Structured Review

Millipore polyethersulfone membrane
Exogenous expression of MFNs enhance the PerMit Venus reporter signal. a Schematic for the constructs of Cyto-V(N), Mito-V(N), and Po-V(C). Cyto-V(N), HA tag fused to the N terminus of Venus with a linker composed of 4 × GGSG (indicated with blue box); Mito-V(N), Tom20 fused to the N terminus Venus with a HA tag and two linkers as indicated; Po-V(C), Myc tag and PEX26(residues 237-305) fused to the C terminus of Venus (residues 155-238). b Immuno-blots for Cyto-V(N), Mito-V(N), and Po-V(C). c Immunofluorescence images of Cyto-V(N), Mito-V(N), and Po-V(C) co-stained with mitochondrial COX4 and peroxisomal ABCD3 in HeLa cells. Scale bars, 5 μm. d Immunofluorescence images of Venus co-stained with mitochondrial COX4 and peroxisomal ABCD3 in PerMit Venus and control cells. Scale bars, 5 μm. e Immunofluorescence images of PerMit Venus cells with exogenously expressed MFNs. PerMit Venus cells were transfected with empty vector, MFN1-FLAG, or MFN2-FLAG plasmids for 36 h. FLAG (Alexa Fluor 568) and peroxisomal <t>membrane</t> protein PEX14 (Alexa Fluor 647) were immunostained. Scale bars, 5 μm. f Integrated density of ( e ), Vector, n = 67; MFN1-FLAG, n = 73; MFN2-FLAG, n = 72. *** p
Polyethersulfone Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyethersulfone membrane/product/Millipore
Average 99 stars, based on 40 article reviews
Price from $9.99 to $1999.99
polyethersulfone membrane - by Bioz Stars, 2022-09
99/100 stars

Images

1) Product Images from "The MFN1 and MFN2 mitofusins promote clustering between mitochondria and peroxisomes"

Article Title: The MFN1 and MFN2 mitofusins promote clustering between mitochondria and peroxisomes

Journal: Communications Biology

doi: 10.1038/s42003-022-03377-x

Exogenous expression of MFNs enhance the PerMit Venus reporter signal. a Schematic for the constructs of Cyto-V(N), Mito-V(N), and Po-V(C). Cyto-V(N), HA tag fused to the N terminus of Venus with a linker composed of 4 × GGSG (indicated with blue box); Mito-V(N), Tom20 fused to the N terminus Venus with a HA tag and two linkers as indicated; Po-V(C), Myc tag and PEX26(residues 237-305) fused to the C terminus of Venus (residues 155-238). b Immuno-blots for Cyto-V(N), Mito-V(N), and Po-V(C). c Immunofluorescence images of Cyto-V(N), Mito-V(N), and Po-V(C) co-stained with mitochondrial COX4 and peroxisomal ABCD3 in HeLa cells. Scale bars, 5 μm. d Immunofluorescence images of Venus co-stained with mitochondrial COX4 and peroxisomal ABCD3 in PerMit Venus and control cells. Scale bars, 5 μm. e Immunofluorescence images of PerMit Venus cells with exogenously expressed MFNs. PerMit Venus cells were transfected with empty vector, MFN1-FLAG, or MFN2-FLAG plasmids for 36 h. FLAG (Alexa Fluor 568) and peroxisomal membrane protein PEX14 (Alexa Fluor 647) were immunostained. Scale bars, 5 μm. f Integrated density of ( e ), Vector, n = 67; MFN1-FLAG, n = 73; MFN2-FLAG, n = 72. *** p
Figure Legend Snippet: Exogenous expression of MFNs enhance the PerMit Venus reporter signal. a Schematic for the constructs of Cyto-V(N), Mito-V(N), and Po-V(C). Cyto-V(N), HA tag fused to the N terminus of Venus with a linker composed of 4 × GGSG (indicated with blue box); Mito-V(N), Tom20 fused to the N terminus Venus with a HA tag and two linkers as indicated; Po-V(C), Myc tag and PEX26(residues 237-305) fused to the C terminus of Venus (residues 155-238). b Immuno-blots for Cyto-V(N), Mito-V(N), and Po-V(C). c Immunofluorescence images of Cyto-V(N), Mito-V(N), and Po-V(C) co-stained with mitochondrial COX4 and peroxisomal ABCD3 in HeLa cells. Scale bars, 5 μm. d Immunofluorescence images of Venus co-stained with mitochondrial COX4 and peroxisomal ABCD3 in PerMit Venus and control cells. Scale bars, 5 μm. e Immunofluorescence images of PerMit Venus cells with exogenously expressed MFNs. PerMit Venus cells were transfected with empty vector, MFN1-FLAG, or MFN2-FLAG plasmids for 36 h. FLAG (Alexa Fluor 568) and peroxisomal membrane protein PEX14 (Alexa Fluor 647) were immunostained. Scale bars, 5 μm. f Integrated density of ( e ), Vector, n = 67; MFN1-FLAG, n = 73; MFN2-FLAG, n = 72. *** p

Techniques Used: Expressing, Construct, Western Blot, Immunofluorescence, Staining, Transfection, Plasmid Preparation

Exogenous expression of MFN induces peroxisome/mitochondrion clustering. Immunofluorescence images of overexpressed MFN-EGFP. HeLa cells were transfected with free EGFP ( a ) or EGFP fused MFN ( b ) plasmids for 36 h. Peroxisomal matrix protein catalase (Alexa Fluor 555) and peroxisomal membrane protein ABCD3 (Alexa Fluor 647) were immune-stained. Scale bars, 5 μm. c Peroxisomal membrane protein PEX14 (Alexa Fluor 555) and outer mitochondrial membrane protein Tom20 (Alexa Fluor 647) were immunostained with or without exogenously expressed MFNs. Scale bars, 5 μm. d Immuno-blots of overexpressed MFN1-EGFP and MFN2-EGFP. 1.5 µg plasmids were transfected into HeLa cells in one well in a six-well cell culture plate for 36 h and immunoblotted with indicated antibodies. e Immunofluorescence images of overexpressed MFN2-EGFP and other organelle markers. HeLa cells were transfected with MFN2-EGFP and immunostained for peroxisomal membrane protein ABCD3 (Alexa Fluor 647) and other organelle markers (Alexa Fluor 555): calnexin (endoplasmic reticulum), EEA1 (early endosome), GM130 (Golgi), and LAMP1 (lysosome). Scale bars, 5 μm. f Immunofluorescence images of overexpressed MFN1-EGFP and other organelle markers stained the same as in ( c ).
Figure Legend Snippet: Exogenous expression of MFN induces peroxisome/mitochondrion clustering. Immunofluorescence images of overexpressed MFN-EGFP. HeLa cells were transfected with free EGFP ( a ) or EGFP fused MFN ( b ) plasmids for 36 h. Peroxisomal matrix protein catalase (Alexa Fluor 555) and peroxisomal membrane protein ABCD3 (Alexa Fluor 647) were immune-stained. Scale bars, 5 μm. c Peroxisomal membrane protein PEX14 (Alexa Fluor 555) and outer mitochondrial membrane protein Tom20 (Alexa Fluor 647) were immunostained with or without exogenously expressed MFNs. Scale bars, 5 μm. d Immuno-blots of overexpressed MFN1-EGFP and MFN2-EGFP. 1.5 µg plasmids were transfected into HeLa cells in one well in a six-well cell culture plate for 36 h and immunoblotted with indicated antibodies. e Immunofluorescence images of overexpressed MFN2-EGFP and other organelle markers. HeLa cells were transfected with MFN2-EGFP and immunostained for peroxisomal membrane protein ABCD3 (Alexa Fluor 647) and other organelle markers (Alexa Fluor 555): calnexin (endoplasmic reticulum), EEA1 (early endosome), GM130 (Golgi), and LAMP1 (lysosome). Scale bars, 5 μm. f Immunofluorescence images of overexpressed MFN1-EGFP and other organelle markers stained the same as in ( c ).

Techniques Used: Expressing, Immunofluorescence, Transfection, Staining, Western Blot, Cell Culture

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  • 99
    Millipore steritop vacuum sterile polyethersulfone pes membrane
    Performance of 0.22 µm filtration and its impact on HFDa fraction (referred as HFDa 0.22 µm ). (a) Microbiological testing of fractions HFDa 0.22 µm experiment performed in triplicate; (b) SDS-PAGE stained with colloidal Coomassie; (c) western blots with anti-ALIX, anti-TSGl0l; and (d) anti-DPPlV, anti-CD63; (e) PicoChip, (f) Small Chip electropherograms from HFDa 0.22 µm RNA extracted with Norgen Kit; ST-molecular weight marker, 1,2,3 – HFDa 0.22 µm fractions (triplicate), 4,5,6 – SDS elution from <t>Steritop</t> filters (triplicate), kDa – kilodalton.
    Steritop Vacuum Sterile Polyethersulfone Pes Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steritop vacuum sterile polyethersulfone pes membrane/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    steritop vacuum sterile polyethersulfone pes membrane - by Bioz Stars, 2022-09
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    90
    Millipore piece of polyethersulfone pes membrane
    Schematic overview <t>of</t> FLASH (Fast, Low-cost, Aerosol-contamination-free and Sensitive molecular assay for at-Home tests of communicable pathogens) workflow. 1. Saliva or transport medium for nasopharyngeal swabs is loaded into the SampleDirect-Pen containing lyophilized extraction reagents and flows through the device by pressing. Released nucleic acids are captured by a <t>polyethersulfone</t> <t>(PES)</t> membrane. 2. The membrane is transferred to the Detection-Pen, where the oLAMP reaction is carried and terminated by mixing with assay buffer. 3. The lateral-flow strip in Detection-Pen is inserted into the solution for visible readout by naked-eye or interpreted by a smartphone app.
    Piece Of Polyethersulfone Pes Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/piece of polyethersulfone pes membrane/product/Millipore
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    piece of polyethersulfone pes membrane - by Bioz Stars, 2022-09
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    97
    Millipore 5 kda mwco membrane
    Silicibacter sp. strain TrichCH4B biological response to NO and Trichodesmium erythraeum . (A) Exogenous NO increases Silicibacter sp. TrichCH4B biofilm formation. Static biofilm assays were performed as described in Materials and Methods. With increasing amounts of NO, Silicibacter sp. TrichCH4B formed more biofilm, as quantified by crystal violet staining (OD 570 ). Biofilm formation was normalized against growth (OD 600 ). (B) Addition of T. erythraeum spent medium (TSM) increases Silicibacter sp. TrichCH4B NO formation. Silicibacter sp. TrichCH4B was grown anaerobically with TSM as described in Materials and Methods. To fully digest any proteins, TSM was also treated with proteinase K before the addition to Silicibacter sp. TrichCH4B. Only the TSM (high-molecular-weight [MW] fraction, or retentate, from a <t>5-kDa-MWCO</t> membrane filter) showed stimulation of NO formation by Silicibacter sp. TrichCH4B. (C) TSM addition increases SiliNOS gene expression. Silicibacter sp. TrichCH4B was grown aerobically with various amounts of TSM, and cDNA from Silicibacter sp. TrichCH4B mRNA was prepared as described in Materials and Methods. Expression of the SiliNOS gene (ΔC(t)) was calculated using Bio-Rad CFX Manager software with rpoD as a reference gene. SiliNOS gene expression increased with the amount of TSM added. (D) TSM addition increases Silicibacter sp. TrichCH4B biofilm formation. Static biofilm assays were performed as described in Materials and Methods. Silicibacter sp. TrichCH4B biofilm formation increased with the amount of TSM added, as quantified by crystal violet staining. Values that are significantly different are indicated by bars and asterisks as follows: *, P
    5 Kda Mwco Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Performance of 0.22 µm filtration and its impact on HFDa fraction (referred as HFDa 0.22 µm ). (a) Microbiological testing of fractions HFDa 0.22 µm experiment performed in triplicate; (b) SDS-PAGE stained with colloidal Coomassie; (c) western blots with anti-ALIX, anti-TSGl0l; and (d) anti-DPPlV, anti-CD63; (e) PicoChip, (f) Small Chip electropherograms from HFDa 0.22 µm RNA extracted with Norgen Kit; ST-molecular weight marker, 1,2,3 – HFDa 0.22 µm fractions (triplicate), 4,5,6 – SDS elution from Steritop filters (triplicate), kDa – kilodalton.

    Journal: Journal of Extracellular Vesicles

    Article Title: Urinary extracellular vesicles for RNA extraction: optimization of a protocol devoid of prokaryote contamination

    doi: 10.3402/jev.v5.30281

    Figure Lengend Snippet: Performance of 0.22 µm filtration and its impact on HFDa fraction (referred as HFDa 0.22 µm ). (a) Microbiological testing of fractions HFDa 0.22 µm experiment performed in triplicate; (b) SDS-PAGE stained with colloidal Coomassie; (c) western blots with anti-ALIX, anti-TSGl0l; and (d) anti-DPPlV, anti-CD63; (e) PicoChip, (f) Small Chip electropherograms from HFDa 0.22 µm RNA extracted with Norgen Kit; ST-molecular weight marker, 1,2,3 – HFDa 0.22 µm fractions (triplicate), 4,5,6 – SDS elution from Steritop filters (triplicate), kDa – kilodalton.

    Article Snippet: In the alternative approach, supernatant 2,000 g (triplicate of 500 ml) with sodium citrate/EDTA addition was filtered through Steritop vacuum sterile polyethersulfone (PES) membrane (0.22 µm pore size; Millipore) using 700 mbar vacuum pressure by WOB-L Pressure/Vacuum Pump model no. 2522C-02 (Welch, Sheboygan, WI).

    Techniques: Filtration, SDS Page, Staining, Western Blot, Chromatin Immunoprecipitation, Molecular Weight, Marker

    Effect of pH of growth medium and rpoS genotype on surface-associated acid tolerance of Salmonella serovar Typhimurium. The wild-type (14028s) and rpoS mutant (SF1005) cells were grown under adaptive (LB-MES; pH 5.5) or unadaptive (LB-MOPS; pH 8.0) growth conditions and were acid challenged either after inoculation onto polyethersulfone membranes or in the planktonic state. The error bars indicate standard deviation (not shown where smaller than the line thickness).

    Journal: Applied and Environmental Microbiology

    Article Title: Inoculation onto Solid Surfaces Protects Salmonella spp. during Acid Challenge: a Model Study Using Polyethersulfone Membranes

    doi: 10.1128/AEM.68.1.86-92.2002

    Figure Lengend Snippet: Effect of pH of growth medium and rpoS genotype on surface-associated acid tolerance of Salmonella serovar Typhimurium. The wild-type (14028s) and rpoS mutant (SF1005) cells were grown under adaptive (LB-MES; pH 5.5) or unadaptive (LB-MOPS; pH 8.0) growth conditions and were acid challenged either after inoculation onto polyethersulfone membranes or in the planktonic state. The error bars indicate standard deviation (not shown where smaller than the line thickness).

    Article Snippet: Polyethersulfone membranes (0.22 μm thick) (Durapore membrane; Millipore Inc., Bedford, Mass.) were washed in distilled water for 1 h and dried at room temperature before they were laid on water agar plates (1.6% agar [wt/vol] in distilled water).

    Techniques: Mutagenesis, Standard Deviation

    Schematic overview of FLASH (Fast, Low-cost, Aerosol-contamination-free and Sensitive molecular assay for at-Home tests of communicable pathogens) workflow. 1. Saliva or transport medium for nasopharyngeal swabs is loaded into the SampleDirect-Pen containing lyophilized extraction reagents and flows through the device by pressing. Released nucleic acids are captured by a polyethersulfone (PES) membrane. 2. The membrane is transferred to the Detection-Pen, where the oLAMP reaction is carried and terminated by mixing with assay buffer. 3. The lateral-flow strip in Detection-Pen is inserted into the solution for visible readout by naked-eye or interpreted by a smartphone app.

    Journal: Biosensors & Bioelectronics

    Article Title: Disposable and low-cost pen-like sensor incorporating nucleic-acid amplification based lateral-flow assay for at-home tests of communicable pathogens

    doi: 10.1016/j.biosx.2022.100248

    Figure Lengend Snippet: Schematic overview of FLASH (Fast, Low-cost, Aerosol-contamination-free and Sensitive molecular assay for at-Home tests of communicable pathogens) workflow. 1. Saliva or transport medium for nasopharyngeal swabs is loaded into the SampleDirect-Pen containing lyophilized extraction reagents and flows through the device by pressing. Released nucleic acids are captured by a polyethersulfone (PES) membrane. 2. The membrane is transferred to the Detection-Pen, where the oLAMP reaction is carried and terminated by mixing with assay buffer. 3. The lateral-flow strip in Detection-Pen is inserted into the solution for visible readout by naked-eye or interpreted by a smartphone app.

    Article Snippet: To fabricate the SampleDirect-Pen, a piece of polyethersulfone (PES) membrane (0.22 μm pore size, 2-mm diameter) (Millipore) was compressed onto the tip of a 1-mL syringe (Becton Dickinson) (see sample preparation) and secured by a 3D-printed cap.

    Techniques: Stripping Membranes

    Silicibacter sp. strain TrichCH4B biological response to NO and Trichodesmium erythraeum . (A) Exogenous NO increases Silicibacter sp. TrichCH4B biofilm formation. Static biofilm assays were performed as described in Materials and Methods. With increasing amounts of NO, Silicibacter sp. TrichCH4B formed more biofilm, as quantified by crystal violet staining (OD 570 ). Biofilm formation was normalized against growth (OD 600 ). (B) Addition of T. erythraeum spent medium (TSM) increases Silicibacter sp. TrichCH4B NO formation. Silicibacter sp. TrichCH4B was grown anaerobically with TSM as described in Materials and Methods. To fully digest any proteins, TSM was also treated with proteinase K before the addition to Silicibacter sp. TrichCH4B. Only the TSM (high-molecular-weight [MW] fraction, or retentate, from a 5-kDa-MWCO membrane filter) showed stimulation of NO formation by Silicibacter sp. TrichCH4B. (C) TSM addition increases SiliNOS gene expression. Silicibacter sp. TrichCH4B was grown aerobically with various amounts of TSM, and cDNA from Silicibacter sp. TrichCH4B mRNA was prepared as described in Materials and Methods. Expression of the SiliNOS gene (ΔC(t)) was calculated using Bio-Rad CFX Manager software with rpoD as a reference gene. SiliNOS gene expression increased with the amount of TSM added. (D) TSM addition increases Silicibacter sp. TrichCH4B biofilm formation. Static biofilm assays were performed as described in Materials and Methods. Silicibacter sp. TrichCH4B biofilm formation increased with the amount of TSM added, as quantified by crystal violet staining. Values that are significantly different are indicated by bars and asterisks as follows: *, P

    Journal: mBio

    Article Title: Nitric Oxide Mediates Biofilm Formation and Symbiosis in Silicibacter sp. Strain TrichCH4B

    doi: 10.1128/mBio.00206-15

    Figure Lengend Snippet: Silicibacter sp. strain TrichCH4B biological response to NO and Trichodesmium erythraeum . (A) Exogenous NO increases Silicibacter sp. TrichCH4B biofilm formation. Static biofilm assays were performed as described in Materials and Methods. With increasing amounts of NO, Silicibacter sp. TrichCH4B formed more biofilm, as quantified by crystal violet staining (OD 570 ). Biofilm formation was normalized against growth (OD 600 ). (B) Addition of T. erythraeum spent medium (TSM) increases Silicibacter sp. TrichCH4B NO formation. Silicibacter sp. TrichCH4B was grown anaerobically with TSM as described in Materials and Methods. To fully digest any proteins, TSM was also treated with proteinase K before the addition to Silicibacter sp. TrichCH4B. Only the TSM (high-molecular-weight [MW] fraction, or retentate, from a 5-kDa-MWCO membrane filter) showed stimulation of NO formation by Silicibacter sp. TrichCH4B. (C) TSM addition increases SiliNOS gene expression. Silicibacter sp. TrichCH4B was grown aerobically with various amounts of TSM, and cDNA from Silicibacter sp. TrichCH4B mRNA was prepared as described in Materials and Methods. Expression of the SiliNOS gene (ΔC(t)) was calculated using Bio-Rad CFX Manager software with rpoD as a reference gene. SiliNOS gene expression increased with the amount of TSM added. (D) TSM addition increases Silicibacter sp. TrichCH4B biofilm formation. Static biofilm assays were performed as described in Materials and Methods. Silicibacter sp. TrichCH4B biofilm formation increased with the amount of TSM added, as quantified by crystal violet staining. Values that are significantly different are indicated by bars and asterisks as follows: *, P

    Article Snippet: Spent medium from 14 to 28 days of T. erythraeum culture growth was collected by filtration through a 0.2-µm filter and then concentrated 1,000 times by tangential-flow filtration with a 5-kDa MWCO membrane (Millipore).

    Techniques: Staining, Molecular Weight, Expressing, Software