polyclonal rabbit anti ing3 antibody  (Proteintech)

Journal: Oncology Letters

Article Title: Downregulation of inhibitor of growth 3 is correlated with tumorigenesis and progression of hepatocellular carcinoma

doi: 10.3892/ol.2012.685

Figure Lengend Snippet: Expression pattern of ING3 in HCC samples and cell lines. (A) The transcript level of ING3 was measured in 49 paired HCC and adjacent non-cancerous livers by quantitative RT-PCR, where GAPDH was used as an internal reference. The line within each box is the median -ΔCt value; the upper and lower edges of each box show the 75th and 25th percentile, respectively. The upper and lower bars are the highest and lowest values. P-value was calculated by paired sample t-test (p<0.001). (B) Representative semi-quantitative RT-PCR of ING3 of 3 pairs of HCC samples. C, cancer tissue; N, adjacent noncancerous liver. (C) Relative mRNA level of ING3 was evaluated in HCC cell lines and fetal liver by semiquantitative RT-PCR. HCC, hepatocellular carcinoma; ING3, inhibitor of growth 3; RT-PCR, reverse transcription-polymerase chain reaction.

Article Snippet: The slides were incubated overnight at 4°C with rabbit anti-ING3 polyclonal antibody (1:200 dilution), followed by incubation with a horseradish peroxidase-conjugated anti-rabbit secondary antibody (Dako Japan Ltd., Kyoto, Japan) at 37°C for 30 min. Normal rabbit IgG was used as a negative control.

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Downregulation of inhibitor of growth 3 is correlated with tumorigenesis and progression of hepatocellular carcinoma

doi: 10.3892/ol.2012.685

Figure Lengend Snippet: Representative immunohistochemical detection of the ING3 protein expression in HCC and surrounding noncancerous tissues. HCC, hepatocellular carcinoma; ING3, inhibitor of growth 3.

Article Snippet: The slides were incubated overnight at 4°C with rabbit anti-ING3 polyclonal antibody (1:200 dilution), followed by incubation with a horseradish peroxidase-conjugated anti-rabbit secondary antibody (Dako Japan Ltd., Kyoto, Japan) at 37°C for 30 min. Normal rabbit IgG was used as a negative control.

Techniques: Immunohistochemical staining, Expressing

Journal: Oncology Letters

Article Title: Downregulation of inhibitor of growth 3 is correlated with tumorigenesis and progression of hepatocellular carcinoma

doi: 10.3892/ol.2012.685

Figure Lengend Snippet: Correlations between ING3 expression and clinicopathological variables of 112 cases of HCC.

Article Snippet: The slides were incubated overnight at 4°C with rabbit anti-ING3 polyclonal antibody (1:200 dilution), followed by incubation with a horseradish peroxidase-conjugated anti-rabbit secondary antibody (Dako Japan Ltd., Kyoto, Japan) at 37°C for 30 min. Normal rabbit IgG was used as a negative control.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Downregulation of inhibitor of growth 3 is correlated with tumorigenesis and progression of hepatocellular carcinoma

doi: 10.3892/ol.2012.685

Figure Lengend Snippet: ING3 overexpression inhibits cell proliferation and colony formation. (A) Exogenous ING3 was expressed in Hep3B and Huh7 cells by transient transfection with pcDNA3.0-ING3, confirmed by western blot analysis. Cells transfected with an empty vector were used as the control. (B) The growth curve of Hep3B cells with the exogenous ING3. Cells transfected with the empty vector pcDNA3.0 served as controls. The experiments were repeated three times and the spots represent the average values of triplicate wells, with SD included for each mean value. (C) ING3 suppressed the ability of Hep3B cells to form colonies. The relative numbers of colonies of Hep3B cells transfected with pcDNA3.0-ING3 and a control vector were calculated from three independent experiments (***p<0.01). The lower panel showed the representative dishes of the experiments. (D) ING3 inhibited the colony formation of Huh7 cells (*p<0.05 as compared with the control vectors). ING3, inhibitor of growth 3.

Article Snippet: The slides were incubated overnight at 4°C with rabbit anti-ING3 polyclonal antibody (1:200 dilution), followed by incubation with a horseradish peroxidase-conjugated anti-rabbit secondary antibody (Dako Japan Ltd., Kyoto, Japan) at 37°C for 30 min. Normal rabbit IgG was used as a negative control.

Techniques: Over Expression, Transfection, Western Blot, Plasmid Preparation

Journal: Oncology Letters

Article Title: Downregulation of inhibitor of growth 3 is correlated with tumorigenesis and progression of hepatocellular carcinoma

doi: 10.3892/ol.2012.685

Figure Lengend Snippet: ING3 inhibits the migration of hepatoma cells. (A and B) ING3 overexpression reduced the migration of (A) Hep3B and (B) Huh7 cells. The two cell types were scraped with micropipette tips following transfection with pcDNA3.0-ING3 or pcDNA3.0. Images were captured at 0, 24 and 48 h following wounding. Cells transfected with an empty vector were used as controls. (C and D) The wound healing rates at 24 and 48 h following wounding of (C) Hep3B and (D) Huh7 cells. Values are the mean ± SD. *p<0.05. ING3, inhibitor of growth 3.

Article Snippet: The slides were incubated overnight at 4°C with rabbit anti-ING3 polyclonal antibody (1:200 dilution), followed by incubation with a horseradish peroxidase-conjugated anti-rabbit secondary antibody (Dako Japan Ltd., Kyoto, Japan) at 37°C for 30 min. Normal rabbit IgG was used as a negative control.

Techniques: Migration, Over Expression, Transfection, Plasmid Preparation