Journal: MethodsX
Article Title: Comparison of three different ELISAs for the detection of recombinant, native and plasma IL-17A
doi: 10.1016/j.mex.2020.100997
Figure Lengend Snippet: Comparison of E_500-P07G, E_41802 and E_eBio64CAP17 for the detection of recombinant and native human IL-17A. (A) Measurement of rhIL-17A standard range, using either the E_500-P07G or E_41802 or E_eBio64CAP17 ELISA, corresponds to the mean of three independent experiments. SD < 10%. (B) Detection of rhIL-17A (1000 pg/mL), rhIL-17E (2000 pg/mL), rhIL-17F (2000 pg/mL) or native IL-17A from PBLs (mean ± S .D, n = 4) by either E_500-P07G or E_41802 or E_eBio64CAP17 ELISA. Supernatants from resting or activated PBLs from four independent donors were evaluated after three days of culture.
Article Snippet: Indeed, 500-P07G PeproTech polyclonal Ab and 41802 R&D Systems monoclonal Ab (mAb) have been ascribed to recognize nonspecific proteins different from IL-17A .
Techniques: Comparison, Recombinant, Enzyme-linked Immunosorbent Assay