mouse monoclonal anti cardiac troponin t  (Abcam)

 
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    Anti Cardiac Troponin T antibody 1C11
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    Structured Review

    Abcam mouse monoclonal anti cardiac troponin t
    Relative gene expression level of differentiating H9c2 cardiomyoblasts into cardiomyocytes on gelatin, fibronectin, and FDM at DM7 compared to the one at GM3. Cardiomyogenic marker genes are actin alpha cardiac 1, myosin light chain 2, and cardiac muscle <t>troponin</t> T (Actc1, Myl2, and Tnnt, respectively). Statistical significance (* p

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    Images

    1) Product Images from "Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment"

    Article Title: Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment

    Journal: Tissue Engineering. Part A

    doi: 10.1089/ten.tea.2014.0591

    Relative gene expression level of differentiating H9c2 cardiomyoblasts into cardiomyocytes on gelatin, fibronectin, and FDM at DM7 compared to the one at GM3. Cardiomyogenic marker genes are actin alpha cardiac 1, myosin light chain 2, and cardiac muscle troponin T (Actc1, Myl2, and Tnnt, respectively). Statistical significance (* p
    Figure Legend Snippet: Relative gene expression level of differentiating H9c2 cardiomyoblasts into cardiomyocytes on gelatin, fibronectin, and FDM at DM7 compared to the one at GM3. Cardiomyogenic marker genes are actin alpha cardiac 1, myosin light chain 2, and cardiac muscle troponin T (Actc1, Myl2, and Tnnt, respectively). Statistical significance (* p

    Techniques Used: Expressing, Marker

    2) Product Images from "Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling"

    Article Title: Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling

    Journal: BMC Developmental Biology

    doi: 10.1186/s12861-016-0112-2

    The proliferative capacity of the induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs). a , double immunostainings for Ki67 (a marker for cycling cells, green) and cTnT (a marker for CM, red), nuclei in the same field were stained with DAPI (blue). b , the percentage of proliferating iPS-CMs in control (7.9 ± 1.1 %), co-culture (10.4 ± 1.2 %), FAK siRNA (4.1 ± 0.5 %), and SP600125 (1.8 ± 0.2 %). Scale bar =100 μm, *: P
    Figure Legend Snippet: The proliferative capacity of the induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs). a , double immunostainings for Ki67 (a marker for cycling cells, green) and cTnT (a marker for CM, red), nuclei in the same field were stained with DAPI (blue). b , the percentage of proliferating iPS-CMs in control (7.9 ± 1.1 %), co-culture (10.4 ± 1.2 %), FAK siRNA (4.1 ± 0.5 %), and SP600125 (1.8 ± 0.2 %). Scale bar =100 μm, *: P

    Techniques Used: Derivative Assay, Marker, Staining, Co-Culture Assay

    3) Product Images from "Arrhythmogenic properties of dismantling cadherin-mediated adhesion in murine hearts"

    Article Title: Arrhythmogenic properties of dismantling cadherin-mediated adhesion in murine hearts

    Journal: Journal of Biomedical Research

    doi: 10.1016/S1674-8301(10)60041-3

    Effects of rmAPN on the phosphorylation of Cx43. A: Immunoblot of total Cx43 in heart tissues from both rmAPN- and saline-treated mice. B: Immunoblot of p-Cx43 in the heart tissue of both groups. C: The relative density of total Cx43 and p-Cx43 normalized to GAPDH (* P
    Figure Legend Snippet: Effects of rmAPN on the phosphorylation of Cx43. A: Immunoblot of total Cx43 in heart tissues from both rmAPN- and saline-treated mice. B: Immunoblot of p-Cx43 in the heart tissue of both groups. C: The relative density of total Cx43 and p-Cx43 normalized to GAPDH (* P

    Techniques Used: Mouse Assay

    Redistribution of Cx43 in rmAPN-treated mice. A-F: Representative laser confocal images of left ventricular myocardia from rmAPN- and saline-treated mice immunostained with anti-Cx43 and anti-N-cad antibodies. A strong Cx43 signal (red) at the IDs throughout rmAPN-(D-F) and saline-treated heart tissues(A-C) and overlay of signals show the colocalization of the proteins. Arrows show the Cx43 signal redistribution onto the scarcolemma in rmAPN-treated hearts. Bar=20 µm for all the panels. G: Comparison of the signals of N-cad and Cx43 in tip-to-tip fashion in the heart tissue from both groups (* P
    Figure Legend Snippet: Redistribution of Cx43 in rmAPN-treated mice. A-F: Representative laser confocal images of left ventricular myocardia from rmAPN- and saline-treated mice immunostained with anti-Cx43 and anti-N-cad antibodies. A strong Cx43 signal (red) at the IDs throughout rmAPN-(D-F) and saline-treated heart tissues(A-C) and overlay of signals show the colocalization of the proteins. Arrows show the Cx43 signal redistribution onto the scarcolemma in rmAPN-treated hearts. Bar=20 µm for all the panels. G: Comparison of the signals of N-cad and Cx43 in tip-to-tip fashion in the heart tissue from both groups (* P

    Techniques Used: Mouse Assay

    Influence of various concentrations of rmAPN on cell-cell adhesion and Cx43 distribution in vitro . Cultured neonatal rat cardiomyocytes were incubated with or without rmAPN and were double-immunostained with N-cad (green, A-E) and Cx43 (red, F-J). A and F: Normal cell-to-cell connections. N-cad and Cx43 signals concentrated in the connections between adjacent cells. B and G: Cells incubated with rmAPN (10 ng/µL). C and H: Cells incubated with rmAPN (20 ng/µL) display Cx43 signal pulling away from the cell-cell junctions and distributed into the cytoplasm (arrows). D and I: Cells incubated with 30 ng/µl rmAPN showing decreased N-cad signals, broken cell-cell connections (arrowheads), and diffused distribution of Cx43 (arrows) into the plasmalemma. E and J: Cells incubated with 40 ng/µL rmAPN. Scale bar = 10 µm, for all the panels.
    Figure Legend Snippet: Influence of various concentrations of rmAPN on cell-cell adhesion and Cx43 distribution in vitro . Cultured neonatal rat cardiomyocytes were incubated with or without rmAPN and were double-immunostained with N-cad (green, A-E) and Cx43 (red, F-J). A and F: Normal cell-to-cell connections. N-cad and Cx43 signals concentrated in the connections between adjacent cells. B and G: Cells incubated with rmAPN (10 ng/µL). C and H: Cells incubated with rmAPN (20 ng/µL) display Cx43 signal pulling away from the cell-cell junctions and distributed into the cytoplasm (arrows). D and I: Cells incubated with 30 ng/µl rmAPN showing decreased N-cad signals, broken cell-cell connections (arrowheads), and diffused distribution of Cx43 (arrows) into the plasmalemma. E and J: Cells incubated with 40 ng/µL rmAPN. Scale bar = 10 µm, for all the panels.

    Techniques Used: In Vitro, Cell Culture, Incubation

    4) Product Images from "Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling"

    Article Title: Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling

    Journal: BMC Developmental Biology

    doi: 10.1186/s12861-016-0112-2

    Expression of cardiac specific proteins in induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) on day 12. a , double immunostaining of cells by antibodies against cTnT (red) and CX43 (green). b , double immunofluorescent staining for cTnT (red) and MLC-2 V (green), c , double immunofluorescent staining for cTnT (red) and α-actinin (green). Nuclei in the same field were stained with DAPI (blue). Merged figures were made by FV10-ASW Systems. d , growth curves of cells in co-culture and control analyzed by a MTT assay. The growth of the cells in co-culture was significantly better than that in control after day 6. Scale bar =20 μm,*: P
    Figure Legend Snippet: Expression of cardiac specific proteins in induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) on day 12. a , double immunostaining of cells by antibodies against cTnT (red) and CX43 (green). b , double immunofluorescent staining for cTnT (red) and MLC-2 V (green), c , double immunofluorescent staining for cTnT (red) and α-actinin (green). Nuclei in the same field were stained with DAPI (blue). Merged figures were made by FV10-ASW Systems. d , growth curves of cells in co-culture and control analyzed by a MTT assay. The growth of the cells in co-culture was significantly better than that in control after day 6. Scale bar =20 μm,*: P

    Techniques Used: Expressing, Derivative Assay, Double Immunostaining, Staining, Co-Culture Assay, MTT Assay

    5) Product Images from "Calcium signals in the nucleus accumbens: Activation of astrocytes by ATP and succinate"

    Article Title: Calcium signals in the nucleus accumbens: Activation of astrocytes by ATP and succinate

    Journal: BMC Neuroscience

    doi: 10.1186/1471-2202-12-96

    Co-localization of ATP-evoked Ca 2+ transients with Cx43 immunostaining in the NAc slice . Representative images of Ca 2+ signalling in response to the long ATP puff (green, left) followed by post-calcium ( cf . Methods section) Cx43 immunostaining (red, middle) demonstrated their co-localization (yellow spots marked with white arrows, right). Scale bar: 50 μm.
    Figure Legend Snippet: Co-localization of ATP-evoked Ca 2+ transients with Cx43 immunostaining in the NAc slice . Representative images of Ca 2+ signalling in response to the long ATP puff (green, left) followed by post-calcium ( cf . Methods section) Cx43 immunostaining (red, middle) demonstrated their co-localization (yellow spots marked with white arrows, right). Scale bar: 50 μm.

    Techniques Used: Immunostaining

    Cx43 is present in GFAP-containing astrocytes in the NAc . A : Cx43 immunoreactivity in a coronal section of a PFA-fixed rat brain shows relatively high intensity in the NAc, as compared to adjacent brain regions. Abbreviations: ac - anterior commissure, CP caudate-putamen, LS - lateral septal nucleus, LV - lateral ventricle, NAc - nucleus accumbens, VP - ventral pallidum. Scale bar: 1 mm. B: Comparison of the appearance of Cx43 (red) and GFAP (green) proteins in the NAc. Low magnification (scale bar: 400 μm) confocal photomicrograph of a double labelled section of a PFA-fixed rat brain. Co-localization (yellow) of Cx43 (red) and GFAP (green) immunoreactivities in the NAc suggests that Cx43 is present in GFAP-containing astrocytes in this brain region. Label
    Figure Legend Snippet: Cx43 is present in GFAP-containing astrocytes in the NAc . A : Cx43 immunoreactivity in a coronal section of a PFA-fixed rat brain shows relatively high intensity in the NAc, as compared to adjacent brain regions. Abbreviations: ac - anterior commissure, CP caudate-putamen, LS - lateral septal nucleus, LV - lateral ventricle, NAc - nucleus accumbens, VP - ventral pallidum. Scale bar: 1 mm. B: Comparison of the appearance of Cx43 (red) and GFAP (green) proteins in the NAc. Low magnification (scale bar: 400 μm) confocal photomicrograph of a double labelled section of a PFA-fixed rat brain. Co-localization (yellow) of Cx43 (red) and GFAP (green) immunoreactivities in the NAc suggests that Cx43 is present in GFAP-containing astrocytes in this brain region. Label "x" indicates the site of ATP application. C: High magnification (scale bar: 20 μm) confocal photomicrograph of a double labelled section of a PFA-fixed rat brain. Yellow colour indicates co-localizations of Cx43 (red) and GFAP (green) immunoreactivities in the PFA-fixed NAc.

    Techniques Used:

    6) Product Images from "Characteristics and Cardiomyogenic Potential of Rat Fetal Cardiac Progenitor Cells at Different Developmental Stage"

    Article Title: Characteristics and Cardiomyogenic Potential of Rat Fetal Cardiac Progenitor Cells at Different Developmental Stage

    Journal: Tissue Engineering and Regenerative Medicine

    doi: 10.1007/s13770-016-0016-z

    Oct4 and Sox2 expression in rat embryos at E12 and E15 from gestation. Embryos were collected from pregnant SD rat at E12 (chamber formation stage) and E15 (septation stage) and protein localization of Oct4 and Sox2 was analyzed using immunohistochemistry after sagittal section. A Oct4 protein expression in embryo of E12, B Oct4 protein expression in embryo of E15, C Sox2 protein expression in embryo of E12, D Sox2 protein expression in embryo of E15, E Cardiac development stage. The septation of the atria and ventricles, and remodeling of the outflow tract to form the aorta and the pulmonary artery (High and Epstein 2008). Oct4 was expressed in brain, liver, heart, and skin of E12 and E15 embryos. Sox2 was intensively expressed in brain of E12 and E15 embryos
    Figure Legend Snippet: Oct4 and Sox2 expression in rat embryos at E12 and E15 from gestation. Embryos were collected from pregnant SD rat at E12 (chamber formation stage) and E15 (septation stage) and protein localization of Oct4 and Sox2 was analyzed using immunohistochemistry after sagittal section. A Oct4 protein expression in embryo of E12, B Oct4 protein expression in embryo of E15, C Sox2 protein expression in embryo of E12, D Sox2 protein expression in embryo of E15, E Cardiac development stage. The septation of the atria and ventricles, and remodeling of the outflow tract to form the aorta and the pulmonary artery (High and Epstein 2008). Oct4 was expressed in brain, liver, heart, and skin of E12 and E15 embryos. Sox2 was intensively expressed in brain of E12 and E15 embryos

    Techniques Used: Expressing, Immunohistochemistry

    Expression of embryonic stem cells (ESCs) markers in rFCPCs of E12 and E15. A rFCPCs expressed pluripotent markers, Oct4 and Nanog but not Sox2 at passages 1 (P1) and 5 (P5) in RT-PCR analysis. B Oct4 and Sox2 was expressed in E12 and E15 rFCPCs passage 3 in immune-staining. Both cells of E12 and E15 expressed proteins Oct4. Scale bar 100 mm
    Figure Legend Snippet: Expression of embryonic stem cells (ESCs) markers in rFCPCs of E12 and E15. A rFCPCs expressed pluripotent markers, Oct4 and Nanog but not Sox2 at passages 1 (P1) and 5 (P5) in RT-PCR analysis. B Oct4 and Sox2 was expressed in E12 and E15 rFCPCs passage 3 in immune-staining. Both cells of E12 and E15 expressed proteins Oct4. Scale bar 100 mm

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining

    7) Product Images from "Characteristics and Cardiomyogenic Potential of Rat Fetal Cardiac Progenitor Cells at Different Developmental Stage"

    Article Title: Characteristics and Cardiomyogenic Potential of Rat Fetal Cardiac Progenitor Cells at Different Developmental Stage

    Journal: Tissue Engineering and Regenerative Medicine

    doi: 10.1007/s13770-016-0016-z

    Oct4 and Sox2 expression in rat embryos at E12 and E15 from gestation. Embryos were collected from pregnant SD rat at E12 (chamber formation stage) and E15 (septation stage) and protein localization of Oct4 and Sox2 was analyzed using immunohistochemistry after sagittal section. A Oct4 protein expression in embryo of E12, B Oct4 protein expression in embryo of E15, C Sox2 protein expression in embryo of E12, D Sox2 protein expression in embryo of E15, E Cardiac development stage. The septation of the atria and ventricles, and remodeling of the outflow tract to form the aorta and the pulmonary artery (High and Epstein 2008). Oct4 was expressed in brain, liver, heart, and skin of E12 and E15 embryos. Sox2 was intensively expressed in brain of E12 and E15 embryos
    Figure Legend Snippet: Oct4 and Sox2 expression in rat embryos at E12 and E15 from gestation. Embryos were collected from pregnant SD rat at E12 (chamber formation stage) and E15 (septation stage) and protein localization of Oct4 and Sox2 was analyzed using immunohistochemistry after sagittal section. A Oct4 protein expression in embryo of E12, B Oct4 protein expression in embryo of E15, C Sox2 protein expression in embryo of E12, D Sox2 protein expression in embryo of E15, E Cardiac development stage. The septation of the atria and ventricles, and remodeling of the outflow tract to form the aorta and the pulmonary artery (High and Epstein 2008). Oct4 was expressed in brain, liver, heart, and skin of E12 and E15 embryos. Sox2 was intensively expressed in brain of E12 and E15 embryos

    Techniques Used: Expressing, Immunohistochemistry

    Expression of embryonic stem cells (ESCs) markers in rFCPCs of E12 and E15. A rFCPCs expressed pluripotent markers, Oct4 and Nanog but not Sox2 at passages 1 (P1) and 5 (P5) in RT-PCR analysis. B Oct4 and Sox2 was expressed in E12 and E15 rFCPCs passage 3 in immune-staining. Both cells of E12 and E15 expressed proteins Oct4. Scale bar 100 mm
    Figure Legend Snippet: Expression of embryonic stem cells (ESCs) markers in rFCPCs of E12 and E15. A rFCPCs expressed pluripotent markers, Oct4 and Nanog but not Sox2 at passages 1 (P1) and 5 (P5) in RT-PCR analysis. B Oct4 and Sox2 was expressed in E12 and E15 rFCPCs passage 3 in immune-staining. Both cells of E12 and E15 expressed proteins Oct4. Scale bar 100 mm

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining

    8) Product Images from "Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment"

    Article Title: Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment

    Journal: Tissue Engineering. Part A

    doi: 10.1089/ten.tea.2014.0591

    H9c2 cardiomyoblast differentiation into cardiomyocytes on FDM and X-FDM at DM7. (A) IFS of α-actinin and (B) cTnT (both shown in green ), along with nuclei stained with DAPI ( blue ). Insets show the cells before the induction of differentiation at GM3. (C) Quantitative analysis of nuclei positive for α-actinin and cTnT based on IFS image analysis using ImageJ. Data collected from five random IFS images. Statistical significance (** p
    Figure Legend Snippet: H9c2 cardiomyoblast differentiation into cardiomyocytes on FDM and X-FDM at DM7. (A) IFS of α-actinin and (B) cTnT (both shown in green ), along with nuclei stained with DAPI ( blue ). Insets show the cells before the induction of differentiation at GM3. (C) Quantitative analysis of nuclei positive for α-actinin and cTnT based on IFS image analysis using ImageJ. Data collected from five random IFS images. Statistical significance (** p

    Techniques Used: Staining

    H9c2 cardiomyoblast differentiation into cardiomyocytes on gelatin, fibronectin, and FDM at DM7. (A) IFS of α-actinin and cTnT (both shown in green ) and nuclei stained with DAPI ( blue ). Insets are the cells before the induction of differentiation at GM3. Scale bars in all images (including insets ) are 100 μm. (B) IFS of primary cardiomyocytes (CM) as a positive control stained with α-actinin and cTnT, respectively, and nuclei stained with DAPI ( blue ). Scale bar=50 μm. (C) Quantitative analysis of nuclei positive for α-actinin and cTnT at DM7 based on IFS image analysis using ImageJ. Data collected from five random IFS images. Statistical significance (* p
    Figure Legend Snippet: H9c2 cardiomyoblast differentiation into cardiomyocytes on gelatin, fibronectin, and FDM at DM7. (A) IFS of α-actinin and cTnT (both shown in green ) and nuclei stained with DAPI ( blue ). Insets are the cells before the induction of differentiation at GM3. Scale bars in all images (including insets ) are 100 μm. (B) IFS of primary cardiomyocytes (CM) as a positive control stained with α-actinin and cTnT, respectively, and nuclei stained with DAPI ( blue ). Scale bar=50 μm. (C) Quantitative analysis of nuclei positive for α-actinin and cTnT at DM7 based on IFS image analysis using ImageJ. Data collected from five random IFS images. Statistical significance (* p

    Techniques Used: Staining, Positive Control

    9) Product Images from "Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells"

    Article Title: Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2017.3259

    Western blotting and reverse transcription-quantitative polymerase chain reaction analysis of target protein and mRNA expression post-transduction. (A) pHBAd-MCMV-GFP-TBX18-transduced BMSCs displayed exogenous TBX18 expression, as determined by western blotting. (B) Protein expression levels of TBX18 were verified in differentiated cells by western blotting. (C) Alterations in the relative mRNA expression levels of TBX18 were compared between the T group and the G and B groups. (D) Quantitative analysis of the mRNA expression levels of α-actin, HCN4 and CX43. (E) Western blotting detected increased α-actin, cTnI and HCN4 protein expression, and reduced CX43 protein expression, in the T group compared with in the G and B groups. ※ P
    Figure Legend Snippet: Western blotting and reverse transcription-quantitative polymerase chain reaction analysis of target protein and mRNA expression post-transduction. (A) pHBAd-MCMV-GFP-TBX18-transduced BMSCs displayed exogenous TBX18 expression, as determined by western blotting. (B) Protein expression levels of TBX18 were verified in differentiated cells by western blotting. (C) Alterations in the relative mRNA expression levels of TBX18 were compared between the T group and the G and B groups. (D) Quantitative analysis of the mRNA expression levels of α-actin, HCN4 and CX43. (E) Western blotting detected increased α-actin, cTnI and HCN4 protein expression, and reduced CX43 protein expression, in the T group compared with in the G and B groups. ※ P

    Techniques Used: Western Blot, Real-time Polymerase Chain Reaction, Expressing, Transduction

    10) Product Images from "Connexin 43 acts as a cytoprotective mediator of signal transduction by stimulating mitochondrial KATP channels in mouse cardiomyocytes"

    Article Title: Connexin 43 acts as a cytoprotective mediator of signal transduction by stimulating mitochondrial KATP channels in mouse cardiomyocytes

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI40927

    Cx43 is present in isolated subsarcolemmal mitoplasts. Mitochondria were prepared by differential centrifugation as described in Methods. An equal amount of protein (30 μg) from distinct isolation fractions — i.e., crude homogenate of isolated myocytes (TP), first pellet with cell debris (P1, discarded), second pellet containing mitochondria (P2), second supernatant (S2, discarded), intact mitochondria (P3), mitoplasts (Mitopl) — was loaded in each lane, and proteins were analyzed by immunoblotting. Immunoreactivity to the following proteins served as markers for cellular compartments: GAPDH (cytosolic marker), Na + /K + -ATPase (plasma membrane marker), SERCA2A (endoplasmic reticulum marker), VDAC (outer mitochondrial membrane marker), UCP2 (inner mitochondrial membrane marker).
    Figure Legend Snippet: Cx43 is present in isolated subsarcolemmal mitoplasts. Mitochondria were prepared by differential centrifugation as described in Methods. An equal amount of protein (30 μg) from distinct isolation fractions — i.e., crude homogenate of isolated myocytes (TP), first pellet with cell debris (P1, discarded), second pellet containing mitochondria (P2), second supernatant (S2, discarded), intact mitochondria (P3), mitoplasts (Mitopl) — was loaded in each lane, and proteins were analyzed by immunoblotting. Immunoreactivity to the following proteins served as markers for cellular compartments: GAPDH (cytosolic marker), Na + /K + -ATPase (plasma membrane marker), SERCA2A (endoplasmic reticulum marker), VDAC (outer mitochondrial membrane marker), UCP2 (inner mitochondrial membrane marker).

    Techniques Used: Isolation, Centrifugation, Marker

    IP increases mitochondrial Cx43 content in Cx43 +/– mice and rescues diazoxide sensitivity of mitoK ATP channels ( A – E ). ( A ) Western blots of Cx43 and MN-SOD in mitochondria from the AW subjected to IP and PW (no IP) of the same Cx43 +/– mouse (IP-induced Cx43 increase normalized to MN-SOD, 4.0 ± 0.9, n = 3). Mitochondrial proteins from wild-type left ventricle (WT LV): positive control; Na + /K + -ATPase: exclusion of contamination with sarcolemmal proteins. ( B ) The diazoxide effect on mitoK ATP activity (at –60 mV) in Cx43 +/– mice was enhanced by IP (right) versus control (left). ( C and E ) Mean values of open probability (Po,total) ( C ) and mean open time ( E ) in PW (black) and AW (IP; white) of Cx43 +/– mice in the absence and presence of diazoxide, as indicated; n values are shown in parentheses. ( D ) Single-channel amplitude as a function of test potentials. Slope conductances were similar in PW and AW (subjected to IP) of Cx43 +/– mice and were unaffected by diazoxide.* P
    Figure Legend Snippet: IP increases mitochondrial Cx43 content in Cx43 +/– mice and rescues diazoxide sensitivity of mitoK ATP channels ( A – E ). ( A ) Western blots of Cx43 and MN-SOD in mitochondria from the AW subjected to IP and PW (no IP) of the same Cx43 +/– mouse (IP-induced Cx43 increase normalized to MN-SOD, 4.0 ± 0.9, n = 3). Mitochondrial proteins from wild-type left ventricle (WT LV): positive control; Na + /K + -ATPase: exclusion of contamination with sarcolemmal proteins. ( B ) The diazoxide effect on mitoK ATP activity (at –60 mV) in Cx43 +/– mice was enhanced by IP (right) versus control (left). ( C and E ) Mean values of open probability (Po,total) ( C ) and mean open time ( E ) in PW (black) and AW (IP; white) of Cx43 +/– mice in the absence and presence of diazoxide, as indicated; n values are shown in parentheses. ( D ) Single-channel amplitude as a function of test potentials. Slope conductances were similar in PW and AW (subjected to IP) of Cx43 +/– mice and were unaffected by diazoxide.* P

    Techniques Used: Mouse Assay, Western Blot, Positive Control, Activity Assay

    11) Product Images from "Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling"

    Article Title: Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling

    Journal: BMC Developmental Biology

    doi: 10.1186/s12861-016-0112-2

    The effects of focal adhesion kinase (FAK) siRNA or SP600125 on myocardial differentiation.  a , the expression of Mef2c, cTnT, MLC-2 V and BCL-2 after pretreation of FAK siRNA or SP600125 was examined by RT-PCR.  b ,  c ,  d ,  e , the quantitative analysis on Mef2c, cTnT, MLC-2V, and Bcl-2 expressions after pretreation of FAK siRNA or SP600125 by a real-time PCR. *:  P
    Figure Legend Snippet: The effects of focal adhesion kinase (FAK) siRNA or SP600125 on myocardial differentiation. a , the expression of Mef2c, cTnT, MLC-2 V and BCL-2 after pretreation of FAK siRNA or SP600125 was examined by RT-PCR. b , c , d , e , the quantitative analysis on Mef2c, cTnT, MLC-2V, and Bcl-2 expressions after pretreation of FAK siRNA or SP600125 by a real-time PCR. *: P

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Expression of cardiac specific proteins in induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) on day 12.  a , double immunostaining of cells by antibodies against cTnT (red) and CX43 (green).  b , double immunofluorescent staining for cTnT (red) and MLC-2 V (green),  c , double immunofluorescent staining for cTnT (red) and α-actinin (green). Nuclei in the same field were stained with DAPI (blue). Merged figures were made by FV10-ASW Systems.  d , growth curves of cells in co-culture and control analyzed by a MTT assay. The growth of the cells in co-culture was significantly better than that in control after day 6. Scale bar =20 μm,*:  P
    Figure Legend Snippet: Expression of cardiac specific proteins in induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) on day 12. a , double immunostaining of cells by antibodies against cTnT (red) and CX43 (green). b , double immunofluorescent staining for cTnT (red) and MLC-2 V (green), c , double immunofluorescent staining for cTnT (red) and α-actinin (green). Nuclei in the same field were stained with DAPI (blue). Merged figures were made by FV10-ASW Systems. d , growth curves of cells in co-culture and control analyzed by a MTT assay. The growth of the cells in co-culture was significantly better than that in control after day 6. Scale bar =20 μm,*: P

    Techniques Used: Expressing, Derivative Assay, Double Immunostaining, Staining, Co-Culture Assay, MTT Assay

    12) Product Images from "Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells"

    Article Title: Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2017.3259

    Fluorescence images of BMSCs post-transduction (magnification, ×200). (A) BMSCs were transduced with pHBAd-MCMV-GFP-TBX18, and HCN4 and GFP expression were detected. Nuclei were stained blue with DAPI. (B) BMSCs transduced with the pHBAd-MCMV-GFP control vector expressed GFP but not HCN4. BMSCs, bone mesenchymal stem cells; DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein; HCN4, hyperpolarization-activated cyclic nucleotide-gated channel 4; TBX18, T-box protein 18.
    Figure Legend Snippet: Fluorescence images of BMSCs post-transduction (magnification, ×200). (A) BMSCs were transduced with pHBAd-MCMV-GFP-TBX18, and HCN4 and GFP expression were detected. Nuclei were stained blue with DAPI. (B) BMSCs transduced with the pHBAd-MCMV-GFP control vector expressed GFP but not HCN4. BMSCs, bone mesenchymal stem cells; DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein; HCN4, hyperpolarization-activated cyclic nucleotide-gated channel 4; TBX18, T-box protein 18.

    Techniques Used: Fluorescence, Transduction, Expressing, Staining, Plasmid Preparation

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    Staining:

    Article Title: Superparamagnetic Iron Oxide Nanoparticles Function as a Long-Term, Multi-Modal Imaging Label for Non-Invasive Tracking of Implanted Progenitor Cells
    Article Snippet: .. Slides were then used for either routine histological staining (Masson's trichrome or Prussian blue with pararosaniline) or immunohistochemical staining with the following antibodies: anti-sarcomeric α-actinin (ACTN) (Abcam ab68167), anti-macrophage (MAC387) (Abcam ab22506), or anti-cardiac troponin T antibody (cTnT) (Abcam ab8295). .. Some slides were fluorescently labeled with Alexa Fluor 488 phalloidin (Life Technologies).

    Immunohistochemistry:

    Article Title: Superparamagnetic Iron Oxide Nanoparticles Function as a Long-Term, Multi-Modal Imaging Label for Non-Invasive Tracking of Implanted Progenitor Cells
    Article Snippet: .. Slides were then used for either routine histological staining (Masson's trichrome or Prussian blue with pararosaniline) or immunohistochemical staining with the following antibodies: anti-sarcomeric α-actinin (ACTN) (Abcam ab68167), anti-macrophage (MAC387) (Abcam ab22506), or anti-cardiac troponin T antibody (cTnT) (Abcam ab8295). .. Some slides were fluorescently labeled with Alexa Fluor 488 phalloidin (Life Technologies).

    Whole Genome Amplification:

    Article Title: Cardiac myosin binding protein C regulates postnatal myocyte cytokinesis
    Article Snippet: .. Primary antibodies included the following: BrdU (rat anti-BrdU, Abcam ab6326, 1:200), Ki67 (rabbit anti-Ki67, Abcam ab15580, 1:200), cTnT (mouse anti-cTnT, Abcam ab8295, 1:200), cardiac troponin-I (rabbit anti-Tnni3, Abcam ab56357, 1:200), WGA (Invitrogen , 1:400), pH3 (rabbit anti-pH3, Millipore 06–570, 1:250), and Aurora B (rabbit anti-Aurora B, Abcam ab2254, 1:250). .. Sections were washed in PBS and fluorophore-conjugated secondary antibody (Molecular Probes) diluted 1:200 in 1% FCS or donkey serum.

    Incubation:

    Article Title: Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells
    Article Snippet: .. The cells then were incubated with the primary antibody anti-cardiac troponin T (cTnT, cat. no. ab19615, Abcam) or HCN4 (cat. no. ab8295; Abcam) overnight at 4°C and incubated with goat anti-mouse or goat anti-rat secondary antibody Alexa Fluor 647 (cat. no. A0473; Beyotime Institute of biotechnology) or Alexa Fluor 647 (cat. no. ab150167; Abcam) for 50 min at room temperature. .. Nuclei stained with 4′,6-diamidino-2-phenylindole was used as a location control.

    Article Title: Nanovesicles derived from iron oxide nanoparticles–incorporated mesenchymal stem cells for cardiac repair
    Article Snippet: .. Then, the slides were incubated with primary antibodies against cardiac troponin T (cTnT), Cx43, CD68, CD206, and NOS2 (these antibodies were purchased from Abcam), diluted with Dako Antibody Diluent Background Reducing Components (Agilent Technologies) at 4°C overnight. .. The slides were washed three times with PBS, incubated for 1 hour with rhodamine-conjugated secondary antibodies at room temperature, washed, mounted with a mounting medium (Vector Laboratories Inc.), and photographed using fluorescent microscope (Olympus).

    Article Title: Accreditation of Biosafe Clinical-Grade Human Embryonic Stem Cells According to Chinese Regulations
    Article Snippet: .. Samples were subsequently incubated with primary antibodies against OCT4 (Santa Cruz Biotechnology, sc-8628, 1:200), SOX2 (Santa Cruz, sc-17320, 1:200), TRA-1-60 (Millipore, MAB4360, 1:200), TRA-1-81 (Millipore, MAB4381A4, 1:200), SSEA4 (Millipore, MC-813-70, 1:200), BEST1 (Abcam, ab14929, 1:150), TH (Santa Cruz, sc-14007, 1:200), PAX6 (Abcam, ab5790, 1:200), OTX2 (Millipore, AB9566, 1:200), MESP1 (Aviva System Biology, OAAB09387, 1:50), CTNT (Abcam, ab8295, 1 μg/mL), TUJ1 (Cell signaling, 2125), LMX1α (Abcam, ab31006, 1:50), SOX17 (R & D Systems, AF1924, 1:200), or HNF4α (Epitomics, 2803-1, 1:200) at 4°C overnight. .. On day 2, the samples were rinsed and then incubated with the appropriate secondary antibodies donkey-anti-mouse-cy5 (Jackson ImmunoResearch, 103856, 1:200), donkey-anti-goat-Cy5 (Millipore, AP1805A6, 1:200), donkey-anti-rabbit-FITC (Jackson ImmunoResearch, 99320, 1:200), or donkey-mouse-cy3 (Jackson ImmunoResearch, 715-165-150, 1:200) for 1 hr at 37°C.

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  • cx43  (Abcam)
    99
    Abcam cx43
    <t>Cx43</t> is present in isolated subsarcolemmal mitoplasts. Mitochondria were prepared by differential centrifugation as described in Methods. An equal amount of protein (30 μg) from distinct isolation fractions — i.e., crude homogenate of isolated myocytes (TP), first pellet with cell debris (P1, discarded), second pellet containing mitochondria (P2), second supernatant (S2, discarded), intact mitochondria (P3), mitoplasts (Mitopl) — was loaded in each lane, and proteins were analyzed by immunoblotting. Immunoreactivity to the following proteins served as markers for cellular compartments: GAPDH (cytosolic marker), Na + /K + -ATPase (plasma membrane marker), SERCA2A (endoplasmic reticulum marker), VDAC (outer mitochondrial membrane marker), UCP2 (inner mitochondrial membrane marker).
    Cx43, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cx43/product/Abcam
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    cx43 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    86
    Abcam polyclonal rabbit anti connexin 43 cx43 primary antibody
    <t>Cx43</t> is present in isolated subsarcolemmal mitoplasts. Mitochondria were prepared by differential centrifugation as described in Methods. An equal amount of protein (30 μg) from distinct isolation fractions — i.e., crude homogenate of isolated myocytes (TP), first pellet with cell debris (P1, discarded), second pellet containing mitochondria (P2), second supernatant (S2, discarded), intact mitochondria (P3), mitoplasts (Mitopl) — was loaded in each lane, and proteins were analyzed by immunoblotting. Immunoreactivity to the following proteins served as markers for cellular compartments: GAPDH (cytosolic marker), Na + /K + -ATPase (plasma membrane marker), SERCA2A (endoplasmic reticulum marker), VDAC (outer mitochondrial membrane marker), UCP2 (inner mitochondrial membrane marker).
    Polyclonal Rabbit Anti Connexin 43 Cx43 Primary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti connexin 43 cx43 primary antibody/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti connexin 43 cx43 primary antibody - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

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    Cx43 is present in isolated subsarcolemmal mitoplasts. Mitochondria were prepared by differential centrifugation as described in Methods. An equal amount of protein (30 μg) from distinct isolation fractions — i.e., crude homogenate of isolated myocytes (TP), first pellet with cell debris (P1, discarded), second pellet containing mitochondria (P2), second supernatant (S2, discarded), intact mitochondria (P3), mitoplasts (Mitopl) — was loaded in each lane, and proteins were analyzed by immunoblotting. Immunoreactivity to the following proteins served as markers for cellular compartments: GAPDH (cytosolic marker), Na + /K + -ATPase (plasma membrane marker), SERCA2A (endoplasmic reticulum marker), VDAC (outer mitochondrial membrane marker), UCP2 (inner mitochondrial membrane marker).

    Journal: The Journal of Clinical Investigation

    Article Title: Connexin 43 acts as a cytoprotective mediator of signal transduction by stimulating mitochondrial KATP channels in mouse cardiomyocytes

    doi: 10.1172/JCI40927

    Figure Lengend Snippet: Cx43 is present in isolated subsarcolemmal mitoplasts. Mitochondria were prepared by differential centrifugation as described in Methods. An equal amount of protein (30 μg) from distinct isolation fractions — i.e., crude homogenate of isolated myocytes (TP), first pellet with cell debris (P1, discarded), second pellet containing mitochondria (P2), second supernatant (S2, discarded), intact mitochondria (P3), mitoplasts (Mitopl) — was loaded in each lane, and proteins were analyzed by immunoblotting. Immunoreactivity to the following proteins served as markers for cellular compartments: GAPDH (cytosolic marker), Na + /K + -ATPase (plasma membrane marker), SERCA2A (endoplasmic reticulum marker), VDAC (outer mitochondrial membrane marker), UCP2 (inner mitochondrial membrane marker).

    Article Snippet: After standard Laemmli SDS-PAGE (12%) and Western blotting (Bio-Rad Tankblot system, nitrocellulose membrane), proteins were detected using the following primary antibodies: Cx43 (1:5,000, rabbit polyclonal, ab11370, Abcam), GAPDH (1:200, rabbit polyclonal IgG, sc-25778, Santa Cruz Biotechnology Inc.), Na+ /K+ -ATPase (1:1,000, rabbit polyclonal IgG, no. 3010, Cell Signaling Technology Inc.), sarcoplasmic reticulum Ca2+ pump gene 2 (SERCA2A; 1:100, goat polyclonal IgG, sc-8094, Santa Cruz Biotechnology Inc.), voltage-dependent anion channel (VDAC; 1:1,000, rabbit polyclonal IgG, no. 4866, Cell Signaling Technology Inc.), uncoupling protein 2 (UCP2; 1:100, goat polyclonal IgG, sc-6525, Santa Cruz Biotechnology Inc.), manganese superoxide dismutase (MN-SOD; 1:1,000, rabbit polyclonal IgG, Upstate), Cx43 phosphorylated at serine 368 (Cx43Ser368; 1:500, rabbit polyclonal IgG, Cell Signaling Technology Inc.), and horseradish peroxidase–coupled anti-rabbit secondary antibody (1:2,000, Sigma-Aldrich).

    Techniques: Isolation, Centrifugation, Marker

    IP increases mitochondrial Cx43 content in Cx43 +/– mice and rescues diazoxide sensitivity of mitoK ATP channels ( A – E ). ( A ) Western blots of Cx43 and MN-SOD in mitochondria from the AW subjected to IP and PW (no IP) of the same Cx43 +/– mouse (IP-induced Cx43 increase normalized to MN-SOD, 4.0 ± 0.9, n = 3). Mitochondrial proteins from wild-type left ventricle (WT LV): positive control; Na + /K + -ATPase: exclusion of contamination with sarcolemmal proteins. ( B ) The diazoxide effect on mitoK ATP activity (at –60 mV) in Cx43 +/– mice was enhanced by IP (right) versus control (left). ( C and E ) Mean values of open probability (Po,total) ( C ) and mean open time ( E ) in PW (black) and AW (IP; white) of Cx43 +/– mice in the absence and presence of diazoxide, as indicated; n values are shown in parentheses. ( D ) Single-channel amplitude as a function of test potentials. Slope conductances were similar in PW and AW (subjected to IP) of Cx43 +/– mice and were unaffected by diazoxide.* P

    Journal: The Journal of Clinical Investigation

    Article Title: Connexin 43 acts as a cytoprotective mediator of signal transduction by stimulating mitochondrial KATP channels in mouse cardiomyocytes

    doi: 10.1172/JCI40927

    Figure Lengend Snippet: IP increases mitochondrial Cx43 content in Cx43 +/– mice and rescues diazoxide sensitivity of mitoK ATP channels ( A – E ). ( A ) Western blots of Cx43 and MN-SOD in mitochondria from the AW subjected to IP and PW (no IP) of the same Cx43 +/– mouse (IP-induced Cx43 increase normalized to MN-SOD, 4.0 ± 0.9, n = 3). Mitochondrial proteins from wild-type left ventricle (WT LV): positive control; Na + /K + -ATPase: exclusion of contamination with sarcolemmal proteins. ( B ) The diazoxide effect on mitoK ATP activity (at –60 mV) in Cx43 +/– mice was enhanced by IP (right) versus control (left). ( C and E ) Mean values of open probability (Po,total) ( C ) and mean open time ( E ) in PW (black) and AW (IP; white) of Cx43 +/– mice in the absence and presence of diazoxide, as indicated; n values are shown in parentheses. ( D ) Single-channel amplitude as a function of test potentials. Slope conductances were similar in PW and AW (subjected to IP) of Cx43 +/– mice and were unaffected by diazoxide.* P

    Article Snippet: After standard Laemmli SDS-PAGE (12%) and Western blotting (Bio-Rad Tankblot system, nitrocellulose membrane), proteins were detected using the following primary antibodies: Cx43 (1:5,000, rabbit polyclonal, ab11370, Abcam), GAPDH (1:200, rabbit polyclonal IgG, sc-25778, Santa Cruz Biotechnology Inc.), Na+ /K+ -ATPase (1:1,000, rabbit polyclonal IgG, no. 3010, Cell Signaling Technology Inc.), sarcoplasmic reticulum Ca2+ pump gene 2 (SERCA2A; 1:100, goat polyclonal IgG, sc-8094, Santa Cruz Biotechnology Inc.), voltage-dependent anion channel (VDAC; 1:1,000, rabbit polyclonal IgG, no. 4866, Cell Signaling Technology Inc.), uncoupling protein 2 (UCP2; 1:100, goat polyclonal IgG, sc-6525, Santa Cruz Biotechnology Inc.), manganese superoxide dismutase (MN-SOD; 1:1,000, rabbit polyclonal IgG, Upstate), Cx43 phosphorylated at serine 368 (Cx43Ser368; 1:500, rabbit polyclonal IgG, Cell Signaling Technology Inc.), and horseradish peroxidase–coupled anti-rabbit secondary antibody (1:2,000, Sigma-Aldrich).

    Techniques: Mouse Assay, Western Blot, Positive Control, Activity Assay