polymerase buffer  (Qiagen)


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    Structured Review

    Qiagen polymerase buffer
    Polymerase Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase buffer/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    polymerase buffer - by Bioz Stars, 2020-02
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    Related Articles

    DNA Extraction:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: Comparable community analyses were obtained regardless of filter type or DNA extraction method utilized (data not shown). .. PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen).

    Irradiation:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen). .. PCR mixtures containing all components except the template and Taq polymerase were UV irradiated for 5 min to ensure sterility.

    Amplification:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: 16S rDNA was amplified with two primer sets: (i) 8F (AGAGTTTGATCMTGGCTCAG) and 519R (GWATTACCGCGGCKGCTG) and (ii) 338F (ACTCCTACGGGAGGCAGC) and 907R (CCGTCAATTCMTTTRAGTTT) ( , , ). .. PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen).

    Agarose Gel Electrophoresis:

    Article Title: The MreB-Like Protein Mbl of Streptomyces coelicolor A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ †
    Article Snippet: PCRs were performed with the Bio-Rad MJ mini personal thermal cycler using 60 ng of cDNA, 400 nM each primer, 200 μM deoxynucleoside triphosphates (dNTPs), 1× polymerase buffer (Qiagen), and 1 U Taq polymerase (Qiagen) in a 25-μl volume. .. The RT-PCR samples were loaded on a 2% agarose gel and stained with ethidium bromide.

    Clone Assay:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen). .. PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen).

    Isolation:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen). .. PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen).

    Article Title: The MreB-Like Protein Mbl of Streptomyces coelicolor A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ †
    Article Snippet: RNA was isolated from two independently grown cultures, and RT-PCR was repeated twice. cDNA synthesis was performed as described in reference using 3 μg of RNA. .. PCRs were performed with the Bio-Rad MJ mini personal thermal cycler using 60 ng of cDNA, 400 nM each primer, 200 μM deoxynucleoside triphosphates (dNTPs), 1× polymerase buffer (Qiagen), and 1 U Taq polymerase (Qiagen) in a 25-μl volume.

    Polymerase Chain Reaction:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: .. PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen). .. PCR mixtures containing all components except the template and Taq polymerase were UV irradiated for 5 min to ensure sterility.

    Article Title: The MreB-Like Protein Mbl of Streptomyces coelicolor A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ †
    Article Snippet: The optimal PCR annealing temperature of all primers was 62°C. .. PCRs were performed with the Bio-Rad MJ mini personal thermal cycler using 60 ng of cDNA, 400 nM each primer, 200 μM deoxynucleoside triphosphates (dNTPs), 1× polymerase buffer (Qiagen), and 1 U Taq polymerase (Qiagen) in a 25-μl volume.

    Modification:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: Briefly, DNA from filters was extracted with either the FastDNA SPIN kit (Bio101, Inc., Carlsbad, Calif.) or by a modified phenol-chloroform DNA extraction method ( , ). .. PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen).

    Gel Extraction:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen). .. PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The MreB-Like Protein Mbl of Streptomyces coelicolor A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ †
    Article Snippet: Paragraph title: Semiquantitative RT-PCR analysis. ... PCRs were performed with the Bio-Rad MJ mini personal thermal cycler using 60 ng of cDNA, 400 nM each primer, 200 μM deoxynucleoside triphosphates (dNTPs), 1× polymerase buffer (Qiagen), and 1 U Taq polymerase (Qiagen) in a 25-μl volume.

    Staining:

    Article Title: The MreB-Like Protein Mbl of Streptomyces coelicolor A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ A3(2) Depends on MreB for Proper Localization and Contributes to Spore Wall Synthesis ▿ †
    Article Snippet: PCRs were performed with the Bio-Rad MJ mini personal thermal cycler using 60 ng of cDNA, 400 nM each primer, 200 μM deoxynucleoside triphosphates (dNTPs), 1× polymerase buffer (Qiagen), and 1 U Taq polymerase (Qiagen) in a 25-μl volume. .. The RT-PCR samples were loaded on a 2% agarose gel and stained with ethidium bromide.

    Sterility:

    Article Title: Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
    Article Snippet: PCR mixtures (100 μl) contained ∼5 ng of template DNA, 10 μl of 10× polymerase buffer (Qiagen, Valencia, Calif.), 20 μl of 5× Q buffer (Qiagen), 3 mM MgCl2 (Qiagen), 1 μl of bovine serum albumin (0.5 mg/ml), 200 μM deoxyribonucleoside triphosphates (Sigma-Aldrich Co., St. Louis, Mo.), 25 pmol of forward and reverse primers (Sigma-Genosys, The Woodlands, Tex.), and 1.25 U of Taq polymerase (Qiagen). .. PCR mixtures containing all components except the template and Taq polymerase were UV irradiated for 5 min to ensure sterility.

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    Qiagen rna later solution
    A, Combined left ventricular wall width (includes both the intraventricular septum and left ventricular free wall), left ventricular free wall only, and intraventricular septum only. Left ventricular free wall data were log transformed and an unpaired Student t test was performed. B, Frozen sections (5 μm) of cardiac tissue were stained with wheat germ agglutinin and imaged for measurements of myocyte width. Consecutive sections of the same representative images were then stained for hematoxylin-and-eosin-stained sections. Scale bar, 50 μm for all images. Only myocytes in longitudinal axis cross-section were measured and widths measured from a total of 80–100 cells from two separate images per sample. Open bars [control (Ctrl)] and filled bars (Mat-Ob). Data are expressed as means ± sem . <t>RNA</t> from ventricular tissue of control and Mat-Ob males at 8 wk of age (n = 7 and 9, respectively, in which one offspring represents one litter) was isolated, purified, and cDNA obtained by reverse transcription. C, Gene expression of Nppb , Nppa , Myh7 , and Myh6 was quantified by real-time <t>PCR</t> using primers shown in Supplemental Table 1. All data were normalized to Gapdh as described in Materials and Methods . The ratio of Mhy7 to Myh6 was analyzed by a Mann-Whitney U test and presented as means ± interquartile ranges (n = 7 control and n = 9 Mat-Ob for all analyses). ***, P
    Rna Later Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen buffer iii
    Chromatin marks at the IL12B locus. (A) A genome browser snapshot, including ChIP-seq tracks for α-H3K4me3, α-H3K4me1, α-H3K9me3, α-H3K27me3, α-C/EBPβ, α-H3K27ac, α-H3K36me3, IgG (unspecific polyclonal rabbit IgG pool) chromatin immunoprecipitations, and MIRA from a TAM sample freshly isolated from ovarian carcinoma ascites. Regions of interest are highlighted by rectangles. (B–D) ChIP-qPCR analyses of the indicated histone marks in an independent TAM sample at the indicated genomic locations amplified by specific primers are indicated. The error bars denote <t>SDs</t> from technical PCR replicates. (E–G) ChIP-qPCR analyses of the indicated histone marks in MDMs from <t>three</t> different pooled donor populations ( N = 3; N = 4 for IgG and α-H3K27me3 samples) differentiated in normal medium, in ascites, or in normal medium followed by ascites for 1 day. Cells were stimulated with or without LPS and IFNγ (+) or their respective solvents (−) 2.5 h prior to harvesting. Genomic regions were amplified by specific primers are indicated. Each dot denotes a biological replicate; for each replicate, MDMs from six donors were pooled after harvesting of the cells for each experiment. Median values are indicated by horizontal bars. Colors encode ascites samples from individual patients, and colors are consistent between panels within this figure, Figure 5 and Figure S9 in Supplementary Material. Statistical significances were calculated with paired t -tests. ** P
    Buffer Iii, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen pcr buffer
    Schematic from <t>PCR</t> gene cloning to Agrobacterium expression clones using the Gateway ® cloning technology. Col E1 ori : origin of replication for E. coli . pVS1 ori: origin of replication for Agrobacteria . Kan r : kanamycin resistance gene. Cm r : chloramphenicol resistance gene. RB: <t>T-DNA</t> right border. LB: T-DNA left border. att L, att R, att B: recombination sites. ccdB: E. coli ccdB gene (note: the ccdB protein interferes with E. coli DNA gyrase and inhibits growth of most E. coli strains, e.g., DH5α™).
    Pcr Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Qiagen
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    A, Combined left ventricular wall width (includes both the intraventricular septum and left ventricular free wall), left ventricular free wall only, and intraventricular septum only. Left ventricular free wall data were log transformed and an unpaired Student t test was performed. B, Frozen sections (5 μm) of cardiac tissue were stained with wheat germ agglutinin and imaged for measurements of myocyte width. Consecutive sections of the same representative images were then stained for hematoxylin-and-eosin-stained sections. Scale bar, 50 μm for all images. Only myocytes in longitudinal axis cross-section were measured and widths measured from a total of 80–100 cells from two separate images per sample. Open bars [control (Ctrl)] and filled bars (Mat-Ob). Data are expressed as means ± sem . RNA from ventricular tissue of control and Mat-Ob males at 8 wk of age (n = 7 and 9, respectively, in which one offspring represents one litter) was isolated, purified, and cDNA obtained by reverse transcription. C, Gene expression of Nppb , Nppa , Myh7 , and Myh6 was quantified by real-time PCR using primers shown in Supplemental Table 1. All data were normalized to Gapdh as described in Materials and Methods . The ratio of Mhy7 to Myh6 was analyzed by a Mann-Whitney U test and presented as means ± interquartile ranges (n = 7 control and n = 9 Mat-Ob for all analyses). ***, P

    Journal: Endocrinology

    Article Title: The Programming of Cardiac Hypertrophy in the Offspring by Maternal Obesity Is Associated with Hyperinsulinemia, AKT, ERK, and mTOR Activation

    doi: 10.1210/en.2012-1508

    Figure Lengend Snippet: A, Combined left ventricular wall width (includes both the intraventricular septum and left ventricular free wall), left ventricular free wall only, and intraventricular septum only. Left ventricular free wall data were log transformed and an unpaired Student t test was performed. B, Frozen sections (5 μm) of cardiac tissue were stained with wheat germ agglutinin and imaged for measurements of myocyte width. Consecutive sections of the same representative images were then stained for hematoxylin-and-eosin-stained sections. Scale bar, 50 μm for all images. Only myocytes in longitudinal axis cross-section were measured and widths measured from a total of 80–100 cells from two separate images per sample. Open bars [control (Ctrl)] and filled bars (Mat-Ob). Data are expressed as means ± sem . RNA from ventricular tissue of control and Mat-Ob males at 8 wk of age (n = 7 and 9, respectively, in which one offspring represents one litter) was isolated, purified, and cDNA obtained by reverse transcription. C, Gene expression of Nppb , Nppa , Myh7 , and Myh6 was quantified by real-time PCR using primers shown in Supplemental Table 1. All data were normalized to Gapdh as described in Materials and Methods . The ratio of Mhy7 to Myh6 was analyzed by a Mann-Whitney U test and presented as means ± interquartile ranges (n = 7 control and n = 9 Mat-Ob for all analyses). ***, P

    Article Snippet: Quantitative real-time PCR Snap-frozen left ventricular tissue (20 mg) was placed into RNA-later solution (QIAGEN Ltd., Crawley, UK) on ice for 30 min. Tissue was then homogenized in lysis buffer and RNA extracted using the mirVana total RNA isolation kit (Ambion, Applied Biosystems, Life Technologies, Warrington, UK) as per the manufacturer's instructions.

    Techniques: Transformation Assay, Staining, Isolation, Purification, Expressing, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Chromatin marks at the IL12B locus. (A) A genome browser snapshot, including ChIP-seq tracks for α-H3K4me3, α-H3K4me1, α-H3K9me3, α-H3K27me3, α-C/EBPβ, α-H3K27ac, α-H3K36me3, IgG (unspecific polyclonal rabbit IgG pool) chromatin immunoprecipitations, and MIRA from a TAM sample freshly isolated from ovarian carcinoma ascites. Regions of interest are highlighted by rectangles. (B–D) ChIP-qPCR analyses of the indicated histone marks in an independent TAM sample at the indicated genomic locations amplified by specific primers are indicated. The error bars denote SDs from technical PCR replicates. (E–G) ChIP-qPCR analyses of the indicated histone marks in MDMs from three different pooled donor populations ( N = 3; N = 4 for IgG and α-H3K27me3 samples) differentiated in normal medium, in ascites, or in normal medium followed by ascites for 1 day. Cells were stimulated with or without LPS and IFNγ (+) or their respective solvents (−) 2.5 h prior to harvesting. Genomic regions were amplified by specific primers are indicated. Each dot denotes a biological replicate; for each replicate, MDMs from six donors were pooled after harvesting of the cells for each experiment. Median values are indicated by horizontal bars. Colors encode ascites samples from individual patients, and colors are consistent between panels within this figure, Figure 5 and Figure S9 in Supplementary Material. Statistical significances were calculated with paired t -tests. ** P

    Journal: Frontiers in Immunology

    Article Title: Chromatin Binding of c-REL and p65 Is Not Limiting for Macrophage IL12B Transcription During Immediate Suppression by Ovarian Carcinoma Ascites

    doi: 10.3389/fimmu.2018.01425

    Figure Lengend Snippet: Chromatin marks at the IL12B locus. (A) A genome browser snapshot, including ChIP-seq tracks for α-H3K4me3, α-H3K4me1, α-H3K9me3, α-H3K27me3, α-C/EBPβ, α-H3K27ac, α-H3K36me3, IgG (unspecific polyclonal rabbit IgG pool) chromatin immunoprecipitations, and MIRA from a TAM sample freshly isolated from ovarian carcinoma ascites. Regions of interest are highlighted by rectangles. (B–D) ChIP-qPCR analyses of the indicated histone marks in an independent TAM sample at the indicated genomic locations amplified by specific primers are indicated. The error bars denote SDs from technical PCR replicates. (E–G) ChIP-qPCR analyses of the indicated histone marks in MDMs from three different pooled donor populations ( N = 3; N = 4 for IgG and α-H3K27me3 samples) differentiated in normal medium, in ascites, or in normal medium followed by ascites for 1 day. Cells were stimulated with or without LPS and IFNγ (+) or their respective solvents (−) 2.5 h prior to harvesting. Genomic regions were amplified by specific primers are indicated. Each dot denotes a biological replicate; for each replicate, MDMs from six donors were pooled after harvesting of the cells for each experiment. Median values are indicated by horizontal bars. Colors encode ascites samples from individual patients, and colors are consistent between panels within this figure, Figure 5 and Figure S9 in Supplementary Material. Statistical significances were calculated with paired t -tests. ** P

    Article Snippet: Samples were washed once in buffer I (20 mM Tris pH 8.1; 150 mM NaCl; 1% (v/v) Triton X-100; 0.1% (w/v) SDS; 2 mM EDTA), once in buffer II (20 mM Tris pH 8.1; 500 mM NaCl; 1% (v/v) Triton X-100; 0.1% (w/v) SDS; 2 mM EDTA), twice in buffer III (10 mM Tris pH 8.1; 250 mM LiCl; 1% (v/v) NP40; 1% (w/v) sodium deoxycholate; 1 mM EDTA) on ice, and twice in Qiagen buffer EB (no. 19086) at room temperature.

    Techniques: Chromatin Immunoprecipitation, Isolation, Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction

    Schematic from PCR gene cloning to Agrobacterium expression clones using the Gateway ® cloning technology. Col E1 ori : origin of replication for E. coli . pVS1 ori: origin of replication for Agrobacteria . Kan r : kanamycin resistance gene. Cm r : chloramphenicol resistance gene. RB: T-DNA right border. LB: T-DNA left border. att L, att R, att B: recombination sites. ccdB: E. coli ccdB gene (note: the ccdB protein interferes with E. coli DNA gyrase and inhibits growth of most E. coli strains, e.g., DH5α™).

    Journal: Plant Methods

    Article Title: Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway® TOPO vector system

    doi: 10.1186/1746-4811-4-4

    Figure Lengend Snippet: Schematic from PCR gene cloning to Agrobacterium expression clones using the Gateway ® cloning technology. Col E1 ori : origin of replication for E. coli . pVS1 ori: origin of replication for Agrobacteria . Kan r : kanamycin resistance gene. Cm r : chloramphenicol resistance gene. RB: T-DNA right border. LB: T-DNA left border. att L, att R, att B: recombination sites. ccdB: E. coli ccdB gene (note: the ccdB protein interferes with E. coli DNA gyrase and inhibits growth of most E. coli strains, e.g., DH5α™).

    Article Snippet: High fidelity DNA polymerase (e.g., Pfu DNA polymerase) and appropriate 10× PCR buffer. dNTPs The PCR product and gel purification kit (e.g., QIAquick® from Qiagen Inc.).

    Techniques: Polymerase Chain Reaction, Clone Assay, Expressing

    Fingerprinting Xanthomonas isolates by REP-PCR and agarose gel electrophoresis. Lanes: M1, λ × Hin dIII (Life Technologies, Gaithersburg, Md.); M2 = kilobase DNA ladder (Stratagene, La Jolla, Calif.); 1, E. coli strain B; 2, P. putida 39169; 3, X. campestris pv. campestris 33913; 4, X. campestris pv. campestris X3; 5, X. campestris pv. campestris KX-1; 6, X. axonopodis pv. citrumelo 3048; 7, X. axonopodis pv. malvacearum N; 8, X. axonopodis pv. axonopodis 19132; 9, X. axonopodis pv. citri 62; 10, X. axonopodis pv. citri 3213; 11, X. axonopodis pv. citri 100; 12, X. axonopodis pv. citri 166; 13, X. axonopodis pv. citri 169; 14, X. oryzae pv. oryzae 43836; 15, X. oryzae pv. oryzae 43837; 16, X. oryzae pv. oryzicola 49072; 17, no-template control.

    Journal: Applied and Environmental Microbiology

    Article Title: Fingerprinting Closely Related Xanthomonas Pathovars with Random Nonamer Oligonucleotide Microarrays

    doi: 10.1128/AEM.68.12.6361-6370.2002

    Figure Lengend Snippet: Fingerprinting Xanthomonas isolates by REP-PCR and agarose gel electrophoresis. Lanes: M1, λ × Hin dIII (Life Technologies, Gaithersburg, Md.); M2 = kilobase DNA ladder (Stratagene, La Jolla, Calif.); 1, E. coli strain B; 2, P. putida 39169; 3, X. campestris pv. campestris 33913; 4, X. campestris pv. campestris X3; 5, X. campestris pv. campestris KX-1; 6, X. axonopodis pv. citrumelo 3048; 7, X. axonopodis pv. malvacearum N; 8, X. axonopodis pv. axonopodis 19132; 9, X. axonopodis pv. citri 62; 10, X. axonopodis pv. citri 3213; 11, X. axonopodis pv. citri 100; 12, X. axonopodis pv. citri 166; 13, X. axonopodis pv. citri 169; 14, X. oryzae pv. oryzae 43836; 15, X. oryzae pv. oryzae 43837; 16, X. oryzae pv. oryzicola 49072; 17, no-template control.

    Article Snippet: Final reaction conditions were (a minimum of) 150 ng of genomic DNA and 1× PCR buffer (Qiagen), 2.5 mM Mg2+ , 200 μM each dNTP, 1 U of Taq polymerase, and 0.6 μM each REP primer.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis