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Polysciences inc polyethyleneimine
Schematic synthesis of brevinin 2R (BR-2R)-linked <t>polyethylenimine</t> (PEI) by EDC/NHS conjugation method
Polyethyleneimine, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 94/100, based on 403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyethyleneimine/product/Polysciences inc
Average 94 stars, based on 403 article reviews
Price from $9.99 to $1999.99
polyethyleneimine - by Bioz Stars, 2020-08
94/100 stars

Images

1) Product Images from "Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector "

Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

Journal: Iranian Journal of Basic Medical Sciences

doi: 10.22038/ijbms.2019.37125.8842

Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by EDC/NHS conjugation method
Figure Legend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by EDC/NHS conjugation method

Techniques Used: Conjugation Assay

Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by 6-bromohexanoic acid linker
Figure Legend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by 6-bromohexanoic acid linker

Techniques Used:

Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by SPDP conjugation method
Figure Legend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by SPDP conjugation method

Techniques Used: Conjugation Assay

2) Product Images from "Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9"

Article Title: Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9

Journal: Molecular Therapy. Methods & Clinical Development

doi: 10.1016/j.omtm.2018.10.015

Schematic Representation of rAAV9 Production and Purification HEK293EB cells were transfected with three plasmids ( cis AAV vector plasmid, trans plasmid, and helper plasmid) using polyethyleneimine (PEI, Polyethyleneimine Max) and maintained in DMEM without serum. After collecting the culture supernatant, it was ultrafiltrated by TFF with a hollow fiber using the KrosFlo Research Ili system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV9 was precipitated in 1/2-saturated AS solution (1/3→1/2 AS). The precipitated rAAV fraction was loaded onto a quaternary ammonium anion-exchange column. The pass-through fraction was finally purified by size-exclusion chromatography.
Figure Legend Snippet: Schematic Representation of rAAV9 Production and Purification HEK293EB cells were transfected with three plasmids ( cis AAV vector plasmid, trans plasmid, and helper plasmid) using polyethyleneimine (PEI, Polyethyleneimine Max) and maintained in DMEM without serum. After collecting the culture supernatant, it was ultrafiltrated by TFF with a hollow fiber using the KrosFlo Research Ili system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV9 was precipitated in 1/2-saturated AS solution (1/3→1/2 AS). The precipitated rAAV fraction was loaded onto a quaternary ammonium anion-exchange column. The pass-through fraction was finally purified by size-exclusion chromatography.

Techniques Used: Purification, Transfection, Plasmid Preparation, Size-exclusion Chromatography

3) Product Images from "Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1)"

Article Title: Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1)

Journal: Molecular Therapy. Methods & Clinical Development

doi: 10.1038/mtm.2015.58

Schematic representation of the recombinant adeno-associated virus serotype 1 (rAAV1) production and purification procedure. Three plasmids (the cis AAV vector plasmid, trans-plasmid, and helper plasmid) were transfected to HEK293 cells with PEI (Polyethyleneimine Max) in serum-free medium. After harvesting the culture supernatant, it was ultrafiltrated with TFF using the hollow fiber using the KrosFlo Research IIi system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV1 was finally precipitated in 1/2-saturated AS solution (1/3→1/2 AS). Then, it was dialyzed against a low-salt buffer (containing 5 mmol/l NaCl) and absorbed to ion-exchange membranes. Finally, rAAV1 eluted from the anion-exchange column was purified by gel-filtration chromatography. AS, ammonium sulfate; FCS, fetal calf serum; PEI, polyethyleneimine.
Figure Legend Snippet: Schematic representation of the recombinant adeno-associated virus serotype 1 (rAAV1) production and purification procedure. Three plasmids (the cis AAV vector plasmid, trans-plasmid, and helper plasmid) were transfected to HEK293 cells with PEI (Polyethyleneimine Max) in serum-free medium. After harvesting the culture supernatant, it was ultrafiltrated with TFF using the hollow fiber using the KrosFlo Research IIi system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV1 was finally precipitated in 1/2-saturated AS solution (1/3→1/2 AS). Then, it was dialyzed against a low-salt buffer (containing 5 mmol/l NaCl) and absorbed to ion-exchange membranes. Finally, rAAV1 eluted from the anion-exchange column was purified by gel-filtration chromatography. AS, ammonium sulfate; FCS, fetal calf serum; PEI, polyethyleneimine.

Techniques Used: Recombinant, Purification, Plasmid Preparation, Transfection, Filtration, Chromatography

4) Product Images from "Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9"

Article Title: Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9

Journal: Molecular Therapy. Methods & Clinical Development

doi: 10.1016/j.omtm.2018.10.015

Schematic Representation of rAAV9 Production and Purification HEK293EB cells were transfected with three plasmids ( cis AAV vector plasmid, trans plasmid, and helper plasmid) using polyethyleneimine (PEI, Polyethyleneimine Max) and maintained in DMEM without serum. After collecting the culture supernatant, it was ultrafiltrated by TFF with a hollow fiber using the KrosFlo Research Ili system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV9 was precipitated in 1/2-saturated AS solution (1/3→1/2 AS). The precipitated rAAV fraction was loaded onto a quaternary ammonium anion-exchange column. The pass-through fraction was finally purified by size-exclusion chromatography.
Figure Legend Snippet: Schematic Representation of rAAV9 Production and Purification HEK293EB cells were transfected with three plasmids ( cis AAV vector plasmid, trans plasmid, and helper plasmid) using polyethyleneimine (PEI, Polyethyleneimine Max) and maintained in DMEM without serum. After collecting the culture supernatant, it was ultrafiltrated by TFF with a hollow fiber using the KrosFlo Research Ili system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV9 was precipitated in 1/2-saturated AS solution (1/3→1/2 AS). The precipitated rAAV fraction was loaded onto a quaternary ammonium anion-exchange column. The pass-through fraction was finally purified by size-exclusion chromatography.

Techniques Used: Purification, Transfection, Plasmid Preparation, Size-exclusion Chromatography

5) Product Images from "Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1)"

Article Title: Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1)

Journal: Molecular Therapy. Methods & Clinical Development

doi: 10.1038/mtm.2015.58

Schematic representation of the recombinant adeno-associated virus serotype 1 (rAAV1) production and purification procedure. Three plasmids (the cis AAV vector plasmid, trans-plasmid, and helper plasmid) were transfected to HEK293 cells with PEI (Polyethyleneimine Max) in serum-free medium. After harvesting the culture supernatant, it was ultrafiltrated with TFF using the hollow fiber using the KrosFlo Research IIi system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV1 was finally precipitated in 1/2-saturated AS solution (1/3→1/2 AS). Then, it was dialyzed against a low-salt buffer (containing 5 mmol/l NaCl) and absorbed to ion-exchange membranes. Finally, rAAV1 eluted from the anion-exchange column was purified by gel-filtration chromatography. AS, ammonium sulfate; FCS, fetal calf serum; PEI, polyethyleneimine.
Figure Legend Snippet: Schematic representation of the recombinant adeno-associated virus serotype 1 (rAAV1) production and purification procedure. Three plasmids (the cis AAV vector plasmid, trans-plasmid, and helper plasmid) were transfected to HEK293 cells with PEI (Polyethyleneimine Max) in serum-free medium. After harvesting the culture supernatant, it was ultrafiltrated with TFF using the hollow fiber using the KrosFlo Research IIi system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV1 was finally precipitated in 1/2-saturated AS solution (1/3→1/2 AS). Then, it was dialyzed against a low-salt buffer (containing 5 mmol/l NaCl) and absorbed to ion-exchange membranes. Finally, rAAV1 eluted from the anion-exchange column was purified by gel-filtration chromatography. AS, ammonium sulfate; FCS, fetal calf serum; PEI, polyethyleneimine.

Techniques Used: Recombinant, Purification, Plasmid Preparation, Transfection, Filtration, Chromatography

Related Articles

Transfection:

Article Title: Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1)
Article Snippet: .. Production of rAAV1 After the cells were grown to more than 90% confluence, they were transfected with the cis AAV vector plasmid (with AAV-type 2 inverted terminal repeats), the trans-plasmid (with the AAV-type 2 Rep gene and the type 1 Cap gene; p5E18RXC1; a gift from James M. Wilson, University of Pennsylvania), and an adenovirus helper plasmid (with an essential region from the adenovirus genome; pHelper, Stratagene, La Jolla, CA) at a ratio of 1:1:2 , in serum-free medium and GlutaMAX-І (1×; Gibco Life Technologies, Grand Island, NY) using polyethyleneimine (Polyethyleneimine Max, Polysciences) at a DNA:polyethyleneimine ratio of 1:2 for 5 days. .. The cis AAV vector plasmid expressing the EGFP gene under the control of the CBA promoter, pdsAAV-CB-EGFP, was used in this study (provided by Dr. Arun Srivastava, University of Florida).

Article Title: Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9
Article Snippet: .. The cis , trans , and adenovirus helper plasmids were transfected at a ratio of 1:1:2 , (44.8 μg of cis plasmid, 44.8 μg of trans plasmid, and 90.1 μg of pHelper per square dish) to cells maintained in DMEM without serum supplemented with GlutaMAX-І (Gibco, Life Technologies) and using polyethyleneimine (PEI Max, Polysciences, Warrington, PA) at a DNA:PEI ratio of 1:2. .. The culture supernatant was collected 120 h after transfection, filtrated through a 0.45-μm filter (Thermo Fisher Scientific, Waltham, MA), and ultrafiltrated by TFF using a hollow fiber cartridge (UFP-750-E-3MA; 750,000 nominal molecular weight cutoff; GE Healthcare, Westborough, MA) using the KrosFlo Research Ili TFF system (Spectrum Laboratories, Rancho Dominguez, CA).

Article Title: A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors
Article Snippet: .. For suspension cells, transfection was carried out with polyethylenimine ‘Max’ high-potency linear PEI (Polysciences) as described . ..

Article Title: Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9
Article Snippet: .. The cis , trans , and adenovirus helper plasmids were transfected at a ratio of 1:1:2 , (44.8 μg of cis plasmid, 44.8 μg of trans plasmid, and 90.1 μg of pHelper per square dish) to cells maintained in DMEM without serum supplemented with GlutaMAX-І (Gibco, Life Technologies) and using polyethyleneimine (PEI Max, Polysciences, Warrington, PA) at a DNA:PEI ratio of 1:2. .. The culture supernatant was collected 120 h after transfection, filtrated through a 0.45-μm filter (Thermo Fisher Scientific, Waltham, MA), and ultrafiltrated by TFF using a hollow fiber cartridge (UFP-750-E-3MA; 750,000 nominal molecular weight cutoff; GE Healthcare, Westborough, MA) using the KrosFlo Research Ili TFF system (Spectrum Laboratories, Rancho Dominguez, CA).

other:

Article Title: Efficient suppression of secretory clusterin levels by polymer-siRNA nanocomplexes enhances ionizing radiation lethality in human MCF-7 breast cancer cells in vitro *
Article Snippet: Materials Branched polyethylenimine (PEI, MW 10 kDa) was purchased from Polysciences (Warrington, PA, USA).

Plasmid Preparation:

Article Title: GTP binding controls complex formation by the human ROCO protein MASL1
Article Snippet: .. To transfect cells 20 μg of plasmid DNA was dissolved in 500 μL DMEM before 40 μL polyethylenimine (PEI, Polysciences, Warrington, PA, USA) was added and the solution was mixed and left to incubate for a minimum of 15 min at room temperature. ..

Article Title: Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1)
Article Snippet: .. Production of rAAV1 After the cells were grown to more than 90% confluence, they were transfected with the cis AAV vector plasmid (with AAV-type 2 inverted terminal repeats), the trans-plasmid (with the AAV-type 2 Rep gene and the type 1 Cap gene; p5E18RXC1; a gift from James M. Wilson, University of Pennsylvania), and an adenovirus helper plasmid (with an essential region from the adenovirus genome; pHelper, Stratagene, La Jolla, CA) at a ratio of 1:1:2 , in serum-free medium and GlutaMAX-І (1×; Gibco Life Technologies, Grand Island, NY) using polyethyleneimine (Polyethyleneimine Max, Polysciences) at a DNA:polyethyleneimine ratio of 1:2 for 5 days. .. The cis AAV vector plasmid expressing the EGFP gene under the control of the CBA promoter, pdsAAV-CB-EGFP, was used in this study (provided by Dr. Arun Srivastava, University of Florida).

Article Title: Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9
Article Snippet: .. The cis , trans , and adenovirus helper plasmids were transfected at a ratio of 1:1:2 , (44.8 μg of cis plasmid, 44.8 μg of trans plasmid, and 90.1 μg of pHelper per square dish) to cells maintained in DMEM without serum supplemented with GlutaMAX-І (Gibco, Life Technologies) and using polyethyleneimine (PEI Max, Polysciences, Warrington, PA) at a DNA:PEI ratio of 1:2. .. The culture supernatant was collected 120 h after transfection, filtrated through a 0.45-μm filter (Thermo Fisher Scientific, Waltham, MA), and ultrafiltrated by TFF using a hollow fiber cartridge (UFP-750-E-3MA; 750,000 nominal molecular weight cutoff; GE Healthcare, Westborough, MA) using the KrosFlo Research Ili TFF system (Spectrum Laboratories, Rancho Dominguez, CA).

Article Title: Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9
Article Snippet: .. The cis , trans , and adenovirus helper plasmids were transfected at a ratio of 1:1:2 , (44.8 μg of cis plasmid, 44.8 μg of trans plasmid, and 90.1 μg of pHelper per square dish) to cells maintained in DMEM without serum supplemented with GlutaMAX-І (Gibco, Life Technologies) and using polyethyleneimine (PEI Max, Polysciences, Warrington, PA) at a DNA:PEI ratio of 1:2. .. The culture supernatant was collected 120 h after transfection, filtrated through a 0.45-μm filter (Thermo Fisher Scientific, Waltham, MA), and ultrafiltrated by TFF using a hollow fiber cartridge (UFP-750-E-3MA; 750,000 nominal molecular weight cutoff; GE Healthcare, Westborough, MA) using the KrosFlo Research Ili TFF system (Spectrum Laboratories, Rancho Dominguez, CA).

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    Polysciences inc polyethyleneimine
    Schematic synthesis of brevinin 2R (BR-2R)-linked <t>polyethylenimine</t> (PEI) by EDC/NHS conjugation method
    Polyethyleneimine, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 94/100, based on 821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine/product/Polysciences inc
    Average 94 stars, based on 821 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    88
    Polysciences inc polyethylenimine 25
    In-vitro evaluation of polytope expression using COS-7 cells. Recombinant pEFGP-PT was transiently transfected into COS-7 cells by means of linear <t>Polyethylenimine</t> 25 KDa. A1 represents COS-7 cells without any transfection, A2 represents COS-7 cells transfected with pEGFP-N3 as positive control and A3 represents COS-7 cells transfected with pEGFP-PT. Panel B represents the corresponding microscopic feature of each condition, B1 represents positive control and B2 represents COS-7 cells transfected with pEGFP-PT after 24 hours.
    Polyethylenimine 25, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethylenimine 25/product/Polysciences inc
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    polyethylenimine 25 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by EDC/NHS conjugation method

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by EDC/NHS conjugation method

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques: Conjugation Assay

    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by 6-bromohexanoic acid linker

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by 6-bromohexanoic acid linker

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques:

    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by SPDP conjugation method

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by SPDP conjugation method

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques: Conjugation Assay

    PEI-g-PEG copolymers complex with siRNA and exclude EtBr. The fluorescence intensity was normalized to the siRNA–EtBr complex prior to addition of copolymers. For each copolymer, 2k refers to the molecular weight of the grafting PEG chain and 5, 10, or 15 refer to the number of PEG grafts per PEI molecule. The inset shows the hydrodynamic diameters of the polymer–siRNA nanocomplexes as measured by dynamic light scattering. Abbreviations: EtBr, ethidium bromide; PEG, polyethylene glycol; PEI, polyethylenimine; siRNA, small interfering RNA.

    Journal: International Journal of Nanomedicine

    Article Title: Efficient suppression of secretory clusterin levels by polymer-siRNA nanocomplexes enhances ionizing radiation lethality in human MCF-7 breast cancer cells in vitro *

    doi:

    Figure Lengend Snippet: PEI-g-PEG copolymers complex with siRNA and exclude EtBr. The fluorescence intensity was normalized to the siRNA–EtBr complex prior to addition of copolymers. For each copolymer, 2k refers to the molecular weight of the grafting PEG chain and 5, 10, or 15 refer to the number of PEG grafts per PEI molecule. The inset shows the hydrodynamic diameters of the polymer–siRNA nanocomplexes as measured by dynamic light scattering. Abbreviations: EtBr, ethidium bromide; PEG, polyethylene glycol; PEI, polyethylenimine; siRNA, small interfering RNA.

    Article Snippet: Materials Branched polyethylenimine (PEI, MW 10 kDa) was purchased from Polysciences (Warrington, PA, USA).

    Techniques: Fluorescence, Molecular Weight, Small Interfering RNA

    Schematic Representation of rAAV9 Production and Purification HEK293EB cells were transfected with three plasmids ( cis AAV vector plasmid, trans plasmid, and helper plasmid) using polyethyleneimine (PEI, Polyethyleneimine Max) and maintained in DMEM without serum. After collecting the culture supernatant, it was ultrafiltrated by TFF with a hollow fiber using the KrosFlo Research Ili system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV9 was precipitated in 1/2-saturated AS solution (1/3→1/2 AS). The precipitated rAAV fraction was loaded onto a quaternary ammonium anion-exchange column. The pass-through fraction was finally purified by size-exclusion chromatography.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9

    doi: 10.1016/j.omtm.2018.10.015

    Figure Lengend Snippet: Schematic Representation of rAAV9 Production and Purification HEK293EB cells were transfected with three plasmids ( cis AAV vector plasmid, trans plasmid, and helper plasmid) using polyethyleneimine (PEI, Polyethyleneimine Max) and maintained in DMEM without serum. After collecting the culture supernatant, it was ultrafiltrated by TFF with a hollow fiber using the KrosFlo Research Ili system. After reducing the amount of protein debris by 1/3-saturated AS precipitation, rAAV9 was precipitated in 1/2-saturated AS solution (1/3→1/2 AS). The precipitated rAAV fraction was loaded onto a quaternary ammonium anion-exchange column. The pass-through fraction was finally purified by size-exclusion chromatography.

    Article Snippet: The cis , trans , and adenovirus helper plasmids were transfected at a ratio of 1:1:2 , (44.8 μg of cis plasmid, 44.8 μg of trans plasmid, and 90.1 μg of pHelper per square dish) to cells maintained in DMEM without serum supplemented with GlutaMAX-І (Gibco, Life Technologies) and using polyethyleneimine (PEI Max, Polysciences, Warrington, PA) at a DNA:PEI ratio of 1:2.

    Techniques: Purification, Transfection, Plasmid Preparation, Size-exclusion Chromatography

    In-vitro evaluation of polytope expression using COS-7 cells. Recombinant pEFGP-PT was transiently transfected into COS-7 cells by means of linear Polyethylenimine 25 KDa. A1 represents COS-7 cells without any transfection, A2 represents COS-7 cells transfected with pEGFP-N3 as positive control and A3 represents COS-7 cells transfected with pEGFP-PT. Panel B represents the corresponding microscopic feature of each condition, B1 represents positive control and B2 represents COS-7 cells transfected with pEGFP-PT after 24 hours.

    Journal: PLoS ONE

    Article Title: Immunogenicity Evaluation of a Rationally Designed Polytope Construct Encoding HLA-A*0201 Restricted Epitopes Derived from Leishmania major Related Proteins in HLA-A2/DR1 Transgenic Mice: Steps toward Polytope Vaccine

    doi: 10.1371/journal.pone.0108848

    Figure Lengend Snippet: In-vitro evaluation of polytope expression using COS-7 cells. Recombinant pEFGP-PT was transiently transfected into COS-7 cells by means of linear Polyethylenimine 25 KDa. A1 represents COS-7 cells without any transfection, A2 represents COS-7 cells transfected with pEGFP-N3 as positive control and A3 represents COS-7 cells transfected with pEGFP-PT. Panel B represents the corresponding microscopic feature of each condition, B1 represents positive control and B2 represents COS-7 cells transfected with pEGFP-PT after 24 hours.

    Article Snippet: In-vitro evaluation of polytope expression using COS-7 cells Recombinant pEFGP-PT was purified (Qiagen midi-plasmid purification kit, Germany) and transiently transfected into COS-7 cells by means of linear Polyethylenimine 25 KDa (LINPEI.25-Polysciences, USA), as previously described .

    Techniques: In Vitro, Expressing, Recombinant, Transfection, Positive Control