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Polyplus Transfection polyethyleneimine
Polyethyleneimine, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Transfection:

Article Title: Coordination of Myeloid Differentiation with Reduced Cell Cycle Progression by PU.1 Induction of MicroRNAs Targeting Cell Cycle Regulators and Lipid Anabolism
Article Snippet: .. To generate retroviral particles, platinum E (Plat-E) retroviral packaging cells were transfected with plasmids using polyethylenimine (PEIpro; PolyPlus, Illkirch, France). ..

Article Title: Murine Insulin Growth Factor-like (IGFL) and Human IGFL1 Proteins Are Induced in Inflammatory Skin Conditions and Bind to a Novel Tumor Necrosis Factor Receptor Family Member, IGFLR1 *
Article Snippet: .. Transient transfection of DNA into CHO cells was accomplished using DMRIE-C transfection reagent (Invitrogen catalog number 10459-014) or the cationic polymer, polyethyleneimine (Polyplus catalog 101-40N), as previously described ( , ). .. FLAG-tagged, His8 -tagged, and IgG1 -Fc fusion proteins were purified from the transient conditioned media as follows.

Article Title: Complement Factor H-Related 5-Hybrid Proteins Anchor Properdin and Activate Complement at Self-Surfaces
Article Snippet: .. Recombinant CFHR5 and CFHR5-associated mutants, and the N-terminal fragments, were expressed in HEK293 cells by transient transfection with polyethylenimine (jetPEI, Polyplus). ..

Article Title: SPLICELECT™: an adaptable cell surface display technology based on alternative splicing allowing the qualitative and quantitative prediction of secreted product at a single-cell level
Article Snippet: .. Transfections Transfections were performed using polyethyleneimine (JetPEI®, Polyplus-transfection) in tubespin. .. Cells were seeded at a density of 2 × 106 cells/mL in 5 mL of transfection medium (e.g., OptiMEM (#31985-047, Invitrogen)).

Recombinant:

Article Title: Complement Factor H-Related 5-Hybrid Proteins Anchor Properdin and Activate Complement at Self-Surfaces
Article Snippet: .. Recombinant CFHR5 and CFHR5-associated mutants, and the N-terminal fragments, were expressed in HEK293 cells by transient transfection with polyethylenimine (jetPEI, Polyplus). ..

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  • 90
    Polyplus Transfection polyethylenimine nps
    Identification of a target RNA-NP. (A) An immortalized murine bone marrow-derived DC line (DC2.4) was transfected with GFP RNA comparing the cationic liposome DOTAP to JETPEI and JETPEI-mannose, and cells were screened one day later for assessment of GFP expression via flow cytometry. (B) C57Bl/6 mice, were given a low precursor frequency of OT-Is, and vaccinated intravenously with OVA RNA or control RNA (luciferase) encapsulated in either DOTAP, or linear <t>polyethylenimine</t> <t>NPs</t> with DC targeting mannose receptors (JETPEI-Mannose); OVA-specific T cell immunity was assessed from spleens of vaccinated mice 1 week later by flow cytometry. (C) Different ratios of GFP RNA to NP DOTAP were encapsulated and assessed for %transfection efficiency of DC2.4s in vitro . Cells were grown in vitro and harvested 1 d after addition of RNA-NPs to culture media. (D) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on percent GFP transfection efficiency (left) and GFP-MFI (right) after 24 h. (E) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on percent GFP transfection efficiency (left) and GFP-MFI (right) after 72 h. (F) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on MHCI MFI 24 h after transfection of DC2.4s with GFP RNA. (* p
    Polyethylenimine Nps, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    88
    Polyplus Transfection linear polyethylenimine pei
    Phenotypes 24 h after Dbait injection into the extracellular space of cell stage 1 K zebrafish embryos. ( a – c ) Lateral views anterior to th e left of zebrafish embryos, 24 h after <t>Dbait-cy3+polyethylenimine</t> <t>(PEI)</t> injection (2–5 nl) at the animal pole of cell stage 1 K zebrafish embryos: top panel, bright field view; bottom panel, 2 × magnification of the head region with epifluorescence overlay showing in red Dbait-cy3, scale bar 250 μm. ( a ) Type 1 phenotype indistinguishable from non-injected (not shown). ( b ) Type 2 mild phenotype with extensive cell death in the head region. ( c ) Type 3 strong teratogenesis and widespread cell death. ( d ) Histogram showing the percentage of the three phenotypic classes depending on the adjuvant. More than 100 embryos were analyzed for each condition. Sup: Superfect; 25, 22, 11 K PEI of the corresponding size; Lu, Lutrol; CQ, chloroquine; ‘−', without adjuvant. ( e ) Histogram showing the percentage of the animals with type 1 phenotype after injections of nanoparticules formed with 8H, 64ss or Dbait32H and Superfect or PEI11K. The Dbait32H, and the 8H and 64ss inactive nanoparticules, were used at equivalent adjuvant concentrations.
    Linear Polyethylenimine Pei, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/linear polyethylenimine pei/product/Polyplus Transfection
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    89
    Polyplus Transfection pei plasmid dna complex polyethyleneimine pei
    Non viral-mediated expression of transgenes in rat salivary glands . (A) Diagram of the transfection procedures in anesthetized rats. A thin polyethylene cannula is inserted into the Wharton's duct, as described in the Methods sections. The plasmid <t>DNA</t> is gently injected and diffuses into the large ducts (granular convoluted tubules or GCT, and the striated ducts or SD), then into the intercalated ducts (IC), and finally into the acinar canaliculi. Plasmid DNA accesses the epithelium through the apical plasma membrane (APM, arrows). (B) In vivo transfection with plasmid DNA -12 μg of Plasmid DNA encoding for pVenus were injected alone (CTRL, left panel), after SC injection of isoproterenol (ISO, center panel), or mixed with <t>PEI</t> as described in the Methods (PEI, right panel). After 16 h the submandibular SGs were excised and immediately imaged by two-photon microscopy using a 10X objective (excitation 930 nm) to reveal the cells expressing pVenus (arrows). Scale bar, 1 mm. (C) Effect of PEI on regulated exocytosis. Mice expressing cytoplasmic GFP were treated as described in B and the SGs imaged by confocal microscopy (excitation 488 nm) to estimate exocytosis of the secretory granules, as previously reported (Milberg et al., 2013 ). In the inset the apical plasma membrane (arrows) and the secretory granules (arrowheads) are highlighted. Scale bar, 20 μm. (D–E) PEI-mediated transfection in SGs drives expression into acinar cells. (D) Excised glands transfected with pVenus/PEI were imaged by two-photon microscopy using a 60x water immersion objective (D) or processed for immunofluorescence and imaged by confocal microscopy (E) . (D) Two Z-stacks from the same area of the sample were acquired twice by using two different excitation wavelengths, respectively: 740 nm to reveal the epithelium of the gland (cyan) and 930 nm to reveal pVenus (green) and collagen fibers (red). The Z-stacks were combined and shown as xz (left) or xy (center) projection. The cell expressing pVenus is part of an acinus (inset, right). Scale bar, 20 μm. (E) The SGs were labeled for phallodin (red) and the nuclear staining Hoechst (blue). The cell expressing pVenus (green) is part of an acinus (white broken line) as identified by the actin-labeled acinar canaliculi (arrowhead). Large ducts (yellow broken line), as identified by the characteristic actin pattern (asterisk) did not express any pVenus. Scale bar, 10 μm. (F) Diagram summarizing the pattern of expression of plasmid DNA in SGs. pVenus was expressed in cells of the IC under control conditions (left) and in acinar cells either upon stimulation with ISO (center) or administration with PEI (right).
    Pei Plasmid Dna Complex Polyethyleneimine Pei, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pei plasmid dna complex polyethyleneimine pei/product/Polyplus Transfection
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    92
    Polyplus Transfection polyethyleneimine
    Non viral-mediated expression of transgenes in rat salivary glands . (A) Diagram of the transfection procedures in anesthetized rats. A thin polyethylene cannula is inserted into the Wharton's duct, as described in the Methods sections. The plasmid <t>DNA</t> is gently injected and diffuses into the large ducts (granular convoluted tubules or GCT, and the striated ducts or SD), then into the intercalated ducts (IC), and finally into the acinar canaliculi. Plasmid DNA accesses the epithelium through the apical plasma membrane (APM, arrows). (B) In vivo transfection with plasmid DNA -12 μg of Plasmid DNA encoding for pVenus were injected alone (CTRL, left panel), after SC injection of isoproterenol (ISO, center panel), or mixed with <t>PEI</t> as described in the Methods (PEI, right panel). After 16 h the submandibular SGs were excised and immediately imaged by two-photon microscopy using a 10X objective (excitation 930 nm) to reveal the cells expressing pVenus (arrows). Scale bar, 1 mm. (C) Effect of PEI on regulated exocytosis. Mice expressing cytoplasmic GFP were treated as described in B and the SGs imaged by confocal microscopy (excitation 488 nm) to estimate exocytosis of the secretory granules, as previously reported (Milberg et al., 2013 ). In the inset the apical plasma membrane (arrows) and the secretory granules (arrowheads) are highlighted. Scale bar, 20 μm. (D–E) PEI-mediated transfection in SGs drives expression into acinar cells. (D) Excised glands transfected with pVenus/PEI were imaged by two-photon microscopy using a 60x water immersion objective (D) or processed for immunofluorescence and imaged by confocal microscopy (E) . (D) Two Z-stacks from the same area of the sample were acquired twice by using two different excitation wavelengths, respectively: 740 nm to reveal the epithelium of the gland (cyan) and 930 nm to reveal pVenus (green) and collagen fibers (red). The Z-stacks were combined and shown as xz (left) or xy (center) projection. The cell expressing pVenus is part of an acinus (inset, right). Scale bar, 20 μm. (E) The SGs were labeled for phallodin (red) and the nuclear staining Hoechst (blue). The cell expressing pVenus (green) is part of an acinus (white broken line) as identified by the actin-labeled acinar canaliculi (arrowhead). Large ducts (yellow broken line), as identified by the characteristic actin pattern (asterisk) did not express any pVenus. Scale bar, 10 μm. (F) Diagram summarizing the pattern of expression of plasmid DNA in SGs. pVenus was expressed in cells of the IC under control conditions (left) and in acinar cells either upon stimulation with ISO (center) or administration with PEI (right).
    Polyethyleneimine, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    Identification of a target RNA-NP. (A) An immortalized murine bone marrow-derived DC line (DC2.4) was transfected with GFP RNA comparing the cationic liposome DOTAP to JETPEI and JETPEI-mannose, and cells were screened one day later for assessment of GFP expression via flow cytometry. (B) C57Bl/6 mice, were given a low precursor frequency of OT-Is, and vaccinated intravenously with OVA RNA or control RNA (luciferase) encapsulated in either DOTAP, or linear polyethylenimine NPs with DC targeting mannose receptors (JETPEI-Mannose); OVA-specific T cell immunity was assessed from spleens of vaccinated mice 1 week later by flow cytometry. (C) Different ratios of GFP RNA to NP DOTAP were encapsulated and assessed for %transfection efficiency of DC2.4s in vitro . Cells were grown in vitro and harvested 1 d after addition of RNA-NPs to culture media. (D) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on percent GFP transfection efficiency (left) and GFP-MFI (right) after 24 h. (E) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on percent GFP transfection efficiency (left) and GFP-MFI (right) after 72 h. (F) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on MHCI MFI 24 h after transfection of DC2.4s with GFP RNA. (* p

    Journal: Oncoimmunology

    Article Title: Systemic activation of antigen-presenting cells via RNA-loaded nanoparticles

    doi: 10.1080/2162402X.2016.1256527

    Figure Lengend Snippet: Identification of a target RNA-NP. (A) An immortalized murine bone marrow-derived DC line (DC2.4) was transfected with GFP RNA comparing the cationic liposome DOTAP to JETPEI and JETPEI-mannose, and cells were screened one day later for assessment of GFP expression via flow cytometry. (B) C57Bl/6 mice, were given a low precursor frequency of OT-Is, and vaccinated intravenously with OVA RNA or control RNA (luciferase) encapsulated in either DOTAP, or linear polyethylenimine NPs with DC targeting mannose receptors (JETPEI-Mannose); OVA-specific T cell immunity was assessed from spleens of vaccinated mice 1 week later by flow cytometry. (C) Different ratios of GFP RNA to NP DOTAP were encapsulated and assessed for %transfection efficiency of DC2.4s in vitro . Cells were grown in vitro and harvested 1 d after addition of RNA-NPs to culture media. (D) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on percent GFP transfection efficiency (left) and GFP-MFI (right) after 24 h. (E) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on percent GFP transfection efficiency (left) and GFP-MFI (right) after 72 h. (F) Cationic lipid preparations of DOTAP and DOTAP-DOPE were compared with RNA-iMAX lipofectamine based on MHCI MFI 24 h after transfection of DC2.4s with GFP RNA. (* p

    Article Snippet: Polyethylenimine NPs (JETPE, and JETPEI mannose) were obtained from Polyplus transfection and prepared using manufacturer's instructions.

    Techniques: Derivative Assay, Transfection, Expressing, Flow Cytometry, Cytometry, Mouse Assay, Luciferase, In Vitro

    Phenotypes 24 h after Dbait injection into the extracellular space of cell stage 1 K zebrafish embryos. ( a – c ) Lateral views anterior to th e left of zebrafish embryos, 24 h after Dbait-cy3+polyethylenimine (PEI) injection (2–5 nl) at the animal pole of cell stage 1 K zebrafish embryos: top panel, bright field view; bottom panel, 2 × magnification of the head region with epifluorescence overlay showing in red Dbait-cy3, scale bar 250 μm. ( a ) Type 1 phenotype indistinguishable from non-injected (not shown). ( b ) Type 2 mild phenotype with extensive cell death in the head region. ( c ) Type 3 strong teratogenesis and widespread cell death. ( d ) Histogram showing the percentage of the three phenotypic classes depending on the adjuvant. More than 100 embryos were analyzed for each condition. Sup: Superfect; 25, 22, 11 K PEI of the corresponding size; Lu, Lutrol; CQ, chloroquine; ‘−', without adjuvant. ( e ) Histogram showing the percentage of the animals with type 1 phenotype after injections of nanoparticules formed with 8H, 64ss or Dbait32H and Superfect or PEI11K. The Dbait32H, and the 8H and 64ss inactive nanoparticules, were used at equivalent adjuvant concentrations.

    Journal: Cancer Gene Therapy

    Article Title: Comparison of distribution and activity of nanoparticles with short interfering DNA (Dbait) in various living systems

    doi: 10.1038/cgt.2011.39

    Figure Lengend Snippet: Phenotypes 24 h after Dbait injection into the extracellular space of cell stage 1 K zebrafish embryos. ( a – c ) Lateral views anterior to th e left of zebrafish embryos, 24 h after Dbait-cy3+polyethylenimine (PEI) injection (2–5 nl) at the animal pole of cell stage 1 K zebrafish embryos: top panel, bright field view; bottom panel, 2 × magnification of the head region with epifluorescence overlay showing in red Dbait-cy3, scale bar 250 μm. ( a ) Type 1 phenotype indistinguishable from non-injected (not shown). ( b ) Type 2 mild phenotype with extensive cell death in the head region. ( c ) Type 3 strong teratogenesis and widespread cell death. ( d ) Histogram showing the percentage of the three phenotypic classes depending on the adjuvant. More than 100 embryos were analyzed for each condition. Sup: Superfect; 25, 22, 11 K PEI of the corresponding size; Lu, Lutrol; CQ, chloroquine; ‘−', without adjuvant. ( e ) Histogram showing the percentage of the animals with type 1 phenotype after injections of nanoparticules formed with 8H, 64ss or Dbait32H and Superfect or PEI11K. The Dbait32H, and the 8H and 64ss inactive nanoparticules, were used at equivalent adjuvant concentrations.

    Article Snippet: Linear polyethylenimine (PEI) (11 and 22 kDa) were obtained from Polyplus-Transfection (Illkirch, France) and provided as a ready-to-use solution of 300 m nitrogen concentration.

    Techniques: Injection

    Diffusion and activity in tumors. Tumors were injected with 1.6 μg Dbait-cy5.5/polyethylenimine (PEI) or 16 μg coDbait-cy5.5, excised and analyzed the following day for fluorescence distribution and DNA-PKcs activation. ( a ) Diffusion of fluorescent Dbait after two types of injections, intratumoral (IT) injection (A and B), or subcutaneous (SC) injections (C and D); white arrows indicate the sites of injection; scale: 2 mm. ( b ) DNA-PKcs activity was detected by H2AX phosphorylation (γ-H2AX) in tumors without treatment (NT) or after IT injection of Dbait/PEI or coDbait; left panels, cell nuclei stained with 4',6-diamidino-2-phenylindole (DAPI); right panels, immunodetection of γ-H2AX; scale, 20 μm.

    Journal: Cancer Gene Therapy

    Article Title: Comparison of distribution and activity of nanoparticles with short interfering DNA (Dbait) in various living systems

    doi: 10.1038/cgt.2011.39

    Figure Lengend Snippet: Diffusion and activity in tumors. Tumors were injected with 1.6 μg Dbait-cy5.5/polyethylenimine (PEI) or 16 μg coDbait-cy5.5, excised and analyzed the following day for fluorescence distribution and DNA-PKcs activation. ( a ) Diffusion of fluorescent Dbait after two types of injections, intratumoral (IT) injection (A and B), or subcutaneous (SC) injections (C and D); white arrows indicate the sites of injection; scale: 2 mm. ( b ) DNA-PKcs activity was detected by H2AX phosphorylation (γ-H2AX) in tumors without treatment (NT) or after IT injection of Dbait/PEI or coDbait; left panels, cell nuclei stained with 4',6-diamidino-2-phenylindole (DAPI); right panels, immunodetection of γ-H2AX; scale, 20 μm.

    Article Snippet: Linear polyethylenimine (PEI) (11 and 22 kDa) were obtained from Polyplus-Transfection (Illkirch, France) and provided as a ready-to-use solution of 300 m nitrogen concentration.

    Techniques: Diffusion-based Assay, Activity Assay, Injection, Fluorescence, Activation Assay, Staining, Immunodetection

    Non viral-mediated expression of transgenes in rat salivary glands . (A) Diagram of the transfection procedures in anesthetized rats. A thin polyethylene cannula is inserted into the Wharton's duct, as described in the Methods sections. The plasmid DNA is gently injected and diffuses into the large ducts (granular convoluted tubules or GCT, and the striated ducts or SD), then into the intercalated ducts (IC), and finally into the acinar canaliculi. Plasmid DNA accesses the epithelium through the apical plasma membrane (APM, arrows). (B) In vivo transfection with plasmid DNA -12 μg of Plasmid DNA encoding for pVenus were injected alone (CTRL, left panel), after SC injection of isoproterenol (ISO, center panel), or mixed with PEI as described in the Methods (PEI, right panel). After 16 h the submandibular SGs were excised and immediately imaged by two-photon microscopy using a 10X objective (excitation 930 nm) to reveal the cells expressing pVenus (arrows). Scale bar, 1 mm. (C) Effect of PEI on regulated exocytosis. Mice expressing cytoplasmic GFP were treated as described in B and the SGs imaged by confocal microscopy (excitation 488 nm) to estimate exocytosis of the secretory granules, as previously reported (Milberg et al., 2013 ). In the inset the apical plasma membrane (arrows) and the secretory granules (arrowheads) are highlighted. Scale bar, 20 μm. (D–E) PEI-mediated transfection in SGs drives expression into acinar cells. (D) Excised glands transfected with pVenus/PEI were imaged by two-photon microscopy using a 60x water immersion objective (D) or processed for immunofluorescence and imaged by confocal microscopy (E) . (D) Two Z-stacks from the same area of the sample were acquired twice by using two different excitation wavelengths, respectively: 740 nm to reveal the epithelium of the gland (cyan) and 930 nm to reveal pVenus (green) and collagen fibers (red). The Z-stacks were combined and shown as xz (left) or xy (center) projection. The cell expressing pVenus is part of an acinus (inset, right). Scale bar, 20 μm. (E) The SGs were labeled for phallodin (red) and the nuclear staining Hoechst (blue). The cell expressing pVenus (green) is part of an acinus (white broken line) as identified by the actin-labeled acinar canaliculi (arrowhead). Large ducts (yellow broken line), as identified by the characteristic actin pattern (asterisk) did not express any pVenus. Scale bar, 10 μm. (F) Diagram summarizing the pattern of expression of plasmid DNA in SGs. pVenus was expressed in cells of the IC under control conditions (left) and in acinar cells either upon stimulation with ISO (center) or administration with PEI (right).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Polyethylenimine-mediated expression of transgenes in the acinar cells of rats salivary glands in vivo

    doi: 10.3389/fcell.2014.00074

    Figure Lengend Snippet: Non viral-mediated expression of transgenes in rat salivary glands . (A) Diagram of the transfection procedures in anesthetized rats. A thin polyethylene cannula is inserted into the Wharton's duct, as described in the Methods sections. The plasmid DNA is gently injected and diffuses into the large ducts (granular convoluted tubules or GCT, and the striated ducts or SD), then into the intercalated ducts (IC), and finally into the acinar canaliculi. Plasmid DNA accesses the epithelium through the apical plasma membrane (APM, arrows). (B) In vivo transfection with plasmid DNA -12 μg of Plasmid DNA encoding for pVenus were injected alone (CTRL, left panel), after SC injection of isoproterenol (ISO, center panel), or mixed with PEI as described in the Methods (PEI, right panel). After 16 h the submandibular SGs were excised and immediately imaged by two-photon microscopy using a 10X objective (excitation 930 nm) to reveal the cells expressing pVenus (arrows). Scale bar, 1 mm. (C) Effect of PEI on regulated exocytosis. Mice expressing cytoplasmic GFP were treated as described in B and the SGs imaged by confocal microscopy (excitation 488 nm) to estimate exocytosis of the secretory granules, as previously reported (Milberg et al., 2013 ). In the inset the apical plasma membrane (arrows) and the secretory granules (arrowheads) are highlighted. Scale bar, 20 μm. (D–E) PEI-mediated transfection in SGs drives expression into acinar cells. (D) Excised glands transfected with pVenus/PEI were imaged by two-photon microscopy using a 60x water immersion objective (D) or processed for immunofluorescence and imaged by confocal microscopy (E) . (D) Two Z-stacks from the same area of the sample were acquired twice by using two different excitation wavelengths, respectively: 740 nm to reveal the epithelium of the gland (cyan) and 930 nm to reveal pVenus (green) and collagen fibers (red). The Z-stacks were combined and shown as xz (left) or xy (center) projection. The cell expressing pVenus is part of an acinus (inset, right). Scale bar, 20 μm. (E) The SGs were labeled for phallodin (red) and the nuclear staining Hoechst (blue). The cell expressing pVenus (green) is part of an acinus (white broken line) as identified by the actin-labeled acinar canaliculi (arrowhead). Large ducts (yellow broken line), as identified by the characteristic actin pattern (asterisk) did not express any pVenus. Scale bar, 10 μm. (F) Diagram summarizing the pattern of expression of plasmid DNA in SGs. pVenus was expressed in cells of the IC under control conditions (left) and in acinar cells either upon stimulation with ISO (center) or administration with PEI (right).

    Article Snippet: PEI-plasmid DNA complex Polyethyleneimine (PEI) was purchased from Polyplus Transfection (New York, NY) as in vivo -jet PEI.

    Techniques: Expressing, Transfection, Plasmid Preparation, Injection, In Vivo, Microscopy, Mouse Assay, Confocal Microscopy, Immunofluorescence, Labeling, Staining