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Real Biotech Corporation polymerase chain reaction genomic dna
Polymerase Chain Reaction Genomic Dna, supplied by Real Biotech Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction genomic dna/product/Real Biotech Corporation
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction genomic dna - by Bioz Stars, 2020-05
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DNA Extraction:

Article Title: MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars
Article Snippet: .. DNA extraction and polymerase chain reaction Genomic DNA was extracted from young leaves of 6-week-old plants selected using 1 mg L−1 PPT with the RBC HiYield™ Genomic DNA Mini Kit (Real Biotech Corporation, Taiwan), according to manufacturer’s instructions. .. Genomic DNA was then amplified using polymerase chain reaction (PCR) with specific primers and PCR conditions mentioned in Table .

Polymerase Chain Reaction:

Article Title: MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars
Article Snippet: .. DNA extraction and polymerase chain reaction Genomic DNA was extracted from young leaves of 6-week-old plants selected using 1 mg L−1 PPT with the RBC HiYield™ Genomic DNA Mini Kit (Real Biotech Corporation, Taiwan), according to manufacturer’s instructions. .. Genomic DNA was then amplified using polymerase chain reaction (PCR) with specific primers and PCR conditions mentioned in Table .

Article Title: Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India
Article Snippet: .. Identification of bacterial species by PCR Genomic DNA was extracted using the HiYield™ Genomic DNA Mini Kit (Real Biotech Corporation, Banqiao city, Taiwan) following the manufacturer’s instructions. .. The Staphylococcus and Streptococcus genus-specific tuf genes as well as the E . coli species-specific alr gene were amplified by monoplex PCR [ , ].

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    Real Biotech Corporation pcr genomic dna
    Polymerase chain reaction for the identification of bacteria. Genomic <t>DNA</t> was isolated from the obtained isolates as well as reference strains, and subjected to mono- or multi-plex <t>PCR</t> as described in the Materials and Methods and Table 1 . The experiments were repeated at least three times and representative gel pictures are shown. Note that each panel is composed from two separate gels since all the samples could not be accommodated in a single gel. (A) PCR for genus-specific tuf genes of streptococci and staphylococci. Lane designation: M, 100 bp ladder; 1–5, Streptococcus spp. isolates; 6, Reference strain Streptococcus AD1; 7, No template control for streptococcus; 8, Negative control ( S . aureus , E . coli ); 9, Reagent control; 10, Reference strain S . aureus 96; 11, No template control for staphylococcus; 12–18: Staphylococcus spp. isolates. PCR for S . aureus nuc (lanes 1–11) and E . coli alr (lanes 12–21) genes. Lane designation: M, 100 bp ladder; 1–8, S . aureus test isolates; 9, Reference strain SAU-3; 10, Negative control ( E . coli ); 11, No template control; 12, Negative control ( S . aureus ); 13, Reference strain EC11 ( E . coli ); 14–16, Test isolates of E . coli ; 17, No template control; 18–20, Test isolates; 21, Negative control (streptococcus). (B) PCR for the identification of CoNS species. Lane designation: M, 100 bp ladder; 1, S . haemolyticus (MTCC 3383) control; 2, S . sciuri (MTCC 6154) control; 3, S . saprophyticus (MTCC 6155) control; 4, S . arlettae (JQ764624) control; 5, S . chromogenes (MTCC 3545) control; 6, S . sciuri (MTCC 6154) control; 7, S . xylosus (FJ90627.1) control; 8, S . simulans (AF495498.1) control; 9, S . epidermidis (MTCC 3615) control; 10, S . haemolyticus (MTCC 3383) control; 11, S . sciuri (MTCC 6154) control; 12, S . saprophyticus (MTCC 6155) control; 13, S . arlettae (JQ764624) control; 14, S . chromogenes (MTCC 3545) control; 15, S . sciuri (MTCC 6154) control; 16, S . simulans (AF495498.1) control; 17, S . xylosus (FJ90627.1) control; 18, S . epidermidis (MTCC 3615) control. This Panel represents two mutually exclusive pictures depicting the results of the standardization of one tube each of the two-tube multiplex PCR. In the left panel, primers for S . arlettae , S . chromogenes , S . sciuri , S . epidermidis and S . saprophyticus were used, and S . haemolyticus , S . xylosus and S . simulans DNA served as negative controls. In the right panel, primers for S . equorum , S . haemolyticus , S . xylosus , S . simulans and S . fluerettii were used, and S . sciuri , S . sapryphyticus , S . arlettae , S . chromogenes and S . epidermidis DNA served as negative controls. Numbers in parentheses indicate the GenBank Accession numbers or the MTCC culture designations. (C) PCR for the identification of Streptococcus species. Lane designation: M, 100 bp ladder; 1–20, Test streptococcal isolates streptococci (no amplification); 21, Negative control ( S . aureus ); 22, Negative control ( E . coli ); 23 24, No template control; 25, Tube 2 positive control ( Streptococcus reference strain AD3); 26, Tube 1 positive controls ( Streptococcus reference strains AD1 and AD6).
    Pcr Genomic Dna, supplied by Real Biotech Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr genomic dna/product/Real Biotech Corporation
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr genomic dna - by Bioz Stars, 2020-05
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    91
    Real Biotech Corporation methylation sensitive high resolution melting ms hrm analysis total genomic dna
    <t>DNA</t> methylation at DMRs of imprinting genes as predicted by <t>HRM</t> software. HRM difference plots for ( A ) SNRPN ( B ) PEG1 0 ( C ) MEST . Each representing three difference plots: first one for methylation standards in different colors (M100% - M0%, which stands for DNA methylation standards with 100 to 0% methylation) normalized to the 0%-methylated standard DNA, second and third difference plots for few selected samples from placental villous groups JEG-3 cells and maternal leukocyte groups represented in different colors and methylated standard curve of M100 to 0% represented as black curves. Note: “Trim” stands for trimester.
    Methylation Sensitive High Resolution Melting Ms Hrm Analysis Total Genomic Dna, supplied by Real Biotech Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methylation sensitive high resolution melting ms hrm analysis total genomic dna/product/Real Biotech Corporation
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    methylation sensitive high resolution melting ms hrm analysis total genomic dna - by Bioz Stars, 2020-05
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    86
    Real Biotech Corporation mtdna cox1 regions genomic dna
    PCR-RFLP analysis of <t>mtDNA</t> <t>cox1</t> for molecular identification of anisakid larvae. Each PCR product was digested with Hinf I and analyzed on agarose gel. Lanes 1-3, A. pegreffii (each from S. schlegeli , C. myriaster , and H. otakii , respectively); Lanes 4-6, A. simplex s. str. (each from G. macrocephalus , O. masou masou , and O. keta , respectively); Lane M, 100 bp ladder.
    Mtdna Cox1 Regions Genomic Dna, supplied by Real Biotech Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtdna cox1 regions genomic dna/product/Real Biotech Corporation
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    mtdna cox1 regions genomic dna - by Bioz Stars, 2020-05
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    92
    Real Biotech Corporation hiyield genomic dna mini kit
    PCR-RFLP analysis of <t>mtDNA</t> <t>cox1</t> for molecular identification of anisakid larvae. Each PCR product was digested with Hinf I and analyzed on agarose gel. Lanes 1-3, A. pegreffii (each from S. schlegeli , C. myriaster , and H. otakii , respectively); Lanes 4-6, A. simplex s. str. (each from G. macrocephalus , O. masou masou , and O. keta , respectively); Lane M, 100 bp ladder.
    Hiyield Genomic Dna Mini Kit, supplied by Real Biotech Corporation, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiyield genomic dna mini kit/product/Real Biotech Corporation
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    Price from $9.99 to $1999.99
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    Polymerase chain reaction for the identification of bacteria. Genomic DNA was isolated from the obtained isolates as well as reference strains, and subjected to mono- or multi-plex PCR as described in the Materials and Methods and Table 1 . The experiments were repeated at least three times and representative gel pictures are shown. Note that each panel is composed from two separate gels since all the samples could not be accommodated in a single gel. (A) PCR for genus-specific tuf genes of streptococci and staphylococci. Lane designation: M, 100 bp ladder; 1–5, Streptococcus spp. isolates; 6, Reference strain Streptococcus AD1; 7, No template control for streptococcus; 8, Negative control ( S . aureus , E . coli ); 9, Reagent control; 10, Reference strain S . aureus 96; 11, No template control for staphylococcus; 12–18: Staphylococcus spp. isolates. PCR for S . aureus nuc (lanes 1–11) and E . coli alr (lanes 12–21) genes. Lane designation: M, 100 bp ladder; 1–8, S . aureus test isolates; 9, Reference strain SAU-3; 10, Negative control ( E . coli ); 11, No template control; 12, Negative control ( S . aureus ); 13, Reference strain EC11 ( E . coli ); 14–16, Test isolates of E . coli ; 17, No template control; 18–20, Test isolates; 21, Negative control (streptococcus). (B) PCR for the identification of CoNS species. Lane designation: M, 100 bp ladder; 1, S . haemolyticus (MTCC 3383) control; 2, S . sciuri (MTCC 6154) control; 3, S . saprophyticus (MTCC 6155) control; 4, S . arlettae (JQ764624) control; 5, S . chromogenes (MTCC 3545) control; 6, S . sciuri (MTCC 6154) control; 7, S . xylosus (FJ90627.1) control; 8, S . simulans (AF495498.1) control; 9, S . epidermidis (MTCC 3615) control; 10, S . haemolyticus (MTCC 3383) control; 11, S . sciuri (MTCC 6154) control; 12, S . saprophyticus (MTCC 6155) control; 13, S . arlettae (JQ764624) control; 14, S . chromogenes (MTCC 3545) control; 15, S . sciuri (MTCC 6154) control; 16, S . simulans (AF495498.1) control; 17, S . xylosus (FJ90627.1) control; 18, S . epidermidis (MTCC 3615) control. This Panel represents two mutually exclusive pictures depicting the results of the standardization of one tube each of the two-tube multiplex PCR. In the left panel, primers for S . arlettae , S . chromogenes , S . sciuri , S . epidermidis and S . saprophyticus were used, and S . haemolyticus , S . xylosus and S . simulans DNA served as negative controls. In the right panel, primers for S . equorum , S . haemolyticus , S . xylosus , S . simulans and S . fluerettii were used, and S . sciuri , S . sapryphyticus , S . arlettae , S . chromogenes and S . epidermidis DNA served as negative controls. Numbers in parentheses indicate the GenBank Accession numbers or the MTCC culture designations. (C) PCR for the identification of Streptococcus species. Lane designation: M, 100 bp ladder; 1–20, Test streptococcal isolates streptococci (no amplification); 21, Negative control ( S . aureus ); 22, Negative control ( E . coli ); 23 24, No template control; 25, Tube 2 positive control ( Streptococcus reference strain AD3); 26, Tube 1 positive controls ( Streptococcus reference strains AD1 and AD6).

    Journal: PLoS ONE

    Article Title: Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

    doi: 10.1371/journal.pone.0142717

    Figure Lengend Snippet: Polymerase chain reaction for the identification of bacteria. Genomic DNA was isolated from the obtained isolates as well as reference strains, and subjected to mono- or multi-plex PCR as described in the Materials and Methods and Table 1 . The experiments were repeated at least three times and representative gel pictures are shown. Note that each panel is composed from two separate gels since all the samples could not be accommodated in a single gel. (A) PCR for genus-specific tuf genes of streptococci and staphylococci. Lane designation: M, 100 bp ladder; 1–5, Streptococcus spp. isolates; 6, Reference strain Streptococcus AD1; 7, No template control for streptococcus; 8, Negative control ( S . aureus , E . coli ); 9, Reagent control; 10, Reference strain S . aureus 96; 11, No template control for staphylococcus; 12–18: Staphylococcus spp. isolates. PCR for S . aureus nuc (lanes 1–11) and E . coli alr (lanes 12–21) genes. Lane designation: M, 100 bp ladder; 1–8, S . aureus test isolates; 9, Reference strain SAU-3; 10, Negative control ( E . coli ); 11, No template control; 12, Negative control ( S . aureus ); 13, Reference strain EC11 ( E . coli ); 14–16, Test isolates of E . coli ; 17, No template control; 18–20, Test isolates; 21, Negative control (streptococcus). (B) PCR for the identification of CoNS species. Lane designation: M, 100 bp ladder; 1, S . haemolyticus (MTCC 3383) control; 2, S . sciuri (MTCC 6154) control; 3, S . saprophyticus (MTCC 6155) control; 4, S . arlettae (JQ764624) control; 5, S . chromogenes (MTCC 3545) control; 6, S . sciuri (MTCC 6154) control; 7, S . xylosus (FJ90627.1) control; 8, S . simulans (AF495498.1) control; 9, S . epidermidis (MTCC 3615) control; 10, S . haemolyticus (MTCC 3383) control; 11, S . sciuri (MTCC 6154) control; 12, S . saprophyticus (MTCC 6155) control; 13, S . arlettae (JQ764624) control; 14, S . chromogenes (MTCC 3545) control; 15, S . sciuri (MTCC 6154) control; 16, S . simulans (AF495498.1) control; 17, S . xylosus (FJ90627.1) control; 18, S . epidermidis (MTCC 3615) control. This Panel represents two mutually exclusive pictures depicting the results of the standardization of one tube each of the two-tube multiplex PCR. In the left panel, primers for S . arlettae , S . chromogenes , S . sciuri , S . epidermidis and S . saprophyticus were used, and S . haemolyticus , S . xylosus and S . simulans DNA served as negative controls. In the right panel, primers for S . equorum , S . haemolyticus , S . xylosus , S . simulans and S . fluerettii were used, and S . sciuri , S . sapryphyticus , S . arlettae , S . chromogenes and S . epidermidis DNA served as negative controls. Numbers in parentheses indicate the GenBank Accession numbers or the MTCC culture designations. (C) PCR for the identification of Streptococcus species. Lane designation: M, 100 bp ladder; 1–20, Test streptococcal isolates streptococci (no amplification); 21, Negative control ( S . aureus ); 22, Negative control ( E . coli ); 23 24, No template control; 25, Tube 2 positive control ( Streptococcus reference strain AD3); 26, Tube 1 positive controls ( Streptococcus reference strains AD1 and AD6).

    Article Snippet: Identification of bacterial species by PCR Genomic DNA was extracted using the HiYield™ Genomic DNA Mini Kit (Real Biotech Corporation, Banqiao city, Taiwan) following the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Isolation, Negative Control, Multiplex Assay, Amplification, Positive Control

    DNA methylation at DMRs of imprinting genes as predicted by HRM software. HRM difference plots for ( A ) SNRPN ( B ) PEG1 0 ( C ) MEST . Each representing three difference plots: first one for methylation standards in different colors (M100% - M0%, which stands for DNA methylation standards with 100 to 0% methylation) normalized to the 0%-methylated standard DNA, second and third difference plots for few selected samples from placental villous groups JEG-3 cells and maternal leukocyte groups represented in different colors and methylated standard curve of M100 to 0% represented as black curves. Note: “Trim” stands for trimester.

    Journal: Scientific Reports

    Article Title: Epigenetic modifications at DMRs of placental genes are subjected to variations in normal gestation, pathological conditions and folate supplementation

    doi: 10.1038/srep40774

    Figure Lengend Snippet: DNA methylation at DMRs of imprinting genes as predicted by HRM software. HRM difference plots for ( A ) SNRPN ( B ) PEG1 0 ( C ) MEST . Each representing three difference plots: first one for methylation standards in different colors (M100% - M0%, which stands for DNA methylation standards with 100 to 0% methylation) normalized to the 0%-methylated standard DNA, second and third difference plots for few selected samples from placental villous groups JEG-3 cells and maternal leukocyte groups represented in different colors and methylated standard curve of M100 to 0% represented as black curves. Note: “Trim” stands for trimester.

    Article Snippet: Methylation-Sensitive High Resolution Melting (MS-HRM) analysis Total genomic DNA isolated by DNA isolation kit (Real Genomics, Real Biotech Corporation, Taipei, Taiwan), from placental villi samples, maternal blood leukocytes, JEG-3 and HTR/SVneo cell lines, circulating DNA isolated by Miniprep DNA isolation kit (Bioserve, MD, USA), from maternal plasma were used to estimate the DMR/promoter region methylation percentage by MS-HRM (methylation sensitive high resolution melting analysis) as described previously .

    Techniques: DNA Methylation Assay, Software, Methylation

    Regulation of differential mRNA expression via DNA methylation in placental cell lines, isolated cytotrophoblasts and normal first trimester placental villi. ( A ) qRT-PCR analysis of candidate tumor suppressor genes. ( B ) MS-HRM analysis of candidate tumor suppressor genes. ‡‡ p

    Journal: Scientific Reports

    Article Title: Epigenetic modifications at DMRs of placental genes are subjected to variations in normal gestation, pathological conditions and folate supplementation

    doi: 10.1038/srep40774

    Figure Lengend Snippet: Regulation of differential mRNA expression via DNA methylation in placental cell lines, isolated cytotrophoblasts and normal first trimester placental villi. ( A ) qRT-PCR analysis of candidate tumor suppressor genes. ( B ) MS-HRM analysis of candidate tumor suppressor genes. ‡‡ p

    Article Snippet: Methylation-Sensitive High Resolution Melting (MS-HRM) analysis Total genomic DNA isolated by DNA isolation kit (Real Genomics, Real Biotech Corporation, Taipei, Taiwan), from placental villi samples, maternal blood leukocytes, JEG-3 and HTR/SVneo cell lines, circulating DNA isolated by Miniprep DNA isolation kit (Bioserve, MD, USA), from maternal plasma were used to estimate the DMR/promoter region methylation percentage by MS-HRM (methylation sensitive high resolution melting analysis) as described previously .

    Techniques: Expressing, DNA Methylation Assay, Isolation, Quantitative RT-PCR, Mass Spectrometry

    PCR-RFLP analysis of mtDNA cox1 for molecular identification of anisakid larvae. Each PCR product was digested with Hinf I and analyzed on agarose gel. Lanes 1-3, A. pegreffii (each from S. schlegeli , C. myriaster , and H. otakii , respectively); Lanes 4-6, A. simplex s. str. (each from G. macrocephalus , O. masou masou , and O. keta , respectively); Lane M, 100 bp ladder.

    Journal: The Korean Journal of Parasitology

    Article Title: Molecular Analysis of Anisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea

    doi: 10.3347/kjp.2014.52.4.383

    Figure Lengend Snippet: PCR-RFLP analysis of mtDNA cox1 for molecular identification of anisakid larvae. Each PCR product was digested with Hinf I and analyzed on agarose gel. Lanes 1-3, A. pegreffii (each from S. schlegeli , C. myriaster , and H. otakii , respectively); Lanes 4-6, A. simplex s. str. (each from G. macrocephalus , O. masou masou , and O. keta , respectively); Lane M, 100 bp ladder.

    Article Snippet: Amplifications of rDNA ITS and mtDNA cox1 regions Genomic DNA was extracted from individual larva using a Genomic DNA Extraction Kit (Real Biotech Corporation, Banqiao City, Taiwan) according to manufacturer's instruction.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Phylogenetic analysis. (A) rDNA ITS. (B) mtDNA cox1 . Each tree was built by the neighbor-joining method using the MEGA 4 program. The sequences obtained in this study were highlighted with bold letters. Numbers on the branches indicate bootstrap proportions (1,000 replicates).

    Journal: The Korean Journal of Parasitology

    Article Title: Molecular Analysis of Anisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea

    doi: 10.3347/kjp.2014.52.4.383

    Figure Lengend Snippet: Phylogenetic analysis. (A) rDNA ITS. (B) mtDNA cox1 . Each tree was built by the neighbor-joining method using the MEGA 4 program. The sequences obtained in this study were highlighted with bold letters. Numbers on the branches indicate bootstrap proportions (1,000 replicates).

    Article Snippet: Amplifications of rDNA ITS and mtDNA cox1 regions Genomic DNA was extracted from individual larva using a Genomic DNA Extraction Kit (Real Biotech Corporation, Banqiao City, Taiwan) according to manufacturer's instruction.

    Techniques: