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Axygen polymerase chain reaction fragments
Polymerase Chain Reaction Fragments, supplied by Axygen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction fragments/product/Axygen
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction fragments - by Bioz Stars, 2020-07
93/100 stars

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Related Articles

Clone Assay:

Article Title: Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized
Article Snippet: .. Polymerase chain reaction conditions were as follows 94°C 3 min, 30× (94°C 1 min, 55°C 1 min, 72°C 1 min), and 72°C 10 min. Polymerase chain reaction fragments were purified from a 1% agarose gel using the AxyPrep DNA gel extraction kit (Axygen Biosciences) and cloned with the pGEM® -T Vector cloning system (Promega) using JM109 Escherichia coli high-efficiency competent cells (Promega), following manufacturer’s instructions. .. One positive clone of each repetitive element was sequenced with a 3500 Genetic Analyzer Sanger sequencing platform at the Biosciences Center of the Federal University of Pernambuco for confirming its identity.

Article Title: IL-12 Expands and Differentiates Human Vγ2Vδ2 T Effector Cells Producing Antimicrobial Cytokines and Inhibiting Intracellular Mycobacterial Growth
Article Snippet: .. The PCR fragments were purified from PCR system by PCR products purification kit (Axygen), and then ligated into the pMD-19T cloning vector (Takara). .. The recombinant plasmids were transfected by heat shock into DH5α competent cells (Sanyou Biotech).

Amplification:

Article Title: Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis
Article Snippet: .. Amplicon purification and high-throughput sequencing After electrophoresis, PCR fragments were extracted from 2% agarose gels using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturer’s instructions. .. The purified DNA fragments were quantified using QuantiFluor: trademark: -ST (Promega, U.S.).

Agarose Gel Electrophoresis:

Article Title: Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized
Article Snippet: .. Polymerase chain reaction conditions were as follows 94°C 3 min, 30× (94°C 1 min, 55°C 1 min, 72°C 1 min), and 72°C 10 min. Polymerase chain reaction fragments were purified from a 1% agarose gel using the AxyPrep DNA gel extraction kit (Axygen Biosciences) and cloned with the pGEM® -T Vector cloning system (Promega) using JM109 Escherichia coli high-efficiency competent cells (Promega), following manufacturer’s instructions. .. One positive clone of each repetitive element was sequenced with a 3500 Genetic Analyzer Sanger sequencing platform at the Biosciences Center of the Federal University of Pernambuco for confirming its identity.

Article Title: Halophilic Archaea Cultivated from Surface Sterilized Middle-Late Eocene Rock Salt Are Polyploid
Article Snippet: .. The PCR fragments were purified by using preparative agarose gel electrophoresis and a gel extraction kit (Axygen Biosciences). .. DNA concentrations were determined photometrically using a photometer (ND-1000, Nanodrop Tech., Rockland, USA).

Polymerase Chain Reaction:

Article Title: Antenna-Specific Glutathione S-Transferase in Male Silkmoth Bombyx mori
Article Snippet: .. PCR fragments were purified using PCR cleanup kits (AxyGen, Union, CA, USA) and subcloned into p28 vector (derived from pET28a, Novagen, Darmstadt, Germany). .. Recombinant clones were validated by restriction digestion and sequenced in both directions.

Article Title: Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized
Article Snippet: .. Polymerase chain reaction conditions were as follows 94°C 3 min, 30× (94°C 1 min, 55°C 1 min, 72°C 1 min), and 72°C 10 min. Polymerase chain reaction fragments were purified from a 1% agarose gel using the AxyPrep DNA gel extraction kit (Axygen Biosciences) and cloned with the pGEM® -T Vector cloning system (Promega) using JM109 Escherichia coli high-efficiency competent cells (Promega), following manufacturer’s instructions. .. One positive clone of each repetitive element was sequenced with a 3500 Genetic Analyzer Sanger sequencing platform at the Biosciences Center of the Federal University of Pernambuco for confirming its identity.

Article Title: IL-12 Expands and Differentiates Human Vγ2Vδ2 T Effector Cells Producing Antimicrobial Cytokines and Inhibiting Intracellular Mycobacterial Growth
Article Snippet: .. The PCR fragments were purified from PCR system by PCR products purification kit (Axygen), and then ligated into the pMD-19T cloning vector (Takara). .. The recombinant plasmids were transfected by heat shock into DH5α competent cells (Sanyou Biotech).

Article Title: Internalin profiling and multilocus sequence typing suggest four Listeria innocua subgroups with different evolutionary distances from Listeria monocytogenes
Article Snippet: .. For DNA sequencing analysis, PCR fragments were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Inc., USA) and their sequences determined by dideoxy method on ABI-PRISM 377 DNA sequencer. .. Internalin profiling By sequence comparison of L. monocytogenes strains F2365, H7858 (serovar 4b), EGDe and F6854 (serovar 1/2a) and L. innocua strain CLIP11262, we investigated the presence or absence of 14 L. monocytogenes-L. innocua -common and 4 L. innocua -specific internalin genes as well as 19 L. monocytogenes -specific internalin genes by PCR with specific primers outlined in Additional file ; table S1.

Article Title: Halophilic Archaea Cultivated from Surface Sterilized Middle-Late Eocene Rock Salt Are Polyploid
Article Snippet: .. The PCR fragments were purified by using preparative agarose gel electrophoresis and a gel extraction kit (Axygen Biosciences). .. DNA concentrations were determined photometrically using a photometer (ND-1000, Nanodrop Tech., Rockland, USA).

Article Title: Aminopeptidase T of M29 Family Acts as A Novel Intracellular Virulence Factor for Listeria monocytogenes Infection
Article Snippet: .. PCR fragments were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Inc., US) and digested with defined restriction enzymes (NEB, Ipswich, US) to facilitate the insertion into vectors. .. Positive clones were then sequenced by GENEWIZ Inc. to verify presence of any target mutations.

Article Title: Do Cryptic Species Exist in Hoplobatrachus rugulosus? An Examination Using Four Nuclear Genes, the Cyt b Gene and the Complete MT Genome
Article Snippet: .. The resultant PCR fragments were electrophoresed on 1% agarose gels, and all target DNAs were purified from excised pieces of gel using an AxyPrep DNA Gel Extraction Kit (Axygen Scientific, Inc. SF, CA, USA) for sequencing. .. The sequence for each fragment was obtained in an automated DNA sequencer (ABI 3730) from both strands.

Article Title: Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis
Article Snippet: .. Amplicon purification and high-throughput sequencing After electrophoresis, PCR fragments were extracted from 2% agarose gels using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturer’s instructions. .. The purified DNA fragments were quantified using QuantiFluor: trademark: -ST (Promega, U.S.).

Purification:

Article Title: Antenna-Specific Glutathione S-Transferase in Male Silkmoth Bombyx mori
Article Snippet: .. PCR fragments were purified using PCR cleanup kits (AxyGen, Union, CA, USA) and subcloned into p28 vector (derived from pET28a, Novagen, Darmstadt, Germany). .. Recombinant clones were validated by restriction digestion and sequenced in both directions.

Article Title: Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized
Article Snippet: .. Polymerase chain reaction conditions were as follows 94°C 3 min, 30× (94°C 1 min, 55°C 1 min, 72°C 1 min), and 72°C 10 min. Polymerase chain reaction fragments were purified from a 1% agarose gel using the AxyPrep DNA gel extraction kit (Axygen Biosciences) and cloned with the pGEM® -T Vector cloning system (Promega) using JM109 Escherichia coli high-efficiency competent cells (Promega), following manufacturer’s instructions. .. One positive clone of each repetitive element was sequenced with a 3500 Genetic Analyzer Sanger sequencing platform at the Biosciences Center of the Federal University of Pernambuco for confirming its identity.

Article Title: IL-12 Expands and Differentiates Human Vγ2Vδ2 T Effector Cells Producing Antimicrobial Cytokines and Inhibiting Intracellular Mycobacterial Growth
Article Snippet: .. The PCR fragments were purified from PCR system by PCR products purification kit (Axygen), and then ligated into the pMD-19T cloning vector (Takara). .. The recombinant plasmids were transfected by heat shock into DH5α competent cells (Sanyou Biotech).

Article Title: Internalin profiling and multilocus sequence typing suggest four Listeria innocua subgroups with different evolutionary distances from Listeria monocytogenes
Article Snippet: .. For DNA sequencing analysis, PCR fragments were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Inc., USA) and their sequences determined by dideoxy method on ABI-PRISM 377 DNA sequencer. .. Internalin profiling By sequence comparison of L. monocytogenes strains F2365, H7858 (serovar 4b), EGDe and F6854 (serovar 1/2a) and L. innocua strain CLIP11262, we investigated the presence or absence of 14 L. monocytogenes-L. innocua -common and 4 L. innocua -specific internalin genes as well as 19 L. monocytogenes -specific internalin genes by PCR with specific primers outlined in Additional file ; table S1.

Article Title: Halophilic Archaea Cultivated from Surface Sterilized Middle-Late Eocene Rock Salt Are Polyploid
Article Snippet: .. The PCR fragments were purified by using preparative agarose gel electrophoresis and a gel extraction kit (Axygen Biosciences). .. DNA concentrations were determined photometrically using a photometer (ND-1000, Nanodrop Tech., Rockland, USA).

Article Title: Aminopeptidase T of M29 Family Acts as A Novel Intracellular Virulence Factor for Listeria monocytogenes Infection
Article Snippet: .. PCR fragments were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Inc., US) and digested with defined restriction enzymes (NEB, Ipswich, US) to facilitate the insertion into vectors. .. Positive clones were then sequenced by GENEWIZ Inc. to verify presence of any target mutations.

Article Title: Do Cryptic Species Exist in Hoplobatrachus rugulosus? An Examination Using Four Nuclear Genes, the Cyt b Gene and the Complete MT Genome
Article Snippet: .. The resultant PCR fragments were electrophoresed on 1% agarose gels, and all target DNAs were purified from excised pieces of gel using an AxyPrep DNA Gel Extraction Kit (Axygen Scientific, Inc. SF, CA, USA) for sequencing. .. The sequence for each fragment was obtained in an automated DNA sequencer (ABI 3730) from both strands.

Article Title: Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis
Article Snippet: .. Amplicon purification and high-throughput sequencing After electrophoresis, PCR fragments were extracted from 2% agarose gels using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturer’s instructions. .. The purified DNA fragments were quantified using QuantiFluor: trademark: -ST (Promega, U.S.).

Electrophoresis:

Article Title: Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis
Article Snippet: .. Amplicon purification and high-throughput sequencing After electrophoresis, PCR fragments were extracted from 2% agarose gels using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturer’s instructions. .. The purified DNA fragments were quantified using QuantiFluor: trademark: -ST (Promega, U.S.).

Sequencing:

Article Title: Do Cryptic Species Exist in Hoplobatrachus rugulosus? An Examination Using Four Nuclear Genes, the Cyt b Gene and the Complete MT Genome
Article Snippet: .. The resultant PCR fragments were electrophoresed on 1% agarose gels, and all target DNAs were purified from excised pieces of gel using an AxyPrep DNA Gel Extraction Kit (Axygen Scientific, Inc. SF, CA, USA) for sequencing. .. The sequence for each fragment was obtained in an automated DNA sequencer (ABI 3730) from both strands.

Article Title: Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis
Article Snippet: .. Amplicon purification and high-throughput sequencing After electrophoresis, PCR fragments were extracted from 2% agarose gels using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturer’s instructions. .. The purified DNA fragments were quantified using QuantiFluor: trademark: -ST (Promega, U.S.).

Derivative Assay:

Article Title: Antenna-Specific Glutathione S-Transferase in Male Silkmoth Bombyx mori
Article Snippet: .. PCR fragments were purified using PCR cleanup kits (AxyGen, Union, CA, USA) and subcloned into p28 vector (derived from pET28a, Novagen, Darmstadt, Germany). .. Recombinant clones were validated by restriction digestion and sequenced in both directions.

High Throughput Screening Assay:

Article Title: Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis
Article Snippet: .. Amplicon purification and high-throughput sequencing After electrophoresis, PCR fragments were extracted from 2% agarose gels using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturer’s instructions. .. The purified DNA fragments were quantified using QuantiFluor: trademark: -ST (Promega, U.S.).

Gel Extraction:

Article Title: Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized
Article Snippet: .. Polymerase chain reaction conditions were as follows 94°C 3 min, 30× (94°C 1 min, 55°C 1 min, 72°C 1 min), and 72°C 10 min. Polymerase chain reaction fragments were purified from a 1% agarose gel using the AxyPrep DNA gel extraction kit (Axygen Biosciences) and cloned with the pGEM® -T Vector cloning system (Promega) using JM109 Escherichia coli high-efficiency competent cells (Promega), following manufacturer’s instructions. .. One positive clone of each repetitive element was sequenced with a 3500 Genetic Analyzer Sanger sequencing platform at the Biosciences Center of the Federal University of Pernambuco for confirming its identity.

Article Title: Internalin profiling and multilocus sequence typing suggest four Listeria innocua subgroups with different evolutionary distances from Listeria monocytogenes
Article Snippet: .. For DNA sequencing analysis, PCR fragments were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Inc., USA) and their sequences determined by dideoxy method on ABI-PRISM 377 DNA sequencer. .. Internalin profiling By sequence comparison of L. monocytogenes strains F2365, H7858 (serovar 4b), EGDe and F6854 (serovar 1/2a) and L. innocua strain CLIP11262, we investigated the presence or absence of 14 L. monocytogenes-L. innocua -common and 4 L. innocua -specific internalin genes as well as 19 L. monocytogenes -specific internalin genes by PCR with specific primers outlined in Additional file ; table S1.

Article Title: Halophilic Archaea Cultivated from Surface Sterilized Middle-Late Eocene Rock Salt Are Polyploid
Article Snippet: .. The PCR fragments were purified by using preparative agarose gel electrophoresis and a gel extraction kit (Axygen Biosciences). .. DNA concentrations were determined photometrically using a photometer (ND-1000, Nanodrop Tech., Rockland, USA).

Article Title: Aminopeptidase T of M29 Family Acts as A Novel Intracellular Virulence Factor for Listeria monocytogenes Infection
Article Snippet: .. PCR fragments were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Inc., US) and digested with defined restriction enzymes (NEB, Ipswich, US) to facilitate the insertion into vectors. .. Positive clones were then sequenced by GENEWIZ Inc. to verify presence of any target mutations.

Article Title: Do Cryptic Species Exist in Hoplobatrachus rugulosus? An Examination Using Four Nuclear Genes, the Cyt b Gene and the Complete MT Genome
Article Snippet: .. The resultant PCR fragments were electrophoresed on 1% agarose gels, and all target DNAs were purified from excised pieces of gel using an AxyPrep DNA Gel Extraction Kit (Axygen Scientific, Inc. SF, CA, USA) for sequencing. .. The sequence for each fragment was obtained in an automated DNA sequencer (ABI 3730) from both strands.

Article Title: Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis
Article Snippet: .. Amplicon purification and high-throughput sequencing After electrophoresis, PCR fragments were extracted from 2% agarose gels using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturer’s instructions. .. The purified DNA fragments were quantified using QuantiFluor: trademark: -ST (Promega, U.S.).

Plasmid Preparation:

Article Title: Antenna-Specific Glutathione S-Transferase in Male Silkmoth Bombyx mori
Article Snippet: .. PCR fragments were purified using PCR cleanup kits (AxyGen, Union, CA, USA) and subcloned into p28 vector (derived from pET28a, Novagen, Darmstadt, Germany). .. Recombinant clones were validated by restriction digestion and sequenced in both directions.

Article Title: Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized
Article Snippet: .. Polymerase chain reaction conditions were as follows 94°C 3 min, 30× (94°C 1 min, 55°C 1 min, 72°C 1 min), and 72°C 10 min. Polymerase chain reaction fragments were purified from a 1% agarose gel using the AxyPrep DNA gel extraction kit (Axygen Biosciences) and cloned with the pGEM® -T Vector cloning system (Promega) using JM109 Escherichia coli high-efficiency competent cells (Promega), following manufacturer’s instructions. .. One positive clone of each repetitive element was sequenced with a 3500 Genetic Analyzer Sanger sequencing platform at the Biosciences Center of the Federal University of Pernambuco for confirming its identity.

Article Title: IL-12 Expands and Differentiates Human Vγ2Vδ2 T Effector Cells Producing Antimicrobial Cytokines and Inhibiting Intracellular Mycobacterial Growth
Article Snippet: .. The PCR fragments were purified from PCR system by PCR products purification kit (Axygen), and then ligated into the pMD-19T cloning vector (Takara). .. The recombinant plasmids were transfected by heat shock into DH5α competent cells (Sanyou Biotech).

DNA Sequencing:

Article Title: Internalin profiling and multilocus sequence typing suggest four Listeria innocua subgroups with different evolutionary distances from Listeria monocytogenes
Article Snippet: .. For DNA sequencing analysis, PCR fragments were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Inc., USA) and their sequences determined by dideoxy method on ABI-PRISM 377 DNA sequencer. .. Internalin profiling By sequence comparison of L. monocytogenes strains F2365, H7858 (serovar 4b), EGDe and F6854 (serovar 1/2a) and L. innocua strain CLIP11262, we investigated the presence or absence of 14 L. monocytogenes-L. innocua -common and 4 L. innocua -specific internalin genes as well as 19 L. monocytogenes -specific internalin genes by PCR with specific primers outlined in Additional file ; table S1.

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  • 85
    Axygen target 16s rrna gene fragments
    Comparison of similarities and differences for gut microbiota composition between transgenic fish and wild-type controls across different developmental stages. (a) Comparison of the average Sørensen index obtained from DGGE patterns of <t>16S</t> <t>rRNA</t> genes between transgenic fish and wild-type controls or within each group of transgenic fish and wild-type controls. (b) Comparison of Raup and Crick similarity index ( S RC ) obtained from DGGE patterns of 16S rRNA genes between transgenic fish and wild-type controls or within each group of transgenic fish and wild-type controls. Dashed lines indicate significant cutoff for difference (low line) and similarity (upper line). Error bars represent the standard error of the mean.
    Target 16s Rrna Gene Fragments, supplied by Axygen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/target 16s rrna gene fragments/product/Axygen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    target 16s rrna gene fragments - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    88
    Axygen pcr fragment insert
    <t>PCR</t> product agarose gel electrophoresis for the identification of S. aureus ATCC25923 agr genotype. Whole- cell PCR was performed with each of the four agr specificity primers. Lines 1 to 4, PCR product using the same forward primer pan- agr and different reverse primers ( agr I, agr II, agr III, and agr IV) with S. aureus ATCC25923 genomic <t>DNA</t> as templates (439 bp for agr I, 573 bp for agr II, 406 bp for agr III, and 657 bp for agr IV); Lines 5 to 8, negative controls responsible to lines 1 to 4, respectively; Lines M, 100 bp for DNA ladder.
    Pcr Fragment Insert, supplied by Axygen, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr fragment insert/product/Axygen
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr fragment insert - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    94
    Axygen pcr cleanup kit
    Generation of ZFN-mediated c-kit mutant mice. a. Schematic showing the layout of ZFN target sites. The left and right ZFN binding sites with the spacer region are shown. b. Sequence analysis of the modified c-kit allele in 44 founder animals. The targeted region on the mouse c-kit locus was <t>PCR-amplified</t> from genomic <t>DNA</t> extracted from tails of the 5-day-old F0 pups, then subjected to T-A cloning. Colonies were randomly picked for DNA sequencing. Indels and mutations were detected in 8 out of 27 founders treated with 2.5 ng/μl ZFN mRNA and 7 out of 17 founders treated with 5 ng/μl ZFN mRNA. The ZFN binding site is underlined. Indels and mutations are highlighted in red.
    Pcr Cleanup Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 94/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr cleanup kit/product/Axygen
    Average 94 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    pcr cleanup kit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    91
    Axygen pcr dna fragments
    Sensitivity assessment of loop-mediated isothermal amplification (LAMP) assay and conventional <t>PCR</t> for detection of Tylenchulus semipenetrans . (A) Visual examination of LAMP products by adding SYBR Green I fluorescence dye. (B) Agarose gel electrophoresis of LAMP products. (C) Agarose gel electrophoresis of conventional PCR products. Tubes and lanes 1–9, genomic <t>DNA</t> from 100, 10, 1, 10 −1 , 10 −2 , 10 −3 , 10 −4 , 10 −5 , 10 −6 J2/0.5 g of soil, respectively; tube and lane 10, no template control; lane M, molecular marker.
    Pcr Dna Fragments, supplied by Axygen, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr dna fragments/product/Axygen
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr dna fragments - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of similarities and differences for gut microbiota composition between transgenic fish and wild-type controls across different developmental stages. (a) Comparison of the average Sørensen index obtained from DGGE patterns of 16S rRNA genes between transgenic fish and wild-type controls or within each group of transgenic fish and wild-type controls. (b) Comparison of Raup and Crick similarity index ( S RC ) obtained from DGGE patterns of 16S rRNA genes between transgenic fish and wild-type controls or within each group of transgenic fish and wild-type controls. Dashed lines indicate significant cutoff for difference (low line) and similarity (upper line). Error bars represent the standard error of the mean.

    Journal: PLoS ONE

    Article Title: Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.)

    doi: 10.1371/journal.pone.0064577

    Figure Lengend Snippet: Comparison of similarities and differences for gut microbiota composition between transgenic fish and wild-type controls across different developmental stages. (a) Comparison of the average Sørensen index obtained from DGGE patterns of 16S rRNA genes between transgenic fish and wild-type controls or within each group of transgenic fish and wild-type controls. (b) Comparison of Raup and Crick similarity index ( S RC ) obtained from DGGE patterns of 16S rRNA genes between transgenic fish and wild-type controls or within each group of transgenic fish and wild-type controls. Dashed lines indicate significant cutoff for difference (low line) and similarity (upper line). Error bars represent the standard error of the mean.

    Article Snippet: Target 16S rRNA gene fragments were excised and purified using agarose gel DNA extraction kit (Axygen), cloned into pMD18-T vector (TaKaRa), and then transformed Escherichia coli (DH-5a) with a plasmid.

    Techniques: Transgenic Assay, Fluorescence In Situ Hybridization, Denaturing Gradient Gel Electrophoresis

    Comparison of relative Bacteroidetes and Firmicutes abundance at different developmental stages. Real-time quantitative PCR (Q-PCR) was used to quantify the abundance of gut Firmicutes and Bacteroidetes based on the 16S rRNA genes (V3 region). (a) Relative abundance of Firmicutes and Bacteroidetes in transgenic fish. (b) Relative abundance of Firmicutes and Bacteroidetes in wild-type controls.

    Journal: PLoS ONE

    Article Title: Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.)

    doi: 10.1371/journal.pone.0064577

    Figure Lengend Snippet: Comparison of relative Bacteroidetes and Firmicutes abundance at different developmental stages. Real-time quantitative PCR (Q-PCR) was used to quantify the abundance of gut Firmicutes and Bacteroidetes based on the 16S rRNA genes (V3 region). (a) Relative abundance of Firmicutes and Bacteroidetes in transgenic fish. (b) Relative abundance of Firmicutes and Bacteroidetes in wild-type controls.

    Article Snippet: Target 16S rRNA gene fragments were excised and purified using agarose gel DNA extraction kit (Axygen), cloned into pMD18-T vector (TaKaRa), and then transformed Escherichia coli (DH-5a) with a plasmid.

    Techniques: Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Transgenic Assay, Fluorescence In Situ Hybridization

    PCR product agarose gel electrophoresis for the identification of S. aureus ATCC25923 agr genotype. Whole- cell PCR was performed with each of the four agr specificity primers. Lines 1 to 4, PCR product using the same forward primer pan- agr and different reverse primers ( agr I, agr II, agr III, and agr IV) with S. aureus ATCC25923 genomic DNA as templates (439 bp for agr I, 573 bp for agr II, 406 bp for agr III, and 657 bp for agr IV); Lines 5 to 8, negative controls responsible to lines 1 to 4, respectively; Lines M, 100 bp for DNA ladder.

    Journal: Scientific Reports

    Article Title: Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus

    doi: 10.1038/srep11997

    Figure Lengend Snippet: PCR product agarose gel electrophoresis for the identification of S. aureus ATCC25923 agr genotype. Whole- cell PCR was performed with each of the four agr specificity primers. Lines 1 to 4, PCR product using the same forward primer pan- agr and different reverse primers ( agr I, agr II, agr III, and agr IV) with S. aureus ATCC25923 genomic DNA as templates (439 bp for agr I, 573 bp for agr II, 406 bp for agr III, and 657 bp for agr IV); Lines 5 to 8, negative controls responsible to lines 1 to 4, respectively; Lines M, 100 bp for DNA ladder.

    Article Snippet: The plasmid DNA containing the PCR fragment insert was isolated from each culture using AxyPrep Plasmid Miniprep kit (Axygen, USA) according to manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Generation of ZFN-mediated c-kit mutant mice. a. Schematic showing the layout of ZFN target sites. The left and right ZFN binding sites with the spacer region are shown. b. Sequence analysis of the modified c-kit allele in 44 founder animals. The targeted region on the mouse c-kit locus was PCR-amplified from genomic DNA extracted from tails of the 5-day-old F0 pups, then subjected to T-A cloning. Colonies were randomly picked for DNA sequencing. Indels and mutations were detected in 8 out of 27 founders treated with 2.5 ng/μl ZFN mRNA and 7 out of 17 founders treated with 5 ng/μl ZFN mRNA. The ZFN binding site is underlined. Indels and mutations are highlighted in red.

    Journal: PLoS ONE

    Article Title: Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes

    doi: 10.1371/journal.pone.0077696

    Figure Lengend Snippet: Generation of ZFN-mediated c-kit mutant mice. a. Schematic showing the layout of ZFN target sites. The left and right ZFN binding sites with the spacer region are shown. b. Sequence analysis of the modified c-kit allele in 44 founder animals. The targeted region on the mouse c-kit locus was PCR-amplified from genomic DNA extracted from tails of the 5-day-old F0 pups, then subjected to T-A cloning. Colonies were randomly picked for DNA sequencing. Indels and mutations were detected in 8 out of 27 founders treated with 2.5 ng/μl ZFN mRNA and 7 out of 17 founders treated with 5 ng/μl ZFN mRNA. The ZFN binding site is underlined. Indels and mutations are highlighted in red.

    Article Snippet: In brief, targeted fragments were amplified from extracted DNA, and purified using a PCR cleanup kit (Axygen, AP-PCR-50).

    Techniques: Mutagenesis, Mouse Assay, Binding Assay, Sequencing, Modification, Polymerase Chain Reaction, Amplification, Clone Assay, DNA Sequencing

    Targeted integration of a loxP site using ZFNc-kit and gene-targeting ssODNs . a. A schematic of the mouse c-kit locus and the ssODN sequences used to introduce a loxP site (in blue) into the genome in vivo . b. The representative gel electropherogram of the genotyping results by PCR amplification. 5 day old pups were genotyped by PCR amplification of tail genomic DNA. The expected wild type band is 178 bp (WT) and the expected mutant band is 214 bp. The PCR primers are listed in Table S1 . Ng, negative control. c. Sequence analysis of the mutant c-kit allele of the 9 founders. The target region of the mouse c-kit gene was PCR-amplified from tail genomic DNA of the 5-day-old founders and subjected to T-A cloning. Colonies were randomly picked for DNA sequencing. Precise loxP site insertion was detected in 2 out of 9 founders. ZFN binding site is underlined. The loxP sites are highlighted in blue. Indels are highlighted in red. WT, wild type.

    Journal: PLoS ONE

    Article Title: Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes

    doi: 10.1371/journal.pone.0077696

    Figure Lengend Snippet: Targeted integration of a loxP site using ZFNc-kit and gene-targeting ssODNs . a. A schematic of the mouse c-kit locus and the ssODN sequences used to introduce a loxP site (in blue) into the genome in vivo . b. The representative gel electropherogram of the genotyping results by PCR amplification. 5 day old pups were genotyped by PCR amplification of tail genomic DNA. The expected wild type band is 178 bp (WT) and the expected mutant band is 214 bp. The PCR primers are listed in Table S1 . Ng, negative control. c. Sequence analysis of the mutant c-kit allele of the 9 founders. The target region of the mouse c-kit gene was PCR-amplified from tail genomic DNA of the 5-day-old founders and subjected to T-A cloning. Colonies were randomly picked for DNA sequencing. Precise loxP site insertion was detected in 2 out of 9 founders. ZFN binding site is underlined. The loxP sites are highlighted in blue. Indels are highlighted in red. WT, wild type.

    Article Snippet: In brief, targeted fragments were amplified from extracted DNA, and purified using a PCR cleanup kit (Axygen, AP-PCR-50).

    Techniques: Introduce, In Vivo, Polymerase Chain Reaction, Amplification, Mutagenesis, Negative Control, Sequencing, Clone Assay, DNA Sequencing, Binding Assay

    Targeted insertion of a restriction site using ZFNc-kit and gene-targeting ssODNs. a. A schematic of the mouse c-kit locus and the ssODN sequences of different sizes designed to introduce an exogenous BglII restriction site (in blue) into the genome in vivo using ZFNc-kit and ssODNs. b. Detection of introduced BglII site in founder animals. The representative gel electropherogram of genotyping results by BglII digestion of PCR product. F0 pups were genotyped by BglII digestion of the 592 bp PCR product amplified from tail genomic DNA. The PCR product from the wild type c-kit allele cannot be digested by BglII (WT), while the PCR product from the targeted mutant allele (10 out of 109 founders) can be digested by BglII into two distinct fragments (238 bp and 354 bp). The PCR primers are listed in Table S1 . c. Sequence analysis of the c-kit mutant allele of the 10 founders with correct BglII restriction bands. The target region of the mouse c-kit gene was PCR-amplified from tail genomic DNA of the 5-day-old founders and subjected to T-A cloning. Colonies were randomly picked for DNA sequencing. Precise BglII site insertion was detected in 8 out of 10 mutant founders. ZFN binding site is underlined. BglII sites are highlighted in blue. Indels are highlighted in red.

    Journal: PLoS ONE

    Article Title: Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes

    doi: 10.1371/journal.pone.0077696

    Figure Lengend Snippet: Targeted insertion of a restriction site using ZFNc-kit and gene-targeting ssODNs. a. A schematic of the mouse c-kit locus and the ssODN sequences of different sizes designed to introduce an exogenous BglII restriction site (in blue) into the genome in vivo using ZFNc-kit and ssODNs. b. Detection of introduced BglII site in founder animals. The representative gel electropherogram of genotyping results by BglII digestion of PCR product. F0 pups were genotyped by BglII digestion of the 592 bp PCR product amplified from tail genomic DNA. The PCR product from the wild type c-kit allele cannot be digested by BglII (WT), while the PCR product from the targeted mutant allele (10 out of 109 founders) can be digested by BglII into two distinct fragments (238 bp and 354 bp). The PCR primers are listed in Table S1 . c. Sequence analysis of the c-kit mutant allele of the 10 founders with correct BglII restriction bands. The target region of the mouse c-kit gene was PCR-amplified from tail genomic DNA of the 5-day-old founders and subjected to T-A cloning. Colonies were randomly picked for DNA sequencing. Precise BglII site insertion was detected in 8 out of 10 mutant founders. ZFN binding site is underlined. BglII sites are highlighted in blue. Indels are highlighted in red.

    Article Snippet: In brief, targeted fragments were amplified from extracted DNA, and purified using a PCR cleanup kit (Axygen, AP-PCR-50).

    Techniques: Introduce, In Vivo, Polymerase Chain Reaction, Amplification, Mutagenesis, Sequencing, Clone Assay, DNA Sequencing, Binding Assay

    Germline transmission of the targeting modification. a. The representative gel electropherogram of the genotyping result by BglII digestion of the PCR product. F1 mice derived from founders C4, #7-1, and A22 were genotyped by BglII digestion of the PCR product (592 bp) amplified from tail genomic DNA of 5-day-old pups. The PCR product from the wild type c-kit allele cannot be digested by BglII (WT), while the targeted mutant allele can be digested by BglII into two distinct fragments (238 bp and 354 bp). The PCR primers are listed in Table S1 . b. Sequence analysis of the BglII restriction site insertion. The F1 mice 1 2 from founder C4, #1 2 from founder 7-1, and #3 8 from founder A22 were selected for sequence analysis to confirm precise restrict site insertion. ZFN binding site is underlined. BglII sites are highlighted in blue. WT, wild type. c. The representative gel electropherogram of the genotyping results by PCR amplification. A total of 13 F1 mice from founder 57 59 with loxP integration were genotyped by PCR amplification using tail genomic DNA from 5-day-old pups. The expected wild type (WT) band is 178 bp, while the expected mutant band is 214 bp. The PCR primers are listed in Table S1 . Ng, negative control. d. Sequence analysis of the mutant c-kit allele of the F1 mice 1, 3, 7, and 8 from founder 57, and #3 4 from founder 59. The targeted region of the mouse c-kit locus was PCR-amplified from tail genomic DNA of 5-day-old founders and subjected to sequencing. Precise loxP integration was detected in F1 mice 1 3 from founder 57, 3 4 from founder 59. ZFN binding site is underlined. loxP sites are highlighted in blue. Deletions are highlighted in red. WT, wild type.

    Journal: PLoS ONE

    Article Title: Efficient Knockin Mouse Generation by ssDNA Oligonucleotides and Zinc-Finger Nuclease Assisted Homologous Recombination in Zygotes

    doi: 10.1371/journal.pone.0077696

    Figure Lengend Snippet: Germline transmission of the targeting modification. a. The representative gel electropherogram of the genotyping result by BglII digestion of the PCR product. F1 mice derived from founders C4, #7-1, and A22 were genotyped by BglII digestion of the PCR product (592 bp) amplified from tail genomic DNA of 5-day-old pups. The PCR product from the wild type c-kit allele cannot be digested by BglII (WT), while the targeted mutant allele can be digested by BglII into two distinct fragments (238 bp and 354 bp). The PCR primers are listed in Table S1 . b. Sequence analysis of the BglII restriction site insertion. The F1 mice 1 2 from founder C4, #1 2 from founder 7-1, and #3 8 from founder A22 were selected for sequence analysis to confirm precise restrict site insertion. ZFN binding site is underlined. BglII sites are highlighted in blue. WT, wild type. c. The representative gel electropherogram of the genotyping results by PCR amplification. A total of 13 F1 mice from founder 57 59 with loxP integration were genotyped by PCR amplification using tail genomic DNA from 5-day-old pups. The expected wild type (WT) band is 178 bp, while the expected mutant band is 214 bp. The PCR primers are listed in Table S1 . Ng, negative control. d. Sequence analysis of the mutant c-kit allele of the F1 mice 1, 3, 7, and 8 from founder 57, and #3 4 from founder 59. The targeted region of the mouse c-kit locus was PCR-amplified from tail genomic DNA of 5-day-old founders and subjected to sequencing. Precise loxP integration was detected in F1 mice 1 3 from founder 57, 3 4 from founder 59. ZFN binding site is underlined. loxP sites are highlighted in blue. Deletions are highlighted in red. WT, wild type.

    Article Snippet: In brief, targeted fragments were amplified from extracted DNA, and purified using a PCR cleanup kit (Axygen, AP-PCR-50).

    Techniques: Transmission Assay, Modification, Polymerase Chain Reaction, Mouse Assay, Derivative Assay, Amplification, Mutagenesis, Sequencing, Binding Assay, Negative Control

    Sensitivity assessment of loop-mediated isothermal amplification (LAMP) assay and conventional PCR for detection of Tylenchulus semipenetrans . (A) Visual examination of LAMP products by adding SYBR Green I fluorescence dye. (B) Agarose gel electrophoresis of LAMP products. (C) Agarose gel electrophoresis of conventional PCR products. Tubes and lanes 1–9, genomic DNA from 100, 10, 1, 10 −1 , 10 −2 , 10 −3 , 10 −4 , 10 −5 , 10 −6 J2/0.5 g of soil, respectively; tube and lane 10, no template control; lane M, molecular marker.

    Journal: The Plant Pathology Journal

    Article Title: Development and Evaluation of Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Tylenchulus semipenetrans Using DNA Extracted from Soil

    doi: 10.5423/PPJ.OA.10.2016.0224

    Figure Lengend Snippet: Sensitivity assessment of loop-mediated isothermal amplification (LAMP) assay and conventional PCR for detection of Tylenchulus semipenetrans . (A) Visual examination of LAMP products by adding SYBR Green I fluorescence dye. (B) Agarose gel electrophoresis of LAMP products. (C) Agarose gel electrophoresis of conventional PCR products. Tubes and lanes 1–9, genomic DNA from 100, 10, 1, 10 −1 , 10 −2 , 10 −3 , 10 −4 , 10 −5 , 10 −6 J2/0.5 g of soil, respectively; tube and lane 10, no template control; lane M, molecular marker.

    Article Snippet: The PCR DNA fragments were extracted from 2% agarose gel using an AxyPrep DNA Gel Extraction Kit (Axygen, Union City, CA, USA).

    Techniques: Amplification, Lamp Assay, Polymerase Chain Reaction, SYBR Green Assay, Fluorescence, Agarose Gel Electrophoresis, Marker