polymerase chain reaction pcr amplified fragments  (Roche)

 
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    Structured Review

    Roche polymerase chain reaction pcr amplified fragments
    Polymerase Chain Reaction Pcr Amplified Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr amplified fragments/product/Roche
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr amplified fragments - by Bioz Stars, 2020-07
    85/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Tandem Repeat-Containing MITEs in the Clam Donax trunculus
    Article Snippet: .. Polymerase chain reaction (PCR)-amplified fragments of interest were labeled with digoxigenin by random priming using the DIG DNA Labeling and Detection Kit (Roche) and used as a hybridization probe. ..

    Article Title: Genome Structure of the Legume, Lotus japonicus
    Article Snippet: .. For subtraction, polymerase chain reaction (PCR)-amplified fragments of LjTR1 were biotinylated using Biotin-High Prime (Roche, Basel, Switzerland) and used as a driver in subtractive hybridization with the WGS library. .. The WGS library was single-stranded prior to hybridization by combined action of gene II and exonuclease III.

    DNA Labeling:

    Article Title: Tandem Repeat-Containing MITEs in the Clam Donax trunculus
    Article Snippet: .. Polymerase chain reaction (PCR)-amplified fragments of interest were labeled with digoxigenin by random priming using the DIG DNA Labeling and Detection Kit (Roche) and used as a hybridization probe. ..

    Labeling:

    Article Title: Tandem Repeat-Containing MITEs in the Clam Donax trunculus
    Article Snippet: .. Polymerase chain reaction (PCR)-amplified fragments of interest were labeled with digoxigenin by random priming using the DIG DNA Labeling and Detection Kit (Roche) and used as a hybridization probe. ..

    Hybridization:

    Article Title: Tandem Repeat-Containing MITEs in the Clam Donax trunculus
    Article Snippet: .. Polymerase chain reaction (PCR)-amplified fragments of interest were labeled with digoxigenin by random priming using the DIG DNA Labeling and Detection Kit (Roche) and used as a hybridization probe. ..

    Article Title: Genome Structure of the Legume, Lotus japonicus
    Article Snippet: .. For subtraction, polymerase chain reaction (PCR)-amplified fragments of LjTR1 were biotinylated using Biotin-High Prime (Roche, Basel, Switzerland) and used as a driver in subtractive hybridization with the WGS library. .. The WGS library was single-stranded prior to hybridization by combined action of gene II and exonuclease III.

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    Roche pcr amplified hpt ii gene fragment
    Molecular analysis of ATM transformed S. miltiorrhiza . (A) <t>PCR</t> analysis of transformerd Salvia plant. Genomic DNA was amplified using <t>hpt</t> II gene specific primers. Approx. 650 bp amplification was observed in transformed plant. Lanes: (M) Molecular weight marker, (1) Positive control (pTAG8 plasmid), (2) Wild type Salvia ; and (3) Active mutagenic Salvia plant (SH41). (B) Confirmation of activation-tagged S. miltiorrhiza Bunge by Southern blot analysis. Genomic DNA was extracted from leaves and hpt II gene used as a probe. Hybridization was performed at 52°C. V- pTAG-8; SP- SH41 and WP- WT digested by Pst I; SH- SH41 and WH- WT digested by Hind III; SE- SH41 and WE- WT digested by EcoR I. Arrows indicate hybridization signals.
    Pcr Amplified Hpt Ii Gene Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    91
    Roche nrps fragments
    Doughnut chart summarising the proposition of <t>NRPS</t> and <t>PKS-II</t> positive isolates among the distinct actinobacterial isolates affiliated to different genus. The two levels of ripples around the doughnut represent the percentage of Actinobacteria that were positive for NRPS (inner ripple) and PKS-II (outer ripple). The number in the centre of doughnut indicates total actinobacterial isolates tested.
    Nrps Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    93
    Roche dig labelled pcr fragments
    Quantification of PDE4A, PDE4B and PDE4D in CD8 lymphocytes from both healthy and asthmatic subjects. An <t>ELISA</t> technique was used on fragments amplified by <t>RT–PCR</t> (sample sizes vary: PDE4A=10 μl sample, PDE4B=10 μl sample, PDE4D=1 μl sample). Readings were taken as absorbance at 402 nm after 30 min incubation with the substrate and data are expressed in arbitrary units (subtype absorbance/β actin absorbance). In each case, values are expressed as mean±s.e.mean ( n =6). None of these results were found to be statistically significant ( P > 0.05).
    Dig Labelled Pcr Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dig labelled pcr fragments/product/Roche
    Average 93 stars, based on 2 article reviews
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    94
    Roche pcr dig probe synthesis kit
    Molecular analysis of the Plcd3 mNab mutation. A. Schematic representation of the genomic context of the Plcd3 mNab mutation. The Plcd3 mNab mutation is caused by the insertion of 5.4 kbp of IAP sequences in intron 2 of Plcd3 . Hybridisation with a probe (P) derived from intron 1 using primers 572 and 573 ( Table S1 ) reveals a restriction fragment length polymorphism in BamHI digested genomic DNA of wild-type (+/+), oltSH (+/−) and oltNH (−/−) mice, respectively. The wild-type allele shows a 4.1 kbp fragment and the mutant allele a fragment of 4.5 kbp. The increased length of the mutant fragment is caused by the BamHI site (B) within the inserted IAP. The Plcd3 locus is shown in reverse orientation with respect to the chromosomal DNA, with Plcd3 exon 1 to the left. The intracisternal A particle (IAP) has no env sequences and is inserted in intron 2 in reverse orientation with respect to the transcription of the Plcd3 gene. B. Location of the primers used to analyse the Plcd3 mNab mutant and the wild-type Plcd3 locus (f, forward; r, reverse). On the left, electrophoresis of amplified genomic DNA fragments that are indicative of the wild-type Plcd3 and the Plcd3 mNab allele using the primers indicated. On the right, the <t>PCR</t> products from genomic DNA around Plcd3 intron 2 obtained from wild-type and oltNH mice using primers f1046 and r1049 ( Table S1 ) are shown. The 5.4 kbp long fragment in the oltNH mutant contains the inserted IAP sequence. C. Northern blot analysis of mutant dorsal skin. A <t>DIG-labelled</t> probe derived from the 5′ region of Plcd1 hybridises to transcripts only in phenotypically wild-type animals. A DIG-labelled RNA probe derived from the 3′ region of Plcd3 hybridises to two transcripts of 3 kb and 2.6 kb, respectively (marked by arrowheads). oltSH mice express both transcripts, while oltNH mice show only the 2.6 kb transcript. Note that the wild-type mouse in lane 2 shows the mutant Plcd3 transcript and expresses Plcd1 . Hybridisation with a Gapdh -specific probe as loading control is also shown. D. Western blot analysis of mutant dorsal skin using antibody IF12–15 directed against the catalytic region of PLCD3 protein and 4H 5–9 directed against the PH domain of PLCD3. The phenotypes of the mice (Wt, oltNH , oltSH ), from which the lysates were obtained, are given on top of each lane. The 2.6 kb mutant Plcd3 transcript is translated to a truncated protein of 75 kDa, which is detected by the antibody IF12–15, but not the PH domain-specific antibody 4H 5–9 (lanes 2 and 3). This antibody also bound to an unknown protein of 110 kDa in all samples. Arrowheads indicate the wild-type protein. An immunoblot using antibody against actin is given below as a control. Note that the phenotypically wild-type in lane 2 is the same as the one in lane 2 of the Northern blot in C.
    Pcr Dig Probe Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr dig probe synthesis kit/product/Roche
    Average 94 stars, based on 255 article reviews
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    Image Search Results


    Molecular analysis of ATM transformed S. miltiorrhiza . (A) PCR analysis of transformerd Salvia plant. Genomic DNA was amplified using hpt II gene specific primers. Approx. 650 bp amplification was observed in transformed plant. Lanes: (M) Molecular weight marker, (1) Positive control (pTAG8 plasmid), (2) Wild type Salvia ; and (3) Active mutagenic Salvia plant (SH41). (B) Confirmation of activation-tagged S. miltiorrhiza Bunge by Southern blot analysis. Genomic DNA was extracted from leaves and hpt II gene used as a probe. Hybridization was performed at 52°C. V- pTAG-8; SP- SH41 and WP- WT digested by Pst I; SH- SH41 and WH- WT digested by Hind III; SE- SH41 and WE- WT digested by EcoR I. Arrows indicate hybridization signals.

    Journal: Botanical Studies

    Article Title: Activation tagging in Salvia miltiorrhiza can cause increased leaf size and accumulation of tanshinone I and IIA in its roots

    doi: 10.1186/1999-3110-54-37

    Figure Lengend Snippet: Molecular analysis of ATM transformed S. miltiorrhiza . (A) PCR analysis of transformerd Salvia plant. Genomic DNA was amplified using hpt II gene specific primers. Approx. 650 bp amplification was observed in transformed plant. Lanes: (M) Molecular weight marker, (1) Positive control (pTAG8 plasmid), (2) Wild type Salvia ; and (3) Active mutagenic Salvia plant (SH41). (B) Confirmation of activation-tagged S. miltiorrhiza Bunge by Southern blot analysis. Genomic DNA was extracted from leaves and hpt II gene used as a probe. Hybridization was performed at 52°C. V- pTAG-8; SP- SH41 and WP- WT digested by Pst I; SH- SH41 and WH- WT digested by Hind III; SE- SH41 and WE- WT digested by EcoR I. Arrows indicate hybridization signals.

    Article Snippet: A PCR-amplified hpt II gene fragment was labelled with digoxigenin-9-dUTP using Dig High Prime Labeling and Detection Starter Kit I (Roche, USA) and used as a probe.

    Techniques: Transformation Assay, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker, Positive Control, Plasmid Preparation, Activation Assay, Southern Blot, Hybridization

    Doughnut chart summarising the proposition of NRPS and PKS-II positive isolates among the distinct actinobacterial isolates affiliated to different genus. The two levels of ripples around the doughnut represent the percentage of Actinobacteria that were positive for NRPS (inner ripple) and PKS-II (outer ripple). The number in the centre of doughnut indicates total actinobacterial isolates tested.

    Journal: Scientific Reports

    Article Title: Intertidal marine sediment harbours Actinobacteria with promising bioactive and biosynthetic potential

    doi: 10.1038/s41598-017-09672-6

    Figure Lengend Snippet: Doughnut chart summarising the proposition of NRPS and PKS-II positive isolates among the distinct actinobacterial isolates affiliated to different genus. The two levels of ripples around the doughnut represent the percentage of Actinobacteria that were positive for NRPS (inner ripple) and PKS-II (outer ripple). The number in the centre of doughnut indicates total actinobacterial isolates tested.

    Article Snippet: Cloning and sequencing of the amplified KS and NRPS fragments The amplified PKS-II and NRPS fragments (PCR products) were purified using the High Pure PCR product purification kit (Roche Molecular, Basel, Switzerland), following the manufacturer’s protocols.

    Techniques:

    Correlation of antibacterial activity and occurrence of NRPS and PKS-II systems visualized by cytoscape network image.

    Journal: Scientific Reports

    Article Title: Intertidal marine sediment harbours Actinobacteria with promising bioactive and biosynthetic potential

    doi: 10.1038/s41598-017-09672-6

    Figure Lengend Snippet: Correlation of antibacterial activity and occurrence of NRPS and PKS-II systems visualized by cytoscape network image.

    Article Snippet: Cloning and sequencing of the amplified KS and NRPS fragments The amplified PKS-II and NRPS fragments (PCR products) were purified using the High Pure PCR product purification kit (Roche Molecular, Basel, Switzerland), following the manufacturer’s protocols.

    Techniques: Activity Assay

    Quantification of PDE4A, PDE4B and PDE4D in CD8 lymphocytes from both healthy and asthmatic subjects. An ELISA technique was used on fragments amplified by RT–PCR (sample sizes vary: PDE4A=10 μl sample, PDE4B=10 μl sample, PDE4D=1 μl sample). Readings were taken as absorbance at 402 nm after 30 min incubation with the substrate and data are expressed in arbitrary units (subtype absorbance/β actin absorbance). In each case, values are expressed as mean±s.e.mean ( n =6). None of these results were found to be statistically significant ( P > 0.05).

    Journal: British Journal of Pharmacology

    Article Title: Identification and quantification of phosphodiesterase 4 subtypes in CD4 and CD8 lymphocytes from healthy and asthmatic subjects

    doi: 10.1038/sj.bjp.0704120

    Figure Lengend Snippet: Quantification of PDE4A, PDE4B and PDE4D in CD8 lymphocytes from both healthy and asthmatic subjects. An ELISA technique was used on fragments amplified by RT–PCR (sample sizes vary: PDE4A=10 μl sample, PDE4B=10 μl sample, PDE4D=1 μl sample). Readings were taken as absorbance at 402 nm after 30 min incubation with the substrate and data are expressed in arbitrary units (subtype absorbance/β actin absorbance). In each case, values are expressed as mean±s.e.mean ( n =6). None of these results were found to be statistically significant ( P > 0.05).

    Article Snippet: For this purpose, amplified dig-labelled PCR fragments were analysed using the PCR ELISA Dig Detection kit from Roche Diagnostics (Meylan, France).

    Techniques: Enzyme-linked Immunosorbent Assay, Amplification, Reverse Transcription Polymerase Chain Reaction, Incubation

    Quantification of PDE4A, PDE4B and PDE4D in CD4 lymphocytes from both healthy and asthmatic subjects. An ELISA technique was used on fragments amplified by RT–PCR (sample sizes vary: PDE4A=5 μl sample, PDE4B=1 μl sample, PDE4D=0.5 μl sample). Readings were taken as absorbance at 402 nm after 30 min incubation with the substrate and data are expressed in arbitrary units (subtype absorbance/β actin absorbance). In each case, values are expressed as mean±s.e.mean ( n =10). None of these results were found to be statistically significant ( P > 0.05).

    Journal: British Journal of Pharmacology

    Article Title: Identification and quantification of phosphodiesterase 4 subtypes in CD4 and CD8 lymphocytes from healthy and asthmatic subjects

    doi: 10.1038/sj.bjp.0704120

    Figure Lengend Snippet: Quantification of PDE4A, PDE4B and PDE4D in CD4 lymphocytes from both healthy and asthmatic subjects. An ELISA technique was used on fragments amplified by RT–PCR (sample sizes vary: PDE4A=5 μl sample, PDE4B=1 μl sample, PDE4D=0.5 μl sample). Readings were taken as absorbance at 402 nm after 30 min incubation with the substrate and data are expressed in arbitrary units (subtype absorbance/β actin absorbance). In each case, values are expressed as mean±s.e.mean ( n =10). None of these results were found to be statistically significant ( P > 0.05).

    Article Snippet: For this purpose, amplified dig-labelled PCR fragments were analysed using the PCR ELISA Dig Detection kit from Roche Diagnostics (Meylan, France).

    Techniques: Enzyme-linked Immunosorbent Assay, Amplification, Reverse Transcription Polymerase Chain Reaction, Incubation

    Molecular analysis of the Plcd3 mNab mutation. A. Schematic representation of the genomic context of the Plcd3 mNab mutation. The Plcd3 mNab mutation is caused by the insertion of 5.4 kbp of IAP sequences in intron 2 of Plcd3 . Hybridisation with a probe (P) derived from intron 1 using primers 572 and 573 ( Table S1 ) reveals a restriction fragment length polymorphism in BamHI digested genomic DNA of wild-type (+/+), oltSH (+/−) and oltNH (−/−) mice, respectively. The wild-type allele shows a 4.1 kbp fragment and the mutant allele a fragment of 4.5 kbp. The increased length of the mutant fragment is caused by the BamHI site (B) within the inserted IAP. The Plcd3 locus is shown in reverse orientation with respect to the chromosomal DNA, with Plcd3 exon 1 to the left. The intracisternal A particle (IAP) has no env sequences and is inserted in intron 2 in reverse orientation with respect to the transcription of the Plcd3 gene. B. Location of the primers used to analyse the Plcd3 mNab mutant and the wild-type Plcd3 locus (f, forward; r, reverse). On the left, electrophoresis of amplified genomic DNA fragments that are indicative of the wild-type Plcd3 and the Plcd3 mNab allele using the primers indicated. On the right, the PCR products from genomic DNA around Plcd3 intron 2 obtained from wild-type and oltNH mice using primers f1046 and r1049 ( Table S1 ) are shown. The 5.4 kbp long fragment in the oltNH mutant contains the inserted IAP sequence. C. Northern blot analysis of mutant dorsal skin. A DIG-labelled probe derived from the 5′ region of Plcd1 hybridises to transcripts only in phenotypically wild-type animals. A DIG-labelled RNA probe derived from the 3′ region of Plcd3 hybridises to two transcripts of 3 kb and 2.6 kb, respectively (marked by arrowheads). oltSH mice express both transcripts, while oltNH mice show only the 2.6 kb transcript. Note that the wild-type mouse in lane 2 shows the mutant Plcd3 transcript and expresses Plcd1 . Hybridisation with a Gapdh -specific probe as loading control is also shown. D. Western blot analysis of mutant dorsal skin using antibody IF12–15 directed against the catalytic region of PLCD3 protein and 4H 5–9 directed against the PH domain of PLCD3. The phenotypes of the mice (Wt, oltNH , oltSH ), from which the lysates were obtained, are given on top of each lane. The 2.6 kb mutant Plcd3 transcript is translated to a truncated protein of 75 kDa, which is detected by the antibody IF12–15, but not the PH domain-specific antibody 4H 5–9 (lanes 2 and 3). This antibody also bound to an unknown protein of 110 kDa in all samples. Arrowheads indicate the wild-type protein. An immunoblot using antibody against actin is given below as a control. Note that the phenotypically wild-type in lane 2 is the same as the one in lane 2 of the Northern blot in C.

    Journal: PLoS ONE

    Article Title: Alopecia in a Viable Phospholipase C Delta 1 and Phospholipase C Delta 3 Double Mutant

    doi: 10.1371/journal.pone.0039203

    Figure Lengend Snippet: Molecular analysis of the Plcd3 mNab mutation. A. Schematic representation of the genomic context of the Plcd3 mNab mutation. The Plcd3 mNab mutation is caused by the insertion of 5.4 kbp of IAP sequences in intron 2 of Plcd3 . Hybridisation with a probe (P) derived from intron 1 using primers 572 and 573 ( Table S1 ) reveals a restriction fragment length polymorphism in BamHI digested genomic DNA of wild-type (+/+), oltSH (+/−) and oltNH (−/−) mice, respectively. The wild-type allele shows a 4.1 kbp fragment and the mutant allele a fragment of 4.5 kbp. The increased length of the mutant fragment is caused by the BamHI site (B) within the inserted IAP. The Plcd3 locus is shown in reverse orientation with respect to the chromosomal DNA, with Plcd3 exon 1 to the left. The intracisternal A particle (IAP) has no env sequences and is inserted in intron 2 in reverse orientation with respect to the transcription of the Plcd3 gene. B. Location of the primers used to analyse the Plcd3 mNab mutant and the wild-type Plcd3 locus (f, forward; r, reverse). On the left, electrophoresis of amplified genomic DNA fragments that are indicative of the wild-type Plcd3 and the Plcd3 mNab allele using the primers indicated. On the right, the PCR products from genomic DNA around Plcd3 intron 2 obtained from wild-type and oltNH mice using primers f1046 and r1049 ( Table S1 ) are shown. The 5.4 kbp long fragment in the oltNH mutant contains the inserted IAP sequence. C. Northern blot analysis of mutant dorsal skin. A DIG-labelled probe derived from the 5′ region of Plcd1 hybridises to transcripts only in phenotypically wild-type animals. A DIG-labelled RNA probe derived from the 3′ region of Plcd3 hybridises to two transcripts of 3 kb and 2.6 kb, respectively (marked by arrowheads). oltSH mice express both transcripts, while oltNH mice show only the 2.6 kb transcript. Note that the wild-type mouse in lane 2 shows the mutant Plcd3 transcript and expresses Plcd1 . Hybridisation with a Gapdh -specific probe as loading control is also shown. D. Western blot analysis of mutant dorsal skin using antibody IF12–15 directed against the catalytic region of PLCD3 protein and 4H 5–9 directed against the PH domain of PLCD3. The phenotypes of the mice (Wt, oltNH , oltSH ), from which the lysates were obtained, are given on top of each lane. The 2.6 kb mutant Plcd3 transcript is translated to a truncated protein of 75 kDa, which is detected by the antibody IF12–15, but not the PH domain-specific antibody 4H 5–9 (lanes 2 and 3). This antibody also bound to an unknown protein of 110 kDa in all samples. Arrowheads indicate the wild-type protein. An immunoblot using antibody against actin is given below as a control. Note that the phenotypically wild-type in lane 2 is the same as the one in lane 2 of the Northern blot in C.

    Article Snippet: DIG labelled probes for DNA blot hybridisations were synthesised by amplifying genomic fragments using the PCR DIG probe synthesis kit (Roche).

    Techniques: Mutagenesis, Hybridization, Derivative Assay, Mouse Assay, Electrophoresis, Amplification, Polymerase Chain Reaction, Sequencing, Northern Blot, Western Blot