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Molecular Research Center inc polymerase chain reaction genomic dna
Polymerase Chain Reaction Genomic Dna, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Retrotransposition-Competent Human LINE-1 Induces Apoptosis in Cancer Cells With Intact p53
Article Snippet: .. Polymerase chain reaction Genomic DNA was extracted using DNAzol reagent (Molecular Research Center, Inc, Ohio). ..

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    Molecular Research Center inc genomic dna isolation total rna
    Identification of circular <t>RNA</t> products in RNA-seq data. ( A ) An RNA-seq cDNA read that maps to the reference <t>DNA</t> in a non-linear, chiastic manner, is a hallmark of circular RNA. ( B ) Schematic representation of the Sulfolobus solfataricus tRNA Trp , which contains a 65 b intron that is cleaved in the process of tRNA maturation and becomes a stable RNA circle. ( C ) Reads derived from the region around the circularization junction of the excised tRNA Trp intron show chiastic mapping to the genome of S. solfataricus P2, exemplifying the power of RNA-seq in circular RNA discovery. Multiple different reads spanning the circularization junction confirm that the observed circular junction is not an amplification artifact. Numbers denote position of the S. solfataricus P2 genome.
    Genomic Dna Isolation Total Rna, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna isolation total rna/product/Molecular Research Center inc
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    88
    Molecular Research Center inc rice genomic dna
    Identification of circular <t>RNA</t> products in RNA-seq data. ( A ) An RNA-seq cDNA read that maps to the reference <t>DNA</t> in a non-linear, chiastic manner, is a hallmark of circular RNA. ( B ) Schematic representation of the Sulfolobus solfataricus tRNA Trp , which contains a 65 b intron that is cleaved in the process of tRNA maturation and becomes a stable RNA circle. ( C ) Reads derived from the region around the circularization junction of the excised tRNA Trp intron show chiastic mapping to the genome of S. solfataricus P2, exemplifying the power of RNA-seq in circular RNA discovery. Multiple different reads spanning the circularization junction confirm that the observed circular junction is not an amplification artifact. Numbers denote position of the S. solfataricus P2 genome.
    Rice Genomic Dna, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rice genomic dna/product/Molecular Research Center inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    88
    Molecular Research Center inc polymerase chain reaction genomic dna
    Identification of circular <t>RNA</t> products in RNA-seq data. ( A ) An RNA-seq cDNA read that maps to the reference <t>DNA</t> in a non-linear, chiastic manner, is a hallmark of circular RNA. ( B ) Schematic representation of the Sulfolobus solfataricus tRNA Trp , which contains a 65 b intron that is cleaved in the process of tRNA maturation and becomes a stable RNA circle. ( C ) Reads derived from the region around the circularization junction of the excised tRNA Trp intron show chiastic mapping to the genome of S. solfataricus P2, exemplifying the power of RNA-seq in circular RNA discovery. Multiple different reads spanning the circularization junction confirm that the observed circular junction is not an amplification artifact. Numbers denote position of the S. solfataricus P2 genome.
    Polymerase Chain Reaction Genomic Dna, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction genomic dna/product/Molecular Research Center inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction genomic dna - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    85
    Molecular Research Center inc genome sequencing viral genomic dna
    Identification of circular <t>RNA</t> products in RNA-seq data. ( A ) An RNA-seq cDNA read that maps to the reference <t>DNA</t> in a non-linear, chiastic manner, is a hallmark of circular RNA. ( B ) Schematic representation of the Sulfolobus solfataricus tRNA Trp , which contains a 65 b intron that is cleaved in the process of tRNA maturation and becomes a stable RNA circle. ( C ) Reads derived from the region around the circularization junction of the excised tRNA Trp intron show chiastic mapping to the genome of S. solfataricus P2, exemplifying the power of RNA-seq in circular RNA discovery. Multiple different reads spanning the circularization junction confirm that the observed circular junction is not an amplification artifact. Numbers denote position of the S. solfataricus P2 genome.
    Genome Sequencing Viral Genomic Dna, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Identification of circular RNA products in RNA-seq data. ( A ) An RNA-seq cDNA read that maps to the reference DNA in a non-linear, chiastic manner, is a hallmark of circular RNA. ( B ) Schematic representation of the Sulfolobus solfataricus tRNA Trp , which contains a 65 b intron that is cleaved in the process of tRNA maturation and becomes a stable RNA circle. ( C ) Reads derived from the region around the circularization junction of the excised tRNA Trp intron show chiastic mapping to the genome of S. solfataricus P2, exemplifying the power of RNA-seq in circular RNA discovery. Multiple different reads spanning the circularization junction confirm that the observed circular junction is not an amplification artifact. Numbers denote position of the S. solfataricus P2 genome.

    Journal: Nucleic Acids Research

    Article Title: Transcriptome-wide discovery of circular RNAs in Archaea

    doi: 10.1093/nar/gkr1009

    Figure Lengend Snippet: Identification of circular RNA products in RNA-seq data. ( A ) An RNA-seq cDNA read that maps to the reference DNA in a non-linear, chiastic manner, is a hallmark of circular RNA. ( B ) Schematic representation of the Sulfolobus solfataricus tRNA Trp , which contains a 65 b intron that is cleaved in the process of tRNA maturation and becomes a stable RNA circle. ( C ) Reads derived from the region around the circularization junction of the excised tRNA Trp intron show chiastic mapping to the genome of S. solfataricus P2, exemplifying the power of RNA-seq in circular RNA discovery. Multiple different reads spanning the circularization junction confirm that the observed circular junction is not an amplification artifact. Numbers denote position of the S. solfataricus P2 genome.

    Article Snippet: Total RNA and genomic DNA isolation Total RNA and DNA samples were isolated from each archaeal cells sample (∼107 cells) using TRI reagent according to the manufacturer's protocol (Molecular Research Center Inc.).

    Techniques: RNA Sequencing Assay, Derivative Assay, Amplification

    Experimental verification of cRNAs by RT-PCR. ( A ) Left, RT–PCR results of amplification with outward-directed primers, designed to amplify cRNA; right, RT–PCR results of amplification with inward-directed primers, expected to amplify both linear and cRNA form. Single and multiple arrowheads represent single or double/triple size products, respectively. N/S, non-specific amplification (as verified by direct sequencing). RT–PCR with each primer set was performed on total RNA sample, RNase R-treated sample, and DNA sample, all extracted from S. solfataricus grown to stationary phase on organotrophic medium. ( B ) RT–PCR for verification of circular RNAs. Arrows indicate outward facing primers (top) and inward facing primers (bottom). Purple line/circle denotes the RNA template, pink line denotes expected PCR product. ( C ) Double and triple sized products can stem from multiple rounds of RT around a circular RNA template, followed by PCR amplification. Arrows mark illustrative PCR primers. ( D ) Northern blot analyses of two ncRNAs with circular forms: (M) Size marker, ( 1 ) ncRNA found in genomic location 1 275 500–1 275 567, ( 2 ) ncRNA found in genomic location 442 786–442 854. Circular forms are indicated by ‘C’, and linear forms by ‘L’.

    Journal: Nucleic Acids Research

    Article Title: Transcriptome-wide discovery of circular RNAs in Archaea

    doi: 10.1093/nar/gkr1009

    Figure Lengend Snippet: Experimental verification of cRNAs by RT-PCR. ( A ) Left, RT–PCR results of amplification with outward-directed primers, designed to amplify cRNA; right, RT–PCR results of amplification with inward-directed primers, expected to amplify both linear and cRNA form. Single and multiple arrowheads represent single or double/triple size products, respectively. N/S, non-specific amplification (as verified by direct sequencing). RT–PCR with each primer set was performed on total RNA sample, RNase R-treated sample, and DNA sample, all extracted from S. solfataricus grown to stationary phase on organotrophic medium. ( B ) RT–PCR for verification of circular RNAs. Arrows indicate outward facing primers (top) and inward facing primers (bottom). Purple line/circle denotes the RNA template, pink line denotes expected PCR product. ( C ) Double and triple sized products can stem from multiple rounds of RT around a circular RNA template, followed by PCR amplification. Arrows mark illustrative PCR primers. ( D ) Northern blot analyses of two ncRNAs with circular forms: (M) Size marker, ( 1 ) ncRNA found in genomic location 1 275 500–1 275 567, ( 2 ) ncRNA found in genomic location 442 786–442 854. Circular forms are indicated by ‘C’, and linear forms by ‘L’.

    Article Snippet: Total RNA and genomic DNA isolation Total RNA and DNA samples were isolated from each archaeal cells sample (∼107 cells) using TRI reagent according to the manufacturer's protocol (Molecular Research Center Inc.).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Sequencing, Polymerase Chain Reaction, Northern Blot, Marker