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PolyScience polyethyleneimine
as an example) and on the thermally induced functionalization with oligomeric <t>polyethyleneimine</t> (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).
Polyethyleneimine, supplied by PolyScience, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Photoexcited State Properties of Carbon Dots from Thermally Induced Functionalization of Carbon Nanoparticles"

Article Title: Photoexcited State Properties of Carbon Dots from Thermally Induced Functionalization of Carbon Nanoparticles

Journal: Journal of materials chemistry. C, Materials for optical and electronic devices

doi: 10.1039/C6TC03666J

as an example) and on the thermally induced functionalization with oligomeric polyethyleneimine (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).
Figure Legend Snippet: as an example) and on the thermally induced functionalization with oligomeric polyethyleneimine (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).

Techniques Used:

Related Articles

In Vivo:

Article Title: Innate immunity mediated by dendritic cells/macrophages plays a central role in the early period in tumor treatment using gene of Mycobacterium tuberculosis antigen
Article Snippet: .. For in vivo transfection, the vehicle was constructed as described by Koyama et al. [ , ], by mixing the plasmid DNA with chondroitin sulfate sodium salt (CS: Seikagaku Corp., Tokyo, Japan) and polyethylenimine “Max” (PEI: Polyscience, Inc., Warrington, PA, U.S.A.): the DNA/CS/PEI complex. .. B16 cells (2 × 106 ) suspended in 0.1 m l Ca2+ , Mg2+ -free phosphate buffered saline, pH7.4, (PBS (−)) were injected subcutaneously into the backs of B6 mice.

Positive Control:

Article Title: Evaluation of Cell Penetrating Peptide Delivery System on HPV16E7 Expression in Three Types of Cell Line
Article Snippet: .. Polyethyleneimine (LINPEI 25 kDa, Polyscience, N/P: 7) was used as a positive control. .. The un-transfected cells were used as a negative control.

Molecular Weight:

Article Title: Photoexcited State Properties of Carbon Dots from Thermally Induced Functionalization of Carbon Nanoparticles
Article Snippet: .. The carbon nanopowder sample was purchased from US Research Nanomaterials, Inc., polyethyleneimine (PEI, branched, average molecular weight ~1,200) from Polyscience, Inc., and silicon carbide (120 Grit) from Panadyne Abrasives. .. Nitric acid was obtained from Fisher Scientific, and deuterated water for NMR experiments from Cambridge Isotope Laboratories.

Article Title: Modified Facile Synthesis for Quantitatively Fluorescent Carbon Dots
Article Snippet: .. Citric acid was purchased from Alfa Aesar, and polyethylenimine (PEI, branched, average molecular weight ~1,200) from Polyscience, Inc. .. The dialysis membrane tubing (molecular weight cut-off ~ 500 or 1,000) was supplied by Spectrum Laboratories.

Transfection:

Article Title: Artificial Induction of Native Aquaporin-1 Expression in Human Salivary Cells
Article Snippet: .. For A253 cell transfection, polyethylenimine (PEI; Polyscience) polymer was used because the cells were permissive. .. For hS/PCs, Lipofectamine 2000 (Thermo Fisher Scientific) was used to enhance transfection efficiency.

Article Title: Cell-intrinsic determinants of ibrutinib-induced apoptosis in Chronic Lymphocytic Leukemia
Article Snippet: .. HEK293T cells were transfected with recombinant pLentiCRISPRv2 together with viral packaging plasmids using the polyethylenimine (PEI; Polyscience Inc., #23966) transfection protocol. .. Viral supernatant fractions were collected 48–72 h after transfection by low speed centrifugation to remove cells and debris.

Article Title: Innate immunity mediated by dendritic cells/macrophages plays a central role in the early period in tumor treatment using gene of Mycobacterium tuberculosis antigen
Article Snippet: .. For in vivo transfection, the vehicle was constructed as described by Koyama et al. [ , ], by mixing the plasmid DNA with chondroitin sulfate sodium salt (CS: Seikagaku Corp., Tokyo, Japan) and polyethylenimine “Max” (PEI: Polyscience, Inc., Warrington, PA, U.S.A.): the DNA/CS/PEI complex. .. B16 cells (2 × 106 ) suspended in 0.1 m l Ca2+ , Mg2+ -free phosphate buffered saline, pH7.4, (PBS (−)) were injected subcutaneously into the backs of B6 mice.

Article Title: Dual roles of the Sterol Recognition Region in Hedgehog protein modification
Article Snippet: .. Polyethylenimine (PEI, PolyScience, 23996-1) (1 mg/mL stock) was used as a transfection reagent. .. Analysis of hSHH protein from cells HEK-293T cells were seeded in a 12-well plate 24 h before transfection at a density of 200,000 cells/well.

Construct:

Article Title: Innate immunity mediated by dendritic cells/macrophages plays a central role in the early period in tumor treatment using gene of Mycobacterium tuberculosis antigen
Article Snippet: .. For in vivo transfection, the vehicle was constructed as described by Koyama et al. [ , ], by mixing the plasmid DNA with chondroitin sulfate sodium salt (CS: Seikagaku Corp., Tokyo, Japan) and polyethylenimine “Max” (PEI: Polyscience, Inc., Warrington, PA, U.S.A.): the DNA/CS/PEI complex. .. B16 cells (2 × 106 ) suspended in 0.1 m l Ca2+ , Mg2+ -free phosphate buffered saline, pH7.4, (PBS (−)) were injected subcutaneously into the backs of B6 mice.

Incubation:

Article Title: MicroRNA-21 induces loss of 15-hydroxyprostaglandin dehydrogenase in early gastric tubular adenocarcinoma
Article Snippet: .. Knock-out viral vectors (Viral vector: PLP1: PLP2: VSV-G) were mixed at a 9:6:6:3 (μg) ratio with 3-fold polyethyleneimine (PEI, 1 μg/mL Polyscience), and then incubated at RT for 20 min. After incubation, the pre-made mixture was added to 293 T cells for 16 h and then replaced with growth medium. .. After incubation for 16 h, the media soup was collected and filtrated using a 0.45 μm PVDF membrane syringe filter (Millipore, Bedford, MA, USA).

Knock-Out:

Article Title: MicroRNA-21 induces loss of 15-hydroxyprostaglandin dehydrogenase in early gastric tubular adenocarcinoma
Article Snippet: .. Knock-out viral vectors (Viral vector: PLP1: PLP2: VSV-G) were mixed at a 9:6:6:3 (μg) ratio with 3-fold polyethyleneimine (PEI, 1 μg/mL Polyscience), and then incubated at RT for 20 min. After incubation, the pre-made mixture was added to 293 T cells for 16 h and then replaced with growth medium. .. After incubation for 16 h, the media soup was collected and filtrated using a 0.45 μm PVDF membrane syringe filter (Millipore, Bedford, MA, USA).

Recombinant:

Article Title: Cell-intrinsic determinants of ibrutinib-induced apoptosis in Chronic Lymphocytic Leukemia
Article Snippet: .. HEK293T cells were transfected with recombinant pLentiCRISPRv2 together with viral packaging plasmids using the polyethylenimine (PEI; Polyscience Inc., #23966) transfection protocol. .. Viral supernatant fractions were collected 48–72 h after transfection by low speed centrifugation to remove cells and debris.

Plasmid Preparation:

Article Title: Innate immunity mediated by dendritic cells/macrophages plays a central role in the early period in tumor treatment using gene of Mycobacterium tuberculosis antigen
Article Snippet: .. For in vivo transfection, the vehicle was constructed as described by Koyama et al. [ , ], by mixing the plasmid DNA with chondroitin sulfate sodium salt (CS: Seikagaku Corp., Tokyo, Japan) and polyethylenimine “Max” (PEI: Polyscience, Inc., Warrington, PA, U.S.A.): the DNA/CS/PEI complex. .. B16 cells (2 × 106 ) suspended in 0.1 m l Ca2+ , Mg2+ -free phosphate buffered saline, pH7.4, (PBS (−)) were injected subcutaneously into the backs of B6 mice.

Article Title: MicroRNA-21 induces loss of 15-hydroxyprostaglandin dehydrogenase in early gastric tubular adenocarcinoma
Article Snippet: .. Knock-out viral vectors (Viral vector: PLP1: PLP2: VSV-G) were mixed at a 9:6:6:3 (μg) ratio with 3-fold polyethyleneimine (PEI, 1 μg/mL Polyscience), and then incubated at RT for 20 min. After incubation, the pre-made mixture was added to 293 T cells for 16 h and then replaced with growth medium. .. After incubation for 16 h, the media soup was collected and filtrated using a 0.45 μm PVDF membrane syringe filter (Millipore, Bedford, MA, USA).

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  • 92
    PolyScience polyethyleneimine
    as an example) and on the thermally induced functionalization with oligomeric <t>polyethyleneimine</t> (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).
    Polyethyleneimine, supplied by PolyScience, used in various techniques. Bioz Stars score: 92/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine/product/PolyScience
    Average 92 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine - by Bioz Stars, 2020-08
    92/100 stars
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    as an example) and on the thermally induced functionalization with oligomeric polyethyleneimine (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).

    Journal: Journal of materials chemistry. C, Materials for optical and electronic devices

    Article Title: Photoexcited State Properties of Carbon Dots from Thermally Induced Functionalization of Carbon Nanoparticles

    doi: 10.1039/C6TC03666J

    Figure Lengend Snippet: as an example) and on the thermally induced functionalization with oligomeric polyethyleneimine (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).

    Article Snippet: The carbon nanopowder sample was purchased from US Research Nanomaterials, Inc., polyethyleneimine (PEI, branched, average molecular weight ~1,200) from Polyscience, Inc., and silicon carbide (120 Grit) from Panadyne Abrasives.

    Techniques:

    Effect of over-expression and knockdown of Hs MoT2/MFSD5 in molybdate uptake rate. A , Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the polyethyleneimine method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.

    Journal: PLoS ONE

    Article Title: Exploring Dynamics of Molybdate in Living Animal Cells by a Genetically Encoded FRET Nanosensor

    doi: 10.1371/journal.pone.0058175

    Figure Lengend Snippet: Effect of over-expression and knockdown of Hs MoT2/MFSD5 in molybdate uptake rate. A , Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the polyethyleneimine method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.

    Article Snippet: Co-transfection of cells with pYN723 and pYN769 were performed by the polyethyleneimine transfection method using 25 kD linear- polyethyleneimine (Polyscience) , .

    Techniques: Over Expression, Transfection, Plasmid Preparation, Fluorescence, Expressing

    A cartoon illustration on carbon dot, defined generally as a small carbon nanoparticle core with surface passivation species (a configuration similar to a soft corona), which in this work are likely zwitterionic pairs of citric acid and amino groups on polyethylenimine and the corresponding amides upon dehydration.

    Journal: Carbon

    Article Title: Modified Facile Synthesis for Quantitatively Fluorescent Carbon Dots

    doi: 10.1016/j.carbon.2017.06.093

    Figure Lengend Snippet: A cartoon illustration on carbon dot, defined generally as a small carbon nanoparticle core with surface passivation species (a configuration similar to a soft corona), which in this work are likely zwitterionic pairs of citric acid and amino groups on polyethylenimine and the corresponding amides upon dehydration.

    Article Snippet: Citric acid was purchased from Alfa Aesar, and polyethylenimine (PEI, branched, average molecular weight ~1,200) from Polyscience, Inc.

    Techniques: