Journal: Toxins

Article Title: Development of an Automated Cell-Based Assay for the Detection of the Functional Activity of Saxitoxin

doi: 10.3390/toxins18050206

Figure Lengend Snippet: Evaluation of the neutralising properties of polyclonal IgG towards saxitoxin through measuring functional activity on Nav1.6-HEK cells. ( A ) Representative electrophysiological traces at different concentrations of polyclonal STX IgG with a fixed concentration of 0.1 µM STX. ( B ) Concentration–response curve shows that increasing concentrations of STX polyclonal IgG does not neutralise the Nav1.6 channel blocking effect of 0.1 µM STX at concentrations up to 149 µg/mL. For the STX control, without polyclonal IgG, data were acquired at concentrations of vehicle identical to the matched, IgG-treated, groups. These data are plotted against the equivalent IgG concentration. Each data point for 0.1 µM STX + IgG shows the mean ± SD from 18 cells measured in 3 independent experiments. For comparison, the mean ± SD from 3 cells exposed to 0.1 µM STX measured in 1 experiment is shown.

Article Snippet: STX polyclonal IgG was purchased from Agrisera (Vannus, Sweden).

Techniques: Functional Assay, Activity Assay, Concentration Assay, Blocking Assay, Control, Comparison