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polylinker sequence  (TaKaRa)
99
TaKaRa polylinker sequence
Polylinker Sequence, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polylinker+sequences/pm38232124-232-1-36?v=TaKaRa
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pvu ii restriction fragment containing polylinker sequences plasmid pgem5zf  (Promega)
90
Promega pvu ii restriction fragment containing polylinker sequences plasmid pgem5zf
Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid <t>pGEM5Zf</t> (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.
Pvu Ii Restriction Fragment Containing Polylinker Sequences Plasmid Pgem5zf, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polylinker+sequences/pmc06081617-274-66-68?v=Promega
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pvu ii restriction fragment containing polylinker sequences plasmid pgem5zf - by Bioz Stars, 2026-08
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polylinker sequence from pgl3-basic  (Promega)
90
Promega polylinker sequence from pgl3-basic
Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid <t>pGEM5Zf</t> (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.
Polylinker Sequence From Pgl3 Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polylinker+sequences/pmc02844622-422-11-12?v=Promega
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polylinker sequence from pgl3-basic - by Bioz Stars, 2026-08
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gateway® destination vector, pfastbacmam-2-rfa (a variant of the plasmid described in [ ], containing additional polylinker sequences)  (Thermo Fisher)
90
Thermo Fisher gateway® destination vector, pfastbacmam-2-rfa (a variant of the plasmid described in [ ], containing additional polylinker sequences)
Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid <t>pGEM5Zf</t> (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.
Gateway® Destination Vector, Pfastbacmam 2 Rfa (A Variant Of The Plasmid Described In [ ], Containing Additional Polylinker Sequences), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polylinker+sequences/pmc02669217-29-27-46?v=Thermo+Fisher
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gateway® destination vector, pfastbacmam-2-rfa (a variant of the plasmid described in [ ], containing additional polylinker sequences) - by Bioz Stars, 2026-08
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fire lab gfp vector polylinker sequence  (Addgene inc)
90
Addgene inc fire lab gfp vector polylinker sequence
Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid <t>pGEM5Zf</t> (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.
Fire Lab Gfp Vector Polylinker Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polylinker+sequences/pm18402933-59-30-36?v=Addgene+inc
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fire lab gfp vector polylinker sequence - by Bioz Stars, 2026-08
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transcrip- tion terminator and polylinker sequences  (TranScrip Partners)
90
TranScrip Partners transcrip- tion terminator and polylinker sequences
Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid <t>pGEM5Zf</t> (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.
Transcrip Tion Terminator And Polylinker Sequences, supplied by TranScrip Partners, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polylinker+sequences/10__1128_slash_jb__01279___06-203-19-15?v=TranScrip+Partners
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transcrip- tion terminator and polylinker sequences - by Bioz Stars, 2026-08
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polylinker sequence 5  (TaKaRa)
86
TaKaRa polylinker sequence 5
Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid <t>pGEM5Zf</t> (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.
Polylinker Sequence 5, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polylinker+sequences/pm12767060-43-16-37?v=TaKaRa
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polylinker sequence 5 - by Bioz Stars, 2026-08
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polylinker sequences  (Integrated DNA Technologies)
94
Integrated DNA Technologies polylinker sequences
Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid <t>pGEM5Zf</t> (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.
Polylinker Sequences, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polylinker+sequences/10__1128_slash_mcb__20__18__7037___7048__2000-79-21-9?v=Integrated+DNA+Technologies
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polylinker sequences - by Bioz Stars, 2026-08
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Image Search Results


Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid pGEM5Zf (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.

Journal: Gene Expression

Article Title: Role of Saccharomyces cerevisiae Rap1 protein in Ty1 and Ty1-mediated transcription

doi:

Figure Lengend Snippet: Factor binding to a Ty1 fragment I (approximately 50 bp) containing either a wild-type (I; lanes 1–4) or mutant (I*R; lane 5) RAP1 binding site and to a 22 bp oligonucleotide containing the Ty1 RAP1 binding site (lanes 8–11). Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820; lanes 1–5, 9) or rap1-1 (JF1165; lane 10) extracts. The rap1-1 strain was transformed with the RAP1 deletion plasmid pBG51 (see Materials and Methods), and extracts were prepared from cultures grown at room temperature and shifted to 37°C (lane 10). Reactions were performed in the absence of specific competitor (none) or with a 50-fold molar excess of fragment I (lane 2), oligonucleotide duplex containing the RAP1-binding site from the TEF2 gene (TEF2; lane 3), or a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid pGEM5Zf (Promega; lane 4). Positions of free probe and complex 1 (I-1 or RBSTy1-1) are indicated to the left of the autoradiogram. In lanes 6, 7, and 8, the extract was omitted. Lanes 1–7 and lanes 8–10 are taken from separate gels. Note also that a different radiolabeled probe was used in the two experiments.

Article Snippet: Reactions were performed in the absence of competitor (–; lanes 1, 5, and 8); or in the presence of a 50-fold molar excess of fragment D (lane 2); oligonucleotide duplex containing the RAP1 binding site from the TEF2 gene (TEF2; lanes 3, 9); oligonucleotide duplex containing the palindromic Mcm1 binding site (PAL; lane 4); a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid pGEM5Zf (Promega; lanes 7, 10); or fragment D* R containing a mutation in the RAP1 binding site (lane 6).

Techniques: Binding Assay, Mutagenesis, Transformation Assay, Plasmid Preparation

Factor binding to Ty1 fragment D containing either a wild-type (D) or a mutant (D*R) RAP1 binding site. Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820) or mcm1-1 (JF1096) strains. Reactions were performed in the absence of competitor (–; lanes 1, 5, and 8); or in the presence of a 50-fold molar excess of fragment D (lane 2); oligonucleotide duplex containing the RAP1 binding site from the TEF2 gene (TEF2; lanes 3, 9); oligonucleotide duplex containing the palindromic Mcm1 binding site (PAL; lane 4); a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid pGEM5Zf (Promega; lanes 7, 10); or fragment D*R containing a mutation in the RAP1 binding site (lane 6). In lanes 11 and 12, extract was omitted. Positions of the free probe, complex D-1, D-2, and D-3 are indicated on each side of the autoradiogram. To the right is a schematic of the proteins known to be present in each complex.

Journal: Gene Expression

Article Title: Role of Saccharomyces cerevisiae Rap1 protein in Ty1 and Ty1-mediated transcription

doi:

Figure Lengend Snippet: Factor binding to Ty1 fragment D containing either a wild-type (D) or a mutant (D*R) RAP1 binding site. Binding reaction mixtures contained 2–4 μg of protein from wild-type (JF820) or mcm1-1 (JF1096) strains. Reactions were performed in the absence of competitor (–; lanes 1, 5, and 8); or in the presence of a 50-fold molar excess of fragment D (lane 2); oligonucleotide duplex containing the RAP1 binding site from the TEF2 gene (TEF2; lanes 3, 9); oligonucleotide duplex containing the palindromic Mcm1 binding site (PAL; lane 4); a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid pGEM5Zf (Promega; lanes 7, 10); or fragment D*R containing a mutation in the RAP1 binding site (lane 6). In lanes 11 and 12, extract was omitted. Positions of the free probe, complex D-1, D-2, and D-3 are indicated on each side of the autoradiogram. To the right is a schematic of the proteins known to be present in each complex.

Article Snippet: Reactions were performed in the absence of competitor (–; lanes 1, 5, and 8); or in the presence of a 50-fold molar excess of fragment D (lane 2); oligonucleotide duplex containing the RAP1 binding site from the TEF2 gene (TEF2; lanes 3, 9); oligonucleotide duplex containing the palindromic Mcm1 binding site (PAL; lane 4); a 465 bp Pvu II restriction fragment containing polylinker (PL) sequences from plasmid pGEM5Zf (Promega; lanes 7, 10); or fragment D* R containing a mutation in the RAP1 binding site (lane 6).

Techniques: Binding Assay, Mutagenesis, Plasmid Preparation