, Japan), a polymerase chain reaction (PCR) assay (Amplicor HBV Monitor assay; Roche Diagnostics, Tokyo, Japan), or a real-time PCR assay (COBAS TaqMan HBV Test; Roche Diagnostics). .. HBV recombinant proteins for cellular immune response analysis Hepatitis B virus recombinant protein HBsAg was purchased from Advanced ImmunoChemical, Inc. (Long Beach, CA).

Article Title: Serum hepatitis B virus DNA before liver transplantation correlates with HBV reinfection rate even under successful low-dose hepatitis B immunoglobulin prophylaxis
Article Snippet: .. Serum HBV-DNA assay HBV-DNA level was measured using a transcription-mediated amplification assay (TMA) (SRL, Tokyo, Japan), polymerase chain reaction (PCR) assay (Amplicor HBV Monitor assay; Roche Diagnostics, Tokyo, Japan) or real-time PCR assay (COBAS TaqMan HBV Test; Roche Diagnostics). ..

Article Title: Expression Pattern of Serum Cytokines in Hepatitis B Virus Infected Patients with Persistently Normal Alanine Aminotransferase Levels
Article Snippet: .. Serum HBV DNA levels were quantified using a commercially available polymerase chain reaction (PCR) assay (LightCycler®480 Real-Time PCR System, Roche, Basel, Switzerland) with a lower limit of quantification of 200 IU/ml. ..

Flow Cytometry:

Article Title: The Combination of the Gastrointestinal Integrin (?4?7) and Selectin Ligand Enhances T Cell Migration to the Reproductive Tract during Infection with C. trachomatis
Article Snippet: We developed a method to identify leukocyte subsets in endocervical brush samples and peripheral blood mononuclear cells (PBMC) using flow cytometry. .. The subjects were categorized as STI positive by polymerase chain reaction (PCR) assay of endocervical swabs for Neiserria gonorrhoeae and Chlamydia trachomatis (Hoffmann-La Roche Ltd Roche, Basel, Switzerland).

Article Title: Bone mass and mineral metabolism in HIV+ postmenopausal women
Article Snippet: CD4 counts were measured by flow cytometry (FACS Calibur, Becton Dickinson, San Jose, CA, USA). .. HIV-1 RNA was quantified by a polymerase chain reaction (PCR) assay utilizing the AMPLICOR HIV-1 MONITOR UltraSensitive Test (Version 1.5) with a linear range of 50–100,000 copies/ml (Roche Diagnostics, Indianapolis, IN, USA).

Amplification:

Article Title: Which patients respond best to hepatitis B vaccination after a hepatitis B virus-related liver transplantation?
Article Snippet: .. HBV-DNA levels were measured using a transcription-mediated amplification assay (TMA) (SRL, Tokyo, Japan), a polymerase chain reaction (PCR) assay (Amplicor HBV Monitor assay; Roche Diagnostics, Tokyo, Japan), or a real-time PCR assay (COBAS TaqMan HBV Test; Roche Diagnostics). .. HBV recombinant proteins for cellular immune response analysis Hepatitis B virus recombinant protein HBsAg was purchased from Advanced ImmunoChemical, Inc. (Long Beach, CA).

Article Title: Serum hepatitis B virus DNA before liver transplantation correlates with HBV reinfection rate even under successful low-dose hepatitis B immunoglobulin prophylaxis
Article Snippet: .. Serum HBV-DNA assay HBV-DNA level was measured using a transcription-mediated amplification assay (TMA) (SRL, Tokyo, Japan), polymerase chain reaction (PCR) assay (Amplicor HBV Monitor assay; Roche Diagnostics, Tokyo, Japan) or real-time PCR assay (COBAS TaqMan HBV Test; Roche Diagnostics). ..

Article Title: The Effect of Medical Male Circumcision on Urogenital Mycoplasma genitalium among Men in Kisumu, Kenya
Article Snippet: For detection of Mycoplasma genitalium , an aliquot of first void urine was placed in the appropriate GEN-PROBE Specimen Transport Media for the APTIMA transcription mediated amplification (TMA)-based assay (GEN-PROBE, Inc, San Diego, CA). .. First void urine specimens were also tested for Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal, Canada), for Trichomonas vaginalis (TV) by TMA, and for TV by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States).

Enzyme Immunoassay:

Article Title: Expression Pattern of Serum Cytokines in Hepatitis B Virus Infected Patients with Persistently Normal Alanine Aminotransferase Levels
Article Snippet: Antibodies to hepatitis C virus, hepatitis D virus and human immunodeficiency virus were detected by routine, commercially available enzyme immunoassays (KHB, Shanghai, China). .. Serum HBV DNA levels were quantified using a commercially available polymerase chain reaction (PCR) assay (LightCycler®480 Real-Time PCR System, Roche, Basel, Switzerland) with a lower limit of quantification of 200 IU/ml.

Isolation:

Article Title: Serum Fibrosis Marker Levels Decrease After Successful Antiviral Treatment in Chronic Hepatitis C Patients with Advanced Fibrosis
Article Snippet: Subjects who remained viremic at week 20 were eligible for randomization to maintenance peginterferon versus no treatment for 3.5 years; subjects with undetectable HCV RNA at week 20, as determined by polymerase chain reaction (PCR) assay (Roche Molecular Systems, COBAS Amplicor v 2.0, sensitivity of 100 IU/ml) continued in the “responder arm” of the study and completed a 48 week course of combination antiviral treatment. .. Serum isolated from whole blood samples was frozen immediately at −80° C and stored at a central repository (Seracare, Washington, DC).

AST Assay:

Article Title: Precore and Core Promoter Mutations of the Hepatitis B Virus Gene in Chronic Genotype C -Infected Children
Article Snippet: Immune clearance phase was defined by abnormal AST/AST level, HBeAg positive and falling serum HBV DNA concentrations ( ). .. HBV DNA levels were quantified by a polymerase chain reaction (PCR) assay with a lower limit of detection of 2×102 copies/mL and a linearity range of 2 × 102 -2 × 105 copies/mL (Cobas Amplicor HBV Monitor, Roche Diagnostic Systems, Pleasanton, CA, USA).

Cytometry:

Article Title: The Combination of the Gastrointestinal Integrin (?4?7) and Selectin Ligand Enhances T Cell Migration to the Reproductive Tract during Infection with C. trachomatis
Article Snippet: We developed a method to identify leukocyte subsets in endocervical brush samples and peripheral blood mononuclear cells (PBMC) using flow cytometry. .. The subjects were categorized as STI positive by polymerase chain reaction (PCR) assay of endocervical swabs for Neiserria gonorrhoeae and Chlamydia trachomatis (Hoffmann-La Roche Ltd Roche, Basel, Switzerland).

Article Title: Bone mass and mineral metabolism in HIV+ postmenopausal women
Article Snippet: CD4 counts were measured by flow cytometry (FACS Calibur, Becton Dickinson, San Jose, CA, USA). .. HIV-1 RNA was quantified by a polymerase chain reaction (PCR) assay utilizing the AMPLICOR HIV-1 MONITOR UltraSensitive Test (Version 1.5) with a linear range of 50–100,000 copies/ml (Roche Diagnostics, Indianapolis, IN, USA).

Infection:

Article Title: Prediction of Week 4 Virological Response in Hepatitis C for Making Decision on Triple Therapy: The Optim Study
Article Snippet: Exclusion criteria were: previous treatment with P+R; any coexisting chronic liver disease (including HBV infection); HCV genotypes other than 1 & 4 (i.e. types 2, 3, 5 and 6) ( ). .. At baseline, all patients had a quantitative measurement of serum or plasma HCV-RNA performed using the polymerase chain reaction (PCR) assay with the COBAS AmpliPrep/COBAS TaqMan HCV Test (Roche Diagnostics GMBH, Mannheim).

Article Title: Adult Male Circumcision Does Not Reduce Risk of Incident Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis: Results from a Randomized Controlled Trial in Kenya
Article Snippet: Paragraph title: Sexually Transmitted Infection Testing ... At baseline and at 6, 12, 18, and 24 month follow-up visits, participants were asked to provide urine specimens, which were tested for Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal, Canada), and for Trichomonas vaginalis (TV) by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States).

Article Title: The Effect of Medical Male Circumcision on Urogenital Mycoplasma genitalium among Men in Kisumu, Kenya
Article Snippet: Paragraph title: Sexually Transmitted Infection and HIV Testing ... First void urine specimens were also tested for Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal, Canada), for Trichomonas vaginalis (TV) by TMA, and for TV by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States).

Article Title: Scaling sexual behavior or "sexual risk propensity" among men at risk for HIV in Kisumu, Kenya
Article Snippet: .. An STI was defined by a laboratory-based diagnosis of gonorrhea, chlamydial infection or trichomoniasis, using the following diagnostic criteria: Neisseria gonorrhoeae (Ng) and Chlamydia trachomatis (CT): by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal Canada) and Trichomonas vaginalis (TV): by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). ..

Article Title: Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay.
Article Snippet: Screening for Chlamydia trachomatis infection in men has traditionally been limited to men who present with urethral symptoms, thereby limiting the detection of asymptomatic chlamydia infection in men. .. In order to effectively screen both symptomatic and asymptomatic men, we evaluated a newly developed polymerase chain reaction (PCR) assay, Amplicor C. trachomatis, from Roche Molecular Systems for the detection of C. trachomatis in urine specimens in comparison with urethral culture.

Treponema Pallidum Haemagglutination Assay:

Article Title: The Effect of Medical Male Circumcision on Urogenital Mycoplasma genitalium among Men in Kisumu, Kenya
Article Snippet: First void urine specimens were also tested for Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal, Canada), for Trichomonas vaginalis (TV) by TMA, and for TV by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). .. Serum specimens were tested for syphilis antibody (rapid plasma reagin with TPHA confirmation) and herpes simplex virus type 2 (HSV-2) antibody (Kalon HSV-2 IgG ELISA, Kalon Biological Limited, Aldershot, United Kingdom).

Enzyme-linked Immunosorbent Assay:

Article Title: Which patients respond best to hepatitis B vaccination after a hepatitis B virus-related liver transplantation?
Article Snippet: Routine laboratory tests and serum HBV-DNA assay Hepatitis B surface antigen, anti-HBs antibody, hepatitis Be antigen (HBeAg), and anti-HBe antibody (HBeAb) levels were measured routinely using a commercially available chemiluminescent enzyme immunoassay system (Lumipulse System; Fujirebio, Tokyo, Japan). .. HBV-DNA levels were measured using a transcription-mediated amplification assay (TMA) (SRL, Tokyo, Japan), a polymerase chain reaction (PCR) assay (Amplicor HBV Monitor assay; Roche Diagnostics, Tokyo, Japan), or a real-time PCR assay (COBAS TaqMan HBV Test; Roche Diagnostics).

Article Title: Bone mass and mineral metabolism in HIV+ postmenopausal women
Article Snippet: Serum N-telopeptide (NTX) was measured using a competitive-inhibition, enzyme-linked immunosorbent assay (ELISA, Osteo-mark, Ostex, Seattle, WA, USA) [ ]. .. HIV-1 RNA was quantified by a polymerase chain reaction (PCR) assay utilizing the AMPLICOR HIV-1 MONITOR UltraSensitive Test (Version 1.5) with a linear range of 50–100,000 copies/ml (Roche Diagnostics, Indianapolis, IN, USA).

Article Title: The Effect of Medical Male Circumcision on Urogenital Mycoplasma genitalium among Men in Kisumu, Kenya
Article Snippet: First void urine specimens were also tested for Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal, Canada), for Trichomonas vaginalis (TV) by TMA, and for TV by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). .. Serum specimens were tested for syphilis antibody (rapid plasma reagin with TPHA confirmation) and herpes simplex virus type 2 (HSV-2) antibody (Kalon HSV-2 IgG ELISA, Kalon Biological Limited, Aldershot, United Kingdom).

Article Title: Scaling sexual behavior or "sexual risk propensity" among men at risk for HIV in Kisumu, Kenya
Article Snippet: An STI was defined by a laboratory-based diagnosis of gonorrhea, chlamydial infection or trichomoniasis, using the following diagnostic criteria: Neisseria gonorrhoeae (Ng) and Chlamydia trachomatis (CT): by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal Canada) and Trichomonas vaginalis (TV): by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). .. Men with discordant rapid tests results underwent further testing by double ELISA (Detect HIV 1/2, Adaltis Inc, Montreal, Canada, and Recombigen HIV 1/2, Trinity Biotech, Wicklow, Ireland).

Polymerase Chain Reaction:

Article Title: Which patients respond best to hepatitis B vaccination after a hepatitis B virus-related liver transplantation?
Article Snippet: .. HBV-DNA levels were measured using a transcription-mediated amplification assay (TMA) (SRL, Tokyo, Japan), a polymerase chain reaction (PCR) assay (Amplicor HBV Monitor assay; Roche Diagnostics, Tokyo, Japan), or a real-time PCR assay (COBAS TaqMan HBV Test; Roche Diagnostics). .. HBV recombinant proteins for cellular immune response analysis Hepatitis B virus recombinant protein HBsAg was purchased from Advanced ImmunoChemical, Inc. (Long Beach, CA).

Article Title: Prediction of Week 4 Virological Response in Hepatitis C for Making Decision on Triple Therapy: The Optim Study
Article Snippet: .. At baseline, all patients had a quantitative measurement of serum or plasma HCV-RNA performed using the polymerase chain reaction (PCR) assay with the COBAS AmpliPrep/COBAS TaqMan HCV Test (Roche Diagnostics GMBH, Mannheim). ..

Article Title: Serum hepatitis B virus DNA before liver transplantation correlates with HBV reinfection rate even under successful low-dose hepatitis B immunoglobulin prophylaxis
Article Snippet: .. Serum HBV-DNA assay HBV-DNA level was measured using a transcription-mediated amplification assay (TMA) (SRL, Tokyo, Japan), polymerase chain reaction (PCR) assay (Amplicor HBV Monitor assay; Roche Diagnostics, Tokyo, Japan) or real-time PCR assay (COBAS TaqMan HBV Test; Roche Diagnostics). ..

Article Title: The Combination of the Gastrointestinal Integrin (?4?7) and Selectin Ligand Enhances T Cell Migration to the Reproductive Tract during Infection with C. trachomatis
Article Snippet: .. The subjects were categorized as STI positive by polymerase chain reaction (PCR) assay of endocervical swabs for Neiserria gonorrhoeae and Chlamydia trachomatis (Hoffmann-La Roche Ltd Roche, Basel, Switzerland). ..

Article Title: Bone mass and mineral metabolism in HIV+ postmenopausal women
Article Snippet: .. HIV-1 RNA was quantified by a polymerase chain reaction (PCR) assay utilizing the AMPLICOR HIV-1 MONITOR UltraSensitive Test (Version 1.5) with a linear range of 50–100,000 copies/ml (Roche Diagnostics, Indianapolis, IN, USA). ..

Article Title: Adult Male Circumcision Does Not Reduce Risk of Incident Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis: Results from a Randomized Controlled Trial in Kenya
Article Snippet: .. At baseline and at 6, 12, 18, and 24 month follow-up visits, participants were asked to provide urine specimens, which were tested for Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal, Canada), and for Trichomonas vaginalis (TV) by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). .. Men with urethral discharge also had a urethral swab taken for PCR testing for NG and CT, and culture for NG and TV.

Article Title: Serum Fibrosis Marker Levels Decrease After Successful Antiviral Treatment in Chronic Hepatitis C Patients with Advanced Fibrosis
Article Snippet: .. Subjects who remained viremic at week 20 were eligible for randomization to maintenance peginterferon versus no treatment for 3.5 years; subjects with undetectable HCV RNA at week 20, as determined by polymerase chain reaction (PCR) assay (Roche Molecular Systems, COBAS Amplicor v 2.0, sensitivity of 100 IU/ml) continued in the “responder arm” of the study and completed a 48 week course of combination antiviral treatment. ..

Article Title: The Effect of Medical Male Circumcision on Urogenital Mycoplasma genitalium among Men in Kisumu, Kenya
Article Snippet: .. First void urine specimens were also tested for Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal, Canada), for Trichomonas vaginalis (TV) by TMA, and for TV by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). .. Men with urethral discharge had a urethral swab taken for detection of NG and CT by PCR, and for detection of NG and TV by culture.

Article Title: Scaling sexual behavior or "sexual risk propensity" among men at risk for HIV in Kisumu, Kenya
Article Snippet: .. An STI was defined by a laboratory-based diagnosis of gonorrhea, chlamydial infection or trichomoniasis, using the following diagnostic criteria: Neisseria gonorrhoeae (Ng) and Chlamydia trachomatis (CT): by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal Canada) and Trichomonas vaginalis (TV): by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). ..

Article Title: Expression Pattern of Serum Cytokines in Hepatitis B Virus Infected Patients with Persistently Normal Alanine Aminotransferase Levels
Article Snippet: .. Serum HBV DNA levels were quantified using a commercially available polymerase chain reaction (PCR) assay (LightCycler®480 Real-Time PCR System, Roche, Basel, Switzerland) with a lower limit of quantification of 200 IU/ml. ..

Article Title: Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay.
Article Snippet: .. In order to effectively screen both symptomatic and asymptomatic men, we evaluated a newly developed polymerase chain reaction (PCR) assay, Amplicor C. trachomatis, from Roche Molecular Systems for the detection of C. trachomatis in urine specimens in comparison with urethral culture. ..

Nested PCR:

Article Title: Precore and Core Promoter Mutations of the Hepatitis B Virus Gene in Chronic Genotype C -Infected Children
Article Snippet: HBV DNA levels were quantified by a polymerase chain reaction (PCR) assay with a lower limit of detection of 2×102 copies/mL and a linearity range of 2 × 102 -2 × 105 copies/mL (Cobas Amplicor HBV Monitor, Roche Diagnostic Systems, Pleasanton, CA, USA). .. The extracted HBV DNA was immediately used for genotyping and nested PCR.

Intra Assay:

Article Title: Bone mass and mineral metabolism in HIV+ postmenopausal women
Article Snippet: The intra-assay and inter-assay variabilities are 4.6% and 6.9%, respectively. .. HIV-1 RNA was quantified by a polymerase chain reaction (PCR) assay utilizing the AMPLICOR HIV-1 MONITOR UltraSensitive Test (Version 1.5) with a linear range of 50–100,000 copies/ml (Roche Diagnostics, Indianapolis, IN, USA).

Modification:

Article Title: Prediction of Week 4 Virological Response in Hepatitis C for Making Decision on Triple Therapy: The Optim Study
Article Snippet: Patients received peginterferon α-2a (Pegasys; Roche 180μ/week) combined with ribavirin 1,000 mg/day if body weight was ≤75kg, or 1,200mg if body weight was > 75 kg. Peginterferon and ribavirin dose modification were according to standard criteria and procedures [ ]. .. At baseline, all patients had a quantitative measurement of serum or plasma HCV-RNA performed using the polymerase chain reaction (PCR) assay with the COBAS AmpliPrep/COBAS TaqMan HCV Test (Roche Diagnostics GMBH, Mannheim).

FACS:

Article Title: Bone mass and mineral metabolism in HIV+ postmenopausal women
Article Snippet: CD4 counts were measured by flow cytometry (FACS Calibur, Becton Dickinson, San Jose, CA, USA). .. HIV-1 RNA was quantified by a polymerase chain reaction (PCR) assay utilizing the AMPLICOR HIV-1 MONITOR UltraSensitive Test (Version 1.5) with a linear range of 50–100,000 copies/ml (Roche Diagnostics, Indianapolis, IN, USA).

Recombinant:

Article Title: Scaling sexual behavior or "sexual risk propensity" among men at risk for HIV in Kisumu, Kenya
Article Snippet: An STI was defined by a laboratory-based diagnosis of gonorrhea, chlamydial infection or trichomoniasis, using the following diagnostic criteria: Neisseria gonorrhoeae (Ng) and Chlamydia trachomatis (CT): by polymerase chain reaction (PCR) assay (AMPLICOR® CT/NG Test, Roche Diagnostics, Montreal Canada) and Trichomonas vaginalis (TV): by culture (InPouch™ TV test, Biomed Diagnostics, Oregon, United States). .. Men were tested for HIV-1 using two parallel rapid tests: Determine HIV 1/2 (Abbott Diagnostic Division, Hoofddorp, Netherlands) and the recombinant antigen test Unigold Recombigen HIV Test (Trinity Biotech, Wicklow, Ireland).

Inter Assay:

Article Title: Bone mass and mineral metabolism in HIV+ postmenopausal women
Article Snippet: The intra-assay and inter-assay variabilities are 4.6% and 6.9%, respectively. .. HIV-1 RNA was quantified by a polymerase chain reaction (PCR) assay utilizing the AMPLICOR HIV-1 MONITOR UltraSensitive Test (Version 1.5) with a linear range of 50–100,000 copies/ml (Roche Diagnostics, Indianapolis, IN, USA).

Electrochemiluminescence:

Article Title: Expression Pattern of Serum Cytokines in Hepatitis B Virus Infected Patients with Persistently Normal Alanine Aminotransferase Levels
Article Snippet: HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc, and alpha-fetoprotein levels were detected by Electrochemiluminescence (Architect, Abbott Laboratories, Abbott Park, IL, USA). .. Serum HBV DNA levels were quantified using a commercially available polymerase chain reaction (PCR) assay (LightCycler®480 Real-Time PCR System, Roche, Basel, Switzerland) with a lower limit of quantification of 200 IU/ml.

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  • 93
    Roche telomerase activity assays cancer cells
    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding <t>telomerase</t> activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.
    Telomerase Activity Assays Cancer Cells, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/telomerase activity assays cancer cells/product/Roche
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    telomerase activity assays cancer cells - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    Roche plasma hbv dna
    Comparison of <t>HBV</t> replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV <t>DNA</t> from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p
    Plasma Hbv Dna, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma hbv dna/product/Roche
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    plasma hbv dna - by Bioz Stars, 2020-04
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    94
    Roche p malariae strains greece
    Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. <t>malariae</t> (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control
    P Malariae Strains Greece, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 3 article reviews
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    gfp  (Roche)
    99
    Roche gfp
    Colocalization and effects of direct NPFFR2 signalling on <t>NPY</t> neurons. a Representative image of <t>GFP</t> expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p
    Gfp, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 70 article reviews
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    Image Search Results


    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Journal: Oncotarget

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    doi: 10.18632/oncotarget.25993

    Figure Lengend Snippet: STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Article Snippet: Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA.

    Techniques: Binding Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Journal: Oncotarget

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    doi: 10.18632/oncotarget.25993

    Figure Lengend Snippet: Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Article Snippet: Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA.

    Techniques: Activity Assay

    Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Journal: Viruses

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    doi: 10.3390/v11010078

    Figure Lengend Snippet: Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Article Snippet: The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

    Techniques: Mutagenesis, Transfection, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Journal: Viruses

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    doi: 10.3390/v11010078

    Figure Lengend Snippet: Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Article Snippet: The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

    Techniques: Mutagenesis, MANN-WHITNEY

    Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Titration, Infection, Incubation, Multiplex Assay, Concentration Assay

    Antibody competition titration assays using homologous MSP1 19 proteins. Dilutions (1:400) of P. falciparum Lot 6 defined human serum or of sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) were incubated with the indicated concentrations of the homologous MSP1 19 competitor protein for 1 h at room temperature. Multiplex bead assays were performed as described in “ Methods ”, and the multiplex responses in MFI-bg units are plotted versus the competitor concentration

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Antibody competition titration assays using homologous MSP1 19 proteins. Dilutions (1:400) of P. falciparum Lot 6 defined human serum or of sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) were incubated with the indicated concentrations of the homologous MSP1 19 competitor protein for 1 h at room temperature. Multiplex bead assays were performed as described in “ Methods ”, and the multiplex responses in MFI-bg units are plotted versus the competitor concentration

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Titration, Infection, Incubation, Multiplex Assay, Concentration Assay

    Alignment of predicted Plasmodium spp. MSP1 19 protein sequences using COBALT [ 61 ]. Residues in the P. malariae sequence that differ from the Cameroon sequence of Birkenmeyer et al. [ 38 ] are shaded. Predicted protein sequences resulting from the oligonucleotides used in PCR amplification are underlined. The positions of residues conserved among all the presented MSP1 19 protein sequences are indicated in the consensus with divergent residues indicated by a dot. GenBank accession numbers are MH577181, P. ovale Nigeria I strain; MH577182, P. malariae China I strain; MH577183, P. malariae Greece I strain; MH577184, P. malariae Uganda I strain; and MH577185, P. malariae Guyana strain

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Alignment of predicted Plasmodium spp. MSP1 19 protein sequences using COBALT [ 61 ]. Residues in the P. malariae sequence that differ from the Cameroon sequence of Birkenmeyer et al. [ 38 ] are shaded. Predicted protein sequences resulting from the oligonucleotides used in PCR amplification are underlined. The positions of residues conserved among all the presented MSP1 19 protein sequences are indicated in the consensus with divergent residues indicated by a dot. GenBank accession numbers are MH577181, P. ovale Nigeria I strain; MH577182, P. malariae China I strain; MH577183, P. malariae Greece I strain; MH577184, P. malariae Uganda I strain; and MH577185, P. malariae Guyana strain

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification

    Colocalization and effects of direct NPFFR2 signalling on NPY neurons. a Representative image of GFP expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p

    Journal: Nature Communications

    Article Title: Diet-induced adaptive thermogenesis requires neuropeptide FF receptor-2 signalling

    doi: 10.1038/s41467-018-06462-0

    Figure Lengend Snippet: Colocalization and effects of direct NPFFR2 signalling on NPY neurons. a Representative image of GFP expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p

    Article Snippet: RT-qPCR using primers for Npy , GFP and Npffr2 was carried out in samples prior (input) and after the immunoprecipitation in at least triplicates from 1:5 dilution cDNA from each sample using the LightCycler® (LightCycler® 480 Real-Time PCR system, Roche Applied Science, Germany), SYBR Green I (Molecular Probes) and Platinum Taq DNA Polymerase (Invitrogen).

    Techniques: Expressing, Immunoprecipitation, Isolation, Mouse Assay