polyclonal rabbit anti connexin 43 cx43  (Millipore)


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    Name:
    Anti Connexin 43 antibody
    Description:
    Anti Connexin 43 is developed in rabbit using a synthetic peptide conjugated to KLH with glutaraldehyde as immunogen The peptide corresponds to a C terminal segment of the cytoplasmic domain of human and rat connexin 43 amino acid residues with an N terminal added lysine Affinity isolated antigen specific antibody is obtained by immunospecific purification which removes essentially all rabbit serum proteins including immunoglobulins which do not specifically bind to connexin 43 Gap junctions are specialized cell membrane domains consisting of aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells electrical coupling synchronization of cellular physiological activities growth control and developmental regulation The gap junction channels allow intercellular exchange of ions nucleotides and small molecules between adjacent cells Unlike other membrane channels intercellular channels span two opposed plasma membranes and require the contribution of hemi channels called connexons from both participating cells These channels are permeable to molecules as large as 1 kDa and they have been reported in most mammalian cell types Two connexons interact in the extracellular space to form the complete intercellular channel Each connexon is composed of six similar or identical proteins which have been termed connexins Connexins Cx are a multi gene family of highly related proteins with molecular weights ranging from 26 to 70 kDa At least a dozen distinct connexin genes have been identified in mammals many expressed in a diverse tissue and cell specific pattern Two distinct lineages have been identified in mammals one termed class I or β group in which Cx26 Cx30 Cx31 Cx31 1 and Cx32 fall and the other termed class II or α group represented by Cx33 Cx37 Cx40 Cx43 and Cx46 Connexin 43 Cx 43 a 43kDa protein is a member of the connexin protein family that forms a gap junction It is expressed by a variety of cell types such as astrocytes cardiac and smooth muscle endothelium ependyma fibroblasts keratinocytes lens and corneal epithelium In addition the protein is also found in leptomeninges leucocytes Leydig cells macrophages myoepithelial cells of mammary gland osteocytes ovarian granulosa pancreatic β cells preimplantation blastocyst sertoli cells thyroid follicular cells and trophoblast giant cells
    Catalog Number:
    c6219
    Price:
    None
    Applications:
    Anti-Connexin-43 may be used in immunoblotting, immunocytochemistry and immunohistochemistry (frozen and formalin-fixed, paraffin-embedded tissues). Polyclonal antibodies reacting specifically with Cx43 may be applied in diverse cellular and molecular approaches to the study of gap junctions and their properties.A minimum working dilution of 1:8,000 is determined by immunoblotting using a whole extract from mouse brain.A minimum working dilution of 1:400 is determined by indirect immunofluorescent staining of acetone-fixed cultured baby hamster kidney (BHK).A minimum working dilution of 1:2,000 is determined by indirect immunofluorescent staining of rat heart. (Negative on rat liver sections)A minimum working dilution of 1:2,000 is determined by indirect immunoperoxidase staining of trypsin-digested,formalin-fixed, paraffin-embedded human or animal tissue.
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    Structured Review

    Millipore polyclonal rabbit anti connexin 43 cx43
    Anti Connexin 43 antibody
    Anti Connexin 43 is developed in rabbit using a synthetic peptide conjugated to KLH with glutaraldehyde as immunogen The peptide corresponds to a C terminal segment of the cytoplasmic domain of human and rat connexin 43 amino acid residues with an N terminal added lysine Affinity isolated antigen specific antibody is obtained by immunospecific purification which removes essentially all rabbit serum proteins including immunoglobulins which do not specifically bind to connexin 43 Gap junctions are specialized cell membrane domains consisting of aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells electrical coupling synchronization of cellular physiological activities growth control and developmental regulation The gap junction channels allow intercellular exchange of ions nucleotides and small molecules between adjacent cells Unlike other membrane channels intercellular channels span two opposed plasma membranes and require the contribution of hemi channels called connexons from both participating cells These channels are permeable to molecules as large as 1 kDa and they have been reported in most mammalian cell types Two connexons interact in the extracellular space to form the complete intercellular channel Each connexon is composed of six similar or identical proteins which have been termed connexins Connexins Cx are a multi gene family of highly related proteins with molecular weights ranging from 26 to 70 kDa At least a dozen distinct connexin genes have been identified in mammals many expressed in a diverse tissue and cell specific pattern Two distinct lineages have been identified in mammals one termed class I or β group in which Cx26 Cx30 Cx31 Cx31 1 and Cx32 fall and the other termed class II or α group represented by Cx33 Cx37 Cx40 Cx43 and Cx46 Connexin 43 Cx 43 a 43kDa protein is a member of the connexin protein family that forms a gap junction It is expressed by a variety of cell types such as astrocytes cardiac and smooth muscle endothelium ependyma fibroblasts keratinocytes lens and corneal epithelium In addition the protein is also found in leptomeninges leucocytes Leydig cells macrophages myoepithelial cells of mammary gland osteocytes ovarian granulosa pancreatic β cells preimplantation blastocyst sertoli cells thyroid follicular cells and trophoblast giant cells
    https://www.bioz.com/result/polyclonal rabbit anti connexin 43 cx43/product/Millipore
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti connexin 43 cx43 - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "A Human Type 5 Adenovirus-Based Trypanosoma cruzi Therapeutic Vaccine Re-programs Immune Response and Reverses Chronic Cardiomyopathy"

    Article Title: A Human Type 5 Adenovirus-Based Trypanosoma cruzi Therapeutic Vaccine Re-programs Immune Response and Reverses Chronic Cardiomyopathy

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1004594

    rAdVax immunotherapy recovered the injured heart tissue of chronically T. cruzi -infected mice. ( A ) Chronically Colombian T. cruzi strain-infected mice were evaluated for heart injury markers pre-therapy (120 dpi) or primed-boosted with 2 × 10 8 plaque-forming units (PFU) of rAdCtrl or a mixture of 10 8 PFU of each adenovirus vaccine preparation (rAdASP2+rAdTS; rAdVax). Mortality was recorded until 230 dpi (110 days post-therapy; dpt), when the surviving mice were analyzed for heart injury markers. ( B ) Kaplan-Meier curve representing the percentages of surviving mice (14–20 mice/group in two independent experiments). ( C ) Relative spleen weight (mg of spleen/g of body) and quantitative immunohistochemical staining (IHS) data for T. cruzi parasitism (nests/100 microscopic fields) in the heart tissue of chronically infected mice (120 and 230 dpi, respectively, pre- and post-therapy). ( D ) IHS showing fibronectin (FN)-stained areas in representative cardiac tissue sections of noninfected (NI) controls and chronically T. cruzi -infected mice pre- (120 dpi) and post-therapy (230 dpi; 110 dpt) with rAdVax. ( E ) Quantification of the FN-stained area (%) and connexin 43 (Cx43)-containing gap junction distances detected using IHS staining of heart tissue sections of NI controls or T. cruzi -infected mice pre- and post-therapy with rAdVax. ( F ) Evaluation of CK-MB activity in the serum of NI controls and T. cruzi -infected mice pre- and post-therapy with rAdVax. The data are presented as the means ± SD. ** P
    Figure Legend Snippet: rAdVax immunotherapy recovered the injured heart tissue of chronically T. cruzi -infected mice. ( A ) Chronically Colombian T. cruzi strain-infected mice were evaluated for heart injury markers pre-therapy (120 dpi) or primed-boosted with 2 × 10 8 plaque-forming units (PFU) of rAdCtrl or a mixture of 10 8 PFU of each adenovirus vaccine preparation (rAdASP2+rAdTS; rAdVax). Mortality was recorded until 230 dpi (110 days post-therapy; dpt), when the surviving mice were analyzed for heart injury markers. ( B ) Kaplan-Meier curve representing the percentages of surviving mice (14–20 mice/group in two independent experiments). ( C ) Relative spleen weight (mg of spleen/g of body) and quantitative immunohistochemical staining (IHS) data for T. cruzi parasitism (nests/100 microscopic fields) in the heart tissue of chronically infected mice (120 and 230 dpi, respectively, pre- and post-therapy). ( D ) IHS showing fibronectin (FN)-stained areas in representative cardiac tissue sections of noninfected (NI) controls and chronically T. cruzi -infected mice pre- (120 dpi) and post-therapy (230 dpi; 110 dpt) with rAdVax. ( E ) Quantification of the FN-stained area (%) and connexin 43 (Cx43)-containing gap junction distances detected using IHS staining of heart tissue sections of NI controls or T. cruzi -infected mice pre- and post-therapy with rAdVax. ( F ) Evaluation of CK-MB activity in the serum of NI controls and T. cruzi -infected mice pre- and post-therapy with rAdVax. The data are presented as the means ± SD. ** P

    Techniques Used: Infection, Mouse Assay, Immunohistochemistry, Staining, Activity Assay

    Related Articles

    Incubation:

    Article Title: Functional Heterotypic Interactions Between Astrocyte and Oligodendrocyte Connexins
    Article Snippet: .. Cells were grown on glass bottom dishes (MatTek, Ashland, MA) or coverslips, fixed with 4% paraformaldehyde for 15 min at room temperature, blocked with 10% FBS in PBS containing 0.2% Tween-20, and incubated for 1 hour at room temperature in appropriate combinations of the following primary antibodies: rabbit anti-Cx43 (1:10,000 dilution; Sigma C-6219), mouse anti-Cx43 (1:100, Zymed 13-8300), rabbit anti-Cx47 (1:1000, ), rabbit anti-Cx32 (1:100; Zymed 34-5700), mouse anti-Cx32 (1:100, Zymed 13-8200), rabbit anti-Cx26 (1:50; Zymed 71-0500), mouse anti-Cx26 (1:100, Zymed 33-5800), rabbit anti-Cx30 (1:1000; Zymed 71-2200), or rabbit anti-GFAP(1:250 dilution; Dako, Carpinteria, CA). .. After incubation with primary antibodies, cells were washed and incubated with appropriate secondary antibodies (rhodamine-labeled goat anti-rabbit (1:200, Chemicon, Temecula, CA); Alexa568-labeled goat anti-mouse, (1:500, Invitrogen); Alex647-labeled goat anti-rabbit (1:1000, Invitrogen); Cy5-labeled donkey anti-goat (1:500, Jackson ImmunoResearch, West Grove, PA); or Cy3-labeled donkey anti-rabbit (1:500, Jackson ImmunoResearch).

    Article Title: HIV-Associated Cardiovascular Disease
    Article Snippet: .. Tissue sections were incubated in blocking solution for 2 hours at room temperature and then in diluted primary antibody (anti-pan Cx43: dilution 1:2000, Sigma C6219; Sigma-Aldrich, St. Louis, MO; or anti–N-cadherin: dilution 1:250, BD 61092; Becton Dickinson, Franklin Lakes, NJ) overnight at 4°C. .. Tissues were washed several times with phosphate-buffered saline at room temperature and incubated with the appropriate secondary antibody for at least 2 hours at room temperature, followed by another wash in phosphate-buffered saline.

    Article Title: A connexin43/YAP axis regulates astroglial-mesenchymal transition in hemoglobin induced astrocyte activation
    Article Snippet: .. The blots were blocked with 5% non-fat milk and incubated overnight at 4 °C with the appropriate primary antibodies: rabbit anti-Connexin 43 polyclonal antibody (1:1000, Cat# SAB4501175, Sigma, MO, USA), mouse anti-GFAP monoclonal antibody (1:3000, Cat# MAB3402, Millipore, MA, USA), mouse anti-Vimentin monoclonal antibody (1:3000, Cat# MAB3400, Millipore, MA, USA), rabbit anti-E-cadherin polyclonal antibody (1:1000, Cat# YT1454, Immunoway, TX, USA), rabbit anti-N-cadherin polyclonal antibody (1:1000, Cat# YT2988, Immunoway, TX, USA), rabbit anti-SLUG polyclonal antibody (1:1000, Cat# YM3371, Immunoway, TX, USA), rabbit anti-YAP monoclonal antibody (1:1000, Cat# 14074, Cell Signaling Technology, MA, USA), rabbit anti-Phospho-YAP(S127) monoclonal antibody (1:1000, Cat# 13008, Cell Signaling Technology, MA, USA), rabbit anti-histone-H3 monoclonal antibody (1:1000, Cat# 4499, Cell Signaling Technology, MA, USA), and rabbit anti-GAPDH monoclonal antibody (1:2000, Cat# 5174, Cell Signaling Technology, MA, USA). .. Subsequently, the membranes were washed thrice, then incubated with corresponding horseradish peroxidase conjugated secondary antibody for 1 h at room temperature.

    other:

    Article Title: Antiarrhythmic Effects of Dantrolene in Patients with Catecholaminergic Polymorphic Ventricular Tachycardia and Replication of the Responses Using iPSC Models
    Article Snippet: [ ] Single CMs were immunostained with anti-cardiac-troponin-T (1:1500, Abcam, Cambridge, MA, USA), anti-α-actinin (1:1500, Sigma) and anti-connexin-43 (1:1000, Sigma).

    Article Title: Vascular Endothelial-Cadherin Regulates Cytoskeletal Tension, Cell Spreading, and Focal Adhesions by Stimulating RhoA D⃞
    Article Snippet: The following reagents were purchased from the given suppliers: human fibronectin (Collaborative Research, Bedford, MA); bovine serum albumin (BSA; Serologicals, Norcross, GA); 2,3-butanedione 2-monoxime (BDM; Calbiochem, San Diego); Y-27632 (Calbiochem); anti-FAK clone 2A7 (Upstate Biotechnology, Lake Placid, NY); anti-FAK clone 77 (BD Transduction Laboratories, Lexington, KY); anti-vinculin clone hVin-1 (Sigma-Aldrich, St. Louis, MO); anti-talin clone 8D4 (Sigma-Aldrich); anti-phosphotyrosine clone 4G10 (Upstate Biotechnology); tetramethylrhodamine B isothiocyanate (TRITC)-conjugated phalloidin (Sigma-Aldrich); polyclonal rabbit anti-VE-cadherin (Alexis, Läufelfingen, Switzerland); anti-β-catenin clone E-5 (Santa Cruz Biotechnology, Santa Cruz, CA); anti-connexin 43 (Chemicon International, Temecula, CA); anti-platelet-endothelial cell adhesion molecule-1 (PECAM-1) (Santa Cruz Biotechnology); polyclonal rabbit anti-RhoA (Santa Cruz Biotechnology); and anti-p120-catenin clone 98 (BD Transduction Laboratories).

    Blocking Assay:

    Article Title: HIV-Associated Cardiovascular Disease
    Article Snippet: .. Tissue sections were incubated in blocking solution for 2 hours at room temperature and then in diluted primary antibody (anti-pan Cx43: dilution 1:2000, Sigma C6219; Sigma-Aldrich, St. Louis, MO; or anti–N-cadherin: dilution 1:250, BD 61092; Becton Dickinson, Franklin Lakes, NJ) overnight at 4°C. .. Tissues were washed several times with phosphate-buffered saline at room temperature and incubated with the appropriate secondary antibody for at least 2 hours at room temperature, followed by another wash in phosphate-buffered saline.

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  • 99
    Millipore rabbit anti connexin43
    Olfactory bulb astrocyte cultures respond to iodomelatonin. Top: control cultures treated with fresh medium. Bottom: cultures treated with 3 nM iodomelatonin in fresh medium. <t>Connexin43</t> immunoreactivity increased in the treated cultures compared to controls.
    Rabbit Anti Connexin43, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti connexin43/product/Millipore
    Average 99 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    rabbit anti connexin43 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Olfactory bulb astrocyte cultures respond to iodomelatonin. Top: control cultures treated with fresh medium. Bottom: cultures treated with 3 nM iodomelatonin in fresh medium. Connexin43 immunoreactivity increased in the treated cultures compared to controls.

    Journal: Neuroscience

    Article Title: Melatonin in the mammalian olfactory bulb

    doi: 10.1016/j.neuroscience.2013.12.033

    Figure Lengend Snippet: Olfactory bulb astrocyte cultures respond to iodomelatonin. Top: control cultures treated with fresh medium. Bottom: cultures treated with 3 nM iodomelatonin in fresh medium. Connexin43 immunoreactivity increased in the treated cultures compared to controls.

    Article Snippet: Primary antisera used in this study were the following: rabbit anti-MT1R (1:1000 in 1% milk/TBST; Abbiotec, 250761), rabbit anti-MT1R (1:1000 in 4% milk/TBST; 1:3000 in Ni-DAB IHC; Novus, NBP1-71113), goat anti-MT2R (1:500 in 4% milk/TBST; Santa Cruz, sc-13177), rabbit anti-MT2R (1:1000 in 4% milk/TBST, 1:10,000 in Ni-DAB IHC, 1:500 in fluorescent IHC (FIHC) using streptavidin and a biotinylated secondary antibody; Novus, NLS932), mouse antityrosine hydroxylase (1:30,000 in FIHC; Chemicon, MAB318), mouse anti-glutamic acid decarboxylase (GAD)-65 (1:10,000 in FIHC; Abcam, ab26113), mouse anti-GAD67 (1:10,000 in FIHC; Chemicon, MAB5406), goat anti-calretinin (1:30,000 in FIHC; Chemicon, AB1550), goat anti-parvalbumin (1:2000 in FIHC; Swant, PVG-214), rabbit monoclonal anti-β-III-tubulin (1:2000 in 4% milk/TBST; Cell Signaling Technology, 5568), and rabbit anti-connexin43 (1:1000 in immunocytochemistry; Millipore AB1728).

    Techniques:

    Expression of the gap-junction proteins connexin 26, connexin 32, and connexin 43 in H69 cells after treatment with NDMA and/or ESP, as determined by western blotting. H69 cells were incubated with either PBS (vehicle) or NDMA and/or ESP for 72 h, and the cells were collected for protein extraction. The blots of each groups were run under same experimental conditions and the images were cropped from different parts of the same gels, DATA represent the mean ± SE of five independent experiments. * P

    Journal: bioRxiv

    Article Title: Connexin 43 plays an important role in the transformation of human cholangiocytes upon stimulation with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine

    doi: 10.1101/418350

    Figure Lengend Snippet: Expression of the gap-junction proteins connexin 26, connexin 32, and connexin 43 in H69 cells after treatment with NDMA and/or ESP, as determined by western blotting. H69 cells were incubated with either PBS (vehicle) or NDMA and/or ESP for 72 h, and the cells were collected for protein extraction. The blots of each groups were run under same experimental conditions and the images were cropped from different parts of the same gels, DATA represent the mean ± SE of five independent experiments. * P

    Article Snippet: The membranes were then probed with antibodies against E2F1, Ki-67, Ck19, Cox-2, connexin 43, connexin 32, connexin 26, and calnexin.

    Techniques: Expressing, Western Blot, Incubation, Protein Extraction

    Concentration of intracellular connexin 26, connexin 32, and connexin 43 in human cholangiocytes (H69 cells). After treatment with NDMA and ESP for 72 h, the concentration of intracellular connexin 26 in H69 cells was measured by laser scanning microscopy (×4,000). Scale bar = 25 µm. Data represent the mean ± SE of five independent experiments. * P

    Journal: bioRxiv

    Article Title: Connexin 43 plays an important role in the transformation of human cholangiocytes upon stimulation with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine

    doi: 10.1101/418350

    Figure Lengend Snippet: Concentration of intracellular connexin 26, connexin 32, and connexin 43 in human cholangiocytes (H69 cells). After treatment with NDMA and ESP for 72 h, the concentration of intracellular connexin 26 in H69 cells was measured by laser scanning microscopy (×4,000). Scale bar = 25 µm. Data represent the mean ± SE of five independent experiments. * P

    Article Snippet: The membranes were then probed with antibodies against E2F1, Ki-67, Ck19, Cox-2, connexin 43, connexin 32, connexin 26, and calnexin.

    Techniques: Concentration Assay, Laser-Scanning Microscopy

    Effect of connexin 43 silencing in H69 cells. A. Reduced cell proliferation upon NDMA and ESP treatment in H69 cells by connexin 43 silencing. B. Uptake of Cx43 siRNA reduces Cx43 expression, as confirmed by real-time PCR. Cx43 expression was remarkably reduced in H69 cells transfected with Cx43 -specific siRNA. C. Ratio of Cx26/GAPDH in H69 cells transfected with Cx43 -specific siRNA. Cx26 expression was remarkably reduced in H69 cells transfected with Cx43 -specific siRNA. D. The ratio of Cox-2/GAPDH in H69 cells transfected with Cx43 -specific siRNA. Cox-2 expression was remarkably reduced in H69 cells transfected with Cx43 -specific siRNA. Data represent the mean ± SE of five independent experiments. * P

    Journal: bioRxiv

    Article Title: Connexin 43 plays an important role in the transformation of human cholangiocytes upon stimulation with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine

    doi: 10.1101/418350

    Figure Lengend Snippet: Effect of connexin 43 silencing in H69 cells. A. Reduced cell proliferation upon NDMA and ESP treatment in H69 cells by connexin 43 silencing. B. Uptake of Cx43 siRNA reduces Cx43 expression, as confirmed by real-time PCR. Cx43 expression was remarkably reduced in H69 cells transfected with Cx43 -specific siRNA. C. Ratio of Cx26/GAPDH in H69 cells transfected with Cx43 -specific siRNA. Cx26 expression was remarkably reduced in H69 cells transfected with Cx43 -specific siRNA. D. The ratio of Cox-2/GAPDH in H69 cells transfected with Cx43 -specific siRNA. Cox-2 expression was remarkably reduced in H69 cells transfected with Cx43 -specific siRNA. Data represent the mean ± SE of five independent experiments. * P

    Article Snippet: The membranes were then probed with antibodies against E2F1, Ki-67, Ck19, Cox-2, connexin 43, connexin 32, connexin 26, and calnexin.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection

    Expression of Connexin 43 protein in testes of adult wild-type (+/0) and NONO-deficient ( gt/ 0) mice. (A) Sections stained for Connexin 43 expression (red) and counterstained with DAPI as a nuclear marker (blue). Scale bar: 30 μm. Images were collected two mice of each genotype; one representative image is shown. (B) Immunoblot of protein extract from testes of wild-type and NONO-deficient mice probed for NONO and Connexin 43 (Cx43) expression. Blots were probed for β-actin as a loading control. Samples were analyzed for three mice of each genotype; a representative pair is shown. (C) Plot showing quantification of Cx43 levels, normalized to β-actin and expressed relative to non-irradiated Nono +/0 control (marked by dotted line).

    Journal: DNA repair

    Article Title: Cell-type specific role of the RNA-binding protein, NONO, in the DNA double-strand break response in the mouse testes

    doi: 10.1016/j.dnarep.2017.02.002

    Figure Lengend Snippet: Expression of Connexin 43 protein in testes of adult wild-type (+/0) and NONO-deficient ( gt/ 0) mice. (A) Sections stained for Connexin 43 expression (red) and counterstained with DAPI as a nuclear marker (blue). Scale bar: 30 μm. Images were collected two mice of each genotype; one representative image is shown. (B) Immunoblot of protein extract from testes of wild-type and NONO-deficient mice probed for NONO and Connexin 43 (Cx43) expression. Blots were probed for β-actin as a loading control. Samples were analyzed for three mice of each genotype; a representative pair is shown. (C) Plot showing quantification of Cx43 levels, normalized to β-actin and expressed relative to non-irradiated Nono +/0 control (marked by dotted line).

    Article Snippet: Primary antibodies were: mouse anti-SFPQ (Sigma-Aldrich, WH0006421M2, 1:500), goat anti-NONO (GeneTex, GTX89315, 1:50), rabbit anti-PSPC1 (Bethyl Laboratories, A303-205A, 1:500), rabbit anti-SOX9 (EMD Millipore, AB5535, 1:1500), rabbit anti-Connexin 43 (EMD Millipore, AB11370, 1:1000) and rabbit anti-53BP1 (Novus Biologicals, NB100-904, 1:1000).

    Techniques: Expressing, Mouse Assay, Staining, Marker, Irradiation